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risk analysis of the re-emergence of plasmodium vivax malaria in japan using a stochastic transmission model.this study analyzed the risk of infection with plasmodium vivax in local residents through a stochastic simulation in which an infected tourist, local resident, or immigrants from an endemic area would visit himi-shi, toyama prefecture, which is a formerly endemic area in japan.201121431796
seasonal prevalence of mosquitoes, including vectors of brugian filariasis, in southern islands of the republic of korea.a survey of mosquitoes, including the vector status of brugia malayi filariasis and their relative larval density, was conducted from 2002 to 2005 at several southern remote islands of jeollanam-do (province), gyeongsangnam-do, and jeju-do, korea, where filariasis was previously endemic. overall, a total of 9 species belonging to 7 genera were collected. ochlerotatus togoi (formerly known as aedes togoi), anopheles (hyrcanus) group, and culex pipiens were the predominant species captured at all ...201121461270
the dominant anopheles vectors of human malaria in the asia-pacific region: occurrence data, distribution maps and bionomic precis.abstract: background: the final article in a series of three publications examining the global distribution of 41 dominant vector species (dvs) of malaria is presented here. the first publication examined the dvs from the americas, with the second covering those species present in africa, europe and the middle east. here we discuss the 19 dvs of the asian-pacific region. this region experiences a high diversity of vector species, many occurring sympatrically, which, combined with the occurrence ...201121612587
emergence of genotype i of japanese encephalitis virus as the dominant genotype in asia.japanese encephalitis virus (jev), a mosquito-borne zoonotic pathogen, is one of the major causes of viral encephalitis worldwide. previous phylogenetic studies based on the envelope protein indicated that there are four genotypes, and surveillance data suggest that genotype i is gradually replacing genotype iii as the dominant strain. here we report an evolutionary analysis based on 98 full-length genome sequences of jev, including 67 new samples isolated from humans, pigs, mosquitoes, midges. ...201121697481
discovery of dna viruses in wild-caught mosquitoes using small rna high throughput sequencing.mosquito-borne infectious diseases pose a severe threat to public health in many areas of the world. current methods for pathogen detection and surveillance are usually dependent on prior knowledge of the etiologic agents involved. hence, efficient approaches are required for screening wild mosquito populations to detect known and unknown pathogens.201121949749
the abundance and host-seeking behavior of culicine species (diptera: culicidae) and anopheles sinensis in yongcheng city, people's republic of china.abstract: background: the knowledge of mosquito species diversity and the level of anthropophily exhibited by each species in a region are of great importance to the integrated vector control. culicine species are the primary vectors of japanese encephalitis (je) virus and filariasis in china. anopheles sinensis plays a major role in the maintenance of plasmodium vivax malaria transmission in china. the goal of this study was to compare the abundance and host-seeking behavior of culicine specie ...201122115320
Mosquito species composition and Plasmodium vivax infection rates on Baengnyeong-do (island), Republic of Korea.Vivax malaria is a significant military and civilian health threat in the north of the Republic of Korea (ROK). The island of Baengnyeong-do is the westernmost point of the ROK and is located close to the southwestern coast of the Democratic People's Republic of Korea (DPRK). Mosquitoes were collected using a black light trap on Baengnyeong-do, and Anopheles spp. were assayed by PCR, to identify the species, and screened for sporozoites of Plasmodium vivax. Of a subsample of 257 mosquitoes, Anop ...201122072836
the role of pvs28 in sporozoite development in anopheles sinensis and its longevity in balb/c mice.to develop a vivax malaria vaccine for blocking malarial transmission, the ookinete surface protein pvs28 was cloned from korean malaria patients using polymerase chain reaction. the pvs28 gene consists of 726bp and encodes 241 amino acids. it was subcloned into the expression vector pqe30 and expressed in escherichia coli. the expressed recombinant protein, rpvs28, has a molecular weight of about 28 kda in sds-page analysis. a monoclonal antibody against rpvs28 was produced using balb/c mice. i ...201120801117
molecular epidemiological investigation of plasmodium knowlesi in humans and macaques in singapore.singapore reported its first locally acquired human plasmodium knowlesi infection in 2007, involving a soldier who had undergone training in a forested area where long-tailed macaques are frequently seen. comprehensive disease surveillance and monitoring system that was set up after the initial case detected four additional human p. knowlesi cases in 2007 and one in 2008. all involved military personnel who had undergone training in the forested area, and none had traveled out of singapore 1 mon ...201120586605
nationwide surveillance of west nile virus targeting mosquitoes and dead birds from april 2004 through march 2007 in japan.we conducted nationwide west nile virus (wnv) surveillance targeting mosquitoes and dead birds to reveal whether the virus and its potential vectors are present in japan. a total of 12 766 mosquitoes and 230 dead birds were collected in april 2004-march 2005 (the 2004-2005 period), 10 755 mosquitoes and 267 dead birds in april 2005-march 2006 (the 2005-2006 period), and 8624 mosquitoes and 245 dead birds in april 2006-march 2007 (the 2006-2007 period). the species of most of the mosquitoes colle ...201120163573
population structure of the malaria vector anopheles sinensis (diptera: culicidae) in china: two gene pools inferred by microsatellites.anopheles sinensis is a competent malaria vector in china. an understanding of vector population structure is important to the vector-based malaria control programs. however, there is no adequate data of a. sinensis population genetics available yet.201121799796
distribution and larval habitats of anopheles species in northern gyeonggi province, republic of korea.a total of 180 larval collection sites (e.g., rice paddies, marshes, ground pools, ponds, stream margins, and irrigation and drainage ditches) was surveyed within a 2 km radius from warrior base training area, 5 km south of panmunjeom (joint security area, demilitarized zone), gyeonggi province, republic of korea (rok), from may through october, 2007 to characterize larval habitat distributions of members of the anopheles hyrcanus group (an. sinensis, an. lesteri, an. pullus, an. belenrae, an. k ...201121635650
[survey on malaria vectors in jiangsu province, 2005-2009].to understand the species, density and seasonal variation of malaria vectors in jiangsu province, so as to provide scientific evidence for malaria control.201122164867
[sensitivity of anopheles sinensis to insecticides in jiangsu province].to understand the sensitivity of anopheles sinensis to deltamethrin, ddt and malathion in jiangsu province.201122164497
molecular taxonomy of members of the anopheles hyrcanus group from thailand and indonesia.during studies of malaria vectors in indonesia and thailand, several specimens identified by field staff as members of the anopheles barbirostris group (diptera: culicidae) were found to belong to the anopheles hyrcanus group, as shown by marked differences in the size of the nuclear rdna second internal transcribed spacer (its2) between the barbirostris (~1500 bp) and hyrcanus (~600 bp) groups. identification of the species concerned required a more detailed study of its2 sequences and subunit ...201121198713
first report on co-occurrence knockdown resistance mutations and susceptibility to beta-cypermethrin in anopheles sinensis from jiangsu province, china.the increasing prevalence of insecticide resistance in anopheles sinensis, a major vector of malaria in jiangsu province in eastern china, threatens to compromise the successful use of insecticides in malaria control strategies. it is therefore vital to understand the insecticide resistance status of an. sinensis in the region. this study examined the nucleotide diversity of the para-sodium channel and knockdown resistance (kdr) in five field populations of adult an. sinensis mosquitoes collecte ...201222272229
comparative evaluation of light-trap catches, electric motor mosquito catches and human biting catches of anopheles in the three gorges reservoir.the mosquito sampling efficiency of light-trap catches and electric motor mosquito catches were compared with that of human biting catches in the three gorges reservoir. there was consistency in the sampling efficiency between light-trap catches and human biting catches for anopheles sinensis (r = 0.82, p<0.01) and light-trap catches were 1.52 (1.35-1.71) times that of human biting catches regardless of mosquito density (r = 0.33, p>0.01), while the correlation between electric motor mosquito ca ...201222235256
[investigation on the sensitivity of anopheles sinensis to insecticide].to investigate the sensitivity of anopheles sinensis to ddt, cyfluthrin, malathion and deltamethrin.201223072164
first detection of multiple knockdown resistance (kdr)-like mutations in voltage-gated sodium channel using three new genotyping methods in anopheles sinensis from guangxi province, china.to investigate knockdown resistance (kdr)-like mutations associated with pyrethroid resistance in anopheles sinensis (wiedemann, 1828), from guangxi province, southwest china, a segment of a sodium channel gene was sequenced and genotyped using three new genotyping assays. direct sequencing revealed the presence of ttg-to-tcg and tg-to-ttt mutations at allele position l1014, which led to l1014s and l1014f substitutions in a few individual and two novel substitutions of n1013s and l1014w in two d ...201223025181
[lymphatic filariasis in hubei province: from prevailing to elimination].lymphatic filariasis used to highly prevalent in 69 counties (cities) with 29 million people at the risk of infection in hubei province. there were 2 million people infected either by b. malayi or w. bancrofti that 1.6 million microfilaremia cases and 0.4 million chronic patients. the average rate of microfilaremia among population was 5.94%. anopheles sinensis and culex quinquefasciatus were the principal transmitting vectors. since 1970s, with the strategy of taking elimination of infection so ...201222913195
[effect of intermittent irrigation in paddy fields on mosquito control].to investigate the effects of intermittent irrigation in paddy fields on mosquito control and the impact on growth and yield of rice.201222590874
distribution of mosquitoes and mosquito-borne viruses along the china-myanmar border in yunnan province.a total of 54,673 mosquitoes were collected at 11 sites located near the china-myanmar border in the western part of yunnan province during july and august 2007. there were 29 species in 4 genera identified from the collections, including 12 species of culex, 12 species of anopheles, 3 species of aedes, and 2 species of armigeres. culex tritaeniorhynchus giles (67.9%, 37,119/54,673) and anopheles sinensis wiedemann (25.9%, 14,170/54,673) were the most abundant species in this investigation. viru ...201222627302
[genotype i japanese encephalitis virus is the main genotype in mosquito in fujian province].to grasp the infection rate and genotypes of japanese encephalitis virus (jev) in mosquito in fujian province.201223002538
malaria surveillance-response strategies in different transmission zones of the people's republic of china: preparing for climate change.a sound understanding of malaria transmission patterns in the people's republic of china (p.r. china) is crucial for designing effective surveillance-response strategies that can guide the national malaria elimination programme (nmep). using an established biology-driven model, it is expected that one may design and refine appropriate surveillance-response strategies for different transmission zones, which, in turn, assist the nmep in the ongoing implementation period (2010-2020) and, potentiall ...201223256579
dispersal range of anopheles sinensis in yongcheng city, china by mark-release-recapture methods.studying the dispersal range of anopheles sinensis is of major importance for understanding the transition from malaria control to elimination. however, no data are available regarding the dispersal range of an. sinensis in china. the aim of the present study was to study the dispersal range of an. sinensis and provide the scientific basis for the development of effective control measures for malaria elimination in china.201223226489
vector capacity of anopheles sinensis in malaria outbreak areas of central china.both falciparum and vivax malaria were historically prevalent in china with high incidence. with the control efforts, the annual incidence in the whole country has reduced to 0.0001% except in some areas in the southern borders after 2000. despite this, the re-emergence or outbreak of malaria was unavoidable in central china during 2005-2007. in order to understand the role of the vector in the transmission of malaria during the outbreak period, the vector capacity of an. sinensis in huanghuai v ...201222776520
spatial correlation between malaria cases and water-bodies in anopheles sinensis dominated areas of huang-huai plain, china.malaria re-emerged in the huang-huai plain of central china during 2006-2008, dominated with anopheles sinensis as a vector. however, there is no information on strategies based on multi-factor analysis to effectively control the re-emergence of malaria in these areas. previous experience indicates some relationship between the distribution of water bodies and malaria cases, but more detailed data are not available and in-depth studies have not been conducted up to now. the objective of this stu ...201222650153
genetic variation of aldolase from korean isolates of plasmodium vivax and its usefulness in serodiagnosis.the malaria aldolase is widely used as rapid diagnostic test (rdt), but the efficacy in aspect of its serological effectiveness in diagnosis is not known. the genetic variation of korean isolates was analysed and recombinant aldolase was evaluated as a serological antigen in plasmodium vivax malaria.201222569198
population structure and transmission dynamics of plasmodium vivax in the republic of korea based on microsatellite dna analysis.in order to control malaria, it is important to understand the genetic structure of the parasites in each endemic area. plasmodium vivax is widely distributed in the tropical to temperate regions of asia and south america, but effective strategies for its elimination have yet to be designed. in south korea, for example, indigenous vivax malaria was eliminated by the late 1970s, but re-emerged from 1993. we estimated the population structure and temporal dynamics of transmission of p. vivax in so ...201222509416
[establishment of early warning system of malaria epidemic situation in jiangsu province i impact of temperature on development of plasmodium vivax in anopheles sinensis in jiangsu province].to observe the developmental threshold temperature and the effective accumulated temperature of plasmodium vivax sporozoites in anopheles sinensis in jiangsu province, and to analyze the impact of temperature on the development of plasmodium vivax.201223373272
[cloning and sequence analysis of cdna encoding aquaporin (aqp) gene from anopheles sinensis].to clone and analyze the full-length sequence of aquaporin gene of anopheles sinensis (asaqp), so as to provide an insight into its biology functions.201223593837
spatial patterns of malaria reported deaths in yunnan province, china.malaria has been a heavy social and health burden in the remote and poor areas in southern china. analyses of malaria epidemic patterns can uncover important features of malaria transmission. this study identified spatial clusters, seasonal patterns, and geographic variations of malaria deaths at a county level in yunnan, china, during 1991-2010. a discrete poisson model was used to identify purely spatial clusters of malaria deaths. logistic regression analysis was performed to detect changes i ...201223269660
[resistance of anopheles sinensis to insecticides in huaibin county of henan province].anopheles sinensis adults were captured from huaibin county and raised in laboratory. larvae dipping method and the who tubes were used to determine the resistance of f1 iv-stage larvae and 3-5 day-old adult mosquitoes to dimethyl dichloroviny phosphate (ddvp), deltamethrin, and arprocarb. the activity of acetylcholine esterase (ache) of larvae and adult mosquitoes was measured by colorimetric method before and after the respective addition of the 3 insecticides. the results showed that the mort ...201223484266
japanese encephalitis virus in mosquitoes and swine in yunnan province, china 2009-2010.the residential regions of yunnan province, canton of jing hong, in china were surveyed for japanese encephalitis virus (jev) infection in mosquito and swine vectors to determine the frequency of jev-carrying zoonotic vectors in 2009-2010. a total of 21,500 mosquitoes were collected and divided by species, and brain tissue was collected from 108 stillborn piglets. the infection rates for the different jev species were 13.2% for culex tritaeniorhynchus, 2.7% for anopheles sinensis, 0.7% for armig ...201223199264
isolation and identification of a distinct strain of culex flavivirus from mosquitoes collected in mainland china.culexflavivirus (cxfv) is an insect specific virus that has been isolated from culexpipiens, culexquinquefasciatus, culextritaeniorhynchus and other culex mosquitoes. it is a novel flavivirus isolated in asia, north america, central america and africa. phylogenetic analysis indicates that, based on the envelope gene (e gene) sequence, the worldwide cxfv strains can be divided into two genotypes.201222452813
mosquito species composition and plasmodium vivax infection rates for korean army bases near the demilitarized zone in the republic of korea, 2011.vivax malaria is a significant military and civilian health threat in northern republic of korea (rok). mosquito collections were performed at two rok army installations, paju near the demilitarized zone (dmz) using black light traps in 2011. the dmz, a 4 km wide border, is the northernmost point of the rok and separates the rok from the democratic people's republic of korea (dprk). anopheles spp. were identified by polymerase chain reaction and screened for plasmodium vivax sporozoites. of 4,35 ...201223243112
the polymorphism and the geographical distribution of the knockdown resistance (kdr) of anopheles sinensis in the republic of korea.in the republic of korea (rok), six sibling species of the anopheles sinensis complex are considered the vector species of malaria, but data on their susceptibilities to malaria and vector capacities have been controversial. the intensive use of insecticides has contributed to the rapid development and spread of insecticide resistance in the an. sinensis complex. knockdown resistance (kdr) to pyrethroids and ddt in the an. sinensis complex is associated with a mutation in codon 1014 of the volta ...201222554130
random repeated cross sectional study on breeding site characterization of anopheles sinensis larvae in distinct villages of yongcheng city, people's republic of china.characterizing the breeding site of anopheles sinensis is of major importance for the transition from malaria control to elimination in china. however, little information is available especially regarding the characteristics and influencing factors of breeding sites of an. sinensis in yongcheng city, a representative region of unstable malaria transmission in the huang-huai river region of central china. the aims of this study were to determine the breeding site characteristics of an. sinensis a ...201222444032
identifying the main mosquito species in china based on dna barcoding.mosquitoes are insects of the diptera, nematocera, and culicidae families, some species of which are important disease vectors. identifying mosquito species based on morphological characteristics is difficult, particularly the identification of specimens collected in the field as part of disease surveillance programs. because of this difficulty, we constructed dna barcodes of the cytochrome c oxidase subunit 1, the coi gene, for the more common mosquito species in china, including the major dise ...201223071708
estimating the malaria transmission of plasmodium vivax based on serodiagnosis.plasmodium vivax re-emerged in 1993 and has now become a major public health problem during the summer season in south korea. the aim of this study was to interpret and understand the meaning of seroepidemiological studies for developing the best malaria control programme in south korea.201222852558
comparative transcriptome analyses of deltamethrin-resistant and -susceptible anopheles gambiae mosquitoes from kenya by rna-seq.malaria causes more than 300 million clinical cases and 665,000 deaths each year, and the majority of the mortality and morbidity occurs in sub-saharan africa. due to the lack of effective vaccines and wide-spread resistance to antimalarial drugs, mosquito control is the primary method of malaria prevention and control. currently, malaria vector control relies on the use of insecticides, primarily pyrethroids. the extensive use of insecticides has imposed strong selection pressures for resistanc ...201222970263
maiden outbreak of chikungunya in dongguan city, guangdong province, china: epidemiological characteristics.this study was conducted to identify epidemiological characteristics of the first documented chik fever outbreak in china and evaluate the effect of the preventive measures taken.201222916166
frontiers of parasitology research in the people's republic of china: infection, diagnosis, protection and surveillance.control and eventual elimination of human parasitic diseases in the people's republic of china (p.r. china) requires novel approaches, particularly in the areas of diagnostics, mathematical modelling, monitoring, evaluation, surveillance and public health response. a comprehensive effort, involving the collaboration of 188 scientists (>85% from p.r. china) from 48 different institutions and universities (80% from p.r. china), covers this collection of 29 articles published in parasites & vectors ...201223036110
change in rainfall drives malaria re-emergence in anhui province, china.malaria is re-emerging in anhui province, china after a decade long' low level of endemicity. the number of human cases has increased rapidly since 2000 and reached its peak in 2006. that year, the malaria cases accounted for 54.5% of total cases in mainland china. however, the spatial and temporal patterns of human cases and factors underlying the re-emergence remain unclear. we established a database containing 20 years' (1990-2009) records of monthly reported malaria cases and meteorological ...201222928015
the history of the elimination of lymphatic filariasis in china.china used to be one of the most heavily endemic countries for lymphatic filariasis (lf) in the world. there were 864 endemic counties/cities in 16 provinces/autonomous regions/municipalities (p/a/m) with a total population of 330 million at risk of infection. since the founding of the people's republic of china in 1949, the chinese government has designated the control of the disease to be a top priority. due to decades of sustained efforts, close cooperation related to lf control among governm ...201324289733
blood stage of plasmodium vivax in central china is still susceptible to chloroquine plus primaquine combination therapy.in central china, plasmodium vivax accounts for all of the native reported cases of malaria. chloroquine (cq) plus primaquine (pq) have been used for more than 60 years as the frontline drugs, but the risk of treatment failure remains unknown. to measure the effectiveness and safety of cq-pq among vivax malaria patients, a total of 39 subjects with monoinfection vivax malaria was enrolled in a study from 2008 to 2009. there were no recrudescence or danger signs observed within the 28-day follow- ...201323669232
the long and short incubation periods of plasmodium vivax malaria in korea: the characteristics and relating factors.the cases of plasmodium vivax malaria in korea are mixed with long and short incubation periods. this study aims to define clinico-epidemiologic chracteristcs of plasmodium vivax malaria in korea.201324265966
application of structural equation models for elucidating the ecological drivers of anopheles sinensis in the three gorges reservoir.to identify the major ecological drivers for malaria vector density using the structural equation model (sem) in the three gorges reservoir.201323935887
genetic variations of nd5 gene of mtdna in populations of anopheles sinensis (diptera: culicidae) malaria vector in china.anopheles sinensis is a principal vector for plasmodium vivax malaria in most parts of china. understanding of genetic structure and genetic differentiation of the mosquito should contribute to the vector control and malaria elimination in china.201324192424
microsatellite dna analysis revealed a drastic genetic change of plasmodium vivax population in the republic of korea during 2002 and 2003.vivax malaria was successfully eliminated in the republic of korea (south korea) in the late 1970s, but it was found to have re-emerged from 1993. in order to control malaria and evaluate the effectiveness of malaria controls, it is important to develop a spatiotemporal understanding of the genetic structure of the parasite population. here, we estimated the population structure and temporal dynamics of the transmission of plasmodium vivax in south korea by analyzing microsatellite dna markers o ...201324205429
susceptibility of anopheles sinensis to plasmodium vivax in malarial outbreak areas of central china.anopheles sinensis, anopheles anthropophagus, anopheles minimus and anopheles dirus are the major vectors of malaria transmission in china. anopheles sinensis is considered a secondary vector due to its relatively low malaria-transmission ability. however, in 2005, an outbreak of over 40,000 plasmodium vivax malaria cases was reported in areas where anopheles sinensis was the only major vector. therefore, it is necessary to reassess the malaria transmission ability of this vector species in chin ...201323768077
the susceptibility of aedes aegypti populations displaying temephos resistance to bacillus thuringiensis israelensis: a basis for management.aedes aegypti is the vector of dengue virus, and its control is essential to prevent disease transmission. among the agents available to control this species, biolarvicides based on bacillus thuringiensis serovar israelensis (bti) are an effective alternative to replace the organophosphate temephos for controlling populations that display resistance to this insecticide. the major goal of this study was to determine the baseline susceptibility of brazilian ae. aegypti populations to bti, taking i ...201324499507
decreasing incidence of plasmodium vivax in the republic of korea during 2010-2012.after the re-emergence of plasmodium vivax in 1993, a total of 31,254 cases of vivax malaria were reported between 1993-2012 in the republic of korea (rok). the purpose of this study was to review korea centers for disease control and prevention records to investigate the transmission of malaria from 2010-2012.201324010412
a potential threat to malaria elimination: extensive deltamethrin and ddt resistance to anopheles sinensis from the malaria-endemic areas in china.insecticide resistance in malaria vectors is a growing concern in many countries and requires immediate attention because of the limited chemical arsenal available for vector control. there is lack of systematic and standard monitoring data of malaria vector resistance in the endemic areas, which is essential for the ambitious goal of malaria elimination programme of china.201323683359
entomological determinants of insecticide-treated bed net effectiveness in western myanmar.in a large cluster randomized control trial of insecticide-treated bed nets (itn) in western myanmar the malaria protective effect of itn was found to be highly variable and, in aggregate, the effect was not statistically significant. a coincident entomological investigation measured malaria vector abundance and biting behaviour and the human population sleeping habits, factors relevant to itn effectiveness.201324119994
the effects of climate change and globalization on mosquito vectors: evidence from jeju island, south korea on the potential for asian tiger mosquito (aedes albopictus) influxes and survival from vietnam rather than japan.climate change affects the survival and transmission of arthropod vectors as well as the development rates of vector-borne pathogens. increased international travel is also an important factor in the spread of vector-borne diseases (vbds) such as dengue, west nile, yellow fever, chikungunya, and malaria. dengue is the most important vector-borne viral disease. an estimated 2.5 billion people are at risk of infection in the world and there are approximately 50 million dengue infections and an est ...201323894312
a video clip of the biting midge culicoides anophelis ingesting blood from an engorged anopheles mosquito in hainan, china.biting midges are hematophagus ectoparasites of insects, humans and other animals. culicoides (trithicoides) anophelis edwards1922 is a predator of engorged mosquitoes.201324499575
novel virus discovery and genome reconstruction from field rna samples reveals highly divergent viruses in dipteran hosts.we investigated whether small rna (srna) sequenced from field-collected mosquitoes and chironomids (diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using srnas sequenced from wild-caught specimens, to inform total rna deep sequencing of samples of particular interest. using this strategy, we sequenced from adult anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chro ...201324260463
mosquitoes of western yunnan province, china: seasonal abundance, diversity, and arbovirus associations.the western borderland between yunnan province, china, and myanmar is characterized by a climate that facilitates year-round production of mosquitoes. numerous mosquito-transmitted viruses, including japanese encephalitis virus circulate in this area. this project was to describe seasonal patterns in mosquito species abundance and arbovirus activity in the mosquito populations.201324146951
epidemiological characteristics of malaria in ningbo city, china 2000-2011.the objective of this study was to explore the trend and the epidemiological characteristics of malaria in ningbo from 2000 through 2011 and provide scientific evidence for the prevention and control. using the retrospective study, data relating to malaria epidemics during 2000-2011 in ningbo were collected through the questionnaires of the cases and other important surveillance data. adult mosquitoes were collected using cdc light traps baited with co2. results showed that plasmodium vivax was ...201323959492
presence of entomobirnaviruses in chinese mosquitoes in the absence of dengue virus co-infection.birnaviruses, including the genus entomobirnavirus, are socio-economically important viruses. currently, only drosophila x virus has been formally assigned to the genus entomobirnavirus, but two more viruses were recently isolated, espirito santo virus (esv) and culex y virus. the host mosquito has been reported to carry many viruses, but seldom entomobirnaviruses. to discover potential pathogens in mosquitoes, we exploited small-rnas high-throughput sequencing of three mosquito species caught i ...201323175239
larvicidal, oviposition, and ovicidal effects of artemisia annua (asterales: asteraceae) against aedes aegypti, anopheles sinensis, and culex quinquefasciatus (diptera: culicidae).this study focuses on the larvicidal, oviposition, and ovicidal effects of a crude extract of artemisia annua against aedes aegypti, anopheles sinensis, and culex quinquefasciatus. dried cells of artemisia annua from cell suspension cultures were extracted using hexane. the extract showed moderate larvicidal effects against mosquitoes. at 24-h post treatment, the lc50 values for anopheles sinensis, aedes aegypti, and culex quinquefasciatus were recorded as 244.55, 276.14, and 374.99 ppm, respect ...201323835922
dna barcoding for the identification of eight species members of the thai hyrcanus group and investigation of their stenogamous behavior.eight species members of the thai hyrcanus group were identified based on the intact morphology and molecular analysis (coi barcoding, 658 bp) of f1-progenies. five iso-female lines of each species were pooled in order to establish stock colonies. a stenogamous colony of each species was investigated by making 200 and 300 newly emerged adult females and males co-habit in a 30 cm cubic cage for one week. after ovipositon, the spermathecae of females were examined for sperms. the results revealed ...201324161242
mosquitoes and other aquatic insects in fallow field biotopes and rice paddy fields.fallow field biotopes that develop from abandoned rice fields are man-made wetlands that provide new habitats for various aquatic animals. although consideration of such biotopes generally focuses on their positive aspects, this study evaluated the negative aspects of establishing fallow field biotopes with regard to mosquito breeding sites. to determine whether fallow field biotopes become breeding habitats for vector mosquitoes, we evaluated mosquito fauna in fallow field biotopes and adjacent ...201323167444
relationship between knockdown resistance, metabolic detoxification and organismal resistance to pyrethroids in anopheles sinensis.anopheles sinensis is the most important vector of malaria in southeast asia, including china. currently, the most effective measure to prevent malaria transmission relies on vector control through the use of insecticides, primarily pyrethroids. extensive use of insecticides poses strong selection pressure on mosquito populations for resistance. resistance to insecticides can arise due to mutations in the insecticide target site (target site resistance), which in the case of pyrethroids is the p ...201323405157
[taxonomic composition of metagenomic community in the larval gut of mosquito anopheles sinensis (diptera: culicidae)].to investigate the bacteria diversity in larval gut of field-collected anopheles sinensis.201324809191
[deltamethrin resistance, metabolic detoxification enzyme and kdr mutation in anopheles sinensis in region along huaihe river in anhui province].to understand the deltamethrin resistance level, gts and p450 metabolic detoxification enzyme activities, and mutations in the para-type sodium channel gene that confers knockdown resistance (kdr) in anopheles sinensis mosquitoes from malaria endemic areas along the huaihe river in anhui province, china.201324024445
[study on taqman-mgb real-time fluorescence quantitative pcr to detect gene mutation of kdr from anopheles sinensis].to establish a real-time fluorescence quantitative pcr to detect the kdr gene mutation in anopheles sinensis.201323894838
[changes of pyrethroid resistance and p450 monooxygenase activity with age in anopheles sinensis in huainan city, anhui province, china].to study the effect of mosquito age on the pyrethroid resistance and p450 monooxygenase activity in anopheles sinensis in huainan city, anhui province, china, as well as the molecular mechanism of pyrethroid resistance.201323894836
[malaria surveillance in shanghai from 2005 to 2012].to understand the status of malaria prevalence in shanghai, so as to provide the evidence for evaluating and promoting malaria elimination.201424800562
[resistance of anopheles sinensis to three common insecticides in hainan province].anopheles sinensis adults were collected by cow-baited trap in haikou city, sanya city, lingshui county, changjiang county and qiongzhong county of hainan province during 2011-2013. the mosquitoes were exposed to insecticide impregnated papers with discriminating concentrations of ddt (4%), deltamethrin (0.05%), and malathion (5%) using who standard assays. knockdown rate was recorded at 10, 15, 20, 30, 40, 50, and 60 min, and kt50 values were calculated. mortality was recorded after 24 hours of ...201425065212
[identification of mammalian blood meals in anopheline mosquitoes from four counties of yunnan province by multiple pcr].from june to august 2012, the blood-sucking mosquitoes were captured around cattle-sheds and human houses in yuanjiang county, qiaojia county, yongshan county, and jinghong city of yunan province. blood samples from mosquitoes were collected on filter paper. multiplex pcr assay was used to detect the blood meal samples. among the 145 mosquitoes captured, 123 were anopheles sinensis (84.8%) and 22 a. minimus (15.2%). among the blood samples, corresponding bands were amplified in 134 samples. the ...201424822373
[surveillance of malaria in huai'an district, huai'an city, 2009-2013].to analyze the results of malaria surveillance in huai' an district in the recent 5 years, so as to provide the evidences for the establishment of suitable control strategy in the district.201425434159
application of auto-regressive linear model in understanding the effect of climate on malaria vectors dynamics in the three gorges reservoir.it is important to understand the dynamics of malaria vectors in implementing malaria control strategies. six villages were selected from different sections in the three gorges reservoir for exploring the relationship between the climatic factors and its malaria vector density from 1997 to 2007 using the auto-regressive linear model regression method. the result indicated that both temperature and precipitation were better modeled as quadratic rather than linearly related to the density of anoph ...201425341817
[sequence analysis of ribosomal dna internal transcribed spacer 2 of sympatric populations of anopheles sinensis of different feeding preferences].to investigate the existence of genetic divergence of sympatric populations of anopheles sinensis of different feeding preferences based on the rdna-its2 sequence differences.201425782250
influence of substrate color on oviposition behavior, egg hatchability, and substance of egg origin in the mosquito anopheles sinensis (wiedemann) (diptera: culicidae).understanding the factors that influence the choice of substrate for oviposition by the malaria mosquito is critical to efforts directed to the management of the disease and vector control measures aimed at modifications of larval habitat. the influence of black and white substrates on anopheles sinensis (wiedemann) (culicidae: anophelinae) female oviposition behavior and egg-hatching rate and the presence of substances associated with egg laying were studied. results from the no-choice tests sh ...201427193959
[surveillance of malaria in huai'an district, huai'an city, 2009-2013].to analyze the results of malaria surveillance in huai' an district in the recent 5 years, so as to provide the evidences for the establishment of suitable control strategy in the district.201425507757
measure post-bloodmeal dispersal of mosquitoes and duration of radioactivity by using the isotope ³²p.the radioactive isotope (32)p-labeled disodium phosphate (na₂h(32)po₄) was injected via the jugular vein into a cow kept in a shed in maozhuang village, cao township of shanxian county, china. over the following 5 d, mosquitoes feeding on the cow were captured at distances up to 400 m to determine dispersal distance. the duration of radioactivity in the cow and marked mosquitoes was 10 d. the results showed that after blood feeding, anopheles sinensis and culex tritaeniorhynchus temporarily rest ...201425502034
scanning electron microscopy of anopheles hyrcanus group (diptera: culicidae) eggs in thailand and an ultrastructural key for species identification.the eggs of anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae, anopheles peditaeniatus, anopheles pursati, and anopheles sinensis are described with the aid of scanning electron micrographs. comparisons of the egg structure among the eight species showed that the eggs differed with respect to the following characteristics: the deck-complete (an. argyropus, an. nigerrimus, an. paraliae, an. peditaeniatus, and an. sinensis); variable (complete, s ...201424337511
[experimental observation on host preference of anopheles sinensis].to observe the host preference of anopheles sinensis captured by outdoor human or cattle baits.201425902680
colonization and maintenance of anopheles kleini and anopheles sinensis from the republic of korea.colonization of members of the anopheles hyrcanus group is required to facilitate a better understanding of their roles in the epidemiology, vector competence, transmission potential, and maintenance of vivax malaria in the republic of korea (rok). a total of 158 resting blood-fed wild caught anopheles species were collected on august 26, 2011 at tongilchon, near the demilitarized zone, in the rok and shipped on august 28, 2011 to the entomology department, armed forces research institute of med ...201424772671
[investigation on mosquitoes and mosquito-borne viruses in dehong prefecture, yunnan province, 2007 and 2010].to investigate the distribution patterns of mosquito and mosquito-borne viruses in dehong prefecture, yunnan province, china.201425059361
malaria outbreaks in china (1990-2013): a systematic review.china has already achieved remarkable accomplishments in shrinking the malaria burden since the mid-20th century. the country now plans to eliminate malaria by the year 2020. looking at the dynamics of malaria outbreaks during the last decades might provide important information regarding the potential challenges of such an elimination strategy and might help to avoid mistakes of the past.201425012078
malaria in overseas labourers returning to china: an analysis of imported malaria in jiangsu province, 2001-2011.while great success in malaria control has been achieved in china, imported malaria has become a major challenge in the context of malaria elimination. this retrospective study describes the epidemiological profile of imported malaria and identifies the at-risk population during the period of 2001-2011 in jiangsu province.201424460982
genetic diversity of plasmodium vivax population in anhui province of china.although the numbers of malaria cases in china have been declining in recent years, outbreaks of plasmodium vivax malaria were still being reported in rural areas south of the yellow river. to better understand the transmission dynamics of p. vivax parasites in china, the extent of genetic diversity of p. vivax populations circulating in bozhou of anhui province of china were investigated using three polymorphic genetic markers: merozoite surface proteins 1 and 3α (pvmsp-1 and pvmsp-3α) and circ ...201424401153
genome sequence of anopheles sinensis provides insight into genetics basis of mosquito competence for malaria parasites.anopheles sinensis is an important mosquito vector of plasmodium vivax, which is the most frequent and widely distributed cause of recurring malaria throughout asia, and particularly in china, korea, and japan.201424438588
detection of quang binh virus from mosquitoes in china.flaviviruses present a wide range of genetic diversity and exhibit diverse host relationships. mosquito-borne flaviviruses have recently been isolated and characterized worldwide. yunnan province of china is one of the richest areas of species diversity and is the center of multi-species evolution in mainland asia, which supports the circulation of numerous arthropod-borne viruses (arboviruses). in a screening program of arboviruses, mosquitoes were collected during the mosquito activity season ...201424342141
host feeding patterns of mosquitoes in a rural malaria-endemic region in hainan island, china.malaria is endemic in wangxia village of hainan island. in this area little is known about the host seeking behavior and feeding habit of mosquitoes. three sites representing the most common habitat types in the village were selected to study the host seeking behavior and feeding habit of mosquitoes. of the total 9 species belonging to 4 genera (armigeres, culex, aedes, and anopheles) collected in wangxia village, culex tritaeniorhynchus and cx. pipiens quinquefasciatus were the most commonly co ...201425843138
comparison of genotypes i and iii in japanese encephalitis virus reveals distinct differences in their genetic and host diversity.japanese encephalitis (je) is an arthropod-borne disease associated with the majority of viral encephalitis cases in the asia-pacific region. the causative agent, japanese encephalitis virus (jev), has been phylogenetically divided into five genotypes. recent surveillance data indicate that genotype i (gi) is gradually replacing genotype iii (giii) as the dominant genotype. to investigate the mechanism behind the genotype shift and the potential consequences in terms of vaccine efficacy, human c ...201425056890
species composition, seasonal occurrence, habitat preference and altitudinal distribution of malaria and other disease vectors in eastern nepal.it is increasingly recognized that climate change can alter the geographical distribution of vector-borne diseases (vbds) with shifts of disease vectors to higher altitudes and latitudes. in particular, an increasing risk of malaria and dengue fever epidemics in tropical highlands and temperate regions has been predicted in different climate change scenarios. the aim of this paper is to expand the current knowledge on the seasonal occurrence and altitudinal distribution of malaria and other dise ...201425430654
mosquito surveillance revealed lagged effects of mosquito abundance on mosquito-borne disease transmission: a retrospective study in zhejiang, china.mosquito-borne diseases (mbds) are still threats to public health in zhejiang. in this study, the associations between the time-lagged mosquito capture data and mbds incidence over five years were used to examine the potential effects of mosquito abundance on patterns of mbds epidemiology in zhejiang during 2008-2012. light traps were used to collect adult mosquitoes at 11 cities. correlation tests with and without time lag were performed to investigate the correlations between mbds incidence ra ...201425393834
molecular epidemiology of japanese encephalitis virus in mosquitoes in taiwan during 2005-2012.japanese encephalitis (je) is a mosquito-borne zoonotic disease caused by the japanese encephalitis virus (jev). pigs and water birds are the main amplifying and maintenance hosts of the virus. in this study, we conducted a jev survey in mosquitoes captured in pig farms and water bird wetland habitats in taiwan during 2005 to 2012. a total of 102,633 mosquitoes were collected. culex tritaeniorhynchus was the most common mosquito species found in the pig farms and wetlands. among the 26 mosquito ...201425275652
molecular epidemiology of japanese encephalitis virus in mosquitoes during an outbreak in china, 2013.japanese encephalitis virus (jev) can cause serious encephalitis and culex mosquitoes are the primary vector. in 2013, a je outbreak occurred in shandong province, china with 407 confirmed cases, including 11 deaths. an investigation on jev in mosquitoes during the outbreak was conducted. a total of 14,719 mosquitoes were collected at 3 sites. for the 12,695 culex tritaeniorhynchus mosquitoes, 88/201 pooled samples were positive by rt-pcr for the presence of the pre-membrane or envelope protein ...201424809635
evidence to support natural hybridization between anopheles sinensis and anopheles kleini (diptera: culicidae): possibly a significant mechanism for gene introgression in sympatric populations.malaria caused by plasmodium vivax is still a public health problem in the republic of korea (rok), particularly regarding the recent re-emergence of this malarial species near the demilitarized zone in northwestern paju city, gyeonggi-do province. currently, at least 4 species (an. kleini, an. pullus, an. belenrae and an. lesteri) of the hyrcanus group are reported as possible natural vectors of vivax malaria in the rok, and an. sinensis, which is the most dominant species, has long been incrim ...201424443885
a household randomized, controlled trial of the efficacy of 0.03% transfluthrin coils alone and in combination with long-lasting insecticidal nets on the incidence of plasmodium falciparum and plasmodium vivax malaria in western yunnan province, china.mosquito coils are the most commonly used household insecticidal product in the world with sales exceeding 50 billion coils, used by two billion people worldwide annually. despite strong evidence that coils prevent mosquito bites a systematic review concluded that there is no evidence that burning mosquito coils prevents malaria acquisition. therefore, the current trial was designed to measure and compare prevention of malaria infection by mosquito coils or long-lasting insecticidal net (llin) o ...201424885993
sodium channel point mutations associated with pyrethroid resistance in chinese strains of culex pipiens quinquefasciatus (diptera: culicidae).pesticide resistance due to sodium channel point mutations has been well documented in many mosquito species.201425128988
dna barcoding: complementing morphological identification of mosquito species in singapore.taxonomy that utilizes morphological characteristics has been the gold standard method to identify mosquito species. however, morphological identification is challenging when the expertise is limited and external characters are damaged because of improper specimen handling. therefore, we explored the applicability of mitochondrial cytochrome c oxidase subunit 1 (coi) gene-based dna barcoding as an alternative tool to identify mosquito species. in the present study, we compared the morphological ...201425498759
mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids - a review.constant and extensive use of chemical insecticides has created a selection pressure and favored resistance development in many insect species worldwide. one of the most important pyrethroid resistance mechanisms is classified as target site insensitivity, due to conformational changes in the target site that impair a proper binding of the insecticide molecule. the voltage-gated sodium channel (nav) is the target of pyrethroids and ddt insecticides, used to control insects of medical, agricultur ...201425292318
molecular surveillance of pvdhfr, pvdhps, and pvmdr-1 mutations in plasmodium vivax isolates from yunnan and anhui provinces of china.plasmodium vivax is the predominant species of human malaria parasites present in china. although sulphadoxine-pyrimethamine (sp) and chloroquine (cq) have been widely used for malaria treatment in china, the resistance profiles of these drugs are not available. analysis of dihydrofolate reductase (dhfr), dihydropteroate synthase (dhps), and multidrug resistance (mdr-1) gene mutations in p. vivax isolates is a valuable molecular approach for mapping resistance to sp and cq. this study investigat ...201425179752
discovery and evolution of bunyavirids in arctic phantom midges and ancient bunyavirid-like sequences in insect genomes.bunyaviridae is a large family of rna viruses chiefly comprised of vertebrate and plant pathogens. we discovered novel bunyavirids that are approximately equally divergent from each of the five known genera. we characterized novel genome sequences for two bunyavirids, namely, kigluaik phantom virus (kigv), from tundra-native phantom midges (chaoborus), and nome phantom virus (nomv), from tundra-invading phantom midges, and demonstrated that these bunyavirid-like sequences belong to an infectious ...201424850747
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