Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| metronidazole bioassay with increased sensitivity. | the bioassay of metronidazole using clostridium butyricum incorporated in agar plates detected concentrations higher than 1.0 microgram/ml. gentamicin in the sample did not affect the growth of the target organism or the inhibition by metronidazole. penicillin in the sample could be eliminated by the incorporation of penicillinase in the agar or by using as the target organism a surface inoculum of the penicillin-resistant bacteroides fragilis. increasing duration and temperature of aerobic pred ... | 1979 | 220515 |
| [development of an accelerated method of determining the antibiotic sensitivity of cl. perfringens type a]. | an express method for determination of antibiotic sensitivity in the strains of cl. perfringens of type a using soviet dry nutrient media and antibiotics is proposed. the criteria for estimation of the level of the antibiotic sensitivity of the causative agent of gas gangrene in short periods on the basis of comparison of the data of the antibiotic agar diffusion procedure and the antibiotic mic were worked out. twelve antibiotics and 45 collection strains of cl. perfringens of type a were used ... | 1979 | 219770 |
| fluid accumulation in mouse ligated intestine inoculated with clostridium perfringens enterotoxin. | clostridium perfringens enterotoxin, when inoculated into the ligated intestinal loop of mice, caused marked distension due to fluid accumulation. the increase in weight of the intestinal loop was proportional to the log dose of enterotoxin within a range from 1 to 16 micrograms. the fluid accumulation was arrested by washing the loop with saline or by injection of the specific anti-enterotoxin serum into the loop 5 or even 30 min after inoculation of the enterotoxin. a significant increase in w ... | 1979 | 219772 |
| anaerobic infections. | 1979 | 219788 | |
| in vitro activity of cefoxitin and parenterally administered cephalosporins against anaerobic bacteria. | the in vitro activity of cefoxitin against 221 recent isolates of anaerobic bacteria was compared with that of cefamandole and cefuroxime by the agar dilution technique. at achievable serum levels of 32 micrograms/ml, all three drugs were active against most groups of anaerobic bacteria tested. cefoxitin was active against most strains of the bacteroides fragilis group, whereas cefamandole and cefuroxime were relatively ineffective. cefoxitin was least active against clostridium species other th ... | 1979 | 318226 |
| the effects of temperature and ethidium bromide on the banding of heat-denatured dna in gradients of nai. | the effects of temperature and ethidium bromide on the banding of heat-denatured dna was studied during equilibrium centrifugation in density gradients of nai. centrifugation at 10 degrees c prevents the partial renaturation of escherichia coli dna and clostridium perfringens dna that occurs at 20 degrees c. a centrifugation temperature of --5 degrees c is required to prevent renaturation of t7 phage dna. ethidium bromide decreases renaturation of escherichia coli dna during centrifugation at 20 ... | 1979 | 369610 |
| acute appendicitis and bacteroides fragilis. | bacteria belonging to the bacteroides fragilis group (b. fragilis, b. ovatus, b. vulgatus, b. distasonis, b. thetaiotaomicron and b. uniformis) were cultured in quantities of less than or equal to 10(5) c.f.u./ml from the excised appendix from 30 patients. twenty-two patients (group i) had an acute purulent appendicitis (three with perforation), four were classified as slightly inflamed cases (group 2) and four had a normal appendix (group 3). the b. fragilis group of bacteria dominated among th ... | 1979 | 379991 |
| controlled cell disruption: a comparison of the forces required to disrupt different micro-organisms. | a cell disrupter has been developed which can measure the forces required to disrupt both eukaryotic and prokaryotic cells. it operates a continuous process and will disrupt both large and small volumes. shear forces are set up when a suspension under laminar flow conditions is released under high pressure through a short orifice. if the applied pressure is altered, the shear forces are simultaneously changed so that the amount of cell disruption can be compared under different known and repeata ... | 1979 | 422681 |
| [intestinal gas gangrene originating from the gallbladder: diagnosis, therapy, report of 2 cases]. | the problems of diagnosis and therapy of gas gangrene of the gastrointestinal tube and the gallbladder are discussed. 2 cases of gas edema of the gallbladder due to clostridium perfringens are reported. one of the patients survived. the early diagnosis of gas gangrene is important because of the extraordinary way of infection and the necessary early surgical and medicamentous therapy. | 1979 | 447171 |
| core nucleosomes by digestion of reconstructed histone-dna complexes. | reconstructed complexes of the inner histones (h2a, h2b, h3, h4) and a variety of dnas were digested with micrococcal nuclease to yield very homogeneous populations of core nucleosomes (nu 1). nucleosomes containing micrococcus luteus dna (72% g+c); chicken dna (43% g+c), clostridium perfringens dna (29% g+c); or poly(a-dt.poly(da-dt) have been examined by circular dichroism, thermaldetenaturation, electron microscopy, and dnase i digestion. circular dichroism spectra of all particles show a ty ... | 1979 | 450703 |
| emphysematous cystitis associated with clostridium perfringens bacteremia. | anaerobes are recognized rarely as the cause of urinary tract infection. a case is reported in which there were clinical signs of sepsis, positive blood culture for clostridium perfringens and radiographic demonstration of emphysematous cystitis without any other recognized source of infection. | 1979 | 458961 |
| monomer-micelle transition of the ganglioside gm1 and the hydrolysis by clostridium perfringens neuraminidase. | the action of clostridium perfringens neuraminidase on the ganglioside gm1 tritiated in the ceramide moiety was studied. the rates of hydrolysis of the gm1 ganglioside were determined from radioactivity in the neutral glycolipid product, which was separated from the substrate on deae-sephadex columns. in order to study the physical state of the substrate in the conditions used in the neuraminidase treatment, the critical micelle concentrations of the gm1 ganglioside were determined using formati ... | 1979 | 467431 |
| action of arthrobacter ureafaciens sialidase on sialoglycolipid substrates. mode of action and highly specific recognition of the oligosaccharide moiety of ganglioside gm1. | a new bacterial sialidase (n-acetylneuraminate glycohydrolase, ec 3.2.1.18) isolated from the culture filtrate of arthrobacter ureafaciens was characterized in detail with respect to its action on sialoglycolipids. strong electrolytes had a reversible inhibitory effect on the action of the enzyme on brain gangliosides in accordance with debye-hückel effect of ionic environment on ionic activity, and resulted in an acidic shift and a broadening of the ph optimum. both ionic and non-ionic detergen ... | 1979 | 468792 |
| survival of anaerobic bacteria in common laboratory diluents. | the survival of six species of anaerobic bacteria was studied in simple or commercially available diluents. bacteroides fragilis and fusobacterium nucleatum showed excellent survival in all diluents including distilled water. fusobacterium mortiferum survived well in all diluents except water and water supplemented with 0.1% gelatain. clostridium perfringens survived best in phosphate-buffered saline with gelatin. peptococcus asaccharolyticus required gelatin added to the basic diluent, and stre ... | 1979 | 479359 |
| deoxyribonucleic acid binding studies on several new anthracycline antitumor antibiotics. sequence preference and structure--activity relationships of marcellomycin and its analogues as compared to adriamycin. | the deoxyribonucleic acid (dna) binding characteristics of adriamycin and several new anthracycline glycosides, including marcellomycin, aclacinomycin, rudolfomycin, musettamycin, and pyrromycin, have been studied. the fluorescence spectra were determined for all six anthracyclines, and the fluorescence quenching effects caused by interactions with the natural dnas poly(dadt)--poly(dadt) and poly(dgdc) were characterized. binding parameters were determined by scatchard analyses of results obtain ... | 1979 | 486409 |
| food poisoning in hospitals in scotland. | a review of 50 hospital-based outbreaks of food poisoning which were reported in scotland during 1973--7, is described. at least 1530 persons consuming hospital-prepared food were involved. thirty-one episodes were associated with clostridium perfringens (c. welchii), 11 were due to food-borne salmonella infection, three to enterotoxigenic staphylococcus aureus, and five incidents were of undetermined aetiology. this differs noticeably from the experience in england and wales where salmonellas a ... | 1979 | 489961 |
| clostridium perfringens associated with a focal abscess in a horse. | 1979 | 500433 | |
| collaborative evaluation of a proposed reference dilution method of susceptibility testing of anaerobic bacteria. | an agar dilution method for susceptibility testing of anaerobic bacteria was evaluated to determine whether results obtained would be consistent enough to recommend it as a reference method. the study was conducted in 10 laboratories where the minimum inhibitory concentrations of six antibiotics (carbenicillin, cefoxitin, chloramphenicol, clindamycin, penicillin g, and tetracycline) were determined against 10 bacterial strains on wilkins-chalgren agar prepared by three manufacturers. minimum inh ... | 1979 | 518079 |
| in vitro activity of ly127935. | ly127935 is a unique new beta-lactam antibiotic. its activity against 536 clinical isolates was studied by using microdilution methods of susceptibility testing and compared with the activities of cefamandole, cefoxitin, and cephalothin. the lowest concentrations required to inhibit at least 90% of strains tested (mic(90)s) of ly127935 for staphylococcus aureus, streptococcus pyogenes, streptococcus agalactiae, and streptococcus pneumoniae ranged from 2 to 8 mug/ml. the mic(90)s for other staphy ... | 1979 | 518080 |
| asymmetric manipulation of the membrane lipid bilayer of intact human erythrocytes with phospholipase a, c, or d induces a change in cell shape. | changes in the membrane morphology and phospholipid content of human erythrocytes were determined after incubation of intact cells with each of various exogeneous phospholipases (plases). plase a2 from naja naja or bee venom induced crenation of the cells in parallel with hydrolysis of the membrane phosphatidylcholine (pc). this crenated cell shape was reversed to a biconcave disc or cup-like form by a further treatment with lysophospholipase. in contrast, bacterial plase c from clostridium perf ... | 1979 | 521437 |
| gas gangrene and clostridium perfringens septicemia associated with the use of an indwelling radial artery catheter. | 1979 | 534977 | |
| role of clostridial toxin in the pathogenesis of clindamycin colitis in rabbits. | the pathophysiology of antibiotic-associated colitis was studied in rabbits with severe ileocolitis induced by oral administration of clindamycin. cell-free, sterile filtrates of cecal contents of rabbits with clindamycin colitis contained a toxin that was lethal for mice and cytotoxic for hela-cell monolayers. the toxin was heat labile, was inactivated by pronase but not trypsin, and had a mol wt by gel filtration on sephadex g-100 of 45,000. the toxin was neutralized by antiserum to clostridiu ... | 1979 | 759264 |
| the carbon dioxide requirements of anaerobic bacteria. | some clinically significant anaerobic bacteria were incubated in pure culture in anaerobic jars containing a range of atmospheric concentrations of co2. growth of clostridium perfringens, c. sporogenes and c. septicum was independent of the amount of co2. small supplements of co2 (0.25%) allowed good growth of the majority of anaerobes studied--an observation contrary to established teaching. the exceptions were fusobacterium necrophorum which showed an absolute requirement for co2 of at least 1 ... | 1980 | 6107383 |
| decrease in virulence of some well characterized pathogenic bacterial strains when grown in presence of infection. | 1980 | 6163699 | |
| interactions of bleomycin analogues with deoxyribonucleic acid and metal ions studied by fluorescence quenching. | 1980 | 6155142 | |
| [dna--helix destabilizing proteins (author's transl)]. | 1980 | 6156442 | |
| characterization of membrane permeability alterations induced in vero cells by clostridium perfringens enterotoxin. | alterations in plasma membrane permeability induced by clostridium perfringens enterotoxin were studied using vero (african green monkey kidney) cells which were radioactively labeled with four markers of different molecular size. the markers were alpha-amino[14c]isobutyric acid (mr 103), 3h-labeled nucleotide (mr approx. 300), 51cr label (mr approx. 3000) and [3h]rna (mr>25000). over a 2h period, enterotoxin caused significant release of aminoisobutyric acid, nucleotides and 51cr label but not ... | 1980 | 6157413 |
| [use of redox-fibers for culturing clostrium perfringens]. | 1980 | 6157930 | |
| comparison of media and methods for counting clostridium perfringens in poultry meat and further-processed products. | a most probable number (mpn) method involving differential reinforced clostridial medium followed by streaking on willis & hobbs medium was compared with direct plating of samples on tryptose-sulphite-cycloserine agar without egg yolk, and two forms of oleandomycin-polymyxin-sulphadiazine-perfringens agar, one being prepared from a commercial, dehydrated product. with skin samples taken from chicken carcasses at different stages of processing, the three direct plating media gave similar counts o ... | 1980 | 6243326 |
| mechanism of action of clostridium perfringens enterotoxin. effects on membrane permeability and amino acid transport in primary cultures of adult rat hepatocytes. | purified enterotoxin from the bacterium clostridium perfringens rapidly decreased the hormonally induced uptake of alpha-aminoisobutyric acid in primary cultures of adult rat hepatocytes. at 5 min after toxin addition the decrease in alpha-aminoisobutyric acid uptake appeared not due to increased passive permeation (estimated with l-glucose) or to increased alpha-aminoisobutyric acid efflux. when short uptake assay times were employed a depression of alpha-aminoisobutyric acid influx was observe ... | 1980 | 6243482 |
| indicator bacteria and salmonella in food-processing and domestic effluent. | 1980 | 6243713 | |
| acute emphysematous cholecystitis: an ultrasonic diagnosis. | 1980 | 6243848 | |
| the synthesis of 4-methylumbelliferyl alpha-ketoside of n-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase. | 1980 | 6243885 | |
| enumeration of potentially pathogenic bacteria from sewage sludges. | to ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. a processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers of viable bacteria. conventional methods were successful in enumerating klebsiella, staphylococcus, gram-negative enteric bacteria, and commonly used indicator organisms. modificatio ... | 1980 | 6243900 |
| hydrolysis of di- and trisialo gangliosides in micellar and liposomal dispersion by bacterial neuraminidases. | the hydrolysis of di- and trisialo gangliosides by bacterial neuraminidases was investigated. slow rates of hydrolysis were obtained with micellar dispersions of the pure gangliosides; the rates increased considerably with mixtures of ganglioside and phospholipids, such as phosphatidylcholine or sphingomyelin. the greatest rates of hydrolysis were obtained with mixtures containing 5-10 mol% ganglioside and 90-95% phospholipid. with the aid of the nonpenetrating reagent trinitrobenzenesulfonic ac ... | 1980 | 6243952 |
| studies on a mammalian hepatic binding protein specific for asialoglycoproteins. evidence for receptor recycling in isolated rat hepatocytes. | freshly isolated rat hepatocytes attained maximal ability to bind, internalize, and degrade 125i-asialo-orosomucoid after 5 h in suspension culture at 37 degrees c. comparison of the number and distribution of the asialoglycoprotein binding sites of these cells revealed that 5% (6.7 x 10(4) receptors/cell) were on the external cell membrane with an average residency time of slightly less than 3 min. the remaining 95% were located intracellularly, as determined with detergent-solubilized hepatocy ... | 1980 | 6244303 |
| studies of enzyme-mediated reactions. part 13. stereochemical course of the formation of histamine by decarboxylation of (2s)-histidine with enzymes from clostridium welchii and lactobacillus 30a. | 1980 | 6244319 | |
| commercial milk products and indigenous weaning foods in a rural west african environment: a bacteriological perspective. | two commercially available baby milks, one 'biologically acidified', the other 'non-acidified', and a traditional weaning food, millet gruel, were prepared and stored under village conditions in west africa. increases in total colony count and in number of bacillus cereus, clostridium welchii, staphylococcus aureus and escherichia coli were determined in these products when stored as commonly practised at ambient temperatures over a period of 8 h. poor hygiene during preparation was indicated by ... | 1980 | 6244343 |
| hospitals should do the sick no harm. 2. why are bacteria harmful? | 1980 | 6244533 | |
| hydrolysis of gangliosides in micellar and liposomal dispersion by bacterial neuraminidases. | aqueous dispersions of pure gangliosides contain micelles of these compounds. in this dispersion state, the rates of hydrolysis of the neuraminyl residues by bacterial neuraminidases are slowest. incorporation of gangliosides into mixed dispersion with other lipids or into mixed micelles with bile salts considerably increases the reaction rates. the greatest reaction rates are obtained when di- or trisialogangliosides are incorporated into unilamellar vesicles of lecithin or sphingomyelin. | 1980 | 6244720 |
| clostridium perfringens as a water pollution indicator. | 1980 | 6245283 | |
| [gas gangrene caused by bacteria, other than clostridium, and diabetes mellitus. a study of two cases (author's transl)]. | 1980 | 6245313 | |
| [the incubation period of gas gangrene (author's transl)]. | 1980 | 6245494 | |
| [early diagnosis and treatment of anaerobic infection]. | 1980 | 6245535 | |
| phosphatidylglycerophosphate synthease and phosphatidylserine synthase activites in clostridium perfringens. | cytidine 5'-diphospho (cdp)-1,2-diacyl-sn-glycerol (cdpdiacylglycerol):sn-glycerol-3-phosphate phosphatidyltransferase (ec 2.7.8.5, phosphatidylglycero-p synthase) and cdpdiacylglycerol:l-serine o-phosphatidyltransferase (ec 2.7.8.8, phosphatidylserine synthase) activities were identified in the cell envelope fraction of the gram-positive anaerobe clostridium perfringens. the association of phosphatidylglycero-p synthase and phosphatidylserine synthase with the cell envelope fraction of cell-fre ... | 1980 | 6246064 |
| purification to homogeneity of h blood group beta-galactoside alpha 1 leads to 2 fucosyltransferase from porcine submaxillary gland. | a beta-galactoside alpha 1 leads to 2 fucosyltransferase has been solubilized from porcine submaxillary glands and purified 124,000-fold to homogeneity by repeated affinity chromatography on gdp-hexanolamine agarose. sodium dodecyl sulfate polyacrylamide gel electrophoresis of the purified enzyme revealed two electrophoretic species with apparent mr = 60,000 and 55,000. the two enzyme species have not been completely resolved, but both appear to be active forms of the fucosyltransferase with app ... | 1980 | 6246105 |
| binding of enterotoxin from clostridium perfringens type a to liver cells in vivo and in vitro. the enterotoxin causes membrane leakage. | enterotoxin from clostridium perfringens was shown to retain its biological activity after labelling with 125i. when injected intravenously into mice and rats, most of the radioactivity in the organs was present in the form of intact toxin. studies of the tissue distribution of labelled enterotoxin showed the largest amounts in the liver, where the activity reached a maximum 10--15 min after administration. the highest concentration per g tissue was found in liver and kidneys. the radioactivity ... | 1980 | 6246712 |
| characterization of a new type of glycoprotein saccharides containing polysialosyl sequence. | 1980 | 6246895 | |
| rapid procedures for determination of endo-n-acetyl-alpha-d-galactosaminidase in clostridium perfringens, and of the substrate specificity of exo-beta-d-galactosidases. | culture fluid of clostridium perfringens hydrolyzed the synthetic, chromogenic substrates beta-gal-(1 leads to 3)-alpha-galnac-1 leads to oph and beta-gal-(1 leads to 3)-alpha-galnac-1 leads to oc6h4-no2-o or -p to beta-gal-(1 leads to 3)-galnac and the aglycon. such assays facilitated the characterization and purification of this endo-n-acetyl-alpha-d-galactosaminidase activity. this activity was purified 1200-fold by fractionation with ammonium sulfate and chromatography on columns of sephadex ... | 1980 | 6247065 |
| foodborne disease outbreaks in connecticut during 1977 and 1978. | 1980 | 6247119 | |
| [properties of e. coli, salm. typhimurium and cl. perfringens strains isolated from soil containing bacterial insecticides based on bac. thuringiensis]. | 1980 | 6247250 | |
| [epidemiology of nosocomial clostridial myonecroses (gas gangrene)]. | 1980 | 6247769 | |
| the effect of cadmium on indicator bacteria in sewage. | 1980 | 6247900 | |
| [transferable tetracycline resistance in "clostridium difficile" (author's transl)]. | tetracycline (tc) resistance is transferable from a resistant strain of clostridium difficile to a sensitive strain and this resistance is not curable. resistances to erythromycin and clindamycin are curable but not transferable. these results suggest for these resistances a plasmid determinism. it is shown that a plasmid-mediated tc resistance (pip401) of c. perfringens is also transferable to c. difficile. tc resistance is inducible in c. perfringens and constitutively expressed in c. difficil ... | 1980 | 6247949 |
| sensitive assay for measuring tetracycline levels in gingival crevice fluid. | an increased interest in the clinical use of antibiotics as an adjunct to periodontal therapy has created a need to determine antibiotic concentrations in fluid obtained from the gingival crevice. for this purpose, an increase in sensitivity beyond that possible with current tetracycline assays is essential because sample volumes of gingival fluid typically obtained are less than 0.5 microliter. this report describes the development of an agar-diffusion assay technique capable of measuring the c ... | 1980 | 6247968 |
| reversal of radiation-dependent heat sensitization of clostridium perfringens spores. | the effect of solute concentration on the sensitization of clostridium perfringens spores to heat by ionizing radiation was investigated. as we have shown previously, spores of c. perfringens treated with gamma radiation are now sensitive to subsequent heat treatments than are spores that receive no radiation treatment. when gamma-irradiated spores were heated in the presence of increasing concentrations of glycerol or sucrose, the heat sensitivity induced by irradiation was progressively decrea ... | 1980 | 6247972 |
| relationships between environmental factors, bacterial indicators, and the occurrence of enteric viruses in estuarine sediments. | current standards for evaluation of the public health safety of recreational and shellfish-harvesting waters are based upon bacteriological analysis, but do not include an evaluation of the number of viruses. the objective of this study was to determine the occurrence of enteric viruses in estuarine sediments and to find a relationship, if any, between the presence of viruses in seawater or sediment or both and various biological and physicochemical characteristics of the environment. viruses we ... | 1980 | 6247974 |
| [changes in the serum acid phosphatase activity of guinea pigs poisoned with c1 perfringens type a toxin and a mixture of toxin and a broth culture filtrate]. | it was shown in experiments on guinea pigs that the injection of c1. perfringens type a toxoid induced an increase in acid phosphatase activity of animal blood serum. the action of the toxoid increased under the effect of c1. butyricum cultural filtrate, which gave rise to an earlier enhancement of the specific activity of the enzyme as compared to the injection of the toxoid alone. increased activity of acid phosphatase may play a pathogenetic role in cases of anaerobic infection caused by asso ... | 1980 | 6248148 |
| empyema caused by clostridium perfrigens. | 1980 | 6248609 | |
| fatal clostridium perfringens sepsis; following malignant hemangioendothelioma of scalp. | 1980 | 6248825 | |
| [ecg tracing in electric injuries and subsequent burns complicated by chest contusion and anaerobic septicemia]. | 1980 | 6248996 | |
| the ganglioside content of the milk fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected mice. partial characterization of a new disialoganglioside. | the milk fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected swiss mice have been investigated for their content in gangliosides. when compared on the lipid phosphorus basis, viral envelope is found to contain more than twice as much lipid-bound sialic acid as fat-globule membrane. the ganglioside patterns of these two structures appear rather similar, except for the occurrence in fat-globule membrane of a low ganglioside homolog, presumably gm2, not detect ... | 1980 | 6249587 |
| synergistic haemolysis test for presumptive identification and differentiation of clostridium perfringens, c. bifermentans, c. sordellii, and c. paraperfringens. | a new test for the presumptive identification of clostridium perfringens, c. bifermentans, c. sordellii, and c. paraperfringens is described. the test is based on the synergistic haemolysis shown by the clostridia and group b streptococci on sheep and human and cacl2-supplemented human blood agar. c. perfringens gave crescent-shaped synergistic lytic zones (7 to over 20 mm in length), and c. paraperfringens usually small-sized (3 mm), bullet-shaped reactions on all three types of media. c. bifer ... | 1980 | 6249855 |
| comparison of methods available for assay of chloramphenicol in clinical specimens. | eight methods for the assay of chloramphenicol in clinical samples were compared with our own modification of a plate diffusion technique using sarcina lutea and yeast extract agar. six of the eight methods were less sensitive than originally reported, and five of them were considered unsuitable for use in clinical microbiology practice. the remaining three methods together with the s. lutea/yeast extract modification were used to assay chloramphenicol in 20 samples of serum. twenty samples of c ... | 1980 | 6249858 |
| [clostridium sepsis as a result of acute gangrenous cholecystitis]. | 1980 | 6250228 | |
| improved method for purification of enterotoxin from clostridium perfringens type a. | the purification procedure of clostridium perfringens type a enterotoxin has been improved. the cell sonic extract was precipitated twice with ammonium sulfate, first 40% saturated to concentrate the enterotoxin and then 15% saturated. the two precipitations were followed by gel filtration on sephadex g-100. the enterotoxin appeared to be homogeneous on 7% polyacrylamide gel electrophoresis after this three-step purification procedure, with a recovery of 56% and a 12.3-fold purification. the sol ... | 1980 | 6250473 |
| [clostridium perfringens infection and necrotising enterocolitis]. | during a four year period (1974--1977) 21 infants died as a result of severe necrotising enterocolitis (n.e.c.). in 9 cases, clostridium perfringens was isolated. when this organism is recovered either from the placenta or from the first meconium and or when the signs of the disease appear within a few days of birth, materno-fetal transmission of the infection may be suspected. the infection occurs most frequently in neonates in whom the gastrointestinal tract was already colonized by clostridiu ... | 1980 | 6250508 |
| the influence of glycosidases and lectins on insulin binding to zajdela hepatoma cells. | 1980 | 6250906 | |
| scanning electronmicroscopy of the jejunum in enteritis necroticans. | intestinal tissue resected at laparotomy from patients in papua new guinea at various clinical stages of enteritis necroticans, locally known as pig-bel, has been examined under the scanning electronmicroscope. evidence obtained from parallel studies of experimental infection in pigs is presented. progressive destruction of the intestinal mucosa was seen during the course of the disease in man. numerous filamentous rods morphologically consistent with the appearance of clostridium perfringens ty ... | 1980 | 6251221 |
| clostridium perfringens wound infection associated with elastic bandages. | clostridium perfringens wound infections were associated with the use of nonsterile elastic outer bandages in diabetic patients who had undergone lower extremity amputation for vascular insufficiency. in each case a second surgical procedure was required. elastic bandages similar to those used in these procedures were found to contain c perfringens and other clostridial species. this report illustrates the need for maintenance of a sterile, nonpermeable inner barrier to prevent transudation of b ... | 1980 | 6251286 |
| effect of oxidizing agents and sulfhydryl group reagents on beta toxin from clostridium perfringens type c. | purified beta toxin from clostridium perfringens type c was inactivated by the oxidizing agents o-iodosobenzoate (oiba), oxidized glutathione, and ferricyanide, and by the sulfhydryl group regents 5,5'-dithio-bis(2-nitrobenzoic acid) (dtnb) and n-ethylmaleimide, iodoacetamide, and iodoacetic acid, causing loss of activity in various degrees depending on the concentration used. the activity of the toxin was not influenced by exposure to 1.0 mm of p-chloromercuribenzoate. the toxin treated by oiba ... | 1980 | 6251344 |
| the control of bacterial contamination in carcass meal with propionic acid. | the effect of the addition of 2, 3, 5, 7 and 10% respectively of propionic acid on known numbers of bacteria added to or contained in carcass meal was examined. escherichia coli was totally inhibited by 2% propionic acid, while 5% of the acid both inhibited salmonella typhimurium and brought about a 74,7% reduction in the total aerobic bacterial count. | 1980 | 6251412 |
| microbiology of the canine nasal cavities. | the anterior and superior regions of canine nares yielded two distinct microbial populations consisting of 25 microbial species. streptococci predominated in the anterior nose; clostridia and staphylococci were also quite prevalent. at least two distinct microorgranisms were cultured from most anterior sites. in contrast, sterile conditions prevailed in the superior nasal regions of at least 50% of the ninety-two animals. various bacillus sp. were recovered from the remainder. | 1980 | 6251533 |
| clostridium perfringens food-borne disease and bacteriological analysis for strain identification. | 1980 | 6251778 | |
| clostridial pleuropulmonary infection. | primary clostridial pleuropulmonary infection occurred in two patients. the pathogenesis of infection was related to aspiration of oropharyngeal contents or hematogenous seeding of infarcted lung tissue. one patient was cured with penicillin; the other died secondary to sepsis and respiratory failure. review of 13 additional cases from the literature demonstrated pulmonary infection due to clostridia to be characterized by lobar or multilobar disease with uniform involvement of the pleura. iatro ... | 1980 | 6251997 |
| clostridium perfringens type a: in vitro system for sporulation and enterotoxin synthesis. | polysomes were isolated from an enterotoxigenic strain of clostridium perfringens during vegetative growth and at 1-h intervals after transfer into duncan-strong sporulation medium. during vegetative growth, about 67% of the ribosomes were in polysomal complexes. this proportion decreased to about 20% during the first 2 h in sporulation medium and then gradually increased to a maximum of 45% at 6 h. ribosomes isolated from cells in vegetative or in sporulation phase could equally translate veget ... | 1980 | 6252194 |
| evaluation of a clostridial alpha-toxin disk test for rapid presumptive identification of group b streptococci. | an alpha-toxin disk test is described in which group b streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of clostridium perfringens. the test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. a total of 95% of strains of pure group b streptococci tested produced positive reactions within 5 h, and all were positive after overnight incubation, with patterns of synergistic hemolysis readily distingu ... | 1980 | 6252240 |
| new presumptive identification test for clostridium perfringens: reverse camp test. | the reverse camp test proved to be a highly sensitive test since 97.0% of all clostridium perfringens cultures tested gave an easily discernible positive reaction. | 1980 | 6252247 |
| incidence of increased numbers of clostridium perfringens in the intestinal tract of rats fed xylitol. | sprague-dawley rats were fed diets containing up to 20% xylitol for 49 days. when the rats were fed a xylitol regimen intended to produce adaptation to xylitol, approximately half of the animals adapted to xylitol and remained free from diarrhea during the feeding regimen. the other half did not adapt to xylitol and developed severe and persistent diarrhea accompanied by large volumes of intestinal gas. these non-adapted rats had significantly higher levels of intestinal tract clostridium perfri ... | 1980 | 6252305 |
| ampicillin-induced enterocolitis: implication of toxigenic clostridium perfringens type c. | 1980 | 6252310 | |
| a cluster of septic complications associated with illegal induced abortions. | between august 7 and october 14, 1977, clostridium perfringens organisms were isolated from endometrial and/or blood specimens from 3 women who had been hospitalized in mcallen, texas, after having had illegal induced abortions. one of the women died of clostridial septicemia. a single abortionist was suspected in these 3 cases. the authors investigated the illnesses of these 3 women and those of 6 other women who were hospitalized at some time from january through october 1977 after having had ... | 1980 | 6252522 |
| the effects of clostridium perfringens type a enterotoxin in shetland ponies--clinical, morphologic and clinicopathologic changes. | severe abdominal pain, classic colic signs and hemorrhagic gastro-entero-cecocolitis were induced in three conventional shetland ponies by intravenous injection with clostridium perfringens type a enterotoxin. histological examination showed marked congestion, edema and hemorrhage of the large and small intestine and sloughing of the tips of the intestinal villi. marked vacuolar degeneration of hepatocytes with dilatation of the spaces of disse also was found. clinical changes consisted of sever ... | 1980 | 6252678 |
| [problems of maintaining hygienic and microbiological quality standards in the production of foodstuffs, particularly those of vegetable origin. second report: demands regarding hygiene and microbiology-theory is useless without practice (author's transl)]. | many legal regulations exist on the hygienic-microbiological properties of food products. the 'länder-speiseeisverordnungen' (county ice cream regulations), the 'diät-verordnung' (dietary regulation) and the 'trinkwasser-verordnung' (regulation on potable water) are examples for the frequent inadequacies inherent in these regulations. caution is indicated against any new proposals for the establishment of additional hygienic-microbiological regulations without sufficient practice-related reasoni ... | 1980 | 6252723 |
| [rendering of animal material in the netherlands (author's transl)]. | just before the world war ii rendering of all material of animal origin unfit for human and animal consumption was a fact and was later on regulated by decree in 1942. the rendering act dates from 1957 referring to as "processing into useful product". there is a compulsery rendering which means that withdrawal of the material mentioned from rendering is in general prohibited. material for rendering must be notified. in use is the dry rendering system (atmospheric batch cookers). at the moment th ... | 1980 | 6252724 |
| [computation for the sterilisation process in rendering plants (author's transl)]. | in the present paper a mathematical model for the computation and costing of the sterilisation process in rendering plants has been developed and the computation of the f value in the canning industry has been used as a guide. in this, a test germ with the heat resistance parameters z = 10 degrees c and dr = 10 sec is taken as a guide. if the steam-pressure heating regulation of the german carcass disposal act which prescribes a temperature of 133 degrees c during 20 min after decomposition of t ... | 1980 | 6252725 |
| experimental pigbel: the production and pathology of necrotizing enteritis due to clostridium welchii type c in the guinea-pig. | an animal model for pigbel in man was developed using guinea-pigs. intragastric dosing with growing cultures of clostridium welchii type c only produced necrotic lesions if protease inhibitors were given as well. beta toxin, which is made by the type c organism, causes the intestinal damage and is very easily destroyed by proteases. protease inhibitors in soybean and aprotinin were effectively in inducing disease in animals on a normal diet, while inhibitors in sweet potato, which inhibit only t ... | 1980 | 6252934 |
| binding of clostridium perfringens [125i]enterotoxin to rabbit intestinal cells. | 125i-labeled enterotoxin from clostridium perfringens was utilized to characterize the association of the enterotoxin with cells isolated from rabbit intestine and tissue homogenates from liver, kidney, and brain. the enterotoxin was found to bind in a specific and saturable manner to cells from intestine and to tissue homogenates from liver and kidney but not the brain. detailed studies of the binding were carried out with the ileal epithelial intestinal cells. the rate and amount of binding of ... | 1980 | 6252960 |
| clostridium perfringens in animal disease: a review of current knowledge. | the diseases caused by various types of clostridium perfringens are critically reviewed in the light of current knowledge. particular emphasis is placed on information concerning these diseases in canadian livestock. there are two etiologically clearly-defined acute c. perfringens diseases recognized in canada: hemorrhagic enteritis of the new born calf, caused by c. perfringens type c, and enterotoxemia of sheep, caused by type d. clostridium perfringens type a may play a role as a secondary pa ... | 1980 | 6253040 |
| pathogenesis of necrotising enteritis with special reference to intestinal hypersensitivity reactions. | the aetiological aspects of 83 sporadic cases of necrotising enteritis (ne) have been studied. of 56 cases in which histology of the intestine was possible, eight showed appearances (oedema and local eosinophilia) suggestive of a type i hypersensitivity reaction, while in 37 the appearances were suggestive of a type iii reaction. we suggest that these reactions, which were more common in children and young adults, were initiating factors in the intestinal necrosis. the type iii reactions (submuc ... | 1980 | 6253362 |
| [1h-nmr-spectroscopic evidence for the release of n-acetyl-alpha-d-neuraminic acid as the first product of neuraminidase action (author's transl)]. | the 1h-nmr spectroscopy was used to study the anomeric configuration of n-acetyl-d-neuraminic acid released by the action of neuraminidase. the hydrolysis of neuacalpha 2 leads to 3 gal-beta 1 leads to 4glc (20mm) by the enzymes of clostridium perfringens and arthrobacter ureafaciens (50 mu, 150 mu and 800 mu, respectively) in 50mm na/k-phosphate buffer pd 5.4 was observed by recording the spectra. on the basis of the characteristic signals of the protons at c-3 (alphaneuac: delta[h(3e)] = 2.72, ... | 1980 | 6253376 |
| interaction of streptolysin o from streptococcus pyogenes and theta-toxin from clostridium perfringens with human fibroblasts. | the membrane-damaging properties on human diploid embryonic lung fibroblasts of streptolysin o (from streptococcus pyogenes) and theta-toxin (from clostridium perfringens) were compared. the results are consistent with the suggested mechanism for hemolysis by streptolysin o involving one fixation site and one lytic site of this cytolysin. however, the membrane-damaging activity of the two toxins differed with respect to (i) relative cytolytic activity on human diploid lung fibroblasts compared w ... | 1980 | 6253400 |
| relationship between the increased sensitivity of heat injured clostridium perfringens spores to surface active antibiotics and to sodium chloride and sodium nitrite. | 1980 | 6253431 | |
| regulation of phosphatidylcholine biosynthesis in cultured chick embryonic muscle treated with phospholipase c. | cultures of embryonic chick muscle cells grown in medium containing phospholipase c from clostridium perfringens incorporated [3h]choline into lipid at a rate 3- to 5-fold higher than control cultures. to determine the mechanism by which stimulation of phosphatidylcholine synthesis occurred in phospholipase c-treated cells, activities of enzymes and levels of intermediates in the biosynthetic pathway for phosphatidylcholine were examined. activities of choline kinase, choline phosphotransferase, ... | 1980 | 6253483 |
| hepatitis due to clostridium perfringens in a dog. | 1980 | 6254225 | |
| clostridium perfringens type e enterotoxaemia in rabbits. | 1980 | 6254237 | |
| the isolation of salmonella from jejunal and caecal lymph nodes of slaughtered animals. | one jejunal and one caecal lymph node were sampled from each of 50 cows, 40 yearling cattle, 25 sheep, 20 lambs and 45 pigs after slaughter. salmonella, clostridium perfringens and staphylococcus aureus, all organisms which cause food poisoning in man, were sought by direct plating methods. the samples were also enriched and cultured for salmonella. organisms were cultured from 208 (58%) of the 360 lymph nodes; aerobic plate counts yielded up to 25,000 organisms per gram of tissue, although from ... | 1980 | 6254484 |
| role of coccidia in the occurrence of necrotic enteritis of chickens. | clostridium perfringens type a, eimeria acervulina, and eimeria necatrix were used to produce necrotic enteritis in chickens. the disease was produced in all groups of birds that received feed contaminated with c. perfringens. mortality due to necrotic enteritis was highest (53%) in birds infected with e. acervulina before infection with clostridia. there was a significant difference in mortality rates between birds infected with e. acervulina and birds infected with e. necatrix before infection ... | 1980 | 6254485 |
| [experimental and clinical experiences with the synthetic skin cover of polyvinylalcohol-formaldehyde foam (pva)]. | animal experimental research and clinical experience in polyvinylalcohol formaldehyde foam (pva) as a temporary skin cover: the usefulness of pva as a temporary skin cover of burns and infected wounds was studied in animals. on the back of 20 pigs full thickness burns were produced and wounds were infected, then examined macroscopically, histologically and bacteriologically over a period of 18 days. pva showed a better wound cleansing, a significantly reduced bacterial flora and a more rapid gro ... | 1980 | 6254911 |
| purification and properties of alpha-n-acetylgalactosaminidase from clostridium perfringens. | exo-alpha-n-acetylgalactosaminidase has been purified 8000-fold from clostridium perfringens by gel filtration, ion exchange chromatography, isoelectric precipitation, and negative adsorption on human o type erythrocytes. the resulting enzyme is active at physiological ph and temperature. phenyl glycosides, oligosaccharides, mucins, glycolipids, and cell membranes are substrates for this enzyme. the result of enzyme action on blood type a erythrocytes is the loss of a activity and the simultaneo ... | 1980 | 6254979 |
| clostridium perfringens toxins (type a, b, c, d, e). | 1980 | 6255496 |