Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| prospective study of the incidence, clinical features, and outcome of symptomatic upper and lower respiratory tract infections by respiratory viruses in adult recipients of hematopoietic stem cell transplants for hematologic malignancies. | respiratory viruses (rvs) are known to be major causes of morbidity and mortality in recipients of hematopoietic stem cell transplants (hscts), but prospective long-term studies are lacking. we prospectively screened all adult hsct recipients (172 allogeneic [allohsct] and 240 autologous [autohsct]) who underwent transplantation during a 4-year period (1999 to 2003) for the development of a first episode of symptomatic upper respiratory tract infections and/or lower respiratory tract infections ... | 2005 | 16182179 |
| [seroprevalence of antibody against human metapneumovirus in beijing]. | to understand the seroprevalence of antibody against the newly identified human metapneumovirus (hmpv) in beijing. | 2005 | 16412351 |
| [detection of human metapneumovirus in children hospitalized for acute lower respiratory infection in santiago, chile]. | human metapneumovirus (hmpv) has recently been described as a new causal agent of acute low respiratory infection (alri) in children. in south america, detection has been reported only in brazil and argentina. | 2005 | 16311698 |
| relevance of human metapneumovirus in exacerbations of copd. | human metapneumovirus (hmpv) is a recently discovered respiratory virus associated with bronchiolitis, pneumonia, croup and exacerbations of asthma. since respiratory viruses are frequently detected in patients with acute exacerbations of copd (ae-copd) it was our aim to investigate the frequency of hmpv detection in a prospective cohort of hospitalized patients with ae-copd compared to patients with stable copd and to smokers without by means of quantitative real-time rt-pcr. | 2005 | 16371156 |
| human metapneumovirus-associated lower respiratory tract infections in korean infants and young children. | to define the role of human metapneumovirus (hmpv) in previously healthy korean children, a retrospective study was done on 166 children with lower respiratory tract infections and on their stored nasal aspirates. the hmpv gene was detected by reverse transcriptase-polymerase chain reaction. twenty-six of 166 individuals tested positive for hmpv. the clinical diagnoses of hmpv infection were pneumonia in 15 children and bronchiolitis in 11 children. | 2005 | 16371878 |
| induction of acute otitis media by human metapneumovirus. | 2005 | 16371887 | |
| human metapneumovirus: a ubiquitous and long-standing respiratory pathogen. | human metapneumovirus (hmpv) is a recently described human pathogen first identified in respiratory specimens of young children suffering from respiratory syndromes ranging from mild to severe. | 2005 | 16378047 |
| human metapneumovirus and community-acquired pneumonia in children. | human metapneumovirus (hmpv) was first recognized in the netherlands in 2001 from nasopharyngeal aspirate samples and was associated with respiratory tract illness in the pediatric population. this was the first report of metapneumovirus infections in community-acquired pneumonia in taiwan. | 2005 | 16382752 |
| understanding the socioeconomic burden of human metapneumovirus in childhood: italian study recommends further research. | 2005 | 16687774 | |
| developments in antiviral drug design, discovery and development in 2004. | this article summarizes key aspects of progress made during 2004 toward the design, discovery and development of antiviral agents for clinical use. important developments in the identification, characterization and clinical utility of inhibitors of human immunodeficiency virus; the hepatitis viruses, hepatitis b, hepatitis c; the herpes family of viruses, herpes simplex viruses 1 and 2, varicella zoster virus, epstein-barr virus and human cytomegalovirus; the respiratory viruses, influenza, resp ... | 2005 | 16535860 |
| pneumonia in older adults. new categories add complexity to diagnosis and care. | the microbiology of community-acquired pneumonia (cap) in older adults, particularly in residents of long-term care facilities, differs from that in the general us population. respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) are important, newly appreciated causes of pneumonia and triggers of comorbid disease exacerbations that often lead to hospitalization of high-risk elderly patients with underlying heart or lung disease. here, dr high examines the diagnostic and treatment s ... | 2005 | 16296261 |
| human metapneumovirus infection in lung transplant recipients: clinical presentation and epidemiology. | in immunocompromised patients, respiratory viruses are likely to lead to lower respiratory tract infections that cause severe morbidity and mortality. we conducted a prospective study from september 2003 to march 2004 to investigate the epidemiology and impact of human metapneumovirus (hmpv) on lung transplant recipients. | 2005 | 16297797 |
| individual contributions of the human metapneumovirus f, g, and sh surface glycoproteins to the induction of neutralizing antibodies and protective immunity. | we evaluated the individual contributions of the three surface glycoproteins of human metapneumovirus (hmpv), namely the fusion f, attachment g, and small hydrophobic sh proteins, to the induction of serum hmpv-binding antibodies, serum hmpv-neutralizing antibodies, and protective immunity. using reverse genetics, each hmpv protein was expressed individually from an added gene in recombinant human parainfluenza virus type 1 (rhpiv1) and used to infect hamsters once or twice by the intranasal rou ... | 2006 | 16300813 |
| prevalence of respiratory viruses, including newly identified viruses, in hospitalised children in austria. | the aim of this epidemiological study was to determine the prevalence of respiratory viruses, including new viruses, in hospitalised children in austria. two hundred fourteen nasopharyngeal samples from hospitalised children were tested for the presence of viruses using cell culture and pcr and/or viral antigen assays. the results revealed a parainfluenza virus 1 (piv1) outbreak that ended right before the onset of the influenza season, with nearly no overlapping, moderate respiratory syncytial ... | 2006 | 17036151 |
| human metapneumovirus infection in hospitalized children with acute respiratory disease in korea. | human metapneumovirus (hmpv) is a recently isolated virus, mostly associated with acute lower respiratory infection in children, of which symptoms are similar to those of respiratory syncytial virus (rsv) infection. the aim of our study was to determine the frequency of hmpv in hospitalized children with acute respiratory tract disease in korea. nasal aspirates from hospitalized children with respiratory infections under 15 yr old between december 2003 and february 2005 were included in the stud ... | 2006 | 17043416 |
| excretion patterns of human metapneumovirus and respiratory syncytial virus among young children. | as respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) cause serious respiratory tract infections, the routes of transmission of these viruses are important to elucidate. we examined the modes of virus shedding and shedding duration of rsv and hmpv in young children. | 2006 | 17052968 |
| human metapneumovirus and respiratory syncytial virus disease in children, yemen. | factors increasing the severity of respiratory infections in developing countries are poorly described. we report factors associated with severe acute respiratory illness in yemeni children (266 infected with respiratory syncytial virus and 66 with human metapneumovirus). age, indoor air pollution, and incomplete vaccinations were risk factors and differed from those in industrialized countries. | 2006 | 17073098 |
| genetic heterogeneity of g and f protein genes from argentinean human metapneumovirus strains. | human metapneumovirus (hmpv) is a newly identified paramixovirus, associated with respiratory illnesses in all age groups. two genetic groups of hmpv have been described. the nucleotide sequences of the g and f genes from 11 argentinean hmpv strains (1998-2003) were determined by rt-pcr and direct sequencing. phylogenetic analysis showed that hmpv strains clustered into two main genetic lineages, a and b. strains clustered into a group were split into two sublineages, a1 and a2. all strains belo ... | 2006 | 16555281 |
| enhanced identification of viral and atypical bacterial pathogens in lower respiratory tract samples with nucleic acid amplification tests. | the advantages of nucleic acid amplification tests (nat) over conventional methods for the detection of pathogens in lower respiratory tract samples have not been established. nat for respiratory pathogens were performed on 439 endotracheal tube (ett) and bronchoalveolar lavage (bal) samples. a potential pathogen was detected in 87 samples. of 22 samples that tested positive by conventional methods, 15 tested positive for the same pathogen by nat, 1 tested positive for a different pathogen, 2 ha ... | 2006 | 16555283 |
| comparison of human metapneumovirus, respiratory syncytial virus and influenza a virus lower respiratory tract infections in hospitalized young children. | we compared the clinical and demographic features of children with lower respiratory tract infection (lri) caused by human metapneumovirus (hmpv), respiratory syncytial virus (rsv) and influenza a virus and sought to determine whether coinfection by hmpv and other respiratory viruses leads to increased disease severity. | 2006 | 16567983 |
| human metapneumovirus infection in young children hospitalized with respiratory tract disease. | human metapneumovirus (hmpv) is a newly recognized pathogen associated with respiratory tract disease (rtd). | 2006 | 16567989 |
| human metapneumovirus, australia, 2001-2004. | we examined 10,025 respiratory samples collected for 4 years (2001-2004) and found a 7.1% average annual incidence of human metapneumovirus. the epidemic peak of infection was late winter to spring, and genotyping showed a change in predominant viral genotype in 3 of the 4 years. | 2006 | 16965711 |
| characterization of human metapneumovirus f protein-promoted membrane fusion: critical roles for proteolytic processing and low ph. | human metapneumovirus (hmpv) is a recently described human pathogen of the pneumovirus subfamily within the paramyxovirus family. hmpv infection is prevalent worldwide and is associated with severe respiratory disease, particularly in infants. the hmpv fusion protein (f) amino acid sequence contains features characteristic of other paramyxovirus f proteins, including a putative cleavage site and potential n-linked glycosylation sites. propagation of hmpv in cell culture requires exogenous trypsi ... | 2006 | 16971452 |
| comparison of multiplex pcr assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. | the performances of four multiplex pcr (m-pcr) were compared to direct immunofluorescence assay (dfa) and huh7 cell culture for the detection of viruses in 263 children admitted to hospital with an acute respiratory illness. one hundred fifty (57.6%) nasal aspirates were found dfa-positive; 188 (72.3%) were found positive by both dfa and huh7 cell culture, and 242 (92%) were pcr-positive. the m-pcr detected 124 viruses which were not found by conventional methods: 68 rhinovirus, 17 human metapne ... | 2006 | 16998894 |
| detection of human coronavirus nl63, human metapneumovirus and respiratory syncytial virus in children with respiratory tract infections in south-west sweden. | two recently detected viruses, human metapneumovirus (hmpv) and coronavirus nl63 (hcov-nl63), have been associated with acute respiratory tract infections, particularly in young children. this study investigated the frequency of hmpv and hcov-nl63 infections in swedish children by screening 221 nasopharyngeal aspirates, collected between november 2003 and may 2005, from 212 children attending the paediatric department of a county hospital in sweden or submitted from local general practitioners. ... | 2006 | 17002608 |
| multi-year study of human metapneumovirus infection at a large us midwestern medical referral center. | because of its recent identification, few multi-year epidemiologic studies of hmpv infection have been reported. | 2006 | 17008122 |
| the cell biology of acute childhood respiratory disease: therapeutic implications. | respiratory syncytial virus (rsv), the recently identified human metapneumovirus (hmpv), and the human parainfluenza viruses (hpivs), cause most cases of childhood croup, bronchiolitis, and pneumonia. influenza virus also causes a significant burden of disease in young children, although its significance in children was not fully recognized until recently. this article discusses pathogens that have been studied for several decades, including rsv and hpivs, and also explores the newly identified ... | 2006 | 17027618 |
| genetic diversity of human metapneumovirus over 4 consecutive years in australia. | the molecular epidemiologic profile of human metapneumovirus (hmpv) infection has likely been skewed toward certain genetic subtypes because of assay-design issues, and no comprehensive studies have been conducted to date. here, reverse-transcription polymerase chain reaction was used to screen 10,319 specimens from patients presenting to hospitals with suspected respiratory tract infections during 2001-2004. after analysis of 727 australian hmpv strains, 640 were assigned to 1 of 4 previously d ... | 2006 | 16703505 |
| human metapneumovirus infection induces long-term pulmonary inflammation associated with airway obstruction and hyperresponsiveness in mice. | human metapneumovirus (hmpv) is a newly described paramyxovirus that is associated with bronchiolitis, pneumonia, and asthma exacerbation. the objective of the present work was to study the duration of pulmonary inflammation and the functional consequences of infection with hmpv by use of a balb/c mouse model. | 2006 | 16703506 |
| differences in clinical severity between genotype a and genotype b human metapneumovirus infection in children. | the clinical spectrum of 69 episodes of metapneumovirus pediatric infection (55 episodes caused by genotype a and 14 episodes caused by genotype b) was analyzed. diagnosis of pneumonia was more common and the illness severity index (determined on the basis of need for hospitalization, oxygen saturation <90%, and intensive care unit stay) was higher for patients with metapneumovirus genotype a infection. | 2006 | 16705567 |
| human metapneumovirus as a causative agent of lower respiratory tract infection in four patients: the first report of human metapneumovirus infection confirmed by rna sequences in taiwan. | human metapneumovirus (hmpv) has been described as a human pathogen associated with respiratory disease in most parts of the world from all age groups. this report presents the first 4 cases of hmpv disease confirmed by the sequences of pcr products in taiwan. | 2006 | 16709547 |
| recovery of avian metapneumovirus subgroup c from cdna: cross-recognition of avian and human metapneumovirus support proteins. | avian metapneumovirus (ampv) causes an acute respiratory disease in turkeys and is associated with "swollen head syndrome" in chickens, contributing to significant economic losses for the u.s. poultry industry. with a long-term goal of developing a better vaccine for controlling ampv in the united states, we established a reverse genetics system to produce infectious ampv of subgroup c entirely from cdna. a cdna clone encoding the entire 14,150-nucleotide genome of ampv subgroup c strain colorad ... | 2006 | 16731918 |
| modification of the trypsin-dependent cleavage activation site of the human metapneumovirus fusion protein to be trypsin independent does not increase replication or spread in rodents or nonhuman primates. | the contribution of cleavage activation of the fusion f protein of human metapneumovirus (hmpv) to replication and pathogenicity in rodents and nonhuman primates was investigated. recombinant hmpvs were generated in which the naturally occurring trypsin-dependent cleavage sequence (r-q-s-r downward arrow) was replaced by each of three sequences whose cleavage in vitro does not depend upon added trypsin. two of these were multibasic sequences derived from avian metapneumovirus type a (r-r-r-r) or ... | 2006 | 16731919 |
| human metapneumovirus infection in a hematopoietic stem cell transplant recipient with relapsed multiple myeloma and rapidly progressing lung cancer. | human metapneumovirus (hmpv) was isolated from a 63-year-old multiple myeloma patient who had undergone hematopoietic stem cell transplantation and who presented with lower respiratory tract infection several weeks prior to the diagnosis of lung cancer. the isolate was phylogenetically and biologically characterized and compared to hmpv prototypes and recent pediatric isolates. remarkably, it belonged to the novel genomic subgroup a2b. | 2006 | 16757646 |
| detection of antibodies against human metapneumovirus by western blot using recombinant nucleocapsid and matrix proteins. | detection of antibodies against individual proteins of human metapneumovirus (hmpv) is important in the analysis of immune responses to hmpv. specific antibodies against nucleocapsid (n) and matrix (m) proteins in 97 serum samples were tested by western blot using recombinant n and m proteins of hmpv expressed in escherichia coli. the results were compared with those of immunofluorescence assays (ifas) based on hmpv-infected llc-mk2 cells, which expressed the whole hmpv proteins. thirty (61.2%) ... | 2006 | 16789025 |
| human metapneumovirus: an important cause of acute respiratory illness. | 2006 | 16802634 | |
| antimicrobial therapy in childhood asthma and wheezing. | there is an increasing number of viral and bacterial pathogens suspected of contributing to asthma pathogenesis in childhood, making it more difficult for the practitioner to make specific therapy decisions. this review discusses the role of viruses, e.g. respiratory syncytial virus, human metapneumovirus, influenza viruses and rhinoviruses, as well as the role of the atypical bacteria chlamydophila pneumoniae and mycoplasma pneumoniae, as contributors to childhood asthma. diagnosis, prevention, ... | 2006 | 16808545 |
| comparison of real-time pcr assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children. | conventional fluorescent-antibody (fa) methods were compared to real-time pcr assays for detection of respiratory syncytial virus (rsv), influenza virus type a (flua), parainfluenza virus types 1, 2, and 3 (piv1, piv2, and piv3), human metapneumovirus (mpv), and adenovirus (adv) in 1,138 specimens from children with respiratory illnesses collected over a 1-year period. at least one virus was detected in 436 (38.3%) specimens by fa and in 608 (53.4%) specimens by pcr (p<0.001). specimen quality w ... | 2006 | 16825353 |
| evidence of human metapneumovirus infection in jordanian children. | 2006 | 16830042 | |
| persistent symptomless human metapneumovirus infection in hematopoietic stem cell transplant recipients. | sequential nasopharyngeal aspirates from patients without respiratory symptoms undergoing hematopoietic stem cell transplantation (hsct) were tested for genomic rna of human metapneumovirus (hmpv). persistent hmpv infection was documented in most of them and confirmed by virus isolation. hmpv infection etiology was also evaluated during the same period in samples from pediatric patients with acute respiratory diseases (ards). sequence analysis of hmpv in hsct recipients documented infection by h ... | 2006 | 16845630 |
| epidemiology of human metapneumovirus. | since the discovery of human metapneumovirus (hmpv) in 2001, the virus has been identified worldwide. hmpv is a common respiratory pathogen, particularly in infants and young children. the virus is associated with both upper and lower respiratory tract infections and may be a trigger for asthma. at least two major genotypes of hmpv circulate during community outbreaks. whether these genotypes represent distinct serotypes remains controversial. the major challenges faced by the medical and scient ... | 2006 | 16847085 |
| detection of human metapneumovirus in respiratory secretions by reverse-transcriptase polymerase chain reaction, indirect immunofluorescence, and virus isolation in human bronchial epithelial cells. | over two winters in newcastle upon tyne, respiratory secretions, negative by immunofluorescence staining for other respiratory viruses, were tested for the presence of human metapneumovirus (hmpv) by rt-pcr. in the second winter, specimens were also tested by immunofluorescence staining with an anti-hmpv polyclonal rabbit antiserum and immunofluorescence positive specimens were inoculated into a line of human bronchiolar cells, 16hbe140. overall, 55 of 549 (10%) specimens tested were positive fo ... | 2006 | 16847967 |
| frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections. | viruses are the major cause of pediatric acute respiratory tract infection (arti) and yet many suspected cases of infection remain uncharacterized. we employed 17 pcr assays and retrospectively screened 315 specimens selected by season from a predominantly pediatric hospital-based population. before the brisbane respiratory virus research study commenced, one or more predominantly viral pathogens had been detected in 15.2% (n = 48) of all specimens. the brisbane study made an additional 206 vira ... | 2006 | 16847968 |
| prevalence and clinical characteristics of human metapneumovirus infections in hospitalized infants in spain. | human metapneumovirus (hmpv), a condition recently described in the netherlands, causes lower respiratory infections, particularly in young children and among the elderly. the objective of this study was to describe the characteristics of hmpv infections in hospitalized infants <2 years of age and to compare them to those of infections caused by respiratory syncytial virus (rsv). a prospective study was conducted on the clinical characteristics of infants admitted to hospital for respiratory inf ... | 2006 | 16850437 |
| absence of human metapneumovirus co-infection in cases of severe respiratory syncytial virus infection. | it has been suggested that co-infection of human metapneumovirus (hmpv) in severe respiratory syncytial (rsv) virus bronchiolitis is very common. to evaluate the epidemiology of hmpv co-infection in children with severe lower respiratory tract infection caused by rsv virus. this was an observational cohort study in which hmpv and rsv viral load was measured by rt-pcr in tracheal specimens from the target population. hmpv could not be detected in any of the 30 mechanically ventilated children wit ... | 2006 | 16850438 |
| respiratory viruses in children younger than five years old with acute respiratory disease from 2001 to 2004 in uberlândia, mg, brazil. | the main viruses involved in acute respiratory diseases among children are: respiratory syncytial virus (rsv), influenzavirus (flu), parainfluenzavirus (piv), adenovirus (adv), human rhinovirus (hrv), and the human metapneumovirus (hmpv). the purpose of the present study was to identify respiratory viruses that affected children younger than five years old in uberlândia, midwestern brazil. nasopharyngeal aspirates from 379 children attended at hospital de clínicas (hc/ufu), from 2001 to 2004, wi ... | 2006 | 16862327 |
| isolation and characterization of monoclonal antibodies which neutralize human metapneumovirus in vitro and in vivo. | human metapneumovirus (hmpv) is a recently described member of the paramyxoviridae family/pneumovirinae subfamily and shares many common features with respiratory syncytial virus (rsv), another member of the same subfamily. hmpv causes respiratory tract illnesses that, similar to human rsv, occur predominantly during the winter months and have symptoms that range from mild to severe cough, bronchiolitis, and pneumonia. like rsv, the hmpv virus can be subdivided into two genetic subgroups, a and ... | 2006 | 16873237 |
| the association of newly identified respiratory viruses with lower respiratory tract infections in korean children, 2000-2005. | this study was performed to evaluate the associations of newly recognized viruses, namely, human metapneumovirus (hmpv), human coronavirus (hcov)-nl63, and human bocavirus (hbov) with lower respiratory tract infections (lrtis) in previously healthy children. | 2006 | 16886150 |
| role of metapneumovirus in viral respiratory infections in young children. | the contribution of human metapneumovirus (hmpv) relative to that of other respiratory viruses as a cause of respiratory infections in children less than 1 year old has been evaluated. from october 2003 to april 2004, nasopharyngeal samples from 211 children less than 1 year old were analyzed to detect respiratory viruses. respiratory syncytial virus (rsv) was the predominant virus isolated (96 children [45.5%]), followed by influenza a virus, parainfluenza virus, adenovirus, cytomegalovirus, an ... | 2006 | 16891486 |
| wheezing in patients with human metapneumovirus infection. | 2006 | 16387621 | |
| the role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience. | the role that human metapneumovirus (hmpv) plays in the etiology of upper respiratory tract infections (uris) in children over a period of many years has not been evaluated previously. | 2006 | 16388486 |
| genetic variability of human metapneumovirus infection: evidence of a shift in viral genotype without a change in illness. | human metapneumovirus (hmpv) was identified in 2001 as a cause of acute respiratory illness, but its characteristics are still being defined. we analyzed 3740 nasopharyngeal-wash specimens obtained during 2002-2004, using assays for common respiratory viruses and real-time polymerase chain reaction for hmpv. we detected hmpv in 5% of all specimens, compared with 28% for other respiratory viruses. nucleotide sequence analysis of hmpv isolates revealed the predominant circulation of hmpv genotype ... | 2006 | 16388487 |
| surveillance of respiratory virus infections in adult hospital admissions using rapid methods. | both influenza and respiratory syncytial virus (rsv) cause epidemics of respiratory illness of variable severity during the winter season. influenza in particular has been blamed for hospital winter bed pressures, although it is thought that rsv may also play a role. human metapneumovirus (hmpv) is a new respiratory virus reported to be important in children; only a limited number of studies are available for adult populations. we aimed to determine initially the burden of virologically confirme ... | 2006 | 16388686 |
| human metapneumovirus infections in hospitalised infants in spain. | human metapneumovirus (hmpv) causes lower respiratory tract infections, particularly in young children and the elderly. | 2006 | 16399780 |
| association of respiratory picornaviruses with acute bronchiolitis in french infants. | human rhinoviruses and enteroviruses (picornaviridae) are suspected to be major viral etiological causes of bronchiolitis in infants. | 2006 | 16406692 |
| serologic evidence of human metapneumovirus circulation in uruguay. | first identified in 2001, the human metapneumovirus (hmpv), is a respiratory tract pathogen that affects young children, elderly, and immunocompromised patients. the present work represents the first serologic study carried out in uruguay. it was performed with the purpose of obtaining serological evidence of hmpv circulation in uruguay and to contribute to the few serologic reports described until now. sixty nine serum samples collected between 1998 and 2001 by vein puncture from patients witho ... | 2006 | 16410956 |
| pneumococcal coinfection with human metapneumovirus. | infection with the newly discovered human metapneumovirus (hmpv) may lead to hospitalization of children with lower respiratory tract infection (lrti), although the pathogenesis thereof remains to be elucidated. | 2006 | 16586360 |
| characterization of human metapneumovirus infections in israel. | respiratory tract infections are a leading cause of morbidity and mortality worldwide. even with the advancement of diagnostic tools, the causative agent of 20 to 30% of upper respiratory tract infections go undiagnosed. recently, a newly identified human respiratory virus, human metapneumovirus (hmpv), was discovered in young children in the netherlands. to study the prevalence of hmpv infections in israeli children, respiratory specimens from 388 hospitalized children less than 5 years of age ... | 2006 | 16597880 |
| human metapneumovirus infections cause similar symptoms and clinical severity as respiratory syncytial virus infections. | human metapneumovirus (hmpv) is a recently discovered pathogen in respiratory tract infection. the published literature suggests milder illness severity in hmpv compared with respiratory syncytial virus (rsv) infection. in two consecutive seasons, 637 nasopharyngeal aspirates from pediatric patients were tested by hmpv polymerase chain reaction, and risk factors and clinical and laboratory items were analyzed. the hmpv patients were compared with hmpv-negative but rsv-positive patients by matche ... | 2006 | 16607540 |
| diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection. | the aetiology of lower respiratory tract infections in young children admitted to the paediatric intensive care unit (picu) is often difficult to establish. however, most infections are believed to be caused by respiratory viruses. a diagnostic study was performed to compare conventional viral tests with the recently developed real-time pcr technique. | 2006 | 16611370 |
| [human coronavirus-nl63 was detected in specimens from children with acute respiratory infection in beijing, china]. | a new human coronavirus, hcov-nl63, was identified recently from two dutch children with acute respiratory infection (ari) by two scientists in the netherlands in 2004. to investigate if this newly discovered virus is associated with acute respiratory infections in pediatric patients in beijing, tests were developed to detect hcov-nl63 gene fragments from throat swab and nasopharyngeal aspirates collected from children in outpatient and inpatient departments with ari in beijing from dec. 2003 to ... | 2006 | 16624059 |
| clinical course of hospitalised children infected with human metapneumovirus and respiratory syncytial virus. | to describe the clinical presentation and outcomes of hospitalised patients infected with human metapneumovirus (hmpv) and human respiratory syncytial virus (hrsv) in a tertiary hospital in cape town, south africa. | 2006 | 16630317 |
| the impact of infection with human metapneumovirus and other respiratory viruses in young infants and children at high risk for severe pulmonary disease. | we conducted a prospective, observational study to characterize the clinical manifestations of respiratory infections caused by human metapneumovirus (hmpv) and other viruses in 194 premature infants and young children with chronic lung disease or congenital heart disease in buenos aires. children had 567 episodes of respiratory illness and were monitored until they were 2 years old or until the completion of the study. hmpv elicited 12 infections (2%) year-round; 30% were of moderate or greater ... | 2006 | 16652283 |
| human metapneumovirus elicits weak ifn-gamma memory responses compared with respiratory syncytial virus. | human metapneumovirus (mpv) is a recently discovered pathogen that causes repeated lower respiratory tract infections beginning in infancy. the prevalence, nature and control of human regulatory responses to mpv are unknown. in this study, we develop and optimize systems to evaluate mpv-driven cytokine responses. using primary culture of human pbmc from previously exposed adults, mpv-stimulated responses were directly compared with those elicited by genetically and clinically similar respiratory ... | 2006 | 16670291 |
| human metapneumovirus and exacerbations of chronic obstructive pulmonary disease. | respiratory viruses are a common trigger for exacerbations of chronic obstructive pulmonary disease (copd). human metapneumovirus (hmpv) is a paramyxovirus associated with respiratory tract infections and wheezing. our aim was to determine whether hmpv was associated with exacerbations of copd. | 2006 | 16412516 |
| impact of human metapneumovirus and human cytomegalovirus versus other respiratory viruses on the lower respiratory tract infections of lung transplant recipients. | viral respiratory tract infections in lung transplant recipients may be severe. during three consecutive winter-spring seasons, 49 symptomatic lung transplant recipients with suspected respiratory viral infection, and 26 asymptomatic patients were investigated for presence of respiratory viruses either in 56 nasopharyngeal aspirate or 72 bronchoalveolar lavage samples taken at different times after transplantation. on the whole, 1 asymptomatic (3.4%) and 28 symptomatic (57.1%) patients were posi ... | 2006 | 16419110 |
| outbreak of human metapneumovirus infection in elderly inpatients in japan. | 2006 | 16420227 | |
| viral pneumonia in older adults. | viruses account for a substantial portion of respiratory illnesses, including pneumonia, in the elderly population. presently, influenza virus a h3n2 and respiratory syncytial virus are the most commonly identified viral pathogens in older adults with viral pneumonia. as diagnostic tests such as reverse-transcription polymerase chain reaction become more widely used, the relative importance of additional viruses (such as parainfluenza, rhinoviruses, coronaviruses, and human metapneumovirus) will ... | 2006 | 16421796 |
| association of human metapneumovirus with acute otitis media. | human metapneumovirus (hmpv) is a recently described paramyxovirus that has been associated with acute upper and lower respiratory infection (lri) in infants and children worldwide. we previously observed that one-third of the children with hmpv-associated lri had been diagnosed with a concomitant acute otitis media (aom). in the current study, we sought to investigate an association between hmpv and children presenting with aom as a primary diagnosis. | 2006 | 16427144 |
| the cytoplasmic domain of the f protein of human respiratory syncytial virus is not required for cell fusion. | the cytoplasmic domains of the fusion proteins encoded by several viruses play a role in cell fusion and contain sites for palmitoylation associated with viral protein trafficking and virus assembly. the fusion (f) protein of human respiratory syncytial virus (hrsv) has a predicted cytoplasmic domain of 26 residues containing a single palmitoylated cysteine residue that is conserved in bovine rsv f protein, but not in the f proteins of other pneumoviruses such as pneumonia virus of mice, human m ... | 2006 | 16432027 |
| effect of ribavirin and glucocorticoid treatment in a mouse model of human metapneumovirus infection. | human metapneumovirus (hmpv)-infected balb/c mice were treated with ribavirin (40 mg/kg of body weight twice a day intraperitoneally), corticosterone (0.2 mg/ml in water), or both modalities. ribavirin significantly decreased both hmpv replication in lungs (by 5 log10) and global pulmonary inflammation on day 5 postinfection, whereas glucocorticoids reduced only alveolar and interstitial inflammation, compared to controls. | 2006 | 16436743 |
| cytotoxic t-lymphocyte epitope vaccination protects against human metapneumovirus infection and disease in mice. | human metapneumovirus (hmpv) has emerged as an important human respiratory pathogen causing upper and lower respiratory tract infections in young children and older adults. in addition, hmpv infection is associated with asthma exacerbation in young children. recent epidemiological evidence indicates that hmpv may cocirculate with human respiratory syncytial virus (hrsv) and mediate clinical disease similar to that seen with hrsv. therefore, a vaccine for hmpv is highly desirable. in the present ... | 2006 | 16439559 |
| seasonal distribution and phylogenetic analysis of human metapneumovirus among children in osaka city, japan. | human metapneumovirus (hmpv) is one of the etiological agents of respiratory tract infection (rti). because clinical symptoms of hmpv resemble those caused by respiratory syncytial virus (rsv), clinical diagnosis of hmpv infection is difficult. moreover, hmpv isolation using cultured cells is generally difficult and not efficient compared with reverse transcription-pcr (rt-pcr). | 2006 | 16460998 |
| office pediatrics: current perspectives on the outpatient evaluation and management of lower respiratory infections in children. | the outpatient evaluation and management of a child with lower respiratory infection (lri) remain a challenge to clinicians worldwide. this update will discuss current problems and new developments in the outpatient evaluation and treatment of pediatric lris. | 2006 | 16470166 |
| novel human metapneumovirus sublineage. | in a pediatric surveillance network, 287 (5.1%) of 5,580 specimens from patients with acute respiratory infections tested positive for human metapneumovirus (hmpv). phylogenetic analysis of n- and f-gene sequences of identified hmpv showed that 30% belonged to a novel phylogenetic cluster. | 2006 | 16494734 |
| human metapneumovirus, peru. | we retrospectively studied 420 pharyngeal swab specimens collected from peruvian and argentinean patients with influenzalike illness in 2002 and 2003 for evidence of human metapneumovirus (hmpv). twelve specimens (2.3%) were positive by multiple assays. six specimens yielded hmpv isolates. four of the 6 isolates were of the uncommon b1 genotype. | 2006 | 16494771 |
| respiratory viral threats. | to assess new information from peer-reviewed publications in 2005 regarding emerging respiratory viral threats. | 2006 | 16514342 |
| brief communication: fatal human metapneumovirus infection in stem-cell transplant recipients. | human metapneumovirus (hmpv), a recently discovered respiratory virus, is associated with clinical disease in young and elderly persons. | 2006 | 16520475 |
| human metapneumovirus: important but not currently diagnosable. | 2006 | 16520481 | |
| human metapneumovirus in paediatric patients. | acute respiratory tract infections (artis) are a leading cause of morbidity and mortality in children worldwide, but the aetiology of many artis is still unknown. in 2001, researchers in the netherlands reported the discovery of a previously unidentified pathogen called human metapneumovirus (hmpv). since its initial description, hmpv has been associated with arti in europe (italy, france, spain, the uk, germany, denmark, finland and norway), america (the usa, canada, argentina and brazil), asia ... | 2006 | 16524405 |
| human metapneumovirus in adults: a short case series. | this study was carried out to further the available information on adult cases of human metapneumovirus (hmpv), a recently described cause of respiratory infection. among a cohort of 741 symptomatic patients tested since 2003, the virus was diagnosed in six adults using reverse transcriptase polymerase chain reaction. of the six, two were from the community, two were hospital inpatients with chronic obstructive pulmonary disease and two were immunocompromised patients, both of whom required vent ... | 2006 | 16534565 |
| beyond viruses: clinical profiles and etiologies associated with encephalitis. | encephalitis is a complex syndrome, and its etiology is often not identified. the california encephalitis project was initiated in 1998 to identify the causes and further describe the clinical and epidemiologic characteristics of encephalitis. | 2006 | 17109290 |
| human metapneumovirus infection among children in taiwan: a comparison of clinical manifestations with other virus-associated respiratory tract infections. | this study compared the clinical, laboratory and radiological features of infections caused by human metapneumovirus (hmpv) with other respiratory viruses. nasopharyngeal aspirates and throat swabs were obtained from children during a 9-week period. hmpv was the virus isolated most frequently, followed by adenovirus, influenza virus a, respiratory syncytial virus and influenza virus b. hmpv-infected children were younger, and were more likely to be female, to present with feeding difficulties, a ... | 2006 | 17121629 |
| viral infections in patients with hematological malignancies. | viral infections are important causes of morbidity and mortality for patients with a hematological malignancy. however, the true incidence and consequences of viral infections for these patients who undergo conventional nontransplant therapy are poorly defined. the difference in incidence and outcome of viral infections among patient groups is wide, but dependent upon the intensity and duration of t-cell-mediated immune suppression. infections caused by cytomegalovirus (cmv), herpes simplex viru ... | 2006 | 17124085 |
| [human metapneumovirus (hmpv) associated to severe bronchial asthmatic crisis]. | human metapneumovirus (hmpv) is a recently reported agent of acute infection in the respiratory tract. it has been found in children as well as in young adults and elders. the clinical manifestations produced by hmpv are indistinguishable from those by common respiratory virus, and can evolve from asymptomatic infection into severe pneumonia. on the other hand, some authors have described cases of bronchial asthma exacerbation associated with hmpv infection. in this work we report a case of a ch ... | 2006 | 17152212 |
| serosurvey of human metapneumovirus infection in croatia. | to assess the seroprevalence of human metapneumovirus (hmpv) in croatia. | 2006 | 17167860 |
| live vaccines for human metapneumovirus designed by reverse genetics. | human metapneumovirus (hmpv) was first described in 2001 and has quickly become recognized as an important cause of respiratory tract disease worldwide, especially in the pediatric population. a vaccine against hmpv is required to prevent severe disease associated with infection in infancy. the primary strategy is to develop a live-attenuated virus for intranasal immunization, which is particularly well suited against a respiratory virus. reverse genetics provides a means of developing highly ch ... | 2006 | 17181442 |
| human metapneumovirus in turkey poults. | this study was conducted to reexamine the hypothesis that human metapneumovirus (hmpv) will not infect turkeys. six groups of 2-week-old turkeys (20 per group) were inoculated oculonasally with 1 of the following: noninfected cell suspension; hmpv genotype a1, a2, b1, or b2; or avian metapneumovirus (ampv) subtype c. poults inoculated with hmpv showed nasal discharge days 4-9 postexposure. specific viral rna and antigen were detected by reverse-transcription pcr and immunohistochemical evaluatio ... | 2006 | 17235379 |
| [first detection of human metapneumovirus in a child with respiratory syndrome in hungary]. | human metapneumovirus was identified in 2001 as a respiratory-tract pathogen that has been classified as a new genera in family paramyxoviridae. | 2006 | 17256630 |
| [human metapneumovirus]. | human metapneumovirus (hmpv), first isolated in the netherlands in 2001, is a member of the genus metapneumovirus of the sub-family pneumovirinae of the family paramyxoviridae. the genomic organization of hmpv is 3'-n-p-m-f-m2-sh-g-l-5'. hmpv resembles the sole member of this genus, avian pneumovirus. hmpv is the most closely related human pathogen to respiratory syncytial virus. phylogenetic analysis of the nucleotide sequences indicated that there were two genetic groups. furthermore, each gro ... | 2006 | 17446666 |
| perspective on the host response to human metapneumovirus infection: what can we learn from respiratory syncytial virus infections? | human metapneumovirus (hmpv) is a recently discovered pathogen first identified in respiratory specimens from young children suffering from clinical respiratory syndromes ranging from mild to severe lower respiratory tract illness. hmpv has worldwide prevalence, and is a leading cause of respiratory tract infection in the first years of life, with a spectrum of disease similar to respiratory syncytial virus (rsv). the disease burden associated with hmpv infection has not been fully elucidated; h ... | 2006 | 16182587 |
| human metapneumovirus in infants and young children in thailand with lower respiratory tract infections; molecular characteristics and clinical presentations. | human metapneumovirus (hmpv) is a recently identified paramyxovirus. the clinical features and molecular characteristics of hmpv in asian populations have so far remained obscure. | 2006 | 16183133 |
| differential response of dendritic cells to human metapneumovirus and respiratory syncytial virus. | dendritic cells (dcs) play a pivotal role in shaping antiviral immune responses in the respiratory tract. human metapneumovirus (hmpv) is a recently identified pathogen and like its better known relative, respiratory syncytial virus (rsv), has been increasingly recognized as a major cause of respiratory morbidity in infants and in elderly persons. in the present study, we examined susceptibility as well as cellular responses of human dcs to hmpv compared with rsv. monocyte-derived dcs (modcs) we ... | 2006 | 16284360 |
| simultaneous detection and typing of human metapneumovirus strains in nasopharyngeal secretions and cell cultures by monoclonal antibodies. | 2006 | 16310406 | |
| rates of hospitalisation for influenza, respiratory syncytial virus and human metapneumovirus among infants and young children. | to inform the development of a national influenza immunisation programme and the potential role of antiviral drugs in young children, we studied 613 children aged 71 months or younger who attended leicester childrens' hospital during winter 2001-2002. during periods of respiratory syncytial virus (rsv), influenza, and human metapneumovirus activity, an estimated 12.2% (95% ci: 11.4-13.1), 7.1% (6.3-7.9), and 2.5% (2.1-2.9), respectively, of all medical cases assessed in the hospital were associa ... | 2006 | 16310899 |
| detection of respiratory syncytial virus and human metapneumovirus by reverse transcription polymerase chain reaction in adults with and without respiratory illness. | reverse transcription polymerase chain reaction (rt-pcr) is a powerful tool that allows the detection of minute quantities of viral rna. because of the sensitivity of these assays it is possible that the finding of viral rna indicates not only active infection but also transient colonization or residual nucleic acid from a distant infection. respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) are two viruses for which rt-pcr is now frequently used for diagnosis in adult disease. | 2006 | 15919236 |
| detection and pathogenicity of human metapneumovirus respiratory infection in pediatric italian patients during a winter--spring season. | some diagnostic, epidemiological and clinical features of the recently discovered human metapneumovirus remain to be investigated. | 2006 | 16023411 |
| the viral bronchiolites diagnosis in children by pcr multiplex. | the aim of the study was to determine the etiology of the viral bronchiolites in children by using direct immunofluorescence test and 3 rt-pcr multiplex (s.bellau-pujol) the study was performed on 122 nasal inspirations collected from 3 weeks-6 month old children hospitalizated in the pediatrics service of ch rouen. the results were that the majority (53%) of bronchiolites in children had like etiology rsv and a lot of these infections had double viral etiology (26% rsv+ rhinovirus; 2,7% rsv+hmp ... | 2006 | 18389721 |
| informing the front line about common respiratory viral epidemics. | the nature of clinical medicine is to focus on individuals rather than the populations from which they originate. this orientation can be problematic in the context of acute healthcare delivery during routine winter outbreaks of viral respiratory disease where an individuals likelihood of viral infection depends on knowledge of local disease incidence. the level of interest in and perceived utility of community and regional infection data for front line clinicians providing acute care is unclear ... | 2007 | 18693841 |
| first detection of human metapneumovirus in children with respiratory infections in romania. | the human metapneumovirus (hmpv) was first isolated in 2001 in the netherlands (van der hoogen and collaborators) from a nasopharyngeal aspirate sampled from an infant. based on the morphological, biochemical and genetic characteristics, the hmpv was initially classified in the genus metapneumovirus with the avian metapneumovirus (apv), the agent causing the respiratory infections of the upper tract in turkeys and other birds. subsequently, together with the respiratory syncytial virus (rsv), it ... | 2007 | 18928061 |