Publications

successful medical management of presumptive pythium insidiosum keratitis.	to describe the previously unreported successful treatment of presumptive pythium keratitis (pk) with medical therapy alone.	2017	28207431
evolution of the sterol biosynthetic pathway of pythium insidiosum and related oomycetes contributes to antifungal drug resistance.	pythiosis is a life-threatening infectious disease caused by the oomycete pythium insidiosum direct exposure to py. insidiosum zoospores can initiate infections of the eye, limb, gastrointestinal tract, or skin/subcutaneous tissue. treatments for pythiosis have mostly relied on surgery. antifungal drugs are generally ineffective against py. insidiosum however, one patient with an invasive py. insidiosum infection recovered completely following treatment with terbinafine and itraconazole. additio ...	2017	28115356
description of three novel lagenidium (oomycota) species causing infection in mammals.	recent molecular phylogenetic analysis of lagenidium strains recovered from subcutaneous lesions in cats, dogs, and a human with lagenidiosis resolved into four clades; one of them was lagenidium giganteum, but three others were novel.	2017	26924580
a new furanocoumarin from the fruits of scaevola taccada and antifungal activity against pythium insidiosum.	a new coumarin, scataccanol (1) and 10 known compounds were isolated from the fruits of scaevola taccada (gaertn.) roxb. all compounds were evaluated for antifungal activity against pythium insidiosum. compounds 5 and 7 showed strong antifungal activity with minimum inhibitory concentration values of 5 and 10 μg/ml, respectively. structural determination of all compounds was accomplished by 1d and 2d-nmr, ir and ms.	2017	27223164
pythium insidiosum: inhibitory effects of propolis and geopropolis on hyphal growth.	propolis and geopropolis are resinous products of bees showing antimicrobial effects. there is no data concerning their action against pythium insidiosum - the causative agent of pythiosis, a pyogranulomatous disease of the subcutaneous tissue that affects mostly horses, dogs and humans. fragments of 15 isolates of p. insidiodum were incubated with propolis and geopropolis extracts and evaluated for up to seven days to detect the minimal fungicidal concentration (mfc). propolis inhibited three i ...	2017	27522931
extraction, characterization and biological activity of a (1,3)(1,6)-β-d-glucan from the pathogenic oomycete pythium insidiosum.	pythiosis is a life-threatening infectious disease caused by the pathogenic oomycete pythium insidiosum. this study is the first to evaluate the p. insidiosum glucan content and its biological activities. the enzymatic quantification of the glucans in p. insidiosum mycelia showed that the β-glucan content was 18.99%±3.59. the cell wall polysaccharide extract consisted of ∼81.7% carbohydrates (exclusively glucose) and ∼18.3% residual amino acids and peptides. the results from monosaccharide compo ...	2017	27987983
microevolutionary analyses of pythium insidiosum isolates of brazil and thailand based on exo-1,3-β-glucanase gene.	pythium insidiosum is an important oomycete due to its ability to infect humans and animals. it causes pythiosis, a disease of difficult treatment that occurs more frequently in humans in thailand and in horses in brazil. since cell-wall components are frequently related to host shifts, we decided here to use sequences from the exo-1,3-β-glucanase gene (exo1), which encodes an immunodominant protein putatively involved in cell wall remodeling, to investigate the microevolutionary relationships o ...	2017	27894990
development and evaluation of an elisa for the quantitation of anti-lagenidium giganteum forma caninum antibodies in dogs.	lagenidium giganteum forma caninum infection causes severe cutaneous and disseminated disease in dogs. currently, diagnosis requires culture and rrna gene sequencing.	2017	25274441