Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| effects of preservatives and killing methods on morphological features of a forensic fly, chrysomya megacephala (fabricius, 1794) larva. | preservation of larvae retrieved from cadavers is important in ensuring the quality and integrity of entomological specimens used for the estimation of post-mortem interval (pmi). the process of killing and preserving larvae could distort the larvae leading to inaccurate estimation of pmi. in this study, the effects of killing chrysomya megacephala larvae with hot water at different temperatures and subsequent maintenance in various preservatives were determined. larvae not killed by hot water b ... | 2014 | 25776605 |
| an atpase inhibitory peptide with antibacterial and ion current effects. | an 84-residue bactericidal peptide, psk, was purified from a chrysomya megacephala fly larvae preparation. its amino acid sequence is similar to that of a previously reported larval peptide of the drosophila genus (sk84) noticed for its anticancer and antimicrobial properties. the psk sequence is also homologous to mitochondrial atpase inhibitors from insects to humans (35-65% sequence identity), indicating an intracellular protein target and possible mechanism for psk. it contains a cluster of ... | 2014 | 24613831 |
| larval distribution and behavior of chrysomya rufifacies (macquart) (diptera: calliphoridae) relative to other species on florida black bear (carnivora: ursidae) decomposing carcasses. | larval interactions of dipteran species, blow flies in particular, were observed and documented daily over time and location on five black bear carcasses in gainesville, fl, usa, from june 2002 - september 2004. cochliomyia macellaria (fabricius) or chrysomya megacephala (fabricius) larvae were collected first, after which chrysomya rufifacies (macquart) oviposited on the carcasses in multiple locations (i.e., neck, anus, and exposed flesh) not inhabited already by the other blow fly larvae. wit ... | 2014 | 27193400 |
| the developmental transcriptome of the synanthropic fly chrysomya megacephala and insights into olfactory proteins. | chrysomya megacephala (fabricius) is a prevalent and synanthropic blowfly which has two sides, for being a pathogenic vector, an efficient pollinator, a promising resource of proteins, lipids, chitosan, biofuel et al., and an important forensic indicator. moreover olfactory proteins are crucial component to function in related processes. however, the genomic platform of c. megacephala remains relatively unavailable. developmental transcriptomes of eggs, larvae from 1st instar to before pupa stag ... | 2015 | 25612629 |
| an evaluation of sampling methods used to produce insect growth models for postmortem interval estimation. | many authors produced carrion insect development data for predicting the age of an insect from a corpse. under some circumstances, this age value is a minimum postmortem interval. there are no standard protocols for such experiments, and the literature includes a variety of sampling methods. to our knowledge, there has been no investigation of how the choice of sampling method can be expected to influence the performance of the resulting predictive model. we calculated 95 % inverse prediction co ... | 2015 | 24929639 |
| human myiasis in rural south africa is under-reported. | myiasis is the infestation of live tissue of humans and other vertebrates by larvae of flies. worldwide, myiasis of humans is seldom reported, although the trend is gradually changing in some countries. reports of human myiasis in africa are few. several cases of myiasis were recently seen at the mthatha hospital complex, mthatha, eastern cape province, south africa (sa). | 2015 | 26242532 |
| effect of juvenoids pyriproxyfen and diofenolan on embryogenesis and postembryonic development of blow fly chrysomya megacephala (diptera: calliphoridae) following egg treatment. | the blow fly chrysomya megacephala is a serious medico-veterinary pest causing myiasis in humans and animals apart from transmitting various disease-causing pathogens. being an economically important pest, it warrants successful control in the early stages without affecting the host, environment, and non-target organisms. juvenoids being safe, species and stage selective, biodegradable, and harmless to beneficial non-target organisms. treatment of freshly laid eggs of c. megacephala with juvenoi ... | 2015 | 26003811 |
| survey of the genetic diversity of forensically important chrysomya (diptera: calliphoridae) from egypt. | minimum postmortem interval estimations of a corpse using blow fly larvae in medicolegal investigations require correct identification and the application of appropriate developmental data of the identified fly species. species identification of forensically relevant blow flies could be very difficult and time consuming when specimens are damaged or in the event of morphologically indistinguishable immature stages, which are most common at crime scenes. in response to this, an alternative, accur ... | 2015 | 26334805 |
| multiple species of scuttle flies (diptera: phoridae) as contaminants in forensic entomology laboratory insect colony. | in forensic entomology, larval rearing usually includes the presence of biological contaminants including scuttle flies (diptera: phoridae). scuttle flies are recognized as forensically important insects and have been reported causing nuisance and contamination in laboratory environments. this paper reports for the first time the finding of multiple scuttle fly species affecting colonies of third instar larvae of the oriental latrine blowfly, chrysomya megacephala (fabricius) (diptera: calliphor ... | 2015 | 26695221 |
| forensic entomology of high-rise buildings in malaysia: three case reports. | the distributions of flies are not only confined to ground level but can also be at higher altitudes. here, we report three forensic cases involving dipterans in high-rise buildings in kuala lumpur, malaysia. case 1 involved a corpse of adult female found at the top floor of a fifteen-story apartment. case 2 dealt with a body of a 75-year-old female discovered in a bedroom on the eleventh floor of an eighteen-story building, while case 3 was a 52-year-old male found in his fifth floor shop house ... | 2015 | 26691258 |
| edge effects on the blowfly fauna (diptera, calliphoridae) of the tijuca national park, rio de janeiro, brazil. | in this contribution we examine the diversity, abundance and species richness of calliphoridae in the tijuca national park, rio de janeiro, rj. specimens were collected monthly between september 2009 and august 2010, using sardines as bait, in three points: a, on the forest edge (22°56'846"s 43°17'496"w), b, 700 m from it (22°57'073"s 43°17'832"w) and c, 1,200 m into the forest (22°57'321"s 43°18'031"w), evaluating the indicator species at each point and the anthropogenic influences and abiotic ... | 2015 | 26675918 |
| edge effects on the blowfly fauna (diptera, calliphoridae) of the tijuca national park, rio de janeiro, brazil. | abstractin this contribution we examine the diversity, abundance and species richness of calliphoridae in the tijuca national park, rio de janeiro, rj. specimens were collected monthly between september 2009 and august 2010, using sardines as bait, in three points: a, on the forest edge (22°56'846"s 43°17'496"w), b, 700m from it (22°57'073"s 43°17'832"w) and c, 1,200m into the forest (22°57'321"s 43°18'031"w), evaluating the indicator species at each point and the anthropogenic influences and ab ... | 2015 | 26560666 |
| reference gene stability of a synanthropic fly, chrysomya megacephala. | stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qpcr). a synanthropic fly, chrysomya megacephala, is a well known medical vector and forensic indicator. unfortunately, previous studies did not look at the stability of reference genes used in c. megacephala. | 2015 | 26515169 |
| body size, rather than male eye allometry, explains chrysomya megacephala (diptera: calliphoridae) activity in low light. | male chrysomya megacephala (f.) blow fly compound eyes contain an unusual area of enlarged dorsal facets believed to allow for increased light capture. this region is absent in females and has been hypothesized to aid in mate tracking in low light conditions or at greater distances. many traits used in the attraction and capture of mates are allometric, growing at different rates relative to body size. previous reports concerning c. megacephala eye properties did not include measurements of body ... | 2015 | 26411786 |
| the importance of habitat in the ecology of decomposition on rabbit carcasses in malaysia: implications in forensic entomology. | the stages of decomposition and the faunal succession on rabbit carcasses in three different habitats, namely jungle, rural, and highland areas, were studied. three new zealand white rabbit (oryctolagus cuniculus) carcasses weighing ∼2 kg were sampled daily until the decomposition process was completed. representative specimens of adult flies, larvae, pupa, and mites were collected from the carcasses and processed in the laboratory. there were differences in decomposition rate and faunal success ... | 2015 | 26336275 |
| egg developmental time and survival of chrysomya megacephala and chrysomya putoria (diptera: calliphoridae) under different temperatures. | chrysomya megacephala (f.) and chrysomya putoria (wiedemann) (diptera: calliphoridae) are considered of forensic, medical, and veterinary importance in brazil because of their necrophagous and synanthropic behaviour. the development of flies can be influenced by temperature, and species from the same genus usually have different responses to external variables. the egg development of blow fly can be a useful complementary technique to estimate the minimum postmortem interval. thus, this study ai ... | 2015 | 26335461 |
| preliminary screening of the larvicidal effect of brevibacillus laterosporus strains against the blowfly chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae). | this study evaluated whether different strains of brevibacillus laterosporus could be used to control larvae of the blowfly chrysomya megacephala, a pest that affects both human and animal health. | 2015 | 26312933 |
| use of maggot therapy for treating a diabetic foot ulcer colonized by multidrug resistant bacteria in brazil. | this study reports the efficacy of maggot therapy in the treatment of diabetic foot ulcer infected with multidrug resistant microorganisms. a 74 year old female patient with diabetes for over 30 years, was treated with maggot therapy using larvae of chrysomya megacephala. the microbiological samples were collected to evaluate aetiology of the infection. the therapy done for 43 days resulted in a reduction of necrosis and the ulcer's retraction of 0.7 cm [2] in area. analysis of the bacteriologic ... | 2015 | 25963495 |
| detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of chrysomya (diptera, calliphoridae) in brazil. | antibiotic-resistant bacteria in hospitals and communities increasingly threaten public health in brazil and the rest of the world. there is an urgent need for additional antimicrobial drugs. calliphorid blowfly larvae are a rich source of antimicrobial factors but the potential of neotropical species has been neglected. this preliminary study evaluates the antimicrobial activity of the native excretions/secretions of larvae of three species of brazilian calliphorids, chrysomya megacephala, chry ... | 2015 | 25817237 |
| unusual presence of ornidia robusta (diptera: syrphidae) causing pig myiasis in argentina. | myiasis is caused by dipterous larvae from the calliphoridae, sarcophagidae, muscidae, cuterebridae, and syrphidae families. in this work, cochliomyia hominivorax, chrysomya megacephala, and ornidia robusta were identified causing vulva, ear, and leg myiasis in pigs in tucuman province, northwestern argentina. the report of the presence of c. hominivorax and c. megacephala is very important due to their role as myiasis-causing and disease vectors. the occurrence of ornidia robusta is remarkable, ... | 2015 | 26450595 |
| in vitro repellent effect of tea tree (melaleuca alternifolia) and andiroba (carapa guianensis) oils on haemotobia irritans and chrysomya megacephala flies. | this study aimed to evaluate the repellent effect of tea tree (melaleuca alternifolia) and andiroba (carapa guianensis) essential oils on two species of flies (haemotobia irritans and chrysomya megacephala). for the in vitro studies, free-living adult flies were captured and reared in the laboratory. to verify the repellency effect, an apparatus was constructed where h. irritans and c. megacephala were exposed to andiroba and tea tree oils (5.0%), as well as to a known repellent (citronella, 5.0 ... | 2015 | 25801266 |
| reading mammal diversity from flies: the persistence period of amplifiable mammal mtdna in blowfly guts (chrysomya megacephala) and a new dna mini-barcode target. | most tropical mammal species are threatened or data-deficient. data collection is impeded by the traditional monitoring approaches which can be laborious, expensive and struggle to detect cryptic diversity. monitoring approaches using mammal dna derived from invertebrates are emerging as cost- and time-effective alternatives. as a step towards development of blowfly-derived dna as an effective method for mammal monitoring in the biodiversity hotspot of peninsular malaysia, our objectives were (i ... | 2015 | 25898278 |
| antibacterial activity of excretions-secretions from chrysomya megacephala against escherichia coli. | the blowfly, chrysomya megacephala, is distributed worldwide. previous studies found maggot excretions-secretions from other blowfly species inhibited pro-inflammatory response and antimicrobial activity. | 2016 | 26817232 |
| decomposition of concealed and exposed porcine remains in the north carolina piedmont. | we examined the decomposition and subsequent insect colonization of small pig carrion (sus scrofa (l.)) placed in concealed and open environments during spring, summer, and fall in raleigh, north carolina, as a model for juvenile human remains. remains were concealed in simulated attics in three manners, ranging from minimal to well-concealed. concealment had a significant effect on the insect community colonizing the remains across all three seasons; the beetles necrobia rufipes (degeer) (cleri ... | 2016 | 26590193 |
| evaluating the effects of different vegetation types on necrophagous fly communities (diptera: calliphoridae; sarcophagidae): implications for conservation. | the present study was conducted in five different phytogeographic zones of the brazilian state of maranhão, three of which (the amazon forest, cerrado, and palm groves) are more heterogeneous, whereas the other two (marshlands and mangroves) are more homogeneous. in each zone, nine sites were visited for the collection of necrophagous flies using bait traps in 2010, 2011, and 2012. the calliphorid and sarcophagid communities observed at each site were compared in terms of species richness, compo ... | 2016 | 27798664 |
| developmental times of chrysomya megacephala (fabricius) (diptera: calliphoridae) at constant temperatures and applications in forensic entomology. | the characteristic life stages of infesting blowflies (calliphoridae) such as chrysomya megacephala (fabricius) are powerful evidence for estimating the death time of a corpse, but an established reference of developmental times for local blowfly species is required. we determined the developmental rates of c. megacephala from southwest china at seven constant temperatures (16-34°c). isomegalen and isomorphen diagrams were constructed based on the larval length and time for each developmental ev ... | 2016 | 27581209 |
| dataset of traumatic myiasis observed for three dominant screw worm species in north west pakistan with first report of wohlfahrtia magnifica (schiner). | regional surveys were carried out in different parts of north west pakistan among domestic animals (n=57,921) including pets and livestock identifying cases of traumatic myiasis (n=1037). a total of four surveys focused general livestock population during eid ul adha (eid surveys; incidence=1.21%) while another four surveys (miscellaneous surveys; incidence=7.34%) targeted animal population brought to veterinary hospitals and dispensaries. timeframe spanned four years from 2012 to 2015. maggots ... | 2016 | 27570812 |
| volume of larvae is the most important single predictor of mass temperatures in the forensically important calliphorid, chrysomya megacephala (diptera: calliphoridae). | calliphorid species form larval aggregations that are capable of generating heat above ambient temperature. we wanted to determine the relationship between volume, number of larvae, and different combinations of instars on larval mass heat generation. we compared different numbers of chrysomya megacephala (f.) larvae (40, 100, 250, 600, and 2,000), and different combinations of instars (∼50/50 first and second instars, 100% second instars, ∼50/50 second and third instars, and 100% third instars) ... | 2016 | 27550079 |
| the classic episode of biological invasion: cochliomyia macellaria (fabricius, 1775) versus chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) - evaluation of the biotic potential. | the classic episode of biological invasion: cochliomyia macellaria (fabricius, 1775) versus chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) - evaluation of the biotic potential. the biotic of the native fly cochliomyia macellaria and the exotic chrysomya megacephala was compared, through the reproductive capacity and longevity. the experiment took place in a climatized chamber, with four repetitions of 15 grouped couples of each species. the posture, egg mass weight, and death o ... | 2016 | 27508990 |
| the forensically important blow fly, chrysomya megacephala (diptera: calliphoridae), is more likely to walk than fly to carrion at low light levels. | one factor that influences estimates of time since death using entomological evidence is whether or not blow flies nocturnally oviposit. field studies focusing on egg laying have found it occurs on an inconsistent basis. a key but poorly understood factor in nocturnal oviposition is a blow fly's ability to locate carrion under low light levels. it has been speculated that blow flies are more likely to walk than fly to carrion during the night, but this has not been empirically tested. we directl ... | 2016 | 27343754 |
| seasonal, locality, and habitat variation in assemblages of carrion-associated diptera in gauteng province, south africa. | seasonal, spatial, and habitat responses of carrion-associated diptera assemblages can provide valuable information about the presence or absence of species and their relative abundance, and thereby enhance understanding of their responses to environmental variables and how this may have an impact on forensic investigations. three different nature reserves (localities) within the municipality of tshwane, south africa, were selected to determine whether species assemblages of carrion-feeding flie ... | 2016 | 27330098 |
| chrysomya megacephala (fabricius) (diptera: calliphoridae) oviposition behavior in previous oviposition situation. | blowflies use their sense of smell to locate oviposition substrates and their vision to direct and assist in landing decision. blowflies' ovipositions are usually aggregated. chrysomya megacephala (fabricius) females, for example, prefer to lay eggs on substrates containing fresh eggs of the same species. however, it is possible that females would be capable of evaluating the substrate, reducing the size of egg cluster in sites with a high amount of eggs or larvae, or finding another site for la ... | 2016 | 27295050 |
| the effect of flunitrazepam (rohypnol(®) ) on the development of chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) and its implications for forensic entomology. | this study investigated the potential effects of flunitrazepam (known as "date rape drug") on the developmental cycle of chrysomya megacephala, an important forensic species, and their possible implications for the calculation of the pmi. a 1050 c. megacephala eggs were divided into five groups with seven replications each. the eggs were placed on artificial diet prepared with four drug concentrations of flunitrazepam (4, 8, 16, and 32 ng/g), besides the control group (prepared with water). were ... | 2016 | 27143233 |
| assemblage of necrophagous diptera in atlantic insular environments and response to different levels of human presence. | islands act as natural laboratories for ecological studies to explain bioinvasion processes and, in this scenario, necrophagous diptera have never been used as model organisms. this study aimed to (i) describe assemblages of necrophagous diptera (calliphoridae and sarcophagidae) in two insular environments of different origins and distances from mainland, (ii) investigate the effect of anthropogenic impact on the assemblage of carrion flies, (iii) to quantify the establishment of invasive specie ... | 2016 | 27040531 |
| pandora bullata (entomophthoromycota: entomophthorales) affecting calliphorid flies in central brazil. | fungi are where one finds them, and if one seeks fungal pathogens affecting flies, then a garbage dump may be an ideal place to find both persistent, abundant fly populations and their fungal pathogens. an obvious fungal epizootic affecting the oriental latrine fly, chrysomya megacephala (diptera: calliphoridae), was observed over several days in mid-february 2015 at the local garbage dump adjacent to the city of cavalcante, northern goiás. this site harbored large populations of both c. megacep ... | 2016 | 26968351 |
| large-scale mitogenomics enables insights into schizophora (diptera) radiation and population diversity. | true flies are insects of the order diptera and encompass one of the most diverse groups of animals on earth. within dipterans, schizophora represents a recent radiation of insects that was used as a model to develop a pipeline for generating complete mitogenomes using various sequencing platforms and strategies. 91 mitogenomes from 32 different species were sequenced and assembled with high fidelity, using amplicon, whole genome shotgun or single molecule sequencing approaches. based on the nov ... | 2016 | 26912394 |
| patterns of oviposition and development of chrysomya megacephala (fabricius) (diptera: calliphoridae) and chrysomya rufifacies (macquart) (diptera: calliphoridae) on burned rabbit carcasses. | considering that crimes against animals such as illegal killing and cruelty have been alarmingly increasing and since burning is one of the common ways for disposing cadavers, ability to estimate minimum postmortem interval (pmi) using entomological data merits consideration. chrysomya megacephala and chrysomya rufifacies are common necrophagous species recovered from cadavers in many countries including malaysia. specific studies focusing on the oviposition and developmental patterns of both sp ... | 2016 | 26779962 |
| assessment of the abundance and diversity of calliphoridae and sarcophagidae (diptera) in sites with different degrees of human impact in the iberá wetlands (argentina). | the aim of the present study was to explore the diversity and describe the spatial distribution of calliphoridae and sarcophagidae along an urbanization gradient in the iberá wetlands. the flies were collected at 18 sampling points, three per site. in total, 3,318 flies were collected (3,077 blow flies and 241 flesh flies), distributed in 13 genera and 33 species. calliphoridae was the most abundant, comprised 92.74% of all adult flies. chrysomya albiceps (wiedemann), cochliomyia macellaria (f.) ... | 2016 | 27106933 |
| intraguild predation influences oviposition behavior of blow flies (diptera: calliphoridae). | the objective of the present study was to determine whether blow flies (diptera: calliphoridae) are able to identify larvae of an intraguild predator species in the substrate and avoid laying eggs there. blow flies oviposited in traps with different treatments: substrate only and substrate with larvae of chrysomya albiceps (wiedemann, 1819), chrysomya megacephala (fabricius, 1794), or chrysomya putoria (wiedemann, 1830). ch. megacephala, ch. putoria, and lucilia eximia (wiedemann, 1819) avoided ... | 2016 | 26888288 |
| effect of bait decomposition on the attractiveness to species of diptera of veterinary and forensic importance in a rainforest fragment in brazil. | insects associated with carrion can have parasitological importance as vectors of several pathogens and causal agents of myiasis to men and to domestic and wild animals. we tested the attractiveness of animal baits (chicken liver) at different stages of decomposition to necrophagous species of diptera (calliphoridae, fanniidae, muscidae, phoridae and sarcophagidae) in a rainforest fragment in brazil. five types of bait were used: fresh and decomposed at room temperature (26 °c) for 24, 48, 72 an ... | 2016 | 26547564 |
| first report of oriental latrine flies causing vaginal myiasis in human. | the present paper reports a case of human vaginal myiasis in a 22 year old woman. this is the first report of this fly species to cause vaginal myiasis in human till now. the infested maggots were removed from the vagina and cultured in laboratory. entomological studies on the emerged flies showed that the infested larvae were oriental latrine fly chrysomya megacephala fabricius. clinical presentation and treatment strategies are discussed also. | 2016 | 27876924 |
| development of the oriental latrine fly, chrysomya megacephala (diptera: calliphoridae), at five constant temperatures. | chrysomya megacephala (fabricius) is a forensically important fly that is found throughout the tropics and subtropics. we calculated the accumulated development time and transition points for each life stage from eclosion to adult emergence at five constant temperatures: 15, 20, 25, 30, and 35 °c. for each transition, the 10th, 50th, and 90th percentiles were calculated with a logistic linear model. the mean transition times and % survivorship were determined directly from the raw laboratory dat ... | 2017 | 27816915 |
| species composition of carrion blow flies in northern thailand: altitude appraisal. | distribution and occurrence of blow flies of forensic importance was performed during 2007 and 2008 in chiang mai and lampang provinces, northern thailand. surveys were conducted in forested areas for 30 minutes using a sweep net to collected flies attracted to a bait. a total of 2,115 blow flies belonging to six genera and 14 species were collected; chrysomya megacephala (fabricius) (44.7%), c. pinguis (walker) (15.1%), c. chani kurahashi (9.3%), c. thanomthini kurahashi & tumrasvin (0.3%); ach ... | 2017 | 24626423 |
| laboratory evaluation of brevibacillus laterosporus strains as biocidal agents against chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) larvae. | the biocidal activity of three strains of brevibacillus laterosporus upon the post-embryonic developmental stages of chrysomya megacephala was evaluated. bioassays were performed to verify lethal and sub-lethal effects including ultra-structural changes in the midgut. among the strains assayed, shi3 presented the highest larval mortality rates, achieving 70% at a concentration of 1×10(8) spores/g of diet. transmission electron microscopy revealed intestinal alterations caused by all strains test ... | 2017 | 28442400 |
| nocturnal oviposition behavior of blowflies (diptera: calliphoridae) in the southern hemisphere (south africa and australia) and its forensic implications. | published research has offered contradictory evidence of the occurrence of nocturnal oviposition by carrion-breeding blowflies, a behavior that can affect the interpretation of forensic estimates of a minimum post mortem interval (minpmi) by up to 12 hours, depending on latitude and season. the majority of published studies are from the northern hemisphere. field experiments were conducted in south africa and australia that extend observations to species of the southern hemisphere. various verte ... | 2017 | 28409386 |
| forensic entomotoxicology revisited-towards professional standardisation of study designs. | forensic entomotoxicology is the use of insects as evidence of whether a toxicant is present in an environment such as a corpse, river or landscape. the earliest overtly forensic study was published in 1977, and since then, at least 63 papers have been published, most of them focused on the detection of toxicants in insects or on effects of toxicants on diverse insect indicator taxa. a comprehensive review of the published literature revealed various inconsistencies between studies that could be ... | 2017 | 28567525 |
| ultrastructure of male genitalia of blow flies (diptera: calliphoridae) of forensic importance. | male genitalia of blow flies (diptera: calliphoridae) are distinctive in their morphological features and are often used for species identification. the aim of this work was to investigate the male genitalia of blow flies of medical and forensic importance from thailand at the ultrastructural level, using scanning electron microscopy (sem). flies in two subfamilies were examined: chrysomyinae [chrysomya bezziana villeneuve, chrysomya chani kurahashi, chrysomya nigripes aubertin, chrysomya pingui ... | 2017 | 29248415 |
| dna-based identification of forensically important blow flies (diptera: calliphoridae) from india. | correct species identification is the first and the most important criteria in entomological evidence-based postmortem interval (pmi) estimation. although morphological keys are available for species identification of adult blow flies, keys for immature stages are either lacking or are incomplete. in this study, cytochrome oxidase subunit 1 (coi) reference data were developed from nine species (belonging to three subfamilies, namely, calliphorinae, luciliinae, and chrysomyinae) of blow flies fro ... | 2017 | 28419283 |
| longevity, fertility and fecundity of adult blow flies (diptera: calliphoridae) held at varying densities: implications for use in bioconversion of waste. | large numbers of flies are needed to produce the quantity of larvae required for insect bioconversion of waste. however, this 'mass-rearing' may negatively affect adult survival and reproductive output. this study assessed the suitability for mass-rearing of four blow fly species, chrysomya chloropyga, chrysomya chloropyga (wiedemann), chrysomya megacephala (f.), chrysomya putoria (wiedemann) and lucilia sericata (meigen). flies were kept at densities of 20, 50, 100, 250, 500, and 1,000 flies pe ... | 2017 | 29040631 |
| wohlfahrtiimonas chitiniclastica: current insights into an emerging human pathogen. | since the first description of wohlfahrtiimonas chitiniclastica in 2008, a number of well described case reports demonstrating its pathogenic role in humans have been published. infections may be closely linked to flies, such as wohlfahrtia magnifica, lucilia sericata, chrysomya megacephala or musca domestica. these insects are potent vectors for the distribution of w. chitiniclastica causing local or systemic infections originating from wounds infested with fly larvae. however, other potential ... | 2017 | 28162132 |
| volume of larvae is the most important single predictor of mass temperatures in the forensically important calliphorid, chrysomya megacephala (diptera: calliphoridae). | calliphorid species form larval aggregations that are capable of generating heat above ambient temperature. we wanted to determine the relationship between volume, number of larvae, and different combinations of instars on larval mass heat generation. we compared different numbers of chrysomya megacephala (f.) larvae (40, 100, 250, 600, and 2,000), and different combinations of instars (∼50/50 first and second instars, 100% second instars, ∼50/50 second and third instars, and 100% third instars) ... | 2017 | 28082629 |
| chemotaxonomic profile and intraspecific variation in the blow fly of forensic interest chrysomya megacephala (diptera: calliphoridae). | necrophagous insects such as blow flies (diptera: calliphoridae) are considered crucial in forensic entomology. identification at species level and determination of larval stage are the basis for estimation of postmortem interval (pmi). insect evidence can also be used in the determination of crime scenes, since body displacement is common. the aim of this study was to determine the chemotaxonomic profile and intraspecific variability of the forensically important blow fly chrysomya megacephala ... | 2017 | 28082627 |
| species-level identification of the blowfly chrysomya megacephala and other diptera in china by dna barcoding. | the blowfly chrysomya megacephala, or oriental latrine fly, is the most common human-associated fly of the oriental and australasian regions. chrysomya megacephala is of particular interest for its use in forensic entomology and because it is a disease vector. the larvae are economically important as feed for livestock and in traditional chinese medicine. identification of adults is straightforward, but larvae and fragments of adults are difficult to identify. we collected c. megacephala, its al ... | 2017 | 28044459 |
| isolation of the male-specific transformer exon as a method for immature specimen sex identification in chrysomya megacephala (diptera: calliphoridae). | being able to efficiently differentiate between male and female individuals in the immature forms of insects allows for investigations into sexually dimorphic patterns of growth rates and gene expression. for species lacking sex-specific morphological characteristics during these periods, alternative methods must be devised. commonly, isolation of sex determination genes reveals sex-specific band patterns and allows for markers that can be used in insect control. for blow flies, a family that in ... | 2017 | 28031348 |
| comparative fly species composition on indoor and outdoor forensic cases in malaysia. | forensic entomology refers to the science of collection and analysis of insect evidence in order to determine the minimum time period since death. this study aimed to investigate the occurrence of forensically important flies on 34 human remains referred to universiti kebangsaan malaysia medical centre over a period of three years. entomological specimens were collected at the death scenes and/or during autopsies. live specimens were reared into adults while preserved specimens were processed fo ... | 2017 | 27997861 |
| quantifying pteridines in the heads of blow flies (diptera: calliphoridae): application for forensic entomology. | in forensic cases involving entomological evidence, establishing the postcolonization interval (post-ci) is a critical component of the investigation. traditional methods of estimating the post-ci rely on estimating the age of immature blow flies (diptera: calliphoridae) collected from remains. however, in cases of delayed discovery (e.g., when remains are located indoors), these insects may have completed their development and be present in the environment as adults. adult fly collections are o ... | 2017 | 28942377 |
| insect succession on pig carcasses using different exposure time - a preliminary study in guangzhou, china. | the exposure time of carcasses in insect succession studies are generally not consistent and more than 40% studies start in the morning. how such an arrangement affects the succession of insects is worth evaluating. in this study, six piglet carcasses (15-17 kg) were exposed at 6 time points during the day: 11:00, 15:00, 19:00, 23:00, 03:00 and 07:00 on july 20th-july 21st 2006 in guangzhou, china to investigate the potential effects of the time of day of carcass exposure on body decomposition a ... | 2017 | 28843096 |
| field evaluation of a semi-automatic funnel trap targeted the medically important non-biting flies. | bait-trapping is a useful approach for monitoring fly population dynamics, and it is an effective tool for physical control of pest species. the aim of this study was to test a newly developed semi-automatic funnel fly trap with some modifications of the former prototype fly trap to study medically important fly population density. the efficacy of the semi-automatic funnel trap was assessed by field sampling during july 2013-june 2014 using 1-day tainted beef offal as bait. the modified semi-aut ... | 2017 | 28743450 |
| variation in the time of colonization of broiler carcasses by carrion flies in nakhonsawan province, thailand. | carrion flies are the primary insects colonizing vertebrate carrion; however, limited information is available on the variation in the time of colonization (toc) as related to time of placement (top) and time of death (tod), particularly in thailand. three seasonal sets of nine broiler carcasses (euthanized and placed in field within 0.5 h after death) were placed in mesh enclosures within a disturbed deciduous dipterocarp forest at nakhonsawan province, upper-central thailand, for 3 d to determ ... | 2017 | 28444209 |
| sterilization of blow fly eggs, chrysomya megacephala and lucilia cuprina, (diptera: calliphoridae) for maggot debridement therapy application. | maggot debridement therapy (mdt) is an application of sterile laboratory-reared blow fly larvae to remove necrotic tissue and disinfect wounds for medical conditions. for effective application, the blow fly larvae used in the wound treatment are required to be in aseptic condition. here, we report the results of a detailed assessment of bacteria and fungi isolated from the eggs of two blow fly species, chrysomya megacephala (f.) and lucilia cuprina (wiedemann) before and after sterilization by d ... | 2017 | 28361272 |
| diversity of sarcosaprophagous calyptratae (diptera) on sandy beaches exposed to increasing levels of urbanization in brazil. | sandy beaches are among the most impacted ecosystems worldwide, and the effects of urbanization on the biodiversity of these habitats are largely unknown, particularly in brazil. we investigated the composition and structure of assemblages of sarcosaprophagous insects (diptera: calliphoridae, sarcophagidae, and muscidae) on six sandy beaches exposed to differential levels of human impact in pernambuco state, brazil. in total, 20,672 adults of 40 species were collected, of which 70% were callipho ... | 2017 | 28369231 |
| a preliminary study of insect succession in al-ahsaa oasis, in the eastern region of the kingdom of saudi arabia. | rabbit carcass decomposition was examined in four seasons in al-ahsaa oasis. decomposition rate was significantly faster in summer compared with other seasons. fourteen insect species from four orders and seven families were recorded: calliphoridae, muscidae, sarcophagidae, formicidae, histeridae, dermestidae, and tenebrionidae. most of the fly maggots collected were calliphoridae with only one species from both muscidae and sarcophagidae. maggots of calliphora vicina (r.-d.) and chrysomya albic ... | 2017 | 27861898 |
| analysis of the effect of cyclophosphamide and methotrexate on chrysomya megacephala (diptera: calliphoridae). | forensic entomotoxicology investigates the effects of chemical substances in the development of scavenger insects and the reflection on estimating the minimum postmortem interval (pmi). to evaluate the impact of the chemotherapeutic drugs cyclophosphamide (cf) and methotrexate (mtx) on the postembryonic development of chrysomya megacephala, controlled experiments were performed by rearing the larvae on spiked minced beef with different concentrations of these drugs. the results indicated that cf ... | 2018 | 29351362 |
| can escherichia coli fly? the role of flies as transmitters of e. coli to food in an urban slum in bangladesh. | to investigate the transmission of faecal bacteria by flies to food under natural settings. | 2018 | 29121443 |