Publications

evolution of beta-lactamase inhibitors.	beta-lactamase, the bacterial enzyme that can inactivate penicillins, cephalosporins and related antibiotics, can function outside the cell or in the periplasmic space. this resistance can be transferred between bacteria of the same or different species. most strains of staphylococcus aureus, staphylococcus epidermidis, klebsiella, enterobacter, pseudomonas aeruginosa and bacteroides species are beta-lactamase producers. clavulanic acid is a beta-lactamase inhibitor that works by blocking the en ...	1988	3294401
human antibody responses against schistosomal antigens. i. antibodies from patients with ancylostoma, ascaris lumbricoides or schistosoma mansoni infections react with schistosome antigens.	sera of patients with either hookworm infections or ascariasis, who are from areas nonendemic for schistosoma mansoni, contain antibody reactivity against extracts of s. mansoni schistosomula. such patients with hookworm, but not those with ascaris lumbricoides, also express considerable antibody activity against intact schistosomula and schistosomular membrane preparations. sera from patients with schistosomiasis mansoni also contain these activities. because these three helminthic infections o ...	1988	3128128
the identification and antimicrobial susceptibility testing of neisseria gonorrhoeae, 1980-1987. results from the college of american pathologists microbiology surveys program.	the results of the college of american pathologists (skokie, iii) microbiology surveys from 1980 through 1987 indicate subscriber laboratories have continued to improve their ability to identify and test the antimicrobial susceptibility of neisseria gonorrhoeae. bacteriology and comprehensive surveys participants correctly identified this organism more frequently (95% to 96%) than did the basic surveys laboratories. neisseria gonorrhoeae samples in mixed cultures were slightly more difficult (3% ...	1988	3128961
evidence for thromboxane biosynthesis in established cell lines derived from human lung adenocarcinomas.	thromboxane b2 (txb2) is the stable nonenzymatic hydrolysis product of thromboxane a2, a substance implicated in the initiation of the facilitative role of thrombocytes in the metastatic process. txb2 was isolated from protein-free culture medium of cell lines calu-3, calu-6, a549, and a549/asc-1, derived from human lung adenocarcinomas. txb2 and other 20-carbon fatty acid cyclooxygenase products synthesized from exogenous and endogenous arachidonic acid were identified by their characteristic r ...	1988	3129183
n-0437: a selective d-2 dopamine receptor agonist in in vitro and in vivo models.	the selectivity of the potent dopamine d-2 agonist 2-(n-propyl-n-2-thienylethylamino)-5-hydroxytetralin (n-0437) was examined in a series of in vivo and in vitro pharmacological models. in radioligand binding assays, n-0437 showed high potency (ki = 0.69 nm) and selectivity for d-2 receptors as compared to its potency and selectivity at various other neuronal receptors (ki in nm): d-1 (678) dopamine, alpha 1-(534) and alpha 2-(195) adrenoceptor, s1-(6940) and s2-(5900) serotonin and muscarine (2 ...	1988	3130270
the inhibition of pb125i formation in calf thyroid caused by 14-iodo-15-hydroxy-eicosatrienoic acid is due to decreased h2o2 availability.	previous work from our laboratory has shown that 14-iodo-15-hydroxy-5,8,11-eicosatrienoic acid (i-ho-a) is a potent inhibitor of iodine organification in calf thyroid slices. the present studies were performed in order to clarify the mechanism of this action. incubation of thyroid slices with 10(-4)m i-ho-a caused a 47 and 53% decrease in pb125i formation after 30 and 60 min incubation, respectively. in a series of experiments an inverse relationship between the degree of inhibition caused by i- ...	1988	3131225
j chain in rana catesbeiana high molecular weight ig.	a polypeptide homologous to human and mouse j chain has been identified in the high molecular weight (hmw) ig of the bullfrog, rana catesbeiana. in previous studies, we had detected a component that was similar in size to mammalian j chains and that, relative to l chains, migrated rapidly to the anode in alkaline-urea page; however, its mobility was less than that of mammalian j chains. we now demonstrate that this component is covalently linked to the h chain of r. catesbeiana hmw ig. all of th ...	1988	3131430
[effects of grf 1-29 in normal and hypotrophic lambs].	injections of hgrf rapidly stimulated gh secretion in lamb and this response was significantly higher in hypotrophic than in normal lambs. the treatment had an effect on body composition reducing the retention of lipids and the energy level and increasing calcium and phosphorus content. the results showed that grf affected both metabolism and skeletal growth of ruminants, stimulating specially the production of sm-c and proliferation of chondrocytes.	1988	3131856
treatment of canine embolic pulmonary hypertension with recombinant tissue plasminogen activator. efficacy of dosing regimes.	we investigated effects of two dosing regimes of recombinant tissue plasminogen activator (rt-pa) and sodium heparin on pulmonary thrombolysis in a canine model of pulmonary hypertension, induced by injection of radioactive blood clots. by continuously counting over both lung fields with a mobile gamma camera, we correlated rate and extent of pulmonary thrombolysis with corresponding pulmonary hemodynamics. treatment with heparin, over a 3-hour interval, did not result in significant thrombolysi ...	1988	3133136
micronucleus assay in human fibroblasts: a measure of spontaneous chromosomal instability and mutagen hypersensitivity.	by comparing fibroblast strains derived from individuals exhibiting chromosome instability and/or mutagen hypersensitivity (cockayne syndrome, ataxia telangiectasia, and fanconi anemia) with strains derived from healthy donors, the fibroblast micronucleus assay has been established as a reproducible measure of the genotypic variation in spontaneous or mitomycin c (mmc)-induced chromosomal instability. the patient strains that were moderately or exquisitely sensitive to mmc, whereas the mildly se ...	1988	3133207
strain differences in n-butyl-n-(4-hydroxybutyl)nitrosamine bladder carcinogenesis in rats.	differences in susceptibility of the urinary bladder epithelium to n-butyl-n-(4-hydroxybutyl)-nitrosamine (bbn) in various strains were examined. in experiment 1, 5 strains of male rats were given 0.025% bbn in the drinking water for 8 weeks followed by drinking water without bbn for 32 weeks. analbuminemic rats (nar) and aci rats had high incidences of urinary bladder lesions (papillary or nodular hyperplasia, papilloma and carcinoma), f344 and wistar rats had low incidences, and sprague-dawley ...	1988	3133335
immunocytochemical delineation of thyrotrophic area: origin of thyrotropin-releasing hormone in the median eminence.	to identify the specific thyrotropin-releasing hormone (trh)-containing neurons projecting to the median eminence (me), a retrograde tracing method with horseradish peroxidase (hrp) was combined with immunocytochemical staining for trh. three days after hrp injection restricted to the me, several trh-positive neuronal perikarya were found to contain hrp. such double-stained cells were exclusively distributed in the anterior parts of the periventricular nucleus and the most medial parts of the pa ...	1988	3135505
[dermatoglyphic changes of a familial nature associated with an inversion of the pair 1 chromosomes].		1988	3143141
crystals in calcified epiphyseal cartilage and cortical bone of the rat.	the size of hydroxyapatite (hap) crystals in calcified cartilage and cortical bone of the rat has been studied and compared with that of synthetic poorly crystalline hydroxyapatite (pcha). crystal size was determined by x-ray diffraction and selected-area dark field imaging, and their elemental compositions were determined by emission spectroscopy. the crystal size of cartilage, bone, and pcha were found to be between 120 and 170 a in length by 50 a in width as determined by both x-ray diffracti ...	1988	3145127
interactions between angiotensin ii, sympathetic nerve-mediated pressor response and cyclo-oxygenase products in the pithed rat.	1. the influence of angiotensin ii (aii) on resting blood pressure and on sympathetic nerve-mediated pressor responses in the pithed rat was investigated either by inhibiting the renin-angiotensin system or by infusing aii. 2. plasma aii levels in the pithed rat were approximately 20 fold higher than in normotensive rats. 3. infusion of a subpressor dose of aii (50 ng kg-1 min-1) had no effect on sympathetic nerve mediated pressor responses but a pressor dose of aii, (200 ng kg-1 min-1) facilita ...	1988	3146400
antischistosomal treatment and the measurement of incidence of schistosomiasis in a community of high transmission.	there is disagreement in the measurement of incidence in schistosomiasis concerning which of two categories of negatives is representative of the population of an endemic area: naturally uninfected individuals (untreated negatives) and those made negative following treatment (treated negatives). the incidence of schistosomiasis was studied in the two categories of negatives for two periods among 208 school children (6-11 years old) living in a community of high transmission in zimbabwe. in both ...	1988	3147550
periovulatory serum prolactin and progesteron secretion in gonadotropin stimulated cycles.	in the group of 18 normoprolactinemic patients stimulated by human menopausal gonadotropins (hmg), serum prolactin (prl) and progesteron (p) levels were evaluated at the time of estradiol (e2) increase before, during and after chorionic gonadotropin (hcg) administration. in 8 patients (group i) mean serum prl levels remained normal, ranging from the mean basal values (12.4 +/- 1.7 micrograms/l) to the mean values at the time of hcg administration (16.8 +/- 1.8 micrograms/l) and after it (24.9 +/ ...	1988	3147564
in vivo measurement of intracellular ph in human brain during different tensions of carbon dioxide in arterial blood. a 31p-nmr study.	the effect of changes in carbon dioxide tension in arterial blood upon intracellular ph in brain tissue was studied in seven healthy volunteers, aged 22-45 years. the ph changes were monitored by use of 31p nuclear magnetic resonance spectroscopy, performed on a whole-body 1.5 tesla siemens imaging system. the measurements were carried out during hyperventilation and with the subject breathing atmospheric air containing 5 vol. % and 7 vol. % carbon dioxide. intracellular ph increased significant ...	1988	3147578
chromatographic studies of mitomycin c degradation in albumin microspheres.	serum albumins and polylactic acid (pla) have been used as bioerodable polymers in the preparation of drug-containing microspheres for parenteral drug delivery. the albumin microsphere may be prepared via either chemical cross-linking or heat denaturation of the protein. heat-denatured albumin microspheres containing mitomycin c (mmc) have been used in pre-clinical and clinical investigations. due to the high reactivity of mmc as a bifunctional alkylating agent, a study on the stability of mmc i ...	1988	3148622
characterization of the priming effects of human granulocyte-macrophage colony-stimulating factor on human neutrophil leukotriene synthesis.	human granulocyte-macrophage colony-stimulating factor (gm-csf) is an in vitro and in vivo stimulator of human bone marrow myelomonocytic precursor cells and mature granulocyte and macrophage effector cells. we have compared the effect of gm-csf on the synthesis of 5-lipoxygenase products induced by the chemotactic peptide fmet-leu-phe and the calcium ionophore a23187 in human neutrophils. although gm-csf alone did not stimulate detectable synthesis of products of the 5-lipoxygenase pathway, pre ...	1988	3148964
[lysosomal enzyme activity in the nerve tissue of various species of animals after administration of a neurotoxic pesticide aphos].		1988	3149613
production of prostaglandins and hete-compounds by isolated-cultivated bovine aortic endothelial cells.	the main product of aa conversion by baec is pgi2. other pgs were synthesized by confluent cells only in traces with exception of a hht-like compound which has still to be characterized in more details. a significant release of hete was only detected when baec were destroyed. under these conditions about 1 pmole/10(5) cells 15- and 12 hete were released by baec. taken into account the amounts of hete produced by endothelial cells as well as the partially contradictory activities of 12- and 15-he ...	1988	3150276
sodium valproate and chlordiazepoxide in the elevated plus-maze test of anxiety in the rat.	the effects of sodium valproate (25-200 mg/kg i.p.) were investigated in the elevated plus-maze test of anxiety in the rat. no single dose significantly increased the percentage of entries made onto the open arms. chlordiazepoxide (5 mg/kg) both acutely and after 5 days of administration significantly increased the percentage of entries onto open arms and this anxiolytic effect was neither potentiated, nor antagonised, by sodium valproate (25-200 mg/kg). chlordiazepoxide also increased the total ...	1988	3151011
an nmr blood test for cancer: a critical assessment.	an evaluation of the reproducibility and accuracy of the nmr human blood test for cancer described by fossel, e. t., carr, j. m. and mcdonagh, j., (new england journal of medicine 315, 1369-1376) in 1986 has been conducted jointly at the national cancer institute-frederick cancer research facility, frederick, md (nci-fcrf) and the national research council, ottawa, canada (nrc). the influences on the test of the following were studied: (a) subject fasting; (b) sample collection, storage and hand ...	1988	3079310
an nmr blood test for cancer: a critical assessment.	an evaluation of the reproducibility and accuracy of the nmr human blood test for cancer described by fossel, e. t., carr, j. m. and mcdonagh, j., (new england journal of medicine 315, 1369-1376) in 1986 has been conducted jointly at the national cancer institute-frederick cancer research facility, frederick, md (nci-fcrf) and the national research council, ottawa, canada (nrc). the influences on the test of the following were studied: (a) subject fasting; (b) sample collection, storage and hand ...	1988	3079310
ethical aspects of military physicians treating patients with hiv/part two: the duty to take initiative.		1988	3126439
identification of encapsulated and non-encapsulated yersinia pestis by immunofluorescence tests using polyclonal and monoclonal antibodies.	rabbit polyclonal hyperimmune antibodies to yersinia pestis, and a mouse monoclonal antibody against the capsular antigen fraction 1 (f1) were compared in immunofluorescence (if) tests. fluorescent antibody conjugates were prepared from polyclonal antisera to four f1 positive y. pestis strains; the conjugated antibody to strain a1122 gave the strongest if staining of f1 positive and f1 negative y. pestis strains. indirect assays were rejected in favour of direct assays utilizing polyclonal and m ...	1988	3042439
increased virulence of yersinia pseudotuberculosis by two independent mutations.	a chromosomally encoded protein, which mediates invasion into hela cells was recently identified in yersinia pseudotuberculosis. the role of this protein (invasin) in the virulence process was not, however, investigated. we show that mutation of the invasin gene in y. pseudotuberculosis abolishes the ability of the bacteria to invade hela cells. when mice were challenged by intraperitoneal injection both the mutant and the wild-type strain produced infections of similar virulence but mutant show ...	1988	3043229
[use of quantitative immunofluorescence for determining the adsorption capacity of magnetic immunosorbents].	the adsorption capacity of microgranulated polyacrylamide magnetic immunosorbents has been studied by the method of quantitative immunofluorescence as applied to the causative agents of plague, cholera, and melioidosis. similar regularity in the dynamics of antigen adsorption on the granules of magnetic immunoadsorbents has been established. this regularity consists in the direct relationship between this process and the concentration of infective agents interacting with magnetic sorbents.	1988	3046196
in vitro expression of a 22-kilodalton yersinia pestis polypeptide immunologically related to the 25-kilodalton plasmid-encoded protein of the three pathogenic yersinia species.	antibodies raised against the 25-kilodalton (p25) plasmid-encoded polypeptide of yersinia enterocolitica recognized the homologous protein in the three yersinia species grown in vitro. this polypeptide was recovered from whole cells as well as from the fluid supernatant of bacteria grown at 37 degrees c in a ca2+-deficient medium. furthermore, a 22-kilodalton (p22) plasmid-encoded polypeptide immunologically related to p25 was found only in y. pestis during early growth. after 30 h of culture, t ...	1988	3047061
immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis.	the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ...	1988	3047063
immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis.	the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ...	1988	3047063
effects of estrogen on hypothalamic gonadotropin-releasing hormone release in castrated male rhesus macaques.	the evidence that hypothalamic gonadotropin-releasing hormone (gnrh) release increases during the estrogen-induced luteinizing hormone (lh) surge in castrated female macaques and that estrogen induces a similar lh surge in the male suggests that either sexual differentiation of gnrh secretion does not exist or that changes in brain gnrh are not critical for ovulation. we tested this hypothesis by using the push-pull perfusion (ppp) technique to monitor gnrh release in the mediobasal hypothalamus ...	1988	3048539
in vitro and ex vivo enhancement of nonspecific phagocytosis by cefodizime.	the in vitro and ex vivo effects of cefodizime on some functional activities of both human neutrophils and monocytes were studied. in vitro experiments were performed with antibiotic concentrations ranging from 1 to 200 micrograms/ml. for the ex vivo study, 7 adult healthy controls were treated intravenously with 4 g/day of cefodizime for 6 days. we found that the drug modulated phagocytosis frequency and index when nonopsonized zymosan and heat-killed candida albicans were used as phagocytic ch ...	1988	3053065
in vitro and ex vivo enhancement of nonspecific phagocytosis by cefodizime.	the in vitro and ex vivo effects of cefodizime on some functional activities of both human neutrophils and monocytes were studied. in vitro experiments were performed with antibiotic concentrations ranging from 1 to 200 micrograms/ml. for the ex vivo study, 7 adult healthy controls were treated intravenously with 4 g/day of cefodizime for 6 days. we found that the drug modulated phagocytosis frequency and index when nonopsonized zymosan and heat-killed candida albicans were used as phagocytic ch ...	1988	3053065
the plasmid-encoded outer-membrane proteins of yersinia pestis.	the yersinial plasmid-encoded outer-membrane proteins (yops) are encoded by the low-ca++ response virulence plasmid present in the yersiniae pathogenic for humans. it has been shown that in yersinia pestis kim there are 11 of these proteins, which are expressed maximally during growth at 37 degrees c in the absence of ca++. to recover these proteins in outer membranes, it was necessary to transfer the y. pestis low-ca++ response plasmid to yersinia pseudotuberculosis. however, two of these prote ...	1988	3055200
the bubonic plague.	in a.d. 1346 some 100 million people inhabited europe, northern africa and the near east. five years later 25 million were dead--victims of the black death. the plague kept reappearing, but the epidemics did not spread as widely: apparently a new and milder strain of yersinia pestis evolved that made at least some people immune to the virulent strain.	1988	3055286
immunomodulators.	a new era is emerging in the therapy of rheumatic disease involving the use of biologically based immunomodulators. cytokines, such as the interleukins and interferons, are proteins produced by activated lymphoid cells which can now be synthesised by genetic engineering techniques. these agents have already been used to correct "immune deficiencies" in patients with neoplastic and inflammatory disease e.g. gamma-interferon in rheumatoid arthritis (ra). monoclonal antibodies (mca) targeted agains ...	1988	3055878
d-arabinose metabolism in escherichia coli b: induction and cotransductional mapping of the l-fucose-d-arabinose pathway enzymes.	d-arabinose is degraded by escherichia coli b via some of the l-fucose pathway enzymes and a d-ribulokinase which is distinct from the l-fuculokinase of the l-fucose pathway. we found that l-fucose and d-arabinose acted as the apparent inducers of the enzymes needed for their degradation. these enzymes, including d-ribulokinase, appeared to be coordinately regulated, and mutants which constitutively synthesized the l-fucose enzymes also constitutively synthesized d-ribulokinase. in contrast to d ...	1988	3056899
[comparative study of ribosomal proteins from yersinia pestis and escherichia coli: amino acid composition and electrophoretic mobility].	the techniques of electrophoresis in polyacrylamide gel and amino acid analysis permitted to find the slight difference in the composition of ribosomal proteins from yersinia pestis and escherichia coli cells. ribosomal proteins were mapped and classified on the basis of two-dimensional electrophoresis data. protein "x" was registered in the total ribosomal protein due to its separation in course of ribosomal subunits dissociation.	1988	3057358
infection as a complication of heart transplantation.	infection and cardiac rejection are the most significant causes of morbidity and mortality after heart transplantation. at some transplant centers, more than half of the early transplantation-related deaths are the result of infection. the infectious agents may be transmitted to the host by means of the allograft, through blood transfusion, by nosocomial or environmental routes, or they may represent endogenous microbial flora or reactivation of a prior infection. the frequency of infectious com ...	1988	3058911
identification and localization of a novel, cytoskeletal, centrosome-associated protein in ptk2 cells.	antisera raised against centrin (salisbury, j.l., a.t. baron, b. surek, and m. melkonian. 1984. j. cell biol. 99:962-970) have been used, here, to identify a centrosome-associated protein with an mr of 165,000. immunocytochemistry indicates that this protein is a component of pericentriolar satellites, basal feet, and pericentriolar matrix of interphase cells. these components of pericentriolar material are, in part, composed of 3-8-nm-diam filaments, which interconnect to form a three-dimension ...	1988	3060471
[characteristics of the process of transferrin iron utilization by yersinia pestis at various incubation temperatures].	the study has revealed that for the utilization of iron contained in transferrin the direct contact of y. pestis with this metalloprotein is necessary. at 28 degrees c y. pestis utilizes iron contained in transferrin. at 37 degrees c y. pestis absorbs transferrin, but cannot utilize its iron, which is probably linked with disturbances in the system of the transfer of iron from the transferrin receptor complex into the bacterial cell.	1988	3061259
antipeptide monoclonal antibodies inhibit the binding of rabies virus glycoprotein and alpha-bungarotoxin to the nicotinic acetylcholine receptor.	it has been reported that binding to muscle nicotinic acetylcholine receptor at the post-synaptic membrane is an important event of the rabies virus neurotropism. the binding site can be located within the 190-203 region of the virus glycoprotein sharing a high degree of homology with the "toxic loop" of the curare-mimetic snake neurotoxins. we have synthesized a tetradecapeptide corresponding to this glycoprotein region and used it, following conjugation with an immunogenic carrier to raise mab ...	1988	3062388
[the results of using an immunoenzyme method in an epizootiologic survey of natural siberian foci of plague].	the approbation of the enzyme immunoassay in the study of diverse field material (corpses of animals, ectoparasites, soil, feces of birds of prey, etc.) for the presence of yersinia pestis contamination has shown the advantages of this assay over the existing serological tests: the passive hemagglutination test and the antigen (or antibody) neutralization test. the use of the immunoperoxidase preparation obtained on the basis of staphylococcal protein a makes it possible to detect antibodies to ...	1988	3064503
genetic analysis of human malignancy using somatic cell hybrids and monochromosome transfer.	evidence is presented for the existence of genes which regulate the malignant behaviour of human cancer cells. observations obtained from somatic cell hybrid and monochromosome transfer studies suggest that recessive genes play an important part in the control of malignant expression. loss of restriction fragment length polymorphism alleles, consistent with loss of these putative tumour-suppressor genes, is associated with an increasing number of human cancers.	1988	3066478
quality analysis of dsa equipment.	in the framework of a quality analysis project for the improvement of digital subtraction angiography (dsa) equipment, an inventory was made of the image quality and radiation dose of dsa equipments in six hospitals in the netherlands. the image quality was investigated with a contrast detail (cd) phantom. the entrance dose of the radiation on this phantom and the skin dose at the level of the eye lenses and the thyroid gland were measured in these hospitals using a human phantom during a standa ...	1988	3067118
quality analysis of dsa equipment.	in the framework of a quality analysis project for the improvement of digital subtraction angiography (dsa) equipment, an inventory was made of the image quality and radiation dose of dsa equipments in six hospitals in the netherlands. the image quality was investigated with a contrast detail (cd) phantom. the entrance dose of the radiation on this phantom and the skin dose at the level of the eye lenses and the thyroid gland were measured in these hospitals using a human phantom during a standa ...	1988	3067118
inhibition of the intraerythrocytic development of plasmodium falciparum in glucose-6-phosphate dehydrogenase deficient erythrocytes is enhanced by oxidants and by crisis form factor.	in this study, we have examined p. falciparum development in untreated normal or g6pd deficient erythrocytes as well as in parasitized cells exposed to isouramil--a fava bean extract known to induce oxidant stress in g6pd deficient erythrocytes (a condition known as favism), to diamide--a thiol oxidizing agent and to crisis form factor (cff)--a non-immunoglobulin product found in the sera of man immune to malaria. we observed a significant retardation in intraerythrocytic development of parasite ...	1988	3067318
ureaplasma urealyticum. overview with emphasis on fetal and maternal infections.		1988	3067640
preparation of yersinia pestis plague murine toxin.		1988	3068484
histochemical, immunofluorescence, and ultrastructural differences in fetal cartilage among three genetically distinct chondrodystrophic mice.	the severe lethal chondrodystrophies in man result in a common clinical syndrome including shortening of the face, mandible, and limbs. studies of three lethal chondrodystrophic mutants in mice, viz., chondrodysplasia (cho), cartilage matrix deficiency (cmd), and disproportionate micromelia (dmm), which share this syndrome, were performed with the aim of identifying histochemical, immunofluorescence, or ultrastructural differences which might exist among these hereditary cartilage disorders. we ...	1988	3070812
histochemical, immunofluorescence, and ultrastructural differences in fetal cartilage among three genetically distinct chondrodystrophic mice.	the severe lethal chondrodystrophies in man result in a common clinical syndrome including shortening of the face, mandible, and limbs. studies of three lethal chondrodystrophic mutants in mice, viz., chondrodysplasia (cho), cartilage matrix deficiency (cmd), and disproportionate micromelia (dmm), which share this syndrome, were performed with the aim of identifying histochemical, immunofluorescence, or ultrastructural differences which might exist among these hereditary cartilage disorders. we ...	1988	3070812
occurrence of unusual manifestations and uncommon serotypes of salmonella in human infection.		1988	3071548
occurrence of unusual manifestations and uncommon serotypes of salmonella in human infection.		1988	3071548
[immunoenzyme analysis using paper disks].	filter paper discs have been used in the enzyme immunoassay (eia) as solid phase instead of polystyrene plates. the use of paper discs has made it possible to achieve a multiple increase in the sensitivity of sandwich eia, thus permitting the detection of yersinia pestis capsular antigen at a concentration of 0.4 ng/ml. paper discs can be used not only for the sorption of antigen and antibodies, but also for the affinity purification of preparations.	1988	3072810
extracellular matrix of the human retinal pigment epithelial cells in vitro.		1988	3073234
localization of the kras2 oncogene in the domestic rabbit (oryctolagus cuniculus).	the kras2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3h-labeled human cdna probe. there were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of kras2 to 16p11----q11. in additi ...	1988	3073912
[effect of the cultivation temperature on the physicochemical properties of the dna-dependent rna-polymerase of yersinia pestis].		1988	3074242
food intake of infant rats after intraperitoneal administration of insulin.	the effects of intraperitoneal insulin on the food intake have been determined in infant rats up to weaning. it was found that intraperitoneal (ip) insulin reduced the milk intake of 13 and 17 day-old pups for three hours after treatment. in 5, 9 and 24 day-old pups the food intake was not significantly changed after ip insulin administration. only in 28 day-old rat pups ip insulin induced an increase of food intake. since subcutaneously (sc) administered insulin gave rise to short-term hyperpha ...	1988	3075555
food intake of infant rats after intraperitoneal administration of insulin.	the effects of intraperitoneal insulin on the food intake have been determined in infant rats up to weaning. it was found that intraperitoneal (ip) insulin reduced the milk intake of 13 and 17 day-old pups for three hours after treatment. in 5, 9 and 24 day-old pups the food intake was not significantly changed after ip insulin administration. only in 28 day-old rat pups ip insulin induced an increase of food intake. since subcutaneously (sc) administered insulin gave rise to short-term hyperpha ...	1988	3075555
estrogenic control of spermidine/spermine n1-acetyltransferase activity in rat uterus.		1988	3076330
effect of live and heat-killed bacteria on the secretory activity of paneth cells in germ-free mice.	germ-free mice were given live or heat-killed facultative anaerobes, and the ultrastructure of ileal paneth cells was quantitatively examined with special reference to secretory granules showing a bipartite substructure (central core and peripheral halo). after administering live or heat-killed bacteria, there was a decrease in the area occupied by the cores of secretory granules in paneth cells, and exocytosed core material was observed in the crypt lumen. there were no changes in the area occu ...	1988	2963697
systolic and diastolic dysfunction during atrial pacing in conscious dogs with left ventricular hypertrophy.	to determine the extent to which the hypertrophied left ventricle responds to the chronotropic stress induced by graded atrial pacing rates, we studied conscious, chronically instrumented dogs with severe compensated pressure overload left ventricular (lv) hypertrophy induced by aortic banding in puppies 8-10 weeks of age. at 1-2 years, dogs with severe lv hypertrophy (lv free wall/body wt ratio 6.8 +/- 0.6 g/kg) and sham-operated littermates (lv free wall/body wt ratio 4.0 +/- 0.3 g/kg) were in ...	1988	2963705
125i-d(ch2)5[tyr(me)2, tyr(nh2)9]avp: iodination and binding characteristics of a vasopressin receptor ligand.	a radioiodinated vasopressin antagonist, d(ch2)5[tyr(nh2)9]avp has been prepared. iodination was carried out at the phenyl moiety of the tyrosylamide residue at position 9, followed by hplc purification. non-radiolabelled monoiodinated antagonist was used as a reference for identification. 125i-d(ch2)5[tyr(me)2, tyr(nh2)9]avp binding appeared to take place with a dissociation constant of 0.28 +/- 0.09 nm (kd +/- sd) to v1 vasopressin receptors on rat liver membranes.	1988	2964381
evidence for in vitro regulation of ige receptors on the human basophil membrane by their removal and reexpression: effects of ca2+, mg2+, some metabolic inhibitors and fetal calf serum.	human basophils free of receptor-bound ige and suspended in rpmi-1640 + 0.2% edta or in a buffered salt solution lacking ca2+ and mg2+ bound optimal quantities of 125i ige. in media containing ca2+ and mg2+ such as rpmi-1640 alone or a buffered physiologic salt solution binding was reduced significantly in the first 60 min and remained essentially unchanged in the next 120 min. scatchard analysis showed the reduction to be due both to loss of number and affinity of receptors. the presence of bot ...	1988	2965111
helper t-cell clones that recognize autologous insulin are stimulated in nonresponder mice by pork insulin.	murine antibody responses to various species of insulin are under major histocompatibility complex-linked ir gene control. beef insulin differs from pork insulin by only two amino acids in the a-chain loop, yet strain c57bl/10 (b10) mice produce insulin-specific antibodies after immunization with beef insulin and fail to produce antibody after stimulation with pork insulin. nevertheless, pork insulin primes helper t cells in b10 mice that can be demonstrated if insulin-specific lyt-1-, -2+ suppr ...	1988	2965814
[antigenuria in plague-infected great gerbils].	in the urine of plague-infected great gerbils yersinia pestis capsular antigen was detected by means of diagnostic preparations, both commercial and experimental (based on monoclonal antibodies). the antigen was detected in many urine samples taken from the animals over a prolonged period. the incidence and duration of antigenuria were found to be related to the survival time of great gerbils after infection and the level of antibodies in their blood. the number of animals with antigenuria marke ...	1988	2966529
homology of glucosyltransferase gene and protein sequences from streptococcus sobrinus and streptococcus mutans.	the sequences of glucosyltransferase genes from streptococcus sobrinus (gtfi) and streptococcus mutans (gftb) were compared and show a high degree of homology. there is a 57.7% homology of nucleotides in the genes and a 56.7% homology of amino acids in the deduced protein sequences. the g + c content for the protein-coding region is 43.6% for s. sobrinus and 41.2% for s. mutans. internal repeating sequences present in both proteins exhibit some difference in sequence pattern.	1988	2971696
[antagonistic and insecticidal properties of soil micromycetes].	it was shown that microscopic fungi isolated in plague foci had an antagonistic effect on the plague causative agent. in the majority of the cases it was associated with their insecticidal activity against plague-transmitting fleas. it is expedient to consider the relations revealed between the burrow biocenosis components in investigation of plague enzootic aspects and development of new biological insecticides for control of the infection carriers.	1988	2973774
transcriptional mapping and nucleotide sequence of the escherichia coli fepa-fes enterobactin region. identification of a unique iron-regulated bidirectional promoter.	the iron-controlled fepa and fes-entf transcripts from the escherichia coli enterobactin gene complex are expressed divergently from a limited genetic region, thereby suggesting the existence of a single, possibly overlapping promoter junction for these genes. the nucleotide sequence of a 1,997-base pair hpai fragment specific for this genetic region allowed for the identification of an 1,122-base pair open reading frame as the previously uncharacterized fes gene. its product, fes (approximately ...	1988	2974033
the relationships between oligomeric structure and function of band 3 protein from human erythrocyte membranes: present knowledge and suggestions for further experiments.		1988	2976450
regional levels of neurotransmitter markers in the pigeon telencephalon: a comparison with possibly homologous areas of the rat telencephalon.	the levels of cholinergic, gamma-aminobutyric acidergic (gabaergic), and excitatory amino acid neurotransmitter markers have been measured in 18 regions of the pigeon telencephalon as well as in supposedly homologous areas of the rat telencephalon. among the basal telencephalic areas, some similar patterns of regional distribution were observed, with the noticeable exception of the ratio of levels of cholinergic markers between the striatum and globus pallidus, which was much larger in the rat t ...	1988	2897425
dextrorphan and dextromethorphan, common antitussives, are antiepileptic and antagonize n-methyl-d-aspartate in brain slices.	the antitussive, dextromethorphan (dm), and its metabolite, dextrorphan (dx), were evaluated for antiepileptic properties in vitro. interictal bursts and prolonged ictal epileptiform afterdischarges, induced by perfusion of guinea pig neocortical brain slices with mg2+-free solution, were blocked by dx (1-250 microm) or dm (100 microm). intracellular records showed that these agents blocked n-methyl-d-aspartate (nmda)-induced depolarizations without altering intrinsic membrane properties. dx blo ...	1988	2897648
the prevention and avoidance of genetic disease: summing up.	the preceding papers have dealt with major advances in understanding and detecting the mutational basis of human disease. if these advances are to be of practical benefit, systems of effective, efficient and acceptable delivery of the technology to the relevant population groups will need to be planned. in these delivery systems, the key figure is likely to be the clinical geneticist, still a somewhat shadowy figure, difficult to define: a doctor among scientists, and a scientist among doctors. ...	1988	2900529
quantitation of pancuronium, 3-desacetylpancuronium, vecuronium, 3-desacetylvecuronium, pipecuronium and 3-desacetylpipecuronium in biological fluids by capillary gas chromatography using nitrogen-sensitive detection.	a sensitive and specific capillary gas chromatographic (gc) assay was developed for the quantitation of the quaternary ammonium steroidal neuromuscular blocking drugs pancuronium (panc), vecuronium (vec) and pipecuronium (pip), as well as the metabolites 3-desacetylpancuronium (3-despanc) and 3-desacetylvecuronium (3-des vec) in plasma, bile and urine; the putative metabolite 3-desacetylpipecuronium (3-des pip) was extracted and quantitated only in urine. the procedure employed a single dichloro ...	1988	2900840
atp-dependent glutamate uptake into synaptic vesicles from cerebellar mutant mice.	the atp-dependent glutamate uptake system in synaptic vesicles prepared from mouse cerebellum was characterized, and the levels of glutamate uptake were investigated in the cerebellar mutant mice, staggerer and weaver, whose main defect is the loss of cerebellar granule cells, and the nervous mutant, whose main defect is the loss of purkinje cells. the atp-dependent glutamate uptake is stimulated by low concentrations of chloride, is insensitive to aspartate, and is inhibited by agents known to ...	1988	2901460
responses of hippocampal pyramidal cells to putative serotonin 5-ht1a and 5-ht1b agonists: a comparative study with dorsal raphe neurons.	in low cerveau isolé transected rats, the effects of microiontophoretic application of putative serotonin 5-ht1a and 5-ht1b agonists on the spontaneous firing rate of ca1 pyramidal cells were compared to those of 5-ht. in contrast to the large current-dependent suppression of unit activity observed with 5-ht, the 5-ht1a compounds, ipsapirone, 8-oh-dpat (8-hydroxy-2-(di-n-propylamino)-tetralin) and ly 165163 (p-aminophenylethyl-m-trifluoromethylphenylpiperazine) and the 5-ht1b compounds, mcpp (m- ...	1988	2901680
measurement of dynorphins with 3h-etorphine and canine limbic system receptors.	a canine limbic system preparation was used as the source of opioid peptide receptors to screen biologic extracts for the presence of opioid receptoractive peptides following their gradient rp-hplc separation. eight synthetic dynorphin peptides were studied for their ability to displace the commonly-used ligand 3h-etorphine from the canine limbic system p2 preparation. the peptides studied included the dynorphins 1-7, 1-8, 1-9, 1-10, 1-12, 1-13, 1-17, and dynorphin b. two different types of opio ...	1988	2901686
clinical and serological features of human herpesvirus-6 infection in three adults.	3 adult patients with serological evidence of human herpesvirus-6 (hhv-6) infection had mild, afebrile illnesses with nonspecific symptoms. in each case, the characteristic clinical feature was the presence of enlarged, bilateral, non-tender, anterior and posterior cervical nodes early in the illness which persisted for up to 3 months. hhv-6 igg antibody reciprocal titres of 160 to 2560 were found during acute infection, and decreased to a titre of 10 in 1 patient 3 years later. igm responses we ...	1988	2902266
c-erba protooncogenes mediate thyroid hormone-dependent and independent regulation of the rat growth hormone and prolactin genes.	regulation of gene expression by the thyroid hormones is thought to be mediated by a nuclear-associated receptor found in a wide variety of cells and tissues. cellular homologues of the avian erythroblastosis virus oncogene, v-erba, encode proteins which bind thyroid hormone with similar affinities as thyroid hormone receptors. however, it has not been shown that any of the c-erba proteins can function as receptor and modulate thyroid hormone responsive genes. in this study, using transient expr ...	1988	2903439
foot trauma due to rodents in sierra leone.		1988	2904187
[pili (fimbriae) of the causative agents of plague and pseudotuberculosis: their detection in the hemagglutination reaction, the passive hemagglutination reaction and in an immunoenzyme analysis system].	newly developed serological methods for the detection of pili in the passive hemagglutination (pha) test with the use of immunoglobulin erythrocytic diagnosticum and in the enzyme immunoassay (eia) with the use of specific immunoglobulins labeled with horse radish peroxidase have been found to exceed the method of detecting pili, based on the determination of their hemagglutinating activity, in sensitivity and specificity. besides, the pha test and eia have proved to be capable of detecting low ...	1988	2904200
ca2+-dependent and ca2+-independent glutamate release, energy status and cytosolic free ca2+ concentration in isolated nerve terminals following metabolic inhibition: possible relevance to hypoglycaemia and anoxia.	hypoglycaemia and anoxia both cause massive release of glutamate from the brain in vivo, and the nature of this release was investigated using guinea-pig cerebral-cortical synaptosomes and iodoacetate and rotenone to simulate the energetic consequences of these conditions. glutamate release (by continuous fluorimetry), cytoplasmic free ca2+ (by fura-2), membrane potentials, atp, adp and creatine phosphate were determined in parallel, following the addition of iodoacetate or rotenone, alone or in ...	1988	2904664
[effects of prazosin, sgb-1534, dobutamine and isoproterenol on congestive heart failure in dogs].	effects of prazosin, dobutamine, isoproterenol and sgb-1534, a new alpha-blockade, on congestive heart failure (chf) in dogs were investigated. the model was made by protease injection into the left ventricular free wall, saline loading, and dextran and methoxamine infusions. by this maneuver, left atrial pressure (lap), systemic vascular resistance (svr) and left ventricular end-diastolic pressure (lvedp) were markedly increased, aortic blood flow (aobf) was decreased, and systemic blood pressu ...	1988	2906315
regional localization of the autosomal dominant polycystic kidney disease locus.	the localization of the autosomal dominant polycystic kidney disease locus (pkd1) within an array of anonymous polymorphic dna sequences on chromosome 16 band p13 was determined by multipoint mapping. nine polymorphic dna markers, including two hypervariable sequences, were used to study 19 pkd1 and 21 reference families. pkd1 was found to lie proximal to the 3' and 5' hypervariable regions of alpha-globin and distal to the anonymous sequence cri-0327. somatic cell hybrid mapping places pkd1 wit ...	1988	2906325
genetic analysis of the 9.5-kilobase virulence plasmid of yersinia pestis.	the 9.5-kilobase plasmid of yersinia pestis determines plasminogen activator, coagulase, pesticin, and pesticin immunity activities. we have mapped and cloned the loci encoding these activities and demonstrated that both plasminogen activator and coagulase were determined by the same gene, designated pla. the primary translation product of this gene (38 kilodaltons [kda]) was processed in two sequential steps to produce peptides of 37 and 35 kda. the first step in this processing occurred rapidl ...	1988	2843470
plasminogen activator/coagulase gene of yersinia pestis is responsible for degradation of plasmid-encoded outer membrane proteins.	the related family of virulence plasmids found in the three major pathogens of the genus yersinia all have the ability to encode a set of outer membrane proteins. in y. enterocolitica and y. pseudotuberculosis, these proteins are major constituents of the outer membrane when their synthesis is fully induced. in contrast, they have been difficult to detect in y. pestis. it has recently been established that y. pestis does synthesize these proteins, but that they are rapidly degraded due to some a ...	1988	2843471
detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration.	the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ...	1988	2844405
efficacy of a combination thromboxane receptor antagonist and lipoxygenase inhibitor in traumatic shock.	the effects of a thromboxane receptor antagonist having lipoxygenase inhibitory activity, l-655,240 (3-[1-(4-chlorobenzyl)-5-fluoro-3-methyl-indol-2-yl]2,2-dimethylpropa noic acid) (1 mg/kg per h) were studied in a standardized model of traumatic shock. pentobarbital (35 mg/kg) anesthetized rats subjected to noble-collip drum trauma were characterized by a 82 +/- 12 min survival time, a 20-fold increase in plasma cathepsin d activity, and a 6-fold increase in plasma myocardial depressant factor ...	1988	2845544
efficacy of a combination thromboxane receptor antagonist and lipoxygenase inhibitor in traumatic shock.	the effects of a thromboxane receptor antagonist having lipoxygenase inhibitory activity, l-655,240 (3-[1-(4-chlorobenzyl)-5-fluoro-3-methyl-indol-2-yl]2,2-dimethylpropa noic acid) (1 mg/kg per h) were studied in a standardized model of traumatic shock. pentobarbital (35 mg/kg) anesthetized rats subjected to noble-collip drum trauma were characterized by a 82 +/- 12 min survival time, a 20-fold increase in plasma cathepsin d activity, and a 6-fold increase in plasma myocardial depressant factor ...	1988	2845544
heterogeneity of glycine receptors and their messenger rnas in rat brain and spinal cord.	messenger rnas isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. the fractions were injected into xenopus oocytes to determine their potencies for expression of glycine receptors (glyrs), which were then examined electrophysiologically. the sedimentation profiles disclosed two classes of glyr mrnas, one heavy and the other light. the adult spinal cord was rich in heavy glyr mrna, whereas the light glyr mrna was more abundant in neonatal spinal cord and in ad ...	1988	2845580
characterization of vaccinia virus dna topoisomerase i expressed in escherichia coli.	the putative structural gene encoding the vaccinia virus type i dna topoisomerase (ec 5.99.1.2) was expressed in escherichia coli under the control of a bacteriophage t7 promoter. provision of t7 rna polymerase resulted in the accumulation to high level of a mr = 33,000 type i topoisomerase with the properties of the vaccinia enzyme. a simple purification scheme yielded approximately 8 mg of recombinant vaccinia topoisomerase from 400 ml of bacteria. dna unwinding by the enzyme was stimulated by ...	1988	2846543
characterization of vaccinia virus dna topoisomerase i expressed in escherichia coli.	the putative structural gene encoding the vaccinia virus type i dna topoisomerase (ec 5.99.1.2) was expressed in escherichia coli under the control of a bacteriophage t7 promoter. provision of t7 rna polymerase resulted in the accumulation to high level of a mr = 33,000 type i topoisomerase with the properties of the vaccinia enzyme. a simple purification scheme yielded approximately 8 mg of recombinant vaccinia topoisomerase from 400 ml of bacteria. dna unwinding by the enzyme was stimulated by ...	1988	2846543
infection control measures for human immunodeficiency virus (hiv): universal precautions.		1988	2846803
[effect of bilirubin and serum albumin on the na,k-atpase activity in the synaptosomal membrane].	the absorption spectrum of visible light, characteristic of the free bilirubin being in the aqueous medium, with a single maximum at 440 nm and with the shoulder in the region of 410-420 nm is transformed into the spectrum with two maxima in the region of 460 and 500 nm, respectively, when the pigment is bound in vitro by the synaptosomal membrane. there are two types of sites for bilirubin binding in the membrane particles, differing in the values of constants of association (ka = 0.6 . 10(5) a ...	1988	2847380
influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines.	sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ...	1988	2847397
activities of amikacin, roxithromycin, and azithromycin alone or in combination with tumor necrosis factor against mycobacterium avium complex.	the effect of amikacin and two new macrolides (roxithromycin and azithromycin) used either alone or in combination with recombinant tumor necrosis factor (tnf) to inhibit or kill mycobacterium avium complex in human macrophages was examined in vitro. macrophage monolayers infected with m. avium complex (strain 101, serotype 1) were treated with antibiotics or tnf by using three different protocols: (i) antibiotics or tnf was added to the monolayers immediately after infection and washed out afte ...	1988	2847644
activities of amikacin, roxithromycin, and azithromycin alone or in combination with tumor necrosis factor against mycobacterium avium complex.	the effect of amikacin and two new macrolides (roxithromycin and azithromycin) used either alone or in combination with recombinant tumor necrosis factor (tnf) to inhibit or kill mycobacterium avium complex in human macrophages was examined in vitro. macrophage monolayers infected with m. avium complex (strain 101, serotype 1) were treated with antibiotics or tnf by using three different protocols: (i) antibiotics or tnf was added to the monolayers immediately after infection and washed out afte ...	1988	2847644
activation of calpain i and calpain ii: a comparative study using terbium as a fluorescent probe for calcium-binding sites.	the present study demonstrates the activation of calpain i and calpain ii by micromolar levels of terbium and has utilized the enhancement in the fluorescence of protein-bound terbium to study and compare the calcium binding sites of the two enzymes. calpain i and calpain ii were isolated from bovine erythrocytes and brain, respectively. while the rates of activation of calpain i by terbium and calcium are comparable, the rate of activation of calpain ii was much greater in the presence of terbi ...	1988	2847653