Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| comments on "candida glabrata candidemia; an emerging threat in critically ill patients". | 2015 | 25983444 | |
| tipping the balance both ways: drug resistance and virulence in candida glabrata. | among existing fungal pathogens, candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. moreover, resistance to the class of echinocandins, which was introduced in the late 1990s, is rising in several institutions. the recent emergence of isolates with acquired resistance to both classes of agents i ... | 2015 | 25979690 |
| anti-vancomycin-resistant enterococcus faecium and e. faecalis activities of (-)-gossypol and derivatives from thespesia garckeana. | the root extract of thespesia garckeana yielded three known oxidatively coupled sesquiterpenoids, namely (-)-gossypol (1) and two of its derivatives (-)-6-methoxygossypol (2) and (+)-6,6'-dimethoxygossypol (3), and the stem bark afforded (e)-docosyl-3-(3,4-dihydroxyphenyl) acrylate (4), stigmasterol (5) and betulinic acid (6). the structures of the isolated compounds were determined on the basis of full spectral data (1d and 2d nmr and hrms) and comparison with literature values. compound 1 show ... | 2015 | 25973489 |
| stress response and expression of fluconazole resistance associated genes in the pathogenic yeast candida glabrata deleted in the cgpdr16 gene. | in yeasts, the pdr16 gene encodes a phosphatidylinositol transfer protein which belongs to the sec14 homologue (sfh) family and localizes to lipid droplets, microsomes and at the cell periphery. the loss of its function alters the lipid droplet metabolism and plasma membrane properties, and renders yeast cells more sensitive to azole antimycotics. in this study, the entire chromosomal cgpdr16 orf was replaced by the scura3 gene both in azole sensitive and azole resistant strains of candida glabr ... | 2015 | 25946325 |
| induction of ros generation by fluconazole in candida glabrata: activation of antioxidant enzymes and oxidative dna damage. | in this study, we assessed the generation of reactive oxygen species (ros) induced by subinhibitory concentration of fluconazole in susceptible and resistant candida glabrata strains at stationary growth phase and measured their oxidative responses parameters: glutathione peroxidase (gpx), superoxide dismutase (sod), glutathione-s-transferase (gst), consumption of hydrogen peroxide, and total glutathione, as well as oxidative damage in lipids, proteins, and dna. data showed that fluconazole incr ... | 2015 | 25935629 |
| exogenous folates stimulate growth and budding of candida glabrata. | folate, vitamin b9, is well recognized as being essential for cell growth. the utilization of folate is common to all cells, but the source of it may be quite different. for example, mammalian cells depend on exogenous uptake of folates, while plants and microbes can synthesize them. there has been little consideration of uptake of folate in microbial cells, and studies on the effects of folates in mammalian cells, where conditions are restricted. this study shows that exogenous folates (folic a ... | 2015 | 28357288 |
| antifungal activity of ellagic acid in vitro and in vivo. | ellagic acid (ea) has been shown to have antioxidant, antibacterial, and anti-inflammatory activities. in uighur traditional medicine, euphorbia humifusa willd is used to treat fungal diseases, and recent studies suggest that it is the ea content which is responsible for its therapeutic effect. however, the effects of ea on antifungal activity have not yet been reported. this study aimed to investigate the inhibitory effect of ea on fungal strains both in vitro and in vivo. the minimal inhibitor ... | 2015 | 25919446 |
| phytase-producing capacity of yeasts isolated from traditional african fermented food products and phypk gene expression of pichia kudriavzevii strains. | phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. ninety-three yeast isolates from traditional african fermented food products, belonging to nine species (pichia kudriavzevii, saccharomyces cerevisiae, clavispora lusitaniae, kluyveromyces marxianus, millerozyma farinosa, candida glabrata, wickerhamomyces anomalus, hanseniaspora guilliermondii and debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were a ... | 2015 | 25910031 |
| candida glabrata fungaemia at an australian cancer centre: epidemiology, risk factors and therapy. | 2015 | 25907425 | |
| the ever-evolving landscape of candidaemia in patients with acute leukaemia: non-susceptibility to caspofungin and multidrug resistance are associated with increased mortality. | the epidemiology and clinical course of candidaemia in patients with acute leukaemia, a population frequently exposed to antifungals, have not been extensively studied. in the present contemporary series of acute leukaemia patients, we describe patient characteristics, candida species and mic distributions and investigate the association between antifungal resistance and all-cause mortality. | 2015 | 25855759 |
| maldi-tof typing highlights geographical and fluconazole resistance clusters in candida glabrata. | utilizing matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectra for candida glabrata typing would be a cost-effective and easy-to-use alternative to classical dna-based typing methods. this study aimed to use maldi-tof for the typing of c. glabrata clinical isolates from various geographical origins and test its capacity to differentiate between fluconazole-sensitive and -resistant strains.both microsatellite length polymorphism (mlp) and maldi-tof mass spectra of 5 ... | 2015 | 25841053 |
| early-onset candida glabrata interface keratitis after deep anterior lamellar keratoplasty. | fungal interface keratitis by candida species can occur several weeks to several months after deep anterior lamellar keratoplasty (dalk). here, we report a case of early-onset fungal interface keratitis by candida glabrata after dalk. | 2015 | 25822017 |
| candida glabrata candidemia: an emerging threat in critically ill patients. | candidemia is an important nosocomial blood stream infection in critically ill patients. although several studies have addressed candidemia, very few have reviewed the impact of candida glabrata candidemia in intensive care unit (icu) patients. | 2015 | 25810610 |
| candida glabrata candidemia. | 2015 | 25810607 | |
| significantly improved thermostability of a reductase cgkr1 from candida glabrata with a key mutation at asp 138 for enhancing bioreduction of aromatic α-keto esters. | the keto ester reductase from candida glabrata, designated as cgkr1, is a highly versatile biocatalyst with a broad substrate spectrum. its substrate preference was altered by rational design of the active pocket for bioreduction of aromatic α-keto esters in our previous work. however, its practical application is still hindered by its poor thermostability and high biocatalyst loading. in this work, random mutagenesis followed by a saturation mutagenesis was performed aiming to improve its therm ... | 2015 | 25795440 |
| of mice, flies--and men? comparing fungal infection models for large-scale screening efforts. | studying infectious diseases requires suitable hosts for experimental in vivo infections. recent years have seen the advent of many alternatives to murine infection models. however, the use of non-mammalian models is still controversial because it is often unclear how well findings from these systems predict virulence potential in humans or other mammals. here, we compare the commonly used models, fruit fly and mouse (representing invertebrate and mammalian hosts), for their similarities and deg ... | 2015 | 25786415 |
| the mitogen-activated protein kinase cghog1 is required for iron homeostasis, adherence and virulence in candida glabrata. | candida glabrata has emerged as a major fungal pathogen over the last two decades, although our understanding of its survival strategies inside the mammalian host remains rudimentary. an important requirement for survival in vivo is the ability to acquire critical nutrients such as iron from host niches of varied iron content. in the present study, we demonstrate for the first time that c. glabrata cells respond to high external iron levels via activation of two stress-responsive mitogen-activat ... | 2015 | 25772226 |
| structural and mechanistic analysis of the slx1-slx4 endonuclease. | the slx1-slx4 endonuclease required for homologous recombination and dna repair in eukaryotic cells cleaves a variety of branched dna structures. the nuclease subunit slx1 is activated by association with a scaffolding protein slx4. at the present time, little is known about the structure of slx1-slx4 or its mechanism of action. here, we report the structural insights into slx1-slx4 by detailing the crystal structure of candida glabrata (cg) slx1 alone and in combination with the c-terminal regi ... | 2015 | 25753413 |
| avoiding the ends: internal epitope tagging of proteins using transposon tn7. | peptide tags fused to proteins are used in a variety of applications, including as affinity tags for purification, epitope tags for immunodetection, or fluorescent protein tags for visualization. however, the peptide tags can disrupt the target protein function. when function is disrupted by fusing a peptide to either the n or c terminus of the protein of interest, identifying alternative ways to create functional tagged fusion proteins can be difficult. here, we describe a method to introduce p ... | 2015 | 25745023 |
| yap7 is a transcriptional repressor of nitric oxide oxidase in yeasts, which arose from neofunctionalization after whole genome duplication. | flavohemoglobins are the main detoxifiers of nitric oxide (no) in bacteria and fungi and are induced in response to nitrosative stress. in fungi, the flavohemoglobin encoding gene yhb1 is positively regulated by transcription factors which are activated upon no exposure. in this study, we show that in the model yeast saccharomyces cerevisiae and in the human pathogen candida glabrata, the transcription factor yap7 constitutively represses yhb1 by binding its promoter. consequently, yap7 deletion ... | 2015 | 25732006 |
| mitochondrial engineering of the tca cycle for fumarate production. | microbial fumarate production from renewable feedstock is a promising and sustainable alternative to petroleum-based chemical synthesis. here, mitochondrial engineering was used to construct the oxidative pathway for fumarate production starting from the tca cycle intermediate α-ketoglutarate in candida glabrata. accordingly, α-ketoglutarate dehydrogenase complex (kgd), succinyl-coa synthetase (suclg), and succinate dehydrogenase (sdh) were selected to be manipulated for strengthening the oxidat ... | 2015 | 25708514 |
| terminal-stage prognostic analysis in candidemia. | candidemia has an extremely high mortality rate but is not always the direct cause of death. therefore, determining the effect of candidemia on death is extremely difficult. | 2015 | 25697387 |
| [evaluation of the rapid trehalose test for the identification of the candida glabrata]. | to explore a rapid and cost-effective method for identification of candida glabrata through the comparison of two different methods, using molecular methods of sequencing as gold standard. | 2015 | 25686354 |
| early initiation of appropriate treatment is associated with increased survival in cancer patients with candida glabrata fungaemia: a potential benefit from infectious disease consultation. | in patients with malignancies, candida glabrata is one of the most frequent non-albicans candida clinical isolates. as antifungal resistance in c. glabrata is common, we investigated the relationship between early appropriate antifungal treatment, infectious disease (id) consultation and mortality in a contemporary cohort of cancer patients with c. glabrata fungaemia. we included patients with at least one c. glabrata-positive blood culture and symptoms or signs of infection seen at the md ander ... | 2015 | 25636931 |
| force nanoscopy of hydrophobic interactions in the fungal pathogen candida glabrata. | candida glabrata is an opportunistic human fungal pathogen which binds to surfaces mainly through the epa family of cell adhesion proteins. while some epa proteins mediate specific lectin-like interactions with human epithelial cells, others promote adhesion and biofilm formation on plastic surfaces via nonspecific interactions that are not yet elucidated. we report the measurement of hydrophobic forces engaged in epa6-mediated cell adhesion by means of atomic force microscopy (afm). using singl ... | 2015 | 25621738 |
| determination of micing: a new assay for assessing minimal inhibitory concentration for invasive growth. | our work was focused on a new assay for characterising clinically important yeast. this assay was developed due to the need for new diagnostic methods for recognising potentially virulent strains of increasingly important non-albicans yeast pathogens, such as saccharomyces cerevisiae and candida glabrata. with the great diversity among strains for virulence and virulence factors, identification to the species level is not sufficient; therefore, testing for specific virulent traits remains the be ... | 2015 | 25620781 |
| a novel candida glabrata cell wall associated serine protease. | we set out to identify the candida glabrata cell wall attached proteases which may play a role as virulence factors in candidosis, particularly in the immunocompromized host. we studied a clinical c. glabrata strain t-1639, which was isolated from a patient from the helsinki university central hospital. with non-reducing 2-d electrophoresis using parallel fluorogenic gels and mass spectrometry we identified a novel appr. 25 kda (192 aa in length) cell wall located protease with an estimated pi o ... | 2015 | 25617734 |
| detection and quantification of fluconazole within candida glabrata biofilms. | candida infections are often associated with biofilms and consequent high resistance to most common drugs (e.g. azoles). these resistance mechanisms are not only associated with the biofilm yeast physiology, but also with the presence of a diffusional barrier imposed by the biofilm matrix; however, the real biochemical role of the biofilm components remains very unclear. so, in order to further clarify this issue, we intend to determine, for the first time, fluconazole in biofilms within both su ... | 2015 | 25614085 |
| candida glabrata and candida tropicalis in an immunocompetent patient: a case report. | to report a case of candida glabrata and tropicalis pneumonia in an immunocompetent patient. | 2015 | 25613052 |
| the epa2 adhesin encoding gene is responsive to oxidative stress in the opportunistic fungal pathogen candida glabrata. | candida glabrata has emerged as an important opportunistic pathogen in both mucosal and bloodstream infections. c. glabrata contains 67 adhesin-like glycosylphosphatidylinositol-cell-wall proteins (gpi-cwps), which are classified into seven groups and the largest is the epa family. epa proteins are very diverse and their expression is differentially regulated. like many of the epa genes, epa2 is localized in a subtelomeric region where it is subject to chromatin-based transcriptional silencing a ... | 2015 | 25586543 |
| in vitro evaluation of the acquisition of resistance, antifungal activity and synergism of brazilian red propolis with antifungal drugs on candida spp. | to evaluate the ability of candida parapsilosis and candida glabrata to develop phenotypic resistance to a benzophenone enriched fraction obtained from brazilian red propolis (bzp-brp) as compared to fluconazole (flc). to investigate possible synergy between bzp-brp and flc and anidulafungin (and). | 2015 | 25565139 |
| emergence of multiple resistance profiles involving azoles, echinocandins and amphotericin b in candida glabrata isolates from a neutropenia patient with prolonged fungaemia. | 2015 | 25550394 | |
| changing epidemiology of candidemia in a medical center in middle taiwan. | candidemia remains a major cause of morbidity and mortality in the health care setting, and the epidemiology of candida infection is changing. | 2015 | 24113067 |
| candidemia in intensive care unit patients: a one year study from a tertiary care center in south india. | the occurrence of candidemia is on a rise worldwide. non-albicans candida species have emerged as major causes of candidemia in many countries. added to it is the problem of antifungal resistance in candida isolates. | 2015 | 24029196 |
| enzymatic and hemolytic activity in different candida species. | candida species, in conditions of microbiota imbalance or decreased immune defenses, may be one of the main human fungal pathogens. virulence factors constitute the mechanisms used by the fungus to avoid host defenses. | 2015 | 24704439 |
| in vivo candida glabrata biofilm development on foreign bodies in a rat subcutaneous model. | biofilm studies have been mostly dedicated to the major human fungal pathogen candida albicans, whereas much less is known about this virulence factor in candida glabrata, certainly under in vivo conditions. this study provides a deeper understanding of the biofilm development of c. glabrata, its architecture and susceptibility profile to fluconazole and echinocandins. | 2015 | 25406296 |
| in vitro anti-yeast activity of chloramphenicol: a preliminary report. | chloramphenicol is a bacteriostatic antimicrobial agent but its antifungal activity is not known. the present study aimed to investigate the activity of chloramphenicol against 30 representative yeasts. the antimicrobial assay of chloramphenicol (50mg/ml; 100mg/ml and 200mg/ml) was determined by the disc diffusion method using mueller-hinton agar against 30 representative yeast strains. zone of inhibition was read after 48-72h incubation at 37°c and results were compared with some standard antif ... | 2015 | 25497707 |
| epidemiology of invasive candidiasis in a surgical intensive care unit: an observational study. | invasive candidiasis (ic) is a frequent and life-threatening infection in critically ill patients. the aim of this study was to evaluate the epidemiology of ic and the antifungal susceptibility of etiological agents in patients admitted to our surgical intensive care unit (sicu) in spain. | 2015 | 26415526 |
| photodynamic inactivation of a multispecies biofilm using photodithazine(®) and led light after one and three successive applications. | in this investigation, the effectiveness of successive applications of antimicrobial photodynamic inactivation (api) mediated by photodithazine(®) (pdz) and led light was evaluated against a multispecies biofilm formed by candida albicans, candida glabrata, and streptococcus mutans on denture base acrylic resin. standard cell suspensions (bacteria and yeast) were inoculated on acrylic resin samples, and the biofilm was grown for 48 h (37 °c/75 rpm). api was performed by the administration of pdz ... | 2015 | 26404782 |
| surveillance of antifungal susceptibilities in clinical isolates of candida species at 36 hospitals in china from 2009 to 2013. | the purpose of this study was to determine the species distribution and to monitor the antifungal susceptibility profiles of clinical candida isolates collected in china from 2009 to 2013. | 2015 | 25541294 |
| species spectrum and antifungal susceptibility profile of vaginal isolates of candida in kuwait. | the study was undertaken to determine the prevalence of vulvovaginal candidiasis (vvc) among patients with vaginitis, frequency of different candida species, and their susceptibility profile. | 2015 | 25534676 |
| lab-scale preparations of candida albicans and dual candida albicans-candida glabrata biofilms on the surface of medical-grade polyvinyl chloride (pvc) perfusion tube using a modified gravity-supported free-flow biofilm incubator (gs-ffbi). | the assembly of a man-made gravity-supported free-flow biofilm incubator (gs-ffbi) was described, which was composed of a gas cushion injector and four incubators. the gs-ffbi had the characteristics of (i) a bottom-up flow direction, and (ii) lab-scale biofilm preparation without the use of a multichannel pump. two opportunistic fungal strains, namely candida albicans and candida glabrata, were employed to incubate c. albicans and dual c. albicans-c. glabrata biofilms on the surface of medical- ... | 2015 | 25528294 |
| lupin peptone as a replacement for animal-derived peptone in rich culture media for yeast. | lupin peptone was shown to be a suitable replacement for traditional bacteriological peptone in the culture of candida glabrata, candida albicans and saccharomyces cerevisiae. this new medium formulation allows yeast researchers to increase safety and to eliminate the use of animal products for the culture of yeast in rich medium. | 2015 | 25514068 |
| effects of bovine lactoferrin to oral candida albicans and candida glabrata isolates recovered from the saliva in elderly people. | the effects of bovine lactoferrin (blf) on the growth of candida species and on inflammatory cytokine production in gingival keratinocytes, ndusd-1 co-cultured with candida strains were investigated. the results showed that blf at 10 and 100 lg/ml significantly inhibits the growth of two c. albicans strains and two c. glabrata strains isolated from the saliva of elderly people requiring nursing care, respectively. the levels of inflammatory cytokines, interleukin (il)-6, and il-8 in ndusd-1 cocu ... | 2015 | 25756168 |
| [fungal infections and ureteral material: how to manage?]. | urinary tract infections due to candida species are mostly encountered in hospital environment. the management of candiduria on ureteral catheter is not consensual. the objective of our work was to make a review of medical literature related to definition, physiopathology, management and prevention of candiduria on ureteral catheter. | 2015 | 25724861 |
| guideline: vulvovaginal candidosis (awmf 015/072), s2k (excluding chronic mucocutaneous candidosis). | the oestrogenised vagina is colonised by candida species in at least 20% of women; in late pregnancy and in immunosuppressed patients, this increases to at least 30%. in most cases, candida albicans is involved. host factors, particularly local defence mechanisms, gene polymorphisms, allergies, serum glucose levels, antibiotics, psycho-social stress and oestrogens influence the risk of candidal vulvovaginitis. non-albicans species, particularly candida glabrata, and in rare cases also saccharomy ... | 2015 | 25711406 |
| host factors and clinical outcomes of candida colonization in critically ill patients. | this study aimed to describe factors and outcomes associated with candida colonization of critically ill patients. this was a cross-sectional study conducted over 2 weeks in the intensive care unit (icu) of a tertiary care hospital at the texas medical center, houston, tx. all candida samples were prospectively collected with demographic and clinical data collected retrospectively. we examined 48 patients, 32 (67%) were colonized with candida spp; 25 (52.1%) patients were isolated with candida a ... | 2015 | 25173925 |
| susceptibility of candida albicans and candida glabrata biofilms to silver nanoparticles in intermediate and mature development phases. | the aim of this study was to investigate the susceptibility of candida albicans and candida glabrata biofilm development, in their intermediate and maturation stages, to the influence of silver nanoparticles (sn). | 2015 | 25168655 |
| simultaneous detection of four common oral candida species from blood samples by the fluorescence polarization assay. | the genus candida is both the commensal microbe and the opportunistic pathogen, containing approximately 200 species inhabiting in oral cavity of 53 % of the general population. candida species can cause the diseases from local mucosal infections to systemic mycoses, even life-threatening infections in immunocompromised individuals. the timely differentiation of candida species is important for the guidance of clinical medication. four common candida species in chinese population (candida albica ... | 2015 | 25308858 |
| antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning - in vitro evaluation. | the objective of this in vitro study was to evaluate the antimicrobial action of sodium hypochlorite (0.25% and 0.50%) and 10% castor oil solutions against specific microorganisms, by counting colony forming units (cfu) of clinically important bacteria and candida species. acrylic resin specimens (n = 320; lucitone 550) were obtained from square metal matrices (10 x 10 x 2 mm), sterilized by microwave (650w, for 6 minutes) and contaminated by staphylococcus aureus, pseudomonas aeruginosa, candid ... | 2015 | 26313346 |
| inhibitory activity in vitro of probiotic lactobacilli against oral candida under different fermentation conditions. | clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral candida species. sugar utilisation by both lactobacilli and candida was also assessed. agar overlay assay was utilised to study growth inhibition of candida albicans, candida glabrata and candida krusei by lactobacillus rhamnosus gg, ... | 2015 | 25380800 |
| oral candida carriage and prevalence of candida species among maras powder users and non-users. | the aim of this study was to investigate candida carriage and species in maras powder users and non-users. | 2015 | 25213478 |
| in vitro fungicidal activities of anidulafungin, caspofungin, and micafungin against candida glabrata, candida bracarensis, and candida nivariensis evaluated by time-kill studies. | anidulafungin, caspofungin, and micafungin killing activities against candida glabrata, candida bracarensis, and candida nivariensis were evaluated by the time-kill methodology. the concentrations assayed were 0.06, 0.125, and 0.5 μg/ml, which are achieved in serum. anidulafungin and micafungin required between 13 and 26 h to reach the fungicidal endpoint (99.9% killing) against c. glabrata and c. bracarensis. all echinocandins were less active against c. nivariensis. | 2015 | 25801575 |
| phylogenetic and transcripts profiling of glucose sensing related genes in candida glabrata. | the sensing mechanism of glucose in saccharomyces cerevisiae is well studied. however, such information is scarcely found in other yeast species such as candida glabrata. | 2015 | 26855740 |
| candida glabrata renal abscesses in a peritoneal dialysis patient. | 2015 | 22302932 | |
| an essential role for phosphatidylinositol 3-kinase in the inhibition of phagosomal maturation, intracellular survival and virulence in candida glabrata. | the yeast class iii phosphoinositide 3-kinase (pi3k) that catalyses production of the lipid signalling molecule, phosphatidylinositol-3-phosphate, is primarily implicated in vesicle-mediated transport and autophagy. in this study, we identified, through a genetic screen, the candida glabrata cgvps15 gene, an orthologue of the saccharomyces cerevisiae pi3k regulatory subunit-encoding open reading frame (orf) to be required for impairment of phagosomal maturation in human macrophages. we also disr ... | 2015 | 25223215 |
| evaluation of the antifungal activities of various extracts from pistacia atlantica desf. | despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple treatment-related side-effects. the present study was designed to investigate the antifungal properties of different extracts from pistacia atlantica desf. | 2015 | 28680993 |
| candida glabrata, friend and foe. | candida glabratais mostly good, but, at times, it is an opportunistic pathogen. previously known astorulopsis glabrata, it enjoyed a good reputation and was even present in starter cultures. its haploid genome and lack of mating made it an attractive challenge for yeast genetics studies. however, more recently it has become better known due to its character as an emerging cause of candidiasis, and for its resistance to multidrugs that are employed for candidiasis treatment. while now classified ... | 2015 | 29376912 |
| declining incidence of candidemia and the shifting epidemiology of candida resistance in two us metropolitan areas, 2008-2013: results from population-based surveillance. | recent reports have demonstrated a decline in bacterial bloodstream infections (bsis) following adherence to central line insertion practices; however, declines have been less evident for bsis due to candida species. | 2015 | 25822249 |
| mechanisms of azole resistance among clinical isolates of candida glabrata in poland. | candida glabrata is currently ranked as the second most frequently isolated aetiological agent of human fungal infections, next only to candida albicans. in comparison with c. albicans, c. glabrata shows lower susceptibility to azoles, the most common agents used in treatment of fungal infections. interestingly, the mechanisms of resistance to azole agents in c. albicans have been much better investigated than those in c. glabrata. the aim of the presented study was to determine the mechanisms o ... | 2015 | 25818698 |
| essential oils, silver nanoparticles and propolis as alternative agents against fluconazole resistant candida albicans, candida glabrata and candida krusei clinical isolates. | development of effective and safe therapeutic treatment of fungal infections remains one of the major challenge for modern medicine. the aim of presented investigation was to analyze the in vitro antifungal activity of selected essential oils, ethanolic extracts of propolis and silver nanoparticles dropped on tio2 against azole-resistant c. albicans (n = 20), c. glabrata (n = 14) and c. krusei (n = 10) clinical isolates. among tested essential oils, the highest activity has definitely been found ... | 2015 | 25805904 |
| antimicrobial potential of 27 plants consumed by chimpanzees (pan troglodytes verus blumenbach) in ivory coast. | due to their genetic proximity, chimpanzees share with human several diseases including bacterial, fungal and viral infections, such as candidiasis, acquired immune deficiency syndrome (aids), ebola virus disease. however, in its natural environment, chimpanzees are tolerant to several pathogens including simian immunodeficiency virus (siv), virus related to human immunodeficiency virus (hiv) that contribute to the emergence of opportunistic diseases such as microbial infections. | 2015 | 26498034 |
| efficient mating-type switching in candida glabrata induces cell death. | candida glabrata is an apparently asexual haploid yeast that is phylogenetically closer to saccharomyces cerevisiae than to candida albicans. its genome contains three mat-like cassettes, mat, which encodes either mata or matalpha information in different strains, and the additional loci, hml and hmr. the genome also contains an ho gene homolog, but this yeast has never been shown to switch mating-types spontaneously, as s. cerevisiae does. we have recently sequenced the genomes of the five spec ... | 2015 | 26491872 |
| assessment of echinocandin regimens by pharmacokinetic/pharmacodynamic analysis against candida spp. in paediatric patients. | this study aimed to investigate the cumulative fraction of response of various echinocandin (caspofungin, micafungin and anidulafungin) dosing regimens against candida spp. in paediatric patients with invasive fungal infections (ifis). monte carlo simulations were performed using previously published pharmacokinetic parameters and pharmacodynamic data to evaluate the ability of each echinocandin regimen in terms of fauc/mic (free drug area under the concentration-time curve/minimum inhibition co ... | 2015 | 26602452 |
| candida infection in the intensive care unit: a study of antifungal susceptibility pattern of candida species in milad hospital, tehran, iran. | the occurrence of candida infections has improved during the past two decades as a result of increase in the number of immunocompromised patients. in this study the antifungal susceptibility patterns of candida species isolated from sterile body sites of patients admitted in milad intensive care unit (icu) during 6 months were determined. | 2015 | 26563641 |
| matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms) and bayesian phylogenetic analysis to characterize candida clinical isolates. | clinical candida isolates from two different hospitals in rome were identified and clustered by maldi-tof ms system and their origin and evolution estimated by bayesian phylogenetic analysis. the different species of candida were correctly identified and clustered separately, confirming the ability of these techniques to discriminate between different candida species. focusing maldi-tof analysis on a single candida species, candida albicans and candida parapsilosis strains clustered differently ... | 2015 | 26551247 |
| study of clinical spectrum and risk factors of neonatal candidemia. | candida colonization in neonates results in significant morbidity and mortality. the prevalence and clinical significance of neonatal candidemia are poorly understood. | 2015 | 26549069 |
| clinical perspectives on echinocandin resistance among candida species. | we review and offer our clinical perspectives on the emergence of echinocandin-resistant candida. | 2015 | 26524326 |
| inherited card9 deficiency in otherwise healthy children and adults with candida species-induced meningoencephalitis, colitis, or both. | invasive infections of the central nervous system (cns) or digestive tract caused by commensal fungi of the genus candida are rare and life-threatening. the known risk factors include acquired and inherited immunodeficiencies, with patients often displaying a history of multiple infections. cases of meningoencephalitis, colitis, or both caused by candida species remain unexplained. | 2015 | 25702837 |
| probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing candida glabrata isolates. | this study investigates the antagonistic effects of the probiotic strains lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 against vulvovaginal candidiasis (vvc)-causing candida glabrata. | 2015 | 25688886 |
| synthesis, qsar and anticandidal evaluation of 1,2,3-triazoles derived from naturally bioactive scaffolds. | in the present study, we used eight natural precursors (1a-h) with most of them having promising antimicrobial activities and synthesised their novel 1,2,3-triazole derivatives (3a-h). in the reaction sequences, the precursor compounds (1a-h) were converted to their respective alkyne (2a-h) followed by addition of benzyl azide freshly prepared by the reaction of benzyl bromide with sodium azide using [3 + 2] azide-alkyne cycloaddition strategy. structural elucidation of all the triazole derivati ... | 2015 | 25686593 |
| the effect of antifungal combination on transcripts of a subset of drug-resistance genes in clinical isolates of candida species induced biofilms. | biofilm formation is often associated with increased candida resistance toward antifungal agents. therefore, the current study aimed to assess the incidence of biofilm formation among candida isolates and to investigate the effect of high doses of fluconazole {flc}, voriconazole {voc} and amphotericin b {amb}, singly and in combination on mature biofilms. moreover, it aimed to assess the expression of selected genes (cdr1, kre1 and skn1) responsible for candida biofilm resistance. the study incl ... | 2015 | 25685044 |
| patterns of candida biofilm on intrauterine devices. | biofilms are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. we aimed to screen and characterize biofilm formation by different isolates of candida on removed intrauterine devices (iuds), to perform experimental biofilm formation with isolated strains, and to examine biofilm by the crystal violet and xtt reduction assays and scann ... | 2015 | 25681320 |
| site-specific mesenchymal control of inflammatory pain to yeast challenge in vulvodynia-afflicted and pain-free women. | fibroblast strains were derived from 2 regions of the lower genital tract of localized provoked vulvodynia (lpv) cases and pain-free controls. sixteen strains were derived from 4 cases and 4 controls, age and race matched, after presampling mechanical pain threshold assessments. strains were challenged with 6 separate stimuli: live yeast species (candida albicans, candida glabrata, candida tropicalis, and saccharomyces cerevisiae), yeast extract (zymosan), or inactive vehicle. production of pros ... | 2015 | 25679469 |
| design of micelle nanocontainers based on pdmaema-b-pcl-b-pdmaema triblock copolymers for the encapsulation of amphotericin b. | the clinical application of amphotericin b (amb), a broad spectrum antifungal agent, is limited by its poor solubility in aqueous medium and also by its proven renal toxicity. in this work, amb was encapsulated in micelles obtained from the self-assembly of pdmaema-b-pcl-b-pdmaema triblock copolymers. the amount of encapsulated amb depended on the copolymer composition, and short blocks of polycaprolactone (pcl) and poly(2-dimethylaminoethyl methacrylate) (pdmaema) showed better performance. all ... | 2015 | 25669917 |
| current methods for identifying clinically important cryptic candida species. | in recent years, the taxonomy of the most important pathogenic candida species (candida albicans, candida parapsilosis and candida glabrata) has undergone profound changes due to the description of new closely-related species. this has resulted in the establishment of cryptic species complexes difficult to recognize in clinical diagnostic laboratories. the identification of these novel candida species seems to be clinically relevant because it is likely that they differ in virulence and drug res ... | 2015 | 25659326 |
| susceptibility characterisation of candida spp. to four essential oils. | in the present investigation, anti-candida activity of four essential oils i.e. black cumin (nigella sativa), curry leaf (murraya koienigii), ajwain (trachiyspirum ammi), and betel leaf (piper betel) were screened against four human pathogenic species of candida viz. candida albicans, candida tropicalis, candida glabrata, and candida parapsilosis. the minimum inhibitory concentration (mic) values of the oils ranged between 15.62 and 250 μl/ml while studied through tube dilution method. the oils ... | 2015 | 25657164 |
| invasive candida infections in surgical patients in intensive care units: a prospective, multicentre survey initiated by the european confederation of medical mycology (ecmm) (2006-2008). | a prospective, observational, multicentre study of invasive candidosis (ic) in surgical patients in intensive care units (icus) was conducted from 2006 to 2008 in 72 icus in 14 european countries. a total of 779 patients (62.5% males, median age 63 years) with ic were included. the median rate of candidaemia was 9 per 1000 admissions. in 10.8% the infection was already present at the time of admission to icu. candida albicans accounted for 54% of the isolates, followed by candida parapsilosis 18 ... | 2015 | 25636940 |
| analysis of the essentiality of rom2 genes in the pathogenic yeasts candida glabrata and candida albicans using temperature-sensitive mutants. | to analyse the essentiality of the rom2 genes originating from the pathogenic yeasts candida glabrata and candida albicans by using temperature-sensitive (ts) mutants. | 2015 | 25604069 |
| candida albicans opi1 regulates filamentous growth and virulence in vaginal infections, but not inositol biosynthesis. | scopi1p is a well-characterized transcriptional repressor and master regulator of inositol and phospholipid biosynthetic genes in the baker's yeast saccharomyces cerevisiae. an ortholog has been shown to perform a similar function in the pathogenic fungus candida glabrata, but with the distinction that cgopi1p is essential for growth in this organism. however, in the more distantly related yeast yarrowia lipolytica, the opi1 homolog was not found to regulate inositol biosynthesis, but alkane oxi ... | 2015 | 25602740 |
| in vitro activity of a hos2 deacetylase inhibitor, mgcd290, in combination with echinocandins against echinocandin-resistant candida species. | mgcd290, a hos2 fungal histone deacetylase inhibitor, showed modest activity when tested alone (mic range, 0.12-4 μg/ml; mic50/90, 0.5/4 μg/ml) against candida glabrata (n=15; 14 fks mutants; 5 also fluconazole resistant), candida albicans (8 fks mutants; 2 also fluconazole resistant), candida tropicalis (4 fks mutants), and candida krusei (3 fks mutants). however, mgcd290 showed synergy or partial synergy for 33.3%, 30.1%, 36.7%, and 80.0% of the isolates when tested with anidulafungin, caspofu ... | 2015 | 25600842 |
| molecular identification and antifungal susceptibility of 186 candida isolates from vulvovaginal candidiasis in southern china. | there is limited information regarding the molecular epidemiology and antifungal susceptibilities of candida isolates using the neo-sensitabs method in patients with vulvovaginal candidiasis (vvc). from august 2012 to march 2013, 301 non-pregnant patients aged 18-50 years with suspected vvc were prospectively screened at a teaching hospital in southern china. the vaginal isolates were identified by dna sequencing of internal transcribed spacer and the d1/d2 domain. antifungal susceptibility test ... | 2015 | 25596116 |
| t2 magnetic resonance assay for the rapid diagnosis of candidemia in whole blood: a clinical trial. | microbiologic cultures, the current gold standard diagnostic method for invasive candida infections, have low specificity and take up to 2-5 days to grow. we present the results of the first extensive multicenter clinical trial of a new nanodiagnostic approach, t2 magnetic resonance (t2mr), for diagnosis of candidemia. | 2015 | 25586686 |
| defining the transcriptomic landscape of candida glabrata by rna-seq. | candida glabrata is the second most common pathogenic candida species and has emerged as a leading cause of nosocomial fungal infections. its reduced susceptibility to antifungal drugs and its close relationship to saccharomyces cerevisiae make it an interesting research focus. although its genome sequence was published in 2004, little is known about its transcriptional dynamics. here, we provide a detailed rna-seq-based analysis of the transcriptomic landscape of c. glabrata in nutrient-rich me ... | 2015 | 25586221 |
| chemical composition and antimicrobial evaluation of the essential oils of bocageopsis pleiosperma maas. | essential oils from the leaves, twigs and barks of bocageopsis pleiosperma maas were obtained by using hydrodistillation and analysed by using gas chromatography coupled to mass spectrometry. several compounds (51) were detected and identified, being β-bisabolene the main component in all aerial parts of the plant, with higher concentration in the leaves (55.77%), followed by barks (38.53%) and twigs (34.37%). in order to increase the biological knowledge about the essential oil of bocageopsis s ... | 2015 | 25562370 |
| [yeast species in vulvovaginitis candidosa]. | vulvovaginal candidiasis is the most common mycosis, however, the available information about antifungal susceptibilities of these yeasts is limited. | 2015 | 25544052 |
| rate of fks mutations among consecutive candida isolates causing bloodstream infection. | precise fks mutation rates among candida species are undefined because studies have not systematically screened consecutive, disease-causing isolates. the sensititre yeastone (syo) assay measures echinocandin mics against candida with less variability than reference broth microdilution methods. however, clinical breakpoint mics may overstate caspofungin nonsusceptibility compared to other agents. our objectives were to determine candida fks mutation rates by studying consecutive bloodstream isol ... | 2015 | 26392494 |
| peptides from the scorpion vaejovis punctatus with broad antimicrobial activity. | the antimicrobial potential of two new non-disulfide bound peptides, named vpamp1.0 (lpffllslipsaisaikki, amidated) and vpamp2.0 (fwgflgklamkavpsliggnksssk) is here reported. these are 19- and 25-aminoacid-long peptides with +2 and +4 net charges, respectively. their sequences correspond to the predicted mature regions from longer precursors, putatively encoded by cdnas derived from the venom glands of the mexican scorpion vaejovis punctatus. both peptides were chemically synthesized and assayed ... | 2015 | 26352292 |
| draft genome of a commonly misdiagnosed multidrug resistant pathogen candida auris. | candida auris is a multidrug resistant, emerging agent of fungemia in humans. its actual global distribution remains obscure as the current commercial methods of clinical diagnosis misidentify it as c. haemulonii. here we report the first draft genome of c. auris to explore the genomic basis of virulence and unique differences that could be employed for differential diagnosis. | 2015 | 26346253 |
| evaluation of the "dip effect" phenomenon in antifungal susceptibility testing of candida spp. against echinocandins by use of gradient concentration strips. | the "dip effect" phenomenon complicates antifungal susceptibility testing with gradient concentration strips. of 60 candida isolates tested with the three echinocandins, this phenomenon was observed only for caspofungin with most (>90%) candida albicans, candida glabrata, and candida tropicalis isolates and for isolates with clsi mics of ≤0.25 mg/liter. in order to facilitate mic determination, a practical approach was developed using the inhibition zones at 32, 8, 2, and 1 mg/liter, increasing ... | 2015 | 26338855 |
| prevalence and fluconazole susceptibility profile of candida spp. clinical isolates in a brazilian tertiary hospital in minas gerais, brazil. | candidiasis has become an important concern for clinical practice, especially with the increasing incidence of immunocompromised patients. in this scenario, the development resistance to fluconazole presents a challenge for treating these opportunistic infections. the aim of this study was to evaluate some epidemiology features of candida infections in a brazilian university hospital using data, previously unavailable. we observed that 44% of the 93 clinical isolates tested, belonged to candida ... | 2015 | 26312420 |
| yeast microbiota of natural cavities of manatees (trichechus inunguis and trichechus manatus) in brazil and its relevance for animal health and management in captivity. | the aim of this study was to characterize the yeast microbiota of natural cavities of manatees kept in captivity in brazil. sterile swabs from the oral cavity, nostrils, genital opening, and rectum of 50 trichechus inunguis and 26 trichechus manatus were collected. the samples were plated on sabouraud agar with chloramphenicol and incubated at 25 °c for 5 days. the yeasts isolated were phenotypically identified by biochemical and micromorphological tests. overall, 141 strains were isolated, of w ... | 2015 | 26308797 |
| antifungal activity of 14-helical β-peptides against planktonic cells and biofilms of candida species. | candida albicans is the most prevalent cause of fungal infections and treatment is further complicated by the formation of drug resistant biofilms, often on the surfaces of implanted medical devices. in recent years, the incidence of fungal infections by other pathogenic candida species such as c. glabrata, c. parapsilosis and c. tropicalis has increased. amphiphilic, helical β-peptide structural mimetics of natural antimicrobial α-peptides have been shown to exhibit specific planktonic antifung ... | 2015 | 26287212 |
| characterization of cell wall proteins in saccharomyces cerevisiae clinical isolates elucidates hsp150p in virulence. | the budding yeast saccharomyces cerevisiae has recently been described as an emerging opportunistic fungal pathogen. fungal cell wall mannoproteins have been demonstrated to be involved in adhesion to inert surfaces and might be engaged in virulence. in this study, we observed four clinical isolates of s. cerevisiae with relatively hydrophobic cell surfaces. yeast cell wall subproteome was evaluated quantitatively by liquid chromatography/tandem mass spectrometry. we identified totally 25 cell w ... | 2015 | 26270963 |
| synergistic effects of tea catechin epigallocatechin gallate and antimycotics against oral candida species. | epigallocatechin gallate (egcg), the major antimicrobial tea polyphenol, has been reported to inhibit the growth of candida albicans planktonic cells and enhance the antifungal activity of antimycotics. we hypothesised that synergism exists between egcg and conventional antimycotics against biofilms of candida species. | 2015 | 26263544 |
| inpatient ophthalmology consultation for fungemia: prevalence of ocular involvement and necessity of funduscopic screening. | to determine the generalizability of recent data assessing the necessity of ophthalmic consultation for fungemic patients, we examined the prevalence, microbial profile, and treatment of fungal chorioretinitis and endophthalmitis among patients with positive fungal cultures referred for ophthalmologic consultation at a tertiary care medical center. | 2015 | 26235339 |
| sensitive and rapid rt-qpcr quantification of pathogenic candida species in human blood. | for accurate diagnosis and appropriate treatment of candidiasis, we developed a highly sensitive quantitative rt-pcr (rt-qpcr) system for five candida species that have been reported to be the major causes of bloodstream fungal infection (candida albicans, candida glabrata, candida tropicalis, candida parapsilosis, and candida krusei), together with a system for all pathogenic candida species. cells of each fungal species spiked into human peripheral blood (pb) were specifically detected at a lo ... | 2015 | 26232708 |
| synthesis and antifungal evaluation of head-to-head and head-to-tail bisamidine compounds. | herein, we describe the antifungal evaluation of 43 bisamidine compounds, of which 26 are new, having the scaffold [am]-[hetar]-[linker]-[hetar]-[am], in which [am] is a cyclic or acyclic amidine group, [linker] is a benzene, pyridine, pyrimidine, pyrazine ring, or an aliphatic chain of two to four carbon, and [hetar] is a 5,6-bicyclic heterocycle such as indole, benzimidazole, imidazopyridine, benzofuran, or benzothiophene. in the head-to-head series the two [hetar] units are oriented such that ... | 2015 | 26209266 |
| delicate metabolic control and coordinated stress response critically determine antifungal tolerance of candida albicans biofilm persisters. | candida infection has emerged as a critical health care burden worldwide, owing to the formation of robust biofilms against common antifungals. recent evidence shows that multidrug-tolerant persisters critically account for biofilm recalcitrance, but their underlying biological mechanisms are poorly understood. here, we first investigated the phenotypic characteristics of candida biofilm persisters under consecutive harsh treatments of amphotericin b. the prolonged treatments effectively killed ... | 2015 | 26195524 |
| retrospective analysis of mortality and candida isolates of 75 patients with candidemia: a single hospital experience. | the mortality rate for candidemia is approximately 30%-60%. however, prognostic factors in patients with candidemia have not yet been elucidated in detail. the aim of the present study was to analyze prognostic factors for candidemia using the mortality rate and candida isolates of patients with candidemia. seventy-five patients with candidemia were analyzed between january 2007 and december 2013. the main outcome of this study was the 30-day mortality rate after the diagnosis of candidemia. the ... | 2015 | 26185460 |