Publications

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transcriptomic changes reveal gene networks responding to the overexpression of a blueberry dwarf and delayed flowering 1 gene in transgenic blueberry plants.constitutive expression of the cbf/dreb1 for increasing freezing tolerance in woody plants is often associated with other phenotypic changes including dwarf plant and delayed flowering. these phenotypic changes have been observed when arabidopsis dwarf and delayed flowering 1 (ddf1) was overexpressed in a. thaliana plants. to date, the ddf1 orthologues have not been studied in woody plants. the aim of this study is to investigate transcriptomic responses to the overexpression of blueberry (vacci ...201728629320
nitrogen-source preference in blueberry (vaccinium sp.): enhanced shoot nitrogen assimilation in response to direct supply of nitrate.blueberry (vaccinium sp.) is thought to display a preference for the ammonium (nh4+) form over the nitrate (no3-) form of inorganic nitrogen (n). this n-source preference has been associated with a generally low capacity to assimilate the no3- form of n, especially within the shoot tissues. nitrate assimilation is mediated by nitrate reductase (nr), a rate limiting enzyme that converts no3- to nitrite (no2-). we investigated potential limitations of no3- assimilation in two blueberry species, ra ...201728578080
evaluation of attractants for monitoring drosophila suzukii (diptera: drosophilidae).drosophila suzukii matsumura is an economically important pest of soft and small fruit crops. unlike other drosophilid flies, d. suzukii is capable of infesting ripe and partially ripe fruit, which poses a significant pest management challenge, as there is no tolerance for infested or damaged fruit in the marketplace. as a result, producers in many regions rely on calendar-scheduled insecticide applications for d. suzukii management. in order to develop an integrated pest management approach, be ...201728444365
firmness at harvest impacts postharvest fruit softening and internal browning development in mechanically damaged and non-damaged highbush blueberries (vaccinium corymbosum l.).fresh blueberries are very susceptible to mechanical damage, which limits postharvest life and firmness. softening and susceptibility of cultivars "duke" and "brigitta" to developing internal browning (ib) after mechanical impact and subsequent storage was evaluated during a 2-year study (2011/2012, 2012/2013). on each season fruit were carefully hand-picked, segregated into soft (<1.60 n), medium (1.61-1.80 n), and firm (1.81-2.00 n) categories, and then either were dropped (32 cm) onto a hard ...201728443123
comparison of anti-inflammatory activities of an anthocyanin-rich fraction from portuguese blueberries (vaccinium corymbosum l.) and 5-aminosalicylic acid in a tnbs-induced colitis rat model.despite the actual therapeutic approaches for inflammatory bowel disease (ibd), efficient and secure alternative options remain a research focus. in this context, anthocyanins seem promising natural anti-inflammatory agents, but their action mechanisms and efficacy as compared with established drugs still require more clarification. the main aim of this study was to compare the anti-inflammatory action of a chemically characterized anthocyanin-rich fraction (arf), obtained from portuguese bluebe ...201728329021
phenolic acid profiling, antioxidant, and anti-inflammatory activities, and mirna regulation in the polyphenols of 16 blueberry samples from china.to investigate the anti-atherosclerosis related mechanism of blueberries, the phenolic acids (pas) content, antioxidant and anti-inflammatory activities, as well as the microrna (mirna) regulation of polyphenol fractions in blueberry samples from china were studied. sixteen batches of blueberries including 14 commercialized cultivars (reka, patriot, brigitta, bluecrop, berkeley, duke, darrow, northland, northblue, northcountry, bluesource, southgood, o'neal, and misty) were used in this study. s ...201728218703
the pentafluorophenyl stationary phase shows a unique separation efficiency for performing fast chromatography determination of highbush blueberry anthocyanins.a high-performance liquid chromatography method using an alternative pentafluorophenyl (pfp) core-shell stationary phase has been developed and used for rapid separation of 23 anthocyanins in a highbush blueberry bluehaven cultivar. a high efficiency of separation of anthocyanins was achieved in the core-shell column kinetex pfp, 150×4.6mm (particle size 2.6µm) with a 5×4.6mm precolumn, using a simple linear gradient elution with a mobile phase of acetonitrile and a water solution of 2% formic a ...201728213230
effects of daily blueberry consumption on circulating biomarkers of oxidative stress, inflammation, and antioxidant defense in postmenopausal women with pre- and stage 1-hypertension: a randomized controlled trial.oxidative stress and inflammation are central to the development of a number of chronic diseases including cardiovascular disease and previous research suggests that blueberry consumption may attenuate these processes. the present study investigated the effects of blueberries on blood biomarkers of oxidative stress, inflammation, and antioxidant defense in postmenopausal women with pre- and stage 1-hypertension. in a randomized, parallel-arm, double-blind, placebo-controlled clinical trial, 40 p ...201728059417
distinct physiological and metabolic reprogramming by highbush blueberry (vaccinium corymbosum) cultivars revealed during long-term uv-b radiation.despite the montreal protocol and the eventual recovery of the ozone layer over antarctica, there are still concerns about increased levels of ultraviolet-b (uv-b) radiation in the southern hemisphere. uv-b induces physiological, biochemical and morphological stress responses in plants, which are species-specific and different even for closely related cultivars. in woody plant species, understanding of long-term mechanisms to cope with uv-b-induced stress is limited. therefore, a greenhouse uv-b ...201727943328
the effects of long-term management on patterns of carbon storage in a northern highbush blueberry production system.perennial crops potentially provide a sink for atmospheric carbon. however, there is a poor understanding of how perennial crops differ in their carbon allocation patterns, and few studies have tested how agronomic practices such as fertilization influence long-term patterns of carbon allocation in actual production systems. in this study, we report results of a long-term field experiment that tested the individual and combined effects of organic matter incorporation and nitrogen fertilization o ...201727932219
a brief review of blue- and bilberries' potential to curb cardio-metabolic perturbations: focus on diabetes.some epidemiological studies suggest that increased consumption of anthocyanins is associated with lower risk of cardiovascular disease (cvd) and hypertension. also, high consumption of anthocyanin-rich fruit, such as berries, is associated with a lower risk of developing type 2 diabetes (t2d). in this review, we briefly yet critically assess the available evidence in support of an anti-diabetic role of anthocyanins derived from berries, especially bilberry (vaccinium myrtillus l., also known as ...201727748191
liquid chromatographic/electrospray ionization quadrupole/time of flight tandem mass spectrometric study of polyphenolic composition of different vaccinium berry species and their comparative evaluation.ultra-high-performance liquid chromatography coupled with high-resolution quadrupole-time of flight mass spectrometry with both negative and positive ionization was used for comprehensively investigating the phenolic and polyphenolic compounds in berries from three spontaneous or cultivated vaccinium species (i.e., vaccinium myrtillus, vaccinium uliginosum subsp. gaultherioides, and vaccinium corymbosum). more than 200 analytes, among phenolic and polyphenolic compounds belonging to the classes ...201727847974
characterization of polyphenol oxidase from blueberry (vaccinium corymbosum l.).polyphenol oxidase (ppo) was extracted and characterized from high-bush blueberries. ppo showed an optimum activity at ph 6.1-6.3 and 35°c, with the enzyme showing significant activity over a wide temperature range (25-60°c). catechol was the most readily oxidized substrate followed by 4-methylcatechol, dl-dopa, and dopamine. blueberry ppo showed a km of 15mm and vmax of 2.57 δa420nm/min×10-1, determined with catechol. ppo was completely inactivated in 20min at 85°c, however, after 30minat 75°c ...201727719900
profound hypoglycemia-ınduced by vaccinium corymbosum juice and laurocerasus fruit.an emergency intervention was performed in a 75-year-old male patient with hypoglycemic attack and blackout. although he was diagnosed with prediabetes before 2 years, he did not take any anti-diabetic drug or follow dietary advice. he drank vaccinium corymbosum l (vc) juice daily with a belief that it increases sexual potency. before the development of hypoglycemia, the patient had consumed about 500 ml vc juice in addition to eating 200-300 gram of laurocerasus officinalis (lo) fruit. the meas ...201725097289
managed bumble bees (bombus impatiens) (hymenoptera: apidae) caged with blueberry bushes at high density did not increase fruit set or fruit weight compared to open pollination.highbush blueberry (vaccinium corymbosum l.) is an important crop grown throughout florida. currently, most blueberry growers use honey bees (apis mellifera l.) to provide pollination services for highbush blueberries even though bumble bees (bombus spp.) have been shown to be more efficient at pollinating blueberries on a per bee basis. in general, contribution of bumble bees to the pollination of commercial highbush blueberries in florida is unknown. herein, we determined if managed bumble bee ...201728334127
diversity of fungal assemblages in roots of ericaceae in two mediterranean contrasting ecosystems.the plants belonging to the ericaceae family are morphologically diverse and widely distributed groups of plants. they are typically found in soil with naturally poor nutrient status. the objective of the current study was to identify cultivable mycobionts from roots of nine species of ericaceae (calluna vulgaris, erica arborea, erica australis, erica umbellate, erica scoparia, erica multiflora, arbutus unedo, vaccinium myrtillus, and vaccinium corymbosum). the sequencing approach was used to am ...201728302364
combined effect of ultrasound, heat, and pressure on escherichia coli o157:h7, polyphenol oxidase activity, and anthocyanins in blueberry (vaccinium corymbosum) juice.the objective of this study was to evaluate the effect of different treatments-heat treatment (ht), sonication (sc), thermosonication (ts), manosonication (ms), manothermal (mt), and manothermosonication (mts) on escherichia coli o157:h7, polyphenol oxidase (ppo), and anthocyanin content in blueberry juice. first, samples were treated at different temperatures (30, 40, 50, 60, 70, and 80°c) and power intensities (280, 420, 560, and 700w) for 10min. subsequently, samples were treated using combin ...201728427631
probiotic-mediated blueberry (vaccinium corymbosum l.) fruit fermentation to yield functionalized products for augmented antibacterial and antioxidant activity.the aim of this study was to investigate the fermentation of blueberry fruit with selected probiotic bacteria (bacillus amyloliquefaciens and lactobacillus brevis) and yeast (starmerella bombicola) isolated from fermented starfish for the extraction of functionalized products for biomedical applications. all probiotic-based fermented extracts showed augmented antibacterial and antioxidant activity compared to the control. biochemical parameters of viable cell count, titratable acidity, total phe ...201728690159
a plant growth-promoting symbiosis between mycena galopus and vaccinium corymbosum seedlings.typically, mycena species are viewed as saprotrophic fungi. however, numerous detections of mycena spp. in the roots of green plants suggest that a continuum from saprotrophy to biotrophy could exist. in particular, mycenoid species have repeatedly been found in ericaceae plant roots. our study asked whether (1) mycena species are commonly found in the roots of green ericaceae plants; (2) mycena sequences are limited to a single group/lineage within the genus; and (3) a mycena sp. can behave as ...201728842791
eradication and sensitization of methicillin resistant staphylococcus aureus to methicillin with bioactive extracts of berry pomace.the therapeutic roles of phenolic blueberry (vaccinium corymbosum) and blackberry (rubus fruticosus) pomace (commercial byproduct) extracts (bpe) and their mechanism of actions were evaluated against methicillin resistant staphylococcus aureus (mrsa). five major phenolic acids of bpe, e.g., protocatechuic, p. coumaric, vanillic, caffeic, and gallic acids, as well as crude bpe completely inhibited the growth of vegetative mrsa in vitro while bpe+methicillin significantly reduced mrsa biofilm form ...201728270804
cognitive improving effects by highbush blueberry (vaccinium crymbosum l.) vinegar on scopolamine-induced amnesia mice model.the present study aimed to evaluate the preventive effects of highbush blueberry (vaccinium corymbosum l.) vinegar (bv) on cognitive functions in a scopolamine (sco)-induced amnesia model in mice. in this study, sco (1 mg/kg, intraperitoneal injection) was used to induce amnesia. icr mice were orally administered donepezil (5 mg/kg), blueberry extract (120 mg/kg), and bv (120 mg/kg) for 7 days. after inducing cognitive impairment by sco, a behavioral assessment using behavior tests (i.e., y-maze ...201829260547
abscisic acid stimulates anthocyanin accumulation in 'jersey' highbush blueberry fruits during ripening.non-climacteric blueberry (vaccinium spp.) fruits accumulate high levels of anthocyanins during ripening, which are a good source of dietary antioxidants. this study examined the effects of exogenous abscisic acid (aba) application on fruit characteristics and anthocyanin accumulation in a northern highbush blueberry (v. corymbosum 'jersey') during development. fruits on shrubs were treated with 1gl-1 aba before the initiation of fruit colouration. application of aba temporarily increased the le ...201829120800
dual action of highbush blueberry proanthocyanidins on aggregatibacter actinomycetemcomitans and the host inflammatory response.the highbush blueberry (vaccinium corymbosum) has a beneficial effect on several aspects of human health. the present study investigated the effects of highbush blueberry proanthocyanidins (pacs) on the virulence properties of aggregatibacter actinomycetemcomitans and macrophage-associated inflammatory responses.201829321009
interactive effects of aluminum and cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (vaccinium corymbosum l.) plantlets cultivated in vitro.to evaluate the potential role of phenolic compounds in al and cd stress tolerance mechanisms, vaccinium corymbosum cv. legacy plantlets were exposed to different metal concentrations. the present study used an in vitro plant model to test the effects of the following treatments: 100μm al; 100μmal + 50μmcd; and 100μmal + 100μmcd during periods of 7, 14, 21 and 30 days. the oxidative damage was determined by the accumulation of malondialdehyde (mda) and hydrogen peroxide (h2o2). the antioxidant a ...201829294441
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