Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| transgenic alteration of toll immune pathway in the female mosquito aedes aegypti. | reverse genetics is a powerful tool for understanding gene functions and their interactions in the mosquito innate immunity. we took the transgenic approach, in combination with the rna interference (rnai) technique, to elucidate the role of mosquito rel1, a homolog of drosophila dorsal, in regulation of toll immune pathway in the mosquito aedes aegypti. by transforming the mosquitoes with deltarel1-a or a double-stranded rna construct of rel1 driven by the female fat body-specific vitellogenin ... | 2005 | 16157887 |
| characterization of enterobacteria using maldi-tof mass spectrometry. | a method is proposed for the rapid classification of gram-negative enterobacteria using on-slide solubilization and trypsin digestion of proteins, followed by maldi-tof ms analysis. peptides were identified from tryptic digests using microsequencing by tandem mass spectrometry and database searches. proteins from the outer membrane family (omp) were consistently identified in the enterobacteria escherichia coli, enterobacter cloacae, erwinia herbicola, and salmonella typhimurium. database search ... | 2005 | 16159146 |
| nosocomial urosepsis caused by enterobacter kobei with aberrant phenotype. | enterobacter kobei is the species of the enterobacter cloacae complex, which is phenotypically most closely related to the species e. cloacae. this is the first report of infection caused by a new biotype of e. kobei. a patient with a history of urinary bladder operation developed a urosepsis with an enterobacter isolate displaying the e. cloacae phenotype. the isolate was classified to the species e. kobei by sequence analysis of the 16s-rdna, 4 protein-coding genes and enterobacterial repetiti ... | 2005 | 16168611 |
| eustachian valve endocarditis: a rare localization of right side endocarditis. a case report and review of the literature. | right-sided endocarditis occurs predominantly in intravenous drug users, in patients with pacemaker or central venous lines and in patients with congenital heart disease. the vast majority of cases involve the tricuspid valve. eustachian valve endocarditis is an uncommon disease with similar signs and symptoms of the tricuspid valve endocarditis. a series of only 16 cases of eustachian valve endocarditis are reported in the literature. | 2005 | 16171516 |
| specificity of enterobacterial repetitive intergenic consensus and repetitive extragenic palindromic polymerase chain reaction for the detection of clonality within the enterobacter cloacae complex. | an increasing number of clonal outbreaks caused by members of the e. cloacae complex is being reported. for the detection of clonality, pulsed-field gel electrophoresis (pfge) is considered the golden standard, but pcr-based methods are cheaper, easier to perform, and provide faster results. one hundred ninety-five isolates of the e. cloacae complex isolated at the university hospital grosshadern, munich, germany, were assigned to their respective genetic cluster by partial hsp60 sequencing. all ... | 2005 | 16182074 |
| in vitro and in vivo antibacterial activities of sm-216601, a new broad-spectrum parenteral carbapenem. | sm-216601 is a novel parenteral 1beta-methylcarbapenem. in agar dilution susceptibility testing, the mic of sm-216601 for 90% of the methicillin-resistant staphylococcus aureus (mrsa) strains tested (mic(90)) was 2 microg/ml, which was comparable to those of vancomycin and linezolid. sm-216601 was also very potent against enterococcus faecium, including vancomycin-resistant strains (mic(90) = 8 microg/ml). sm-216601 exhibited potent activity against penicillin-resistant streptococcus pneumoniae, ... | 2005 | 16189097 |
| genetic and enzymatic properties of metallo-beta-lactamase vim-5 from a clinical isolate of enterobacter cloacae. | a vim-5-producing enterobacter cloacae isolate (edv/1) was identified in a collection of clinical strains stored before 2002. the gene, bla(vim-5), was located on a 2,712-bp bamhi-hindiii fragment of a 23-kbp (approximately) nonconjugative plasmid (pedv5) in a class 1 integron as a single gene cassette. | 2005 | 16189133 |
| [pathogenic characters of infected bacteria after liver transplantation]. | to analyze the main pathogens of infection after the liver transplantation and their antibiotic resistant patterns. | 2005 | 16190391 |
| increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in gaza strip, palestine. | this article presents the incidence of ciprofloxacin resistance among 480 clinical isolates obtained from patients with urinary tract infection (uti) during january to june 2004 in gaza strip, palestine. the resistance rates observed were 15.0% to ciprofloxacin, 82.5% to amoxycillin, 64.4% to cotrimoxazole, 63.1% to doxycycline, 32.5% to cephalexin, 31.9% to nalidixic acid, and 10.0% to amikacin. high resistance to ciprofloxacin was detected among acinetobacter haemolyticus (28.6%), staphylococc ... | 2005 | 16192681 |
| spread of novel expanded-spectrum beta-lactamases in enterobacteriaceae in a university hospital in the paris area, france. | in 2002, 28 non-duplicate enterobacterial isolates producing extended-spectrum beta-lactamases (esbls) were collected from infected patients at the bicêtre hospital in paris, france. escherichia coli was the predominant esbl-positive enterobacterial species, comprising ten (36%) of the isolates. ctx-m enzymes (ctx-m-3, ctx-m-10, ctx-m-14 and ctx-m-15) were produced by 11 (39%) of the isolates (six e. coli, two enterobacter cloacae, one enterobacter aerogenes, one proteus mirabilis and one citrob ... | 2005 | 15966981 |
| two new sinorhizobium meliloti lysr-type transcriptional regulators required for nodulation. | the establishment of an effective nitrogen-fixing symbiosis between sinorhizobium meliloti and its legume host alfalfa (medicago sativa) depends on the timely expression of nodulation genes that are controlled by lysr-type regulators. ninety putative genes coding for lysr-type transcriptional regulators were identified in the recently sequenced s. meliloti genome. all 90 putative lysr genes were mutagenized using plasmid insertions as a first step toward determining their roles in symbiosis. two ... | 2005 | 15968067 |
| an enterobacter cloacae toxin able to generate oxidative stress and to provoke dose-dependent lysis of leukocytes. | we investigated an enterobacter cloacae strain exhibiting high hemolytic and leukotoxic activity. monomeric and polymeric forms of the toxin showed similar effects on blood cells, although the polymer was more active than the monomer. fluorescence microscopy revealed that both forms of the fitc-labeled toxin interacted with leukocytes, principally with neutrophils. prelytic concentrations of polymeric and monomeric toxin significantly increased the production of reactive oxygen species (ros) in ... | 2005 | 15969471 |
| isolation and characterization of enteric bacteria from the hindgut of formosan termite. | the formosan subterranean termite, coptotermes formosanus shiraki, is an aggressive, invasive termite species that has caused billions of dollars of damage across the united states for the past 50 years. termites depend on intestinal microorganisms for cellulose digestion. symbiotic microorganisms in the termite gut play key physiological functions such as cellulose and hemicellulose digestion, acetogenesis, hydrogenesis, methanogenesis, sulfate reduction, and nitrogen fixation. additionally, in ... | 2005 | 15978992 |
| distribution of genes encoding the microbial non-oxidative reversible hydroxyarylic acid decarboxylases/phenol carboxylases. | bacterial non-oxidative, reversible multi subunit hydroxyarylic acid decarboxylases/phenol carboxylases are encoded by the three clustered genes, b, c, and d, of approximately 0.6, 1.4, and 0.2 kb, respectively. there are more than 160 homologues in the database with significant similarity to gene b (homology to ubix) and c (ubid) distributed in all three microbial domains, however, homologues to gene d, are not numerous ( approximately 15). the occurrence of the entire bcd gene cluster encoding ... | 2005 | 15979273 |
| antimicrobial activity of novel dendrimeric peptides obtained by phage display selection and rational modification. | a large 10-mer phage peptide library was panned against whole escherichia coli cells, and an antimicrobial peptide (qekirvrlsa) was selected. the peptide was synthesized in monomeric and dendrimeric tetrabranched form (multiple antigen peptide [map]), which generally allows a dramatic increase of peptide stability to peptidases and proteases. the antibacterial activity of the dendrimeric peptide against e. coli was much higher than that of the monomeric form. modification of the original sequenc ... | 2005 | 15980334 |
| worldwide disseminated arma aminoglycoside resistance methylase gene is borne by composite transposon tn1548. | the arma (aminoglycoside resistance methylase) gene, which confers resistance to 4,6-disubstituted deoxystreptamines and fortimicin, was initially found in klebsiella pneumoniae bm4536 on incl/m plasmid pip1204 of ca. 90 kb which also encodes the extended-spectrum beta-lactamase ctx-m-3. thirty-four enterobacteria from various countries that were likely to produce a ctx-m enzyme since they were more resistant to cefotaxime than to ceftazidime were studied. the arma gene was detected in 12 clinic ... | 2005 | 15980373 |
| in vitro synergy of ciprofloxacin and gatifloxacin against ciprofloxacin-resistant pseudomonas aeruginosa. | multidrug-resistant pseudomonas aeruginosa with combined decreased susceptibility to ceftazidime, ciprofloxacin, imipenem, and piperacillin is increasingly being found as a cause of nosocomial infections. it is important to look for combinations of drugs that might be synergistic. ciprofloxacin resistance by p. aeruginosa is mediated in part by an efflux pump mechanism. gatifloxacin, an 8-methoxyfluoroquinolone, inhibits a staphylococcal efflux pump. an earlier in vitro study using an etest syne ... | 2005 | 15980375 |
| broader distribution of plasmid-mediated quinolone resistance in the united states. | the plasmid-encoded quinolone resistance gene qnra confers low-level quinolone resistance, facilitating selection of higher-level resistance. epidemiologic surveys for qnra were extended to isolates of enterobacter spp. and to quinolone-susceptible enterobacteriaceae. two (10%) of 20 ceftazidime-resistant quinolone-susceptible klebsiella pneumoniae strains carried the gene, as did 12 (17%) of 71 ceftazidime-resistant enterobacter strains from across the united states. one of these enterobacter i ... | 2005 | 15980384 |
| association of plasmid-mediated quinolone resistance with extended-spectrum beta-lactamase veb-1. | association of the plasmid-mediated quinolone resistance determinant qnra and the bla(veb-1) gene was identified in a single enterobacter cloacae isolate from k.-bicêtre, france, and in 11 out of 23 bla(veb-1)-positive enterobacterial isolates from bangkok, thailand. this result may explain in part the association between quinolone and extended-spectrum beta-lactam resistance. | 2005 | 15980408 |
| gram-negative bacilli associated with catheter-associated and non-catheter-associated bloodstream infections and hand carriage by healthcare workers in neonatal intensive care units. | bloodstream infections caused by gram-negative bacilli are a substantial cause of morbidity and mortality in infants in neonatal intensive care units. this study describes the species of gram-negative bacilli causing bloodstream infections in two neonatal intensive care units, compares characteristics of catheter-related and non-catheter-related bloodstream infections, and compares species and antibiotic resistance patterns of these organisms with those isolated from the hands of nurses working ... | 2005 | 15982435 |
| in-house validation of the bactec 9240 blood culture system for detection of bacterial contamination in platelet concentrates. | at present, only two commercially available automated culture systems are cleared by the fda for the purpose of quality control (qc) testing for bacterial contamination of platelet (plt) concentrates: the bact/alert blood culture system (biomérieux) and the pall ebds (pall corporation), both of which allow testing of leukoreduced apheresis as well as whole blood-derived plts. after the decision of the aabb to institute universal qc testing of plt concentrates for evidence of bacterial contaminat ... | 2005 | 15987359 |
| adhesion of the probiotic bacterium lactobacillus plantarum 299v onto the gut mucosa in critically ill patients: a randomised open trial. | to achieve any possible positive effect on the intestinal mucosa cells it is important that probiotics adhere tightly onto the intestinal mucosa. it has been shown in healthy volunteers that lactobacillus plantarum 299v (lp 299v) (dsm 9843), a probiotic bacterium, given orally in a fermented oatmeal formula adheres onto the intestinal mucosa, but whether this also occurs in critically ill patients is unknown. | 2005 | 15987403 |
| assembly of cyclic enterobacterial common antigen in escherichia coli k-12. | we describe here the purification and quantification of a water-soluble cyclic form of enterobacterial common antigen (eca(cyc)) from escherichia coli k-12 as well as information regarding its subcellular location and the genetic loci involved in its assembly. structural characterization of purified eca(cyc) molecules obtained from e. coli k-12 revealed that they uniformly contained four trisaccharide repeat units, and they were substituted with from zero to four o-acetyl groups. cells from over ... | 2005 | 16199561 |
| specificity and reversibility of the transpeptidation reaction catalyzed by the streptomyces r61 d-ala-d-ala peptidase. | the specificity of the streptomyces r61 penicillin-sensitive d-ala-d-ala peptidase has been re-examined with the help of synthetic substrates. the products of the transpeptidation reactions obtained with gly-l-xaa dipeptides as acceptor substrates are themselves poor substrates of the enzyme. this is in apparent contradiction with the classically accepted specificity rules for d-ala-d-ala peptidases. the gly-l-xaa dipeptide is regenerated by both the hydrolysis and transpeptidation reactions. th ... | 2005 | 16199665 |
| helicase89b is a mot1p/btaf1 homologue that mediates an antimicrobial response in drosophila. | we have identified a novel component, helicase89b, that is required for the inducible antimicrobial response in drosophila larvae by means of a p-element insertional genetic screen. helicase89b belongs to the mot1p/btaf1 subfamily of snf2-like atpases. this subfamily can interact with tata-binding proteins, but whether the interaction leads to gene activation or repression is being debated. we found that helicase89b is required for the inducible expression of antimicrobial peptide genes but not ... | 2005 | 16200050 |
| hfq-dependent regulation of ompa synthesis is mediated by an antisense rna. | this paper shows that the small rna mica (previously srad) is an antisense regulator of ompa in escherichia coli. mica accumulates upon entry into stationary phase and down-regulates the level of ompa mrna. regulation of ompa (outer membrane protein a), previously attributed to hfq/mrna binding, is lost upon deletion of the mica gene, whereas overexpression of mica inhibits the synthesis of ompa. in vitro, mica binds to the ompa mrna leader. enzymatic and chemical probing was used to map the str ... | 2005 | 16204185 |
| shv-type extended-spectrum beta-lactamase production is associated with reduced cefepime susceptibility in enterobacter cloacae. | cefepime is a potentially useful antibiotic for treatment of infections with enterobacter cloacae. however, in our institution the mic(90) for e. cloacae bloodstream isolates is 16 microg/ml. pcr amplification of bla genes revealed that one-third (15/45) of e. cloacae bloodstream isolates produced shv-type extended-spectrum beta-lactamases (esbls) in addition to hyperproduction of ampc-type beta-lactamases. the majority (11/15) of esbl producers also produced the tem-1 beta-lactamase. the shv ty ... | 2005 | 16207962 |
| molecular characterization of hand flora and environmental isolates in a community setting. | we analyzed 69 bacterial isolates, comprising seven species of gram-negative bacterial rods and three species of coagulase-negative staphylococci, recovered from both the hands of caretakers and their environment in households sampled in upper manhattan. repetitive sequence-based pcr and dendrogram analysis were used to determine strain similarity. greater than 25% of individual species of acinetobacter, enterobacter, and coagulase-negative staphylococci recovered from the hands and immediate en ... | 2005 | 16207984 |
| contribution of systematic serological testing in diagnosis of infective endocarditis. | despite progress with diagnostic criteria, the type and timing of laboratory tests used to diagnose infective endocarditis (ie) have not been standardized. this is especially true with serological testing. patients with suspected ie were evaluated by a standard diagnostic protocol. this protocol mandated an evaluation of the patients according to the modified duke criteria and used a battery of laboratory investigations, including three sets of blood cultures and systematic serological testing f ... | 2005 | 16207989 |
| evaluation of a rapid direct assay for identification of bacteria and the mec a and van genes from positive-testing blood cultures. | we performed the first evaluation of a dna strip assay (genotype blood culture; hain lifescience, nehren, germany) for the detection of the most relevant bacterial sepsis pathogens directly from positive bactec blood culture bottles (becton dickinson, heidelberg, germany). the test comprises two panels, one for the direct species identification of important gram-positive cocci and the other for gram-negative rods. additionally, detection of the mec a and the van genes are implemented. the genoty ... | 2005 | 16207992 |
| evaluation of a new screen agar plate for detection and presumptive identification of enterobacteriaceae producing extended-spectrum beta-lactamases. | a new agar screen plate for extended-spectrum beta-lactamase (esbl) detection was evaluated with 50 clinical isolates of esbl-producing enterobacteriaceae species: enterobacter cloacae (n = 10), escherichia coli (n = 10), klebsiella oxytoca (n = 3), klebsiella pneumoniae (n = 25), and proteus mirabilis (n = 2). fecal samples were artificially inoculated with 2 concentrations (25 and 250 colony forming units [cfu]/plate) of the test strains and then applied to the new agar screen plates. by this ... | 2005 | 15629229 |
| synergy tests by e test and checkerboard methods of antimicrobial combinations against brucella melitensis. | two different synergy testing methods, the checkerboard and the e test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 brucella melitensis strains isolated from blood cultures. the rate of agreement of the e test and checkerboard methods was found to be 55%. the most concordant results were found for the streptomycin-doxycycline combination in 12 (75%) tests, in which four strains showed synergistic activity by e test and antagonistic activi ... | 2005 | 15634962 |
| pcr detection of bacteria on cardiac valves of patients with treated bacterial endocarditis. | we used broad-range pcr amplification and sequencing to detect and identify bacterial dna in 156 valves of patients treated for infective endocarditis (ie). bacterial dna was found more frequently in patients who underwent valve replacement while on antibiotic treatment for ie (60%) than in patients who had completed antibiotic treatment for ie (37%; p = 0.02). we found specific bacterial dna in valves removed from 11 of 30 patients who had completed antibiotic treatment for ie. six had no histo ... | 2005 | 15634966 |
| identification, cloning, and characterization of a lactococcus lactis branched-chain alpha-keto acid decarboxylase involved in flavor formation. | the biochemical pathway for formation of branched-chain aldehydes, which are important flavor compounds derived from proteins in fermented dairy products, consists of a protease, peptidases, a transaminase, and a branched-chain alpha-keto acid decarboxylase (kdca). the activity of the latter enzyme has been found only in a limited number of lactococcus lactis strains. by using a random mutagenesis approach, the gene encoding kdca in l. lactis b1157 was identified. the gene for this enzyme is hig ... | 2005 | 15640202 |
| mutation of a lysr-type regulator of antifungal activity results in a growth advantage in stationary phase phenotype in pseudomonas aureofaciens pa147-2. | the growth advantage in stationary phase (gasp) phenotype was shown to be present in two mutants lacking the antifungal phenotype (af(-) mutants) of pseudomonas aureofaciens pa147-2. complementation demonstrated a correlation between gasp and the antifungal defect in one strain but not in the second. sequence analysis revealed the af(-) gasp strain had a mutation in a gene (finr) encoding a lysr-type regulator. antifungal-minus mutants arose in starved cultures, and those aged cultures had incre ... | 2005 | 15640239 |
| toxicity after prolonged (more than four weeks) administration of intravenous colistin. | the intravenous use of polymyxins has been considered to be associated with considerable nephrotoxicity and neurotoxicity. for this reason, the systemic administration of polymyxins had been abandoned for about 20 years in most areas of the world. however, the problem of infections due to multidrug-resistant (mdr) gram-negative bacteria such us pseudomonas aeruginosa and acinetobacter baumanniii has led to the re-use of polymyxins. our objective was to study the toxicity of prolonged intravenous ... | 2005 | 15642116 |
| gpwac of the t4-type bacteriophages: structure, function, and evolution of a segmented coiled-coil protein that controls viral infectivity. | the wac gene product (gpwac) or fibritin of bacteriophage t4 forms the six fibers that radiate from the phage neck. during phage morphogenesis these whiskers bind the long tail fibers (ltfs) and facilitate their attachment to the phage baseplate. after the cell lysis, the gpwac fibers function as part of an environmental sensing device that retains the ltfs in a retracted configuration and thus prevents phage adsorption in unfavorable conditions. a comparative analysis of the sequences of 5 wac ... | 2005 | 15659683 |
| hydrogen production by rhodobacter sphaeroides strain o.u.001 using spent media of enterobacter cloacae strain dm11. | combined dark and photo-fermentation was carried out to study the feasibility of biological hydrogen production. in dark fermentation, hydrogen was produced by enterobacter cloacae strain dm11 using glucose as substrate. this was followed by a photo-fermentation process. here, the spent medium from the dark process (containing unconverted metabolites, mainly acetic acid etc.) underwent photo-fermentation by rhodobacter sphaeroides strain o.u.001 in a column photo-bioreactor. this combination cou ... | 2005 | 15666144 |
| plasmid-mediated quinolone resistance in isolates obtained in german intensive care units. | screening of 703 isolates of enterobacteriaceae, obtained from 34 german intensive care units (icus), revealed qnr-positive, integron-containing isolates of enterobacter sp. and citrobacter freundii from four patients in 2 german icus. this is one of the first reports of qnr-positive strains obtained from patients in europe. | 2005 | 15673764 |
| detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of enterobacter spp. in hong kong, 2000-2002. | a total of 139 consecutive and non-duplicate bloodstream isolates of enterobacter spp. collected from inpatients in hong kong during 2000-2002 were studied for production of extended-spectrum beta-lactamases (esbls). | 2005 | 15681579 |
| in vitro activity of levofloxacin against recent gram-negative nosocomial pathogens. | the objective of our study was to evaluate the in vitro activity of levofloxacin in comparison with other antibiotics (coamoxiclav, ceftriaxone, ceftazidime, meropenem, aztreonam, ciprofloxacin and netilmicin) against about 500 gram-negative nosocomial microorganisms, isolated during 2003. the bactericidal activities (minimal bactericidal activity and killing curve) of levofloxacin and the other antibiotics were also evaluated. the results confirm the excellent in vitro activity of levofloxacin ... | 2005 | 15767745 |
| variety of tem-, shv-, and ctx-m-type beta-lactamases present in recent clinical isolates of escherichia coli, klebsiella pneumoniae, and enterobacter cloacae from taiwan. | a total of 171 hospitals' isolates of e. coli, k. pneumoniae, and e. cloacae with a minimum inhibitory concentration (mic) of > or =2 microg/ml for ceftazidime or cefotaxime were evaluated for the production of beta-lactamases. pcr amplification with specific primers for the bla (shv), bla (tem), and bla (ctx) genes revealed that a total of 53, 81, and 43 of these genes were amplified, respectively. sequencing results confirmed that tem-1, ctx-m-3 and -14, shv-1, -5, -11, -12, and -33, oxy-1a, a ... | 2005 | 15770092 |
| role of the extracytoplasmic function protein family sigma factor rpoe in metal resistance of escherichia coli. | rpoe of escherichia coli is a sigma factor of the extracytoplasmic function protein family and is required for the expression of proteins involved in maintaining the integrity of periplasmic and outer membrane components. rpoe of e. coli was needed for full resistance to zn(ii), cd(ii), and cu(ii). promoter gene fusion and quantitative real time reverse transcription (rt)-pcr (qrt-pcr) assays demonstrated that expression of rpoe was induced by metals. global gene expression profiles upon metal t ... | 2005 | 15774872 |
| presence of multidrug-resistant enteric bacteria in dairy farm topsoil. | in addition to human and veterinary medicine, antibiotics are extensively used in agricultural settings, such as for treatment of infections, growth enhancement, and prophylaxis in food animals, leading to selection of drug and multidrug-resistant bacteria. to help circumvent the problem of bacterial antibiotic resistance, it is first necessary to understand the scope of the problem. however, it is not fully understood how widespread antibiotic-resistant bacteria are in agricultural settings. th ... | 2005 | 15778307 |
| pilot study for the development of a new campylobacter selective medium at 37 degrees c using aztreonam. | to overcome contamination and temperature inhibition by isolating campylobacter at 37 degrees c. | 2005 | 15790708 |
| efficacy and safety of pharmacokinetically enhanced amoxicillin-clavulanate at 2,000/125 milligrams twice daily for 5 days versus amoxicillin-clavulanate at 875/125 milligrams twice daily for 7 days in the treatment of acute exacerbations of chronic bronchitis. | this randomized, controlled trial was designed to show that a short, 5-day course of pharmacokinetically enhanced amoxicillin-clavulanate at 2,000/125 mg (augmentin xr) is as effective clinically as a longer, 7-day course of conventional amoxicillin-clavulanate at 875/125 mg (both given twice daily) in the treatment of acute exacerbations of chronic bronchitis (aecb). amoxicillin-clavulanate at 2,000/125 mg was designed to extend the therapeutic levels of amoxicillin in serum over the 12-h dosin ... | 2005 | 15616290 |
| comparative antimicrobial characterization of lbm415 (nvp pdf-713), a new peptide deformylase inhibitor of clinical importance. | lbm415 (nvp pdf-713) is the first member of the peptide deformylase (pdf) inhibitor class being developed for clinical trials as a parenteral and oral agent for treatment of community-acquired respiratory tract disease and serious infections caused by antimicrobial-resistant gram-positive cocci. in this study susceptibility testing results from 1,306 recent clinical isolates selected to over-represent resistance trends among the species were summarized. all staphylococci (153 strains; mic at whi ... | 2005 | 15793128 |
| ctx-m-10 linked to a phage-related element is widely disseminated among enterobacteriaceae in a spanish hospital. | ctx-m-10 has been widely disseminated among multiple clones of several species of enterobacteriaceae, harboring seemingly different plasmids, for over a decade in ramón y cajal university hospital, madrid, spain. cloning and sequencing of a 12.2-kb dna fragment from plasmid pryce21 from klebsiella pneumoniae strain kp4ac revealed a novel phage-related element immediately upstream of bla(ctx-m-10) conserved among different ctx-m-10-producing strains. this is the first report showing an extended-s ... | 2005 | 15793141 |
| occurrence of ctx-m-3, ctx-m-15, ctx-m-14, and ctx-m-9 extended-spectrum beta-lactamases in enterobacteriaceae clinical isolates in korea. | among 603 isolates of enterobacteriaceae collected between june and november 2003 from three university hospitals within korea, bla(ctx-m-3), bla(ctx-m-15), bla(ctx-m-14), and bla(ctx-m-9) were detected in 41 isolates of species from five different genera of enterobacteriaceae, escherichia coli, klebsiella pneumoniae, citrobacter freundii, enterobacter spp., and serratia marcescens. | 2005 | 15793142 |
| mechanisms of resistance of bacteria causing ventilator-associated pneumonia. | the common causes of ventilator-associated pneumonia (staphylococcus aureus, pseudomonas aeruginosa, klebsiella pneumoniae, enterobacter cloacae, acinetobacter spp) are frequently resistant to multiple antibiotic classes. s aureus develops resistance to all beta-lactam antibiotics by producing a modified penicillin-binding protein. linezolid resistance arises by way of mutations in the 23s ribosomal subunit. antibiotic resistance in gram-negative bacilli usually arises by way of beta-lactamase p ... | 2005 | 15802168 |
| occurrence of extended-spectrum beta-lactamases among chromosomal ampc-producing enterobacter cloacae, citrobacter freundii, and serratia marcescens in korea and investigation of screening criteria. | we assessed the occurrence and screening criterion for extended-spectrum beta-lactamases (esbls) among ampc-producing enterobacter cloacae, citrobacter freundii, and serratia marcescens. the 413 isolates (158 e. cloacae, 126 c. freundii, and 129 s. marcescens) isolated from 11 clinical laboratories in korea were investigated. esbl production was confirmed by double-disk synergy test and inhibitor-potentiated diffusion test using ceftazidime (caz), cefotaxime (ctx), aztreonam (azt), and cefepime ... | 2005 | 15808318 |
| acquired haemophilia in a patient with gram-negative urosepsis and bladder cancer. | we here report a patient who developed a high titer antibody to factor viii (fviii) during gram-negative urosepsis caused by enterobacter cloacae after complete resection of rectal cancer. the patient initially presented with a life threatening spontaneous hematothorax and multiple large haematomas. coagulation studies revealed a severe fviii deficiency <1% with a high fviii antibody titer of 64 bu. the bleeding responded rapidly to infusions of recombinant factor viia. after achievement of a pa ... | 2005 | 15810923 |
| azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis. | an antimicrobial compound was isolated from azospirillum brasilense culture extracts by high-performance liquid chromatography and further identified by gas chromatography-mass spectrometry as the auxin-like molecule, phenylacetic acid (paa). paa synthesis was found to be mediated by the indole-3-pyruvate decarboxylase, previously identified as a key enzyme in indole-3-acetic acid (iaa) production in a. brasilense. in minimal growth medium, paa biosynthesis by a. brasilense was only observed in ... | 2005 | 15812004 |
| quantitative detection of listeria monocytogenes in biofilms by real-time pcr. | a quantitative method based on a real-time pcr assay to enumerate listeria monocytogenes in biofilms was developed. the specificity for l. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a sybr green i real-time pcr assay. the number of l. monocytogenes detected growing in biofilms was 6 x 10(2) cfu/cm2. | 2005 | 15812058 |
| identification of clinically relevant viridans streptococci by an oligonucleotide array. | viridans streptococci (vs) are common etiologic agents of subacute infective endocarditis and are capable of causing a variety of pyogenic infections. many species of vs are difficult to differentiate by phenotypic traits. an oligonucleotide array based on 16s-23s rrna gene intergenic spacer (its) sequences was developed to identify 11 clinically relevant vs. these 11 species were streptococcus anginosus, s. constellatus, s. gordonii, s. intermedius, s. mitis, s. mutans, s. oralis, s. parasangui ... | 2005 | 15814960 |
| endotoxic shock after gamete intrafallopian transfer. | to report an extremely rare case of endotoxic shock due to enterobacter cloacae that occurred after laparoscopy for gamete intrafallopian transfer (gift) in a nulligravid woman. | 2005 | 15820819 |
| hemoglobin conformation couples erythrocyte s-nitrosothiol content to o2 gradients. | it is proposed that the bond between nitric oxide (no) and the hb thiol cys-beta(93) (snohb) is favored when hemoglobin (hb) is in the relaxed (r, oxygenated) conformation, and that deoxygenation to tense (t) state destabilizes the snohb bond, allowing transfer of no from hb to form other (vasoactive) s-nitrosothiols (snos). however, it has not previously been possible to measure snohb without extensive hb preparation, altering its allostery and sno distribution. here, we have validated an assay ... | 2005 | 15824313 |
| metallo-beta-lactamases: the quiet before the storm? | the ascendancy of metallo-beta-lactamases within the clinical sector, while not ubiquitous, has nonetheless been dramatic; some reports indicate that nearly 30% of imipenem-resistant pseudomonas aeruginosa strains possess a metallo-beta-lactamase. acquisition of a metallo-beta-lactamase gene will invariably mediate broad-spectrum beta-lactam resistance in p. aeruginosa, but the level of in vitro resistance in acinetobacter spp. and enterobacteriaceae is less dependable. their clinical significan ... | 2005 | 15831827 |
| isolation of silver- and antibiotic-resistant enterobacter cloacae from teeth. | antibiotic-resistant bacteria pose a serious threat to human health; hence the mechanisms that lead to their selection need to be investigated. one possible mechanism is that the silver and mercury in amalgam dental restorations may select for bacteria that contain heavy metal and antibiotic-resistance determinants, leading to the spread of these resistances, particularly if they are contained on the same mobile genetic element. the incidence of silver-resistant bacteria on teeth is investigated ... | 2005 | 15836522 |
| genetic environment and expression of the extended-spectrum beta-lactamase blaper-1 gene in gram-negative bacteria. | the genetic location of the gene coding for the expanded-spectrum beta-lactamase per-1 was analyzed in a series of gram-negative isolates. it was identified as part of a composite transposon bracketed by two novel insertion elements, ispa12 and ispa13, belonging to the is4 family that possess transposases that share 63% amino acid identity and that are chromosomally located in pseudomonas aeruginosa, providencia stuartii, and acinetobacter baumannii. on the contrary, the bla(per-1) gene was iden ... | 2005 | 15855485 |
| evaluation by monte carlo simulation of the pharmacokinetics of two doses of meropenem administered intermittently or as a continuous infusion in healthy volunteers. | meropenem is a broad-spectrum carbapenem antibacterial agent. in order to optimize levels in plasma relative to the mics, the ideal dose level and dosage regimen need to be determined. the pharmacokinetics of meropenem were studied in two groups, each comprising eight healthy volunteers who received the following doses: 500 mg as an intravenous infusion over 30 min three times a day (t.i.d.) versus a 250-mg loading dose followed by a 1,500 mg continuous infusion over 24 h for group a and 1,000 m ... | 2005 | 15855510 |
| further identification of plasmid-mediated quinolone resistance determinant in enterobacteriaceae in turkey. | 2005 | 15855552 | |
| the iron-siderophore transporter fhua is the receptor for the antimicrobial peptide microcin j25: role of the microcin val11-pro16 beta-hairpin region in the recognition mechanism. | the role of the outer-membrane iron transporter fhua as a potential receptor for the antimicrobial peptide mccj25 (microcin j25) was studied through a series of in vivo and in vitro experiments. the requirement for both fhua and the inner-membrane tonb-exbb-exbd complex was demonstrated by antibacterial assays using complementation of an fhua(-) strain and by using isogenic strains mutated in genes encoding the protein complex respectively. in addition, mccj25 was shown to block phage t5 infecti ... | 2005 | 15862112 |
| chemical forms of selenium in the metal-resistant bacterium ralstonia metallidurans ch34 exposed to selenite and selenate. | ralstonia metallidurans ch34, a soil bacterium resistant to a variety of metals, is known to reduce selenite to intracellular granules of elemental selenium (se(0)). we have studied the kinetics of selenite (se(iv)) and selenate (se(vi)) accumulation and used x-ray absorption spectroscopy to identify the accumulated form of selenate, as well as possible chemical intermediates during the transformation of these two oxyanions. when introduced during the lag phase, the presence of selenite increase ... | 2005 | 15870319 |
| prevalence of derepressed ampc mutants and extended-spectrum beta-lactamase producers among clinical isolates of citrobacter freundii, enterobacter spp., and serratia marcescens in korea: dissemination of ctx-m-3, tem-52, and shv-12. | the resistance mechanism of extended-spectrum cephalosporins in clinical isolates of citrobacter freundii, enterobacter spp., and serratia marcescens was studied. of 152 isolates, 45 isolates (29.6%) were derepressed ampc mutants and 39 isolates (25.7%) produced extended-spectrum beta-lactamase (esbls). the most prevalent esbls were ctx-m enzymes, followed by tem-52 and shv-12. | 2005 | 15872281 |
| practical application of dynamic temperature profiles to estimate the parameters of the square root model. | optimal experimental design for parameter estimation (oed/pe) is a promising method to improve parameter estimation accuracy and minimise experimental effort in the field of predictive microbiology. in this paper, the oed/pe methodology was applied on two practical examples: the growth of bacillus cereus and enterobacter cloacae in liquid whole egg product. both strains were recovered from samples of a commercial product. the goal of the modelling exercise was to quantify the influence of temper ... | 2005 | 15878409 |
| gram-negative bacteria from the camel tick hyalomma dromedarii (ixodidae) and the chicken tick argas persicus (argasidae) and their antibiotic sensitivities. | a total of nine species of gram-negative bacteria were isolated from organs and haemolymph of the hard tick hyalomma (hyalomma) dromedarii and the soft tick argas (persicargas) persicus. four species namely serratia liquefaciens, stenotrophomonas maltophilia, klebsiella ornithinolytica and aeromonas hydrophila were isolated from h. dromedarii and five species namely rahnella aquatilis, pseudomonas fluorescens, enterobacter cloacae, chryseomonas luteola and chryseobacterium meningosepticum were i ... | 2005 | 15880998 |
| pore formation, polymerization, hemolytic and leukotoxic effects of a new enterobacter cloacae toxin neutralized by antiserum. | a new toxin of enterobacter cloacae was purified and studied by sds-page electrophoresis with the purpose of investigating its ability to generate polymers and their molecular mass. monomer of 13.3 kda and structures of multimeric mass were detected. the toxin of 66 kda was the most abundant form of toxin. this polymer and the monomer were selected to examine blood cells damage. membrane pores caused by both toxin forms seemed to be of similar dimension (estimated in 3.6 nm by experiments with o ... | 2005 | 15881838 |
| bacteremia in patients with hematological malignancies. | an appropriate regimen in the empirical therapy of neutropenic fever episodes must be individualized at each institution. hospitals have different patterns of microbial isolates and antibiotic resistance that must be taken into account. the aim of this study was to investigate isolates of bacteremia and their antibiotic susceptibility in patients with hematological malignancies. | 2005 | 15886475 |
| the d-methyl group in beta-lactamase evolution: evidence from the y221g and gc1 mutants of the class c beta-lactamase of enterobacter cloacae p99. | the beta-lactam antibiotics act through their inhibition of d-alanyl-d-alanine transpeptidases (dd-peptidases) that catalyze the last step of bacterial cell wall synthesis. bacteria resist beta-lactams by a number of mechanisms, one of the more important of which is the production of beta-lactamases, enzymes that catalyze the hydrolysis of these antibiotics. the serine beta-lactamases are evolutionary descendants of dd-peptidases and retain much of their structure, particularly at the active sit ... | 2005 | 15895997 |
| characterization of an exopolysaccharide produced by a marine enterobacter cloacae. | an exopolysaccharide producing marine bacterium, enterobacter cloacae, was isolated from marine sediment collected from gujarat coast, india. chemical investigation of exopolysaccharide (eps 71 a) revealed that this exopolysaccharide was an acidic polysaccliaride containing high amount of uronic acid, fucose and sulfate which is rare for bacterial exopolysaccharides. eps 71a was found to have fucose, galactose, glucose and glucuronic acid in a molar ratio of 2: 1: 1: 1. | 2005 | 15900914 |
| reassignment of enterobacter dissolvens to enterobacter cloacae as e. cloacae subspecies dissolvens comb. nov. and emended description of enterobacter asburiae and enterobacter kobei. | the taxonomic position of enterobacter dissolvens was re-evaluated based on the analysis of the type strain atcc 23373t and three clinical isolates. the strains were assigned to the genetic cluster of the species by phylogenetic sequence analysis in the frame of a recent population genetic study. the relatedness of e. dissolves to the other species of the e. cloacae complex was analyzed by dna-dna hybridization studies based on melting profiles in microplates. the genetic cluster of e. dissolven ... | 2005 | 15900966 |
| description of enterobacter ludwigii sp. nov., a novel enterobacter species of clinical relevance. | a new species, enterobacter ludwigii, is presented on the basis of the characteristics of 16 strains, which were isolated from clinical specimens. these bacteria form a distinct genetic cluster in phylogenetic analyses of the population structure of the enterobacter cloacae complex. as determined by dna-dna cross-hybridization experiments in microplates, this genetic cluster can be delineated from the other species of the e. cloacae complex with deltatm values equal to or above 5 degrees c with ... | 2005 | 15900967 |
| enterobacter radicincitans sp. nov., a plant growth promoting species of the family enterobacteriaceae. | a plant growth promoting bacterial isolate (d5/23t) from the phyllosphere of winter wheat, able to fix atmospheric nitrogen and to produce auxines and cytokinins was investigated in a polyphasic taxonomy approach. phylogenetic analyses using the 16s rrna gene sequence of the strain clearly indicated that the strain belonged to the family enterobacteriaceae, most closely related to enterobacter cloacae with 99.0% and enterobacter dissolvens with 98.5% sequence similarity. phylogenetic analysis de ... | 2005 | 15900968 |
| purification and characterization of allophanate hydrolase (atzf) from pseudomonas sp. strain adp. | atzf, allophanate hydrolase, is a recently discovered member of the amidase signature family that catalyzes the terminal reaction during metabolism of s-triazine ring compounds by bacteria. in the present study, the atzf gene from pseudomonas sp. strain adp was cloned and expressed as a his-tagged protein, and the protein was purified and characterized. atzf had a deduced subunit molecular mass of 66,223, based on the gene sequence, and an estimated holoenzyme molecular mass of 260,000. the acti ... | 2005 | 15901697 |
| cryptic plasmid prk2 from escherichia coli w: sequence analysis and segregational stability. | cryptic plasmid prk2 of the strain escherichia coli w (atcc 9637), an ancestor of production strains for penicillin g acylase, was sequenced and characterized. based on the data on replication region and origin (ori sequence aac, 924-926nt), the plasmid was classified as cole1-like plasmid. dna sequence analysis revealed five orfs hypothetical products of which shared a significant sequence similarity with putative proteins encoded by dna of plasmid pcole1. orf1 codes for protein rom involved in ... | 2005 | 15907542 |
| bacterial cell-to-cell signaling in the gastrointestinal tract. | 2005 | 15908344 | |
| respiratory pathogens in dental plaque of hospitalized patients with chronic lung diseases. | bacterial cultivation studies have shown that dental plaque is a reservoir for respiratory pathogens in intensive care unit patients and in elderly who are debilitated, hospitalized or in a nursing home, placing them at risk of bacterial pneumonia. no information is available, however, concerning dental plaque as a reservoir of putative respiratory pathogens in hospitalized patients with chronic lung diseases. supragingival plaque colonization of 34 hospitalized chronic lung-diseased romanian ci ... | 2005 | 15909174 |
| the rising influx of multidrug-resistant gram-negative bacilli into a tertiary care hospital. | the prevalence of multidrug resistance (mdr) among gram-negative bacilli is rapidly increasing. quantification of the prevalence and the common antimicrobial coresistance patterns of mdr gram-negative bacilli (mdr-gnb) isolates recovered from patients at hospital admission, as well as identification of patients with a high risk of harboring mdr-gnb, would have important implications for patient care. | 2005 | 15909268 |
| temporal changes in bacterial resistance in german intensive care units, 2001-2003: data from the sari (surveillance of antimicrobial use and antimicrobial resistance in intensive care units) project. | conservative testing revealed a stable antibiotic resistance situation for staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae and enterobacter cloacae in 32 german intensive care units (icus) actively participating in the sari (surveillance of antimicrobial use and antimicrobial resistance in icus) project over a three-year period (2001--2003). no significant changes were shown for methicillin-resistant s. aureus (mrsa) (p=0.501; the mrsa rate increased in 18 ... | 2005 | 15923060 |
| efficacy and safety of telithromycin 800 mg once daily for 7 days in community-acquired pneumonia: an open-label, multicenter study. | community-acquired pneumonia (cap) remains a major cause of morbidity and mortality throughout the world. telithromycin (a new ketolide) has shown good in vitro activity against the key causative pathogens of cap, including s pneumoniae resistant to penicillin and/or macrolides. | 2005 | 15927060 |
| evolutionary relationships of three new species of enterobacteriaceae living as symbionts of aphids and other insects. | ecological studies on three bacterial lineages symbiotic in aphids have shown that they impose a variety of effects on their hosts, including resistance to parasitoids and tolerance to heat stress. phylogenetic analyses of partial sequences of gyrb and reca are consistent with previous analyses limited to 16s rrna gene sequences and yield improved confidence of the evolutionary relationships of these symbionts. all three symbionts are in the enterobacteriaceae. one of the symbionts, here given t ... | 2005 | 15933033 |
| microbial contamination of hematopoietic progenitor cell grafts-incidence, clinical outcome, and cost-effectiveness: an analysis of 735 grafts. | screening of progenitor cell grafts (marrow, peripheral blood, and cord blood) for microbial contamination is required by the standards of aabb. clinical sequelae from infusion of these contaminated grafts, however, is uncommon. | 2005 | 15934984 |
| enterobacter meningitis: organism susceptibilities, antimicrobial therapy and related outcomes. | meningitis due to enterobacter species is an uncommon infection in adults; however, when present, treatment is frequently complicated by resistance of many enterobacter isolates to third-generation cephalosporins and poor central nervous system penetration of other antibiotics. the aim of this study was to retrospectively review cases of meningitis caused by enterobacter species at our institution, to better characterize patient factors, pathogen characteristics, and treatment options for this i ... | 2005 | 15936376 |
| [an investigation of bacterial ecology and analysis of bacterial resistance to antibiotics in a burn ward in nanning district]. | to investigate the changes in the bacterial ecology and to analyze the bacterial resistance to antibiotics in a burn ward in nanning district during the past 15 years, so as to provide reference to the clinical management of burn infection under subtropical climate. | 2005 | 15938956 |
| practical methods using boronic acid compounds for identification of class c beta-lactamase-producing klebsiella pneumoniae and escherichia coli. | detection of the resistance mediated by class c beta-lactamases remains a challenging issue, considering that transferable plasmid-mediated class c beta-lactamases are of worldwide concern. methods for the identification of strains that produce extended-spectrum beta-lactamases (esbls) or metallo-beta-lactamases (mbls) have been developed and applied for routine use in clinical microbiology laboratories, but no practical methods for identification of plasmid-mediated class c producers have been ... | 2005 | 15956362 |
| gene mutations responsible for overexpression of ampc beta-lactamase in some clinical isolates of enterobacter cloacae. | ampc regulatory genes in 21 ceftazidime-resistant clinical isolates of enterobacter cloacae (mics of > or = 16 microg/ml) were characterized. all isolates exhibited ampc overproduction due to ampd mutation. additionally, we found two ampr mutants among the isolates. this is the first report of chromosomal ampr mutation in clinical isolates of e. cloacae. | 2005 | 15956430 |
| first identification of clostridium celerecrescens in liquid drained from an abscess. | we present the first report on the characterization of clostridium celerecrescens isolated from liquid drained via abscess puncture from a 45-year-old man. the organism was identified by sequence comparison of 16s rrna genes. | 2005 | 15956446 |
| outbreak of infection caused by enterobacter cloacae producing the novel veb-3 beta-lactamase in china. | over a 4-month period from november 2002 to february 2003, 27 ceftazidime-resistant or cefotaxime-resistant nonrepetitive enterobacter cloacae isolates were collected from 27 patients hospitalized at huashan hospital, shanghai, people's republic of china. the etest did not detect extended-spectrum beta-lactamases (esbls) in those 27 isolates; however, screening by the nccls esbl disk test and confirmatory tests detected esbls in 4 of 27 isolates and pcr detected esbls in 23 of 27 isolates. the m ... | 2005 | 15695687 |
| evaluation of etest mbl for detection of blaimp-1 and blavim-2 allele-positive clinical isolates of pseudomonas spp. and acinetobacter spp. | the etest mbl (ab biodisk, solna, sweden) correctly differentiated all 57 isolates of acinetobacter spp. and pseudomonas aeruginosa with the bla(imp-1) allele and 135 of 137 (98.5%) acinetobacter spp. and pseudomonas spp. isolates with the bla(vim-2) allele. the etest mbl was reliable for detecting the imp-1- and vim-2-producing pseudomonas and acinetobacter isolates. | 2005 | 15695713 |
| the effect of an antimicrobial drain sponge dressing on specific bacterial isolates at tracheostomy sites. | patients with tracheostomies frequently experience complications, including bacteremia, sepsis, pneumonia, and multi antibiotic-resistant bacterial infections. a prospective, descriptive, randomized, controlled, clinical case series involving seven men and three women was conducted on patients in the neuroscience unit of a long-term rehabilitation hospital during a period of 25 days to compare the use of an nonwoven drain sponge dressing containing an antimicrobial (polyhexamethylene biguanide) ... | 2005 | 15695836 |
| [distribution and drug-resistance of 3 500 gram-negative bacteria in guangzhou]. | to investigate the distribution and drug-resistance of the common gram-negative bacteria in guangzhou. | 2005 | 15698988 |
| detection of virulence genes in malaysian shigella species by multiplex pcr assay. | in malaysia, shigella spp. was reported to be the third commonest bacterial agent responsible for childhood diarrhoea. currently, isolation of the bacterium and confirmation of the disease by microbiological and biochemical methods remain as the "gold standard". this study aimed to detect the prevalence of four shigella virulence genes present concurrently, in randomly selected malaysian strains via a rapid multiplex pcr (mpcr) assay. | 2005 | 15707504 |
| interactions of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes plants cultivated in a gnotobiotic system. | the growth and persistence of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes on a diverse range of plant types over extended cultivation periods was studied. when introduced on the seed of carrot, cress, lettuce, radish, spinach and tomato all the pathogens became rapidly established shortly after germination, attaining cell densities of the order of 5.5-6.5 log cfu/g. in general, es. coli o157:h7 and l. monocytogenes became established and persisted at significantly ... | 2005 | 15718025 |
| influence of transcriptional activator rama on expression of multidrug efflux pump acrab and tigecycline susceptibility in klebsiella pneumoniae. | tigecycline is an expanded broad-spectrum antibacterial agent that is active against many clinically relevant species of bacterial pathogens, including klebsiella pneumoniae. the majority of k. pneumoniae isolates are fully susceptible to tigecycline; however, a few strains that have decreased susceptibility have been isolated. one isolate, g340 (for which the tigecycline mic is 4 microg/ml and which displays a multidrug resistance [mdr] phenotype), was selected for analysis of the mechanism for ... | 2005 | 15728897 |
| extended-spectrum beta-lactamases among enterobacter isolates obtained in tel aviv, israel. | the extended-spectrum beta-lactamase (esbl)-producing phenotype is frequent among enterobacter isolates at the tel aviv sourasky medical center, tel aviv, israel. we examined the clonal relatedness and characterized the esbls of a collection of these strains. clonal relatedness was determined by pulsed-field gel electrophoresis. isoelectric focusing (ief) and transconjugation experiments were performed. esbl gene families were screened by colony hybridization and pcr for bla(tem), bla(shv), bla( ... | 2005 | 15728917 |
| clostridium bacteraemia characterised by 16s ribosomal rna gene sequencing. | owing to problems in accurate species identification of the diverse genus clostridium, the epidemiology and pathogenicity of many species are not fully understood. moreover, previous studies on clostridium bacteraemia have been limited and relied only on phenotypic species identification. | 2005 | 15735165 |
| genetically engineered termite gut bacteria (enterobacter cloacae) deliver and spread foreign genes in termite colonies. | indigenous gut bacteria of the formosan subterranean termite (coptotermes formosanus shiraki, isoptera: rhinotermitidae) were used as shuttle systems to deliver, express and spread foreign genes in termite colonies. the gut bacterium enterobacter cloacae was transformed with a recombinant plasmid (pegfp) containing genes encoding ampicillin resistance and green fluorescent protein (gfp). in laboratory experiments, termite workers and soldiers from three colonies were fed with filter paper inocul ... | 2005 | 15742168 |
| isolation and characterization of novel giant stenotrophomonas maltophilia phage phisma5. | stenotrophomonas maltophilia is one of the most prevalent opportunistic bacteria causing nosocomial infections. it has become problematic because most of the isolates are resistant to multiple antibiotics, and therefore, development of phage therapy has attracted strong attention. in this study, eight s. maltophilia phages were isolated from clinical samples including patient specimens, catheter-related devices, and wastewater. these phages can be divided into four distinct groups based on host ... | 2005 | 15746341 |
| osteomyelitis caused by enterobacter cancerogenus infection following a traumatic injury: case report and review of the literature. | we report a case of osteomyelitis caused by enterobacter cancerogenus resistant to aminopenicillins in a 56-year-old male who had a motorcycle accident and suffered from multiple bone fractures with abundant environmental exposure. e. cancerogenus has rarely been associated with human infections, and its clinical significance remains unclear. | 2005 | 15750133 |