Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| modulation of vacuolar ph is required for replication of edwardsiella ictaluri in channel catfish macrophages. | previous in vitro work demonstrated that edwardsiella ictaluri produces an acid-activated urease that can modulate environmental ph through the production of ammonia from urea. additional work revealed that expression of the e. ictaluri type iii secretion system (t3ss) is upregulated by acidic ph. both the urease and the t3ss were previously shown to be essential to intracellular replication. in this work, fluorescence microscopy with lysotracker red dnd-99 (ltr) indicated that e. ictaluri-conta ... | 2014 | 24664505 |
| identification of differentially abundant proteins of edwardsiella ictaluri during iron restriction. | edwardsiella ictaluri is a gram-negative facultative anaerobe intracellular bacterium that causes enteric septicemia in channel catfish. iron is an essential inorganic nutrient of bacteria and is crucial for bacterial invasion. reduced availability of iron by the host may cause significant stress for bacterial pathogens and is considered a signal that leads to significant alteration in virulence gene expression. however, the precise effect of iron-restriction on e. ictaluri protein abundance is ... | 2015 | 26168192 |
| correction for santander et al., fur-regulated iron uptake system of edwardsiella ictaluri and its influence on pathogenesis and immunogenicity in the catfish host. | 2015 | 26556880 | |
| identification and mucosal expression analysis of cathepsin b in channel catfish (ictalurus punctatus) following bacterial challenge. | the mucosal surfaces of fish (skin, gill and intestine) constitute the primary line of defense against pathogen invasion. although the importance of fish mucosal surfaces as the first barriers against pathogens cannot be overstated, the knowledge of teleost mucosal immunity are still limited. cathepsin b, a lysosomal cysteine protease, is involved in multiple levels of physiological and biological processes, and playing crucial roles for host immune defense against pathogen infection. in this re ... | 2015 | 26497091 |
| the host effects of gambusia affinis with an antibiotic-disrupted microbiome. | while serving as critical tools against bacterial infections, antimicrobial therapies can also result in serious side effects, such as antibiotic-associated entercolitis. recent studies utilizing next generation sequencing to generate community 16s gene profiles have shown that antibiotics can strongly alter community composition and deplete diversity. however, how these community changes in the microbiota are related to the host side effects is still unclear. we have used the freshwater western ... | 2015 | 26475244 |
| the effects of feeding β-glucan to pangasianodon hypophthalmus on immune gene expression and resistance to edwardsiella ictaluri. | pangasianodon hypophthalmus (striped catfish) is an important aquaculture species and intensification of farming has increased disease problems, particularly edwardsiella ictaluri. the effects of feeding β-glucans on immune gene expression and resistance to e. ictaluri in p. hypophthalmus were explored. fish were fed 0.1% fungal-derived β-glucan or 0.1% commercial yeast-derived β-glucan or a basal control diet without glucan. after 14 days of feeding, the mrna expression of immune genes (transfe ... | 2015 | 26439415 |
| efficacy of florfenicol for control of mortality associated with edwardsiella ictaluri in three species of catfish. | the efficacy of florfenicol for control of mortality associated with edwardsiella icatluri was studied in fingerlings of channel catfish ictalurus puntatus (delta strain), blue catfish i. furcatus (d&b strain), and a hybrid catfish (delta strain channel catfish × d&b strain blue catfish). on day 0, fish were immersion challenged in 65-l aquaria. for each of the three species of catfish, 10 aquaria were randomly assigned to two treatment groups, either treated with florfenicol at 0 mg/kg of body ... | 2015 | 26306332 |
| complement regulatory protein genes in channel catfish and their involvement in disease defense response. | complement system is one of the most important defense systems of innate immunity, which plays a crucial role in disease defense responses in channel catfish. however, inappropriate and excessive complement activation could lead to potential damage to the host cells. therefore the complement system is controlled by a set of complement regulatory proteins to allow normal defensive functions, but prevent hazardous complement activation to host tissues. in this study, we identified nine complement ... | 2015 | 26111998 |
| oral vaccination of channel catfish against enteric septicemia of catfish using a live attenuated edwardsiella ictaluri isolate. | enteric septicemia of catfish (esc), caused by edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern united states. efforts to develop an effective esc vaccine have had limited industrial success. in commercial settings, esc vaccines are typically administered by immersion when fry are transferred from the hatchery to rearing ponds. while this approach is a practical method of mass delivery, this strategy administers vaccines to very y ... | 2015 | 26030354 |
| the effects of feeding immunostimulant β-glucan on the immune response of pangasianodon hypophthalmus. | immunostimulants are food additives used by the aquaculture industry to enhance the immune response of fish, and although β-glucans are now commonly used for this purpose in aquaculture, little is known about their effects on the immune response of pangasianodon hypophthalmus. thus, a variety of immune parameters (e.g. phagocytosis, respiratory burst, lysozyme, complement, peroxidase, total protein, total anti-protease, total igm, natural antibody titres, and specific igm titres) was examined in ... | 2015 | 25921238 |
| molecular cloning and functional characterization of cyclophilin a in yellow catfish (pelteobagrus fulvidraco). | cyclophilin a (cypa) is a ubiquitously expressed protein which involves in diverse pathological conditions including infection and inflammation. in this report, a cypa gene (designated as yc-cypa) was cloned from yellow catfish (pelteobagrus fulvidraco) which is an important cultured fish species in asian countries. the open reading frame (orf) of yc-cypa encoded a polypeptide of 164 amino acids with calculated molecular weight of 17.70 kda. the deduced amino acid sequences of the yc-cypa shared ... | 2015 | 25882636 |
| hsp90, hsp60 and shsp families of heat shock protein genes in channel catfish and their expression after bacterial infections. | heat shock proteins (hsps) are a suite of highly conserved proteins whose expressions are generally induced by elevated temperature. however, many hsps play important roles in both innate and adaptive immunity. on the basis of our previous work on hsp40 and hsp70 gene families in channel catfish (ictalurus punctatus), the objective of this study was to characterize hsp90, hsp60, hsp10, and small hsp genes, and to investigate their expression profiles after bacterial infections. a total of 20 hsp ... | 2015 | 25827625 |
| identification and expression analysis of 26 oncogenes of the receptor tyrosine kinase family in channel catfish after bacterial infection and hypoxic stress. | receptor tyrosine kinases (rtks) are high-affinity cell surface receptors for many polypeptide growth factors, cytokines and hormones. rtks are not only key regulators of normal cellular processes, but are also involved in the progression of many types of tumors, and responses to various biotic and abiotic stresses. catfish is a primary aquaculture species in the united states, while its industry is drastically hindered by several major diseases including enteric septicemia of catfish (esc) that ... | 2015 | 25722053 |
| effects of a phytogenic feed additive on growth performance, susceptibility of channel catfish to edwardsiella ictaluri and levels of mannose binding lectin. | a study was conducted to investigate the effect of a phytogenic feed additive (digestarom® p.e.p. mge; containing the essential oils carvacrol, thymol, anethol, and limonene) on growth performance and disease susceptibility to edwardsiella ictaluri. two hundred and fifty juvenile channel catfish, ictalurus punctatus (7.2 ± 0.1 g) were allotted into the following treatments: control (floating diet) and eo (floating diet supplemented with essential oils). the fish were fed their respective diets f ... | 2015 | 25659231 |
| identification, phylogeny and expression analysis of suppressors of cytokine signaling in channel catfish. | the suppressors of cytokine signaling (socs) family genes play important roles in regulating a variety of signal transduction pathways that are involved in immunity, growth and development. because of their importance, they have been extensively studied in mammalian species, but they have not been systematically studied among teleost fish species. in this study, a total of 12 socs genes were characterized to understand the molecular mechanisms of socs function in channel catfish. phylogenetic an ... | 2015 | 25555134 |
| expression of tumor suppressor genes in channel catfish after bacterial infections. | tumor suppressor genes are negative regulators of tumor formation. while their anti-tumor functions have been well studied, they have been found to be also involved in immune responses and innate immunity. in this study, 21 tumor suppressor genes in channel catfish (ictalurus punctatus) were characterized. phylogenetic and syntenic analyses allowed annotation of all 21 catfish tumor suppressor genes. the expression profiles of the 21 catfish tumor suppressor genes were determined using the rna-s ... | 2015 | 25453578 |
| antibacterial activities of metabolites from platanus occidentalis (american sycamore) against fish pathogenic bacteria. | one approach to the management of common fish diseases in aquaculture is the use of antibiotic-laden feed. however, there are public concerns about the use of antibiotics in agriculture and the potential development of antibiotic resistant bacteria. therefore, the discovery of other environmentally safe natural compounds as alternatives to antibiotics would benefit the aquaculture industries. four natural compounds, commonly called platanosides, [kaempferol 3-o-α-l-(2″,3″-di-e-p-coumaroyl)rhamno ... | 2015 | 27790379 |
| genome modifications and cloning using a conjugally transferable recombineering system. | the genetic modification of primary bacterial disease isolates is challenging due to the lack of highly efficient genetic tools. herein we describe the development of a modified pcr-based, λ red-mediated recombineering system for efficient deletion of genes in gram-negative bacteria. a series of conjugally transferrable plasmids were constructed by cloning an orit sequence and different antibiotic resistance genes into recombinogenic plasmid pkd46. using this system we deleted ten different gene ... | 2015 | 28352570 |
| titanium dioxide nanoparticles enhance mortality of fish exposed to bacterial pathogens. | nano-tio2 is immunotoxic to fish and reduces the bactericidal function of fish neutrophils. here, fathead minnows (pimephales promelas) were exposed to low and high environmentally relevant concentration of nano-tio2 (2 ng g(-1) and 10 μg g(-1) body weight, respectively), and were challenged with common fish bacterial pathogens, aeromonas hydrophila or edwardsiella ictaluri. pre-exposure to nano-tio2 significantly increased fish mortality during bacterial challenge. nano-tio2 concentrated in the ... | 2015 | 25884347 |
| draft genome sequences of edwardsiella ictaluri strains ladl11-100 and ladl11-194 isolated from zebrafish danio rerio. | here, we report the draft genome sequences of edwardsiella ictaluri strains ladl11-100 and ladl11-194, two isolates from natural outbreaks of edwardsiellosis in the zebrafish danio rerio, as well as the sequences of the plasmids carried by the zebrafish strain of e. ictaluri. | 2015 | 26679579 |
| comparison of edwardsiella ictaluri isolates from different hosts and geographic origins. | the intraspecific variability of e. ictaluri isolates from different origins was investigated. isolates were recovered from farm-raised catfish (ictalurus punctatus) in mississippi, usa, tilapia (oreochromis niloticus) cultured in the western hemisphere and zebrafish (danio rerio) propagated in florida, usa. these isolates were phenotypically homologous and antimicrobial profiles were largely similar. genetically, isolates possessed differences that could be exploited by repetitive-sequence-medi ... | 2016 | 26661707 |
| expression profile analysis of two cathepsin s in channel catfish (ictalurus punctatus) mucosal tissues following bacterial challenge. | cathepsin s belongs to the papain family of cysteine protease, and is considered to play key roles in immune responses after bacterial challenge. however, despite the recognized importance of cathepsin s in immunity, no studies have systematically characterized cathepsin s in catfish. in this regard, here, we characterized the cathepsin s gene family in channel catfish, and investigated their expression patterns following two different gram-negative bacterial challenge. in the present study, two ... | 2016 | 26626584 |
| ferric hydroxamate uptake system contributes to edwardsiella ictaluri virulence. | edwardsiella ictaluri is a gram-negative facultative intracellular pathogen causing enteric septicemia in fish, particularly in channel catfish. ferric iron is an essential micronutrient for bacterial survival, and some bacterial pathogens use secreted hydroxamate-type siderophores to chelate iron in host tissues. siderophore-iron complexes are taken up by these bacteria via the ferric hydroxamate uptake (fhu) system. in e. ictaluri, the fhu system consists of fhuc, fhud, fhub, and fhua genes. h ... | 2016 | 27666508 |
| involvement of tolq and tolr genes in edwardsiella ictaluri virulence. | edwardsiella ictaluri is a gram-negative intracellular facultative pathogen causing enteric septicemia of channel catfish (esc). the tol system, consisting of four envelope proteins tolq, tolr, tola, and tolb, are required for colicin import and contributes to bacterial virulence in several pathogenic bacteria. however, the tol system and its importance in e. ictaluri virulence have not been investigated. here we present construction and evaluation of the e. ictaluri tolq, tolr and tolqr mutants ... | 2016 | 27622343 |
| edwardsiella ictaluri infection in pangasius catfish imported from west bengal into the southern caribbean. | in response to a mortality event, seven pangasius catfish (pangasianodon hypophthalmus) were submitted to the university of the west indies, school of veterinary medicine, trinidad and tobago, for diagnostic evaluation. these fish were part of a consignment that arrived from kolkata two weeks earlier. fish presented with perianal haemorrhage and blister-like swellings on the skin which ruptured to leave ulcers. edwardsiella ictaluri was consistently recovered from the brain and skin. repetitive ... | 2016 | 27592704 |
| ompn, outer membrane proteins of edwardsiella ictaluri are potential vaccine candidates for channel catfish (ictalurus punctatus). | outer membrane proteins (omps) are a class of proteins that reside in the outer membrane of gram-negative bacteria. omps act as epitopes and are potential vaccine candidates. outer membrane protein n (ompn) is a component of the outer membrane of edwardsiella ictaluri (e. ictaluri). in a previous study, the ompn1-, ompn2-, ompn3-encoding genes of e. ictaluri were cloned, and here they were expressed in escherichia coli. western blotting showed that these three proteins had molecular weights of ∼ ... | 2016 | 27567702 |
| molecular and functional characterization of liver x receptor in ayu, plecoglossus altivelis: regulator of inflammation and efferocytosis. | liver x receptors (lxr) are modulators of metabolic processes and inflammation in mammals as nuclear receptors. however, the precise function of lxr in teleosts remains unclear. here, we characterized a lxr gene (palxr) from ayu, plecoglossus altivelis. the palxr transcript was expressed widely in all tissues studied, and changes in expression were observed in tissues and monocytes/macrophages (mo/mφ) upon infection with the bacterium edwardsiella ictaluri. palxr activation decreased the mrna ex ... | 2016 | 27539204 |
| expression of bcl-2 genes in channel catfish after bacterial infection and hypoxia stress. | bcl-2 proteins are of vital importance in regulation of apoptosis, and are involved in a number of biological processes such as carcinogenesis and immune responses. bcl-2 genes have been well studied in mammals, while they are not well investigated in teleost fish including channel catfish, the major aquaculture species in the united states. in this study, we identified 34 bcl-2 genes from the channel catfish genome, and verified their identities by conducting phylogenetic and syntenic analyses. ... | 2016 | 27353474 |
| chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (pangasianodon hypophthalmus, s.) and leads to excessive inflammatory response during bacterial infection. | hyperosmotic stress has often been investigated from osmoregulation perspectives while the effects of such stress on the immune capacity remain largely unexplored. in this study, striped catfish were submitted to three salinity profiles (freshwater, low saline water, saline water) during 20 days, followed by infection with a virulent bacteria, edwardsiella ictaluri, responsible for the enteric septicaemia of catfish. osmoregulatory (plasma osmolality, gill na(+)k(+)atpase), immune (blood cells, ... | 2016 | 27346159 |
| effect of oligochitosan and oligo-β-glucan supplementation on growth, innate immunity, and disease resistance of striped catfish (pangasianodon hypophthalmus). | oligochitosan (cos) and oligo-β-glucan (βog) were prepared by gamma co-60 irradiation of chitosan/h2 o2 and β-glucan/h2 o2 solutions. the striped catfish (pangasianodon hypophthalmus) was fed diets containing 0-200 mg cos, βog, and a mixture of cos/βog per kg feed for 45 days, and then challenged with edwardsiella ictaluri bacterium. the effects of supplemented cos, βog, and a mixture of cos/βog on immune stimulation and growth performance in striped catfish were investigated. the results indica ... | 2016 | 27245476 |
| synergic stress in striped catfish (pangasianodon hypophthalmus, s.) exposed to chronic salinity and bacterial infection: effects on kidney protein expression profile. | hyperosmotic stress has often been investigated from osmoregulation perspectives while the effects of such stress on the immune capacity remain largely unexplored. in this study, striped catfish were submitted to a gradual hyperosmotic stress (up to 20ppt), followed by an infection with a virulent strain of an intracellular bacteria, edwardsiella ictaluri. physiological parameters (plasma osmolarity, gill nak atpase) have been investigated. moreover, a label free quantitative proteomics workflow ... | 2016 | 27155471 |
| influence of lipopolysaccharide outer-core in the intrinsic resistance to antimicrobial peptides and virulence in edwardsiella ictaluri. | the genus edwardsiella consists of bacteria with an intrinsic resistance to cyclic cationic antimicrobial peptides (camps). edwardsiella ictaluri, a pathogen of the catfish (ictalurus punctatus) and the causative agent of a systemic infection, is highly resistant to camps. previously, we determined that the oligo-polysaccharide (o-ps) of the lipopolysaccharide (lps) does not play a role in the e. ictaluri camp resistance and an intact core-lipid a structure is necessary for camps resistance. her ... | 2016 | 26945561 |
| phosphoinositide 3-kinase family in channel catfish and their regulated expression after bacterial infection. | the phosphoinositide-3-kinase (pi3ks) family of lipid kinases is widely conserved from yeast to mammals. in this work, we identified a total of 14 members of the pi3ks from the channel catfish genome and transcriptome and conducted phylogenetic and syntenic analyses of these genes. the expression profiles after infection with edwardsiella ictaluri and flavobacterium columnare were examined to determine the involvement of pi3ks in immune responses after bacterial infection in catfish. the results ... | 2016 | 26772478 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry based identification of edwardsiella ictaluri isolated from vietnamese striped catfish (pangasius hypothalamus). | edwardsiella (e.) ictaluri is a major bacterial pathogen that affects commercially farmed striped catfish (pangasius hypothalamus) in vietnam. in a previous study, 19 strains of e. ictaluri collected from striped catfish were biochemically identified with an api-20e system. here, the same 19 strains were used to assess the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms; applied using a maldi biotyper) to conduct rapid, easy and accurate iden ... | 2016 | 26726022 |
| effect of poly-β-hydroxybutyrate on growth and disease resistance of nile tilapia oreochromis niloticus juveniles. | the growth promoting effect of the bacterial storage compound poly-β-hydroxybutyrate (phb) has been studied for young fish of high trophic level (european sea bass) and intermediate trophic level (siberian sturgeon). here, the effect of phb on growth, digestive enzyme activities, body composition and diseases resistance of juvenile nile tilapia (oreochromis niloticus) of low trophic level was investigated. although dietary phb supplementation (5, 25 and 50 g phb kg(-1) formulated semi-purified d ... | 2016 | 26711027 |
| septin genes in channel catfish (ictalurus punctatus) and their involvement in disease defense responses. | septins are an evolutionarily conserved family of gtp-binding proteins. they are involved in diverse processes including cytokinesis, apoptosis, infection, neurodegeneration and neoplasia. in this study, through thorough data mining of existed channel catfish genomic resources, we identified a complete set of 15 septin genes. septins were classified into four subgroups according to phylogenetic analysis. extensive comparative genomic analysis, including domain and syntenic analysis, supported th ... | 2016 | 26700173 |
| genome-wide identification of hsp70 genes in channel catfish and their regulated expression after bacterial infection. | heat shock proteins 70/110 (hsp70/110) are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions. besides the chaperone and housekeeping functions, they are also known to be involved in immune response during infection. in this study, we identified 16 hsp70/110 geness in channel catfish (ictalurus punctatus) through in silico analysis using rna-seq and genome databases. among them 12 members of hsp70 (hspa) fa ... | 2016 | 26693666 |
| identification and characterization of putative translocated effector proteins of the edwardsiella ictaluri type iii secretion system. | edwardsiella ictaluri, a major pathogen in channel catfish aquaculture, encodes a type iii secretion system (t3ss) that is essential for intracellular replication and virulence. previous work identified three putative t3ss effectors in e. ictaluri, and in silico analysis of the e. ictaluri genome identified six additional putative effectors, all located on the chromosome outside the t3ss pathogenicity island. to establish active translocation by the t3ss, we constructed translational fusions of ... | 2016 | 27303737 |
| antimicrobial activity of chitosan and a chitosan oligomer against bacterial pathogens of warmwater fish. | the antibacterial activities of chitosan (cs) and its derivative chitosan oligosaccharide lactate (col) were evaluated against aeromonas hydrophila, edwardsiella ictaluri and flavobacterium columnare, three highly pathogenic bacteria of warmwater finfish. | 2017 | 28371006 |
| effects of the dietary supplementation of mixed probiotic spores of bacillus amyloliquefaciens 54a, and bacillus pumilus 47b on growth, innate immunity and stress responses of striped catfish (pangasianodon hypophthalmus). | the study used the mixed probiotics of bacillus amyloliquefaciens 54a and b. pumilus 47b isolated from striped catfish (pangasianodon hypophthalmus) intestine aiming to stimulate growth performance, innate immunity, stress tolerance of striped catfish. the average weight gain (awg), specific growth rate (sgr), and feed conversion ratio (fcr) were analyzed after fish were fed the mixture of probiotics (b. amyloliquefaciens 54a and b. pumilus 47b) at concentrations of 1 × 10(8), 3 × 10(8), and 5 × ... | 2017 | 27836719 |
| validation of fermentation and processing procedures for the commercial-scale production of a live, attenuated edwardsiella ictaluri vaccine for use in channel catfish aquaculture. | mortality associated with edwardsiella ictaluri infection is a serious impediment to the commercial production of fingerling channel catfish ictalurus punctatus. a patented, live, attenuated, orally delivered vaccine has been developed that offers exceptional protection against e. ictaluri infection in both laboratory and small-scale pond trials. further vaccine development is contingent on the successful completion of large-scale field trials that accurately reflect industry conditions. this cu ... | 2017 | 28376313 |
| chronic hyperosmotic stress inhibits renal toll-like receptors expression in striped catfish (pangasianodon hypophthalmus, sauvage) exposed or not to bacterial infection. | toll-like receptors (tlrs) are the first innate receptors in recognizing pathogen-associated molecular patterns. in fish, upregulation of toll-like receptors during infection has been largely demonstrated while the effects of abiotic stressors on their expression remain poorly investigated. in this study, striped catfish were submitted during 20 days to three salinity profiles (freshwater, low saline water, saline water), followed by injection of a bacterial strain of edwardsiella ictaluri. the ... | 2017 | 28344170 |
| the nck and abi adaptor genes in catfish and their involvement in esc disease response. | adaptor proteins non-catalytic region of tyrosine kinase (nck) and abelson interactor (abi) are crucial for disease response. nck1 was identified to be a candidate gene for enteric septicemia of catfish (esc) disease resistance, and was speculated to play similar roles during esc and enteropathogenic escherichia coli (epec) pathogenicity. abi1 was reported as a positional candidate gene for bacterial cold water disease (bcwd) resistance in rainbow trout. in this study, three nck genes and six ab ... | 2017 | 28341353 |
| identification and characterization of a β-defensin gene involved in the immune defense response of channel catfish, ictalurus punctatus. | antimicrobial peptides are small peptides that play important roles in a host's innate immune response. as an important antimicrobial peptide, β-defensin widely distribute in mammals, insects and plants with broad-spectrum antimicrobial activity. in this study, the β-defensin gene of the channel catfish, ictalurus punctatus, was cloned, sequenced, and subjected to a bioinformatic analysis. the β-defensin gene of the channel catfish contains three exons and two introns, and encodes a precursor pe ... | 2017 | 28340427 |
| the chemokinome superfamily: ii. the 64 cc chemokines in channel catfish and their involvement in disease and hypoxia responses. | chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. based on the arrangement of the first four cysteine residues, they are classified into cc, cxc, c and cx3c subfamilies. in this study, a complete set of 64 cc chemokine ligand (ccl) genes was systematically identified, annotated, and characterized from the channel catfish genome. extensive phylogenetic and comparative genomic analyses supported their ... | 2017 | 28322933 |
| rainbow trout (oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens. | commensal bacteria co-exist on the mucosal surfaces of all vertebrates. the host's mucosal immune system must tolerate commensals while fighting pathogens. one of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (igs) across epithelial barriers, which is achieved via the polymeric immunoglobulin receptor (pigr). rainbow trout pigr is known to transport igt and igm across epithelia. however, other biological functions for trout pigr or t ... | 2017 | 28150752 |
| gwas analysis of qtl for enteric septicemia of catfish and their involved genes suggest evolutionary conservation of a molecular mechanism of disease resistance. | disease problems cause major economic losses for the aquaculture industries. in catfish, enteric septicemia of catfish (esc), caused by the bacterial pathogen edwardsiella ictaluri, is the leading disease problem, causing tens of millions of dollars of annual economic losses. in this study, we conducted a genome-wide association study to determine quantitative trait loci (qtl) for resistance against esc using an interspecific hybrid system. five hundred fish were used in the analysis and 192 phe ... | 2017 | 27826737 |
| identification, annotation and expression analysis of 29 rho gtpase genes from channel catfish (ictalurus punctatus) after bacterial infections. | the rho family gtpases are a group of small monomeric g proteins, which are molecular switches in signaling pathways. they have been known to regulate a diverse range of cellular processes including actin cytoskeleton rearrangement and microtubule dynamics. in particular, their participations in immune responses are also significant. however, little information of the rho gtpases is available in teleost including channel catfish, an economically important species and one of the best teleost mode ... | 2017 | 27765605 |
| analysis of apolipoprotein genes and their involvement in disease response of channel catfish after bacterial infection. | apolipoproteins are protein component of plasma lipoproteins. they exert crucial roles in lipoprotein metabolism and serve as enzyme cofactors, receptor ligands, and lipid transfer carriers in mammals. in teleosts, apolipoproteins are also involved in diverse processes including embryonic and ontogenic development, liver and digestive system organogenesis, and innate immunity. in this study, we identified a set of 19 apolipoprotein genes in channel catfish (ictalurus punctatus). phylogenetic ana ... | 2017 | 27640368 |
| in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens. | antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ... | 2017 | 28732514 |
| lesions caused by virulent aeromonas hydrophila in farmed catfish ( ictalurus punctatus and i. punctatus × i. furcatus) in mississippi. | in 2009, a virulent strain of aeromonas hydrophila caused severe disease and high mortalities (motile aeromonad septicemia variant [masv]) in farmed channel catfish ( ictalurus punctatus) and hybrid catfish ( i. punctatus × i. furcatus) in eastern mississippi and alabama. as is common in mas, there is severe hemorrhagic dermatitis with ulceration, as well as abdominal hyperemia, petechiation, and mild ascites. additional findings in masv cases include panophthalmitis and orbital cellulitis, lead ... | 2017 | 28482758 |
| biofilm formation on aquaculture substrates by selected bacterial fish pathogens. | the objective of this study was to determine whether common bacterial catfish pathogens could attach and colonize surfaces commonly found in aquaculture facilities. in addition, we evaluated the role of calcium in biofilm formation. attachment to polystyrene plates was used to quantify biofilm formation by five bacterial pathogens (i.e., flavobacterium columnare, aeromonas hydrophila, edwardsiella ictaluri, e. tarda, and e. piscicida). flavobacterium columnare and a. hydrophila formed thick biof ... | 2017 | 28406736 |
| the cc and cxc chemokine receptors in channel catfish (ictalurus punctatus) and their involvement in disease and hypoxia responses. | chemokines are vital regulators of cell mobilization for immune surveillance, inflammation, and development. chemokines signal through binding to their receptors that are a superfamily of seven-transmembrane domain g-coupled receptors. recently, a complete repertoire of both cc and cxc chemokines have been identified in channel catfish, but nothing is known about their receptors. in this study, a set of 29 cc chemokine receptor (ccr) genes and 8 cxc chemokine receptor (cxcr) genes were identifie ... | 2017 | 28842182 |
| first detection of edwardsiella ictaluri (proteobacteria: enterobacteriaceae) in wild australian catfish. | the bacterium edwardsiella ictaluri is considered to be one of the most significant pathogens of farmed catfish in the united states of america and has also caused mortalities in farmed and wild fishes in many other parts of the world. e. ictaluri is not believed to be present in wild fish populations in australia, although it has previously been detected in imported ornamental fishes held in quarantine facilities. in an attempt to confirm freedom from the bacterium in australian native fishes, ... | 2017 | 28836666 |
| effects of dietary tenebrio molitor meal on the growth performance, immune response and disease resistance of yellow catfish (pelteobagrus fulvidraco). | this study evaluated the influence of diets containing mealworm (tenebrio molitor) meal in partial substitution of fishmeal on growth performance and immune responses of juvenile yellow catfish (pelteobagrus fulvidraco). four diets were formulated to contain 0 (the control diet), 9, 18 and 27 g mealworm meal per 100 g diet with 0%, 25%, 50% and 75% of fishmeal replacement, respectively. yellow catfish were randomly divided into 4 groups with 3 replicates in each group. the fish in each group wer ... | 2017 | 28807649 |
| salinity stress, enhancing basal and induced immune responses in striped catfish pangasianodon hypophthalmus (sauvage). | in the mekong delta, striped catfish are faced with chronic salinity stress related to saltwater intrusion induced by global climatic changes. in this study, striped catfish juveniles were submitted to a prolonged salinity stress (up to 10ppt) over three weeks followed by infection with a virulent bacterial strain, edwardsiella ictaluri. osmoregulatory parameters were investigated. in addition, a label free quantitative proteomics workflow was performed on kidneys. the workflow consisted of an i ... | 2017 | 28797668 |
| complete genome sequence of edwardsiella ictaluri isolate rusvm-1 recovered from nile tilapia (oreochromis niloticus) in the western hemisphere. | edwardsiella ictaluri is a gram-negative bacillus that has recently been implicated in disease outbreaks in tilapia and zebrafish. we report here the complete and annotated genome sequence of an isolate from a nile tilapia (oreochromis niloticus), which contains a chromosome of 3,630,639 bp and two plasmids. | 2017 | 28619788 |
| the role of tonb gene in edwardsiella ictaluri virulence. | edwardsiella ictaluri is a gram-negative facultative intracellular pathogen that causes enteric septicemia in catfish (esc). stress factors including poor water quality, poor diet, rough handling, overcrowding, and water temperature fluctuations increase fish susceptibility to esc. the tonb energy transducing system (tonb-exbb-exbd) and tonb-dependent transporters of gram-negative bacteria support active transport of scarce resources including iron, an essential micronutrient for bacterial virul ... | 2017 | 29326601 |
| genetic studies to re-affiliate edwardsiella tarda fish isolates to edwardsiella piscicida and edwardsiella anguillarum species. | until 2012, the genus edwardsiella was composed by three species edwardsiella tarda, edwardsiella hoshinae and edwardsiella ictaluri. in 2013, edwardsiella piscicida, compiling fish pathogenic strains previously identified as e. tarda was described, and more recently a new species isolated from diseased eel was reported, namely edwardsiella anguillarum. the incorporation of these species into the genus makes necessary a revision of the taxonomic position of the isolates previously identified as ... | 2017 | 29150173 |
| improving safety of a live attenuated edwardsiella ictaluri vaccine against enteric septicemia of catfish and evaluation of efficacy. | edwardsiella ictaluri is a gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (esc). our recent work indicated that tricarboxylic acid cycle and one-carbon metabolism are critical pathways for e. ictaluri virulence. although single and double gene deletions in these pathways resulted in safe and efficacious vaccines for use in catfish fingerlings, vaccine trials in catfish fry showed safety concerns. therefore, we aimed to improve the safety of these m ... | 2017 | 29103702 |
| deciphering transcriptome profile of the yellow catfish (pelteobagrus fulvidraco) in response to edwardsiella ictaluri. | edwardsiella ictaluri is one of the most important pathogens posing a serious threat for yellow catfish (pelteobagrus fulvidraco), a highly valuable fish species of increasing commercial interest in china. here, a transcriptomic strategy was undertaken to investigate the yellow catfish gene expression profile against infection by the bacterial pathogen e. ictaluri. comparison of the transcriptome profiles between the infected and uninfected samples showed that a massive gene expression change oc ... | 2017 | 28866276 |
| the virulence and immune protection of edwardsiella ictaluri hemr mutants in catfish. | edwardsiella ictaluri is a gram-negative facultative intracellular rod, causing enteric septicemia of catfish (esc). several heme uptake systems have been described in bacterial pathogens, most of which involve outer membrane proteins (omps). we have shown recently that heme/hemoglobin receptor family protein (hemr) is significantly up-regulated in e. ictaluri under iron-restricted conditions. in this work, our goal was to construct e. ictaluri hemr mutants and assess their virulence and immune ... | 2018 | 29097322 |
| jak and stat members in channel catfish: identification, phylogenetic analysis and expression profiling after edwardsiella ictaluri infection. | the janus kinase/signal transducers and activators of transcription (jak/stat) signaling pathway is one of the main pleiotropic cascades used to transmit information from extracellular receptors to the nucleus, which results in dna transcription and expression of genes involved in immunity, proliferation, differentiation, migration, apoptosis, and cell survival. members of jak family and stat family have been extensively studied in different mammalian species because of their important roles in ... | 2018 | 29274790 |