Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| in vitro evaluation of the use of diluted sodium hypochlorite (bleach) against staphylococcus pseudintermedius, pseudomonas aeruginosa and malassezia pachydermatis. | 2014 | 24589038 | |
| small colony variant of methicillin-resistant staphylococcus pseudintermedius st71 presenting as a sticky phenotype. | we first observed the phenomenon of small colony variants (scvs) in a staphylococcus pseudintermedius sequence type 71 (st71) strain, isolated from a non-pet owner. although we found that small-sized colonies share main features with staphylococcus aureus scvs, they nevertheless show a novel, particular, and sticky phenotype, whose expression was extremely stable, even after subcultivation. | 2014 | 24452163 |
| diversity and evolution of oligopeptide permease systems in staphylococcal species. | several oligopeptide permease (opp) systems have been found in staphylococcal species, including opp1-4, opp3' and the arginine catabolic mobile element (acme)-encoded opp system (acme-opp). they confer upon bacteria the increasing fitness, but their evolutionary histories remain unclear. in this work, we performed a genome-wide identification of opp systems in staphylococcal species. novel opp systems were identified, including the duplicate of opp4 in staphylococcus pseudintermedius and the ac ... | 2014 | 24793159 |
| prevalence and characterization of staphylococcus aureus and staphylococcus pseudintermedius isolated from companion animals and environment in the veterinary teaching hospital in zambia, africa. | the republic of zambia consists of only one veterinary teaching school at the university of zambia (unza) where students and veterinarians are exposed to many bacterial pathogens including staphylococcus aureus (sa) and staphylococcus pseudintermedius (sp). the aim of this study was the characterization and antimicrobial susceptibility profile of eleven sa and 48 sp isolates from the veterinary hospitals' in- and outpatients and the environment. no isolate was resistant to cefoxitin by disk diff ... | 2014 | 24480623 |
| nasal carriage of methicillin-resistant staphylococcus aureus (mrsa) among swiss veterinary health care providers: detection of livestock- and healthcare-associated clones. | we screened a total of 340 veterinarians (including general practitioners, small animal practitioners, large animal practitioners, veterinarians working in different veterinary services or industry), and 29 veterinary assistants for nasal carriage of methicillin-resistant staphylococcus aureus (mrsa) and staphylococcus pseudintermedius (mrsp) at the 2012 swiss veterinary annual meeting. mrsa isolates (n = 14) were detected in 3.8 % (95 % ci 2.1 - 6.3 %) of the participants whereas mrsp was not d ... | 2014 | 24973319 |
| apo, zn2+-bound and mn2+-bound structures reveal ligand-binding properties of sita from the pathogen staphylococcus pseudintermedius. | the gram-positive bacterium staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. s. pseudintermedius also causes life-threatening human infections. furthermore, methicillin-resistant s. pseudintermedius is emerging, resembling the human health threat of methicillin-resistant staphylococcus aureus. therefore it is increasingly important to characterize targets for intervention strategies to counteract s. pseudintermedius infect ... | 2014 | 25311310 |
| first detection of methicillin-resistant staphylococcus aureus st398 and staphylococcus pseudintermedius st68 from hospitalized equines in spain. | eight coagulase-positive staphylococci from equines with different pathologies obtained between 2005 and 2011 were investigated. isolates were characterized by different molecular techniques (spa-, agr-, mlst), and clonal relatedness of strains was investigated by apai and smai pfge. anti-microbial resistance and virulence profiles were determined. six isolates were identified as staphylococcus aureus, and two as staphylococcus pseudintermedius. of these, four isolates were methicillin-resistant ... | 2014 | 23773775 |
| strain typing and antimicrobial susceptibility of methicillin-resistant coagulase-positive staphylococcal species in dogs and people associated with dogs in thailand. | this study was to investigate and to characterize methicillin-resistant coagulase-positive staphylococci (mrcops) harboring in dogs and people associated with dogs in thailand. | 2014 | 24833550 |
| canine superficial bacterial folliculitis: current understanding of its etiology, diagnosis and treatment. | superficial bacterial folliculitis (sbf) is more common in the dog than other mammalian species. until recently, a successful outcome in cases of canine sbf was possible by administering a potentiated amoxicillin, a first generation cephalosporin or a potentiated sulfonamide. unfortunately, this predictable susceptibility has changed, because methicillin resistant staphylococcus pseudintermedius (mrsp) and staphylococcus aureus (mrsa) are becoming more prevalent in canine sbf cases. the increasi ... | 2014 | 24345778 |
| species differentiation within the staphylococcus intermedius group using a refined maldi-tof ms database. | among coagulase-positive staphylococci of animal origin, the members of the staphylococcus intermedius-group (sig: s. intermedius, staphylococcus pseudintermedius and staphylococcus delphini) are important opportunistic pathogens in different animal hosts and occasionally in humans. however, the unambiguous species diagnosis of sig is often challenging. therefore, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) -based sig-identification with bruker mic ... | 2014 | 24807701 |
| amikacin resistance in staphylococcus pseudintermedius isolated from dogs. | staphylococcus pseudintermedius is the most common microorganism isolated from canine pyoderma and postoperative wound infections. the prevalence of methicillin-resistant s. pseudintermedius (mrsp) has increased, and recently, isolates that are resistant not only to methicillin but also to other classes of antibiotic drugs, including aminoglycosides, have become common. a total of 422 s. pseudintermedius isolates collected from 413 dogs were analyzed for amikacin and methicillin resistance using ... | 2014 | 25078911 |
| a novel microfluidic wound model for testing antimicrobial agents against staphylococcus pseudintermedius biofilms. | current methods for testing treatments for veterinary surgical site infections can successfully emulate elements of a chronic wound, but these are time consuming and costly, requiring specialized laboratory equipment and considerable space to house study animals. microfluidic devices however, can be coated with collagen and maintained at basal body temperature, providing a more cost-effective and space-saving model of a chronic wound. our study assesses the applicability of a new microfluidic mo ... | 2014 | 24411017 |
| antibiotic resistance and molecular characterization of ophthalmic staphylococcus pseudintermedius isolates from dogs. | the prevalence, virulence potential, and antibiotic resistance of ophthalmic staphylococcus pseudintermedius (sp) isolated from dogs were examined. sixty-seven staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the staphylococcus intermedius group (sig) nuclease gene (sinuc), exfoliative toxin gene for sig (siet), and antibiotic resistance genes (blaz and meca). pcr-restriction fragment length polymorphism analysis of the pta gene was also ... | 2014 | 24690601 |
| design and application of a loop-mediated isothermal amplification assay for the rapid detection of staphylococcus pseudintermedius. | staphylococcus pseudintermedius is a commensal and opportunistic pathogen of dogs. it is mainly implicated in canine pyoderma, as well as other suppurative conditions of dogs. although bacterial culture is routinely used for clinical diagnosis, molecular methods are required to accurately identify and differentiate s. pseudintermedius from other members of the staphylococcus intermedius group. these methods, owing largely to their cost, are not easy to implement in nonspecialized laboratories or ... | 2014 | 24398904 |
| genetic relatedness, antimicrobial and biocide susceptibility comparative analysis of methicillin-resistant and -susceptible staphylococcus pseudintermedius from portugal. | forty methicillin-resistant and -susceptible staphylococcus pseudintermedius (mrsp and mssp, respectively) from colonization and infection in dogs and cats were characterized for clonality, antimicrobial, and biocide susceptibility. mssp were genetically more diverse than mrsp by multi-locus sequence typing and pulsed-field gel electrophoresis. three different spa types (t06, t02, t05) and two sccmec types (ii-iii and v) were detected in the mrsp isolates. all mrsp and two mssp strains were mult ... | 2014 | 23819785 |
| antibacterial activity of novel cationic peptides against clinical isolates of multi-drug resistant staphylococcus pseudintermedius from infected dogs. | staphylococcus pseudintermedius is a major cause of skin and soft tissue infections in companion animals and has zoonotic potential. additionally, methicillin-resistant s. pseudintermedius (mrsp) has emerged with resistance to virtually all classes of antimicrobials. thus, novel treatment options with new modes of action are required. here, we investigated the antimicrobial activity of six synthetic short peptides against clinical isolates of methicillin-susceptible and mrsp isolated from infect ... | 2014 | 25551573 |
| identification of appropriate reference genes for qpcr studies in staphylococcus pseudintermedius and preliminary assessment of icaa gene expression in biofilm-embedded bacteria. | quantitative pcr is rapidly becoming the standard method for analyzing gene expression in a wide variety of biological samples however it can suffer from significant error if stably expressed reference genes are not identified on which to base the analysis. suitable reference genes for qpcr experiments on staphylococcus pseudintermedius have yet to be identified. | 2014 | 25023435 |
| economic impact of tibial plateau leveling osteotomy surgical site infection in dogs. | to evaluate the economic impact of surgical site infection after tibial plateau leveling osteotomy (tplo) in dogs. | 2014 | 24617450 |
| antimicrobial resistance in methicillin susceptible and methicillin resistant staphylococcus pseudintermedius of canine origin: literature review from 1980 to 2013. | staphylococcus pseudintermedius is a commensal and a common opportunistic pathogen causing mainly infections of the integumentary system in dogs. the recent emergence of multidrug-resistant s. pseudintermedius isolates, in particular methicillin-resistant strains (mrsp) is a threat to small animal health and highlights the need for antimicrobial resistance surveillance to detect trends and potentially perform timeous interventions. we systematically reviewed 202 published articles to investigate ... | 2014 | 24613081 |
| fosfomycin susceptibility of canine methicillin-resistant staphylococcus pseudintermedius isolates. | the effectiveness of fosfomycin was examined across 31 methicillin-resistant staphylococcus pseudintermedius (mrsp) strains by agar dilution. prevalence of the fosfomycin-resistance determinant gene, fosb, was assessed by pcr analysis. results found that 84% of isolates were fosfomycin-susceptible. interestingly, 87% of isolates possessed fosb, indicating no association between this putative staphylococci resistance gene and phenotypic resistance. further evaluation of fosfomycin as a potential ... | 2014 | 24569298 |
| antimicrobial susceptibility of methicillin-resistant staphylococcus pseudintermedius isolated from veterinary clinical cases in the uk. | staphylococcus pseudintermedius is a leading aetiologic agent of pyoderma and other body tissue infections in dogs and cats. in recent years, an increased prevalence of methicillin-resistant s. pseudintermedius (mrsp) has been reported. isolation of mrsp in serious infections poses a major therapeutic challenge as strains are often resistant to all forms of systemic antibiotic used to treat s. pseudintermedius -related infections. this study investigates the occurrence of mrsp from a total of 71 ... | 2014 | 24974679 |
| enhanced adherence of methicillin-resistant staphylococcus pseudintermedius sequence type 71 to canine and human corneocytes. | the recent worldwide spread of methicillin-resistant staphylococcus pseudintermedius (mrsp) in dogs is a reason for concern due to the typical multidrug resistance patterns displayed by some mrsp lineages such as sequence type (st) 71. the objective of this study was to compare the in vitro adherence properties between mrsp and methicillin-susceptible (mssp) strains. four mrsp, including a human and a canine strain belonging to st71 and two canine non-st71 strains, and three genetically unrelate ... | 2014 | 24957656 |
| in vitro synergism of fosfomycin and clarithromycin antimicrobials against methicillin-resistant staphylococcus pseudintermedius. | bacterial biofilms are of tremendous concern for clinicians, as they can compromise the ability of the immune system and antimicrobial therapy to resolve chronic and recurrent infections. novel antimicrobial therapies or combinations targeted against biofilm establishment and growth subsequently represent a promising new option for the treatment of chronic infectious diseases. in this study, we treated bacterial biofilms produced by methicillin-resistant staphylococcus pseudintermedius (mrsp) wi ... | 2014 | 24886369 |
| characterisation of clinical canine meticillin-resistant and meticillin-susceptible staphylococcus pseudintermedius in france. | staphylococcus pseudintermedius is a frequent pathogen in dogs. the emergence of meticillin-resistant s. pseudintermedius (mrsp), which is concomitantly resistant to nearly all veterinary licensed antibiotics used for systemic treatment in dogs, is a major problem for veterinarians. in france, 16.9% (41/243) of the s. pseudintermedius collected in 2010 were mrsp. they mainly belonged to the multiresistant mlst sequence type st71, spa type t02, sccmec type ii-iii (st71-t02-ii-iii) european clone. ... | 2014 | 27873590 |
| prevalence of mupirocin resistance in staphylococcus pseudintermedius. | in the united states, veterinary use of mupirocin is primarily limited to the treatment of canine pyoderma caused by methicillin-resistant staphylococcus pseudintermedius (mrsp). in this study, only 1 of 581 s. pseudintermedius isolates tested was resistant to mupirocin and carried the high-level mupirocin resistance gene, iles2, on a plasmid. | 2014 | 24478408 |
| cluster of infections caused by methicillin-resistant staphylococcus pseudintermedius in humans in a tertiary hospital. | the dog-associated staphylococcus pseudintermedius is a rare pathogen in humans. here we describe a cluster of infections caused by the methicillin-resistant s. pseudintermedius clone st71-j-t02-ii-iii. it involved four elderly patients at a tertiary hospital. three patients had wound infections, and the strain had a tendency to cause bullous skin lesions. | 2014 | 24871217 |
| minocycline pharmacokinetics and pharmacodynamics in dogs: dosage recommendations for treatment of meticillin-resistant staphylococcus pseudintermedius infections. | although minocycline is not licensed for use in dogs, this tetracycline has therapeutic potential against meticillin-resistant staphylococcus pseudintermedius. | 2014 | 24840325 |
| staphylococcus pseudintermedius infection associated with nodular skin lesions and systemic inflammatory response syndrome in a dog. | a 10-year-old pekingese dog with atopic dermatitis was referred due to pyrexia, multiple skin nodules, anorexia, and depression. the dog was diagnosed as having systemic inflammatory response syndrome (sirs) induced by bacterial dermatitis. this case presents diagnosis and treatment of sirs with staphylococcal skin infection in a dog that was immunosuppressed due to long-term use of corticosteroid. | 2014 | 24790236 |
| guidelines for the diagnosis and antimicrobial therapy of canine superficial bacterial folliculitis (antimicrobial guidelines working group of the international society for companion animal infectious diseases). | superficial bacterial folliculitis (sbf) is usually caused by staphylococcus pseudintermedius and routinely treated with systemic antimicrobial agents. infection is a consequence of reduced immunity associated with alterations of the skin barrier and underlying diseases that may be difficult to diagnose and resolve; thus, sbf is frequently recurrent and repeated treatment is necessary. the emergence of multiresistant bacteria, particularly meticillin-resistant s. pseudintermedius (mrsp), has foc ... | 2014 | 24720433 |
| large outbreak caused by methicillin resistant staphylococcus pseudintermedius st71 in a finnish veterinary teaching hospital--from outbreak control to outbreak prevention. | the purpose of this study was to describe a nosocomial outbreak caused by methicillin resistant staphylococcus pseudintermedius (mrsp) st71 sccmec ii-iii in dogs and cats at the veterinary teaching hospital of the university of helsinki in november 2010 - january 2012, and to determine the risk factors for acquiring mrsp. in addition, measures to control the outbreak and current policy for mrsp prevention are presented. | 2014 | 25333798 |
| perioperative administration of antimicrobials during tibial plateau leveling osteotomy. | to evaluate perioperative antimicrobial administration during tibial plateau leveling osteotomy (tplo) in dogs at the ontario veterinary college health sciences centre. | 2014 | 25196889 |
| adverse events associated with chloramphenicol use in dogs: a retrospective study (2007-2013). | chloramphenicol is a broad spectrum antibiotic that has been increasingly utilised since the emergence of methicillin-resistant staphylococcal infections. due to toxicities in humans, use of the drug has been limited. in dogs, gastrointestinal signs are common adverse events described, and bone marrow suppression is possible. the aim of this study was to evaluate the adverse events associated with chloramphenicol in dogs seen by one specialty practice from january 2007 through june 2013. the dat ... | 2014 | 25096589 |
| characterization of meticillin-resistant and meticillin-susceptible isolates of staphylococcus pseudintermedius from cases of canine pyoderma in australia. | meticillin-resistant staphylococcus pseudintermedius (mrsp) has recently emerged as a worldwide cause of canine pyoderma. in this study, we characterized 22 s. pseudintermedius isolates cultured from 19 dogs with pyoderma that attended a veterinary dermatology referral clinic in australia in 2011 and 2012. twelve isolates were identified as mrsp by meca real-time pcr and phenotypic resistance to oxacillin. in addition to β-lactam resistance, mrsp isolates were resistant to erythromycin (91.6 %), ... | 2014 | 24994658 |
| resistance to fluoroquinolones and methicillin in ophthalmic isolates of staphylococcus pseudintermedius from companion animals. | resistance to fluoroquinolones and methicillin was determined for 49 ophthalmic isolates of staphylococcus pseudintermedius from dogs with and without ophthalmic disease. resistance was observed for ciprofloxacin (40.8%), ofloxacin (38.8%), enrofloxacin (38.8%), levofloxacin (34.7%), and moxifloxacin (4.1%). eighteen isolates, 16 of which were resistant to oxacillin, were meca-positive. nine of the 16 oxacillin-resistant meca-positive s. pseudintermedius isolates were resistant to more than one ... | 2014 | 24982521 |
| dam-to-offspring transmission and persistence of staphylococcus pseudintermedius clones within dog families. | staphylococcus pseudintermedius is an opportunistic pathogen that causes mainly skin infections in dogs. although vertical transmission of s. pseudintermedius strains from dam to offspring has been reported, the persistence of the dam's strains in offspring over long periods of time is virtually unknown. | 2014 | 24267321 |
| case-control risk factor study of methicillin-resistant staphylococcus pseudintermedius (mrsp) infection in dogs and cats in germany. | methicillin-resistant staphylococcus pseudintermedius (mrsp) has emerged as a highly drug-resistant small animal veterinary pathogen. although often isolated from outpatients in veterinary clinics, there is concern that mrsp follows a veterinary-hospital-associated epidemiology. this study's objective was to identify risk factors for mrsp infections in dogs and cats in germany. clinical isolates of mrsp cases (n=150) and methicillin-susceptible s. pseudintermedius (mssp) controls (n=133) and the ... | 2014 | 24290489 |
| use of linezolid to treat mrsp bacteremia and discospondylitis in a dog. | a 1.5 yr old male german shepherd dog was evaluated for recurrent intermittent episodes of fever and lethargy. clinicopathologic abnormalities were suggestive of a discospondylitis at the seventh and eighth thoracic vertebrae. blood and urine cultures yielded growth of methicillin-resistant staphylococcus pseudintermedius (mrsp) that was resistant to all commonly used antibiotics. extralabel antibiotic susceptibility testing demonstrated susceptibility of both blood and urine isolates to linezol ... | 2015 | 24216492 |
| new insights in staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain. | staphylococcus pseudintermedius is an opportunistic pathogen recognized as the leading cause of skin, ear, and post-operative bacterial infections in dogs and cats. zoonotic infections have also recently been reported causing endocarditis, infection of surgical wounds, rhinosinusitis, and catheter-related bacteremia. the aim of the present study is to evaluate, for the first time, the pathogenic potential of s. pseudintermedius isolated from a human infection. to this end, strain dsm 25713, whic ... | 2015 | 25994406 |
| lincosamide resistance is less frequent in denmark in staphylococcus pseudintermedius from first-time canine superficial pyoderma compared with skin isolates from clinical samples with unknown clinical background. | antimicrobial resistance may be overestimated in bacterial isolates from clinical samples because veterinarians often submit samples in cases of treatment failure or recurrent cases, which are more frequently associated with resistant strains. | 2015 | 25891140 |
| coagulase-positive staphylococcus: prevalence and antimicrobial resistance. | staphylococcus pseudintermedius is the most prevalent coagulase-positive staphylococcus inhabitant of the skin and mucosa of dogs and cats, causing skin and soft tissue infections in these animals. in this study, coagulase-positive staphylococcus species were isolated from companion animals, veterinary professionals, and objects from a clinical veterinary environment by using two particular culture media, baird-parker rpf agar and chromagar staph aureus. different morphology features of colonies ... | 2015 | 26535454 |
| association between methicillin-resistant staphylococcus pseudintermedius carriage and the development of surgical site infections following tibial plateau leveling osteotomy in dogs. | to evaluate the association between preoperative carriage of methicillin-resistant staphylococcus pseudintermedius (mrsp) and the development of surgical site infections (ssis) following tibial plateau leveling osteotomy (tplo) in dogs. | 2015 | 26421403 |
| negative pressure wound therapy with instillation for body wall reconstruction using an artificial mesh in a dachshund. | a 4-year-old spayed female dachshund was presented in shock, displaying multiple haematomas and puncture wounds along the left abdominal wall and ventral aspect of the abdomen after being attacked by another dog. a defect of the left lateral body wall was palpated. surgery revealed a massive body wall defect and concurrent injury of the intestines. surgical debridement was performed and the injured portion of the jejunum was resected. the abdominal wall was reconstructed using a polypropylene me ... | 2015 | 26412118 |
| a peptide factor secreted by staphylococcus pseudintermedius exhibits properties of both bacteriocins and virulence factors. | staphylococcus pseudintermedius is a common commensal bacterium colonizing the skin and mucosal surfaces of household animals. however, it has recently emerged as a dangerous opportunistic pathogen, comparable to s. aureus for humans. the epidemiological situation is further complicated by the increasing number of methicillin-resistant s. pseudintermedius infections and evidence of gene transmission driving antibiotic resistance between staphylococci colonizing human and zoonotic hosts. in the p ... | 2015 | 26411997 |
| emergence of biofilm-producing staphylococcus pseudintermedius isolated from healthy dogs in south korea. | staphylococcus pseudintermedius is a common bacterial pathogen in dogs with pyoderma, otitis externa, and wound infection. | 2015 | 26404519 |
| pharmacokinetics and pharmacokinetic/pharmacodynamic integration of marbofloxacin after intravenous and intramuscular administration in beagle dogs. | 1.the aim of the present study was to determine the pks of marbofloxacin in beagle dogs after intravenous (i.v.) and intramuscular (i.m.) administration, the ex vivo and in vitro pk/pd indices of marbofloxacin against clinical isolates of staphylococcus pseudintermedius, and the ex vivo auc/mic ratios associated with different levels of antibacterial activity. 2.after i.v. of marbofloxacin (2 mg/kg), the mean ± sem values of auc, t1/2β, vss, and cl were 8.47 ± 3.51 h µg/ml, 8.08 ± 6.25 h, 2.32 ± ... | 2015 | 25470431 |
| genotypic relatedness and characterization of staphylococcus pseudintermedius associated with post-operative surgical infections in dogs. | staphylococcus pseudintermedius is a commensal organism of dogs that can also be implicated in surgical site infections (ssis) in dogs. particularly with the recent emergence and spread of the st71-t02-sccmecii-iii multidrug-resistant s. pseudintermedius clonal lineage (mdrsp), it is important to understand the clonal diversity of s. pseudintermedius in ssis in dogs. the study reported here investigated the genotypic relatedness of 124 s. pseudintermedius isolates from the surgical wounds of 90 ... | 2015 | 26449388 |
| methicillin-resistant staphylococcus pseudintermedius (mrsp) from healthy dogs in norway - occurrence, genotypes and comparison to clinical mrsp. | the aim of the study was to investigate the occurrence of methicillin-resistant staphylococcus pseudintermedius (mrsp) in healthy dogs and further to determine genetic relatedness between carrier isolates and clinical mrsp from dogs in norway. a total of 189 healthy dogs visiting ten veterinary clinics were screened for mrsp during the period february to april 2013. carrier isolates were susceptibility tested with disk diffusion and genotyped using multilocus sequence typing (mlst) and pulsed-fi ... | 2015 | 26423808 |
| direct repeat unit (dru) typing of methicillin-resistant staphylococcus pseudintermedius from dogs and cats. | methicillin-resistant staphylococcus pseudintermedius (mrsp) has emerged in a remarkable manner as an important problem in dogs and cats. however, limited molecular epidemiological information is available. the aims of this study were to apply direct repeat unit (dru) typing in a large collection of well-characterized mrsp isolates and to use dru typing to analyze a collection of previously uncharacterized mrsp isolates. two collections of mrsp isolates from dogs and cats were included in this s ... | 2015 | 26378275 |
| [antimicrobial resistance and molecular epidemiology of staphylococcus pseudintermedius strains isolated from dog clinical samples]. | twenty-eight strains isolated from dog clinical samples identified as staphylococcus pseudintermedius by mass spectrometry (maldi-tof) were studied to assess antimicrobial susceptibility by the diffusion method and clonal relationship by pulsed field gel electrophoresis (pfge). methicillin resistance (3/28 isolates; 10,7%) was evaluated by meca pcr. fifteen strains (53.6%) were resistant to at least one of the antibiotics tested, and eleven of them (39.3%) showed multiple resistance (3 or more a ... | 2015 | 26323373 |
| evaluation of canine-specific minocycline and doxycycline susceptibility breakpoints for meticillin-resistant staphylococcus pseudintermedius isolates from dogs. | using the us clinical and laboratory standards institute (clsi) human tetracycline breakpoints to predict minocycline and doxycycline susceptibility of staphylococcus pseudintermedius (sp) isolates from dogs is not appropriate because they are too high to meet pharmacokinetic/pharmacodynamic data using a standard dose. new breakpoints have been approved for doxycycline and proposed for minocycline. revised breakpoints are four dilutions lower than tetracycline breakpoints, providing a more conse ... | 2015 | 26216025 |
| impact of polymethylmethacrylate additives on methicillin-resistant staphylococcus pseudintermedius biofilm formation in vitro. | to evaluate the impact of gentamicin, silver, or both additives in polymethylmethacrylate (pmma) beads on methicillin-resistant staphylococcus pseudintermedius (mrsp) biofilm formation in vitro. | 2015 | 25909371 |
| phenotypic and genotypic characterization of canine pyoderma isolates of staphylococcus pseudintermedius for biofilm formation. | biofilm-forming ability is increasingly being recognized as an important virulence factor in several staphylococcus species. this study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of s. pseudintermedius, using three phenotypic methods, microtiter plate test (mtp), congo red agar method (cra) and tube adherence test, and the presence and impact of biofilm-associated genes (icaa and icad). the results showed that icaa and icad genes were detected concomitantly i ... | 2015 | 25892615 |
| isolation of coagulase-positive staphylococci from bitches' colostrum and milk and genetic typing of methicillin-resistant staphylococcus pseudintermedius strains. | among the coagulase-positive, potentially pathogenic staphylococci, staphylococcus pseudintermedius has been frequently isolated from bitches' milk. this organism colonizes the mammary gland or causes infection, while s. aureus has been only occasionally reported. the objective of this study was to investigate the occurrence and persistence of coagulase-positive staphylococci in the colostrum and milk of postpartum bitches, either treated or untreated with antimicrobials, and to assess the incid ... | 2015 | 26202520 |
| effectiveness of a combined (4% chlorhexidine digluconate shampoo and solution) protocol in mrs and non-mrs canine superficial pyoderma: a randomized, blinded, antibiotic-controlled study. | there is a lack of studies comparing topical antiseptics to systemic antibiotics in the treatment of canine superficial pyoderma. | 2015 | 26140535 |
| genomic insights into the rapid emergence and evolution of mdr in staphylococcus pseudintermedius. | mdr methicillin-resistant staphylococcus pseudintermedius (mrsp) strains have emerged rapidly as major canine pathogens and present serious treatment issues and concerns to public health due to their, albeit low, zoonotic potential. a further understanding of the genetics of resistance arising from a broadly susceptible background of s. pseudintermedius is needed. | 2015 | 25527273 |
| are staphylococcus intermedius infections in humans cases of mistaken identity? a case series and literature review. | staphylococcus intermedius and staphylococcus pseudintermedius are difficult to distinguish using conventional microbiological methods. molecular diagnostic tools change our understanding of the epidemiology of these 2 organisms. in this study, we present (1) a detailed review of the current literature on molecular diagnostics and (2) a case series in which misidentification was proven in 1 case. we conclude that s pseudintermedius is a more common human pathogen than previously recognized. | 2015 | 26509181 |
| increasing antimicrobial resistance in clinical isolates of staphylococcus intermedius group bacteria and emergence of mrsp in the uk. | frequencies of antimicrobial resistance were determined amongst 14,555 clinical staphylococcus intermedius group (sig) isolates from uk dogs and cats to estimate resistance trends and quantify the occurrence of meticillin-resistant staphylococcus pseudintermedius (mrsp). reports from two diagnostic laboratories (13,313 general submissions, 1242 referral centre only submissions) were analysed retrospectively (2003/2006-2012). mrsp were defined by phenotypic resistance to meticillin and concurrent ... | 2015 | 25376505 |
| characterisation of nasal staphylococcus delphini and staphylococcus pseudintermedius isolates from healthy donkeys in tunisia. | staphylococcus intermedius group (sig) bacteria can colonise the nares of some animals but are also emerging pathogens in humans and animals. | 2015 | 24913693 |
| an evaluation of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of staphylococcus pseudintermedius isolates from canine infections. | it has been proposed, based on taxonomic and molecular studies, that all canine isolates belonging to staphylococcus intermedius group (sig) should be renamed staphylococcus pseudintermedius. however, isolates of sig and other coagulase-positive staphylococci share many phenotypic characteristics, which could lead to misidentification. the accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) for identifying s. pseudintermedius isolates obtained ... | 2015 | 25680922 |
| improved detection of staphylococcus intermedius group in a routine diagnostic laboratory. | the staphylococcus intermedius group (sig) includes zoonotic pathogens traditionally associated with dog bites. we describe a simple scheme for improved detection of sig using routine laboratory methods, report its effect on isolation rates, and use sequencing to confirm that, apart from one atypical sig strain, most isolates are staphylococcus pseudintermedius. | 2015 | 25502532 |
| prevalence and risk factors for isolation of methicillin-resistant staphylococcus in dogs with keratitis. | to determine the prevalence of, and risk factors for, methicillin-resistant staphylococcus (mrs) isolation in dogs with naturally acquired bacterial keratitis. | 2015 | 25130050 |
| prevalence of methicillin-resistant staphylococcus spp. in the conjunctival sac of healthy dogs. | to determine the prevalence of selected coagulase-positive methicillin-resistant staphylococcus aureus (mrs) in the conjunctival sac in a group of healthy dogs and to compare the prevalence of ocular mrs colonization with colonization of typically assessed body sites including the nasal cavity and rectum. | 2015 | 24299189 |
| antibacterial characterization of novel synthetic thiazole compounds against methicillin-resistant staphylococcus pseudintermedius. | staphylococcus pseudintermedius is a commensal organism of companion animals that is a significant source of opportunistic infections in dogs. with the emergence of clinical isolates of s. pseudintermedius (chiefly methicillin-resistant s. pseudintermedius (mrsp)) exhibiting increased resistance to nearly all antibiotic classes, new antimicrobials and therapeutic strategies are urgently needed. thiazole compounds have been previously shown to possess potent antibacterial activity against multidr ... | 2015 | 26086336 |
| anatomical patterns of colonization of pets with staphylococcal species in homes of people with methicillin-resistant staphylococcus aureus (mrsa) skin or soft tissue infection (ssti). | methicillin-resistant strains of staphylococcus aureus (mrsa), staphylococcus pseudintermedius (mrsp), and other pathogenic staphylococci can cause infections in companion animals and humans. identification of colonized animals is fundamental to research and practice needs, but harmonized methods have not yet been established. to establish the optimal anatomic site for the recovery of methicillin-resistant coagulase positive staphylococci (cps), survey data and swabs were collected from 196 pets ... | 2015 | 25623014 |
| a bioengineered nisin derivative to control biofilms of staphylococcus pseudintermedius. | antibiotic resistance and the shortage of novel antimicrobials are among the biggest challenges facing society. one of the major factors contributing to resistance is the use of frontline clinical antibiotics in veterinary practice. in order to properly manage dwindling antibiotic resources, we must identify antimicrobials that are specifically targeted to veterinary applications. nisin is a member of the lantibiotic family of antimicrobial peptides that exhibit potent antibacterial activity aga ... | 2015 | 25789988 |
| susceptibility in vitro of canine methicillin-resistant and -susceptible staphylococcal isolates to fusidic acid, chlorhexidine and miconazole: opportunities for topical therapy of canine superficial pyoderma. | increasing multidrug resistance amongst canine pathogenic staphylococci has renewed interest in topical antibacterial therapy for skin infections in the context of responsible veterinary prescribing. we therefore determined the activity in vitro of three clinically relevant topical agents and synergism between two of them against staphylococcus pseudintermedius and staphylococcus aureus. | 2015 | 25749003 |
| staphylococcus pseudintermedius can be misdiagnosed as staphylococcus aureus in humans with dog bite wounds. | the purpose of this study was to investigate whether s. pseudintermedius is misdiagnosed as s. aureus by clinical laboratories when isolated from humans with dog bite wounds. in addition, we attempted to determine whether s. pseudintermedius isolates related to dog bite wounds share phenotypic and genotypic traits. s. pseudintermedius was identified by pcr targeting the nuc gene. isolates were tested for antibiotic susceptibility using vetmic gp-mo microdilution panels. the occurrence of genes e ... | 2015 | 25532507 |
| oxacillin sensitization of methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus pseudintermedius by antisense peptide nucleic acids in vitro. | antibiotic resistance genes can be targeted by antisense agents, which can reduce their expression and thus restore cellular susceptibility to existing antibiotics. antisense inhibitors can be gene and pathogen specific, or designed to inhibit a group of bacteria having conserved sequences within resistance genes. here, we aimed to develop antisense peptide nucleic acids (pnas) that could be used to effectively restore susceptibility to β-lactams in methicillin-resistant staphylococcus aureus (m ... | 2015 | 26560174 |
| optimization and evaluation of flexicult® vet for detection, identification and antimicrobial susceptibility testing of bacterial uropathogens in small animal veterinary practice. | urinary tract infection (uti) is a common reason for antimicrobial prescription in dogs and cats. the objective of this study was to optimize and evaluate a culture-based point-of-care test for detection, identification and antimicrobial susceptibility testing of bacterial uro-pathogens in veterinary practice. | 2015 | 26503106 |
| in vitro assessment of chloramphenicol and florfenicol as second-line antimicrobial agents in dogs. | the aim of this study was to evaluate the potential of chloramphenicol and florfenicol as second-line antimicrobial agents for treatment of infections caused by methicillin-resistant staphylococcus pseudintermedius (mrsp) and extended-spectrum β-lactamase (esbl)-producing escherichia coli in dogs, through a systematic in vitro assessment of the pharmacodynamic properties of the two drugs. minimum inhibitory concentrations (mic) and phenicol resistance genes were determined for 169 s. pseudinterm ... | 2015 | 25623169 |
| is transmission electron microscopy (tem) a promising approach for qualitative and quantitative investigations of polymyxin b and miconazole interactions with cellular and subcellular structures of staphylococcus pseudintermedius, escherichia coli, pseudomonas aeruginosa and malassezia pachydermatis? | antimicrobial therapy using a combination of polymyxin b and miconazole is effective against the main bacterial pathogens associated with otitis externa in dogs, and a synergistic effect of both drugs has been shown previously. the objective of the present investigation was to visualize ultrastructural changes after exposure of escherichia coli, pseudomonas aeruginosa, staphylococcus pseudintermedius and malassezia pachydermatis to polymyxin b and miconazole by transmission electron microscopic ... | 2015 | 26527257 |
| epidemiology of methicillin resistant staphylococcus pseudintermedius in guide dogs in finland. | methicillin resistant staphylococcus pseudintermedius (mrsp) and staphylococcus aureus (mrsa) are common multi-drug resistant (mdr) bacteria in dogs. in 2012-2013 three dogs of the guide dog school of the finnish federation of the visually impaired were found to be mrsp positive. guide dogs have regular contact with each other during their first year of life and prolonged contact when in training. since dogs are placed in different parts of finland after training, there is a risk for national sp ... | 2015 | 26183814 |
| the distribution of pathogens and their antimicrobial susceptibility patterns among canine surgical wound infections in sweden in relation to different risk factors. | surgical site infection (ssi) is a common nosocomial infection in dogs and a growing concern in veterinary hospitals as an increase in multidrug-resistant pathogens is reported. despite the need for rational and prudent antimicrobial use, few peer-reviewed and published veterinary studies have investigated the pathogenic growth including susceptibility patterns of the isolated pathogens in canine ssis. the first objective of the present study was to estimate the distribution of bacterial pathoge ... | 2015 | 25886937 |
| detection and sequence analysis of accessory gene regulator genes of staphylococcus pseudintermedius isolates. | staphylococcus pseudintermedius (sp) is the major pathogenic species of dogs involved in a wide variety of skin and soft tissue infections. the accessory gene regulator (agr) locus of staphylococcus aureus has been extensively studied, and it influences the expression of many virulence genes. it encodes a two-component signal transduction system that leads to down-regulation of surface proteins and up-regulation of secreted proteins during in vitro growth of s. aureus. the objective of this stud ... | 2015 | 27047173 |
| first identification of methicillin-resistant staphylococcus pseudintermedius strains among coagulase-positive staphylococci isolated from dogs with otitis externa in trinidad, west indies. | otitis externa is a common inflammatory ear disease in dogs caused by a variety of pathogens, and coagulase-positive staphylococci are frequently isolated from such infections. | 2015 | 26715379 |
| fibronectin binding proteins spsd and spsl both support invasion of canine epithelial cells by staphylococcus pseudintermedius. | in this study, we investigated the cell wall-anchored fibronectin-binding proteins spsd and spsl from the canine commensal and pathogen staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (cpek). invasion was examined by the gentamicin protection assay and fluorescence microscopy. an δspsd δspsl mutant of strain ed99 had a dramatically reduced capacity to invade cpek monolayers, while no difference in the invasion level was ... | 2015 | 26238710 |
| antibacterial effect of n-acetylcysteine on common canine otitis externa isolates. | n-acetylcysteine (nac) has the potential to be a useful therapeutic agent for the treatment of otitis externa due to its antimicrobial and mucolytic properties, as well as its ability to disrupt bacterial biofilm. | 2016 | 27122224 |
| the immunopathogenesis of staphylococcal skin infections - a review. | staphylococcus aureus and s. pseudintermedius are the major causes of bacterial skin disease in humans and dogs. these organisms can exist as commensals on the skin, but they can also cause severe or even devastating infections. the immune system has evolved mechanisms to deal with pathogenic microorganisms and has strategies to combat bacteria of this type. what emerges is a delicate "peace" between the opposing sides, but this balance can be disrupted leading to a full blown "war". in the fero ... | 2016 | 27865269 |
| comparison of the in vitro activity of five antimicrobial drugs against staphylococcus pseudintermedius and staphylococcus aureus biofilms. | resistance in canine pathogenic staphylococci is necessitating re-evaluation of the current antimicrobial treatments especially for biofilm-associated infections. long, repeated treatments are often required to control such infections due to the tolerance of bacteria within the biofilm. to comply with the goal of better antibiotic stewardship in veterinary medicine, the efficacies of the available drugs need to be directly assessed on bacterial biofilms. we compared the activities of amoxicillin ... | 2016 | 27531995 |
| pathophysiological mechanisms of staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behavior of s. pseudintermedius. | implicated in more than 60% of bone and joint infections (bjis), staphylococci have a particular tropism for osteoarticular tissue and lead to difficult-to-treat clinical infections. to date, staphylococcus aureus internalization in non-professional phagocytic cells (nppcs) is a well-explored virulence mechanism involved in bji chronicity. conversely, the pathophysiological pathways associated with staphylococcus non-aureus (sna) bjis have scarcely been studied despite their high prevalence. in ... | 2016 | 27462303 |
| trends and molecular mechanisms of antimicrobial resistance in clinical staphylococci isolated from companion animals over a 16 year period. | the objective of this study was to investigate the evolution of resistance to antimicrobials, corresponding mechanisms and molecular characteristics of staphylococcus spp., between 1999 and 2014. | 2016 | 26944924 |
| searching for beta-haemolysin hlb gene in staphylococcus pseudintermedius with species-specific primers. | the paper presents an analysis of 51 staphylococcus pseudintermedius clinically isolated strains from humans and from animals. staphylococcus pseudintermedius strains' ability to produce β-haemolysin was evaluated with phenotypic methods (hot-cold effect, reverse camp test). in order to determine the hlb gene presence (coding for β-haemolysin) in a genomic dna, pcr reactions were conducted with two different pairs of primers: one described in the literature for staphylococcus aureus and recommen ... | 2016 | 27086303 |
| a pilot study comparing in vitro efficacy of topical preparations against veterinary pathogens. | topical antimicrobial agents are important for the management of cutaneous infections. for topical antimicrobial agents, in vitro efficacy data are limited. | 2016 | 27109449 |
| assessment of wound bio-burden and prevalence of multi-drug resistant bacteria during open wound management. | to describe the bacterial bio-burden of open-treated wounds and make comparisons with bite wounds. | 2016 | 27101415 |
| clinical features, cytology and bacterial culture results in dogs with and without cheilitis and comparison of three sampling techniques. | cheilitis is a common presentation in dogs associated with a variety of skin diseases and often complicated by microbial infections. | 2016 | 27019393 |
| antimicrobial susceptibility monitoring of dermatological bacterial pathogens isolated from diseased dogs and cats across europe (compath results). | the compath project is a pan-european programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (mic) determination. here, the susceptibility of major pathogens is reported from antimicrobial nontreated animals with acute clinical signs of skin, wound or ear infections in 2008-2010. | 2016 | 27593360 |
| prevalence and resistance patterns of canine uropathogens in regard to concurrent diseases. | predisposing factors for different types of urinary tract infections (uti) were evaluated and prevalence of causative agents and their resistance were identified. a prospective epidemiologic study (2007 to 2012) included 191 dogs with signs of urinary tract disease. anamnestic data were collected and clinical examination, abdominal ultrasonography, urinalysis and aerobic bacteriologic urine culture were performed in all dogs. other diagnostic procedures were conducted when indicated. uti was mor ... | 2016 | 27529997 |
| comparative in vitro killing of canine strains of staphylococcus pseudintermedius and escherichia coli by cefovecin, cefazolin, doxycycline and pradofloxacin. | bacterial eradication is necessary for clinical cure of infections and antimicrobial agents are important adjunctive therapies for inhibiting the growth of or killing bacteria. pre-existing skin diseases predispose animals to infection by staphylococcus pseudintermedius and, more rarely, by gram-negative bacilli. the property of rapid killing of bacteria may influence drug selection and duration of therapy in the setting of infection. | 2016 | 27307405 |
| sequence-based characterization of tn5801-like genomic islands in tetracycline-resistant staphylococcus pseudintermedius and other gram-positive bacteria from humans and animals. | antibiotic resistance in pathogens is often associated with mobile genetic elements, such as genomic islands (gi) including integrative and conjugative elements (ices). these can transfer resistance genes within and between bacteria from humans and/or animals. the aim of this study was to investigate whether tn5801-like gis carrying the tetracycline resistance gene, tet(m), are common in staphylococcus pseudintermedius from pets, and to do an overall sequences-based characterization of tn5801-li ... | 2016 | 27199912 |
| can ultraviolet light c decrease the environmental burden of antimicrobial-resistant and -sensitive bacteria on textiles? | the environment is important in transmission of bacteria. textiles are difficult and time consuming to clean; ultraviolet light c (uvc) is germicidal and may be an effective disinfection method for textile surfaces. | 2016 | 27620856 |
| systematic review on global epidemiology of methicillin-resistant staphylococcus pseudintermedius: inference of population structure from multilocus sequence typing data. | background and rationale: methicillin-resistant staphylococcus pseudintermedius (mrsp) is a major cause of infections in dogs, also posing a zoonotic risk to humans. this systematic review aimed to determine the global epidemiology of mrsp and provide new insights into the population structure of this important veterinary pathogen. methodology: web of science was searched systematically for articles reporting data on multilocus sequence typing (mlst) of s. pseudintermedius isolates from dogs or ... | 2016 | 27803691 |
| canine influenza virus coinfection with staphylococcus pseudintermedius enhances bacterial colonization, virus load and clinical presentation in mice. | canine influenza virus (civ) and staphylococcus pseudintermedius (sp) are pathogens that cause respiratory disease in dogs. considering bacterial infections following influenza are a leading cause of illness and death, it is of particular meaning to investigate the interaction between these two pathogens. in this study, balb/c mice were used as a mouse model to assess whether inoculation with civ h3n2 followed by s. pseudintermedius 72 h later resulted in exacerbation of disease. disease was cha ... | 2016 | 27259293 |
| evaluation of oxacillin and cefoxitin disk and mic breakpoints for prediction of methicillin resistance in human and veterinary isolates of staphylococcus intermedius group. | staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. human infections with s. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. oxacillin and cefoxitin disk and mic tests were evaluated for the detection of meca- or mecc-mediated methicillin res ... | 2016 | 26607988 |
| linoleic acid salt with ultrapure soft water as an antibacterial combination against dermato-pathogenic staphylococcus spp. | skin colonization of staphylococcus spp. critically affects the severity of dermatitis in humans and animals. we examined different types of fatty acid salts for their antibacterial activity against staphylococcus spp. when used in ultrapure soft water (upsw). we also evaluated their therapeutic effect on a spontaneous canine model of dermatitis. | 2016 | 26606689 |
| in vitro antimicrobial susceptibility of staphylococcus pseudintermedius isolates of human and animal origin. | mic results for 115 staphylococcus intermedius group isolates are presented. of these, 33% were methicillin resistant, among which 51.4% were susceptible to doxycycline, 29.7% to clindamycin, and 21.6% to trimethoprim-sulfamethoxazole. all of the isolates were susceptible to ceftaroline, daptomycin, linezolid, nitrofurantoin, quinupristin-dalfopristin, rifampin, tigecycline, and vancomycin. of all the isolates, 82.6%, 67.8%, and 23.5% were susceptible to ciprofloxacin, erythromycin, and penicill ... | 2016 | 26962087 |
| what's in a name? the impact of accurate staphylococcus pseudintermedius identification on appropriate antimicrobial susceptibility testing. | bacteria in the staphylococcus intermedius group, including staphylococcus pseudintermedius, often encode meca-mediated methicillin resistance. reliable detection of this phenotype for proper treatment and infection control decisions requires that these coagulase-positive staphylococci are accurately identified and specifically that they are not misidentified as s. aureus. as correct species level bacterial identification becomes more commonplace in clinical laboratories, one can expect to see c ... | 2016 | 26763965 |
| evaluation of the impact of methicillin-resistant staphylococcus pseudintermedius biofilm formation on antimicrobial susceptibility. | to compare the minimum inhibitory concentration (mic) of four antimicrobials in planktonic vs. biofilm-associated staphylococcus pseudintermedius. | 2016 | 27500604 |
| the skin microbiome in allergen-induced canine atopic dermatitis. | studies focusing on next-generation sequencing of the bacterial 16s rrna gene have allowed detailed surveys of skin bacterial populations (microbiota) of the skin. | 2016 | 27485242 |
| exploring staphylococcus pseudintermedius: an emerging zoonotic pathogen? | 2016 | 27750440 | |
| quantification and characterization of pleural fluid in healthy dogs with thoracostomy tubes. | objective to quantify and characterize pleural fluid collected from healthy dogs after placement of a thoracostomy tube (tt). animals 8 healthy coonhound-cross dogs (mean ± sd weight, 27.2 ± 1.6 kg). procedures thoracic ct of each dog was performed before placement of a tt and daily thereafter for 7 days. thoracic fluid volume was calculated from ct images. effusion was aspirated when detected; volume was recorded, and cytologic analysis and bacterial culture were performed. results mean ± sd vo ... | 2016 | 27901387 |