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bacillus subtilis spores reduce susceptibility to citrobacter rodentium-mediated enteropathy in a mouse model.the present work was aimed at investigating whether bacillus subtilis spores, widely used in probiotic as well as pharmaceutical preparations for mild gastrointestinal disorders, can suppress enteric infections. to address this issue, we developed a mouse model of infection using the mouse enteropathogen citrobacter rodentium, a member of a family of human and animal pathogens which includes the clinically significant enteropathogenic (epec) and enterohemorrhagic (ehec) escherichia coli strains. ...200617005378
mercuric reductase gene transfer from soil to rumen bacteria.conjugal transfer between soil bacterial population and microorganisms isolated from the rumen of herbivores from mercury-polluted area was investigated. the transfer of mera encoding mercury-resistance plasmids from soil bacteria enterobacter cloacae and enterococcus durans into two ruminal isolates citrobacter freundii and bacillus subtilis was observed. approximately the same frequency of mobilization in mating experiments was observed for both gram-negative (approximately 2.5 x 10(-8), trans ...200617007435
emergence of serine carbapenemases (kpc and sme) among clinical strains of enterobacteriaceae isolated in the united states medical centers: report from the mystic program (1999-2005).among 8885 enterobacteriaceae tested in the 1999 to 2005 period as part of the usa meropenem yearly susceptibility test information collection (mystic) program, 51 strains with increased imipenem and meropenem mic values (> or =2 microg/ml) were detected. bla(kpc) was identified from 28 klebsiella pneumoniae from 3 medical centers in the new york city area (8 ribotypes), 2 klebsiella oxytoca from arkansas (same ribotype), 7 citrobacter freundii (6 from new york [5 ribotypes] and 1 from delaware) ...200617020798
comparison of colonization dynamics and pathology of mice infected with enteropathogenic escherichia coli, enterohaemorrhagic e. coli and citrobacter rodentium.enteropathogenic escherichia coli (epec), enterohaemorrhagic e. coli (ehec) and citrobacter rodentium (cr) colonize the gastrointestinal tract epithelium via attaching and effacing lesions. while humans are believed to be the only living reservoir of typical epec and ehec to have border host specificity, cr is a restricted mouse pathogen. recently, conflicting conclusions were reported concerning the utility of a murine model to study mechanisms of epec and ehec colonization and infection. we th ...200617034412
a novel phytase with preferable characteristics from yersinia intermedia.a yersinia intermedia strain producing phytase was isolated from glacier soil. the phytase gene, appa, was isolated by degenerate pcr and tail-pcr. the full-length fragment contained 2354bp with a 1326-bp open reading frame encoding 441 amino acids. appa contained the active site rhgxrxp and hd sequence motifs that are typical of histidine acid phosphatases. to our knowledge, this is the first report of the detection of phytase activity and cloning of the relevant gene from y. intermedia. the ge ...200617034758
evaluation of the 10th external quality assessment scheme results in clinical microbiology laboratories in tehran and districts.we evaluated the performance of microbiology laboratories in the 10th run of the external quality assessment scheme (eqas) in tehran and districts. each laboratory was sent 2 species of bacteria for identification. of the 487 laboratories that participated, 437 returned their findings. while 77.0% and 69.9% correctly identified staphylococcus saprophyticus and citrobacter freundii respectively, only 29.8% correctly identified acinetobacter baumanii, 25.3% identified enterococcus faecalis and 35. ...200617037699
carbon : nitrogen : phosphorus ratios influence biofilm formation by enterobacter cloacae and citrobacter freundii.to test the effects of c : n : p ratio modification of a well-known nutrient medium formulation, the endo formulation on biofilm formation by enterobacter cloacae ecl and citrobacter freundii cf1 in both single-species and binary species biofilms.200617040234
[development of beta-lactamase resistance in enterobacteria].enterobacteria produce elementary chromosomal enzymes, beta-lactamases of class a: tem and shv (escherichia coli, klebsiella pneumoniae). these can give rise to plasmid-coded broad-spectrum beta-lactamases (esbl) discovered in 1980 (e. coli, k. pneumoniae, enterobacter cloacae). the first cefotaximase (ctx-m, men-1) was reported in europe in 1990. this enzyme is far more active against cefotaxime than against ceftazidime and aztreonam. chromosomal hyperpoduction of k1 beta-lactamase differs from ...200617051471
animal models of inflammatory bowel disease: lessons from enteric infections.mouse models of intestinal inflammation have played a key role in understanding the mechanisms that govern the inflammatory response in the intestine, and in designing new therapeutic strategies in the treatment of patients with inflammatory bowel disease (ibd). most of these models use chemical challenges, whereas relatively few robust models of intestinal inflammation caused by microbial infection are known. two common models of infectious murine colitis and typhlitis are infection with the mu ...200617057188
role of lymphotoxins in the development of peyer's patches and mesenteric lymph nodes: relevance to intestinal inflammation and treatment.hallmarks of the adaptive immune system are antigen-specific cellular and humoral immune responses. secondary lymphoid organs serve as sites of contact between antigen-presenting cells (apcs) and immune effector t and b lymphocytes. the gut-associated lymphatic system (galt) as the intestinal branch of the immune system provides different mechanisms to protect organisms against pathogens. simultaneously, immune activation secondary to genetic factors and/or environmental signals can induce detri ...200617057199
identification of nif genes in n2-fixing bacterial strains isolated from rice fields along the yangtze river plain.the aim of this research was to identify nifh and nifhdkye ' genes in twenty strains of n2-fixing heterotrophic bacteria isolated from rice fields in the yangtze river plain. southern hybridization of the total dna from each strain was performed with the klebsiella pneumoniae nifhdkye ' gene probe (6.2 kb eco ri fragment from psa30) and the azospirillum brasilense nifh gene probe (0.6 kb eco ri-hin diii fragment from phu8). we found that eco ri fragments of total dna from aeromonas hydrophila hy ...200616463319
microbial quality of commercial 'golden delicious' apples throughout production and shelf-life in lleida (catalonia, spain).'golden delicious' apples taken from different points throughout the production chain and shelf-life were analysed for enterobacteriaceae, thermotolerant coliforms, e. coli, e. coli o157:h7 and salmonella spp. a total of 36 samples picked up in the orchard, 36 after storage and handling in the packinghouses and 144 from different retail stores were analysed using standardised techniques. enterobacteriaceae counts were very variable. pantoea spp. was the main genera isolated. the percentage of sa ...200616488042
nosocomial infections at kenyatta national hospital intensive-care unit in nairobi, kenya.the objective of this study was to identify the common bacteria isolated from patients, antibiotics used, sensitivity patterns, therapeutic procedures and cleaning protocols practised in kenyatta national hospital intensive-care unit (icu). kenyatta national hospital is a 1,800-bed referral and tertiary-care hospital which is also the teaching university hospital. the icu has 20 beds. two members of staff, a consultant and a senior nurse, did the study. out of 195 patients admitted to the unit d ...200616490968
microbial pattern and antimicrobial resistance, a surgeon's perspective: retrospective study in surgical wards and seven intensive-care units in two university hospitals in cairo, egypt.significant morbidity and mortality in surgical practice is due to infection with resistant pathogens. data from egyptian hospitals may reflect a peculiar pattern.200616490969
a nosocomial outbreak due to novel ctx-m-2-producing strains of citrobacter koseri in a hematological ward. 200616495644
evaluation of a new chromogenic medium for isolation and identification of common urinary tract pathogens.in the study presented here, a new chromogenic medium (cps id 3; biomérieux, marcy l'etoile, france) was compared to routine media for the isolation, identification and antimicrobial susceptibility testing of bacteria recovered from urine specimens, and a cost analysis was performed. escherichia coli, proteeae tribe, pseudomonas aeruginosa, enterococcus spp. and streptococcus agalactiae grew on the chromogenic medium as typical differentiated colonies and were accurately identified even in mixed ...200616496114
small substrate, big surprise: fold, function and phylogeny of dihydroxyacetone kinases.dihydroxyacetone (dha) kinases are a family of sequence-conserved enzymes which utilize either atp (in animals, plants and eubacteria) or phosphoenolpyruvate (pep, in eubacteria) as their source of high-energy phosphate. the kinases consist of two domains/subunits: dhak, which binds dha covalently in hemiaminal linkage to the nepsilon2 of a histidine, and dhal, an eight-helix barrel that contains the nucleotide-binding site. the pep-dependent kinases comprise a third subunit, dham, which rephosp ...200616505971
comparative analysis of a modified ecolite method, the colicomplete method, and a most-probable-number method for detecting escherichia coli in orange juice.the ecolite high volume juice (hvj) presence-absence method for a 10-ml juice sample was compared with the u.s. food and drug administration bacteriological analytical manual most-probable-number (mpn) method for analysis of artificially contaminated orange juices. samples were added to ecolite-hvj medium and incubated at 35 degrees c for 24 to 48 h. fluorescent blue results were positive for glucuronidase- and galactosidase-producing microorganisms, specifically indicative of about 94% of esche ...200616416921
carriage by the german cockroach (blattella germanica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospitals and households in tripoli, libya.using standard bacteriological procedures, 403 cockroaches (blattella germanica) collected in tripoli, from hospitals or the households surrounding the hospitals, were examined for bacteria that are potentially pathogenic to humans. almost all of the cockroaches (96.1% of the 253 from hospitals and 98.7% of the 150 from households) were found to be carrying potentially pathogenic bacteria, with similar mean burdens of 3.2 x 10(5) colony-forming units (cfu) (range=0-1.4 x 10(7)) for each hospital ...200616417714
[beta-lactamases of gram negative bacteria: never-ending clockwork!].the acquired resistance against the wide-spectrum and highly stable beta-lactams including third-generation cephalosporins (3gc) and carbapenems is constinuously increasing and widespead with the discovery of various plasmid-encoded, or genes cassette or integrons coding for a novel beta-lactamase, always a major mechanism of resistance. to explain resistance against 3gc, with the continuing story with tem and shv mutated enzymes, several types of esbl (class a) emerge the ctx-m type, at least c ...200616420989
characterization of the chromosomal class a beta-lactamase cko from citrobacter koseri.the gene bla(cko) encoding the chromosomal class a beta-lactamase of citrobacter koseri was cloned and sequenced. cko was found to display only 41% identity with sed-1 from citrobacter sedlakii and 36% with cdia from citrobacter amalonaticus (formerly citrobacter diversus). no transcriptional regulator was found upstream from bla(cko). silent and missense mutations were detected in four bla(cko) genes amplified from different c. koseri clinical isolates, but the cko variants displayed identical ...200616445758
temporal association of entomopathogenic nematodes (rhabditida: steinernematidae and heterorhabditidae) and bacteria.galleria mellonella l. larvae were infected with three species (seven strains) of steinernema spp. or three species (three strains) of heterorhabditis spp. infected larvae were incubated at 22, 27, and 32 degrees c. larvae were dorsally dissected every 6h over a 48-h period. hemolymph was collected and streaked on tryptic soy agar plates. several non-symbiotic bacterial species were identified from infected insect cadavers: enterobacter gergoviae, vibrio spp., pseudomonas fluorescens type c, ser ...200616448667
isolation of salmonella bacteriophages from swine effluent lagoons.bacteriophages (phages) associated with salmonella were collected from nine swine manure lagoons in mississippi. phages were isolated by an enrichment protocol or directly from effluent. for enrichment, chloroform-treated samples were filtered (0.22 mum) and selectively enriched by adding a cocktail of salmonella strains in trypticase soy broth. after overnight incubation at 35 degrees c, chloroform was added and samples stored at 5 degrees c. enriched samples were tested by double agar layer (d ...200616455853
colonic bacterial infection abrogates eosinophilic pulmonary disease.induction of immunity to one pathogen in the lungs modifies the microenvironment and alters immunopathological changes that result from a second, unrelated pulmonary infection. however, it is unclear whether immunity generated at distant sites also affects lung immune responses. here, we show that infection with the gut-restricted bacterium citrobacter rodentium modifies immunopathological changes that result from pulmonary cryptococcus neoformans infection. th2 cytokine-driven pulmonary eosinop ...200616362886
helminth-primed dendritic cells alter the host response to enteric bacterial infection.to examine whether intestinal helminth infection may be a risk factor for enteric bacterial infection, a murine model was established using the intestinal helminth heligomosomoides polygyrus and a murine pathogen citrobacter rodentium, which causes infectious colitis. using this model we recently have shown that coinfection with the th2-inducing h. polygyrus and c. rodentium promotes bacterial-associated disease and colitis. in this study, we expand our previous observations and examine the hypo ...200616365440
the lpf gene cluster for long polar fimbriae is not involved in adherence of enteropathogenic escherichia coli or virulence of citrobacter rodentium.using the enteropathogenic escherichia coli (epec) genome sequence, we found that epec e2348/69 has an lpfabcde gene cluster homologous (about 60% identical at the protein level) to the salmonella long polar fimbria (lpf) operon. to determine whether this operon is essential for adherence, the lpfabcd(e2)(3) genes were deleted from epec strain e2348/69 by allelic exchange. analysis of the resulting epecdeltalpfabcd(e23) strain showed no change in adherence to hela cells or to human intestinal bi ...200616368980
cd4+-t-cell effector functions and costimulatory requirements essential for surviving mucosal infection with citrobacter rodentium.citrobacter rodentium causes an attaching and effacing infection of the mouse colon. surprisingly, protective adaptive immunity against this mucosal pathogen requires a systemic t-cell-dependent antibody response. to define cd4+ t-cell effector functions promoting this systemic defense of infected epithelial surfaces, studies were undertaken in weaning-age mice lacking costimulatory molecules cd28 or cd40l or cytokines gamma interferon (ifn-gamma) or interleukin-4 (il-4). adoptive transfer of cd ...200616369024
molecular cloning of the phytase gene from citrobacter braakii and its expression in saccharomyces cerevisiae.the gene, appa, encoding phytase was cloned from a size-selected genomic library of citrobacter braakii yh-15 by southern hybridization using a degenerate probe based on the n-terminal amino acid sequence of the phytase. the deduced amino acid sequence of appa contained the n-terminal rhgxrxp motif and the c-terminal hd motif, which are common in histidine acid phosphatases. it also had significant homology (60% identity) with phytase from escherichia coli, while the physical mapping analysis of ...200616369872
emergence of cefotaxime resistance in citrobacter freundii causing necrotizing fasciitis and osteomyelitis.we present a diabetic patient with necrotizing fasciitis caused by citrobacter freundii associated with an injury from the fish fin. two isolates recovered six days after cefotaxime treatment had a cefotaxime minimum inhibitory concentration (mic) of 0.12 and 256 mg/l, respectively, and a cefepime mic of 0.03 and 0.25 mg/l, respectively. the two isolates both possessed bla(cmy-2) gene. the patient responded unsatisfactorily to cefotaxime and cefepime therapy and surgical debridement but recovere ...200616375973
removal of selenate in river and drainage waters by citrobacter braakii enhanced with zero-valent iron.a cost-effective remediation method is needed to remove selenium (se) from se-contaminated water. in this study, a selenate [se(vi)]-reducing bacterium, citrobacter braakii, that is capable of using molasses as a carbon source to reduce se(vi) from natural river and drainage waters was isolated. during an 8-day experiment, 87-97% of the added se(vi) in new river water and white river water, california, was reduced to elemental se [se(0)] or transformed to organic se. in highly saline drainage wa ...200616390192
plasmid-mediated high-level gentamicin resistance among enteric bacteria isolated from pet turtles in louisiana.the sale of small turtles is banned by the food and drug administration from the u.s. market due to concerns about their excretion of salmonella spp. to produce a safe pet for the export market, the louisiana pet turtle industry uses gentamicin sulfate baths (1,000 microg/ml) to eradicate salmonella spp. from turtle eggs. in 1999, we analyzed bacterial samples recovered from turtle farms and found that strains of salmonella enterica subsp. arizonae and other bacteria, such as enterobacter cloaca ...200616391058
distribution of a transposon-like element carrying bla(cmy-2) among salmonella and other enterobacteriaceae.the dissemination of cephamycin resistance in enterobacteriaceae and its correlation with a transposon-like dna element consisting of a specific tnpa-bla(cmy-2)-blc-suge structure were investigated.200616396917
molecular dynamics simulations of class c beta-lactamase from citrobacter freundii: insights into the base catalyst for acylation.herein, we present results from molecular dynamics (md) simulations of the class c beta-lactamase from citrobacter freundii and its michaelis complex with aztreonam. four different configurations of the active site were modeled in aqueous solution, and their relative stability was estimated by means of quantum mechanical energy calculations. for the free enzyme, the energetically most stable configurations present a neutral lys67 residue or an anionic tyr150 side chain. our calculations predict ...200616401074
expression of udp-glucuronosyltransferase isoform mrnas during inflammation and infection in mouse liver and kidney.inflammation or infection down-regulates the activity and expression of cytochrome p450 (p450) enzymes involved in hepatic drug clearance, possibly altering drug effectiveness and leading to toxicity. the regulation of udp-glucuronosyltransferases (ugts) in inflammation and infection is less well characterized. to determine the response of hepatic and renal ugts during inflammation and infection, mice were administered either saline or 1 mg/kg lipopolysaccharide (lps) (16 h), or citrobacter rode ...200616339353
hepatic and renal cytochrome p450 gene regulation during citrobacter rodentium infection in wild-type and toll-like receptor 4 mutant mice.citrobacter rodentium is the rodent equivalent of human enteropathogenic escherichia coli infection. this study investigated regulation of hepatic and renal cytochrome p450 (p450) mrnas, hepatic p450 proteins, cytokines, and acute phase proteins during c. rodentium infection. female c3h/heouj (heou) and c3h/hej (hej) mice [which lack functional toll-like receptor 4 (tlr4)] were infected with c. rodentium by oral gavage and sacrificed 6 days later. hepatic cyp4a10 and 4a14 mrnas were decreased in ...200616339354
microbiological evaluation of bottled non-carbonated ("still") water from domestic brands in greece.the microbiological quality of 1,527 samples of bottled non-carbonated ('still') mineral water, purchased from retail outlets and derived from 10 manufacturing companies in greece, was investigated during the period 1995-2003. applying the membrane filter technique, the aliquots of water samples (250 ml) were analyzed for the presence and enumeration of total coliforms, escherichia coli, enterococcus spp. and pseudomonas aeruginosa. also, aerobic bacteria were counted as heterotrophic plate coun ...200616271413
multiple functions of l0036 in the regulation of the pathogenicity island of enterohaemorrhagic escherichia coli o157:h7.diarrhoeagenic enterohaemorrhagic escherichia coli and enteropathogenic e. coli attach to human intestinal epithelium and efface brush-border microvilli, forming an a/e (attaching and effacing) lesion. these human pathogens are phenotypically similar to the mouse pathogen citrobacter rodentium. genetically, they all have a homologous set of virulent genes involved in the a/e lesion, and these genes are organized on a lee (locus of enterocyte effacement), a pathogenicity island. this island compr ...200616248852
bladder wall abscess following midurethral sling procedure.midurethal sling procedures are gaining popularity as the treatment of choice for stress urinary incontinence. complications that were described include bladder perforation, urinary retention, pelvic hematoma and suprapubic wound infection. sling erosion and pelvic abscess are rare complications of midurethral slings. we report the first case of an abscess formed within the wall of the urinary bladder, 7 months following a midurethral sling procedure.200615965577
citrobacter rodentium infection causes inos-independent intestinal epithelial dysfunction in mice.attaching-effacing bacteria are major causes of infectious diarrhea in humans worldwide. citrobacter rodentium is an attaching-effacing enteric pathogen that causes transmissible murine colonic mucosal hyperplasia. we characterized colonic inflammation and ion transport at 3, 7, 10, 30, and 60 d after infection of c57bl/6 mice with c. rodentium. macroscopic damage score was significantly increased 7 and 10 d after infection. colonic wall thickness was increased at 7, 10, 30, and 60 d. myeloperox ...200617487239
[antagonistic effect of bacteriocinogenic lactobacillus acidophilus on klebsiella pneumoniae, citrobacter freundii and proteus mirabilis cells].data of the ultrastructural cellular changes of conditionally pathogenic enterobacteria, including k. pneumoniae, c. freundii and p. mirabilis cells impacts to bacteriocin-producing l. acidophilus are presented. enterobacteria in response to the bacteriocinogenic effect of lactobacilli are manifestated by expressive destructions of sensitive to pore formation bacteriocin cells. various morphological types of enterobacteria cells with increase of involution, lysing and resting forms are revealed. ...200617297872
[pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy].pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ...200617297889
[overexpression of citrobacter braakii phytase with high specific activity in pichia pastoris].utilization of the phytase with high specific activity is an effective way to improve the fermentation potency of phytase in recombinant host and decrease the production cost. up to now, the phytase appa from citrobacter braakii exhibits the highest specific activity in the all phytases recorded previously. the gene appa encoding phytase was modified according to the bias in codon choice of the high expression gene in pichia pastoris without changing the amino acid sequence and artificially synt ...200617302159
bactericidal activity of normal bovine serum (nbs) directed against some enterobacteriaceae with sialic acid-containing lipopolysaccharides (lps) as a component of cell wall.the sensitivity of bacteria to the bactericidal activity of serum depends on the structure and organization of the bacterial outer membrane. sialic acid has been found in the o-specific region of bacterial lipopolysaccharide (lps) and it plays an essential role in protecting gram-negative bacteria against the bactericidal activity of human and animal serum. the susceptibility of gram-negative bacilli with sialic acid-containing lps to the bactericidal action of normal bovine serum (nbs) was dete ...200617338268
occurrence and characterization of carbapenemase-producing enterobacteriaceae: report from the sentry antimicrobial surveillance program (2000-2004).emergence and dissemination of enterobacteriaceae isolates harboring carbapenemases in various geographic regions represents a significant threat to the management of nosocomial infections. enterobacteriaceae isolates from the sentry antimicrobial surveillance program (2000-2004) demonstrating decreased susceptibility to imipenem and meropenem (minimum inhibitory concentration [mic], > or =2 mg/l) were evaluated for the production of metallo-beta-lactamases and serine carbapenemases using disk a ...200617227206
delayed recurrence of postoperative intra-abdominal abscess: an unusual case and review of the literature.although intra-abdominal abscess is one of the major complications of abdominal surgery, the literature documents only a few cases of recurrence after a long asymptomatic period.200617233573
[clinical results of resection arthroplasty for infected shoulder arthroplasty].infection is a rare complication of shoulder arthroplasty. various therapeutic solutions have been proposed: antibiotics alone, one-stage or two-stage reimplantation, surgical or arthroscopic cleaning without prosthesis removal, scapulohumeral arthrodesis or simple arthroscopic resection. we evaluated the mid-term clinical outcome after resection arthroplasty for the treatment of infected shoulder arthroplasty.200617088753
inactivation of tyrosine phenol-lyase by pictet-spengler reaction and alleviation by t15a mutation on intertwined n-terminal arm.citrobacter freundiil-tyrosine phenol-lyase (tpl) was inactivated by a pictet-spengler reaction between the cofactor and a substrate, 3,4-dihydroxyphenyl-l-alanine (l-dopa), in proportion to an increase in the reaction temperature. random mutagenesis of the tpl gene resulted in the generation of a thr15 to ala mutant (t15a), which exhibited a two-fold improved activity towards l-dopa as the substrate. the thr15 residue was located on the intertwined n-terminal arm of the tpl structure, and compr ...200617094783
identification and characterization of nlei, a new non-lee-encoded effector of enteropathogenic escherichia coli (epec).enteropathogenic escherichia coli (epec), a major gastrointestinal pathogen, causes infantile diarrhea in many developing countries. epec is an attaching and effacing (a/e) pathogen that utilizes a lee-encoded type iii secretion system (ttss) to deliver effector proteins into host cells. these effectors have been identified as potential virulence factors in a/e pathogens including epec, enterohemorrhagic e. coli (ehec) and citrobacter rodentium (cr). we used a proteomics approach to identify a n ...200617097322
flip influences citrobacter koseri macrophage uptake, cytokine expression and brain abscess formation in the neonatal rat.citrobacter koseri causes neonatal meningitis frequently complicated with multiple brain abscesses. during c. koseri central nervous system infection in the neonatal rat model, previous studies have documented many bacteria-filled macrophages within the neonatal rat brain and abscesses. previous studies have also shown that c. koseri is taken up by, survives phagolysosomal fusion and replicates in macrophages in vitro and in vivo. in this study, in order to elucidate genetic and cellular factors ...200617108264
conservative debridement of postoperative meleney's gangrene following a caesarean section.meleney's gangrene is almost exclusively postoperative and is caused by multiple bacteria. this case review describes the conservative management of meleney's gangrene in a young patient following a caesarean section.200617124818
occurrence and diversity of integrons and beta-lactamase genes among ampicillin-resistant isolates from estuarine waters.the aim of the present study was to assess the occurrence and molecular diversity of beta-lactamase genes and integrons among gram-negative ampicillin-resistant bacteria from ria de aveiro. ampicillin-resistant isolates were selected and subjected to genotyping using rep-pcr. representatives from each rep pattern were affiliated with the following taxa by sequencing the 16s rrna gene: aeromonas caviae, a. hydrophila, a. media, a. molluscorum, a. veronii, a. salmonicida, aeromonas sp., pseudomona ...200617125975
transient neutrophilic thrombophagocytosis associated with citrobacter freundii septicemia. 200617149945
enterobacteriaceae in dehydrated powdered infant formula manufactured in indonesia and malaysia.to determine the occurrence of salmonella and shigella in infant formula from southeast asia, 74 packages of dehydrated powdered infant follow-on formula (recommended age, > 4 months) from five different manufacturers, four from indonesia and one from malaysia, were analyzed. none of the 25-g test portions yielded salmonella or shigella. however, further identification of colonies growing on selective media used for salmonella and shigella detection revealed the frequent occurrence of several ot ...200617186672
[using antibiotics in urinary tract infection in a first level of attention health care unit in bogotá, colombia].describing using antibiotics for urinary tract infection in a first level of attention health care unit.200617191601
growth of salmonella enterica in model mixed cultures during a two-step enrichment.growth of salmonella enterica was studied in model mixed cultures with citrobacter freundii or escherichia coli in buffered peptone water (bpw) and in rappaport-vassiliadis medium with soya (rvs) with modified concentrations of mgcl2 and malachite green, and at modified incubation temperatures. selected s. enterica strains were inoculated in bpw (10(0) cfu/ml) together with selected strains of citrobacter freundii (up to 10(8) cfu/ml) or selected strains of escherichia coli (up to 10(8) cfu/ml), ...200617201092
[short communication: prevalence of extended spectrum beta-lactamases in gram negative rods: data of 2001-2004 period].this study was undertaken to determine the prevalence of extended spectrum beta-lactamase (esbl) production by gram negative rods (gnrs) isolated from various clinical specimens (3.240 urine, 336 pus, 277 feces, 83 blood, 38 sputum) sent to our hospital laboratory, between 2001-2004. of isolated bacteria 71.4% were identified as escherichia coli, 11.1% were klebsiella spp, 4.2% were salmonella spp, 3.7% were pseudomonas spp, 3.5% were proteus spp, 2.9% were shigella spp, 2.3% were enterobacter s ...200617205693
microbiologic profile of flexible endoscope disinfection in two brazilian hospitals.[corrected] endoscopes are routinely used in hospitals and clinics of the world and they can be potential sources of cross-infection when the decontamination process is unsuitable200617406750
antimicrobial activity against gram negative bacilli from yaounde central hospital, cameroon.antimicrobial resistance among bacteria pathogens is a world-wide issue. the antimicrobial susceptibility patterns of common pathogenic bacteria are essential to guide empirical and pathogen-specific therapy; unfortunately, these data are scarse in cameroon.200617604512
[cloacal and nasal bacterial flora of lepidochelys olivacea (testudines: cheloniidae) from the north pacific coast of costa rica].cloacal and nasal bacterial flora of lepidochelys olivacea (testudines: cheloniidae) from the north pacific coast of costa rica. the aerobic cloacal and nasal bacterial flora of 45 apparently healthy female olive ridley sea turtles (lepidochelys olivacea) was studied at nancite nesting beach, in santa rosa national park (costa rican north pacific) during july and august 2002. bacterial samples were obtained by inserting sterile swabs directly into the cloaca and the nasal cavities of the turtles ...200618457173
microbial degradation of palm (elaeis guineensis) biodiesel.the kinetics of biodegradation of palm-derived fatty methyl and ethyl esters (elaeis guineensis biodiesel) by a wild-type aerobic bacterial population was measured at 20 degrees c, as the rate of oxygen uptake by a manometric technique. the methyl and ethyl biodiesels were obtained by potassium-hydroxide catalysed trans-esterification of palm oil, respectively. the bacterial flora included the genera bacillus, proteus, pseudomonas, citrobacter and enterobacter. the rate of oxygen uptake for palm ...200618457175
evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae).ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ...200620162076
evaluation of the antimicrobial properties of different parts of citrus aurantifolia (lime fruit) as used locally.we investigated the potency of citrus aurantifolia (lime fruit), against pathogens, in the different forms in which this fruit plant is used locally (juice of the fruit, burnt rind of the fruit commonly known as "epa-ijebu" in the yoruba dialect) and the oil obtained from steam distillation of the fruit. the antimicrobial activity of "epa-ijebu" in different solvents was also compared. the solvents include palm-wine (a local alcoholic drink tapped from palm trees), seaman's schnapps 40% alcoholi ...200620162090
production and characterisation of monoclonal antibodies specific for escherichia coli o157:h7.seven monoclonal antibodies (mabs) specific for escherichia coli o157:h7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising balb/c mice with the strain e. coli o157:h7. these monoclonal antibodies do not cross-react with other bacteria such as salmonella enterica serovar typhimurium, e. coli o14, e. coli jm109, s. enterica serovar enteritidis, s. panama, s. saintpaul, s. derby, s. muenchen, s. bredeney, s. hadar, yersinia enterocolitica, proteus vulgaris, shi ...200620429059
[in vitro activity of arbekacin against clinical isolates of staphylococcus species and gram-negative bacilli].methicillin-resistant staphylococcus aureus (mrsa) and some gram-negative bacilli are very prevalent nosocomial pathogens, commonly causing mixed infections, and are often resistant to multiple drugs. arbekacin is an aminoglycoside used for the treatment of mrsa infections, but is also active against some gram-negative bacilli. the aim of this study was to determine in vitro activity of arbekacin against recent clinical isolates of staphylococci and gram-negative bacilli. materials and200718094591
in vitro antimicrobial activity of crude extracts from plants bryophyllum pinnatum and kalanchoe crenata.extracts from the leaves of bryophyllum pinnatum and kalanchoe crenata were screened for their antimicrobial activities. solvents used included water, methanol, and local solvents such as palmwine, local gin (seaman's schnapps 40% alcoholic drink,) and "omi ekan-ogi" (sour water from 3 days fermented milled maize). leaves were dried and powdered before being soaked in solvents for 3 days. another traditional method of extraction by squeezing raw juice from the leaves was also employed. all extra ...200720161897
[surveillance of antimicrobial resistance among nosocomial gram-negative pathogens from 15 teaching hospitals in china in 2005].to investigate the antimicrobial resistance among the nosocomial gram-negative pathogens from 15 teaching hospitals located in different areas in china in 2005.200718167265
host-mediated inflammation disrupts the intestinal microbiota and promotes the overgrowth of enterobacteriaceae.while the normal microbiota has been implicated as a critical defense against invading pathogens, the impact of enteropathogenic infection and host inflammation on intestinal microbial communities has not been elucidated. using mouse models of citrobacter rodentium, which closely mimics human diarrheal pathogens inducing host intestinal inflammation, and campylobacter jejuni infection, as well as chemically and genetically induced models of intestinal inflammation, we demonstrate that host-media ...200718005726
[fundamental study on the selection of antimicrobial prophylactic agents in abdominal surgery].most infections after abdominal operations are endogenous and occur by disseminating bacteria present in the intestinal tract during operation. the risk of developing surgical site infection after abdominal operations is related to the extent of intestinal contamination during operation and to the density and type of bacteria in the intestinal tract. although antimicrobial prophylactic agents must be active against contaminating bacteria during operation, it should not cover all contaminating ba ...200718018416
in vitro activity of isepamicin and other aminoglycosides against clinical isolates of gram-negative bacteria causing nosocomial bloodstream infections.isepamicin is a newly introduced aminoglycoside in taiwan. since in vitro data for isepamicin against nosocomial gram-negative bloodstream infection from taiwan are limited, we compared the activity of isepamicin, amikacin, gentamicin and tobramycin against nosocomial gram-negative blood isolates.200718087627
microorganisms present in discharging otitis media in a group of patients in kathmandu.discharge from the ear is one of the commonest symptoms of infections of the ear. to study the major strains of bacteria encountered in ear discharges, this study was conducted from july 1999, to june 2001 in the microbiology laboratory of om hospital. a total of 115 patients, of 6-35 years of age clinically diagnosed as discharging otitis media were studied. in 60.9% single organism and in 26.08% two organisms were isolated. results revealed that, staphylococcus aureus (34.3%), klebsiella pneum ...200718092440
antimicrobial resistance patterns of clinical bacterial isolates in southwestern ethiopia.the aim of this study was to assess the pattern of bacterial resistance to antimicrobial agents in a hospital setting in southwest ethiopia.200718326346
[nationwide surveillance of parenteral antibiotics containing meropenem activities against clinically isolated strains in 2006].the antibacterial activity of meropenem (mepm) and other parenteral antibiotics against clinical isolates of 876 strains of gram-positive bacteria, 1764 strains of gram-negative bacteria, and 198 strains of anaerobic bacteria obtained from 30 medical institutions during 2006 was measured. the results were as follows; 1. mepm was more active than the other carbapenem antibiotics tested against gram-negative bacteria, especially against enterobacteriaceae and haemophilus influenzae. mepm was also ...200718447206
[comparison of susceptibilities for fosfomycin determined by various methods in clinical isolates in 2003 -2004].antimicrobial susceptibilities for fosfomycin (fom), cephalexin, cefpodoxime, cefdinir, cefditren, ampicillin, sulbactam/ampicillin, imipenem (ipm), panipenem, meropenem (mepm), biapenem, levofloxacin (lvfx), gatifloxacin, pazufloxacin, prulifloxacin and sulfamethoxazole/trimethoprim were determined by an agar dilution method using mueller-hinton agar (mha) in escherichia coli, klebsiella spp., serratia marcescens, citrobacter spp., enterobacter spp. and proteus mirabilis, which were isolated fr ...200717612258
partial characterisation of the acrab locus in two citrobacter freundii clinical isolates.we studied the mechanisms of resistance to fluoroquinolones in two citrobacter freundii strains (1.44 and 1.38) isolated from the same patient and belonging to the same clone by pulsed-field gel electrophoresis. this study allowed partial characterisation of the acra and acrb genes of this microorganism. as previously reported, the two strains showed the same substitutions in the gyra and parc proteins (thr-83-->ile and asp-87-->tyr in gyra and ser-83-->ile in parc). however, differences were ob ...200717631985
a multiplex polymerase chain reaction method for rapid identification of citrobacter freundii and salmonella species, including salmonella typhi.salmonella enterica is one of the most common enteric pathogens worldwide. conventional methods of isolation of salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. we devised a polymerase chain reaction (pcr) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of salmonella.200717639162
use of biophotonic imaging as a training aid for administration of substances in laboratory rodents.dosing of experimental animals and the removal of blood are two of the most frequent procedures performed in biomedical research using live animals. despite the apparently simple nature of these procedures, they can, if not correctly carried out, have significant effects on the welfare of the animals and the scientific value of the results. there are several methods by which research staff may obtain training in the administration of substances. these include practical demonstrations during teac ...200717640459
pridominant isolates of urinary tract pathogens and their antimicrobial susceptiblity patterns in gondar university teaching hospital, nothwest ethiopia.urinary tract infection (uti) is one of the most common bacterial infections encountered by clinicians in developing countries. although uti seldom leads to complications, it can cause significant morbidity and mortality.200717642159
a generalized transducing phage for the murine pathogen citrobacter rodentium.a virulent phage (phicr1) capable of generalized transduction in citrobacter rodentium was isolated from the environment and characterized. c. rodentium is a natural pathogen of mice, causing transmissible murine colonic hyperplasia. sequencing of its genome has recently been completed and will soon be fully annotated and published. c. rodentium is an important model organism for infections caused by the human pathogens enteropathogenic and enterohaemorrhagic escherichia coli (epec and ehec). ph ...200717768241
[the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)].we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ...200717867488
isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, helixpomatia and cornu aspersum (gastropoda: pulmonata).the intestinal microbiota of the edible snails cornu aspersum fsyn: h. aspersa), and helix pomatia were investigated by culture-based methods, 16s rrna sequence analyses and phenotypic characterisations. the study was carried out on aestivating snails and two populations of h. pomatia were considered. the cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) cfu g -1, but the swedish h. pomatia appeared significantly less colonised, suggesting a higher sensitivity ...200717657348
a novel phytase appa from citrobacter amalonaticus cgmcc 1696: gene cloning and overexpression in pichia pastoris.a novel phytase gene appa, with upstream and downstream sequences from citrobacter amalonaticus cgmcc 1696, was cloned by degenerate polymerase chain reaction (pcr), and thermal asymmetric interlaced (tail) pcr and was overexpressed in pichia pastoris. sequence analysis revealed one open reading frame that consisted of 1311 bp encoding a 436-amino-acid protein, which had a deduced molecular mass of 46.3 kda. the phytase appa belongs to the histidine acid phosphatase family and exhibits the highe ...200717657539
prevalence and diversity of qnr alleles in ampc-producing enterobacter cloacae, enterobacter aerogenes, citrobacter freundii and serratia marcescens: a multicentre study from korea.to investigate the prevalence of qnr determinants, their influence on quinolone susceptibility and their association with other plasmid-mediated genes in ampc-producing enterobacteriaceae without any selection criteria.200717660263
biochemical analysis of tem-134, a new tem-type extended-spectrum beta-lactamase variant produced in a citrobacter koseri clinical isolate from an italian hospital.kinetic characterization of tem-134, a new tem-type extended-spectrum beta-lactamase variant isolated from citrobacter koseri during an italian nationwide survey. tem-134 is a natural derivative of tem-2 with the following substitutions: e104k, r164h and g238s.200717673478
a comparison of ten usepa approved total coliform/e. coli tests.since 2002, the united states environmental protection agency (usepa) has approved ten enzyme-based total coliform and e. coli detection tests for examination of drinking water. these tests include: colilert, colilert-18, colisure, m-coli blue 24, readycult coliforms 100, chromocult, coliscan, e * colite, colitag and mi agar. the utility of the enzyme based test systems is based on both the ability of the test to detect the target organisms at low levels and the ability of the test system to sup ...200717674575
[isolation, identification and characterization of a broad-spectrum azo-reducing citrobacter strain azor-2].a broad-spectrum azo-reducing bacterial strain, azor-2, was isolated from a active-sludge of textile-printing wastewater treatment plant and the strain was identified and characterized. this strain was identified as citrobacter sp. according to its morphological, physiobiochemical characteristics and analysis of its 16s rdna and beta-lactamase gene sequence. studies showed that strain azor-2 was able to reduce various azo dyes with several organic substances and h2 as electron donor and the elec ...200717674757
alternatively activated macrophages in intestinal helminth infection: effects on concurrent bacterial colitis.the distribution of several pathogenic helminth infections coincides geographically with many devastating microbial diseases, including enteric bacterial infections. to dissect the mechanisms by which helminths modulate the host's response to enteric bacteria and bacteria-mediated intestinal inflammation, we have recently established a coinfection model and shown that coinfection with the helminth heligmosomoides polygyrus exacerbates colitis induced by infection with the gram-negative bacterial ...200717878371
antibiotic sensitivity pattern of citrobacter isolated from various clinical specimens in a tertiary care hospital.a total of 709 isolates of citrobacter species were recovered from different clinical specimens at kasturba medical college, mangalore. the antimicrobial susceptibility testing of the isolates was performed using kirby-bauer's method of disk diffusion. all the isolates were susceptible to imipenem (100%) and 100% resistance to ampicillin was recorded. susceptibility to third generation cephalosporins varied between 29 - 43%. the beta-lactamase inhibitor combination used along with beta-lactam an ...200717883179
epidemiology and outcomes of multiple antibiotic-resistant bacterial infection in renal transplantation.mutiresistant bacterial infections are an emerging problem in the nosocomial setting. our objectives were to describe the incidence, outcome, and risk factors for acquisition of multiresistant bacteria among renal transplant recipients.200717889144
cell attachment properties and infectivity of host-adapted and environmentally adapted citrobacter rodentium.citrobacter rodentium belongs to a family of extracellular enteric pathogens that include enterohaemorrhagic and enteropathogenic escherichia coli, which colonises the gastrointestinal mucosa by the attaching and effacing (a/e) mechanism. we previously described the appearance of a 'hyper-infectious' state after passage of c. rodentium through the murine gastrointestinal tract. here we report that host-adapted c. rodentium is able to efficiently adhere and trigger actin polymerisation on culture ...200717890132
dry stress and survival time of enterobacter sakazakii and other enterobacteriaceae in dehydrated powdered infant formula.powdered infant formula is not a sterile product, and opportunistic pathogens could multiply in the reconstituted product, resulting in neonatal infections. in this study, the generation of sublethally injured enterobacteriaceae during desiccation and their persistence in dehydrated powdered infant formula was assessed during a 2.5-year period. the study included 27 strains of enterobacter sakazakii, enterobacter cloacae, salmonella enteritidis, citrobacter koseri, citrobacter freundii, escheric ...200717900090
brain abscess in infants and children: a retrospective study of 107 patients in northeast thailand.to review the clinical manifestation, predisposing factor location of abscess, causative organism, treatment, and outcome of brain abscess.200717926990
bacteriophage-based enrichment coupled to immunochromatographic strip-based detection for the determination of salmonella in meat and poultry.immunochemical-based methods for the detection of salmonella in food can be complicated by the presence of closely related, immunocrossreactive non-salmonella species in the sample that may cause false-positive results. to circumvent this problem, specific bacteriophages against immunocrossreactive, non-salmonella bacteria were used in the sample enrichment step to suppress their growth and improve the performance of an immunochromatographic strip-based detection method for salmonella. cross-rea ...200717969603
composition of intestinal enterobacteriaceae populations of healthy domestic pigs.in this study, the enterobacteriaceae microbiota, including their diversity as well as the distribution of haemolytic and virulence gene-harbouring escherichia coli of 56-day-old healthy piglets, was characterized. both the composition and the diversity of enterobacteriaceae populations varied considerably between individual pigs and intestinal sections. e. coli, enterobacter cloacae, citrobacter freundii and klebsiella pneumoniae dominated the enterobacteriaceae microbiota. however, mucosa-asso ...200717975092
a rapid, small-scale procedure for the structural characterization of lipid a applied to citrobacter and bordetella strains: discovery of a new structural element.endotoxins [lipopolysaccharides (lpss)] are part of the outer cell membrane of gram-negative bacteria. their biological activities are associated mainly with the lipid component (lipid a) and even more specifically with discrete aspects of their fine structure. the need for a rapid and small-scale analysis of lipid a motivated us to develop a procedure that combines direct isolation of lipids a from bacterial cells with sequential release of their ester-linked fatty acids by a mild alkali treatm ...200717703058
antimicrobial resistance among gram-negative bacilli causing infections in intensive care unit patients in the united states between 1993 and 2004.during the 12-year period from 1993 to 2004, antimicrobial susceptibility profiles of 74,394 gram-negative bacillus isolates recovered from intensive care unit (icu) patients in united states hospitals were determined by participating hospitals and collected in a central location. mics for 12 different agents were determined using a standardized broth microdilution method. the 11 organisms most frequently isolated were pseudomonas aeruginosa (22.2%), escherichia coli (18.8%), klebsiella pneumoni ...200717715376
detection of hiv-1 sequences in intestinal bacteria of hiv/aids patients.bacterial dna isolated from the intestinal tract of 11 american and 30 slovak hiv/aids patients were analyzed by colony and dot blot hybridization assay for hiv-1 specific sequences. secondly, pcr using primers specific for the hiv-1 gag, pol and env genes for detection of hiv-1 sequences in these dna were performed.200717984947
multiple antimicrobial resistance in enterobacteriaceae isolates from pristine freshwater.a freshwater enterobacterial population (n = 111) was studied for antimicrobial and mercury resistance patterns, and for its possible association with biotic and abiotic factors in that environment. conventional biochemical tests identified klebsiella sp, morganella sp, serratia sp, escherichia sp, enterobacter sp, edwarsiella sp, proteus sp, citrobacter sp, providencia sp, and kluyvera sp. there was no correlation between antimicrobial resistance patterns of isolates and bacterial genera, but r ...200717985304
a rare case of lethal retroperitoneal abscess caused by citrobacter koseri.retroperitoneal abscesses are very uncommon clinical conditions. the characteristically vague symptomatology of retroperitoneal abscess and the inherent difficulty of identifying retroperitoneal disease by physical examination contributed to these dismal therapeutic outcomes. we present an unusual case of lethal retroperitoneal abscess, caused by citrobacter diversus(koseri), treated with surgical drainage. citrobacter species have rarely been involved in deep tissue infection and there is no re ...200718025858
host-mediated inflammation disrupts the intestinal microbiota and promotes the overgrowth of enterobacteriaceae.while the normal microbiota has been implicated as a critical defense against invading pathogens, the impact of enteropathogenic infection and host inflammation on intestinal microbial communities has not been elucidated. using mouse models of citrobacter rodentium, which closely mimics human diarrheal pathogens inducing host intestinal inflammation, and campylobacter jejuni infection, as well as chemically and genetically induced models of intestinal inflammation, we demonstrate that host-media ...200718030708
what is your diagnosis? emphysematous pyometra with a large amount of gas. 200718045381
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