Publications

[biological activities of chemically-treated glycolipids from salmonella minnesota re-mutant].		1985	4057552
biological activities of lipopolysaccharide fractionated by preparative acrylamide gel electrophoresis.	lipopolysaccharide from a smooth strain of salmonella minnesota was fractionated into two major fractions and one intermediate fraction by using sodium dodecylsulfate-polyacrylamide gel electrophoresis. on the basis of the study by hitchcock and brown, it was deduced that the top fraction was mainly long o-side chain lps and the bottom fraction was o-side chain-less lps. the middle fraction was a mixture of both short o-side chain lps and o-side chain-less lps. the antigenic properties and biolo ...	1985	3872989
relationship between immune system and gram-negative bacteria. iv. t lymphocytes from lpsd mice possess binding site(s) for rb salmonella.	we have previously shown that salmonella minnesota r345 (rb) spontaneously binds to 50 to 55% of human peripheral blood mononuclear cells (pbmc). in the present study, we have compared rb cytoadherence to lymphoid cells from various tissues of lipopolysaccharide (lps) hyporesponsive (lpsd) and lps responsive (lpsn) mouse strains. a higher number of spleen cells from lpsd mice (c3h/hej and c57bl/10scn) bound rb bacteria (22 to 30%) than cells from lpsn mice (4 to 9%). rb bound mainly to t cells, ...	1985	3876386
high-molecular-weight components in lipopolysaccharides of salmonella typhimurium, salmonella minnesota, and escherichia coli.	lipopolysaccharide from smooth strains of salmonella typhimurium, salmonella minnesota, and escherichia coli o111:b4, o55:b5, and o127:b8 was fractionated by gel filtration chromatography. all lipopolysaccharide samples separated into three major populations. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the fractions from s. typhimurium and s. minnesota indicated that the three peaks were made up of molecules with average o-antigen lengths of (i) 70 or more repeat units, (ii) 30 ...	1985	3886631
selective detection of 3-deoxymannooctulosonic acid in intact lipopolysaccharides by spin-echo 13c nmr.	the 3-deoxy-d-mannooctulosonic acid (kdo) region of lipopolysaccharides (lps) from the heptoseless mutant salmonella minnesota r595 and inner core and heptoseless mutants derived from escherichia coli k12 was studied by 13c nmr spectroscopy. a spin-echo spectral editing technique was employed for the selective detection of the quaternary anomeric carbon of ketosidically linked kdo. only two quaternary carbon resonances attributable to kdo were detected in the anomeric carbon spectral region of e ...	1985	3902810
isolation and partial characterization of the extracellular polysaccharides and lipopolysaccharides from fast-growing rhizobium japonicum usda 205 and its nod- mutant, hc205, which lacks the symbiotic plasmid.	the extracellular polysaccharides and lipopolysaccharides (lpss) from two fast-growing rhizobium japonicum strains, usda 205 and hc205, were isolated and partially characterized. strain hc205 is a nod- mutant of usda 205 which lacks the symbiotic plasmid. the extracellular polysaccharides from both strains are very similar in composition, having galactose, glucose, glucuronic acid, and acyl groups. the extracellular polysaccharides do not contain detectable levels of pyruvate. methylation analys ...	1985	4091554
relationship between immune system and gram-negative bacteria. iii. functional analysis of human peripheral blood lymphocyte subpopulations separated by cytoadherence with salmonella minnesota rb.	spontaneous adherence of bacteria to human peripheral blood mononuclear cells (pbmc) represents a useful tool for analysis of lymphocyte subsets with different functions. we have recently shown that pbmc can be divided into 2 populations based on their ability to bind salmonella minnesota r345 (rb) bacteria. by using these procedures, here, we provide evidence that rb-bound and rb-unbound pbmc populations give similar proliferative responses to phytohemagglutinin (pha) and concanavalin a (con a) ...	1985	2935333
toxicity of d-galactosamine for rat hepatocytes in monolayer culture.	hepatocellular injury was induced by exposure of primary cultures of rat hepatocytes to 4 mm d-galactosamine. the cell damage was very similar to that seen in vivo and in the isolated perfused rat liver, both in biochemical and in structural terms. the severity of the lesions caused by d-galactosamine was dependent on the age of the culture being treated. less severe damage was found with older cultures. since the primary metabolic effects of d-galactosamine were age-independent, the reduction i ...	1985	2857129
specific binding of endotoxin to human monocytes and mouse macrophages: serum requirement.	specific binding of bordetella pertussis and neisseria meningitidis endotoxins to human monocytes and murine macrophages was demonstrated. binding of b. pertussis endotoxin could be inhibited by endotoxins of salmonella minnesota, escherichia coli, and klebsiella pneumoniae, the extent of inhibition being dependent on the origin of the lipopolysaccharides and on the origin of the mononuclear phagocytic cells. the binding of b. pertussis and n. meningitidis endotoxins which was mediated by the po ...	1985	2857597
immunomodulation by bordetella pertussis: antiviral effects.	treatment of mice by intraperitoneal inoculation of pertussis vaccine or lipopolysaccharide extracted from b. pertussis will effect resistance to rabies virus, encephalomyocarditis virus, semliki forest virus, and herpes simplex virus. our previous observations indicated that treatment of c3h/hen (+/nu) and bdf1 mice with pertussis vaccine injected i.p. five days prior to a mouse adenovirus lethal dose i.p. challenge elicited resistance to clinical disease and death. susceptibility returned to a ...	1985	2872109
differences in attachment and phagocytosis of salmonella minnesota strains (s form, re mutant) by mouse peritoneal macrophages: participation of endogenous c1q and bacterial surface components (lps, porins).		1985	2417782
activation of murine spleen cells by lipid a: negative modulation of lipid a mitogenic activity by o-antigen polysaccharide.	we have investigated the regulatory effects of polysaccharide-rich subunits upon lipid a activity with the use of hybrid lps macromolecules of defined subunit composition. hybrid lps were constructed with polysaccharide-rich lps from escherichia coli o55:b5 and lipid a-rich lps from salmonella minnesota r595 by dissociation of the two parental lps species to monomeric solutions with deoxycholate, admixing these lps in various proportions and reassociation into high m.w. lps hybrid aggregates by ...	1985	2411800
cross-reactivity of rabbit antibodies to lipopolysaccharides of escherichia coli j5 and other gram-negative bacteria.	antiserum to rough gram-negative mutants such as escherichia coli j5 and salmonella minnesota re595 is thought to neutralize the toxic effects of lipopolysaccharides (lpss). to verify that such antisera are capable of binding heterologous endotoxins, we examined igg and igm class antibodies induced in rabbits to a variety of lpss. immunization with rough mutants or lipid a induced high igg antibody responses to the homologous purified lps and relatively low but significant responses to heterolog ...	1985	2413146
structural investigations on the inner core region of lipopolysaccharides from salmonella minnesota rough mutants.	the structure of the inner core region (l-glycero-d-mannoheptose/2-keto-3-deoxy-d-mannooctulosonic acid region) of lipopolysaccharides from salmonella minnesota rough mutants was investigated. using conventional methods (neutral sugar analysis, smith degradation and methylation analysis) combined with gas chromatography/mass spectrometry (gc/ms) of higher oligosaccharides (up to tetrasaccharide), the linkages of the core sugars of lipopolysaccharides from s. minnesota rough mutants, strains r4 ( ...	1985	2933084
the effect of bacterial lipopolysaccharide (lps) on histamine release from human basophils. i. enhancement of immunologic release by lps.	preincubation of human basophils with bacterial lipopolysaccharide (lps) purified from the heptose-deficient mutant salmonella minnesota r595 enhanced by an average of sixfold the response of peripheral blood basophils obtained from allergic donors to several allergens in vitro as judged by release of histamine. enhancement occurred at suboptimal, optimal, and supraoptimal concentrations of antigen. no effect was seen if basophils were from a nonallergic donor, and lps by itself rarely caused hi ...	1985	2578909
detection of a ganglioside antigen associated with small cell lung carcinomas using monoclonal antibodies directed against fucosyl-gm1.	monoclonal antibodies with an apparent specificity for fucosyl-gm1 (fuc-gm1) were produced by the immunization of mice with fuc-gm1 adsorbed to salmonella minnesota bacteria and fusion of the spleen cells with the myeloma cell line sp 2/0. the antibodies detected fuc-gm1 with a unique ceramide composition containing 2-hydroxy fatty acids in 11 of 12 cases of small cell carcinoma of the lung. trace amounts of fuc-gm1 were detected in 1 of 11 squamous epithelial cell lung carcinomas. fuc-gm1 was a ...	1986	3002616
lipopolysaccharide structures in enterobacteriaceae, pseudomonas aeruginosa, and vibrio cholerae are immunologically related to campylobacter spp.	to determine whether lipopolysaccharide (lps) structures of campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the lps of these strains with immune rabbit serum raised against six campylobacter jejuni strains and two campylobacter fetus strains. the lps studied were from salmonella minnesota wild type and ra to re mutants, salmonella typhi, escherichia coli, yersinia enterocolitica, vibrio cholerae, and pseudo ...	1986	3079730
a novel disialoganglioside (iv3neuaciii6neuaclc4) of human adenocarcinoma and the monoclonal antibody (fh9) defining this disialosyl structure.	this ganglioside is highly immunogenic, and immunization of mice with this disialoganglioside fraction coated on salmonella minnesota followed by fusion of immunized spleen cells with mouse myeloma and selection of the hybridoma by positive reactivity with the purified disialoganglioside resulted in the establishment of a hybridoma secreting immunoglobulin g2a antibody fh9 that reacts specifically with the ganglioside antigen above but not with monosialosyllactotetraosylceramide i (iv3neuaclc4), ...	1986	3087411
inhibition of x-ray or chemical carcinogen-induced neoplastic transformation of c3h10t1/2 fibroblasts by lipopolysaccharides.	oncogenic transformation of mouse 10t 1/2 fibroblasts induced upon exposure to x-ray or n-methyl-n'-nitro-n-nitrosoguanidine was suppressed if lipopolysaccharide (lps) was present in the culture medium. the suppressive effect of lps was exerted within 24 h after irradiation. suppression was dependent on the concentration of lps added and lps (2 micrograms/ml) derived from salmonella minnesota r595 reduced the number of transformed type iii foci per dish from 0.39 to 0.15. indomethacin (1 to 30 m ...	1986	3089580
characterization of a structural series of lipid a obtained from the lipopolysaccharides of neisseria gonorrhoeae. combined laser desorption and fast atom bombardment mass spectral analysis of high performance liquid chromatography-purified dimethyl derivatives.	monophosphoryl lipid a (mla) obtained from the lipopolysaccharides of serum-sensitive strains of neisseria gonorrhoeae was fractionated on a silicic acid column to yield the hexaacyl and pentaacyl mlas. the dimethyl derivative of the hexaacyl mla was analyzed by proton nuclear magnetic resonance spectroscopy. the dimethyl esters of hexaacyl and pentaacyl mlas were further purified by reverse-phase high performance liquid chromatography, and all of the peaks were analyzed by laser desorption mass ...	1986	3090037
serospecific antigens of legionella pneumophila.	serospecific antigens isolated by edta extraction from four serogroups of legionella pneumophila were analyzed for their chemical composition, molecular heterogeneity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunological properties. the antigens were shown to be lipopolysaccharides and to differ from the lipopolysaccharides of other gram-negative bacteria. the serospecific antigens contained rhamnose, mannose, glucosamine, and two unidentified sugars together with 2-ket ...	1986	3017918
structure-activity relationship of lipid a: comparison of biological activities of natural and synthetic lipid a's with different fatty acid compositions.	to investigate the structure-activity relationships, various biological activities, including pyrogenicity, lethal toxicity, elicitation of shwartzman reaction, mitogenicity and tumor necrosis factor (tnf)-inducing activity, were compared among natural and synthetic lipid a's differing in fatty acid composition. in all these tests, natural lipid a's from escherichia coli and salmonella minnesota and synthetic la-15-pp, which carries 3-hydroxy- and 3-acyloxy-tetradecanoyl groups at the 2, 3 and 2 ...	1986	2423510
the occurrence of anti-3-fucosyllactosamine antibodies and their cross-reactive idiotopes in preimmune and immune mouse sera.	the carbohydrate determinant 3-fucosyllactosamine (3-fl), gal(beta 1-4)[fuc alpha 1-3]glcnac-r, which is also known as ssea-1 or as the x determinant, is very immunogenic in balb/c mice. many monoclonal antibodies directed against this structure have been obtained by immunization of mice with murine teratocarcinomas and human carcinomas and myeloid cells. we have undertaken an analysis of the regulation of these antibodies and of their idiotypic, structural, and genetic diversity. we have descri ...	1986	2430018
the fatty acid content of the bordetella pertussis endotoxin.	the fatty acid content of bordetella pertussis endotoxin has been estimated by several methods. expressed as 3-hydroxytetradecanoic acid, it was 0.74 mumol (mg lyophilized material)-1, 0.38 mumol being ester-bound, and 0.32 mumol in amide linkage. reported molar ratios of ester-bound to amide-bound fatty acids in endotoxins of various bacterial species range from 2.4 to 2 in b. pertussis, to 5 to 2 in salmonella minnesota; according to these figures large differences must exist in the degree of ...	1986	2872266
contributions of c1q, bacterial lipopolysaccharide, and porins during attachment and ingestion phases of phagocytosis by murine macrophages.	in contrast to the s-form of salmonella minnesota, its re mutant binds to mouse peritoneal macrophages. the binding reaction triggers an oxidative burst, measured by a chemiluminescent reaction. the oxidative burst was abolished in the presence of either purified lipopolysaccharide or porins (outer membrane proteins) extracted from the re mutant, suggesting that both components are involved in binding of the re mutant to macrophages. in addition, fc-recognizing membrane structures on the macroph ...	1986	3005172
in vitro deacylation of lipopolysaccharide of salmonella minnesota by acanthamoeba castellanii enzymes.	enzymatic deacylation of the lipopolysaccharide isolated from a salmonella rd mutant by a cell-free preparation from acanthamoeba castellanii has been studied. the degradation was found to be dependent on the presence of a surface-active component (triton x-100) in the reaction mixture. the lipid a part of the lipopolysaccharide was the primary target of the enzymes, which cleaved with high efficiency the ester-bound long-chain nonhydroxylated and 3-hydroxylated acyl residues, i.e. lauric, myris ...	1986	3007130
pasteurellosis in laboratory rabbits: characterization of lipopolysaccharides of pasteurella multocida by polyacrylamide gel electrophoresis, immunoblot techniques, and enzyme-linked immunosorbent assay.	the lipopolysaccharides (lpss) of five isolates of pasteurella multocida from rabbits were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblots, and enzyme-linked immunosorbent assay. silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of purified unaggregated lpss resembled those of semirough strains of gram-negative enterobacteria and consisted of one or two bands that migrated within an interval just ahead or slightly behind the ...	1986	3744546
chemical, biological, and immunochemical properties of the chlamydia psittaci lipopolysaccharide.	the lipopolysaccharide (lps) of chlamydia psittaci was extracted from yolk sac-grown elementary bodies, purified, and characterized chemically, immunochemically, and biologically. the lps contained d-galactosamine, d-glucosamine, phosphorus, long-chain fatty acids, and 3-deoxy-d-manno-2-octulosonic acid in the molar ratio of approximately 1:2:2:6:5. the antigenic properties of the isolated lps were compared with those of the lps from chlamydia trachomatis and salmonella minnesota re by the passi ...	1986	3770953
neutrophil stimulation by lipid a and lipopolysaccharides from salmonella minnesota.		1986	3778779
relationship between immune system and gram-negative bacteria. acid-treated salmonella minnesota r595 (re) enhances immune responsiveness in patients with gynecologic malignancies.	nine patients with gynecologic malignancies (six cervix, two endometrium and one ovary) were injected with acid-treated salmonella minnesota r595 (re). patients received a total of four injections (at increasing doses from 0.5 to 3 micrograms) every 2 weeks. all patients, before treatment, had a severe impairment of their immune system either of cell-mediated immunity (leukocyte inhibiting factor activity) or non-specific immunity (polymorphonuclear cell and/or monocyte-mediated phagocytosis and ...	1986	3804533
effects of bacterial lipopolysaccharide on the binding of lymphocytes to endothelial cell monolayers.	preincubation of human umbilical vein endothelial cell (ec) monolayers with 1 ng to 10 micrograms/ml lipopolysaccharide (lps) increased the binding of t lymphocytes to ec. the effect was maximal at lps concentrations of 0.1 to 10 micrograms/ml, and occurred with lps derived from escherichia coli (serotypes 0111:b4 and 0127:b8), shigella flexneri (serotype 2a), serratia marcescens (serotype 0:3), and yersinia entercolitica (serotype 0:3). the increased binding appeared to be mediated primarily th ...	1986	3484495
biological activity of synthetic heptaacyl lipid a representing a component of salmonella minnesota r595 lipid a.	a synthetic lipid a (preparation 516), containing seven acyl groups and representing one component of natural free lipid a of salmonella minnesota r595, has been investigated for biological activity in a number of endotoxin test systems. it was found that the synthetic preparation was, in typical in vivo endotoxin tests (lethality, pyrogenicity, shwartzman reactivity) as well as in its antigenicity and macrophage activation capacity, significantly less active than natural salmonella lipid a. how ...	1986	3490368
adherence of lyme disease spirochetes to rat lymphocytes.	in the present work, the capacity of lyme disease (ld) spirochetes to spontaneously adhere to rat lymphocytes has been evaluated. borrelia organisms adhere to thymus, spleen, payer's patches and peripheral blood lymphocytes in a higher frequency than that observed with salmonella minnesota r345 (rb) used as reference strain. employing enriched splenic t and b cell populations, spirochete binding to b lymphocytes is more elevated than that obtained with salmonella, while similar percentage of t c ...	1986	3495084
characterization of murine monoclonal antibodies to escherichia coli j5.	twenty-eight independently derived monoclonal antibodies (mab) directed against escherichia coli j5 endotoxin were produced and characterized. each mab exhibited a specific titer by both radioimmunoassay and passive hemagglutination assay. most of the mab were of the immunoglobulin g isotype; however, several immunoglobulin m antibodies and one immunoglobulin a antibody were produced. when characterized for their capacity to cross-react with purified endotoxin preparations from several gram-nega ...	1986	3514463
elisa for detection of human antibodies to chlamydiae.	an enzyme-linked immunosorbent assay for the detection of human antibodies to chlamydiae is described which exploits the cross-react properties between the genus-specific antigen of chlamydiae and the relps constituent of the outer membrane of a salmonella minnesota mutant. of 100 random sera tested by elisa-relps and immunofluorescence 78% showed an absolute correlation, 15% were positive in immunofluorescence and negative in elisa and 7% were positive in elisa and negative in immunofluorescenc ...	1986	3537133
radioprotective properties of detoxified lipid a from salmonella minnesota r595.	in the past, the toxicity of bacterial lipopolysaccharide (lps) or its principal bioactive component, lipid a, has detracted from their potential use as radioprotectants. recently, a relatively nontoxic monophosphoryl lipid a (lam) that retains many of the immunobiologic properties of lps has been isolated from a polysaccharide deficient re mutant strain of salmonella minnesota (r595). the ability of the native endotoxic glycolipid (gl) from s. minnesota (r595) as well as diphosphoryl lipid a (l ...	1986	3488559
modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from bacteroides gingivalis.	two separate species of lipopolysaccharide (lps) from bacteroides gingivalis 381 have been isolated. sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated not only the heterogeneity of each species, but also that they represented high- and low-molecular-weight lps entities. although both contained the same carbohydrate and fatty acid components, the proportions of these differed between the lps species. the direct effects of these two species in modulation of bone resorption and ...	1986	3940999
inhibition by antibiotics of rb salmonella binding to human peripheral blood lymphocytes.	several lines of evidence point out that salmonella minnesota r345 (rb) possesses the capacity to adhere spontaneously to human peripheral blood lymphocytes. the binding is mediated via the lipopolysaccharide moiety of the bacterial outer membrane. in this report, we have evaluated the effects of various antibiotics on bacterial binding. our data show that trimethoprim/sulphamethoxazole, chloramphenicol and erythromycin significantly decrease rb binding, while gentamicin and sisomicin are withou ...	1986	3759441
immunotherapy of gram-negative bacterial sepsis. a single murine monoclonal antibody provides cross-genera protection.	previous experimental and clinical studies have demonstrated the ability of polyclonal antibody directed against the core lipopolysaccharide (lps)-lipid a component of endotoxin to reduce mortality. we sought to characterize the ability of a single murine monoclonal igg1 antibody (8a1 mab) to react to a variety of gram-negative microorganisms, to promote phagocytosis, and to provide protection during experimental murine sepsis. the 8a1 mab reacted to various gram-negative bacterial whole cell an ...	1986	3942500
endotoxin inactivating activity of rat serum.	the ability of rat serum to inactivate endotoxin (lps) was assessed with the aid of the limulus amebocyte lysate assay. following the addition of various amounts of endotoxin to normal serum the mixture was incubated for 1 hr at 37 degrees c and the residual endotoxin activity determined. one milliliter of rat serum inactivated between 5 and 10 micrograms escherichia coli lps per hour. heating serum for 45 min at 56 degrees c resulted in loss of 80-90% of the lps inhibitor (lpsi) activity. serum ...	1986	3945619
staining of o-specific polysaccharide chains of lipopolysaccharides with ruthenium red.	s-form lipopolysaccharides (lps) from klebsiella strain len-1 (o3: k1-) and from salmonella minnesota strain 1114 were positively stained with ruthenium red, whereas r-form lps from klebsiella strain len-111 (o3-: k1-) and ra, rb1, rcp+, rd1p-, and re lps from the respective mutant strains of s. minnesota were not or only faintly stained by such treatment. from these results it was concluded that ruthenium red stains the o-specific polysaccharide chains of lps. the appearance of stained preparat ...	1986	2431251
antibody-independent activation of the classical pathway of human serum complement by lipid a is restricted to re-chemotype lipopolysaccharide and purified lipid a.	incubation of most bacterial lipopolysaccharides (lps) with normal human sera at 37 degrees c activates the serum complement system, resulting in decreased levels of hemolytic complement. a panel of r-chemotype lps preparations isolated from salmonella minnesota rough mutant strains, as well as smooth wild-type lps from s. minnesota, escherichia coli o55-b5, serratia marcescens, and yersinia enterolitica, were used to examine the effect of lps polysaccharide chain length on lps lipid (lipid a)-d ...	1986	3744547
mechanism of human platelet activation by endotoxic glycolipid-bearing mutant re595 of salmonella minnesota.	the mechanism through which human blood platelets interact with gram-negative bacteria with well-defined structural variations in endotoxic lipopolysaccharide was studied. secretion of 14c-serotonin and aggregation of platelets separated from plasma proteins were observed on challenge with rough mutant re595 of salmonella minnesota possessing a glycolipid outer layer composed of lipid a and 2-keto-3-deoxyoctonate (kdo) but lacking heptose phosphate in the core and o-polysaccharide in its outer p ...	1986	3768528
synthetic salmonella-type lipid a antigen with high serological specificity.	a synthetic compound (compound 516), beta(1-6)-linked d-glucosamine disaccharide 1,4'-bisphosphate, which is acylated by (r)-3-hexadecanoyloxytetradecanoyl, (r)-3-hydroxytetradecanoyl, (r)-3-dodecanoyloxytetradecanoyl, and (r)-3-tetradecanoyloxytetradecanoyl groups at positions 2,3,2', and 3', respectively, exhibited in vitro antigenic reactivity of high specificity comparable to that of free lipid a from salmonella minnesota r595. this was confirmed by an enzyme-linked immunosorbent assay and a ...	1986	3941004
methylation analysis of the heptose/3-deoxy-d-manno-2-octulosonic acid region (inner core) of the lipopolysaccharide from salmonella minnesota rough mutants.	a modified methylation analysis is described which allows the elucidation of the structure of the inner core region [heptose/3-deoxy-d-manno-2-octulosonic acid (kdo)] of enterobacterial lipopolysaccharides (lps) of salmonella minnesota rough mutants (re, strain r595; and rd2p-, strain r4). methylation, carboxyl-reduction, remethylation, hydrolysis, carbonyl-reduction, and acetylation of the re-mutant lps yielded the 2,6-di-o-acetyl and 2,4,6-tri-o-acetyl derivatives of partially methylated 3-deo ...	1986	3756946
electrophoretic and serological characterization of the lipopolysaccharides of legionella pneumophila.	lipopolysaccharide (lps) is a major constituent of the outer membrane of gram-negative bacteria. we used sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteinase-k-digested cell lysates to provide preliminary data on the lps chemotypes for 20 strains of legionella pneumophila (serogroups 1 to 8). the profiles of all strains except chicago 2 (serogroup 6) were similar in the number, spacing, and size distribution of the bands visualized on silver-stained gels and were indicative of ...	1986	3519457
human monocyte production of interleukin-1: parameters of the induction of interleukin-1 secretion by lipopolysaccharides.	the role of lipopolysaccharide (lps) as an activator of human monocyte interleukin-1 (il-1) synthesis and secretion has been examined in this study. the results demonstrate that when blood monocytes are prepared under low endotoxin conditions, they do not spontaneously secrete il-1 activity. when cells are exposed to lps extracted from different bacterial species, there is variation seen in the potency, with lps from salmonella species being the most potent in inducing il-1 activity from human m ...	1986	3484775
induction of human interleukin 1 by bacterial and synthetic lipid a.	lipopolysaccharides (lps) are well known inducers of interleukin 1 (il 1). here we show that synthetic heptaacyl salmonella minnesota (compound 516) and synthetic e. coli type (compound 506) lipid a, as well as monodephospho part structures thereof, are able to induce il 1 production in human mononuclear cells. the 4'-monodephospho part structure (compound 505) was found to be the most active il 1 inducer as compared to compound 506 and the 1-monodephospho part structure (compound 504). syntheti ...	1986	3490388
immune responses to the lipopolysaccharides and capsular polysaccharides of haemophilus pleuropneumoniae in convalescent and immunized pigs.	the immunologic responses to a smooth-type lipopolysaccharide (lps) (hps-lps), a rough-type lps (hpr-lps), and a capsular-enriched polysaccharide preparation (hpc-ps) purified from haemophilus pleuropneumoniae were determined in pigs immunized with a commercial h. pleuropneumoniae cellular vaccine, in pigs experimentally infected with h. pleuropneumoniae, in control pigs, and in immunized rabbits. the ability of the preparations to induce lymphocyte blastogenesis and b-cell activation was determ ...	1986	3490442
monoclonal antibody against bacterial lipopolysaccharide cross-reacts with dna-histone.	monoclonal antibodies to bacterial lipopolysaccharide (lps) were prepared by fusing spleen cells from balb/c mice immunized with salmonella minnesota re 595 lps to the mouse myeloma cell line p3u1. one of them, designated rs01, revealed a strong positive antinuclear activity and reacted with dna-histone. rs01 also bound specifically to salmonella minnesota re 595 lps and eliminated the biological activity of lps. the salmonella completely inhibited the ana activity of rs01 and dna-histone blocke ...	1986	3542315
modulation of the biological activity of bacterial endotoxin by incorporation into liposomes.	in an attempt to define the mechanism by which endotoxin induces its biological activity, we studied the effect of the incorporation of lipopolysaccharide and lipid a into phospholipid vesicles (liposomes) on the stimulation of the macrophage cell-line raw 264.7 and on the coagulation of limulus amoebocyte lysate. the incorporation of salmonella minnesota smooth-and rough (re) lipopolysaccharide or primarily monophosphoryl lipid a into multilamellar and small unilamellar vesicles consisting of p ...	1987	3494081
the function of antibody and complement in the lysis of bacteria.	the factors controlling lysis of gram-negative bacteria by complement are being investigated systematically. the first question was how smooth salmonella minnesota, which has on its surface lipopolysaccharide with long o polysaccharide side chains, avoids lysis. rough organisms are serum sensitive. in both smooth and rough organisms, complement components are deposited on the surface and the lytic sequence proceeds to completion. however, with serum-resistant organisms the membrane attack comple ...	1987	3317749
investigations into the polymorphism of lipid a from lipopolysaccharides of escherichia coli and salmonella minnesota by fourier-transform infrared spectroscopy.	the polymorphism of lipid a, the endotoxic principle of the lipopolysaccharides of gram-negative bacteria, has been investigated in the fully hydrated state at temperatures between 5 degrees and 58 degrees c via fourier-transform infrared spectroscopy. these measurements were supplemented by x-ray diffraction, fluorescence intensity techniques and differential thermal analysis. up to three distinct phase transitions could be detected, with the main transition temperatures lying at approximately ...	1987	3549300
application of liposomes to generation of monoclonal antibody to glycosphingolipid: production of monoclonal antibody to ggose4cer.	liposomes were applied to the immunization with ggose4cer and screening for production of monoclonal antibody to ggose4cer. four-week-old and 22-week-old balb/c mice were immunized with ggose4cer and salmonella minnesota r595 lipopolysaccharides incorporated liposomes which were composed of dipalmitoyl-phosphatidylcholine and cholesterol. since antibody response to ggose4cer was higher in 22-week-old than 4-week-old balb/c mice after immunization, 22-week-old balb/c mice were used for the immuni ...	1987	3667566
experimental otitis media with effusion induced by intratympanic lipid a instillation.	otitis media with effusion was induced in guinea pigs by intratympanic instillation of lipid a, the lipid moiety of gram-negative bacterial lipopolysaccharide from salmonella minnesota re595. lipid a was chosen as an inducer because of its similar composition among various bacterial species. animals were killed from the first to 14th day after instillation of various concentrations (0.2, 2, 20, 200 micrograms/ml) of lipid a in 0.5% triethylamine. by 3 days after instillation, all experimental an ...	1987	3578674
a direct enzyme-linked immunosorbent assay (elisa) for antibodies to enterobacterial re core glycolipid and lipid a. results in healthy subjects and in patients infected by gram-negative bacteria.	we have developed an elisa for igm and igg antibodies to the core glycolipid (cgl) of the re mutant salmonella minnesota r 595, and to lipid a. anti-cgl antibodies have been detected in sera from 37% of healthy blood donors, whereas anti-lipid a activities were found in 13% of individuals only. the anti-cgl and anti-lipid a activities were examined in patients in a surgical intensive care unit, selected on the basis of a definite risk of infectious complications due to gram-negative bacteria. of ...	1987	3657701
approaches to augmenting the immunogenicity of the ganglioside gm2 in mice: purified gm2 is superior to whole cells.	the gangliosides gm2, gd2, and gd3 are differentiation antigens largely restricted to cells of neuroectodermal origin. they are expressed on most melanomas, astrocytomas, and neuroblastomas and have been shown to function as effective targets for monoclonal antibodies. in previous studies, we have immunized melanoma patients and mice with a series of melanoma cell vaccines containing these antigens, but have observed only occasional antibody responses. we report here the results of experiments i ...	1987	3805726
use of synthetic antigens to determine the epitope specificities of monoclonal antibodies against the 3-deoxy-d-manno-octulosonate region of bacterial lipopolysaccharide.	mouse monoclonal antibodies were raised against heat-killed bacteria of the re mutant r595 of salmonella minnesota and characterized by the passive hemolysis and passive hemolysis inhibition tests and by double immunodiffusion experiments using lipopolysaccharide (lps) from different rough mutants of s. minnesota and synthetic antigens. the latter were copolymerization products of acrylamide with the alpha- and beta-allylglycosides of 3-deoxy-d-manno-octulosonic acid (kdo) and the alpha-2,4-link ...	1987	2433221
antigenic and immunogenic properties of recombinants from salmonella typhimurium and salmonella minnesota rough mutants expressing in their lipopolysaccharide a genus-specific chlamydial epitope.	rough mutants from salmonella typhimurium and salmonella minnesota were transformed with a plasmid containing a 6.5-kilobase insert of dna from chlamydia trachomatis assumed to encode a glycosyltransferase. transformation resulted in the expression of a genus-specific chlamydial epitope on the lipopolysaccharide (lps) of the recombinant strains. proteinase k-digested whole-cell lysates of the recombinants and of controls were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...	1987	2433222
contribution of the mannan o side-chains to the adjuvant action of lipopolysaccharides.	klebsiella 03 lipopolysaccharide (lps) and escherichia coli 09 lps were previously shown to have potent adjuvant activities in augmenting antibody response and delayed-type hypersensitivity to protein antigens. the r form lps extracted from the o-specific polysaccharide-less mutants derived from klebsiella 03 and e. coli 09 strains were characterized by chemical and electrophoretic analyses. the adjuvant activities of the r-lps in augmenting antibody response and dth to protein antigens were muc ...	1987	2438205
artificial antigens. synthesis of polyacrylamide copolymers containing 3-deoxy-d-manno-2-octulopyranosylonic acid (kdo) residues.	starting from an anomeric mixture of methyl (allyl 4,5,7,8-tetra-o-acetyl-3-deoxy-alpha- and -beta-d-manno-2-octulopyranosid)onates, the glycosides sodium (allyl 3-deoxy-alpha- and -beta-d-manno-2-octulopyranosid)onate, sodium o-(sodium 3-deoxy-alpha-d-manno-2-octulopyranosylonate)-(2----4)-[allyl 3-deoxy-alpha-d-manno-2-octulopyranosid]onate and sodium (allyl 3-deoxy-7-o-beta-d-ribofuranosyl-beta-d-manno-2-octulopyranosid)++ +onate were prepared in several steps. radical copolymerization of the ...	1987	3319141
endotoxin neutralization with rabbit antisera to escherichia coli j5 and other gram-negative bacteria.	to study the mechanisms of protection against endotoxin challenge offered by antisera to smooth and rough gram-negative organisms, we have developed an assay to quantitate endotoxin neutralization based on inhibition of the limulus amoebocyte lysate test. dilutions of different bacterial lipopolysaccharides (lpss) were incubated with hyperimmune rabbit sera against escherichia coli o113, e. coli o18, and rough mutants e. coli j5 and salmonella minnesota re595 and were then combined with limulus ...	1987	3298063
requirement of a properly acylated beta(1-6)-d-glucosamine disaccharide bisphosphate structure for efficient manifestation of full endotoxic and associated bioactivities of lipid a.	several synthetic acylated glucosamine monophosphates, with structures corresponding to the nonreducing or reducing moiety of the lipid a of the escherichia coli or salmonella minnesota type, and a synthetic compound corresponding to a biosynthetic disaccharide lipid a precursor (designated ia or iva) were examined for their endotoxic and related bioactivities in comparison with those of the synthetic and bacterial parent molecules, i.e., acylated beta(1-6)-d-glucosamine disaccharide bisphosphat ...	1987	3539807
comparative studies of endotoxin uptake by isolated rat kupffer and peritoneal cells.	the process of uptake of endotoxin by cells of the reticuloendothelial system is of current interest. rabbit peritoneal macrophages have been used to study macrophage-endotoxin interactions and have suggested a receptor-mediated process. it is generally believed that the site of in vivo endotoxin clearance is the liver and that this clearance involves the kupffer cell population. in the current report, the uptake characteristics of iodine-125-labeled salmonella minnesota lipopolysaccharide (lps) ...	1987	2824379
effect of haemophilus influenzae type b lipopolysaccharide on complement activation and polymorphonuclear leukocyte function.	purified lipopolysaccharide (lps) from haemophilus influenzae type b (hib) was examined for its capacity to interact with human hemolytic complement, generate conversion products of c3, c4, and factor b, stimulate c5a activity, and affect human neutrophil chemiluminescence and phagocytosis. salmonella typhimurium lps and salmonella minnesota rb lps (r345 mutant) were examined for comparison. incubation of hib lps with human serum deficient in gamma-globulin or with normal human serum containing ...	1987	3324033
characterisation of protein co-extracted together with lps in escherichia coli, salmonella minnesota and yersinia enterocolitica.	the porin proteins of escherichia coli, yersinia enterocolitica, and salmonella minnesota were found to co-extract by the phenol-chloroform-petroleum ether method together with the r lipopolysaccharide of these strains. lipopolysaccharide free protein recovered from the phenolic residue of the phenol-chloroform-petroleum ether extract migrated as a mr 36-37,000 protein. we could demonstrate that the protein was extracted from bacteria as a high molecular weight protein-lipopolysaccharide complex ...	1987	3333795
vaccines containing purified gm2 ganglioside elicit gm2 antibodies in melanoma patients.	gm2, gd2, and gd3 gangliosides are expressed on the surface of human melanoma cells and represent potential targets for immunological control of melanoma growth by monoclonal antibodies and active immunization. the immunogenicity of gm2 was investigated by analyzing the humoral immune response of melanoma patients to vaccination with cell lines selected for high gm2 expression and with vaccines containing purified gm2. the whole-cell vaccine and vaccines containing purified gm2 and bacillus calm ...	1987	3472242
a new method for reduction of endotoxin contamination from protein solutions.	a method of reducing endotoxin contamination in protein-containing solutions is described here using a combination of polymyxin b-sepharose 4b (pb-seph 4b) affinity binding and endotoxin-protein dissociation with the dialyzable surfactant, octyl-beta-d-glucopyranoside (obdg). using the limulus amoebocyte lysate (lal) assay to detect endotoxin, greater than 1000-fold reduction of endotoxin reactivity could be accomplished from a contaminated commercial preparation of bovine catalase. importantly, ...	1987	3693909
responses of baboons to traditionally pyrogenic agents.	it is not clear whether baboons develop fever in response to endotoxin or other pyrogens. we injected various pyrogens intravenously in 12 unrestrained baboons (papio ursinus) and measured their body temperature using intra-abdominal radiotelemeters. serum iron concentration was also measured. the baboons developed fever after injection of killed staphylococcus aureus (5 x 10(7) organisms/kg). no significant fever was measured after injection of lipopolysaccharide (salmonella typhosa) (0.1, 8, 4 ...	1987	3621085
role of myeloperoxidase in the killing of naegleria fowleri by lymphokine-altered human neutrophils.	previously we have shown that human neutrophils treated with conditioned medium from phytohemagglutinin-stimulated mononuclear leukocytes (scm) in the presence of antisera have amoebicidal properties for naegleria fowleri, a pathogenic free-living amoeba. the data now presented show that neutrophils which lack myeloperoxidase (mpo) but have a normal oxygen-dependent respiratory burst could not be altered by scm to express the amoebicidal activity. catalase inhibited this amoebicidal activity of ...	1987	3032797
isolation and characterization of murine monoclonal antibodies specific for gram-negative bacterial lipopolysaccharide: association of cross-genus reactivity with lipid a specificity.	somatic cell hybrids secreting monoclonal antibodies against the core-glycolipid portion of enterobacterial endotoxin were derived from mice immunized with escherichia coli j5 or salmonella minnesota r595 heat-killed organisms or lipopolysaccharide (lps). eight antibodies were selected for their ability to cross-react with several members of a panel of gram-negative bacterial antigens in a radioimmunoassay. this panel represented five genera and two families of organisms: e. coli o111:b4, e. col ...	1987	3549565
enzyme immunoassay for the quantitation of immunoglobulin m class antibodies to salmonella minnesota r595 and escherichia coli j5 lipopolysaccharides.	the level of human immunoglobulin (igm) in plasma specific for the lipopolysaccharide of salmonella minnesota r595 (r595 lps) and escherichia coli j5 (j5 lps) was quantitated by an enzyme immunoassay (eia) in which purified antigen is adsorbed directly onto polystyrene-acrylic copolymer cuvettes. highly purified anti-j5 and r595 lps specific igm prepared by ion-exchange resin, gel filtration, and affinity resin chromatography were used as standards. the levels of specific igm were determined in ...	1987	3542358
acid-treated, naked bacteria as immune carriers for protein antigens.	salmonella minnesota r595 bacteria were stripped of their natural antigenic determinants to yield acid-treated, naked bacteria. the proteins, human apolipoprotein a1 and carcino-embryonic antigen, were adsorbed to naked bacteria and these complexes were used to immunise rabbits. although the antibody titres obtained were comparable to those achieved using freund's adjuvant emulsions, much less antigen was needed for immunisation. this technique could be of great value where the amount of protein ...	1987	2437205
demonstration of cross-reactive antibodies to smooth gram-negative bacteria in antiserum to escherichia coli j5.	we investigated the discrepancy between the broad cross-protection against gram-negative infections afforded by antiserum to escherichia coli j5 and its apparently narrow cross-reactivity in vitro. rabbits immunized with j5 bacteria produced antibodies to both the j5 lipopolysaccharide (lps; titer by elisa, 1:60,000) and lps from the re mutant of salmonella minnesota (i.e., to the ketodeoxyoctonate [kdo] and lipid a determinants; titer, 1:3,200). in highly diluted antiserum, titers of antibody t ...	1987	2439613
functional properties of salmonella minnesota rb-bound and rb-unbound cell fractions in elderly donors.	the capacity of salmonella minnesota r345 (rb) binding to human peripheral blood lymphocytes allows the recovery of rb-bound and rb-unbound cell populations that elicit different functions. here, we have applied this method to lymphocytes from aged individuals to evaluate the possibility that such an approach could reverse the senescence-related impaired immune responsiveness. in this regard we show that rb binding augments either spontaneous or t-dependent plaque-forming cell generation in rb-u ...	1987	3297381
antibody immunotherapy of gram-negative bacterial sepsis.	gram-negative bacterial sepsis continues to represent a significant cause of morbidity and mortality in hospitalized patients. currently available medical therapy (antimicrobial agents, hemodynamic monitoring, aggressive fluid resuscitation, and nutritional support) for this disease process has reduced but not eliminated the severe consequences that may ensue. recent investigations have demonstrated the ability of antibody directed against gram-negative bacterial lipopolysaccharide (lps or endot ...	1987	3299299
serological relationships between escherichia coli and salmonella smooth- and rough-mutant lipopolysaccharides as revealed by enzyme-linked immunosorbent assay for human immunoglobulin g antiendotoxin antibodies.	reactions of sera from healthy blood donors to smooth and rough mutant lipopolysaccharides (lps) from escherichia coli o111:b4 and salmonella minnesota which had been complexed with polymyxin b were determined by enzyme-linked immunosorbent assay. relative enzyme-linked immunosorbent assay reactivities were examined for prima facie evidence of cross-reactivity by analyses of correlation and relative scatter. patterns of reactivity were interpreted in relation to published structures. evidence wa ...	1987	3312008
effects of calmodulin inhibitors on rabbit synoviocyte phospholipase a2.	the effect of calmodulin inhibitors on synoviocyte phospholipase a2 activity was evaluated. cells were incubated with [3h]arachidonic acid after 24 hours to label phospholipids. [3h]prostaglandin e2 synthesis was stimulated by salmonella minnesota lipopolysaccharide (100 micrograms/ml). trifluoperazine, 35 microm, reduced lipopolysaccharide-stimulated [3h]prostaglandin e2 synthesis by 50%. in sonicated suspensions of cells, calcium-dependent phospholipase a2 activity was inhibited by trifluopera ...	1987	3118395
production of potent polyclonal antibodies to bacterial membrane amphiphiles.	lipid a (la), ketodeoxyoctonate (kdo) and lipoteichoic acids (lta) were used to produce homologous polyclonal antibodies. these haptens were administered to rabbits in differing immunogenic forms, using multiple intradermal and intraperitoneal injections with complete freund adjuvant. booster injections were either made intradermally with incomplete freund adjuvant or intravenously in saline. the immune-response was monitored regularly with an enzyme-immunoassay. lipid a and kdo covalently linke ...	1987	3123902
pilot study of intravenous administration of the acid-treated salmonella minnesota r595 (re) in cancer patients.	the clinical toxicity of acetic acid-treated "salmonella minnesota" r595 (re) organisms was evaluated in 24 cancer patients. bacteria were injected i.v. four times at increasing doses for a total of 6.5 micrograms. this therapeutic regimen was free of major side effects (one patient had fever higher than 38 degrees c and 10 patients complained of pruritus). furthermore, this bacterial preparation which possesses a more exposed lipid a on its surface, exhibited immunomodulating capacities in that ...	1987	2961115
prevention of lethal endotoxemia in actinomycin d-sensitized mice by incubation of salmonella minnesota r595 lipopolysaccharide with monoclonal antibodies to r595.	murine monoclonal antibodies reacting with lipopolysaccharide (lps) of salmonella minnesota strain r595 (re chemotype) were prepared, and tested for their ability to protect actinomycin d-sensitized mice against lethal endotoxemia. protection was found with some antibodies up to a 90-fold increase in ld50, whereas others exhibited no protection. the various protective antibodies did not all bind to the same epitope. the same applied for non-protective clones. protective and non-protective clones ...	1988	2467166
immunization with rough mutants of salmonella minnesota: protective activity of igm and igg antibody to the r595 (re chemotype) mutant.	we evaluated the immunoglobulin class responsible for the protective activity in serum obtained from humans and rabbits after immunization with the r595 (re chemotype) mutant of salmonella minnesota. whole serum obtained before immunization and the igg and igm fractions failed to protect mice against lethal challenge with viable klebsiella pneumoniae or morganella morganii or with salmonella typhi lipopolysaccharide (lps). the protective activity of postimmunization serum resided solely in igm a ...	1988	3042871
immunization with rough mutants of salmonella minnesota: initial studies in human subjects.	vaccines prepared from unheated and boiled, acetone-precipitated salmonella minnesota r595 (re chemotype mutant) were administered subcutaneously to 122 healthy volunteers. titers of antibody to re lipopolysaccharide, the basal core structure of endotoxin, as measured by indirect hemagglutination, rose in a dose-responsive fashion after immunization. febrile reactions, usually mild, occurred after 7% of injections with the highest doses (2.0 and 3.0 x 10(10) organisms), and mild local soreness a ...	1988	3042872
incorporation of lps in liposomes diminishes its ability to induce tumoricidal activity and tumor necrosis factor secretion in murine macrophages.	we investigated the effect of lipopolysaccharide (lps) incorporated into phospholipid vesicles (liposomes) on the induction of macrophage-mediated tumor cytotoxicity and tumor necrosis factor (tnf) secretion. the incorporation of salmonella minnesota rough (re)-lps into multilamellar or small unilamellar vesicles (liposomes) resulted in an 100- to 1,000-fold reduction in its potency to activate both the macrophage cell line raw 264.7 and murine thioglycolate elicited peritoneal macrophages to be ...	1988	3131472
effective inhibition of cardiolipin-binding antibodies in gram-negative infections by bacterial lipopolysaccharide.	anticardiolipin antibodies (aca) were detected by solid-phase enzyme immunoassay in the majority of sera from patients with gram-positive and gram-negative bacterial infections. the response involved all the major immunoglobulin classes igg, igm, and iga. the specificity of the aca was studied in competitive inhibition experiments with three putative antigens: cardiolipin, lipopolysaccharide (lps) isolated from salmonella minnesota, strain re 595, and synthetic escherichia coli lipid a. the bind ...	1988	3212387
glycolipid induced proliferation of lipopolysaccharide hyporesponsive c3h/hej splenocytes.	although the c3h/hej mouse is hyporesponsive to lipopolysaccharides (lps), certain forms of the lipid a fraction have been shown to stimulate cells from this mouse strain. to determine the role of the oligosaccharide chain length on the lipid a-induced proliferation of c3h/hej splenocytes, a panel of glycolipids from r-chemotypes (re, rc, and rd) and a nontoxic monophosphoryl lipid a (mpl) were tested. the mpl cells isolated from the mpl of salmonella minnesota, salmonella typhimurium, and the r ...	1988	3275732
immune carrier properties of acid-treated salmonella minnesota r595 bacteria. the immune response to tnp-bacterial conjugates in rabbits and mice.	salmonella minnesota r595 bacteria from which the core region of the lipopolysaccharide on the cell wall had previously been removed by mild acid treatment were trinitrophenylated. differing amounts of these trinitrophenyl naked bacterial conjugates (tnp-nb), covering a range of epitope densities, were used for immunising mice and rabbits via the intraperitoneal or intravenous routes without adjuvants. it was found that such acid-treated, naked bacteria were effective carriers for the covalently ...	1988	3280687
a procedure for the efficient incorporation of wild-type lipopolysaccharide into liposomes for use in immunological studies.	previous studies on the mechanism of action of lipopolysaccharides (lps) on macrophages have used wild-type lipopolysaccharide (wt-lps) containing liposomes. in these studies the endotoxin was incorporated into liposomes by suspending the wt-lps in the buffer used to rehydrate the lipid. using this approach (buffer method), we observed that less than 10% of salmonella minnesota smooth lps is incorporated into multilamellar vesicles (mlv). if the non-incorporated material is not effectively separ ...	1988	3263442
pulmonary oxygen toxicity in rats: prevention by pyrogenic diphosphoryl lipid a and potentiation by nontoxic monophosphoryl lipid a and lipid x.	pretreatment with gram-negative bacterial endotoxin protects rats from the lethal intrathoracic edema produced by continuous exposure to greater than 95% o2 for 3 days. to help elucidate structure-activity relationships of endotoxin, we administered lipid part-structures of endotoxin intraperitoneally (at dosages up to 5.0 mg/kg) to male sprague-dawley rats prior to exposure to greater than 99% o2 (at 760 mm hg) for 72 hr. we found that salmonella minnesota mutant re595 diphosphoryl lipid a prot ...	1988	3075066
structural heterogeneity regarding local shwartzman activity of lipid a.	the relation of chemical structure to local shwartzman activity of lipid a preparations purified by thin-layer chromatography from five bacterial strains was examined. two lipid a fractions from e. coli f515--ec-a2 and ec-a3--exhibited strong activity, similar to that of previous synthetic e. coli-type lipid a (compound 506 or la-15-pp). the ec-a3 fraction contained a component that appeared to be structurally identical to compound 506, and the main component of ec-a2 fraction was structurally s ...	1988	3057331
[preventive and therapeutic action of sera to the re mutant of salmonella minnesota in experimental generalized meningococcal infection].	the preparations of the blood plasma of humans and the sera of animals immunized with enterobacterial vaccine proved to have elevated titers of antibodies to deep determinants of the core of the gram-negative bacterial endotoxin molecule (to glycolipid of chemotype re) and protected animals from infection with live cultures or serogroup a, b, or c meningococcal endotoxins. sera from nonimmunized animals and normal donor blood plasma showed no protective activity. in experiments carried out on di ...	1988	3137743
evaluation of procoagulant activity production and other coagulative functions in cancer patients receiving acid treated salmonella minnesota r 595 (re).	blood clotting parameters were measured in six patients with gynecologic tumors following i.v. administration of acetic acid-treated salmonella minnesota r595 (re), which has been previously demonstrated to enhance impaired immune functions in the same patients. although these bacteria possess a more exposed lipid a at their surface which has the potential to stimulate the coagulative cascade, no evidence of plasma hypercoagulative state was detected. in fact, procoagulant activity generated by ...	1988	3181391
comparison of the opsonic activity of polyclonal and monoclonal antibodies raised against salmonella minnesota strain r595.	murine monoclonal antibodies and immune rabbit serum were raised against the rough mutant salmonella minnesota strain r595. these antibodies were tested for their opsonic activity against the homologous strain and the smooth wild type s. minnesota by luminol-dependent chemiluminescence and a microscopic assessment of phagocytosis. immune rabbit serum opsonised both strains. treatment with normal rabbit serum inhibited the phagocytic uptake of s. minnesota r595. none of the monoclonal antibodies ...	1988	2448462
characterization of the binding epitope of a monoclonal antibody to sulphatide.	an igg1 monoclonal antibody, sulph i, reacting with sulphatide (3'-sulphogalactosylceramide), was produced by immunizing balb/c mice with that glycolipid coated on salmonella minnesota bacterial membrane. radioimmunodetection of the binding of the monoclonal antibody to structurally related glycolipids adsorbed to microtitre plates or chromatographed on thin-layer plates was used to determine its binding epitope. the antibody showed similar binding avidity to three sulphated glycolipids: sulphat ...	1988	2455508
inhibition of activated nonresponder c3h/hej lymphocytes by lipopolysaccharide endotoxin.	the b lymphocytes and macrophages of lipopolysaccharide (lps) nonresponder c3h/hej mice were found to respond to certain r types of lps endotoxin in a fashion resembling that ordinarily seen with the cells from normal responder mice. dna synthesis, polyclonal antibody synthesis, and interleukin-1 activity were stimulated by bordetella pertussis lps and salmonella minnesota r595 lps, although to a lesser extent than with responder cells. mitogenesis stimulated by both lpss was inhibited by polymy ...	1988	2903122
are antimitochondrial antibodies in primary biliary cirrhosis induced by r(rough)-mutants of enterobacteriaceae?	antimitochondrial antibody (ama)-positive serum samples from 45 patients with primary biliary cirrhosis (pbc) and ama-negative serum samples from patients with chronic liver diseases, systemic lupus erythematosus, or rheumatoid arthritis were studied by an immunoblot technique with mitochondria from bovine heart and pig kidney and with several strains of gram-negative bacteria as antigens after separation by sodiumdodecylsulphate polyacrylamide gel electrophoresis. serum from patients with pbc r ...	1988	2903378
interaction of bordetella pertussis virulence components with neutrophils: effect on chemiluminescence induced by a chemotactic peptide and by intact bacteria.	the effect of secreted virulence components of bordetella pertussis on chemiluminescence (cl) of rabbit peritoneal neutrophils was determined with the chemotactic peptide n'-formyl-l-methionyl-l-leucyl-l-phenylalanine (fmlp) or intact b. pertussis as the stimulus. pertussis toxin (pt) inhibited the response to fmlp in a dose-dependent manner, although only after the neutrophils had been exposed to the toxin for greater than 15 min. both filamentous haemagglutinin (fha) and lipopolysaccharide (lp ...	1988	2908118
[specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants].	the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ...	1988	2464261
the significance of high-density lipoproteins (hdl) in the clearance of intravenously administered bacterial lipopolysaccharides (lps) in mice.	the direct immunofluorescence technique was used to study the presence of high-density lipoproteins (hdl) in the liver, spleen and kidney of mice before and after intravenous administration of purified lipopolysaccharides (lps) from escherichia coli o 75, salmonella abortus equi and salmonella minnesota r 595, as well as free lipid a. untreated mice had small granules of hdl in the hepatocellular cytoplasm, which appeared more pronounced after oral administration of fat. after intravenous admini ...	1988	3042575
comparison of the endotoxin-binding capacity of human transferrin and a human applicable immunoglobulin preparation.	data obtained by in vitro experiments concerning the binding kinetics of the transferrin-endotoxin interactions are provided. the separation of protein-bound and free tritiated endotoxin of e. coli o 111:b4 is achieved by a simple precipitation method using ethanol. the main features of this reaction are the velocity of the saturation--saturation is reached after 2-3 min--and the requirement of divalent cations. edta (ethylenediaminetetraacetic acid) strongly inhibits the binding reaction of end ...	1988	3052461