Publications

nucleotide sequence of the virulence gene virg of the agrobacterium tumefaciens octopine ti plasmid: significant homology between virg and the regulatory genes ompr, phob and dye of e. coli.	the entire nucleotide sequence of the virg locus, from the octopine ti plasmid of agrobacterium tumefaciens strain 15955, has been determined. the virg gene is 801 nucleotides in length and has one open reading frame which encodes a protein of mr 29,995. the virg gene is involved in the transcriptional activation of the ti plasmid vir-loci, which occurs after induction by specific compounds present in plant exudate. sequence analysis of the agrobacterium virg protein showed significant homology ...	1986	3027669
the effects of electroconvulsive therapy and antidepressant drugs on monoamine receptors in rodent brain--similarities and differences.	repeated administration to rodents of electroconvulsive shock (ecs) produces changes in brain monoamine biochemistry and function, several of which are also seen after repeated administration of antidepressant drugs. both repeated ecs and antidepressant drug administration decrease cortical beta-adrenoceptor density and attenuate the alpha 2-adrenoceptor-mediated sedation response to clonidine injection. neither procedure alters phenylephrine-induced locomotor activity in mice, a measure of alph ...	1986	3028726
the effects of electroconvulsive therapy and antidepressant drugs on monoamine receptors in rodent brain--similarities and differences.	repeated administration to rodents of electroconvulsive shock (ecs) produces changes in brain monoamine biochemistry and function, several of which are also seen after repeated administration of antidepressant drugs. both repeated ecs and antidepressant drug administration decrease cortical beta-adrenoceptor density and attenuate the alpha 2-adrenoceptor-mediated sedation response to clonidine injection. neither procedure alters phenylephrine-induced locomotor activity in mice, a measure of alph ...	1986	3028726
reconstitution of vesicle fusions occurring in endocytosis with a cell-free system.	we have used defined subcellular fractions to reconstitute in a cell-free system vesicle fusions occurring in the endocytic pathway. the endosomal fractions were prepared by immuno-isolation using as antigen an epitope located on a foreign protein, the transmembrane glycoprotein g (g-protein) of vesicular stomatitis virus. the g-protein was first implanted in the cell plasma membrane and subsequently endocytosed for 15 to 30 min at 37 degrees c. the endosomal fractions were immuno-isolated on a ...	1986	3028771
reconstitution of vesicle fusions occurring in endocytosis with a cell-free system.	we have used defined subcellular fractions to reconstitute in a cell-free system vesicle fusions occurring in the endocytic pathway. the endosomal fractions were prepared by immuno-isolation using as antigen an epitope located on a foreign protein, the transmembrane glycoprotein g (g-protein) of vesicular stomatitis virus. the g-protein was first implanted in the cell plasma membrane and subsequently endocytosed for 15 to 30 min at 37 degrees c. the endosomal fractions were immuno-isolated on a ...	1986	3028771
phospholipase c contracts visceral smooth muscle.	phospholipase c added into the muscle bath (0.2-1.5 units/ml) contracted the guinea-pig taenia coli in a concentration-dependent manner. the fully developed contraction appeared within second, like the contractile effect evoked by 5 microm acetylcholine. pharmacochemical dissection (using phospholipase d, indomethacin, neomycin, li+, phorbol ester and oleoyl-acetyl-glycerol) showed that the contractions induced by exogenous phospholipase c are mediated by inositol trisphosphate, not by diacylgly ...	1986	3028825
phospholipase c contracts visceral smooth muscle.	phospholipase c added into the muscle bath (0.2-1.5 units/ml) contracted the guinea-pig taenia coli in a concentration-dependent manner. the fully developed contraction appeared within second, like the contractile effect evoked by 5 microm acetylcholine. pharmacochemical dissection (using phospholipase d, indomethacin, neomycin, li+, phorbol ester and oleoyl-acetyl-glycerol) showed that the contractions induced by exogenous phospholipase c are mediated by inositol trisphosphate, not by diacylgly ...	1986	3028825
live dysentery vaccine "vadizen" (t32-istrati) immunogenicity against shigella-like challenge in animals.		1986	3300598
ultrastructural study on the bacterial symbiont in giardia lamblia.		1986	3300600
acute circulatory effects of doxorubicin in the conscious dog.	experimental work on the mechanisms of acute doxorubicin-induced cardiotoxicity may contribute to a better understanding of the clinical problem of cardiac failure after treatment with anthracycline derivatives. we studied aortic pressure and heart rate continuously for 1 h following a bolus injection of doxorubicin (1 mg/kg) in 7 dogs. in contrast with previous studies in intact animals, no anesthesia was used in order to eliminate possible interactions of doxorubicin with other drugs. one minu ...	1986	3153244
shiga toxin.		1986	3153582
humoral response in mice immunized with outer membrane proteins of shigella flexneri.	intraperitoneal immunization of mice with outer membrane proteins (omp) of sh. flexneri induced in the animals a synthesis of specific antibodies. their level determined by elisa test was found to be relatively low in the sera of animals immunized with a single dose (10 micrograms) of omp; it was markedly higher in mice immunized with two doses of omp, and very high after three fold immunization. the specific antibodies maintained in the animals for 8-16 weeks after immunization. anti-omp sera g ...	1986	3296993
the protein subunits of the double-stranded rna dependent rna polymerase and methyltransferase of the cytoplasmic polyhedrosis virus of silkworm, bombyx mori.	the particles of cpv of silkworm contain double-stranded rna polymerase and methyltransferase. it was reported in a previous paper that the genome-enzyme complex could be isolated. the genome-enzyme complex shows high enzyme activity of rna polymerase and methyltransferase in spite of the fact that it consists of only 5 percent of the protein. in order to clarify the protein subunits of the rna polymerase and methyltransferase, two methods were adopted. the sds-polyacrylamide gel electrophoretog ...	1986	3299695
plasmid-mediated contact haemolytic activity in shigella species: correlation with penetration into hela cells.	the main feature of virulent strains of shigella is their ability to invade eucaryotic cells. this phenotype is both plasmid-mediated and temperature-regulated. in the present report, we demonstrate a plasmid-mediated and temperature-regulated haemolytic activity in s. flexneri, s. dysenteriae and s. sonnei. detection of this haemolytic activity requires centrifugation of suspensions containing bacteria and erythrocytes, followed by incubation of the pellets at 37 degrees c. no soluble intra- or ...	1986	3322171
invasion of epithelial cells by shigellae.		1986	3322174
plasmid-mediated intracellular multiplication of shigella flexneri.		1986	3322175
natural cytotoxic effector cell activity against shigella flexneri-infected hela cells.	virus and facultative intracellular bacteria both replicate within a host cell. the recognition and killing of virus-infected cells by natural killer (nk) cells is thought to be an important host immune function. however, little is known about immune recognition of bacteria-infected cells. in this report, we show for the first time that human peripheral blood lymphocytes (pbl) and large granular lymphocytes (lgl) purified from pbl have significant levels of cytotoxic activity against shigella fl ...	1986	3510252
cutaneous shigellosis.		1986	3510587
shigellaemia in adults: case reports and literature review.		1986	3510864
rectal histology in acute bacillary dysentery.	a recent epidemic of acute shigella dysentery in west bengal (india) provided us with an opportunity to examine the rectal mucosal abnormalities seen in this condition. one hundred two patients were investigated using sigmoidoscopy, rectal biopsy, and rectal swab for culture. pure culture of shigella was obtained in 37 cases, and the rectal biopsy specimens from these patients were assessed in detail. the mean (+/- sd) duration of illness was 47.8 +/- 27.4 h (range 8-120 h), and most patients (3 ...	1986	3510937
multiplication of shigella flexneri within hela cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis.	virulent strains of shigella flexneri invade hela cells with high efficiency. this crucial step in the pathogenic process is encoded by a 140-megadalton plasmid which induces phagocytosis of the bacteria by host cells. in this report we used pwr100, the virulence plasmid of s. flexneri serotype 5, and phs4108, a 32-megadalton subclone of pwr100, to demonstrate that the plasmid is also responsible for rapid intracellular growth of the bacteria. the ability to replicate intracellularly was not cor ...	1986	3510976
molecular cloning of a genetic determinant for congo red binding ability which is essential for the virulence of shigella flexneri.	a dna sequence of about 1.0 kilobase (kb) derived from a 230-kb (140-megadalton) plasmid in a fully virulent shigella flexneri 2a strain, ysh6000, was cloned into escherichia coli k-12 by selecting for the ability to bind congo red (pcr+ phenotype). it was mapped and localized within the sali restriction fragment f on the plasmid. this clone converted an s. flexneri strain which is avirulent as a result of a small deletion in the plasmid to full virulence but did not convert those without the 23 ...	1986	3510977
expression of several adhesive macromolecules (n-cam, l1, j1, nile, uvomorulin, laminin, fibronectin, and a heparan sulfate proteoglycan) in embryonic, adult, and denervated adult skeletal muscle.	levels of the neural cell adhesion molecule n-cam in muscle are regulated in parallel with the susceptibility of muscle to innervation: n-cam is abundant on the surface of early embryonic myotubes, declines in level as development proceeds, reappears when adult muscles are denervated or paralyzed, and is lost after reinnervation (covault, j., and j. r. sanes, 1985, proc. natl. acad. sci. usa, 82:4544-4548). here we used immunocytochemical methods to compare this pattern of expression with those ...	1986	3511069
1-nitropyrene: a mutagenic product induced by the action of near ultraviolet light on 1-aminopyrene.	a solution of 1-aminopyrene in dimethyl sulfoxide exposed to an artificial source of near ultraviolet light (600 kj/m2) induced significant direct-acting mutagenicity in the ames/salmonella plating assay utilizing strain ta98. high-performance liquid chromatography of this solution resulted in a fraction that was mutagenic on ta98 but inactive on a nitroreductase-deficient strain of salmonella (ta98nr). this observation suggested the presence of a nitro-containing compound. mass spectral analysi ...	1986	3511370
adherence of non-fimbriate entero-invasive escherichia coli o124 to guinea pig intestinal tract in vitro and in vivo.	the adherence properties of non-fimbriate entero-invasive escherichia coli o124 in the guinea pig intestinal tract were studied. quantitative in-vitro determinations were done by incubating radiolabelled bacteria with suspensions of viable intestinal cells released by treating loops of the guinea pig intestine with solutions containing edta, dithiothreitol and citrate. non-bound bacteria were separated from the intestinal cells on a percoll gradient. only cells released from the colon, especiall ...	1986	3512837
activation of the complement system by cryptococcus neoformans leads to binding of ic3b to the yeast.	the complement system plays a key role in resistance to cryptococcosis. in the present study, we examined several factors that influence the binding of c3 cleavage fragments to cryptococcus neoformans. binding of c3 was determined by using normal human serum supplemented with 125i-labeled c3. incubation of encapsulated cryptococci in 20% serum led to the binding of approximately 3.2 x 10(6) molecules of c3 to each cell. the binding of c3 was markedly inhibited by heating the serum at 56 degrees ...	1986	3514450
degradation of insulin by insulin-degrading enzyme and biological characteristics of its fragments.	we have previously reported on the purification, characterization, and biological significance of insulin-degrading enzyme (ide) from pig and rat skeletal muscle. in the present study, we have investigated the detection and the hplc separation of degradation products of native insulin from the reaction of monocomponent porcine insulin with affinity-purified pig ide. insulin was degraded by ide in a time- and dose-dependent manner. eight peaks (peaks i through viii) appeared after 1 h of incubati ...	1986	3516656
coordinate roles of insulin and glucose on the growth of hepatoma cells in culture.		1986	3516664
the role of shigella spp., enteroinvasive escherichia coli, and other enteropathogens as causes of childhood dysentery in thailand.	shigella spp. were isolated from 44%, pleisomonas shigelloides from 22%, campylobacter spp. from 16%, and salmonella spp. from 10% of 200 thai children with mucoid or bloody diarrhea. enteroinvasive escherichia coli (eiec) was identified by examining isolates of e. coli for plasmids larger than 120 megadaltons (mda), by identifying e. coli with a virulence marker antigen in an elisa, and by performing dna hybridization with a 17-kilobase (kb) ecori digestion fragment of plasmid pwr100 (a 140-mda ...	1986	3517189
shigella bacteremia in adults.		1986	3517402
characterization of transferable plasmids from shigella flexneri 2a that confer resistance to trimethoprim, streptomycin, and sulfonamides.	a set of plasmids conferring resistance to several antibiotics, including the combination of trimethoprim and sulfamethoxazole, has been isolated from escherichia coli following conjugative cotransfer from a clinical isolate of shigella flexneri 2a. one of the plasmids, pcn1, was shown by subcloning and dna sequencing to carry a gene encoding a trimethoprim-insensitive dihydrofolate reductase identical to that found in e. coli transposon 7. this plasmid was also shown to confer resistance to bot ...	1986	3517903
[population migrations and the epidemic process of flexner and sonne dysentery].	analysis of the materials from the khabarovsk territory for the representative period of 25 years (1956-1980) revealed the influence of the migration of the population on the level and dynamics of morbidity in different kinds of dysentery, flexner's dysentery shown to occur more frequently than sonne dysentery. intensive migrations increase the proportion of susceptible persons among the population, thus facilitating the formation and circulation of shigella strains with pronounced virulence.	1986	3518299
[development of the classification of bacteria in the genus shigella].		1986	3518304
[bactericidal and immunocorrective properties of plant extracts].	extract from oak cork, st. john's wort leaves and flowers and pine buds possess more pronounced bactericidal properties with respect to staphylococci, shigellae, escherichia coli than decoctions from these medicinal plants. such extracts may be included into complexes for the treatment of otorhinolaryngological diseases, enterocolitis in children and bacterial eczema. as a medicinal form, extract prepared from medicinal plants are more convenient for storage and permit more exact dosage.	1986	3518308
comparative efficacies of single intravenous doses of ceftriaxone and ampicillin for shigellosis in a placebo-controlled trial.	to evaluate ceftriaxone for the treatment of shigellosis, 94 adult males with acute dysentery were randomly assigned to receive ceftriaxone (1 g), ampicillin (4 g), or saline placebo intravenously in single doses in a double-blind design. stool cultures were positive for shigella dysenteriae in 52 patients, s. flexneri in 38 patients, and other species in 4 patients. both ceftriaxone and ampicillin caused reductions in the mean duration of fever and the means of daily stool frequency 2 to 4 days ...	1986	3518625
metabolic rhythms in adolescents with diabetes during treatment with porcine or human insulin.	metabolic rhythms were studied over 24 hours in eight adolescent diabetic patients during treatment with porcine insulin and after transfer to human insulin. despite an increase in dose with human insulin no significant changes were found in fasting blood glucose, 24h mean blood glucose, or glycosylated haemoglobin concentrations. significantly higher 24h mean blood lactate concentrations and lower total ketone bodies and glycerol concentrations were observed during treatment with human insulin. ...	1986	3518640
identification and partial characterization of a nucleolar antigen with a molecular weight of 145,000 found in a broad range of human cancers.	previous studies in our laboratory have indicated the presence of nucleolar antigens in tumors which were not detected in normal tissues. some of the polyclonal antisera produced in these studies were shown to identify a mr, 145,000 nucleolar antigen on immunoblots of tumor nucleoli but not in normal human liver nucleoli. a monoclonal antibody to a mr 145,000 nucleolar protein (p145) was produced by immunization of mice with a nucleolar extract of hela cells which is enriched with this antigen. ...	1986	3518918
immunoglobulin g (igg) and iga subclass pattern of human antibodies to shigella flexneri and salmonella serogroup b and d lipopolysaccharide o antigens.	the subclass distribution of human serum antibodies to the o-antigenic lipopolysaccharides of salmonella serogroups b and d and to shigella flexneri serotypes 1b, 2a, and 4a lipopolysaccharide antigens were analyzed in an enzyme-linked immunosorbent assay with monoclonal antibodies to the immunoglobulin subclasses. the patients had culture-verified salmonella (17 swedes) or shigella flexneri (23 vietnamese; 11 children and 12 adults) infections. consecutive samples drawn during 1 year postinfect ...	1986	3519461
immunocytochemical localization of cathepsin b in rat kidney. ii. electron microscopic study using the protein a-gold technique.	thin sections of lowicryl k4m-embedded materials were labeled with protein a-gold complex. gold particles representing the antigen sites for cathepsin b were exclusively confined to lysosomes of each segment of the nephron. the heaviest labeling was noted in the lysosomes of the s1 segment of the proximal tubules. labeling intensity varied considerably with the individual lysosomes. lysosomes of the other tubular segments, such as the s2 and s3 segments of the proximal tubules, distal convoluted ...	1986	3519753
the relation between production of cytotoxin and clinical features in shigellosis.	the relation between in vitro production of hela cell cytotoxin by strains of shigella and clinical symptomatology was determined for 35 travelers from the united states who developed shigellosis in guadalajara, mexico. there were 25 patients with shigella sonnei, eight with shigella flexneri, one with shigella boydii, and one with shigella dysenteriae. these strains were evaluated for in vitro production of cytotoxin. the amount of cytotoxin did not correlate with the number of stools passed, t ...	1986	3519788
[dynamics of shigella interaction with the epithelium in the infection process].	the study of the adhesion of shigella flexneri to intestinal mucosal explants from human postabortion fetuses, used as an experimental model, has revealed that the process of interaction between the infective agent and the epithelium develops in accordance with langmuir's equation of the adsorption isotherm. the specific biological feature of the adhesive interaction between bacteria and the mucous membrane is the fact that the effective adhesion of microbes is possible only in case of their hig ...	1986	3521158
control of shigella flexneri in celebes black macaques (macaca nigra).	stool specimens collected systematically from a group of celebes black macaques (macaca nigra) with a high incidence of diarrhea were examined microbiologically. numerous isolates of shigella flexneri, campylobacter jejuni and pathogenic escherichia coli were recovered. previous parasitology reports had revealed that the majority of the animals had balantidium coli. subsequently, the group was treated with trimethoprim-sulfamethoxazole, erythromycin and tetracycline. after treatment, shigella fl ...	1986	3523037
[biochemical properties of shigella flexneri and their practical significance].	as the result of the study of 921 s. flexneri strains 1-6 and 4 (iv: 7,8), isolated in 31 regions of the ussr in 1975-1984, their biochemical characterization by 33 tests was made. all the strains under study proved to be typical in most of their constant signs, only some of strains 2a showed deviations in mannitol and some of strains 4a, in acetate. in strains of serovar 6, circulating in the ussr, specific features with respect to dulcitol and xylose were noted. the possibility of the biochemi ...	1986	3524079
[characteristics of the tissue population cycle of shigellae].	in experiments on human fetal intestinal explants infected with shigellae the specific multiplication rate of these infective agents, found to be 0.026, and the maximum level of their accumulation in erythrocytes, reaching 22-36 microbes per cell, have been determined. these phenomena can be observed after at least 3-hour incubation and end in the release of the infective agents from the affected area with shedding epithelial elements (villi). shigellae, aggregated in the shed villi easily adher ...	1986	3524080
[natural population migration and the dysentery epidemic process].	the complex analysis of materials obtained in different regions and territories of the far east makes it possible to establish that the natural migration of population affects dysentery morbidity by enhancing the susceptibility of human population to this infection. the internal mechanisms of the influence exerted on morbidity by the natural migration of population is disclosed from the viewpoint of the theory of the self regulation of the epidemic process.	1986	3524082
[multiple imaging procedures including mri in bladder cancer].	endoscopic photography, double contrast cystography, transurethral echography, x-ray ct scan, and mri (magnetic resonance imaging) were utilized for the staging diagnosis of the four patients with carcinoma of the bladder. in the first case, a 70-year-old man, since all of the five imaging procedures suggested a superficial and pedunculated tumor, his bladder cancer was considered t1. the classification of stage t3 carcinoma was made for the second 86-year-old male. because all of his imaging ex ...	1986	3524152
midbrain lesions, dopamine and male sexual behavior.	destruction of the medial preoptic area (mpoa) eliminates mating in male rats and this region is believed to play a major role in the control of male sexual behavior. efferents from the mpoa pass through and/or terminate in 4 midbrain regions: the dorsolateral tegmentum (dlt), the central gray, and the a9 and a10 areas. the present study reports the effects of bilateral destruction of each of these midbrain regions on brain catecholamines and sexual behavior in male rats. dlt lesions eliminated ...	1986	3524604
a genetic determinant required for continuous reinfection of adjacent cells on large plasmid in s. flexneri 2a.	we have identified a region (virg) on the 230 kb virulence plasmid of s. flexneri that is required for cell-to-cell spread of the bacterium. tn5 insertions into this region result in avirulent mutants that can initially invade and multiply in epithelial cells, but tend to lose active movement and tend to localize within the cytoplasm, where they are gradually extinguished without infecting adjacent cells. the virg region was localized to within 4 kb and may contain a single cistron. sequences hy ...	1986	3524856
crp genes of shigella flexneri, salmonella typhimurium, and escherichia coli.	the complete nucleotide sequences of the salmonella typhimurium lt2 and shigella flexneri 2b crp genes were determined and compared with those of the escherichia coli k-12 crp gene. the shigella flexneri gene was almost like the e. coli crp gene, with only four silent base pair changes. the s. typhimurium and e. coli crp genes presented a higher degree of divergence in their nucleotide sequence with 77 changes, but the corresponding amino acid sequences presented only one amino acid difference. ...	1986	3525518
hemodynamics of the legs and clinical symptoms following regional blocks for transurethral surgery.	in a prospective clinical study we compared the hemodynamics and clinical symptoms following regional blocks and general anesthesia. 115 patients undergoing transurethral resection of the prostate were randomized to spinal (n = 62) and epidural (n = 53) blocks. an additional 10 patients received general anesthesia. calf arterial flow, determined by strain gauge plethysmography (sgp), was similar pre- and postoperatively in the regional block groups but decreased in the general anesthesia group ( ...	1986	3527712
[effect of dichroa febrifuga l. on chloroquinsensible and chloroquinresistant malaria parasites].		1986	3528514
comparative histochemical distribution of nerve fibres storing noradrenaline and neuropeptide y (npy) in human ovary, fallopian tube, and uterus.	nerves containing noradrenaline were studied by formaldehyde-induced fluorescence and neuropeptide y (npy) was visualised by immunohistochemistry in the human ovary, fallopian tube and uterus. all structures were richly supplied with noradrenergic fibres closely associated with the vascular and non-vascular smooth musculature. npy-containing nerve terminals were consistently fewer, particularly in the ovary. the best developed nerve supply was found in the tubal isthmus and uterine cervix. vesse ...	1986	3528701
age-related changes in tissue metabolic rates and sensitivity to insulin in the chicken.	in vitro metabolic studies were conducted with tissues from broiler pullets at 4, 8, and 12 weeks of age. in order to accurately assess plasma insulin at each of these ages, blood samples were withdrawn via indwelling venous catheters at 30-min intervals for 6 hr, and plasma was assayed by a homologous chicken insulin (cins) radioimmunoassay. birds (n = 6) were sacrificed 24 hr following blood sampling and livers, hearts, and abdominal fat pads removed for metabolic studies. tissue slices of rem ...	1986	3529070
[effect of immunization with an enterobacterial vaccine on the rate of plating out of shigella from the intestinal mucosa of mice orally infected].	the oral and subcutaneous immunization of mice with the vaccine prepared from s. minnesota strain r595, chemotype re, known as enterobacterial vaccine, was found to significantly decrease the number of shigellae in cultures obtained by the inoculation of homogenized mucosal samples taken from the large intestine of mice, the vaccine prepared from s. minnesota isogeneous strain sf 1111 with the intact structure of lipopolysaccharide had no such activity. antisera, obtained by the immunization of ...	1986	3529760
[intrahepatic cholestasis due to shigella in an infant].		1986	3530915
comparison of dna probes and the sereny test for identification of invasive shigella and escherichia coli strains.	forty-two shigella and 29 escherichia coli strains were screened for invasiveness in the sereny test and for hybridization with two recently described dna probes for the invasiveness plasmid. both probes produced identical results. all sereny-positive strains hybridized with both dna probes. three sereny-negative strains also hybridized with the probes, suggesting that there are strains containing the invasiveness plasmid that are not pathogenic in animal models.	1986	3531233
characterization of shigella flexneri-specific murine monoclonal antibodies by chemically defined glycoconjugates.	chemically defined glycoconjugates are demonstrated to have considerable potential for selecting hybridoma antibodies directed toward o-antigenic determinants, especially when used in combination with a panel of well-characterized lps molecules. monoclonal antibodies specific for the shigella flexneri o-antigens of serogroup 5b, variants x and y, were generated after immunization of balb/c mice with killed bacterial cells, and active hybrids were selected on the basis of elisa performed with the ...	1986	3531329
[phagocytizing blood cells in dysentery].		1986	3532361
[detection of immunosuppression factors in shigella sonnei].	a factor, making noninvasive shigellae and other bacteria capable of suppressing immunological memory and secondary immune response manifested as delayed hypersensitivity, has been detected in the germ-free filtrates of the broth cultures of invasive s. sonnei.	1986	3532643
[general and local reactions in the proteolytic system during the development of experimental pneumonia].	proteolytic enzymes and their inhibitors were studied in the serum and bronchoalveolar lavage of rats with acute and chronic experimental pulmonary inflammation. an increase in the inhibitory potential of the blood serum and bronchoalveolar lavage at the acute stage of inflammation was shown. at the chronic stage of pulmonary inflammation the level of the main inhibitors decreased to normal or zero values in the blood and lavage fluid, respectively. it was observed that alpha 1-proteinase inhibi ...	1986	3533173
epidemic reiter's syndrome following an outbreak of shigellosis.	we prospectively studied the occurrence of reiter's syndrome (rs) or reactive arthritis (rea) in 205 of 349 cruise-ship passengers who attended a buffet ashore and developed shigella flexneri 2a enteritis. five passengers probably had rs/rea and 16 were possible or doubtful cases of rs/rea. hla-b27 was identified in 4 of 5 probable rs cases, but was not present in any of the 16 possible or doubtful cases nor in any of 20 passengers (controls) without any symptoms of rs/rea. there was no statisti ...	1986	3533609
lack of serologically defined arthritogenic shigella flexneri cell envelope antigens in post-dysenteric arthritis.	post-dysenteric or reactive arthritis (rea) is closely associated with hla-b27. this histocompatibility antigen is heterogeneous and consists of 2 serologically defined variants: b27m1+m2+ and b27m1+m2-. this paper gives a qualitative evaluation of the antibodies present in the sera of 62 patients with dysentery due to shigella flexneri 2a, a known arthritogenic bacterium. the patients were classified in 4 groups: b27m1+m2+rea+ (n = 5), b27m1+m2+rea- (n = 7); b27m1+m2-rea- (n = 1); b27-rea- (n = ...	1986	3533766
enzyme immunoassay for detection of salmonella in foods: collaborative study.	a collaborative study was performed in 25 laboratories to validate an enzyme immunoassay (eia) procedure utilizing 2 specific monoclonal antibodies for rapid detection of salmonella in foods. the eia was compared with the standard culture procedure for detection of salmonella in 6 food types: ground black pepper, soy isolate, dried whole eggs, milk chocolate, nonfat dry milk, and raw deboned turkey. uninoculated and inoculated samples were included in each food group analyzed, with the exception ...	1986	3533896
[evaluation of the antibiotic sensitivity of pseudomonas sp. using the api-atb-pse system. comparison with diffusion and the reference agar dilution method].	in vitro activity of twelve antibiotics (ticarcillin, mezlocillin, azlocillin, piperacillin, cefoperazone, cefsulodin, ceftazidime, gentamicin, netilmicin, pefloxacin and ciprofloxacin) was determined by measuring minimum inhibitory concentrations (mics) using agar dilution according to who recommendations, agar diffusion and the api-atb-pse system. one-hundred and forty-two pseudomonas strains were studied. five species of pseudomonas were represented, i.e. aeruginosa, maltophilia, cepacia, stu ...	1986	3534715
serogroup frequency and drug sensitivity of shigella strains encountered at the university hospital of the west indies.		1986	3535257
shigella dysenteriae type 1 enterocolitis.	shigella dysenteriae type 1 is much more virulent than shigella flexneri and sonnei which are endemic in australia. this report describes a 22 year old woman who acquired shigella dysenteriae type 1 whilst travelling in india. during the course of her illness, she developed severe enterocolitis for which a subtotal colectomy was performed. the illness resembled fulminant ulcerative colitis and its infectious nature was difficult to establish because several fecal cultures failed to grow the path ...	1986	3535768
interleukin-1 (22-k factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes.	an electrophoretically pure preparation of natural human interleukin-1 (il-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. day 4 myeloid cluster-forming cells (cfc), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (cfu-gm) were stimulated in a dose-dependent fashion. at optimal concentrations of il-1, the number of day 4 cfc reached 72%, the number of day 7 cfu-gm reached 32%, and the number of day 10 cfu-gm reached 80% of ...	1986	3535928
studies on the mechanics of the normal human middle ear.	the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ...	1986	3536194
widal-type serology using live antigen for diagnosis of shigella flexneri dysentery.		1986	3536499
salmonella/microsome mutagenicity tests of heat-processed milk samples.	samples of whole milk were heat treated by commercial heat-sterilization, by commercial heat-pasteurization or by a laboratory heat-pasteurization (65 degrees c for 30 min). each sample was tested for mutagenicity in salmonella typhimurium strains ta98 and ta100 with and without s-9 mix. dichloromethane extracts of milk heated at 100, 135 and 150 degrees c for 5 hr were also tested for mutagenicity using the same assay. none of these samples exhibited mutagenicity in the ames assay used.	1986	3536696
secretory immunoglobulin a response following peroral priming and challenge with shigella flexneri lacking the 140-megadalton virulence plasmid.	this study evaluates the ability of noninvasive shigella spp., lacking the 140-megadalton virulence plasmid, to elicit a mucosal immunoglobulin a immune response in the intestine. for these studies, we used shigella flexneri m4243a1 (which lacks the plasmid and is sereny test negative) to prime and challenge three groups of rabbits perorally. both primary and immunoglobulin a memory responses were detectable in these secretions. these findings indicate that a mucosal memory response can be prime ...	1986	3536753
a solid-phase enzyme immunoassay for the determination of igm and igg antibodies against translation products of pre-s1 and pre-s2 regions of hepatitis b virus.	the envelope of hepatitis b virus is coded for by pre-s1, pre-s2 regions and the s gene. a method was developed to determine antibody to the product of pre-s1 region (anti-pre-s1) and antibody to the product of pre-s2 region (anti-pre-s2), either of igm or igg class, by a solid-phase enzyme immunoassay. for the determination of anti-pre-s1, tubular particles containing translation products of pre-s1, pre-s2 regions and the s gene were broken into constituent envelope polypeptides and immobilized ...	1986	3537138
a solid-phase enzyme immunoassay for the determination of igm and igg antibodies against translation products of pre-s1 and pre-s2 regions of hepatitis b virus.	the envelope of hepatitis b virus is coded for by pre-s1, pre-s2 regions and the s gene. a method was developed to determine antibody to the product of pre-s1 region (anti-pre-s1) and antibody to the product of pre-s2 region (anti-pre-s2), either of igm or igg class, by a solid-phase enzyme immunoassay. for the determination of anti-pre-s1, tubular particles containing translation products of pre-s1, pre-s2 regions and the s gene were broken into constituent envelope polypeptides and immobilized ...	1986	3537138
production of shiga toxin and other cytotoxins by serogroups of shigella.	we measured the cytotoxic activity of 119 strains of shigella by using a quantitative [3h]thymidine-labeled hela cell assay. we assayed 13 strains of shigella dysenteriae 1; 18 strains of s. dysenteriae types 2 and 3; and 88 strains of shigella sonnei, shigella flexneri, and shigella boydii. strains of s. dysenteriae 1 demonstrated high levels of cytotoxicity (geometric mean, 10(5.04) cd50/mg of protein; range, 10(3.95)-10(6.10). cytotoxic activities of the non-type 1 strains of s. dysenteriae a ...	1986	3537154
surface hydrophobicity of plasmid-carrying virulent shigella flexneri and their avirulent variants.	five pairs of strains of s. flexneri each differing in the colour of their colonies after growth on congo red agar have been tested for their ability to cause keratoconjunctivitis in the guinea pig eye, for the presence of the 140 md virulence plasmid, for the presence of the virulence marker antigen, and for their ability to adsorb to hydrophobic surfaces (cellulose nitrate filters and phenyl sepharose). the results suggest that the presence of the 140 md virulence plasmid provides the bacteria ...	1986	3537274
modelling of dna sedimentation profiles from irradiated eukaryotic cells.	a simple mathematical model of dsb distributions in dna from irradiated interphase eukaryotic cells is considered and an approximate description of resulting dna sedimentation profiles is derived. the model takes into account the differences found between the radiosensitivities of heterochromatin and euchromatin and is based, contrary to the usual approach, on a non-homogeneous distribution of dsb in eukaryotic dna. comparison with some experimental dna sedimentation profiles prefers the propose ...	1986	3537531
phenotypic characterization of the thymus microenvironment study of the human thymus architecture.	phenotypic characterization of the human thymic microenvironment shows the heterogeneity of the epithelial component. using monoclonal antibody l191, we define here a new antigen common to the flat epithelial cells lining up the thymic capsule and septum, stellate epithelial cells from the subcapsular area and the medulla (m), but lacking on stellate epithelial cells from the inner cortex. thus, this cell population can be characterized by a series of antigens (te-4 and p19--shared with htlv--pr ...	1986	3541301
[ultrastructural study of intestinal bacteria grown on endo- and levine-type nutrient media based on nonfood raw materials].	the comparative electron-microscopic study of several test strains (salmonella typhi h-901, shigella flexneri la 8516, and escherichia coli 055) grown in experimental endo and levine media prepared on the basis of raw materials unsuitable for human consumption and in commonly used similar media prepared on the basis of sprat hydrolysate has shown the test strains grown in media containing aminopeptide and fodder yeast hydrolysate to retain their typical ultrastructure, which confirms the possibi ...	1986	3541464
[characteristics of erythrocyte diagnostic agents studied using optical methods].	the quantitative characterization of erythrocyte diagnosticums (ed) has been made by optical methods (light microscopy with the use of an image analyzer, model magiscan 2, and the opacity spectrum technique). the following parameters of ed have been determined: the average of the major axis (5.25 +/- 0.57 micron for ed from shigella sonnei and 5.53 +/- 0.50 micron for ed from shigella flexneri), the ratio of semiaxes (p approximately equal to 3), the major axis length distribution, the refractiv ...	1986	3541465
[chromosomal changes in patients with acute dysentery and their relation to immunologic shifts].	the correlation between the dynamics of the occurrence of the class of lymphocytes without chromosomal associations and with two associating acrocentric chromosomes (cl0 + 2) and the course of the disease, as well as the development of immunity in patients with acute dysentery, has been detected. the positive correlation between the occurrence of cl0 + 2 and cell-mediated immunity reactions presupposes the possibility of using this cytogenetic characteristic for evaluating the cell-mediated immu ...	1986	3541467
[bacteriological study of shigellosis in the lake kivu area (central africa). developments in the last 15 years (1968-1983)].	1913 shigella strains were isolated from 1968 to 1983 in lake kivu area (central africa). distribution and evolution of serotypes, biotypes and antibiotypes were studied. the majority of serotypes and biotypes were stable. however, few of these strains showed variations, among them shigella dysenteriae serotype 1, causing epidemic dysenteriae since 1981. epidemiologic survey of shigellosis in kivu seems to be rather underdeveloped (4 bacteriology laboratories for an area of 10 million inhabitant ...	1986	3542256
canine lymphoma: clinical considerations.		1986	3333887
canine lymphoma: clinical considerations.		1986	3333887
study of ileostomy in dogs, functional aspects, optic and scanning electronic microscopy.	containing ileostomy has been a permanent aim of researchers. different technics for containing in the ileal derivations has been proposed, very little filled the requisites, but no one reached the desired aim, and the problem continues unsolved. the authors, based in experimental study in two groups of dogs submitted to ileostomy, with and without preservation of ileal pylorus, observed the following parameters: weight dog clinical evaluation of animals (diarrhea, infection of surgical wound an ...	1986	3435265
osteogenic sarcoma and soft tissue myxoma in a patient with fibrous dysplasia and hemoglobins jbaltimore and s.	a 41-year-old man with recognized polyostotic fibrous dysplasia since late childhood developed fibroblastic osteogenic sarcoma in the left tibia. four months after the initial diagnosis, an intramuscular myxoma was discovered in the left thigh. twenty years previously he had been found to be heterozygous for hemoglobins jbaltimore and s. malignant transformation in fibrous dysplasia is unusual and may be associated in some individuals with prior irradiation. soft tissue myxomas associated with f ...	1986	3456858
alpha-transforming growth factor secreted by untransformed bovine anterior pituitary cells in culture. ii. identification using a sequence-specific monoclonal antibody.	untransformed bovine anterior pituitary cells cultured in serum-free defined medium secrete an epidermal growth factor (egf)-like peptide with an amino acid composition similar to rat or human alpha-transforming growth factor (alpha tgf). to further characterize the bovine pituitary alpha tgf, it was compared to a human alpha tgf partially purified from the conditioned medium of a human melanoma cell line. an anti-alpha tgf monoclonal antibody, mf9, was produced from hybridomas derived from mice ...	1986	3464598
[possibility of overcoming of acnu resistance in an acnu-resistant subline of c6 rat glioma].	membrane-modifying agents such as reserpine, calcium antagonists (nicardipine, verapamil) and calmodulin inhibitor (trifluoperazine) were found to enhance the cytotoxicity of acnu in vitro and in vivo in acnu-resistant c 6 (c 6/acnu) glioma. in in vitro experiments, uptake and retention studies with [14c] acnu revealed that intracellular uptake and retention of acnu in c 6 cells were larger than those in c 6/acnu cells, and that these membrane-modifying agents increased the cellular uptake and r ...	1986	3468959
local treatment with epurox (superoxide dismutase and catalase of human origin) in the chronic inflammatory and degenerative rheumatism.		1986	3496868
[evaluation of vaccinal immunogenesis by the peroxidase method].	the possibility of evaluating functional immunomorphogenesis in the course of the vaccinal process after the injection of conjugated and live brucellosis vaccines, as well as conjugated plague antigen and yersinia pestis strain ev, to guinea pigs has been shown by means of the direct and two-layer variants of the immunoperoxidase assay. the dynamics of the accumulation of globulin-producing cells in the immunocompetent organs and the time course of immunoglobulin titers in the peripheral blood a ...	1986	3548171
biochemical and functional characterization of the rat liver glucose-transport system. comparisons with the adipocyte glucose-transport system.	the properties of the glucose-transport systems in rat adipocytes and hepatocytes were compared in cells prepared from the same animals. hormones and other agents which cause a large stimulation of 3-o-methylglucose transport in adipocytes were without acute effect in hepatocytes. hepatocytes displayed a lower affinity for 3-o-methylglucose (20 mm) and alternative substrates than adipocytes (6 mm), whereas inhibitor affinities were similar in both cell types. the concentration and distribution o ...	1986	3548706
effects of bacterial lipopolysaccharide on the binding of lymphocytes to endothelial cell monolayers.	preincubation of human umbilical vein endothelial cell (ec) monolayers with 1 ng to 10 micrograms/ml lipopolysaccharide (lps) increased the binding of t lymphocytes to ec. the effect was maximal at lps concentrations of 0.1 to 10 micrograms/ml, and occurred with lps derived from escherichia coli (serotypes 0111:b4 and 0127:b8), shigella flexneri (serotype 2a), serratia marcescens (serotype 0:3), and yersinia entercolitica (serotype 0:3). the increased binding appeared to be mediated primarily th ...	1986	3484495
improved survival after allogeneic small intestinal transplantation in the rat using cyclosporine immunosuppression.	we investigated the effect of cyclosporine on survival after intestinal transplantation between histoincompatible brown norway (agb3/3) and lewis (agb1/1) rats. intestinal grafts were primarily vascularized (by microsurgical techniques) and interposed isoperistaltically in the recipient's jejunum. all animals were weighed and observed daily. survival of recipients given cyclosporine 20 mg/kg/d (112 +/- 92 days, n = 10) was significantly longer (p less than .02) than that of recipients given no d ...	1986	3485711
characterisation of haemophilus spp. isolated from infant conjunctivitis.	haemophilus strains isolated from children under the age of 11 months with conjunctivitis were characterised by biotype, sugar fermentation, plasmid pattern and outer-membrane-protein profiles. h. influenzae was the most common species identified and was separated into 14 groups based on sugar fermentation and biotype patterns and into more than 20 groups when plasmid and outer-membrane-protein profiles were included. small (mol. wt less than 10 x 10(6)) plasmids were identified in 11 of 34 (32% ...	1986	3486294
possible role of protein phosphorylation in the mitogenic effect of high density lipoproteins on cultured vascular endothelial cells.	the implication of protein phosphorylation in the mitogenic action of high density lipoproteins (hdl) on bovine vascular endothelial cells was investigated by incubating endothelial cell cultures in the presence of 32p-labeled phosphoric acid. the incorporation of 32p into proteins was measured after fractionation by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and autoradiography of the gel. in endothelial cells seeded at low density and made quiescent by serum s ...	1986	3486870
acrocentric/18p translocation in two mentally retarded males. delineation of the adult phenotype.	in this report we describe two adult male patients with a chromosomal rearrangement involving the short arm of chromosome 18 and an acrocentric chromosome. in addition to moderate mental retardation and verbal disability they presented dysmorphic stigmata similar to those found in the noonan syndrome.	1986	3490204
the use of rodent models in assessing antimicrobial activity against mycobacterium leprae.		1986	3553807
limited in vitro multiplication of mycobacterium leprae: application to screening potential antileprosy compounds.		1986	3553808
optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain.	samples of rat and human cerebral cortex were frozen, stored, and thawed under a variety of conditions to define further the optimal procedure for storing human brain samples for subsequent metabolic and functional studies that use incubated synaptosomes. tissue samples were best preserved by immersing them in isotonic sucrose prior to slow freezing, but there was no advantage in first chopping up the material. high concentrations of sucrose, rather than exerting a cryoprotective effect, were de ...	1986	3561893
[structural studies of panax ginseng c. a. mey pectin].		1986	3591331
determination of mevinolin and mevinolinic acid in plasma and bile by reversed-phase high-performance liquid chromatography.		1986	3644824