Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| plasmodium falciparum transmission blocking monoclonal antibodies recognize monovalently expressed epitopes. | target proteins of transmission blocking monoclonal antibodies (moabs) are present on the surface of plasmodium falciparum macrogametes with mr of 48,000 and 45,000 and on the surface of developing ookinetes with mr of 25,000. other moabs directed against the same proteins were not able to reduce the number of oocysts in mosquitoes. a combination of a blocking moab with a non-blocking one potentiated the transmission blocking effect. this implies that at least two different epitopes are present ... | 1985 | 2422080 |
| genetic studies on malaria parasites. | 1985 | 2444919 | |
| in vitro sensitivity of plasmodium falciparum isolates from burma to chloroquine quinine and mefloquine. | the in vitro sensitivity of 26 isolates of plasmodium falciparum from rangoon and tharrawaddy areas in burma were studied on chloroquine, mefloquine and quinine. the results indicated that the parasites were highly resistant to chloroquine but sensitive to mefloquine and quinine. the existence of correlation of sensitivity to mefloquine and quinine was detected and discussed. no correlation between the parasite sensitivity to chloroquine and mefloquine and or chloroquine and quinine was detected ... | 1985 | 2937157 |
| membrane structure and function of malaria parasites and the infected erythrocyte. | 1985 | 2867515 | |
| structural alteration of the membrane of erythrocytes infected with plasmodium falciparum. | human erythrocytes infected with five strains of plasmodium falciparum and aotus erythrocytes infected with three strains of p. falciparum were studied by thin-section and freeze-fracture electron microscopy. all strains of p. falciparum we studied induced electron-dense conical knobs, measuring 30-40 nm in height and 90-100 nm in diameter on erythrocyte membranes. freeze-fracture demonstrated that the knobs were distributed over the membrane of both human and aotus erythrocytes. a distinct diff ... | 1985 | 2931514 |
| falciparum malaria treated with a fixed combination of mefloquine, sulfadoxine and pyrimethamine: a field study in adults in burma. | in a field study conducted in burma, 54 semi-immune adults suffering from falciparum malaria (mean parasite count, 15 328/mm(3) before treatment) were given a single dose of a fixed combination of 750 mg mefloquine base, 1500 mg sulfadoxine, and 75 mg pyrimethamine (3 tablets of fansimef). all these patients were cleared of asexual parasites by day 7, giving a cure rate of 100%; the mean clearance time was 2.6 days. reappearance of parasitaemia occurred in 10 patients on or before day 7 and pers ... | 1985 | 2935320 |
| expression of plasmodium falciparum circumsporozoite proteins in escherichia coli for potential use in a human malaria vaccine. | the circumsporozoite (cs) protein of the human malaria parasite plasmodium falciparum may be the most promising target for the development of a malaria vaccine. in this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the cs protein were efficiently expressed in the bacterium escherichia coli. when injected into mice, these recombinant products resulted in the production of high titers of antibodies that reacted with the authentic cs protein o ... | 1985 | 2988125 |
| immunogenicity of synthetic peptides from circumsporozoite protein of plasmodium falciparum. | in a study of recombinant proteins that might be useful in developing a vaccine against malaria, synthetic peptides from the circumsporozoite (cs) protein of plasmodium falciparum were found to be immunogenic for mice and rabbits. antibody to peptides from the repeating region of the cs protein recognized native cs protein and blocked sporozoite invasion of human hepatoma cells in vitro. antibodies to peptides from regions i and ii had no biologic activity, although antibody to region i recogniz ... | 1985 | 2988126 |
| plasmodium falciparum: differentiation of isolates with dna hybridization using antigen gene probes. | chromosomal dna was prepared from seven plasmodium falciparum isolates that had been cultured in vitro and from a cloned p. falciparum line. the dna was cleaved with restriction endonucleases, fractionated by agarose gel electrophoresis, blotted to nitrocellulose, and hybridized with a series of radioactively labeled dna probes. the probes had been derived from cdna clones encoding portions of p. falciparum antigens. simple, reproducible band patterns that differed for many of the isolates were ... | 1985 | 2990991 |
| epstein-barr virus serology and malaria exposure in a small group of liberian children with splenomegaly. | 1985 | 2995691 | |
| primary structure of the precursor to the three major surface antigens of plasmodium falciparum merozoites. | recently, a class of protein antigens of high relative molecular mass (mt) which can induce protective immunity against blood-stage malaria has been identified. in plasmodium falciparum the protein has a mr of approximately 195,000 (p195). it is the precursor of three proteins of mr 83,000 (83k), 42k and 19k which are the major surface antigens of merozoites; thus it may also be useful for immunization against p. falciparum. three studies describing the isolation of single short complementary dn ... | 1985 | 2995820 |
| characterization of permeation pathways in the plasma membrane of human erythrocytes infected with early stages of plasmodium falciparum: association with parasite development. | human intraerythrocytic malarial parasites (plasmodium falciparum) induce permeability changes in the membrane of their host cells. the differential permeability of infected erythrocytes at various stages of parasite growth, in combination with density gradient centrifugation, was used to fractionate parasitized cells according to their developmental stage. by this method it was possible to obtain cell fractions consisting essentially of erythrocytes infected with the youngest parasite stage (i. ... | 1985 | 2999164 |
| invasion of erythrocytes in vitro by plasmodium falciparum can be inhibited by monoclonal antibody directed against an s antigen. | a monoclonal antibody has been produced which binds to the heat stable s antigen present in the fcq-27/png isolate of plasmodium falciparum. this monoclonal antibody also inhibits the invasion in vitro of erythrocytes by malarial merozoites thus demonstrating that the s antigens of plasmodium falciparum may be a target of protective immune responses. | 1985 | 2419821 |
| antibody mediated strain-specific agglutination of plasmodium falciparum--parasitized erythrocytes visualized by ethidium bromide staining. | 1985 | 2419822 | |
| geographic diversity of plasmodium falciparum antibodies in madagascar. | plasma samples from 50 subjects living in three distinct regions in madagascar (ankazobe, manakara, and foulpointe) were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis of immunoprecipitated proteins from a plasmodium falciparum strain (fcm22/madagascar) which had been biosynthetically labelled with 35s-methionine and maintained in long-term in-vitro culture. four major proteins with molecular weights of 96, 100, 110, and 118 kd were precipitated by plasma from ankazobe, wh ... | 1985 | 2857815 |
| activity of fusidic acid against plasmodium falciparum in vitro. | 1985 | 2857925 | |
| chloroquine and fansidar resistant malaria acquired in angola. | 1985 | 2858039 | |
| pathophysiological and prognostic significance of cerebrospinal-fluid lactate in cerebral malaria. | cerebrospinal-fluid (csf) lactate concentrations were elevated in all but 1 of 45 patients with cerebral malaria. they were significantly higher in patients who died (9.0 +/- 5.3 mmol/l, mean +/- sd) than in survivors (3.4 +/- 1.1 mmol/l, p = 0.0002) and had returned to normal values in each of 9 patients studied after recovery of consciousness. there was a significant negative correlation between csf lactate and csf glucose. all 11 patients with csf lactate concentrations above 6 mmol/l died. c ... | 1985 | 2858665 |
| trials of mefloquine in vivax and of mefloquine plus 'fansidar' in falciparum malaria. | two randomised double-blind trials were conducted to examine the activity and tolerability of mefloquine alone and in combination with sulfadoxine/pyrimethamine (msp). in one trial mefloquine was compared with chloroquine in 40 patients with plasmodium vivax malaria and in the other one mefloquine was compared with msp in 40 patients with p falciparum malaria. the former trial showed that both a single oral dose of 250 mg mefloquine and a single oral dose of 450 mg chloroquine (base) were highly ... | 1985 | 2858743 |
| effects of transmission-blocking monoclonal antibodies on different isolates of plasmodium falciparum. | the strain diversity in plasmodium falciparum has been studied with respect to gamete surface antigens which are the targets of transmission-blocking antibodies. of 12 isolates tested, 11 were positive by immunofluorescence with the three monoclonal antibodies studied. the exception was a liberian isolate, two clones of which were found to react with only one of the three monoclonal antibodies. antibodies iic5-b10 and ia3-b8, which previously have been shown to act synergistically to block infec ... | 1985 | 2860065 |
| diversity of plasmodium falciparum antibodies in pregnancy. | 1985 | 2860308 | |
| chloroquine-resistant plasmodium falciparum in cameroon. | 1985 | 2860354 | |
| chloroquine-resistant plasmodium falciparum: now in pakistani punjab. | to investigate the susceptibility of plasmodium falciparum to chloroquine in pakistani punjab, in-vivo and in-vitro drug susceptibility was tested in 3 villages. of 66 patients followed for up to 14 days after chloroquine treatment, 53 (80%) had parasites sensitive to chloroquine, 10 (15%) had parasites with early ri resistance, and 3 (5%) had parasites with rii resistance. of 42 falciparum isolates investigated in vitro, 12 were sensitive, 8 intermediate, and 22 resistant to chloroquine. the de ... | 1985 | 2861372 |
| dangers of high-dose quinine and overhydration in severe malaria. | 1985 | 2861399 | |
| chloroquine-resistant malaria in africa. | 1985 | 2861414 | |
| quinine and severe falciparum malaria in late pregnancy. | quinine dihydrochloride was given intravenously to 12 women with severe falciparum malaria in the third trimester of pregnancy. the initial dose consisted of 10 or 20 mg salt/kg over 4 h and was followed by 10 mg salt/kg every 8 h until patients were fit to swallow, when quinine sulphate tablets were given. uterine activity showed little or no change despite rising quinine concentrations. of 3 patients in labour, 2 proceeded normally while a third had a successful caesarean section for fetal dis ... | 1985 | 2861481 |
| is plasmodium falciparum resistance to chloroquine reversible in absence of drug pressure? | 1985 | 2862437 | |
| ri quinine-fansidar resistant falciparum malaria from malawi. | 1985 | 2862492 | |
| [epidemiologic study of malaria in the rice-growing regions of yagoua and maga (north cameroon)]. | the results of the malaria investigation carried out in april 1981 in the irrigated regions of the logone valley show that plasmodium falciparum is the only species o. the genus plasmodium to be found there. in terms of parasitaemia in blood, the prevalence of malaria is highest in the 5 to 9 year-old age group, whereas fluorescent antibodies are to be found progressively greater quantities as subjects grow older. from the age of 40, 95% of the inhabitants of the region present circulating antib ... | 1985 | 2863004 |
| malaria treated with emetine or metronidazole. | 1985 | 2863513 | |
| elisa htlv retrovirus antibody reactivity associated with malaria and immune complexes in healthy africans. | a serological survey of 250 outpatients in rural zaire showed that the prevalence of antibody against htlv-i, htlv-ii, and htlv-iii, as detected by enzyme-linked immunosorbent assay, correlated strongly with level of antibodies against plasmodium falciparum. the age curve for the prevalence of antibody against these retroviruses and high titres of antibodies against p falciparum were similar. tests with control sera obtained from htlv-iii seropositive homosexual men and american subjects repeate ... | 1985 | 2863543 |
| combination of mefloquine with sulfadoxine-pyrimethamine compared with two sulfadoxine-pyrimethamine combinations in malaria chemoprophylaxis. | 1985 | 2863679 | |
| intravenous amodiaquine and oral amodiaquine/erythromycin in the treatment of chloroquine-resistant falciparum malaria. | in eastern thailand, 14 adults with moderately severe falciparum malaria were treated with intravenous amodiaquine dihydrochloride, loading dose 10 mg base/kg infused over 4 h followed by three further intravenous infusions of 5 mg base/kg at 24, 48, and 72 h. all patients were clinically cured--mean fever clearance time 37.8 h (range 24-60), mean parasite clearance time 64.9 h (18-164). there were no serious toxic effects. 33 patients aged over 5 years with uncomplicated falciparum malaria were ... | 1985 | 2864534 |
| plasmodium falciparum: a major health hazard to africans and asians returning home. | 1985 | 2864585 | |
| chloroquine-resistant malaria in mozambique. | 1985 | 2864613 | |
| sequential expression of antigens on sexual stages of plasmodium falciparum accessible to transmission-blocking antibodies in the mosquito. | plasmodium falciparum gametocytes contain specific antigens, some of which (mr 230,000, 48,000, 45,000) are expressed on the surface of the newly emerged macrogamete. a different antigen (mr 25,000) surrounds the surface of the ookinete and, although present to some extent in the developing gametocyte, is synthesized in high quantities by the macrogamete/zygote and expressed progressively on the transforming zygote surface. these antigens are targets of transmission blocking antibodies that are ... | 1985 | 2865324 |
| htlv positivity in africans. | 1985 | 2865454 | |
| rii and riii type resistance of plasmodium falciparum to combination of mefloquine and sulfadoxine/pyrimethamine in indonesia. | 2 of 36 plasmodium falciparum infections were resistant (rii and riii) in vivo to the combination of mefloquine (m) and sulfadoxine-pyrimethamine (sp) in jayapura, irian jaya, indonesia. expected absorption of mefloquine and pyrimethamine was confirmed in the one resistant patient from whom sera were available, and the isolate from this patient was sensitive to mefloquine in vitro. only 2 of 41 infections studied at the same time were resistant in vivo to sp. there was no clinical advantage of m ... | 1985 | 2865518 |
| chloroquine-resistant falciparum malaria in the congo. | 1985 | 2865551 | |
| chloroquine and amodiaquine resistant falciparum malaria in rwanda. | 1985 | 2865553 | |
| nutritional requirements of plasmodium falciparum in culture. iii. further observations on essential nutrients and antimetabolites. | in a semi-defined minimal medium for cultivation of plasmodium falciparum, ribose, mannose, fructose, galactose, and maltose could not replace glucose. hypoxanthine was the preferred purine source for the parasite over adenine, guanine, inosine, adenosine and guanosine although all supported growth equally. inhibitors of nucleoside uptake had low potency in killing the parasites but depressed incorporation of [3h]adenosine more than [3h]hypoxanthine. glutamate could not be replaced by 5-oxoproli ... | 1985 | 2866244 |
| recombinant dna techniques as potential diagnostic means. | 1985 | 2871791 | |
| revised recommendations for preventing malaria in travelers to areas with chloroquine-resistant plasmodium falciparum. | 1985 | 3156271 | |
| leads from the mmwr. revised recommendations for preventing malaria in travelers to areas with chloroquine-resistant plasmodium falciparum. | 1985 | 3157011 | |
| nutritional requirements of plasmodium falciparum in culture. ii. effects of antimetabolites in a semi-defined medium. | a semi-defined minimal medium, in which pantothenic acid is the only vitamin, was used to culture plasmodium falciparum for the analysis of antimetabolite drugs. analogs of riboflavin, nicotinamide, pyridoxine, and thiamin inhibited the growth of this parasite; for each drug, effects were much more pronounced after 96 h of exposure compared to 48 h. the most potent drug examined was 8-methylamino-8-desmethyl riboflavin (ic50 value approximately 1.0 x 10(-10) m at 96 h). avidin, a protein which c ... | 1985 | 3157797 |
| [disorders of the erythrocyte oxidation state in plasmodium falciparum malaria treated with chloroquine]. | 1985 | 3158938 | |
| [severe drop in plasma fibronectin in severe forms of plasmodium falciparum malaria]. | 1985 | 3158994 | |
| [glomerulopathies associated with parasitoses]. | glomerular diseases linked to parasitic infections are frequent in tropical areas: the nephrotic syndrome of african children is often related to malarial infections and hepato-splenic schistosomiasis may be associated with glomerulonephritis. histological studies indicate that the same parasite can induce a spectrum of glomerular lesions. immune complexes play a major role in the pathogenesis of these nephropathies but their precise composition is largely unknown. experimental studies suggest t ... | 1985 | 3160960 |
| polymorphism of a high molecular weight schizont antigen of the human malaria parasite plasmodium falciparum. | intraspecies antigenic diversity in the blood stages of the human malaria parasite plasmodium falciparum was investigated using a collection of murine monoclonal antibodies and clones of the parasite. the results were as follows: (a) the schizont and merozoite stages of the parasite express on their surface clonally restricted antigens detectable by strain-specific antibodies in indirect immunofluorescence tests. (b) these restricted antigens are phenotypically stable characteristics of clones g ... | 1985 | 2578540 |
| x-ray sensitivity and dna synthesis in synchronous culture of plasmodium falciparum. | the relationship between parasite development and sensitivity to irradiation with x-rays was investigated during a single synchronous cycle of plasmodium falciparum in culture. the sensitivity of the parasites to irradiation was closely correlated with the phases of dna synthesis. their sensitivity was greatest at the ring stage in development, but decreased at the trophozoite stage when dna synthesis begins. lowest sensitivity was found when dna synthesis was most rapid as the parasites were tr ... | 1985 | 2581389 |
| ornithine decarboxylase inhibition and the malaria-infected red cell: a model for polyamine metabolism and growth. | the addition of dl-alpha-difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, to human plasmodium falciparum-infected red cells in continuous culture decreased both parasite growth and intracellular polyamine concentrations. growth of p. falciparum in infected red cells was assessed by parasite counts and by assays for macromolecular syntheses of protein, dna and rna. polyamine concentrations were measured by an automated high-performance liquid chromatography method. c ... | 1985 | 2582110 |
| the gene for an exported antigen of the malaria parasite plasmodium falciparum cloned and expressed in escherichia coli. | an exported protein of the erythrocytic stages of the malaria parasite, plasmodium falciparum, has epitope(s) in common with the surface of the sporozoite stage (1). two cdna clones encoding this protein, ag5.1, have now been isolated and expressed in escherichia coli. the coding sequence contains a region with strong homology to that of the circumsporozoite protein of p. falciparum. other features of the sequence can be explained in terms of the observed behaviour of the protein in the parasite ... | 1985 | 2582354 |
| synthesis of small nuclear ribonucleoprotein particles by the malarial parasite plasmodium falciparum. | sera from patients with autoimmune diseases have been used to identify small nuclear ribonucleoprotein particles (snrnps) present in higher eukaryotic cells and also in dinoflagellates. previously these sera have not detected crossreactive snrnp protein antigens of other lower eukaryotes such as yeast, tetrahymena, or dictyostelium. we report that anti-sm, anti-u1-rnp, and anti-la/ss-b human antisera react with specific snrnp protein antigens synthesized by the protozoan plasmodium falciparum, t ... | 1985 | 2582421 |
| human t lymphocyte clones specific for malaria (plasmodium falciparum) antigens. | peripheral blood mononuclear cells (pbm) from a patient who had lived in a malarial-endemic area were cultured in the presence of malarial antigens (a lysate of plasmodium-infected erythrocytes). responding cells were grown in il-2-containing medium and then cloned, and subsequently subcloned, in the presence of phytohemagglutinin and allogeneic irradiated pbm. ten clones were specific for malarial antigens. they proliferated in response to p. falciparum extract, but not to a lysate of uninfecte ... | 1985 | 3004971 |
| polymorphism of the precursor for the major surface antigens of plasmodium falciparum merozoites: studies at the genetic level. | the gene for the precursor of plasmodium falciparum merozoite surface antigens has been cloned. the entire sequence of the gene from a thai isolate of the parasite is reported. it provides evidence for a signal peptide, a region containing short repeating peptides and an anchor sequence. in addition, the 5' end of a papua new guinea isolate has been sequenced. comparison of these and other sequences defines, at the genetic level, a polymorphic region in the protein, and suggests that other parts ... | 1985 | 3004972 |
| organization of plasmodium falciparum genome: 1. evidence for a highly repeated dna sequence. | plasmodium falciparum dna, isolated from the merozoite stage, was cleaved with hindiii and cloned in pbr322 and lambda l47.1 vectors. plasmid clones containing 13.4, 7.0, 4.3, 4.1 and 1.5 kb inserts were characterized in some detail. the inserts contain several repeating units of smaller size. nucleic acid hybridization studies showed that the repeat element is present in the plasmodium dna at a very high copy number and appears to be distributed widely throughout the genome. | 1985 | 4000949 |
| admission of hb s heterozygotes to a general hospital is relatively reduced in malarial areas. | a comparison between the frequency of hb s heterozygotes in blood donors, outpatients, and inpatients of a general hospital carried out at the maputo central hospital, mozambique, where plasmodium falciparum malaria is endemic, showed a statistically significant lower percentage of hb s heterozygotes in the inpatient group. evidence is thus provided that the protection given by hb s to heterozygotes concerns not only malarial infection itself, but probably a wide spectrum of diseases to which pe ... | 1985 | 4045956 |
| a comparison of maternal and foetal folacin and cobalamin activities at parturition in relation to plasmodium falciparum infection. | folacin deficiency in malaria endemic areas is not reported other than in severely malnourished children. this is surprising in view of the high incidence in these areas of malaria in pregnancy, with its complications of prematurity and low birth weight, all of which can predispose to folacin deficiency. this paper reports results of folacin and cobalamin activities of blood samples collected from women at parturition and their newborns in a holoendemic area in west kenya. evidence is given that ... | 1985 | 4095747 |
| sickle cell trait and plasmodium falciparum parasitaemia in pregnancy in western province, kenya. | 1985 | 4095754 | |
| [indications characteristic for different antiparasitic treatments in endemic zones]. | in endemic regions, certain anti-parasitic therapies are automatically prescribed when confronted with apparently benign childhood disorders. the diagnostic differentiation between a simple febrile seizure provoked by plasmodium falciparum is often impossible, requiring the initial use of intravenous quinine. helminth or giardia infestations often aggravate the chronic diarrhea of malnutrition, or are revealed with corticosteroid therapy, necessitating the initiation of an appropriate treatment. ... | 1985 | 3833111 |
| genetic and population structure of four sardinian villages. | data on microgeographic population structure on four neighbouring villages of sardinia island (italy) are presented and discussed. two villages are located in the lowlands where malaria from plasmodium falciparum was endemic until the eradication of paludism. the other two villages are located in the highlands and they were malaria-free because of the altitude. census data, inbreeding, migration matrices and surname distributions have been collected. the genetic differentiation of the four villa ... | 1985 | 3865619 |
| role of the carbohydrate domains of glycophorins as erythrocyte receptors for invasion by plasmodium falciparum merozoites. | solubilized preparations of purified glycophorins and specific domains of these molecules were assessed for their effects as inhibitors of plasmodium falciparum invasion of human erythrocytes in vitro. the ability of newly invaded merozoites to continue developing and incorporating [3h]hypoxanthine during a 24-h period after their invasion was used as an assay for merozoite invasion. glycophorins a, b, and c were found to be equally effective as inhibitors. previous studies had shown n-acetylglu ... | 1985 | 3880722 |
| plasmodium falciparum malaria: band 3 as a possible receptor during invasion of human erythrocytes. | human erythrocyte band 3, a major membrane-spanning protein, was purified and incorporated into liposomes. these liposomes, at nanomolar concentrations of protein, inhibited invasion of human erythrocytes in vitro by the malaria parasite plasmodium falciparum. liposomes containing human band 3 were ten times more effective in inhibiting invasion than those with pig band 3 and six times more effective than liposomes containing human erythrocyte glycophorin. liposomes alone or liposomes containing ... | 1985 | 3880920 |
| complete development of hepatic stages of plasmodium falciparum in vitro. | an in vitro model was developed to study the hepatic phase of plasmodium falciparum, the only malaria parasite lethal to man. primary cultures of human hepatocytes were inoculated with sporozoites of brazilian and african strains of p. falciparum. on days 1 through 7 after inoculation examination of fluorescence-labeled and giemsa-stained preparations demonstrated the presence of many intracellular parasites. in three separate sets of experiments all cultures were found to be infected with as ma ... | 1985 | 3880923 |
| plasmodium falciparum in vitro: diminished growth in hemoglobin h disease erythrocytes. | studies of the ability of plasmodium falciparum to grow in vitro in the red blood cells of subjects with certain beta-thalassemia syndromes are often difficult to interpret because of the known inhibitory effect of an elevated cellular content of human fetal hemoglobin (hbf). p falciparum therefore was cultured in vitro in the erythrocytes of subjects with hemoglobin h (hbh) disease and various other alpha-thalassemia genotypes that are unaccompanied by increased levels of hbf. growth of the mal ... | 1985 | 3881144 |
| plasmodium falciparum malaria mimicking autoimmune hemolytic anemia during pregnancy. | a 30-year-old woman contracted plasmodium falciparum malaria in the first trimester of her pregnancy while taking chloroquine for malaria prophylaxis. her illness was characterized by hemolytic anemia with igg1 coating of the surface of the erythrocytes and igg3 in her serum. the hemolysis subsided following treatment of the malaria infection early in the third trimester. she delivered at term an infant who had hypoplasia of the right tibia and fibula and absence of the fifth ray of the right fo ... | 1985 | 3881157 |
| characterization of one polypeptide antigen potentially related to protective immunity against the blood infection by plasmodium falciparum in the squirrel monkey. | we have previously demonstrated that squirrel monkeys vaccinated with a particular protein fraction of plasmodium falciparum develop a protective immunity that is expressed at the humoral level by the presence of antibodies directed essentially against two parasite proteins. we have now isolated a mab that recognizes one of these polypeptides of an apparent m.w. of 90,000 and an isoelectric point of 6.2. this parasite protein is synthesized in a short period of the asexual blood cycle correspond ... | 1985 | 3881528 |
| characterization with monoclonal antibodies of a surface antigen of plasmodium falciparum merozoites. | the merozoite, the extracellular form of the erythrocyte stage of the malarial parasite, invades the erythrocyte and develops intracellularly. cloned hybridoma cell lines secreting monoclonal antibodies directed against the merozoite surface were selected by indirect immunofluorescent assay by using intact isolated merozoites. monoclonal antibodies to a 200,000 m.w. merozoite surface antigen were selected and were used to characterize this protein and its role in erythrocyte invasion. immunoelec ... | 1985 | 3881529 |
| a role for carbohydrate moieties in the immune response to malaria. | treatment of antigen prepared from asexual blood stages of the human malarial parasite plasmodium falciparum with a mixture of glycosidases resulted in a reduction in the ability of the antigen to bind antibodies from immune human and monkey sera in an elisa assay. some of the epitopes in the parasite material were heat stable, protease resistant, and sensitive to glycosidases. proteins of mr 110,000 and 65,000 from parasitized rbc were shown to have reduced antigenicity in western blots after g ... | 1985 | 3881530 |
| leads from the mmwr. adverse reactions to fansidar and updated recommendations for its use in the prevention of malaria. | 1985 | 3881605 | |
| [diagnostic malaria studies: parasitological or serological?]. | 1985 | 3881684 | |
| interspersed blocks of repetitive and charged amino acids in a dominant immunogen of plasmodium falciparum. | we describe an antigen of plasmodium falciparum that is a dominant immunogen in man. the corresponding cdna clone, ag231, expressing this antigen in escherichia coli reacted in an in situ colony assay with sera from up to approximately equal to 93% of 65 people living in an area in which p. falciparum is endemic. human antibodies affinity purified on immobilized ag231 lysates identified the corresponding parasite antigen as a polypeptide of mr approximately equal to 300,000. it was present in sc ... | 1985 | 3881769 |
| [advantages and disadvantages of different concepts of malaria prevention]. | 1985 | 3881839 | |
| cloning and characterization of plasmodium falciparum fcr-3/fmg strain. | the fcr-3/fmg african strain of plasmodium falciparum was cloned by the limiting dilution technique in in vitro culture to obtain parasite populations derived from a single cell. the basic in vitro culture technique used was that of trager and jensen, performed in microtiter culture plates. the cloning sequence was repeated serially three times, and three parasite clones with a higher than 99% probability of single cell derivation were isolated. two of these clones were determined by electron mi ... | 1985 | 3882009 |
| ultrastructure of the lung in falciparum malaria. | we describe a case of fatal falciparum malaria, with severe pulmonary insufficiency in the absence of fluid overload or cardiac failure. at autopsy the most striking change was a marked pulmonary interstitial edema. the endothelial cell was the most altered structure, showing marked cytoplasmic swelling which narrowed the capillary lumen. monocytes were also found occupying the capillary lumen. the edematous interstitium also showed macrophages with endocytes and malarial pigment. there was no d ... | 1985 | 3882010 |
| parasitologic and immunologic studies of experimental plasmodium falciparum infection in nonsplenectomized chimpanzees (pan troglodytes). | parasitologic, hematologic, and immunologic parameters were monitored in intact (nonsplenectomized), adult chimpanzees infected with a "chimp-adapted" strain of plasmodium falciparum. following primary and secondary injections of 10(9) p. falciparum-infected erythrocytes, each chimpanzee developed a low grade parasitemia (up to 1,000/mm3) and maintained the infection without evidence of eliminating the parasites. hematologic and serum biochemical values, as well as the majority of immunologic pa ... | 1985 | 3882011 |
| structural alteration of the membrane of erythrocytes infected with babesia bovis. | babesia bovis, causative agent of cattle babesiosis, induces characteristic alterations on the membrane of infected erythrocytes. elliptical protrusions measuring about 320 nm in long axis and about 160 nm in short axis appear on the membrane of infected erythrocytes, both in vitro and in vivo. freeze-fracture demonstrated alignment of intramembrane particles (imp) along the long axis of both the p and e faces of the protrusions. the number of imp on the endoplasmic face increases, but the numbe ... | 1985 | 3882012 |
| pathogenicity, stability, and immunogenicity of a knobless clone of plasmodium falciparum in colombian owl monkeys. | the pathogenicity, immunogenicity, and morphological stability of a knobless clone of strain fcr-3 of the human malaria parasite plasmodium falciparum was investigated in aotus monkeys. an early knob-bearing (k+), wild-type isolate of strain fcr-3 and the d3 knobless (k-) clone were adapted to aotus monkey erythrocytes in continuous culture, establishing the parasites in aotus cells without exposure to in vivo cellular or humoral immune responses. all monkeys, intact or splenectomized, which wer ... | 1985 | 3882566 |
| pharmacokinetics of quinine in children. | serum quinine concentrations were measured in seven children after intravenous infusion of quinine dihydrochloride, in eight children after intramuscular injection of quinine dihydrochloride, and in six children after nasogastric administration of a solution of quinine dihydrochloride. the mean (+/- sd) half-life of quinine was 11.1 +/- 4.8 hours, and the volume of distribution was 1.39 +/- 0.37 l/kg. to attain a serum level of 10 microgram/ml quinine, we suggest that children with severe malari ... | 1985 | 3882924 |
| inadequate prophylaxis of malaria with dapsone-pyrimethamine. | the dapsone-pyrimethamine combination (100 mg of dapsone, 12.5 mg of pyrimethamine [folaprim; maloprim, one tablet a week) is considered to provide adequate prophylaxis for plasmodium falciparum malaria, but to be inadequate for the prevention of p. vivax malaria. field trials and case reports, however, have shown the comparable efficacy of this combination in the suppression of parasitaemias caused by both parasites. in lae, papua new guinea, 12 patients with clinical signs of malaria had serum ... | 1985 | 3883112 |
| adaptation of plasmodium falciparum to glucose 6-phosphate dehydrogenase-deficient host red cells by production of parasite-encoded enzyme. | there is impressive evidence from geographical data, studies in the field and in vitro culture work that genetically determined deficiency of glucose 6-phosphate dehydrogenase (g6pd) confers relative protection against the human malaria parasite, plasmodium falciparum. g6pd is encoded by an x-chromosome-linked gene, and protection phenomenon is manifested in heterozygous females who are genetic mosaics but, surprisingly, not in hemizygous males with complete deficiency. we have shown previously ... | 1985 | 3883193 |
| isolation of the gene for a glycophorin-binding protein implicated in erythrocyte invasion by a malaria parasite. | plasmodium falciparum, the most lethal of the malarial parasites that infect humans, undergoes three cycles of development in its vertebrate host and elicits stage-specific immune responses. this stage specificity of the immune response has made it difficult to isolate antigens that would be useful in developing a vaccine against malaria. a complementary dna clone for a glycophorin-binding protein of plasmodium falciparum merozoites has been isolated and characterized. the protein interacts with ... | 1985 | 3883491 |
| evidence for a malarial parasite interaction site on the major transmembrane protein of the human erythrocyte. | soluble oligosaccharides derived from the surface of human erythrocytes were tested for their ability to competitively inhibit invasion of erythrocytes by plasmodium falciparum, a malarial parasite. invasion was most effectively inhibited by erythroglycan, a carbohydrate component of the band 3 transmembrane protein. the lactosamine chains of erythroglycan contributed much of the inhibitory activity. this indication of a primary parasite interaction site on band 3 supports a role for this protei ... | 1985 | 3883494 |
| specific proliferative response of human lymphocytes to purified soluble antigens from plasmodium falciparum in vitro cultures and to antigens from malaria patients' sera. | antigens of plasmodium falciparum, in supernatants of in vitro cultures of the parasite were affinity purified on columns prepared with the igg fraction of the serum of an immune individual. the purified antigens induced proliferation of lymphocytes from persons who had recently had malaria. the responses were strongest with lymphocytes from individuals infected with falciparum and ovale malaria; vivax malaria infections induced a lower level of response and lymphocytes of unsensitized individua ... | 1985 | 3884198 |
| recognition of a mr 56k glycoprotein on the surface of plasmodium falciparum merozoites by mouse monoclonal antibodies. | hybridomas were prepared from mice repeatedly injected with disrupted plasmodium falciparum (fvo isolate) schizonts and merozoites. antibodies secreted by two of these hybridomas were shown by immunoelectron microscopy to bind to the surface of merozoites from the fvo isolate. these monoclonal antibodies (mcab) reacted with the fvo and geneva isolates by an indirect fluorescence antibody test (ifat) and immunoprecipitated a protein of relative molecular weight (mr) 56k from both isolates. the 56 ... | 1985 | 3884711 |
| malaria prophylaxis for travellers. | 1985 | 3884983 | |
| antagonism of sulfadoxine and pyrimethamine antimalarial activity in vitro by p-aminobenzoic acid, p-aminobenzoylglutamic acid and folic acid. | the activity of pyrimethamine and sulfadoxine against two strains of plasmodium falciparum has been studied in vitro by a radioisotopic technique. low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. sulfadoxine activity was antagonised slightly by p-aminobenzoic but not by p-aminobenzoylglutamic acid, and antagonised markedly by folic acid at concentrations above 4 x 10(-8) m. at 10(-7) m folic ac ... | 1985 | 3885030 |
| inhibition of pyrimidine biosynthesis de novo in plasmodium falciparum by 2-(4-t-butylcyclohexyl)-3-hydroxy-1,4-naphthoquinone in vitro. | the effects of the hydroxynaphthoquinone bw58c on some metabolite levels and the flux of h14co3 through the de novo pyrimidine biosynthetic pathway of intact plasmodium falciparum have been studied in vitro using hplc techniques. 800 nm bw58c appeared to have no significant effect on the energy status of isolated p. falciparum, but at 0.1 nm it caused a dramatic decrease in the concentrations of pyrimidine nucleotides, specifically utp, during 256 min of incubation. although about one hour was r ... | 1985 | 3885032 |
| [a case of malaria tropicameningoencephalitis (neuromalaria) with chloroquine and pyrimethamine/sulfadoxine resistance]. | 1985 | 3885058 | |
| malaria in pregnancy. | malaria is a potentially lethal disease. in pregnancy its deleterious effects are aggravated by the depressed immune state. every pregnant patient presenting with pyrexia of unknown origin should be screened for malarial parasites, especially if she has travelled to or come from an area in which malaria is endemic. case histories of 2 pregnant women with malaria are presented and the diagnosis, treatment and chemoprophylaxis thereof are discussed. | 1985 | 3885422 |
| the relationship to knobs of the 92,000 d protein specific for knobby strains of plasmodium falciparum. | a 92,000 d protein was identified associated with the membrane of host erythrocytes infected with the fcb1 plasmodium falciparum strain from colombia. the same protein was identified in the knob-forming gambian (and the malayan camp) strain, but was not present in all the corresponding knobless strains. in the fcb1 strain as well as in the fcr3 strain the protein is synthesized during the ring-stage period. the cleavage products of the 92,000 d protein were investigated by peptide mapping follow ... | 1985 | 3885605 |
| cryopreservation of the blood stages of plasmodium falciparum and plasmodium vivax for in vivo studies. | cryopreservation in alsevers-glycerol of the blood stages of plasmodium falciparum (5 strains) and p. vivax (2 strains) indicated that parasite infectivity for aotus was retained for more than 1,100 days. | 1985 | 3885767 |
| use of non-human plasma for in vitro cultivation and antimalarial drug susceptibility testing of plasmodium falciparum. | viability, growth rate, and chemotherapeutic susceptibility of the cdc/indochina iii, cdc/sierra leone i, and fcr-3 (subline f-86) isolates of plasmodium falciparum grown continuously in rpmi 1640 medium supplemented with goat, horse, porcine, bovine, or ovine plasma were evaluated. results were compared to those obtained from parallel cultures maintained in medium supplemented with non-immune human plasma. only media supplemented with goat or horse plasma supported significant continuous multip ... | 1985 | 3885768 |
| toxic heme in sickle cells: an explanation for death of malaria parasites. | in an effort to elucidate a mechanism of genetic resistance to malaria, we asked whether a toxic form of heme is included in the excess of ferriprotoporphyrin ix (fp) which has been reported to accumulate as hemichromes in sickle cells. when fp is bound to certain erythrocytic elements, such as native hemoglobin, it is inaccessible to bind chloroquine with high affinity and is nontoxic. however, when fp is accessible to bind chloroquine with high affinity, it has been demonstrated to be sufficie ... | 1985 | 3885769 |
| antimalarial drug susceptibility testing of plasmodium falciparum in thailand using a microdilution radioisotope method. | antimalarial activity of chloroquine, quinine, mefloquine and halofantrine against 33 strains of p. falciparum isolated from naturally acquired malaria infections in thailand was determined using a radioisotope microdilution method. a microtitration procedure was used to test isolates of p. falciparum against the 4 drugs simultaneously. the mean id50 for chloroquine and quinine reflected known resistance to those drugs in thailand. the mean id50 for mefloquine and halofantrine showed susceptibil ... | 1985 | 3885770 |
| oxygen- and time-dependent effects of antibiotics and selected mitochondrial inhibitors on plasmodium falciparum in culture. | several antibiotics which inhibit protein synthesis on 70s ribosomes, including clindamycin, pirlimycin, 4'-pentyl-n-demethyl clindamycin, four tetracyclines, chloramphenicol, thiamphenicol, and erythromycin, had antimalarial effects against plasmodium falciparum in culture which were greatly influenced by the duration of drug exposure and by oxygen tension. in 96-h incubations, potency was increased by a factor of up to 10(6) over the first 48-h period and by a factor of up to 10(4) in 15% o2 v ... | 1985 | 3885844 |
| malaria and haemoglobin genotype in young northern nigerian children. | plasmodium falciparum and p. malariae were found less frequently in blood obtained from nigerian children under the age of two years who had the haemoglobin genotype as than in blood films obtained from those who had the genotype aa. however, differences between the two groups were statistically significant only for p. falciparum asexual forms. the prevalence of malaria antibodies, as measured by an elisa, did not differ significantly between the two groups. | 1985 | 3885877 |
| transient total blindness from quinine therapy. | 1985 | 3885879 | |
| prevention and treatment of malaria. | 1985 | 3885928 | |
| conserved sequences flank variable tandem repeats in two s-antigen genes of plasmodium falciparum. | we describe the isolation of two chromosomal dna fragments from plasmodium falciparum. these fragments encode the antigenically distinct s antigens of two different p. falciparum isolates, namely fc27 from papua new guinea and nf7 from ghana. the complete nucleotide sequences of both fragments are presented. the fragments are homologous over most of their lengths, including the entire regions flanking the protein coding sequences. whereas the n- and c-terminal portions of sequences encoding the ... | 1985 | 3886159 |