Publications

biochemical characterization and 16s rrna sequencing of few lipase-producing thermophilic bacteria from taptapani hot water spring, orissa, india.	three lipase-producing thermophilic bacteria (ak-p1, ak-p2, and ak-p3) were isolated from the taptapani hot water spring in orissa, india. the crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture, and their lipolytic activities were compared. identification of the bacteria was carried out using biochemical tests, 16srrna sequencing and sequences submitted to ncbi genbank. strain ak-p3, exhibited the highest lipolytic activity of 5.5 u/ml was identi ...	2011	21541249
brevibacillus laterosporus strain bpm3, a potential biocontrol agent isolated from a natural hot water spring of assam, india.	a bacterial strain designated as bpm3 isolated from mud of a natural hot water spring of nambar wild life sanctuary, assam, india, strongly inhibited growth of phytopathogenic fungi (fusarium oxysporum f. sp. ciceri, f. semitectum, magnaporthe grisea and rhizoctonia oryzae) and gram-positive bacterium (staphylococcus aureus). the maximum growth and antagonistic activity was recorded at 30°c, ph 8.5 when starch and peptone were amended as carbon and nitrogen sources, respectively. in greenhouse e ...	2011	20630733
cloning, expression and purification of extracellular serine protease esp, a biofilm-degrading enzyme, from staphylococcus epidermidis.	staphylococcus epidermidis esp, an extracellular serine protease, inhibits staphylococcus aureus biofilm formation and nasal colonization. to further expand the biotechnological applications of esp, we developed a highly efficient and economic method for the purification of recombinant esp based on a brevibacillus choshinensis expression-secretion system.	2011	21974778
Purification and characterization of a serine protease secreted by Brevibacillus sp. KH3 for reducing waste activated sludge and biofilm formation.	A novel protease secreted by Brevibacillus sp. KH3 isolated from excess sludge at 50 °C and used as a sludge-lysing strain was investigated in this study. Sludge reduction was minimized by protease inhibitors and a 40-kDa protease, which significantly contributed to this sludge-reducing activity, was purified as the target protein. The final purified protease demonstrated 92-fold higher specific activity than the initial crude extracts. The sludge-reducing efficiency deteriorated relative to dec ...	2011	21925876
Genome sequence of Brevibacillus laterosporus LMG 15441, a pathogen of invertebrates.	Here we announce the genome sequence of the bacterium Brevibacillus laterosporus LMG 15441, which is a pathogen of invertebrates. The genome consists of one chromosome and two circular plasmids. Sequence analysis revealed a large potential to produce polyketides, nonribosomal peptides, and toxins.	2011	21914864
brevibacillus nitrificans sp. nov., a nitrifying bacterium isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks.	a heterotrophic nitrifying bacterium, designated as strain da2(t), was isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks. cells of strain da2(t) were gram-positive, facultatively anaerobic, motile by means of peritrichous flagella and sporulating rods, and grew in the ph range of 5-8. its major isoprenoid quinone was menaquinone-7 (mk-7), and its cellular fatty acid profile mainly consisted of iso-c15:0 (18.6%) and anteiso-c15:0 (69.1%). the dna ...	2011	22039003
a novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-o-methyltransferase.	membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. while large amounts of purified proteins are required for pharmaceutical and crystallization attempts, there is an unmet need for the development of novel heterologous membrane protein overexpression systems. specifically, we tested the application of brevibacillus choshinensis cells for the biosynthesis of human membrane bound catechol-o-methyltransferase (hmbcomt). in terms of the upstream stage mod ...	2011	21889552
comparing the tolerance limits of selected bacterial and protozoan species to nickel in wastewater systems.	heavy-metal resistant microorganisms play a significant role in the treatment of industrial wastewater. the detoxifying ability of these resistant microorganisms can be manipulated for bioremediation of heavy metals in wastewater systems. this study aimed at comparing the tolerance limit of selected wastewater protozoan species (aspidisca sp., trachelophyllum sp. and peranema sp.) against ni(2+) with that of selected bacterial species (bacillus licheniformis-atcc12759, brevibacillus laterosporus ...	2011	22014510
isolation of brevibacillus brevis from tracheal aspirates of a hospitalized patient.		2011	22085367
purification and characterization of a novel antimicrobial peptide from brevibacillus laterosporus strain a60.	a novel antimicrobial peptide, with molecular mass of 1602.0469da, produced by brevibacillus laterosporus strain a60 was isolated and purified from the soil of mango plants. the purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on an hitrap sp hp column, thin layer chromatography and high performance liquid chromatography (hplc) on c18 reversed-phase column. after the four isolation procedures, one peptide with antimicrobial activity was obtained ...	2012	22244810
Presence and potential role of thermophilic bacteria in temperate terrestrial environments.	Organic sulfur and nitrogen are major reservoirs of these elements in terrestrial systems, although their cycling remains to be fully understood. Both sulfur and nitrogen mineralization are directly related to microbial metabolism. Mesophiles and thermophiles were isolated from temperate environments. Thermophilic isolates were classified within the Firmicutes, belonging to the Geobacillus, Brevibacillus, and Ureibacillus genera, and showed optimum growth temperatures between 50°C and 60°C. Sulf ...	2012	22159635