Publications

brevibacillus nitrificans sp. nov., a nitrifying bacterium isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks.	a heterotrophic nitrifying bacterium, designated as strain da2(t), was isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks. cells of strain da2(t) were gram-positive, facultatively anaerobic, motile by means of peritrichous flagella and sporulating rods, and grew in the ph range of 5-8. its major isoprenoid quinone was menaquinone-7 (mk-7), and its cellular fatty acid profile mainly consisted of iso-c15:0 (18.6%) and anteiso-c15:0 (69.1%). the dna ...	2011	22039003
a novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-o-methyltransferase.	membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. while large amounts of purified proteins are required for pharmaceutical and crystallization attempts, there is an unmet need for the development of novel heterologous membrane protein overexpression systems. specifically, we tested the application of brevibacillus choshinensis cells for the biosynthesis of human membrane bound catechol-o-methyltransferase (hmbcomt). in terms of the upstream stage mod ...	2011	21889552
comparing the tolerance limits of selected bacterial and protozoan species to nickel in wastewater systems.	heavy-metal resistant microorganisms play a significant role in the treatment of industrial wastewater. the detoxifying ability of these resistant microorganisms can be manipulated for bioremediation of heavy metals in wastewater systems. this study aimed at comparing the tolerance limit of selected wastewater protozoan species (aspidisca sp., trachelophyllum sp. and peranema sp.) against ni(2+) with that of selected bacterial species (bacillus licheniformis-atcc12759, brevibacillus laterosporus ...	2011	22014510
isolation of brevibacillus brevis from tracheal aspirates of a hospitalized patient.		2011	22085367
diversity of endophytic bacteria within nodules of the sphaerophysa salsula in different regions of loess plateau in china.	a total of 115 endophytic bacteria were isolated from root nodules of the wild legume sphaerophysa salsula grown in two ecological regions of loess plateau in china. the genetic diversity and phylogeny of the strains were revealed by restriction fragment length polymorphism and sequencing of 16s rrna gene and enterobacterial repetitive intergenic consensus-pcr. their symbiotic capacity was checked by nodulation tests and analysis of nifh gene sequence. this is the first systematic study on endop ...	2011	21303396
involvement of residues asp8, asn13, glu145, asp168, and thr173 in the chaperone activity of a recombinant dnak from bacillus licheniformis.	based on the sequence homology, we have modeled the three-dimensional structure of bacillus licheniformis dnak (bldnak), a counterpart of hsp70, and identified five different amino acids that might be responsible for maintaining adp-mg(2+)-pi in the correct configuration at the atp-binding cleft of the protein. as compared with wild-type bldnak, site-directed mutant proteins d8a, n13d, e145a, d168a, and t173a had a dramatic reduction in their chaperone activities. complementation test revealed t ...	2011	21335977
comparative applications of azadirachtin- and brevibacillus laterosporus-based formulations for house fly management experiments in dairy farms.	the potential of two bioinsecticidal formulations containing brevibacillus laterosporus spores and azadirachtin, respectively, was assayed in laboratory and in comparative field treatments for the management of immature house flies on dairy farms. as already known for b. laterosporus, preliminary laboratory experiments with azadirachtin evidenced a concentration-dependent effect. azadirachtin median lethal concentration (lc50) value determined for second instar larvae was 24.5 microg/g diet. app ...	2011	21485372
cloning of a novel collagenase gene from the gram-negative bacterium grimontia (vibrio) hollisae 1706b and its efficient expression in brevibacillus choshinensis.	the collagenase gene was cloned from grimontia (vibrio) hollisae 1706b, and its complete nucleotide sequence was determined. nucleotide sequencing showed that the open reading frame was 2,301 bp in length and encoded an 84-kda protein of 767 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, the hexxh motif. g. hollisae collagenase showed 60 and 59% amino acid sequence identities to vibrio parahaemolyticus and v ...	2011	21515782
biochemical characterization and 16s rrna sequencing of few lipase-producing thermophilic bacteria from taptapani hot water spring, orissa, india.	three lipase-producing thermophilic bacteria (ak-p1, ak-p2, and ak-p3) were isolated from the taptapani hot water spring in orissa, india. the crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture, and their lipolytic activities were compared. identification of the bacteria was carried out using biochemical tests, 16srrna sequencing and sequences submitted to ncbi genbank. strain ak-p3, exhibited the highest lipolytic activity of 5.5 u/ml was identi ...	2011	21541249
purification and characterization of a novel antimicrobial peptide from brevibacillus laterosporus strain a60.	a novel antimicrobial peptide, with molecular mass of 1602.0469da, produced by brevibacillus laterosporus strain a60 was isolated and purified from the soil of mango plants. the purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on an hitrap sp hp column, thin layer chromatography and high performance liquid chromatography (hplc) on c18 reversed-phase column. after the four isolation procedures, one peptide with antimicrobial activity was obtained ...	2012	22244810
Presence and potential role of thermophilic bacteria in temperate terrestrial environments.	Organic sulfur and nitrogen are major reservoirs of these elements in terrestrial systems, although their cycling remains to be fully understood. Both sulfur and nitrogen mineralization are directly related to microbial metabolism. Mesophiles and thermophiles were isolated from temperate environments. Thermophilic isolates were classified within the Firmicutes, belonging to the Geobacillus, Brevibacillus, and Ureibacillus genera, and showed optimum growth temperatures between 50°C and 60°C. Sulf ...	2012	22159635