Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| fungal sterol c22-desaturase is not an antimycotic target as shown by selective inhibitors and testing on clinical isolates. | inhibition of concise enzymes in ergosterol biosynthesis is one of the most prominent strategies for antifungal chemotherapy. nevertheless, the enzymes sterol c5-desaturase and sterol c22-desaturase, which introduce double bonds into the sterol core and side chain, have not been fully investigated yet for their potential as antifungal drug targets. lathosterol side chain amides bearing n-alkyl groups of proper length are known as potent inhibitors of the enzymes sterol c5-desaturase and sterol δ ... | 2015 | 26022150 |
| susceptibility to echinocandins of candida spp. strains isolated in italy assessed by european committee for antimicrobial susceptibility testing and clinical laboratory standards institute broth microdilution methods. | the echinocandins are recommended as first-line therapy for candida species infections, but drug resistance, especially among candida glabrata, is becoming more frequent. we investigated the antifungal susceptibility of anidulafungin, caspofungin, and micafungin against 584 isolates of candida spp. (bloodstream, other sterile sites) collected from patients admitted to an italian university hospital between 2000 and 2013. the susceptibility was evaluated using the broth microdilution method accor ... | 2015 | 25990252 |
| expression vectors for c-terminal fusions with fluorescent proteins and epitope tags in candida glabrata. | candida glabrata is a haploid yeast considered the second most common of the candida species found in nosocomial infections, accounting for approximately 18% of candidemias worldwide. even though molecular biology methods are easily adapted to study this organism, there are not enough vectors that will allow probing the transcriptional and translational activity of any gene of interest in c. glabrata. in this work we have generated a set of expression vectors to systematically tag any gene of in ... | 2015 | 25986172 |
| comments on "candida glabrata candidemia; an emerging threat in critically ill patients". | 2015 | 25983444 | |
| tipping the balance both ways: drug resistance and virulence in candida glabrata. | among existing fungal pathogens, candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. moreover, resistance to the class of echinocandins, which was introduced in the late 1990s, is rising in several institutions. the recent emergence of isolates with acquired resistance to both classes of agents i ... | 2015 | 25979690 |
| anti-vancomycin-resistant enterococcus faecium and e. faecalis activities of (-)-gossypol and derivatives from thespesia garckeana. | the root extract of thespesia garckeana yielded three known oxidatively coupled sesquiterpenoids, namely (-)-gossypol (1) and two of its derivatives (-)-6-methoxygossypol (2) and (+)-6,6'-dimethoxygossypol (3), and the stem bark afforded (e)-docosyl-3-(3,4-dihydroxyphenyl) acrylate (4), stigmasterol (5) and betulinic acid (6). the structures of the isolated compounds were determined on the basis of full spectral data (1d and 2d nmr and hrms) and comparison with literature values. compound 1 show ... | 2015 | 25973489 |
| stress response and expression of fluconazole resistance associated genes in the pathogenic yeast candida glabrata deleted in the cgpdr16 gene. | in yeasts, the pdr16 gene encodes a phosphatidylinositol transfer protein which belongs to the sec14 homologue (sfh) family and localizes to lipid droplets, microsomes and at the cell periphery. the loss of its function alters the lipid droplet metabolism and plasma membrane properties, and renders yeast cells more sensitive to azole antimycotics. in this study, the entire chromosomal cgpdr16 orf was replaced by the scura3 gene both in azole sensitive and azole resistant strains of candida glabr ... | 2015 | 25946325 |
| induction of ros generation by fluconazole in candida glabrata: activation of antioxidant enzymes and oxidative dna damage. | in this study, we assessed the generation of reactive oxygen species (ros) induced by subinhibitory concentration of fluconazole in susceptible and resistant candida glabrata strains at stationary growth phase and measured their oxidative responses parameters: glutathione peroxidase (gpx), superoxide dismutase (sod), glutathione-s-transferase (gst), consumption of hydrogen peroxide, and total glutathione, as well as oxidative damage in lipids, proteins, and dna. data showed that fluconazole incr ... | 2015 | 25935629 |
| exogenous folates stimulate growth and budding of candida glabrata. | folate, vitamin b9, is well recognized as being essential for cell growth. the utilization of folate is common to all cells, but the source of it may be quite different. for example, mammalian cells depend on exogenous uptake of folates, while plants and microbes can synthesize them. there has been little consideration of uptake of folate in microbial cells, and studies on the effects of folates in mammalian cells, where conditions are restricted. this study shows that exogenous folates (folic a ... | 2015 | 28357288 |
| antifungal activity of ellagic acid in vitro and in vivo. | ellagic acid (ea) has been shown to have antioxidant, antibacterial, and anti-inflammatory activities. in uighur traditional medicine, euphorbia humifusa willd is used to treat fungal diseases, and recent studies suggest that it is the ea content which is responsible for its therapeutic effect. however, the effects of ea on antifungal activity have not yet been reported. this study aimed to investigate the inhibitory effect of ea on fungal strains both in vitro and in vivo. the minimal inhibitor ... | 2015 | 25919446 |
| phytase-producing capacity of yeasts isolated from traditional african fermented food products and phypk gene expression of pichia kudriavzevii strains. | phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. ninety-three yeast isolates from traditional african fermented food products, belonging to nine species (pichia kudriavzevii, saccharomyces cerevisiae, clavispora lusitaniae, kluyveromyces marxianus, millerozyma farinosa, candida glabrata, wickerhamomyces anomalus, hanseniaspora guilliermondii and debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were a ... | 2015 | 25910031 |
| candida glabrata fungaemia at an australian cancer centre: epidemiology, risk factors and therapy. | 2015 | 25907425 | |
| the ever-evolving landscape of candidaemia in patients with acute leukaemia: non-susceptibility to caspofungin and multidrug resistance are associated with increased mortality. | the epidemiology and clinical course of candidaemia in patients with acute leukaemia, a population frequently exposed to antifungals, have not been extensively studied. in the present contemporary series of acute leukaemia patients, we describe patient characteristics, candida species and mic distributions and investigate the association between antifungal resistance and all-cause mortality. | 2015 | 25855759 |
| maldi-tof typing highlights geographical and fluconazole resistance clusters in candida glabrata. | utilizing matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectra for candida glabrata typing would be a cost-effective and easy-to-use alternative to classical dna-based typing methods. this study aimed to use maldi-tof for the typing of c. glabrata clinical isolates from various geographical origins and test its capacity to differentiate between fluconazole-sensitive and -resistant strains.both microsatellite length polymorphism (mlp) and maldi-tof mass spectra of 5 ... | 2015 | 25841053 |
| early-onset candida glabrata interface keratitis after deep anterior lamellar keratoplasty. | fungal interface keratitis by candida species can occur several weeks to several months after deep anterior lamellar keratoplasty (dalk). here, we report a case of early-onset fungal interface keratitis by candida glabrata after dalk. | 2015 | 25822017 |
| candida glabrata candidemia: an emerging threat in critically ill patients. | candidemia is an important nosocomial blood stream infection in critically ill patients. although several studies have addressed candidemia, very few have reviewed the impact of candida glabrata candidemia in intensive care unit (icu) patients. | 2015 | 25810610 |
| candida glabrata candidemia. | 2015 | 25810607 | |
| significantly improved thermostability of a reductase cgkr1 from candida glabrata with a key mutation at asp 138 for enhancing bioreduction of aromatic α-keto esters. | the keto ester reductase from candida glabrata, designated as cgkr1, is a highly versatile biocatalyst with a broad substrate spectrum. its substrate preference was altered by rational design of the active pocket for bioreduction of aromatic α-keto esters in our previous work. however, its practical application is still hindered by its poor thermostability and high biocatalyst loading. in this work, random mutagenesis followed by a saturation mutagenesis was performed aiming to improve its therm ... | 2015 | 25795440 |
| of mice, flies--and men? comparing fungal infection models for large-scale screening efforts. | studying infectious diseases requires suitable hosts for experimental in vivo infections. recent years have seen the advent of many alternatives to murine infection models. however, the use of non-mammalian models is still controversial because it is often unclear how well findings from these systems predict virulence potential in humans or other mammals. here, we compare the commonly used models, fruit fly and mouse (representing invertebrate and mammalian hosts), for their similarities and deg ... | 2015 | 25786415 |
| the mitogen-activated protein kinase cghog1 is required for iron homeostasis, adherence and virulence in candida glabrata. | candida glabrata has emerged as a major fungal pathogen over the last two decades, although our understanding of its survival strategies inside the mammalian host remains rudimentary. an important requirement for survival in vivo is the ability to acquire critical nutrients such as iron from host niches of varied iron content. in the present study, we demonstrate for the first time that c. glabrata cells respond to high external iron levels via activation of two stress-responsive mitogen-activat ... | 2015 | 25772226 |
| structural and mechanistic analysis of the slx1-slx4 endonuclease. | the slx1-slx4 endonuclease required for homologous recombination and dna repair in eukaryotic cells cleaves a variety of branched dna structures. the nuclease subunit slx1 is activated by association with a scaffolding protein slx4. at the present time, little is known about the structure of slx1-slx4 or its mechanism of action. here, we report the structural insights into slx1-slx4 by detailing the crystal structure of candida glabrata (cg) slx1 alone and in combination with the c-terminal regi ... | 2015 | 25753413 |
| avoiding the ends: internal epitope tagging of proteins using transposon tn7. | peptide tags fused to proteins are used in a variety of applications, including as affinity tags for purification, epitope tags for immunodetection, or fluorescent protein tags for visualization. however, the peptide tags can disrupt the target protein function. when function is disrupted by fusing a peptide to either the n or c terminus of the protein of interest, identifying alternative ways to create functional tagged fusion proteins can be difficult. here, we describe a method to introduce p ... | 2015 | 25745023 |
| yap7 is a transcriptional repressor of nitric oxide oxidase in yeasts, which arose from neofunctionalization after whole genome duplication. | flavohemoglobins are the main detoxifiers of nitric oxide (no) in bacteria and fungi and are induced in response to nitrosative stress. in fungi, the flavohemoglobin encoding gene yhb1 is positively regulated by transcription factors which are activated upon no exposure. in this study, we show that in the model yeast saccharomyces cerevisiae and in the human pathogen candida glabrata, the transcription factor yap7 constitutively represses yhb1 by binding its promoter. consequently, yap7 deletion ... | 2015 | 25732006 |
| mitochondrial engineering of the tca cycle for fumarate production. | microbial fumarate production from renewable feedstock is a promising and sustainable alternative to petroleum-based chemical synthesis. here, mitochondrial engineering was used to construct the oxidative pathway for fumarate production starting from the tca cycle intermediate α-ketoglutarate in candida glabrata. accordingly, α-ketoglutarate dehydrogenase complex (kgd), succinyl-coa synthetase (suclg), and succinate dehydrogenase (sdh) were selected to be manipulated for strengthening the oxidat ... | 2015 | 25708514 |
| terminal-stage prognostic analysis in candidemia. | candidemia has an extremely high mortality rate but is not always the direct cause of death. therefore, determining the effect of candidemia on death is extremely difficult. | 2015 | 25697387 |
| [evaluation of the rapid trehalose test for the identification of the candida glabrata]. | to explore a rapid and cost-effective method for identification of candida glabrata through the comparison of two different methods, using molecular methods of sequencing as gold standard. | 2015 | 25686354 |
| early initiation of appropriate treatment is associated with increased survival in cancer patients with candida glabrata fungaemia: a potential benefit from infectious disease consultation. | in patients with malignancies, candida glabrata is one of the most frequent non-albicans candida clinical isolates. as antifungal resistance in c. glabrata is common, we investigated the relationship between early appropriate antifungal treatment, infectious disease (id) consultation and mortality in a contemporary cohort of cancer patients with c. glabrata fungaemia. we included patients with at least one c. glabrata-positive blood culture and symptoms or signs of infection seen at the md ander ... | 2015 | 25636931 |
| force nanoscopy of hydrophobic interactions in the fungal pathogen candida glabrata. | candida glabrata is an opportunistic human fungal pathogen which binds to surfaces mainly through the epa family of cell adhesion proteins. while some epa proteins mediate specific lectin-like interactions with human epithelial cells, others promote adhesion and biofilm formation on plastic surfaces via nonspecific interactions that are not yet elucidated. we report the measurement of hydrophobic forces engaged in epa6-mediated cell adhesion by means of atomic force microscopy (afm). using singl ... | 2015 | 25621738 |
| determination of micing: a new assay for assessing minimal inhibitory concentration for invasive growth. | our work was focused on a new assay for characterising clinically important yeast. this assay was developed due to the need for new diagnostic methods for recognising potentially virulent strains of increasingly important non-albicans yeast pathogens, such as saccharomyces cerevisiae and candida glabrata. with the great diversity among strains for virulence and virulence factors, identification to the species level is not sufficient; therefore, testing for specific virulent traits remains the be ... | 2015 | 25620781 |
| a novel candida glabrata cell wall associated serine protease. | we set out to identify the candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. we studied a clinical c. glabrata strain t-1639, which was isolated from a patient from the helsinki university central hospital. with non-reducing 2-d electrophoresis using parallel fluorogenic gels and mass spectrometry we identified a novel appr. 25 kda (192 aa in length) cell wall located protease with an estimated pi o ... | 2015 | 25617734 |
| detection and quantification of fluconazole within candida glabrata biofilms. | candida infections are often associated with biofilms and consequent high resistance to most common drugs (e.g. azoles). these resistance mechanisms are not only associated with the biofilm yeast physiology, but also with the presence of a diffusional barrier imposed by the biofilm matrix; however, the real biochemical role of the biofilm components remains very unclear. so, in order to further clarify this issue, we intend to determine, for the first time, fluconazole in biofilms within both su ... | 2015 | 25614085 |
| candida glabrata and candida tropicalis in an immunocompetent patient: a case report. | to report a case of candida glabrata and tropicalis pneumonia in an immunocompetent patient. | 2015 | 25613052 |
| the epa2 adhesin encoding gene is responsive to oxidative stress in the opportunistic fungal pathogen candida glabrata. | candida glabrata has emerged as an important opportunistic pathogen in both mucosal and bloodstream infections. c. glabrata contains 67 adhesin-like glycosylphosphatidylinositol-cell-wall proteins (gpi-cwps), which are classified into seven groups and the largest is the epa family. epa proteins are very diverse and their expression is differentially regulated. like many of the epa genes, epa2 is localized in a subtelomeric region where it is subject to chromatin-based transcriptional silencing a ... | 2015 | 25586543 |
| in vitro evaluation of the acquisition of resistance, antifungal activity and synergism of brazilian red propolis with antifungal drugs on candida spp. | to evaluate the ability of candida parapsilosis and candida glabrata to develop phenotypic resistance to a benzophenone enriched fraction obtained from brazilian red propolis (bzp-brp) as compared to fluconazole (flc). to investigate possible synergy between bzp-brp and flc and anidulafungin (and). | 2015 | 25565139 |
| emergence of multiple resistance profiles involving azoles, echinocandins and amphotericin b in candida glabrata isolates from a neutropenia patient with prolonged fungaemia. | 2015 | 25550394 | |
| candidemia in intensive care unit patients: a one year study from a tertiary care center in south india. | the occurrence of candidemia is on a rise worldwide. non-albicans candida species have emerged as major causes of candidemia in many countries. added to it is the problem of antifungal resistance in candida isolates. | 2015 | 24029196 |
| inhibitory activity in vitro of probiotic lactobacilli against oral candida under different fermentation conditions. | clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral candida species. sugar utilisation by both lactobacilli and candida was also assessed. agar overlay assay was utilised to study growth inhibition of candida albicans, candida glabrata and candida krusei by lactobacillus rhamnosus gg, ... | 2015 | 25380800 |
| oral candida carriage and prevalence of candida species among maras powder users and non-users. | the aim of this study was to investigate candida carriage and species in maras powder users and non-users. | 2015 | 25213478 |
| changing epidemiology of candidemia in a medical center in middle taiwan. | candidemia remains a major cause of morbidity and mortality in the health care setting, and the epidemiology of candida infection is changing. | 2015 | 24113067 |
| host factors and clinical outcomes of candida colonization in critically ill patients. | this study aimed to describe factors and outcomes associated with candida colonization of critically ill patients. this was a cross-sectional study conducted over 2 weeks in the intensive care unit (icu) of a tertiary care hospital at the texas medical center, houston, tx. all candida samples were prospectively collected with demographic and clinical data collected retrospectively. we examined 48 patients, 32 (67%) were colonized with candida spp; 25 (52.1%) patients were isolated with candida a ... | 2015 | 25173925 |
| susceptibility of candida albicans and candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases. | the aim of this study was to investigate the susceptibility of candida albicans and candida glabrata biofilm development, in their intermediate and maturation stages, to the influence of silver nanoparticles (sn). | 2015 | 25168655 |
| simultaneous detection of four common oral candida species from blood samples by the fluorescence polarization assay. | the genus candida is both the commensal microbe and the opportunistic pathogen, containing approximately 200 species inhabiting in oral cavity of 53 % of the general population. candida species can cause the diseases from local mucosal infections to systemic mycoses, even life-threatening infections in immunocompromised individuals. the timely differentiation of candida species is important for the guidance of clinical medication. four common candida species in chinese population (candida albica ... | 2015 | 25308858 |
| in vitro anti-yeast activity of chloramphenicol: a preliminary report. | chloramphenicol is a bacteriostatic antimicrobial agent but its antifungal activity is not known. the present study aimed to investigate the activity of chloramphenicol against 30 representative yeasts. the antimicrobial assay of chloramphenicol (50mg/ml; 100mg/ml and 200mg/ml) was determined by the disc diffusion method using mueller-hinton agar against 30 representative yeast strains. zone of inhibition was read after 48-72h incubation at 37°c and results were compared with some standard antif ... | 2015 | 25497707 |
| in vivo candida glabrata biofilm development on foreign bodies in a rat subcutaneous model. | biofilm studies have been mostly dedicated to the major human fungal pathogen candida albicans, whereas much less is known about this virulence factor in candida glabrata, certainly under in vivo conditions. this study provides a deeper understanding of the biofilm development of c. glabrata, its architecture and susceptibility profile to fluconazole and echinocandins. | 2015 | 25406296 |
| surveillance of antifungal susceptibilities in clinical isolates of candida species at 36 hospitals in china from 2009 to 2013. | the purpose of this study was to determine the species distribution and to monitor the antifungal susceptibility profiles of clinical candida isolates collected in china from 2009 to 2013. | 2015 | 25541294 |
| species spectrum and antifungal susceptibility profile of vaginal isolates of candida in kuwait. | the study was undertaken to determine the prevalence of vulvovaginal candidiasis (vvc) among patients with vaginitis, frequency of different candida species, and their susceptibility profile. | 2015 | 25534676 |
| lab-scale preparations of candida albicans and dual candida albicans-candida glabrata biofilms on the surface of medical-grade polyvinyl chloride (pvc) perfusion tube using a modified gravity-supported free-flow biofilm incubator (gs-ffbi). | the assembly of a man-made gravity-supported free-flow biofilm incubator (gs-ffbi) was described, which was composed of a gas cushion injector and four incubators. the gs-ffbi had the characteristics of (i) a bottom-up flow direction, and (ii) lab-scale biofilm preparation without the use of a multichannel pump. two opportunistic fungal strains, namely candida albicans and candida glabrata, were employed to incubate c. albicans and dual c. albicans-c. glabrata biofilms on the surface of medical- ... | 2015 | 25528294 |
| lupin peptone as a replacement for animal-derived peptone in rich culture media for yeast. | lupin peptone was shown to be a suitable replacement for traditional bacteriological peptone in the culture of candida glabrata, candida albicans and saccharomyces cerevisiae. this new medium formulation allows yeast researchers to increase safety and to eliminate the use of animal products for the culture of yeast in rich medium. | 2015 | 25514068 |
| effects of bovine lactoferrin to oral candida albicans and candida glabrata isolates recovered from the saliva in elderly people. | the effects of bovine lactoferrin (blf) on the growth of candida species and on inflammatory cytokine production in gingival keratinocytes, ndusd-1 co-cultured with candida strains were investigated. the results showed that blf at 10 and 100 lg/ml significantly inhibits the growth of two c. albicans strains and two c. glabrata strains isolated from the saliva of elderly people requiring nursing care, respectively. the levels of inflammatory cytokines, interleukin (il)-6, and il-8 in ndusd-1 cocu ... | 2015 | 25756168 |
| [fungal infections and ureteral material: how to manage?]. | urinary tract infections due to candida species are mostly encountered in hospital environment. the management of candiduria on ureteral catheter is not consensual. the objective of our work was to make a review of medical literature related to definition, physiopathology, management and prevention of candiduria on ureteral catheter. | 2015 | 25724861 |
| guideline: vulvovaginal candidosis (awmf 015/072), s2k (excluding chronic mucocutaneous candidosis). | the oestrogenised vagina is colonised by candida species in at least 20% of women; in late pregnancy and in immunosuppressed patients, this increases to at least 30%. in most cases, candida albicans is involved. host factors, particularly local defence mechanisms, gene polymorphisms, allergies, serum glucose levels, antibiotics, psycho-social stress and oestrogens influence the risk of candidal vulvovaginitis. non-albicans species, particularly candida glabrata, and in rare cases also saccharomy ... | 2015 | 25711406 |
| declining incidence of candidemia and the shifting epidemiology of candida resistance in two us metropolitan areas, 2008-2013: results from population-based surveillance. | recent reports have demonstrated a decline in bacterial bloodstream infections (bsis) following adherence to central line insertion practices; however, declines have been less evident for bsis due to candida species. | 2015 | 25822249 |
| mechanisms of azole resistance among clinical isolates of candida glabrata in poland. | candida glabrata is currently ranked as the second most frequently isolated aetiological agent of human fungal infections, next only to candida albicans. in comparison with c. albicans, c. glabrata shows lower susceptibility to azoles, the most common agents used in treatment of fungal infections. interestingly, the mechanisms of resistance to azole agents in c. albicans have been much better investigated than those in c. glabrata. the aim of the presented study was to determine the mechanisms o ... | 2015 | 25818698 |
| essential oils, silver nanoparticles and propolis as alternative agents against fluconazole resistant candida albicans, candida glabrata and candida krusei clinical isolates. | development of effective and safe therapeutic treatment of fungal infections remains one of the major challenge for modern medicine. the aim of presented investigation was to analyze the in vitro antifungal activity of selected essential oils, ethanolic extracts of propolis and silver nanoparticles dropped on tio2 against azole-resistant c. albicans (n = 20), c. glabrata (n = 14) and c. krusei (n = 10) clinical isolates. among tested essential oils, the highest activity has definitely been found ... | 2015 | 25805904 |
| candida infection in the intensive care unit: a study of antifungal susceptibility pattern of candida species in milad hospital, tehran, iran. | the occurrence of candida infections has improved during the past two decades as a result of increase in the number of immunocompromised patients. in this study the antifungal susceptibility patterns of candida species isolated from sterile body sites of patients admitted in milad intensive care unit (icu) during 6 months were determined. | 2015 | 26563641 |
| matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms) and bayesian phylogenetic analysis to characterize candida clinical isolates. | clinical candida isolates from two different hospitals in rome were identified and clustered by maldi-tof ms system and their origin and evolution estimated by bayesian phylogenetic analysis. the different species of candida were correctly identified and clustered separately, confirming the ability of these techniques to discriminate between different candida species. focusing maldi-tof analysis on a single candida species, candida albicans and candida parapsilosis strains clustered differently ... | 2015 | 26551247 |
| epidemiology of invasive candidiasis in a surgical intensive care unit: an observational study. | invasive candidiasis (ic) is a frequent and life-threatening infection in critically ill patients. the aim of this study was to evaluate the epidemiology of ic and the antifungal susceptibility of etiological agents in patients admitted to our surgical intensive care unit (sicu) in spain. | 2015 | 26415526 |
| photodynamic inactivation of a multispecies biofilm using photodithazine(®) and led light after one and three successive applications. | in this investigation, the effectiveness of successive applications of antimicrobial photodynamic inactivation (api) mediated by photodithazine(®) (pdz) and led light was evaluated against a multispecies biofilm formed by candida albicans, candida glabrata, and streptococcus mutans on denture base acrylic resin. standard cell suspensions (bacteria and yeast) were inoculated on acrylic resin samples, and the biofilm was grown for 48 h (37 °c/75 rpm). api was performed by the administration of pdz ... | 2015 | 26404782 |
| enzymatic and hemolytic activity in different candida species. | candida species, in conditions of microbiota imbalance or decreased immune defenses, may be one of the main human fungal pathogens. virulence factors constitute the mechanisms used by the fungus to avoid host defenses. | 2015 | 24704439 |
| in vitro fungicidal activities of anidulafungin, caspofungin, and micafungin against candida glabrata, candida bracarensis, and candida nivariensis evaluated by time-kill studies. | anidulafungin, caspofungin, and micafungin killing activities against candida glabrata, candida bracarensis, and candida nivariensis were evaluated by the time-kill methodology. the concentrations assayed were 0.06, 0.125, and 0.5 μg/ml, which are achieved in serum. anidulafungin and micafungin required between 13 and 26 h to reach the fungicidal endpoint (99.9% killing) against c. glabrata and c. bracarensis. all echinocandins were less active against c. nivariensis. | 2015 | 25801575 |
| candida glabrata, friend and foe. | candida glabratais mostly good, but, at times, it is an opportunistic pathogen. previously known astorulopsis glabrata, it enjoyed a good reputation and was even present in starter cultures. its haploid genome and lack of mating made it an attractive challenge for yeast genetics studies. however, more recently it has become better known due to its character as an emerging cause of candidiasis, and for its resistance to multidrugs that are employed for candidiasis treatment. while now classified ... | 2015 | 29376912 |
| evaluation of the antifungal activities of various extracts from pistacia atlantica desf. | despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple treatment-related side-effects. the present study was designed to investigate the antifungal properties of different extracts from pistacia atlantica desf. | 2015 | 28680993 |
| digestive tract mycobiota: a source of infection. | the human mycobiome includes 390 fungal species detected on the skin, in the vagina, in the oral cavity, and in the digestive tract that includes 335 species and 158 genera. among these, 221 species are found only in the digestive tract, 88 only in the oral cavity, and 26 in both. these species belong to 126 genera of yeast and filamentous fungi, of the ascomycota, basidiomycota, and zygomycota phyla. forty species were identified only by culture, 188 species by molecular techniques, and 19 spec ... | 2015 | 25684583 |
| antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning - in vitro evaluation. | the objective of this in vitro study was to evaluate the antimicrobial action of sodium hypochlorite (0.25% and 0.50%) and 10% castor oil solutions against specific microorganisms, by counting colony forming units (cfu) of clinically important bacteria and candida species. acrylic resin specimens (n = 320; lucitone 550) were obtained from square metal matrices (10 x 10 x 2 mm), sterilized by microwave (650w, for 6 minutes) and contaminated by staphylococcus aureus, pseudomonas aeruginosa, candid ... | 2015 | 26313346 |
| silicon incorporated morpholine antifungals: design, synthesis, and biological evaluation. | known morpholine class antifungals (fenpropimorph, fenpropidin, and amorolfine) were synthetically modified through silicon incorporation to have 15 sila-analogues. twelve sila-analogues exhibited potent antifungal activity against different human fungal pathogens such as candida albicans, candida glabrata, candida tropicalis, cryptococcus neoformans, and aspergillus niger. sila-analogue 24 (fenpropimorph analogue) was the best in our hands, which showed superior fungicidal potential than fenpro ... | 2015 | 26617963 |
| sphingolipids mediate differential echinocandin susceptibility in candida albicans and aspergillus nidulans. | the cell wall synthesis-inhibiting echinocandins, including caspofungin and micafungin, play important roles in the treatment of candidiasis and aspergillosis. previous studies revealed that, in the haploid yeast candida glabrata, sphingolipid biosynthesis pathway mutations confer caspofungin reduced susceptibility (crs) but micafungin increased susceptibility (mis). here, we describe one candida albicans strain (of 10 tested) that similarly yields crs-mis mutants at relatively high frequency. m ... | 2015 | 25824222 |
| cell wall proteome of pathogenic fungi. | a fast development of a wide variety of proteomic techniques supported by mass spectrometry coupled with high performance liquid chromatography has been observed in recent years. it significantly contributes to the progress in research on the cell wall, very important part of the cells of pathogenic fungi. this complicated structure composed of different polysaccharides, proteins, lipids and melanin, plays a key role in interactions with the host during infection. changes in the set of the surfa ... | 2015 | 26192771 |
| update on antifungal drug resistance. | invasive fungal infections remain a major source of global morbidity and mortality, especially among patients with underlying immune suppression. successful patient management requires antifungal therapy. yet, treatment choices are restricted due to limited classes of antifungal agents and the emergence of antifungal drug resistance. in some settings, the evolution of multidrug-resistant strains insensitive to several classes of antifungal agents is a major concern. the resistance mechanisms res ... | 2015 | 26120512 |
| lysophospholipids from the guangxi sponge spirastrella purpurea. | four known (1-4) and two new (5 and 6) lysophospholipids were isolated from the sponge spirastrella purpurea from weizhou island, guangxi autonomous region, china. the structures of the new compounds (5 and 6) were elucidated by detailed spectroscopic techniques, including 1d and 2d nmr ((1)h and (13)c nmr, (1)h-(1)h cosy, hsqc, and hmbc) as well as mass spectrometry and optical rotation experiments. the known compounds (1-4) were identified by comparison of their spectroscopic data and specific ... | 2015 | 26037518 |
| antifungal susceptibilities of candida, cryptococcus neoformans and aspergillus fumigatus from the asia and western pacific region: data from the sentry antifungal surveillance program (2010-2012). | the sentry antifungal surveillance program monitors global susceptibility rates of newer and established antifungal agents. we report the in vitro activity of seven antifungal agents against 496 contemporary clinical isolates of yeasts and molds. the isolates were obtained from 20 laboratories in the asia-western pacific (apac) region during 2010 through 2012. anidulafungin, caspofungin, micafungin, fluconazole, itraconazole, posaconazole and voriconazole were susceptibility tested using clsi me ... | 2015 | 25899126 |
| in vitro activity of 23 tea extractions and epigallocatechin gallate against candida species. | in this study, we investigate the susceptibility of candida albicans, candida glabrata, candida krusei, candida parapsilosis, candida tropicalis, and aspergillus fumigatus using the eucast microdilution minimum inhibitory concentration (mic) method (final tea supernatant concentration range 5.0-0.005 mg/ml) to 23 different teas and tea catechins including epigallocatechin gallate (egcg) isolated from green tea. all teas exhibited potent in vitro antifungal activity against c. glabrata. six out o ... | 2015 | 25605775 |
| possible mechanism of antifungal phenazine-1-carboxamide from pseudomonas sp. against dimorphic fungi benjaminiella poitrasii and human pathogen candida albicans. | investigation of antifungal mechanism of phenazine 1-carboxamide (pc) produced by a pseudomonas strain mcc2142. | 2015 | 25348290 |
| govanoside a, a new steroidal saponin from rhizomes of trillium govanianum. | a new spirostane steroidal saponin, govanoside a (1) along with three known compounds borassoside e (2) pennogenin (3) and diosgenin (4) were isolated from rhizomes of trillium govanianum. their structures were elucidated through 1d, 2d-nmr spectroscopic data analysis and acid hydrolysis. compound (2) in genus trillium and all compounds (1-4) in t. govanianum are reported herein for the first time. furthermore, compounds 1 &2 exhibited good to moderate activities against aspergillus niger atcc 1 ... | 2015 | 26505320 |
| design, synthesis and in vitro bactericidal/fungicidal screening of some vanadyl(iv)complexes with mono- and di-substituted ons donor triazoles. | a new series of anti-bacterial and anti-fungal mono- and di-substituted triazoles (l(1))-(l(6)) have been synthesized and characterized on the basis of their physical, spectral and analytical data. the ligands (l(1))-(l(6)) on reaction with vanadyl(iv) sulphate led to the formation of vanadyl(iv) metal complexes (1)-(4). the structure of the complexes has been established on the basis of their physical, spectral and elemental analyses data. the synthesized ligands and their vanadyl(iv) complexes ... | 2015 | 25716124 |
| maldi-tof mass spectrometry for rapid identification of clinical fungal isolates based on ribosomal protein biomarkers. | this study aimed to evaluate the identification of clinical fungal isolates (yeast and molds) by protein profiling using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof/ms). a total of 125 clinical fungal culture isolates (yeast and filamentous fungi) were collected. the test set included 88 yeast isolates (candida albicans, candida glabrata, candida guilliermondii, candida kefyr, candida krusei, candida parapsilosis, candida rugosa, candida tropicalis and ... | 2015 | 25541362 |
| low-dose irradiation affects the functional behavior of oral microbiota in the context of mucositis. | the role of host-microbe interactions in the pathobiology of oral mucositis is still unclear; therefore, this study aimed to unravel the effect of irradiation on behavioral characteristics of oral microbial species in the context of mucositis. using various experimental in vitro setups, the effects of irradiation on growth and biofilm formation of two candida spp., streptococcus salivarius and klebsiella oxytoca in different culture conditions were evaluated. irradiation did not affect growth of ... | 2015 | 26202372 |
| in vivo biocompatibility and in vitro efficacy of antimicrobial gendine-coated central catheters. | antimicrobial peripherally inserted central catheters (piccs) might reduce the incidence of central line-associated bloodstream infections (clabsi). we tested the biocompatibility of a novel gendine-coated (combination of chlorhexidine [chx] and gentian violet [gv]) picc in a rabbit intravascular model and tested antimicrobial efficacy in comparison with commercially available minocycline/rifampin (m/r)- and chx-treated piccs in an in vitro biofilm colonization model. gendine-coated and uncoated ... | 2015 | 26124171 |
| antiviral, antifungal and antibacterial activities of a bodipy-based photosensitizer. | antimicrobial photodynamic inactivation (apdi) employing the bodipy-based photosensitizer 2,6-diiodo-1,3,5,7-tetramethyl-8-(n-methyl-4-pyridyl)-4,4'-difluoro-boradiazaindacene (dimpy-bodipy) was explored in an in vitro assay against six species of bacteria (eight total strains), three species of yeast, and three viruses as a complementary approach to their current drug-based or non-existent treatments. our best results achieved a noteworthy 5-6 log unit reduction in cfu at 0.1 μm for staphylococ ... | 2015 | 26060922 |
| rate of fks mutations among consecutive candida isolates causing bloodstream infection. | precise fks mutation rates among candida species are undefined because studies have not systematically screened consecutive, disease-causing isolates. the sensititre yeastone (syo) assay measures echinocandin mics against candida with less variability than reference broth microdilution methods. however, clinical breakpoint mics may overstate caspofungin nonsusceptibility compared to other agents. our objectives were to determine candida fks mutation rates by studying consecutive bloodstream isol ... | 2015 | 26392494 |
| peptides from the scorpion vaejovis punctatus with broad antimicrobial activity. | the antimicrobial potential of two new non-disulfide bound peptides, named vpamp1.0 (lpffllslipsaisaikki, amidated) and vpamp2.0 (fwgflgklamkavpsliggnksssk) is here reported. these are 19- and 25-aminoacid-long peptides with +2 and +4 net charges, respectively. their sequences correspond to the predicted mature regions from longer precursors, putatively encoded by cdnas derived from the venom glands of the mexican scorpion vaejovis punctatus. both peptides were chemically synthesized and assayed ... | 2015 | 26352292 |
| draft genome of a commonly misdiagnosed multidrug resistant pathogen candida auris. | candida auris is a multidrug resistant, emerging agent of fungemia in humans. its actual global distribution remains obscure as the current commercial methods of clinical diagnosis misidentify it as c. haemulonii. here we report the first draft genome of c. auris to explore the genomic basis of virulence and unique differences that could be employed for differential diagnosis. | 2015 | 26346253 |
| evaluation of the "dip effect" phenomenon in antifungal susceptibility testing of candida spp. against echinocandins by use of gradient concentration strips. | the "dip effect" phenomenon complicates antifungal susceptibility testing with gradient concentration strips. of 60 candida isolates tested with the three echinocandins, this phenomenon was observed only for caspofungin with most (>90%) candida albicans, candida glabrata, and candida tropicalis isolates and for isolates with clsi mics of ≤0.25 mg/liter. in order to facilitate mic determination, a practical approach was developed using the inhibition zones at 32, 8, 2, and 1 mg/liter, increasing ... | 2015 | 26338855 |
| prevalence and fluconazole susceptibility profile of candida spp. clinical isolates in a brazilian tertiary hospital in minas gerais, brazil. | candidiasis has become an important concern for clinical practice, especially with the increasing incidence of immunocompromised patients. in this scenario, the development resistance to fluconazole presents a challenge for treating these opportunistic infections. the aim of this study was to evaluate some epidemiology features of candida infections in a brazilian university hospital using data, previously unavailable. we observed that 44% of the 93 clinical isolates tested, belonged to candida ... | 2015 | 26312420 |
| yeast microbiota of natural cavities of manatees (trichechus inunguis and trichechus manatus) in brazil and its relevance for animal health and management in captivity. | the aim of this study was to characterize the yeast microbiota of natural cavities of manatees kept in captivity in brazil. sterile swabs from the oral cavity, nostrils, genital opening, and rectum of 50 trichechus inunguis and 26 trichechus manatus were collected. the samples were plated on sabouraud agar with chloramphenicol and incubated at 25 °c for 5 days. the yeasts isolated were phenotypically identified by biochemical and micromorphological tests. overall, 141 strains were isolated, of w ... | 2015 | 26308797 |
| antifungal activity of 14-helical β-peptides against planktonic cells and biofilms of candida species. | candida albicans is the most prevalent cause of fungal infections and treatment is further complicated by the formation of drug resistant biofilms, often on the surfaces of implanted medical devices. in recent years, the incidence of fungal infections by other pathogenic candida species such as c. glabrata, c. parapsilosis and c. tropicalis has increased. amphiphilic, helical β-peptide structural mimetics of natural antimicrobial α-peptides have been shown to exhibit specific planktonic antifung ... | 2015 | 26287212 |
| characterization of cell wall proteins in saccharomyces cerevisiae clinical isolates elucidates hsp150p in virulence. | the budding yeast saccharomyces cerevisiae has recently been described as an emerging opportunistic fungal pathogen. fungal cell wall mannoproteins have been demonstrated to be involved in adhesion to inert surfaces and might be engaged in virulence. in this study, we observed four clinical isolates of s. cerevisiae with relatively hydrophobic cell surfaces. yeast cell wall subproteome was evaluated quantitatively by liquid chromatography/tandem mass spectrometry. we identified totally 25 cell w ... | 2015 | 26270963 |
| synergistic effects of tea catechin epigallocatechin gallate and antimycotics against oral candida species. | epigallocatechin gallate (egcg), the major antimicrobial tea polyphenol, has been reported to inhibit the growth of candida albicans planktonic cells and enhance the antifungal activity of antimycotics. we hypothesised that synergism exists between egcg and conventional antimycotics against biofilms of candida species. | 2015 | 26263544 |
| inpatient ophthalmology consultation for fungemia: prevalence of ocular involvement and necessity of funduscopic screening. | to determine the generalizability of recent data assessing the necessity of ophthalmic consultation for fungemic patients, we examined the prevalence, microbial profile, and treatment of fungal chorioretinitis and endophthalmitis among patients with positive fungal cultures referred for ophthalmologic consultation at a tertiary care medical center. | 2015 | 26235339 |
| sensitive and rapid rt-qpcr quantification of pathogenic candida species in human blood. | for accurate diagnosis and appropriate treatment of candidiasis, we developed a highly sensitive quantitative rt-pcr (rt-qpcr) system for five candida species that have been reported to be the major causes of bloodstream fungal infection (candida albicans, candida glabrata, candida tropicalis, candida parapsilosis, and candida krusei), together with a system for all pathogenic candida species. cells of each fungal species spiked into human peripheral blood (pb) were specifically detected at a lo ... | 2015 | 26232708 |
| synthesis and antifungal evaluation of head-to-head and head-to-tail bisamidine compounds. | herein, we describe the antifungal evaluation of 43 bisamidine compounds, of which 26 are new, having the scaffold [am]-[hetar]-[linker]-[hetar]-[am], in which [am] is a cyclic or acyclic amidine group, [linker] is a benzene, pyridine, pyrimidine, pyrazine ring, or an aliphatic chain of two to four carbon, and [hetar] is a 5,6-bicyclic heterocycle such as indole, benzimidazole, imidazopyridine, benzofuran, or benzothiophene. in the head-to-head series the two [hetar] units are oriented such that ... | 2015 | 26209266 |
| delicate metabolic control and coordinated stress response critically determine antifungal tolerance of candida albicans biofilm persisters. | candida infection has emerged as a critical health care burden worldwide, owing to the formation of robust biofilms against common antifungals. recent evidence shows that multidrug-tolerant persisters critically account for biofilm recalcitrance, but their underlying biological mechanisms are poorly understood. here, we first investigated the phenotypic characteristics of candida biofilm persisters under consecutive harsh treatments of amphotericin b. the prolonged treatments effectively killed ... | 2015 | 26195524 |
| retrospective analysis of mortality and candida isolates of 75 patients with candidemia: a single hospital experience. | the mortality rate for candidemia is approximately 30%-60%. however, prognostic factors in patients with candidemia have not yet been elucidated in detail. the aim of the present study was to analyze prognostic factors for candidemia using the mortality rate and candida isolates of patients with candidemia. seventy-five patients with candidemia were analyzed between january 2007 and december 2013. the main outcome of this study was the 30-day mortality rate after the diagnosis of candidemia. the ... | 2015 | 26185460 |
| effect of tyrosol on adhesion of candida albicans and candida glabrata to acrylic surfaces. | the prevention of adhesion of candida cells to acrylic surfaces can be regarded as an alternative to prevent denture stomatitis. the use of quorum sensing molecules, such as tyrosol, could potentially interfere with the adhesion process. therefore, the aim of this study was to assess the effect of tyrosol on adhesion of single and mixed cultures of candida albicans and candida glabrata to acrylic resin surfaces. tyrosol was diluted in each yeast inoculum (10(7) cells/ml in artificial saliva) at ... | 2015 | 26162470 |
| candida isolates from pregnant women and their antifungal susceptibility in a malaysian tertiary-care hospital. | pregnant women are susceptible to vaginal colonization and infection by yeast. the purpose of the study was to determine the prevalence of candida spp in high vaginal swabs of pregnant women and their antifungal susceptibility. | 2015 | 26150863 |
| competitive interactions between c. albicans, c. glabrata and c. krusei during biofilm formation and development of experimental candidiasis. | in this study, we evaluated the interactions between candida albicans, candida krusei and candida glabrata in mixed infections. initially, these interactions were studied in biofilms formed in vitro. cfu/ml values of c. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of c. albicans reduction when this species was associated with c. glabrata and c. krusei, respectively. after that, we expanded this study for in vivo host models of experimental can ... | 2015 | 26146832 |
| biliary amphotericin b pharmacokinetics and pharmacodynamics in critically ill liver transplant recipients receiving treatment with amphotericin b lipid formulations. | fungal cholangitis is a potentially life-threatening condition. as amphotericin b (amb) has a broad antimycotic spectrum, in this study its biliary penetration and activity was determined in two patients treated with liposomal amb (l-amb) and in one patient receiving amb colloidal dispersion (abcd). biliary and plasma amb levels were quantified by high-performance liquid chromatography after purification by solid-phase extraction. for assessment of biliary amb activity, isolates of candida albic ... | 2015 | 26119497 |
| performance of chromogenic media for candida in rapid presumptive identification of candida species from clinical materials. | in perspective of the worldwide increase in a number of immunocompromised patients, the need for identification of candida species has become a major concern. the development of chromogenic differential media, introduced recently, facilitate rapid speciation. however, it can be employed for routine mycology workup only after an exhaustive evaluation of its benefit and cost effectiveness. this study was undertaken to evaluate the benefit and cost effectiveness of chromogenic media for speciation ... | 2015 | 26109791 |
| incidence and species distribution of candidaemia in asia: a laboratory-based surveillance study. | the epidemiology of candidaemia varies between hospitals and geographic regions. although there are many studies from asia, a large-scale cross-sectional study across asia has not been performed. we conducted a 12-month, laboratory-based surveillance of candidaemia at 25 hospitals from china, hong kong, india, singapore, taiwan and thailand. the incidence and species distribution of candidaemia were determined. there were 1601 episodes of candidaemia among 1.2 million discharges. the overall inc ... | 2015 | 26100373 |
| phenotypic detection of genitourinary candidiasis among sexually transmitted disease clinic attendees in ladoke akintola university teaching hospital, osogbo, nigeria. | the management of genitourinary candidiasis (gc) is fraught with challenges, especially, in an era of increasing antifungal resistance. this descriptive cross-sectional study conducted between may 2013 and january 2014 determined the prevalence and characteristics of gc and the species of candida among 369 attendees of a sexually transmitted disease (std) clinic of ladoke akintola university teaching hospital, osogbo, nigeria. appropriate urogenital specimen collected from each attendee was exam ... | 2015 | 26064140 |
| antifungal activity of extracts and isolated compounds from buchenavia tomentosa on candida albicans and non-albicans. | this study aimed to evaluate the antifungal activity of buchenavia tomentosa extract and bioactive compounds on six candida species. | 2015 | 26059616 |