Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| an extracellular enzyme from fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin. | among the antimicrobial phytoalexins produced by phaseolus vulgaris (french bean) are the prenylated isoflavonoids kievitone and phaseollidin. two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. the enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electroph ... | 1992 | 1521768 |
| [fusarium solani fusariosis in a leukemic adolescent]. | 1992 | 1530253 | |
| onychomycosis as a possible origin of disseminated fusarium solani infection in a patient with severe aplastic anemia. | 1992 | 1534694 | |
| fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent. | lipases belong to a class of esterases whose activity on triglycerides is greatly enhanced at lipid-water interfaces. this phenomenon, called interfacial activation, has a structural explanation: a hydrophobic lid, which at rest covers the catalytic site, is displaced on substrate or inhibitor binding and probably interacts with the lipid matrix. fusarium solani pisi cutinase belongs to a group of homologous enzymes of relative molecular mass 22-25k (ref. 7) capable of degrading cutin, the insol ... | 1992 | 1560844 |
| lack of activity of amphotericin b in systemic murine fusarial infection. | systemic fusarial infections have emerged as a significant cause of mortality in cancer patients. yet, little is known about the management of these infections. the in vivo antifungal activity of amphotericin b in cf1 mice with disseminated fusarial infections was studied. two pathogenic strains of fusarium solani were used. intraperitoneal administration of amphotericin b in daily doses of 0.5, 1, and 2 mg/kg for less than or equal to 10 days did not prolong survival of treated animals. clearan ... | 1992 | 1583339 |
| a case of contact lens related fusarium solani keratitis. | 1992 | 1606675 | |
| high efficiency transformation of fusarium solani f. sp. cucurbitae race 2 (mating population v). | a cosmid vector, suitable for library construction and dna transformation in filamentous fungi, has been constructed and a reliable and highly efficient peg-mediated dna transformation system for f. solani f. sp. cucurbitae, based on resistance to hygromycin b, has been developed for use with this vector. this transformation system yielded 10(4) transformants per micrograms of dna when using 10(7) protoplasts. factors important in achieving high efficiency included: the maintenance of an osmotic ... | 1992 | 1617735 |
| gas phase transesterification reactions catalyzed by lipolytic enzymes. | porcine pancreatic lipase and fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrates and products are in a gaseous form. in this type of system, enzyme activity was found to depend essentially on the water activity (a(w)) of the enzyme preparation. | 1992 | 18600970 |
| antimicrobial activity of the essential oil of pavonia odorata willd. | the essential oil from the rhizomes of pavonia odorata willd was extracted in an yield of 0.2% by hydrodistillation, and screened for antibacterial and antifungal activity against ten bacteria and thirteen fungi using paper disc agar diffusion technique. the oil was found to inhibit the growth of staphylococcus aureus, diplococcus pheumoniae, escherichia coli and klebsiella sp at 0.55 concentration. the oil was also found to inhibit the growth of keratinophilic fungi trichophyton mentagreophytes ... | 1992 | 22556591 |
| phytotoxic and antimicrobial activity of volatile constituents ofartemisia princeps var.orientalis. | the volatile constituents ofartemisia princeps var.orientalis (wormwood) were investigated for phytotoxic and antimicrobial activities. the germination and radicle elongation of receptor plants were inhibited by volatile substances emitted from wormwood leaf and effects were concentration-dependent. essential oil of the plant extracted by karlsruker's apparatus suppressed seed germination and seedling elongation of the receptor plants at a threshold concentration of 4.8μl/100 ml.escherichia coli ... | 1993 | 24248725 |
| synthesis of fatty acid esters by a recombinant cutinase in reversed micelles. | fusarium solani pisi recombinant cutinase, solubilized in aot/isooctane-reversed micelles, was used to catalyze the esterification of fatty acids with aliphatic alcohols. some relevant parameters for the enzyme activity such as ph, w(o) (water/surfactant molar ratio), temperature, and substrate concentration were optimized. maximal specific activity was obtained for hexanol. the cutinase showed selectivity for short-chain fatty acids. the stability of the microencapsulated cutinase was investiga ... | 1993 | 18613016 |
| interrelationship of heterodera glycines and fusarium solani in sudden death syndrome of soybean. | experiments were established in field microplots to examine the association between heterodera glycines and the blue form of fusarium solani in sudden death syndrome of soybean (sds). foliar disease symptoms occurred on more plants per plot, appeared 3 to 7 days earlier, and were more severe on plants grown in plots infested with f. solani + h. glycines than on those inoculated with f. solani only. yields were suppressed only in treatments that included the nematode. numbers of h. glycines cysts ... | 1993 | 19279791 |
| localized influence of heterodera glycines on sudden death syndrome of soybean. | half-root tests were established to examine the association between heterodera glycines and the blue strain of fusarium solani, the causal agent of sudden death syndrome (sds) of soybean. two independent root systems were established for soybean 'coker 156' and inoculated (half root/half root) with f. solani, h. glycines, both organisms on opposite root halves, both organisms on one root half, or neither one. foliar symptoms were more severe for plants inoculated with both organisms on one root ... | 1993 | 19279825 |
| antifungal activities of crude extracts of mitracarpus villosus (rubiaceae). | extracts of mitracarpus villosus leaves and inflorescences were investigated individually for in vitro antifungal activities by agar-diffusion and tube-dilution techniques. ethanolic extracts produced definite antifungal activities against trichophyton rubrum, microsporum gypseum, candida albicans, aspergillus niger and fusarium solani. the aqueous extracts and the glycerol vehicle control did not inhibit any of the fungi tested. the zones of inhibition produced by the ethanol extracts ranged fr ... | 1993 | 8133654 |
| three cases of fatal infection due to fusarium solani in patients with cancer. | 1993 | 8286647 | |
| [problems found in the isolation of mycelial fungi (fusarium solani) from blood culture (bacter nr-860)]. | f. solani fungemia is unusual. patients at risk are immunosuppressed, have underlying malignancy or severe debilitating diseases. | 1993 | 8305557 |
| non-ulcerative fungal keratitis diagnosed by posterior lamellar biopsy. | we report a case of fusarium solani keratitis which highlights the difficulties often associated with management of fungal corneal infections. this case demonstrates several unusual and interesting features: the occurrence of deep fungal pathology after superficial injury, the difficulties encountered in attempting to isolate and identify the causative organism, and the protracted course taken by an organism often noted to be rapidly destructive. these features necessitated an individual approac ... | 1993 | 8333933 |
| fungal pathogens in etiology of septic shock in neutropenic patients with cancer (short communication). | during the 3 years from 1989 to 1991, we evaluated the etiology of septic shock cases and infection-associated mortality. a total number of 38 patients was included in the study, according to the criteria for septic shock (ss), (intensive care medicine society, 1989). in 1989, p. aeruginosa and enterobacteriaceae among the pathogens prevailed. in 1990 and 1991, s. aureus, enterococci and fungi were most frequent. from 8 patients with ss in 1990, the shock was due to candida albicans in 1 and to ... | 1993 | 8353326 |
| lung injury by furanoterpenoids isolated from fusarium solani infected sweet potato, ipomea batatas. | intraperitoneal administration (1 mg/kg body/wt./day for 21 days) of crude extract of furanoterpenoids, isolated from f. solani damaged i. batatas caused pulmonary oedema in albino rats. the elevated broncho alveolar lavage (bal) angiotensin converting enzyme (ace), lactate dehydrogenase (ldh) and protein levels indicated lung damage. the estimation of pulmonary extracellular surfactant phospholipids showed an alteration in various phospholipid fractions. | 1993 | 8359843 |
| purification and antipathogenic activity of lipid transfer proteins (ltps) from the leaves of arabidopsis and spinach. | two homogeneous proteins active in vitro against the bacterial pathogen clavibacter michiganensis subsp. sepedonicus were obtained from a crude cell-wall preparation from the leaves of columbia wild-type arabidopsis. the n-terminal amino acid sequences of these proteins allowed their identification as lipid transfer proteins (ltp-a1, ltp-a2); the ltp1-a1 sequence was identical to that deduced from a previously described cdna (embl m80566) and ltp-a2 was quite divergent (44% identical positions). ... | 1993 | 8405465 |
| lipid transfer proteins (nsltps) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. | four homogeneous proteins (cw18, cw20, cw21, cw22) were isolated from etiolated barley leaves by extraction of the insoluble pellet from a tris-hcl (ph 7.5) homogenate with 1.5 m licl and fractionation by reverse-phase high-performance liquid chromatography. all 4 proteins inhibited growth of the pathogen clavibacter michiganensis subsp. sepedonicus (ec50s = 1-3 x 10(-7) m) and had closely related n-terminal amino acid sequences. the complete amino acid sequences of proteins cw18 and cw21 were d ... | 1993 | 8420795 |
| catheter-related fusarium solani fungemia and pulmonary infection in a patient with leukemia in remission. | members of the genus fusarium are ubiquitous fungi uncommonly associated with infection. disseminated fusariosis typically occurs in neutropenic hosts and carries a high mortality rate. we present a case of disseminated fusariosis that is unique in terms of its relative lack of symptomatology despite documentation of fungemia and pulmonary nodules. a summary of the literature is also presented. | 1993 | 8448293 |
| fusarium solani cutaneous infection in a neutropenic patient. | a 47-year-old woman with acute myeloblastic leukemia developed disseminated papules and pustules after chemotherapy. a skin biopsy specimen revealed fungal vasculitis and grew fusarium solani. the patient died despite treatment with amphotericin b. the isolate was resistant to amphotericin b, imidazole and 5-fluocytosine. fusarium should be recognized as a potential cause of deep fungal infection in immunocompromised patients. | 1993 | 8453155 |
| engineering cysteine mutants to obtain crystallographic phases with a cutinase from fusarium solani pisi. | cutinases are extracellular enzymes involved in the disruption of cutine, an insoluble polyester which covers the surface of plants. they belong to a class of serine esterases that are able to hydrolyse fatty acid esters and emulsified triglycerides as efficiently as lipases, but without displaying interfacial activation. classical crystallographic methods for obtaining heavy-atom derivatives failed, so the cutinase structure has been solved exclusively by the multiple isomorphous replacement me ... | 1993 | 8475042 |
| pathogenic effects of fusarium sulphureum, fusarium solani var. coeruleum and dry rot affected potatoes on the internal organs of rats. | rats of the wistar race were used in toxicological experiments involving fusarium sulphureum schl., f. solani var. coeruleum (sacc.) booth and potatoes damaged by these fungi. the in vivo and postmortem studies revealed that both fungi and effected tubers had hepatotoxic and nephrotoxic effects on the animals. morphological changes in the internal organs were mainly manifested by disturbances in blood circulation and regressive metamorphosis. these changes intensified proportionally to the dose ... | 1993 | 7504875 |
| induction of a tomato anionic peroxidase gene (tap1) by wounding in transgenic tobacco and activation of tap1/gus and tap2/gus chimeric gene fusions in transgenic tobacco by wounding and pathogen attack. | the anionic peroxidase genes of tomato, tap1 and tap2, are induced by wounding in tomato fruits and by elicitor treatment in cell suspension cultures. these homologous genes code for anionic peroxidases that are postulated to cause polymerization of the phenolic residues into wall polymers in wound-healing and pathogen-infected tissues. an expression construct containing the entire tap1 gene with its 5' and 3' flanking sequences was introduced into tobacco by agrobacterium tumefaciens-mediated g ... | 1993 | 7678769 |
| only specific tobacco (nicotiana tabacum) chitinases and [beta]-1,3-glucanases exhibit antifungal activity. | different isoforms of chitinases and [beta]-1,3-glucanases of tobacco (nicotiana tabacum cv samsun nn) were tested for their antifungal activities. the class i, vacuolar chitinase and [beta]-1,3-glucanase isoforms were the most active against fusarium solani germlings, resulting in lysis of the hyphal tips and in growth inhibition. in additon, we observed that the class i chitinase and [beta]-1,3-glucanase acted synergistically. the class ii isoforms of the two hydrolases exhibited no antifungal ... | 1993 | 12231736 |
| phototoxicity ofcitrus jambhiri to fungi under enhanced uv-b radiation: role of furanocoumarins. | extracts ofcitrus jambhiri foliage exposed to and shielded from uv-b radiation were assayed for phytochemical changes and phototoxicity against four fungal pathogens, two of which (fusarium solani andf. oxysporum) are causative agents of root rots and two of which (penicillium italicum andp. digitatum) are associated with fruit rots. conidial pigment mutants of these four fungal species were assayed to determine whether pigments play a role in protecting fungi against plant photosensitizers. exp ... | 1993 | 24248778 |
| chitosanase from the plant pathogenic fungus, fusarium solani f. sp. phaseoli-purification and some properties. | among 162 strains of the genus fusarium tested, 22 strains, mainly from f. solani and f. splendens, formed halos on chitosan-containing agar medium. chitosanase secreted in the culture by the most effective producer, f. solani f. sp. phaseoli suf386, was further investigated. n-acetylglucosamine (glcnac) used as a carbon source was most effective for production of chitosanase. chitosan, a substrate for chitosanase, inhibited cell growth completely and abolished production of chitosanase when use ... | 1993 | 27314774 |
| genetic and biochemical characterization of nectria haematococca strains with adhesive and adhesion-reduced macroconidia. | a previous study reported the isolation of two mutants (le1 and le2) of the plant pathogenic fungus nectria haematococca (anamorph, fusarium solani f. sp. cucurbitae) with macroconidia with reduced ability to adhere (att) to zucchini fruits and polystyrene. the adhesion-reduced-phenotype in le1 and le2 macroconidia is temperature sensitive and dependent on the concentration of nutrients. classical genetic analysis of progeny derived from le1 identified a mutation in a genetic locus, named att1. ... | 1994 | 16349181 |
| in vitro transcription from the nectria haematococca pda1 promoter in an homologous extract reflects in vivo pisatin-responsive regulation. | the pda1 gene of nectria haematococca mp vi (anamorph: fusarium solani) encodes pisatin demethylase. this enzyme detoxifies the isoflavanoid phytoalexin pisatin produced by the plant on which this fungus is pathogenic. expression of pisatin demethylase activity is induced in a mycelium by pretreatment with pisatin. we have developed homologous in vitro system which accurately initiates transcription from the pda1 promoter. transcription levels in vitro reflect the same pisatin-responsive stimula ... | 1994 | 7750146 |
| sterility and the disinfection potential of indian contact lens solutions. | ocular infection associated with microbial contamination of contact lens care products is a major problem in contact lens wearers. the sterility and the antimicrobial activity of contact lens care systems reflect their suitability for disinfection of contact lenses. these factors remain to be evaluated for the various newer contact lens care products manufactured in india. in this study, 35 bottles of contact lens solutions marketed by different manufacturing units in india were tested for steri ... | 1994 | 7927633 |
| the root epidermis-specific pea gene rh2 is homologous to a pathogenesis-related gene. | two-dimensional gel electrophoresis of pea root and root hair proteins revealed the existence of at least 10 proteins present at elevated levels in root hairs. one of these, named rh2, was isolated and a partial amino acid sequence was determined from two tryptic peptides. using this sequence information oligonucleotides were designed to isolate by pcr an rh2 cdna clone. in situ hybridization studies with this cdna clone showed that rh2 is not only expressed in root hairs, but also in root epide ... | 1994 | 7948884 |
| characterization of the pda1 promoter of nectria haematococca and identification of a region that binds a pisatin-responsive dna binding factor. | isolates of nectria haematococca (anamorph: fusarium solani) are able to detoxify the pea phytoalexin pisatin through expression of pisatin demethylase (pda). this enzyme is a substrate-inducible cytochrome p450 monooxyenase that is encoded by the pda gene family. in the current study, pda1, a highly inducible pda gene, was cloned and the 5' untranslated region was sequenced. the pda mrna levels were measured in pisatin-treated mycelium and found to increase by 20-fold over untreated control. ge ... | 1994 | 8012044 |
| a new class of tobacco chitinases homologous to bacterial exo-chitinases displays antifungal activity. | a novel chitinase gene of tobacco was isolated and characterized by dna sequence analysis of a genomic clone and a cdna clone. comparative sequence analysis of both clones showed an identity of 94%. the proteins encoded by these sequences do not correspond to any of the previously characterized plant chitinases of classes i-iv and are designated as class v chitinases. comparison of the chitinase class v peptide sequence with sequences in the swiss protein databank revealed significant sequence s ... | 1994 | 8012401 |
| fusarium infections in immunocompromised patients: case reports and literature review. | five cases are reported of fusarium infection in patients with aplasia following chemotherapy of leukemia. the clinical signs, diagnosis and course of the infection during treatment are outlined and discussed in conjunction with the characteristics of other cases already reported in the literature. sixty-three cases of fusarium infection have been reported in immunocompromised patients, 44 cases since 1985. these included patients with hematological malignancies (58 cases), especially acute leuk ... | 1994 | 8013489 |
| pseudothionin-st1, a potato peptide active against potato pathogens. | a 5-kda polypeptide, pseudothionin solanum tuberosum 1 (pth-st1), which was active against clavibacter michiganensis subspecies sepedonicus, a bacterial pathogen of potatoes, has been purified from the buffer-insoluble fraction of potato tubers by salt extraction and hpcl. pth-st1 was also active against other potato pathogens tested (pseudomonas solanacearum and fusarium solani). the n-terminal amino acid sequence of this peptide was identical (except for a n/h substitution at position 2) to th ... | 1994 | 8033886 |
| purification and characterization of fus si3596*, a 65 kd allergen of fusarium solani. | a component of fusarium solani (f. solani), identified as the major allergen, fus si3596* was purified to homogeneity from culture filtrate (cf) by means of anion-exchange column chromatography, gel filtration and fplc. the homogeneity of fus si3596* was assessed by ief, page, sds-page (non-reducing), immunoblot and hplc. fus si3596* was isolated as a glycoprotein of mw 65 kd and pi 3.6. the ige elisa-inhibition assay after periodate treatment of the fraction showed a lower ige binding capacity ... | 1994 | 8035781 |
| endogenous endophthalmitis due to fusarium: case report and review. | endogenous fungal endophthalmitis is being increasingly recognized in susceptible individuals. we report a case of endogenous endophthalmitis due to fusarium solani that occurred as the sole clinical manifestation of fungal disease in an immunocompromised host. four previously reported cases of endogenous fusarial endophthalmitis are also reviewed. two of these patients had no underlying disease and presented with isolated endophthalmitis, while two other patients had acute leukemia and presente ... | 1994 | 8038313 |
| cutinase gene disruption in fusarium solani f sp pisi decreases its virulence on pea. | fusarium solani f sp pisi (nectria haematococca) isolate 77-2-3 with one cutinase gene produced 10 to 20% of the cutinase produced by isolate t-8 that has multiple cutinase genes, whereas cutinase gene-disrupted mutant 77-102 of isolate 77-2-3 did not produce cutinase. on the surface of pea stem segments, lesion formation was most frequent and most severe with t-8, less frequent and less severe with 77-2-3, and much less frequent and much milder with the gene-disrupted mutant. microscopic examin ... | 1994 | 8069105 |
| in vitro sensitivity of medically significant fusarium species to various antimycotics. | sixteen isolates belonging to fusarium chlamydosporum (n = 4), fusarium equiseti (n = 1), fusarium moniliforme (n = 2), fusarium oxysporum (n = 3), fusarium proliferatum (n = 1), and fusarium solani (n = 5) were tested against amphotericin b, 5-fluorocytosine, fluconazole, itraconazole, ketoconazole, jai-amphotericin b (water-soluble compound), hamycin and amphotericin b combined with 5-fluorocytosine, using antibiotic medium m3, high-resolution broth (ph 7.1), sabouraud's dextrose, and yeast-ni ... | 1994 | 8082411 |
| studies on fusarium solani. cross-reactivity among fusarium species. | allergenic cross-reactivity among three fusarium species (f. solani, f. equiseti, and f. moniliforme) was determined by crossed immunoelectrophoresis (cie), elisa inhibition, and immunoprint-inhibition analysis. inhibiting immunoprint was used to assess the cross-reactivity of individual allergenic proteins in culture filtrate (cf), separated on sds-page. dose-response inhibition of band 14 kda in immunoprints of f. solani cf by antigens of f. solani cf, f. equiseti cf, and f. moniliforme cf dem ... | 1994 | 8092429 |
| a novel pathogen- and wound-inducible tobacco (nicotiana tabacum) protein with antifungal activity. | a novel pathogen- and wound-inducible antifungal protein of 20 kd was purified from tobacco (nicotiana tabacum) samsun nn leaves inoculated with tobacco mosaic virus (tmv). the protein, designated cbp20, was purified by chitin-affinity chromatography and gel filtration. in vitro assays demonstrated that cbp20 exhibits antifungal activity toward trichoderma viride and fusarium solani by causing lysis of the germ tubes and/or growth inhibition. in addition it was shown that cbp20 acts synergistica ... | 1994 | 8115541 |
| identification of regulatory elements in the cutinase promoter from fusarium solani f. sp. pisi (nectria haematococca). | the cutinase gene from fusarium solani f. sp. pisi (nectria haematococca) is induced upon contact with the plant cuticular polymer, cutin, by the unique hydroxy fatty acid monomers released by cutinase carried by virulent strains of the fungus, and this gene is also catabolite-repressed by glucose. functional elements of the cutinase promoter were studied in vivo by transforming f. solani pisi with fusions of 5'-flanking regions of the cutinase gene and the gene encoding chloramphenicol acetyltr ... | 1994 | 8132657 |
| fusarium solani: immunochemical characterization of allergens. | allergenic components of the fungus fusarium solani were isolated using (nh4)2so4 precipitation and ion-exchange column chromatography. the allergenicity of fractions was studied by enzyme-linked immunosorbent assay and radioallergosorbent test inhibition techniques. proteins of culture filtrate (cf), mycelium (my), and spore (sp) extracts of f. solani were characterized by isoelectrofocusing, sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and ige-specific immunoblotting. cf antigen ... | 1994 | 8199461 |
| in vitro transcription and binding analysis of promoter regulation by a host-specific signal in a phytopathogenic fungus. | the pda1 promoter of the phytopathogennectria haematococca mpvi (anamorph fusarium solani) offers a model for regulation of a fungal virulence gene in response to plant host-specific signals. expression of the pda1 gene, encoding pisatin demethylase, is induced in culture by pisatin, the isoflavanoid phytoalexin of pea. this pisatin induction is suppressed by nutritional factors. we have been studying the mechanism of pisatin induction through in vitro identification of regulatory factors and re ... | 1994 | 7847884 |
| [severe keratomycosis. diagnosis and treatment]. | we reported three consecutive cases of severe fungal keratitis initially undiagnosed by corneal scrapping. corneal biopsy showed fungal elements of paecilomyces lilacinus, fusarium solani, scedosporium apiospermum. most cases of fungal keratitis could be treated successfully if adequate antifungal therapy were started early. early and accurate diagnosis is of major importance. the results of our cases confirm the superiority of corneal biopsy over corneal scrapping. corneal biopsy is a procedure ... | 1994 | 7822693 |
| fungi associated with females and cysts of heterodera glycines in a florida soybean field. | fungal colonization was determined for females and cysts of heterodera glycines on soybean roots or in rhizosphere soil from a florida soybean field. a total of 1,620 females and cysts were examined in 1991, and 1,303 were examined in 1992. more than 35 species of fungi were isolated from females and cysts. the frequency of fungi colonizing white and yellow females was low, but a high frequency of fungi was encountered in brown cysts, which increased with time of exposure of the cysts to the soi ... | 1994 | 19279895 |
| development of heterodera glycines as affected by fusarium solani, the causal agent of sudden death syndrome of soybean. | the effects of the blue form of fusarium solani, the causal agent of sudden death syndrome (sds), on heterodera glycines were examined in the greenhouse. roots of soybean cv. coker 156 were inoculated with either h. glycines alone or f. solani + h. glycines in combination. population levels of h. glycines were reduced 47% in the presence of f. solani. life-stage development of h. glycines increased 3% in 30 days in the presence of f. solani. fusarium solani colonized epidermal and cortical cells ... | 1995 | 19277263 |
| working at controlled water activity in a continuous process: the gas/solid system as a solution. | fusarium solani cutinase and candida cylindracea lipase were used to catalyze a transesterification reaction in a continuous gas/solid bioreactor. in this system, a solid phase composed of a packed enzymatic preparation was continuously percolated with carrier gas which fed substrate and removed reaction products simultaneously. different conditions of immobilization were used and compared to the results obtained with a nonsupported enzyme. the enzymatic activity was found to be highly dependent ... | 1995 | 18623231 |
| disseminated fusarium solani infection. | disseminated fungal infections commonly occur in immunocompromised hosts; candida spp. are the most common. fusarium spp., soil saprophytes once considered pathogenic only in plants, have emerged as serious pathogens in neutropenic patients with malignancies. we describe two patients, one with acute myelogenous leukemia and the other with metastatic breast cancer, in whom disseminated fusarium solani infection developed. both patients had neutropenia and fever when generalized, tender, erythemat ... | 1995 | 7829738 |
| glyceride synthesis catalyzed by cutinase using the monomolecular film technique. | the monomolecular film technique previously used to study the kinetics of lipase hydrolysis was adapted to synthesizing oleoyl glycerides (monoolein, diolein, and triolein). the water subphase was replaced by glycerol, and a film of oleic acid was initially spread on the glycerol surface. in this system a recombinant cutinase from fusarium solani was able to catalyze oleoyl glyceride synthesis. more than 50% of the oleic acid film was acylated after 7 min of reaction. the surface pressure applie ... | 1995 | 7849021 |
| cloning of a novel constitutively expressed pectate lyase gene pelb from fusarium solani f. sp. pisi (nectria haematococca, mating type vi) and characterization of the gene product expressed in pichia pastoris. | since plant-pathogenic fungi must penetrate through pectinaceous layers of the host cell wall, pectin-degrading enzymes are thought to be important for pathogenesis. antibodies prepared against a pectin-inducible pectate lyase (pectate lyase a [pla]) produced by a phytopathogenic fungus, fusarium solani f. sp. pisi (nectria haematococca, mating type vi), was previously found to protect the host from infection. the gene (pela) and its cdna were cloned and sequenced. here we report the isolation o ... | 1995 | 8522511 |
| an exoantigen test for the rapid identification of medically significant fusarium species. | the accurate identification of fusarium species can take 2-3 weeks. preliminary exoantigen studies indicate that a mature culture suspected of being a fusarium species may be immunologically identified 48 h after receipt. exoantigen extracts of 10-day-old slant cultures of fusarium chlamydosporum, fusarium moniliforme (= fusarium verticilloides), fusarium oxysporum, fusarium proliferatum and fusarium solani and partially purified reference homologous and heterologous shake culture extracts (6-we ... | 1995 | 8544080 |
| studies on shared antigenic/allergenic components among fungi. | fungal allergens have been found to be one of the most prevalent aeroallergens in india. knowledge of shared/unique components among different fungi is necessary for proper diagnosis and treatment of patients allergic to fungi. in the present study, crude extracts (ce) of 11 common fungi (alternaria alternata, aspergillus flavus, asp. fumigatus, asp. niger, asp. tamarii, asp. versicolor, cladosporium herbarum, curvularia lunata, mucor hiemalis, penicillium citrinum, and fusarium solani) were cha ... | 1995 | 8607563 |
| fusarium solani dnase is a signal for increasing expression of nonhost disease resistance response genes, hypersensitivity, and pisatin production. | the inoculation of pea endocarp tissue with the bean pathogen fusarium solani f. sp. phaseoli results in a non-host resistance response causing a complete cessation of fungal growth within 6 to 8 h. in addition to previously reported elicitation by chitosan, we now report that components of this response are also induced by a dnase released from this fungus. a single band of protein corresponding with dnase activity elicits phytoalexin production and the accumulation of rna homologous with the p ... | 1995 | 8664496 |
| surface signaling in pathogenesis. | surface signaling plays a major role in fungal infection. topographical features of the plant surface and chemicals on the surface can trigger germination of fungal spores and differentiation of the germ tubes into appressoria. ethylene, the fruit-ripening hormone, triggers germination of conidia, branching of hyphae, and multiple appressoria formation in colletotrichum, thus allowing fungi to time their infection to coincide with ripening of the host. genes uniquely expressed during appressoria ... | 1995 | 7753774 |
| cutinase from fusarium solani pisi hydrolyzing triglyceride analogues. effect of acyl chain length and position in the substrate molecule on activity and enantioselectivity. | triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. the chain length at either position was varied, and both (r)- and (s)-enantiomers of each compound were synthesized. these pseudo triglycerides contain only one hydrolyzable ester bond, and they are ideally suited to studying the influence of the chain length at the 1-, 2-, and 3-position on lipase ... | 1995 | 7756270 |
| structural effect of reversed micelles of aot over a recombinant cutinase from fusarium solani pisi. a steady state fluorescence study. | 1995 | 7785884 | |
| molecular cloning and characterization of a pea chitinase gene expressed in response to wounding, fungal infection and the elicitor chitosan. | the fungicidal class i endochitinases (e.c.3.3.1.14, chitinase) are associated with the biochemical defense of plants against potential pathogens. we isolated and sequenced a genomic clone, dah53, corresponding to a class i basic endochitinase gene in pea, chi1. the predicted amino acid sequence of this chitinase contains a hydrophobic c-terminal domain similar to the vacuole targeting sequences of class i chitinases isolated from other plants. the pea genome contains one gene corresponding to t ... | 1995 | 7787175 |
| construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene. | a gene fusion between the saccharomyces cerevisiae actin gene promoter and the cdna of the fusarium solani f. sp. pisi pela gene has been constructed. this expression cassette has been introduced into the industrial wine yeast strain t73. the resulting recombinant strain is able to secrete active pela enzyme into the culture medium. in preliminary microvinification experiments the wine produced by this pectinolytic strain is indistinguishable from wine produced using the non-transformed strain o ... | 1995 | 7729670 |
| [fusarium solani peritonitis: infrequent complication of peritoneal dialysis]. | 1995 | 7734510 | |
| cloning and expression of cdna encoding a protein that binds a palindromic promoter element essential for induction of fungal cutinase by plant cutin. | previous studies showed that a palindromic sequence located at -159 base pairs is essential for induction of cutinase gene in fusarium solani f. sp. pisi (nectria haematococca mating type vi) by the hydroxy fatty acids from plant cutin and that a 50-kda nuclear protein binds to a promoter that contains this element. screening of a phage lambda gt11 expression library with the concatenated palindromic sequence as the probe identified a cdna encoding a palindrome-binding protein (pbp). nucleotide ... | 1995 | 7744822 |
| triglyceride hydrolysis and stability of a recombinant cutinase from fusarium solani in aot-iso-octane reversed micelles. | a recombinant cutinase from fusarium solani was encapsulated in aot reversed micelles. physicochemical parameters of the system were optimized relative to triolein hydrolysis. kinetic studies of triglyceride hydrolysis showed a decrease in specificity with increase of the acyl chain length. stability of cutinase in the system under study is lower than in aqueous solution and decreases with increase in the water content in the system (w0 = [h2o]/[aot]). the products of triolein hydrolysis had lit ... | 1995 | 7535515 |
| phylogenetic relationships of the soybean sudden death syndrome pathogen fusarium solani f. sp. phaseoli inferred from rdna sequence data and pcr primers for its identification. | phylogenetic relationships of several species within the fusarium solani-complex were investigated using characters from the nuclear ribosomal dna. genetic variation within 24 isolates, including 5 soybean sudden death syndrome (sds) strains, was assessed using rdna sequence data and restriction fragment length polymorphic markers. by these techniques, the causal agent of soybean sds was identified as f. solani f. sp. phaseoli. in separate cladistic analyses, plectosphaerella cucumerina and nect ... | 1995 | 7579615 |
| effect of increasing inoculum sizes of pathogenic filamentous fungi on mics of antifungal agents by broth microdilution method. | inoculum size is a critical variable in development of methods for antifungal susceptibility testing for filamentous fungi. in order to investigate the influence of different inoculum sizes on mics of amphotericin b, 5-fluorocytosine, itraconazole, and miconazole, 32 clinical isolates (8 aspergillus fumigatus, 8 aspergillus flavus, 5 rhizopus arrhizus, 8 pseudallescheria boydii, and 3 fusarium solani isolates) were studied by the broth microdilution method. four inoculum sizes were studied: 1 x ... | 1995 | 7615745 |
| inversion of lipase stereospecificity for fluorogenic alkyldiacyl glycerols. effect of substrate solubilization. | we synthesized enantiomeric 1-o-alkyl-2,3-diacyl-sn-glycerol and 3-o-alkyl-1,2-diacyl-sn-glycerol containing pyrene as a fluorescent reporter and the trinitrophenylamino residue as a fluorescence quencher; both reporter groups were covalently bound to the omega end of the acyl chains at positions sn-2 and sn-3(1), respectively. the fluorescence of the intact substrate molecules was very low. chemical or enzymic release of the fatty acyl chains lead to fluorescence dequenching. the rate of lipoly ... | 1995 | 7628484 |
| construction and heterologous expression of a synthetic copy of the cutinase cdna from fusarium solani pisi. | a copy of the cutinase cdna from fusarium solani pisi was constructed starting from synthetic oligonucleotides. for this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in saccharomyces cerevisiae and aspergillus awamori. | 1995 | 7632392 |
| hyalohyphomycoses due to fusarium spp.--two case reports and review of the literature. | we report a case of a severe fusarium solani keratitis in a 82-year-old patient with a history of surgical trauma. antimycotic therapy and keratoplasty led to markedly improved vision. identification of the fungus was complicated by the fact that the isolate did not produce the typical macroconidia. the second case was a fatal disseminated fusarium verticillioides infection in a 69-year-old patient during neutropenia after chemotherapy of acute myelogenous leukemia. the patient developed pneumon ... | 1995 | 7637684 |
| the fusarium solani gene encoding kievitone hydratase, a secreted enzyme that catalyzes detoxification of a bean phytoalexin. | among the antimicrobial phytoalexins produced by phaseolus vulgaris (french bean) is the prenylated isoflavonoid, kievitone. the bean pathogen, fusarium solani f. sp. phaseoli, secretes a glycoenzyme, kievitone hydratase (ec 4.2.1.95), which catalyzes conversion of kievitone to a less toxic metabolite. among f. solani strains, those that are highly virulent to p. vulgaris also produce kievitone hydratase constitutively, suggesting that the enzyme is a virulence factor. based on the n-terminal am ... | 1995 | 7655061 |
| a case of mycotic keratitis caused by fusarium solani. | a 36-year-old black man, without history of systemic disease or ocular trauma developed a corneal infection in his left eye. he was treated with antibacterial antibiotic and corticosteroids for one month prior to diagnosis. fungal hyphae and chlamydospores were found in a koh preparation of the corneal scrapings, and positive cultures for fusarium solani were obtained in sabouraud dextrose agar. it is emphasized the cautious use of antibiotics and steroids in corneal diseases, and the need of co ... | 1995 | 7481475 |
| cloning of a new pectate lyase gene pelc from fusarium solani f. sp. pisi (nectria haematococca, mating type vi) and characterization of the gene product expressed in pichia pastoris. | antibodies prepared against a pectate lyase (pla) produced by a phytopathogenic fungus fusarium solani f. sp. pisi (nectria haematococca, mating type vi) were previously found to protect the host against infection. the cdna and gene (pela) for pla were cloned and sequenced. a new pectate lyase gene, pelc, was isolated from a genomic library of f. solani pisi with pela cdna as a probe. a 1.3-kb dna fragment containing the pelc gene and its flanking regions was identified and sequenced. the coding ... | 1995 | 7487098 |
| phosphonate analogues of triacylglycerols are potent inhibitors of lipase. | 1,2-dioctylcarbamoylglycero-3-o-p-nitrophenyl alkylphosphonates, with alkyl being methyl or octyl, were synthesised and tested as irreversible inhibitors of cutinase from fusarium solani pisi and staphylococcus hyicus lipase. rapid inactivation of these enzymes occurred with a concomitant release of one mole of p-nitrophenol per mole of enzyme. with both lipases a higher reactivity was observed when the alkyl substituent on the phosphonate is a methyl rather than an octyl chain. both lipases are ... | 1995 | 7492616 |
| recurrence of fusarium solani abscess formation in an otherwise healthy patient. | fusarium spp. are usually considered opportunistic fungi in humans. a case of fusarium solani abscess formation of the foot in an immunocompetent patient in whom recurrence occurred even after intravenous amphotericin b treatment is presented here. | 1995 | 8557391 |
| mixed intranasal infection caused by fusarium solani and a zygomycete in a leukaemic patient. | we report a case of mixed intranasal infection caused by fusarium solani and a zygomycete, with probable sinus and brain involvement. the patient had chronic myelogenous leukaemia and was treated with chemotherapy. diagnosis of the infection was established by direct examination of the intranasal eschar and histopathological study of an excisional biopsy. only f. solani was isolated from both specimens. difficulties in diagnosis and treatment of these infections are discussed. | 1995 | 8559190 |
| tandem mass spectrometry and nuclear magnetic resonance spectroscopy studies of candida bombicola sophorolipids and product formed on hydrolysis by cutinase. | natural mixtures of sophorolipids produced by the yeast candida bombicola have been analyzed by fast atom bombardment (fab)-ms and collision-induced dissociation (cid)-ms. some pure components have been analysed by two-dimensional nmr spectroscopy. the presence of acidic, lactonic, and o-acetylated forms and the position of double bonds in the fatty acid part of these glycolipids can be easily inferred from positive and negative ion fab-mass spectra. details about position of o-acetylation can b ... | 1995 | 8585609 |
| fusarium solani fungal infection of the lateral line canal system in captive scalloped hammerhead sharks (sphyrna lewini) in hawaii. | two of five scalloped hammerhead sharks (sphyrna lewini) captured may 1987 in hawaii (usa) developed granulomatous exudative mycotic dermatitis localized in the lateral line canal system. the lesion initially was noted in the cephalic canals, but over a period of months extended into the lateral canal. fusarium solani and vibrio spp. were isolated from the canal exudate of both sharks. bacterial colonies were not observed in the canal walls or surrounding tissues. fusarium solani infection resul ... | 1995 | 8592393 |
| fusarium polycaprolactone depolymerase is cutinase. | polycaprolactone (pcl), a synthetic polyester, is degraded by a variety of microorganisms, including some phytopathogens. many phytopathogens secrete cutinase, a serine hydrolase that degrades cutin, the structural polymer of the plant cuticle. we compared wild-type strains and a cutinase-negative gene replacement mutant strain of fusarium solani f. sp. pisi (d. j. stahl and w. schäfer, plant cell 4:621-629, 1992) and a wild-type strain of fusarium moniliforme to show that fusarium cutinase is a ... | 1996 | 8593048 |
| identification of elements in the pda1 promoter of nectria haematococca necessary for a high level of transcription in vitro. | expression of the pda1 gene in the ascomycete nectria haematococca mpvi (anamorph: fusarium solani) is induced by exposure of mycelium to pisatin, an isoflavonoid phytoalexin produced by its host plant, garden pea. the pda1 gene encodes a cytochrome p-450 monooxygenase which detoxifies pisatin. regulatory elements controlling transcription from the pda1 promoter were identified using a homologous nectria in vitro transcription system through analysis of 5' deletions, specific oligonucleotide com ... | 1996 | 8569685 |
| disseminated fusariosis. | a 7-year-old boy with t cell acute lymphoblastic leukemia developed disseminated hyalohyphomycosis due to fusarium solani. the clinical features included fever, severe myalgia, documented fungemia with f. solani, an ecthyma gangrenosum-like lesion next to a peripheral venous catheter, and disseminated pustules. severe neutropenia due to chemotherapy was the most relevant risk factor. histopathologic study of the ecthyma gangrenosum-like lesion, as well as pustular lesions, revealed epidermal nec ... | 1996 | 9122067 |
| cutaneous presentation of fusarium solani infection in a bone marrow transplant recipient. | 1996 | 9135599 | |
| oxidative stress in rat liver and lung induced by furanoterpenoids isolated from fusarium solani infected sweet potatoes. | a crude extract containing some toxic furanoterpenoids was isolated from f. solani infected sweet potatoes. chronic administration of the crude extract to male albino rats at a dosage of 1 mg/kg body weight/day for 21 days brought about a sharp increase in the thiobarbituric acid reactive substances and a depression of glutathione levels in the lung and liver homogenates. the antioxidant defense system was affected as evident from a significant fall in the activities of the enzymes, superoxide d ... | 1996 | 8698409 |
| cloning and characterization of mycovirus double-stranded rna from the plant pathogenic fungus, fusarium solani f. sp. robiniae. | a mycovirus (named fusov) from the phytopathogenic fungus, fusarium solani f. sp. robiniae suf704, has two kinds of double-stranded (ds) rna genomes, designated m1 and m2. the cdnas were constructed from fusov genomic dsrnas. the sequences of m1 and m2 cdnas comprised 1645 and 1445bp, respectively. sequence analysis showed that each dsrna had a single long open reading frame (orf) on only one of the strands. m1 orf encodes a 519-amino acid residue polypeptide with a predicted molecular mass of 6 ... | 1996 | 8704307 |
| proteolytic processing of class iv chitinase in the compatible interaction of bean roots with fusarium solani. | three chitinase isoenzymes, pvchie, pvchif, and pvchig (molecular masses 29, 28, 27 kd, respectively), were purified from bean (phaseolus vulgaris l. cv saxa) roots infected with the fungal pathogen fusarium solani f. sp. phaseoli, and their amino acid sequence was partially determined. all sequences from all three isoenzymes exactly matched deduced amino acid sequences of the bean class iv chitinase pvchi4, formerly called pr4. the n terminus of pvchif mapped to the hinge region, and the n term ... | 1996 | 8756497 |
| susceptibility of clinical isolates of fusarium to antifungal drugs. | the inhibitory activities of amphotericin b, fluconazole, itraconazole, miconazole, ketoconazole and terbinafine against nine isolates from clinically apparent infections of fusarium solani, four isolates of fusarium moniliforme and 10 isolates of fusarium oxysporum were determined with an agar diffusion method (neosensitabs) and an agar dilution method. the inhibition zones obtained with antifungal neosensitabs need very careful interpretation. we did not find a good correlation between the aga ... | 1996 | 8786755 |
| amphotericin b lipid complex treatment of a leukemic child with disseminated fusarium solani infection. | we report a case of fusarium solani infection in a 2 1/2-year-old girl with acute lymphoblastic leukemia and severe neutropenia. the infection, initially limited to her sinuses, became disseminated, as evidenced by the development of a characteristic cutaneous lesion on her left leg after a cumulative dose of amphotericin b of 18.3 mg/kg. amphotericin b lipid complex (ablc), 5 mg/kg/day, in conjunction with repeated surgical debridement of the sinuses and correction of neutropenia with granulocy ... | 1996 | 8804544 |
| identification of a novel peld gene expressed uniquely in planta by fusarium solani f. sp. pisi (nectria haematococca, mating type vi) and characterization of its protein product as an endo-pectate lyase. | antibodies prepared against a pectin-inducible pectate lyase (pla) produced by a phytopathogenic fungus fusarium solani f. sp. pisi (nectria haematococca, mating type vi) were previously found to protect the host from infection. the gene (pela) and two of its homologs were cloned and sequenced. here we report the isolation of a new pectate lyase gene, peld, from a genomic library of f. solani pisi. a 1.5-kb dna fragment containing peld and its flanking regions was sequenced. the nucleotide seque ... | 1996 | 8806739 |
| analysis of determinants of binding and transcriptional activation of the pisatin-responsive dna binding factor of nectria haematococca. | pisatin is a fungistatic isoflavonoid produced by garden pea. field isolates of the ascomycete nectria haematococca mating population vi (anamorph: fusarium solani) that are highly virulent on pea have been found to possess the pda1 gene encoding a pisatin detoxifying activity. expression of pda1 is specifically and highly induced by exposure of mycelia to pisatin. a pisatin-responsive dna-binding activity has previously been identified with properties suggestive of a transcriptional regulator o ... | 1996 | 8850087 |
| immobilization of a recombinant cutinase by entrapment and by covalent binding. kinetic and stability studies. | fusarium solani pisi recombinant cutinase, immobilized by entrapment in calcium alginate and by covalent binding on porous silica, was used to catalyze the hydrolysis of tricaprylin. the influence of relevant parameters on the catalytic activity such as ph, temperature, and the substrate concentration were studied. cutinase immobilized by entrapment presented a michaelis-menten kinetics for tricaprylin concentrations up to 200 mm. at higher concentrations of substrate, inhibition was observed. f ... | 1996 | 8933716 |
| kinetic studies of fusarium solani cutinase in a gas/solid system. | 1996 | 8958113 | |
| the effect of pre- and pro-sequences and multicopy integration on heterologous expression of the fusarium solani pisi cutinase gene in aspergillus awamori. | a synthetic derivative of the cutinase cdna of fusarium solani pisi was expressed in aspergillus awamori using the a. awamori endoxylanase ii (exla) promoter and terminator. the influence of the origin of the pre-sequence and the presence of a pro-sequence on the efficiency of extracellular cutinase production was analysed in single-copy transformants containing an expression cassette integrated at the pyrg locus. transformants containing a construct encoding a direct, inframe fusion of the xyla ... | 1996 | 8987467 |
| dynamics of fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants. | in characterizing mutants and covalently inhibited complexes of fusarium solani cutinase, which is a 197-residue lipolytic enzyme, 34 variant structures, crystallizing in 8 different crystal forms, have been determined, mostly at high resolution. taking advantage of this considerable body of information, a structural comparative analysis was carried out to investigate the dynamics of cutinase. surface loops were identified as the major flexible protein regions, particularly those forming the act ... | 1996 | 8990497 |
| contribution of cutinase serine 42 side chain to the stabilization of the oxyanion transition state. | cutinase from the fungus fusarium solani pisi is a lipolytic enzyme able to hydrolyze both aggregated and soluble substrates. it therefore provides a powerful tool for probing the mechanisms underlying lipid hydrolysis. lipolytic enzymes have a catalytic machinery similar to those present in serine proteinases. it is characterized by the triad ser, his, and asp (glu) residues, by an oxyanion binding site that stabilizes the transition state via hydrogen bonds with two main chain amide groups, an ... | 1996 | 8555209 |
| photophysics of the single tryptophan residue in fusarium solani cutinase: evidence for the occurrence of conformational substates with unusual fluorescence behaviour. | the single tryptophan residue, at position 69 in the amino acid sequence, was used as an intrinsic probe to obtain structural and dynamical information on the lipolytic enzyme fusarium solani cutinase. in the enzyme's native state the tryptophan fluorescence is highly quenched. time-resolved experiments reveal that the majority of the excited state species is characterized by an unusually fast decay time of approximately 40 ps, indicating the occurrence of a very efficient nonradiative relaxatio ... | 1996 | 17023358 |
| biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolitic fungi. | the biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and seven fungi species, including imperfect strains and higher level lignolitic species, is compared in a 90-day laboratory experiment using a natural, not-fertilized soil contaminated with 10% crude oil. the natural microbial soil assemblage isolated from an urban forest area was unable to significantly degrade crude oil, whereas pure fungi cultures effectively reduced the residues by 26-35% in 90 days. normal ... | 1996 | 15093496 |
| pathogenicity of fungi to eggs of heterodera glycines. | twenty-one isolates of 18 fungal species were tested on water agar for their pathogenicity to eggs of heterodera glycines. an egg-parasitic index (epi) for each of these fungi was recorded on a scale from 0 to 10, and hatch of nematode eggs was determined after exposure to the fungi on water agar for 3 weeks at 24 c. the epi for verticillium chlamydosporium was 7.6, and the fungus reduced hatch 74%. pyrenochaeta terrestris and two sterile fungi also showed a high epi and reduced hatch 42-73%. ar ... | 1996 | 19277130 |
| fusarium solani invader of the eggs of the insectpanstrongylus geniculatus in a vivarium. | a strain offusarium solani sensu snyder & hansen invaded the eggs of the insectpanstrongylus geniculatus in a vivarium. none of the invaded eggs hatched. to establish experimentally the pathogenicity of thisfusarium species against the eggs ofp. geniculatus, the fungus and the eggs were incubated together under different relative humidities and temperatures. at 64% relative humidity and 26 °c, the fungus grew well colonizing and penetrating all of the chorions.three embryos died and were also co ... | 1996 | 20882454 |
| kinetic studies of fusarium solani pisi cutinase used in a gas/solid system: transesterification and hydrolysis reactions. | fusarium solani cutinase supported onto chromosorb p was used to catalyze transesterification (alcoholysis) and hydrolysis on short volatile alcohols and esters in a continuous gas/solid bioreactor. in this system, a solid phase composed of a packed enzymatic preparation was continuously percolated with carrier gas which fed substrates and removed reaction products simultaneously. a kinetic study was performed under differential operating conditions in order to get initial reaction rates. the ef ... | 1997 | 18636604 |
| predicting the partition coefficients of a recombinant cutinase in polyethylene glycol/phosphate aqueous two-phase systems. | a model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. this model accounts for both charge-independent and electrostatic effects. the determination of nonelectrostatic effects was based on the model of eiteman and gainer for uncharged solutes while the electrostatic contribution was computed using titra, a program that uses a continuum electrostatic model to treat charge interactions in proteins and considers the effect of ph and ionic stren ... | 1997 | 18636640 |
| comparison of soil receptivity to thielaviopsis basicola, aphanomyces euteiches, and fusarium solani f. sp. pisi causing root rot in pea. | abstract soil receptivity as a quantifiable characteristic ranging from conduciveness to suppressiveness to soilborne pea pathogens thielaviopsis basicola and aphanomyces euteiches was determined by analysis of differences in disease response curves obtained by artificial introduction of inoculum into natural field soil samples. several parameters, including maximum root rot severity, the area under the health index curve, scores on the first axis of a principal component analysis (pca) on dose ... | 1997 | 18945109 |