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use of waste chinese cabbage as a substrate for yeast biomass production.the possibility of using waste chinese cabbage (brassica campestris) as a substrate for microbial biomass production was investigated. the juice from waste chinese cabbage contains relatively high amounts of reducing sugars suitable for yeast culture. the cell mass and protein content of four species of yeast, candida utilis, pichia stipitis, kluyveromyces marxianus, and saccharomyces cerevisiae, were determined when cultured in juice extracted from cabbage waste. compared to ym broth containing ...200212094802
characterization of a stamen-specific cdna encoding a novel plant defensin in chinese cabbage.we isolated a stamen-specific cdna, bsd1 (brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in chinese cabbage (brassica campestris l. ssp. pekinensis). plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. in bsd1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. bsd ...200212139009
ban103, a pollen-preferential gene, from chinese cabbage and its promoter activity.we isolated and characterized a pollen-preferential gene, ban103, from chinese cabbage and analyzed the activity of its promoter. the ban103 cdna and genomic clone that contained the full-length gene were sequenced. the ban103 gene is a single copy in the chinese cabbage genome, and divided into three exons by two introns. the deduced sequence of 68 amino acids showed a homology with the brassica oleracea pollen coat protein, as well as several cold-induced proteins. ban103 transcription was res ...200212243345
structural variation and functional importance of a d-loop-t-loop interaction in valine-accepting trna-like structures of plant viral rnas.valine-accepting trna-like structures (tlss) are found at the 3' ends of the genomic rnas of most plant viruses belonging to the genera tymovirus, furovirus, pomovirus and pecluvirus, and of one tobamovirus species. sequence alignment of these tlss suggests the existence of a tertiary d-loop-t-loop interaction consisting of 2 bp, analogous to those in the elbow region of canonical trnas. the conserved g(18).psi(55) pair of regular trnas is found to covary in these tlss between g.u (possibly also ...200212364602
assessing copper thresholds for phytotoxicity and potential dietary toxicity in selected vegetable crops.copper pollution in soils is widespread, and its accumulation in crop products could pose a risk on human health. in this paper, bioavailability of added copper (cu) and critical cu concentrations in a vegetable garden soil was evaluated for chinese cabbage (brassica chinensis l.), pakchoi (brassica chinensis l.), and celery (apiumg graveolens l. var. dulce dc) based on human dietary toxicity. the availability of added cu in the soil decreased with incubation time, and had minimal change after 1 ...200212403270
isolation of pathogen-induced chinese cabbage genes by subtractive hybridization employing selective adaptor ligation.we have developed a subtractive cloning method in which target sequences are effectively enriched by selective adaptor ligation and pcr after hybridization. in this method both tester and driver dnas are digested with rsai, ligated with the linker dna containing a kpni recognition site, and amplified by pcr. the tester dna samples are divided into two aliquots, each digested with either rsai or kpni. the two dna samples are then combined and hybridized with an excess of the driver dna retaining ...200212445806
an outbreak of infections with a new salmonella phage type linked to a symptomatic food handler.in december 2001, the south australian communicable disease control branch investigated an outbreak of gastrointestinal illness linked to a korean style restaurant in metropolitan adelaide. twenty-eight people were identified as having experienced gastrointestinal symptoms subsequent to dining at the restaurant between 9 and 12 december 2001. a case-control study implicated mango pudding dessert (or 16.67 95% ci 2.03-177.04) and plain chicken (or 10.67 95% ci 1.04-264.32). nineteen cases and one ...200212549524
botanicals in cancer chemoprevention.botanicals have been used for the treatment of various human diseases throughout history. in addition, botanicals play a role in disease prevention. for example, epidemiologic studies have suggested that a reduced risk of cancer is associated with high consumption of vegetables and fruits. thus, the cancer chemopreventive potential of naturally occurring phytochemicals is of great interest. in this review, we discuss the cancer chemopreventive activity of cruciferous vegetables such as cabbage a ...200212549763
[type iii secretion system in se9r can recognize and secret harpin in erwinia amylovora].bacterial pathogens of both animals and plants use type iii secretion machines to secret virulence proteins. the signal that leads to tye type iii secretion is encoded as stem loop structure in the messenger rna. harpin, produced by erwinia anylovora is secreted via type iii secretion. erwinia carotovora se9r and erwinia amylovora belong to the same genus and they use type iii secretion machines to secret virulence proteins. we used pcr to amplify hrpn from pcpp430 which harbors hrpn gene cluste ...200212557554
comparative physiological responses in chinese cabbage induced by herbivory and fungal infection.fungal infection of chinese cabbage leaves by alternaria brassicae has earlier been shown to have detrimental effects on larval development of the chrysomelid beetle phaedon cochleariae. furthermore, adults of this leaf beetle avoid fungus-infected chinese cabbage leaves for oviposition and feeding. however, herbivory had no impact on fungal growth. in this study, we investigated physiological responses of the host plant to herbivore attack and fungal infection in order to elucidate the mechanis ...200212564792
indirect interactions between a phytopathogenic and an entomopathogenic fungus.slow growth in herbivores may lead to higher mortality, due to prolonged exposure to natural enemies. a number of studies has tested the 'slow-growth, high-mortality' hypothesis using predators or parasitoids as natural enemies of the herbivore, but the possible role of pathogens is poorly documented. we provide evidence that phytopathogenic infection of a plant enhances the susceptibility of herbivore larvae to a generalist entomopathogen. larvae of the mustard leaf beetle phaedon cochleariae g ...200312590299
stimulation by caffeic acid, coumalic acid, and corilagin of the germination of resting spores of the clubroot pathogen plasmodiophora brassicae.some chemicals were examined for their effects on the germination of resting spores of the clubroot pathogen plasmodiophora brassicae, and on the control of clubroots in chinese cabbage. caffeic acid, coumalic acid, and corilagin stimulated the germination of plasmodiophora spores and prevented the formation of clubroots in chinese cabbage. clubroot might be controlled by agents with germination-stimulating effects.200312619690
effects of cyazofamid against plasmodiophora brassicae woronin on chinese cabbage.cyazofamid (4-chloro-2-cyano-n,n-dimethyl-5-p-tolylimidazole-1-sulfonamide) is a novel fungicide with high levels of activity against oomycetes fungi and plasmodiophora brassicae woronin. the effects of cyazofamid were investigated against p. brassicae, the causal agent of clubroot disease in chinese cabbage. cyazofamid at 0.3 mg litre(-1) inhibited resting spore germination of this pathogen by about 80%. cyazofamid at 3-10 mg litre(-1) exhibited fungicidal activity to resting spores of p. brass ...200312639045
nitrate leaching in an andisol treated with different types of fertilizers.nitrate (no3) leaching was studied in an andisol treated with four n fertilizers (sc: swine compost, cu: coated urea, an: ammonium n, or nf: no fertilizer) for 7 years. sweet corn (zea mays l.) was grown in summer, followed by chinese cabbage (brassica rapa l. var. amplexicaulis) or cabbage (brassica oleracea l. var. capitata) in autumn each year. in chemical fertilizer plots treated with an or cu, no(3)-n concentrations in soil water at 1-m depth increased markedly in the summer of the second y ...200312685774
characterization of the xanthomonas axonopodis pv. glycines hrp pathogenicity island.we sequenced an approximately 29-kb region from xanthomonas axonopodis pv. glycines that contained the hrp type iii secretion system, and we characterized the genes in this region by tn3-gus mutagenesis and gene expression analyses. from the region, hrp (hypersensitive response and pathogenicity) and hrc (hrp and conserved) genes, which encode type iii secretion systems, and hpa (hrp-associated) genes were identified. the characteristics of the region, such as the presence of many virulence gene ...200312730176
peh production, flagellum synthesis, and virulence reduced in erwinia carotovora subsp. carotovora by mutation in a homologue of cytr.erwinia carotovora subsp. carotovora is a causal agent of soft-rot diseases in a wide variety of plants. here, we have isolated a new regulatory factor involved in the virulence of e. carotovora subsp. carotovora by in vivo insertional mutagenesis using a transposon tn5. the gene was homologous to cytr encoding a transcriptional repressor of nucleoside uptake and catabolism genes in escherichia coli, salmonella typhimurium, and vibrio cholerae. phenotypic characterization of a nonpolar deletion ...200312744509
growth conditions, elemental accumulation and induced physiological changes in chinese cabbage.soils contaminated with low levels of heavy metals and other trace elements are now frequently used for vegetable growing. in this situation, heavy metals and trace elements from these polluted soils may accumulate in the agricultural plants being grown in them and thereby enter the human food chain. the objectives of this study are to elucidate the effects of growth conditions, manipulated by the crop covers, on the phytoaccumulation of elements, and to investigate the conceivable influences of ...200312781236
characterization of salicylic acid-induced genes in chinese cabbage.salicylic acid is a messenger molecule in the activation of defense responses in plants. in this study, we isolated four cdna clones representing salicylic acid-induced genes in chinese cabbage (brassica rapa subsp. pekinensis) by subtractive hybridization. of the four clones, the bc5-2 clone encodes a putative glucosyltransferase protein. the bc5-3 clone is highly similar to an arabidopsis gene encoding a putative metal-binding farnesylated protein. the bc6-1 clone is a chitinase gene with simi ...200312835914
molecular detection and beta-glucuronidase expression of gus-marked bacillus subtilis l-form bacteria in developing chinese cabbage seedlings.to detect l-form bacteria in developing chinese cabbage seedlings.200312859751
urinary isothiocyanate levels, brassica, and human breast cancer.brassica vegetable consumption (e.g., chinese cabbage) provides isothiocyanates (itc) and other glucosinolate derivatives capable of inducing phase ii enzymes [e.g., glutathione s-transferases (gstm1, gstt1, and gstp1) and nadph quinine oxidoreductase] and apoptosis, altering steroid hormone metabolism, regulating estrogen receptor response, and stabilizing cellular proliferation. asian populations consuming large amounts of brassica have a lower breast cancer incidence compared with western pop ...200312873994
induction of male sterile cabbage using a tapetum-specific promoter from brassica campestris l. ssp. pekinensis.the anther (tapetum)-specific gene bca9 was isolated from chinese cabbage, brassica campestris l. ssp. pekinensis cv. jangwon, using the arabidopsis tapetum-specific a9 gene as a probe. the dna and amino acid sequences of the coding region of the bca9 gene showed high homology with a9 genes from arabidopsis and b. napus. however, the dna sequences of the 5' noncoding (promoter) region were different, except for the sequence from -281 to -89. to test the specific activity of this promoter, a plan ...200312920565
[chemical composition of volatile oil from chromolaena odorata and its effect on plant, fungi and insect growth].the volatile oil from c. odorata had a significant effect on the growth of plants, fungi and insects. the result showed that the seedling growth of five test plants was obviously inhibited, in the order of ryegrass > chinese cabbage > radish > snap been > rice. the inhibitory effect of the oil at its middle concentration (800 mg.l-1) for pyricularia grisea was the strongest, the next was to phytophthora nicotianae, and the weakest was to fusarium axysporum. the inhibitory percentage was 61.40%, ...200312924132
characterization of a salicylic acid- and pathogen-induced lipase-like gene in chinese cabbage.a cdna clone for a salicylic acid-induced gene in chinese cabbage (brassica rapa subsp. pekinensis) was isolated and characterized. the cabbage gene, designated br-sil1 (for brassica rapa salicylate-induced lipase-like 1 gene), encodes a putative lipase that has the family ii lipase motif gdsxxdxg around the active site serine. a database search showed that plant genomes have a large number of genes that contain the family ii lipase motif. the lipase-like proteins include a myrosinase-associated ...200314536025
[use of methyl parathion (mp)-degrading strain dll-e4 (pseudomonas sp.) to remove mp residue on the surface of agricultural products].in this paper, derivations from dll-e4, including fermented liquid, its centrifuged (3000 x g, 10 min) supernatant, pellet extract with supersonic, crude enzyme(supernatant and pellet extract), detergent, and mixture of detergent and pellet extract, were used to degrade the mp residue on the surface of chinese cabbage, tea and cucumber. the results showed that all of them were effective, with the best effect of 100%. some constituents in the supernatant of fermented liquid could stimulate the en ...200314986385
[induced activity of nitrate reductase by nitrate and cloning of nitrate reductase gene].excessive nitrate accumulated in plants affects vegetable quality severely and excessive nitrate ingestion would do harm to human health. assimilatory nadh: nitrate reductase (nr, ec 1.6.6.1), a complex mo-pterin-, cytochrome b(557)- and fad-containing protein, catalyzes the regulated and rate-limiting step in the utilization of inorganic nitrogen by higher plants. enhancing the activity of nr is conducive to reduce the concentration of nitrate in plants. the experiments were conducted to invest ...200315969098
decision making by generalist and specialist aphids with the same genotype.one idea to explain the high incidence of specialisation in phytophagous insects is that their nervous systems are simple and unable to efficiently recognise multiple host plants. here this 'neural limitations' hypothesis is tested using a generalist (the summer winged virginopara) and specialist (the autumn gynopara) morph of a single clone of the black bean aphid, aphis fabae, that are almost identical morphologically and have the same genotype. electrical monitoring of stylet behaviour and no ...200312837318
isolation and expression of cold-regulated cdna from chinese cabbage (brassica pekinensis).it has been established that changes in gene expression occur during cold acclimation in a wide range of plants. here we present a novel cdna encoding a protein with a calculated molecular mass of 25 kda, designated cor25, from brassica pekinensis cv. zaoshu 5 (chinese cabbage) that was similar with a cold-regulated arabidopsis thaliana l. gene, cor47. protein sequence alignment showed that cor25 had 53.5% of identity with cor47 and 59.6% of identity with erd10, an arabidopsis thaliana l. early- ...200314509836
survey of nitrate and nitrite contents of vegetables grown in korea.a scientific basis for the evaluation of the risk to public health arising from excessive dietary intake of nitrate in korea is provided. the nitrate () and nitrite () contents of various vegetables (chinese cabbage, radish, lettuce, spinach, soybean sprouts, onion, pumpkin, green onion, cucumber, potato, carrot, garlic, green pepper, cabbage and allium tuberosum roth known as crown daisy) are reported. six hundred samples of 15 vegetables cultivated during different seasons were analysed for ni ...200312888387
differential parasitism of plutella xylostella (lepidoptera: plutellidae) larvae by the parasitoid cotesia plutellae (hymenoptera: braconidae) on two host plant species.laboratory experiments were conducted to examine host selection by cotesia plutellae kurdjumov when larvae of its host, plutella xylostella (linnaeus), fed on chinese cabbage, brassica campestris l. ssp. pekinensis and those fed on common cabbage, brassica oleracea l. var. capitata were provided simultaneously, and to investigate the roles of plant and host volatiles in mediating host selection. when c. plutellae were provided with equal numbers of host larvae on plants of the two species in one ...200312593684
[effects of cruciferous vegetables on experimental population of diamondback moth plutella xylostella].the impact of four species of cruciferous vegetables on the experimental population of diamondback moth (dbm) plutella xylostella was evaluated in the laboratory. the results showed that there was no significant difference in total fecundity and longevity of dbm female moths reared on different crucifers. the population increase index of dbm reared on flowering chinese cabbage, chinese kale, leaf mustard, and pak-choi was 41.0, 69.5, 38.2 and 52.4, respectively, and the natural death at larvae s ...200314986376
comparative persistence of pesticides on selected cultivars of specialty vegetables.the behavior and dissipation of several pesticides on selected cultivars of specialty vegetable crops were compared to determine appropriate preharvest intervals for compliance with maximum residue limits. to reduce application variability, a tank mix of pesticides was applied for each trial. residues of eight pesticides applied to bok choi, chinese broccoli, and fuzzy squash were similar for the two leafy vegetables; residues were higher than on squash because of the latter's larger mass-to-sur ...200312590477
[mapping and analysis qtl controlling some morphological traits in chinese cabbage (brassica campestris l. ssp. pekinensis)].an aflp and rapd genetic map with 352 markers and a ril (recombinant inbred lines) population from the cross of two cultivated chinese cabbage lines were employed in mapping and analysis quantitative trait loci (qtl). the number, location, variation explained and additive effect of qtl underlying nine morphological traits were determined by using composite interval mapping method. fifty putative qtl, including five for plant growth habit, six for plant height, five for plant diameter, seven for ...200314986434
evaluation of the phytotoxicity of contaminated sediments deposited "on soil". i. impact of water draining from the deposit on the germination of neighbouring plants.as part of a study of the phytotoxic risk of spreading of contaminated sediments "on soil", we carried out a laboratory experiment assessing the impact of water draining from sediments in a deposit scenario on the peripheral vegetation. the plant tested were the chinese cabbage (brassica campestris l. var. chinensis), maize (zea mays l.) and ryegrass (lolium perenne l.). the draining water samples (a1, b1 and c1) were obtained after decantation in laboratory of the sediments. the classification ...200312656260
capacity and degree of iodine absorbed and enriched by vegetable from soil.to understand the biogeochemical transfer of iodine, the absorbability and bioaccumulation of iodine in tested vegetables (radish, spinach and chinese cabbage) are examined by applying iodic fertilizer composed of kelp and diatomaceous earth. the experimental results show that when iodine in soil is not excessive, the concentrations of iodine in tested vegetables increase as the content of iodine in soil increases. the absorbability and enrichment degree of iodine in various vegetables and in va ...200312602613
cruciferous vegetables, mushrooms, and gastrointestinal cancer risks in a multicenter, hospital-based case-control study in japan.we assessed the possible association of gastrointestinal cancers with cruciferous vegetables and mushrooms in a multicenter, hospital-based case-control study in an agricultural area of japan. one hundred forty-nine cases and 287 controls for stomach cancer and 115 cases and 230 controls for colorectal cancer were matched by age, sex, and residential area. in stomach cancer, the protective effect of vegetables (consumption of total vegetable) was obscure, but it became clearer when we examined s ...200314690789
development of a food composition database for the estimation of dietary intakes of glucosinolates, the biologically active constituents of cruciferous vegetables.evidence indicates that cruciferous vegetables are protective against a range of cancers with glucosinolates and their breakdown products considered the biologically active constituents. to date, epidemiological studies have not investigated the intakes of these constituents due to a lack of food composition databases. the aim of the present study was to develop a database for the glucosinolate content of cruciferous vegetables that can be used to quantify dietary exposure for use in epidemiolog ...200313129476
[structure and function of several intercropping ecosystems in newly built orchard].sloping wildland is the important potential land resource in china. structures and functions of five optimized models selected from more than 10 intercropping patterns in the newly built orchards in the uncultivated sloping field were analyzed in this paper. the results showed that organic matter was increased by 5-20%, total n by 7-40%, total p by 8-70%, and total k by 15-80% yearly. considering its benefit in soil and water conservation, the intercropping model of peanut and autumn soybean was ...200312920888
isolation of a napin-like polypeptide with potent translation-inhibitory activity from chinese cabbage (brassica parachinensis cv green-stalked) seeds.a heterodimeric napin-like polypeptide was isolated from brassica parachinensis seeds with a procedure involving ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, fplc-ion exchange chromatography on mono s and fplc-gel filtration on superdex 75. the n-terminal sequence of the 5 kda subunit of the polypeptide (pagpfripkkrkkee) showed high homology with other 2s storage proteins like napins and albumins. the polypeptide potently inhibited translation in a ...200312916641
biochemical and physiological changes in plants as a result of different sonic exposures.the effects of two different sonic exposures on two vegetables, namely chinese cabbage and cucumber at two growth stages, including seedlings and mature plants were investigated. the 3 h exposures included either 20 khz sound waves or "green music" that comprised classic music and natural sounds such as those of birds, insects, water, etc. analysis of variance between groups (anova) was used to determine the appropriate statistics parameters for the different treatments. both exposures caused si ...200312788223
developing a vitamin greenhouse for the life support system of the international space station and for future interplanetary missions.in order to evaluate the effects of gravity on growing plants, we conducted ground based long-term experiments with dwarf wheat, cultivar apogee and chinese cabbage, cultivar khibinskaya. the test crops had been grown in overhead position with hps lamp below root module so gravity and light intensity gradients had been in opposite direction. plants of the control crop grew in normal position under the same lamp. both crops were grown on porous metallic membranes with stable -1 kpa matric potenti ...200415846885
isolation and structures of acremolactones b and c, novel plant-growth inhibitory gamma-lactones from acremonium roseum i4267.novel acremolactones b and c were isolated from the acremolactone a-producing acremonium roseum i4267. the structure of acremolactone b having a phenylpyridyl gamma-lactone was elucidated by spectroscopic methods. it showed plant growth inhibitory activity toward chinese cabbage seedlings. the congener of acremolactone c having a phenylcyclopentenone gamma-lactone showed weak activity.200415618640
[investigation of pesticide residues in foods distributed in kitakyushu city].we investigated 160 kinds of pesticide residues in 715 samples of 116 kinds of foods distributed in kitakyushu city. sixty kinds of pesticides were detected in 55 kinds of foods (204 samples) in the range of 0.002-22 mg/kg. five kinds of pesticides in 7 samples violated the residue standards and the indication of "unused". the detection ratios of unregulated pesticide in domestic and imported foods were 27.8 and 33.0%, respectively. iprodione, dicofol, diethofencarb, procymidone and chlorfenapyr ...200415272606
prediction of phenanthrene uptake by plants with a partition-limited model.the performance of a partition-limited model on prediction of phenanthrene uptake by a wide variety of plant species was evaluated using a greenhouse study. the model predictions of root or shoot concentrations for tested plant species were all within an order of magnitude of the observed values. modeled root concentrations appeared to be more accurate than modeled shoot concentrations. the differences of simulated and experimented concentrations of phenanthrene in roots and shoots of three repr ...200415261414
changes in nitrate and nitrite content of four vegetables during storage at refrigerated and ambient temperatures.the nitrate and nitrite contents of four kinds of vegetables (spinach, crown daisy, organic chinese spinach and organic non-heading chinese cabbage) in taiwan were determined during storage at both refrigerated (5 +/- 1 degrees c) and ambient temperatures (22 +/- 1 degrees c) for 7 days. during storage at ambient temperature, nitrate levels in the vegetables dropped significantly from the third day while nitrite levels increased dramatically from the fourth day of storage. however, refrigerated ...200415204555
[component and content changes of volatiles from chinese cabbage damaged by plutella xylostella].the study showed that chinese cabbage, brassica campestris could release a variety of volatiles, especially when infested by plutella xylostella larvae. among these volatiles, saturated hydrocarbon was dominant, aromatic hydrocarbon was the second, and unsaturated hydrocarbon, aldehyde, alcohol, ketone, acid and heteroaromatic compounds were existed with a small amount. chinese cabbage damaged by plutella xylostella larvae produced 3 times of volatiles in amount with more species than the contro ...200415707333
molecular cloning and characterization of nucleoside diphosphate (ndp) kinases from chinese cabbage (brassica campestris).nucleoside diphosphate kinases (ndpks) are key metabolic enzymes that catalyze the synthesis of non-adenosine nucleoside triphosphates (ntp) by transfer of the terminal phosphate between ndp and ntp. recently we isolated three ndpk cdnas from chinese cabbage cdna library. bcndk1 has 636 bp and encodes a putative 17.4 kda protein, bcndk2 has 854 bp and encodes a putative 25.5 kda protein, and bcndk3 is 986 bp long and encodes a putative 25.7 kda protein. the precursor proteins of bcndk2 and bcndk ...200415055533
[the cytochemical observation of anthers of chinese cabbage's male-sterile].a chinese cabbage (brassica campestris l. ssp. chinensis makino) produces 1/4 male sterile and 3/4 fertility in offspring. the sterile plant can be identified from the color of corolla that is some white when it grows out. the fertile and sterile anthers were researched using cytological and cytochemical methods. thick sections of both anthers of different developmental stages were stained with toluidine blue for general cytological observation and stained with the periodic-acid-schiff's (pas) t ...200415511072
genotypic differences in effects of cadmium exposure on plant growth and contents of cadmium and elements in 14 cultivars of bai cai.fourteen cultivars of bai cai (brassica campestris l. ssp. chinensis var. communis) were grown in the nutrient solutions containing 0-0.5 microg ml(-1) of cadmium (cd) to investigate genotypic differences in the effects of cd exposure on the plant growth and uptake and distribution of cd in bai cai plants. the cd exposure significantly reduced the dry and fresh weights of roots and shoots, the dry weight ratio of shoot/root (s/r), total biomass, and chlorophyll content (spad value). cd concentra ...200415473646
[establishment of an efficient regeneration system for chinese cabbage cultured in vitro and histological observation of shoot development].an efficient plant regeneration system from explants of petiole with cotyledon was developed for chinese cabbage (brassica campestris ssp. chinensis makino) in our study. high frequency of shoot regeneration was obtained in the medium containing bap 2 mg/l, naa 0.45 mg/l and 5-7.5 mg/l agno3 solution to the half of nh4+ concentration's ms basic medium, with 84.3 percent being the highest frequency of shoot regeneration in seven chinese cabbage cultivars and one parentage, and the new formed shoo ...200415259989
a model for evaluation of the phytoavailability of trace elements to vegetables under the field conditions.currently, several single extraction methods are used for the evaluation of the phytoavailability of metals using pot experiments. a systematic comparison, however, is lacking. it is especially true for the field studies. this study was to investigate the phytoavailability of trace elements to vegetables grown on metal-contaminated soils under the field conditions. all soils collected were typical calcareous soils in northern china. four frequently used methods using cacl2, diethylenetriaminepen ...200415041285
adaptation of plants to altered shoot orientation relative to the gravity vector.wheat triticum aestivum l., carrots daucus carota l., chinese cabbage brassica pekinensis rupr., and african marigold tagetes patula l. were grown at natural and inverted orientation in the earth gravitational field. light vector was set unidirectional or opposite directional relative to the gravity vector. shoot orientation relative to the gravity vector was set natural or invert. plants grew in the special pots furnished with plane or cylindrical hydrophilic porous membranes. the membrane allo ...200416240510
[isolation and characterization of bcmf3, a gene expressed only in maintainer line in chinese cabbage-pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee)].expression profiling was performed using cdna-aflp technology on floral buds of a genic male sterile line (a line) and a maintainer line (b line) in chinese cabbage pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee cv. aijiaohuang). a differentially expressed cdna fragment, ba18-t16, was obtained via selective amplification with a18/t16 primer pair. ba18-t16 was specifically associated with the maintainer line, and was expressed only in floral buds revealed by half ...200415651684
a high-resolution karyotype of brassica rapa ssp. pekinensis revealed by pachytene analysis and multicolor fluorescence in situ hybridization.a molecular cytogenetic map of chinese cabbage (brassica rapa ssp. pekinensis, 2 n=20) was constructed based on the 4'-6-diamino-2-phenylindole dihydrochloride-stained mitotic metaphase and pachytene chromosomes and multicolor fluorescence in situ hybridization (mcfish), using three repetitive dna sequences, 5s rdna, 45s rdna, and c11-350h. the lengths of mitotic metaphase chromosomes ranged from 1.46 microm to 3.30 microm. five 45s and three 5s rdna loci identified were assigned to different ch ...200415365626
effects of se and zn supplementation on the antagonism against pb and cd in vegetables.the antagonistic effects of supplementation of zn and se to the soil on vegetables were studied in this work. in the pot experiment, se (se4+) and zn (zn2+) were applied, respectively, to the soil, in which the chinese cabbage (brassica rapa) and the lettuce (lactuca sativa l.) were planted. as a result, se and zn were enriched evidently in the two vegetables. the contents of pb and cd in the two vegetables were decreased markedly while contents of some healthy mineral elements, like mn and mg, ...200414749105
a novel locus for clubroot resistance in brassica rapa and its linkage markers.an inbred turnip ( brassica rapa syn. campestris) line, n-wmr-3, which carries the trait of clubroot resistance (cr) from a european turnip, milan white, was crossed with a clubroot-susceptible doubled haploid line, a9709. a segregating f(3) population was obtained by single-seed descent of f(2) plants and used for a genetic analysis. segregation of cr in the f(3) population suggested that cr is controlled by a major gene. two rapd markers, opc11-1 and opc11-2, were obtained as candidates of lin ...200414551685
a rac-like small g-protein from brassica campestris activates a pkc-dependent phospholipase d.a cdna clone encoding a rac-like small gtp binding protein was isolated from a cdna library of chinese cabbage (brassica campestris l. ssp. pekinensis) flower buds and named brac1. the brac1 cdna contains an open reading frame encoding 198 amino acid residues with an estimated molecular mass of 21,690 da and this coding region has conserved residues and motifs unique to the rho subfamily of proteins. the deduced amino acid sequence of the brac1 protein is closely related to that of arabidopsis t ...200414697272
the trypsin-inhibitory, immunostimulatory and antiproliferative activities of a napin-like polypeptide from chinese cabbage seeds.a heterodimeric 13.8 kda napin-like polypeptide has previously been isolated from chinese cabbage (brassica parachinensis) seeds with a procedure involving ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, fplc-ion exchange chromatography on mono s and fplc-gel filtration on superdex 75. in the present study the n-terminal sequence of the 8.8 kda subunit of the polypeptide (pqgpqqrppkllqqqtneehe) was found to have pronounced homology to napins, albumins ...200414994988
scar and caps mapping of crb, a gene conferring resistance to plasmodiophora brassicae in chinese cabbage ( brassica rapa ssp. pekinensis).clubroot disease, caused by plasmodiophora brassicae wor., is highly damaging for chinese cabbage. the cr (clubroot resistant) shinki dh (doubled haploid) line of chinese cabbage carries a single dominant gene, crb, which confers resistance to the p. brassicae races 2, 4, and 8. an f(2) population derived from a cross between the cr shinki dh line and a susceptible line, 94sk, was used to map the crb gene. inoculation of f(3) families with ssi (single-spore isolate) resulted in a 1:2:1 segregati ...200414997298
environmental concerns related to high thallium levels in soils and thallium uptake by plants in southwest guizhou, china.thallium (tl) contamination in soils poses a significant threat to human health due to the high toxicity of tl and its ready assimilation by crops. this study is focused on high concentrations of tl in soils in the lanmuchang area of southwest guizhou, china, which is related to natural processes of tl-rich sulfide mineralization. thallium contents range from 40 to 124 mg/kg in soils originating from the mining area, from 20 to 28 mg/kg in slope wash materials, from 14 to 62 mg/kg in alluvial de ...200414654287
allylnitrile: generation from cruciferous vegetables and behavioral effects on mice of repeated exposure.the objective of the present study was to examine the possible generation of allylnitrile from commonly consumed cruciferous vegetables, and to determine the long-term behavioral effects of its oral administration at levels comparable to or greater than human dietary exposures. on the basis of gaschromatographic and mass spectrometric analyses, allylnitrile generation was observed in eight out of twelve vegetables, broccoli, broccoli (young stems and leaves), brussels sprouts, cabbage, cauliflow ...200414871587
prevalence, antibiotic susceptibility, and virulence factors of yersinia enterocolitica and related species from ready-to-eat vegetables available in korea.a total of 673 ready-to-eat vegetable samples were collected in korea from 2001 to 2002 and analyzed for the presence of yersinia spp. we analyzed biotypes, serotypes, and susceptibility to 12 antibiotics and tested for virulence genes of pathogenic yersinia enterocolitica isolates by pcr assay. among the samples, 27 (4.0%) were found to be contaminated with yersinia spp. among the 27 strains of yersinia spp. isolates, 18 strains (66.7%) of y. enterocolitica, 5 strains (18.5%) of y. frederikseni ...200415222537
aerobic composting of chips from clear-cut trees with various co-materials.swollen chips made from trees felled during clear-cutting were composted with various organic and inorganic materials in an aerobic composting reactor for 5 months and then piled for 5 months. the organic materials included chicken feces, urea, nitrogenous lime (calcium cyanamide, manure), and material rapidly composted from food garbage in 24-h bacterial fermentation, while the inorganic materials were coal ash and volcanic ash. in this paper, we first attempt to estimate the quality and degree ...200415246435
cdna cloning and characterization of enolase from chinese cabbage, brassica campestris ssp. pekinensis.an enolase-encoding cdna clone from chinese cabbage, brassica campestris ssp. pekinensis, was isolated. this gene (accession number: ay307448) had a total length of 1580bp with an open reading frame of 1335bp, and encoded a predicted polypeptide of 444 amino acids with a molecular weight of 47.38 kda. the deduced amino acid (aa) sequence shared identity with a number of enolases ranging from bacillus subtilis to human beings and had much higher identity with other plant enolases than with enolas ...200415354355
microbial control of diamondback moth, plutella xylostella l. (lepidoptera: yponomeutidae) using bacteria (xenorhabdus nematophila) and its metabolites from the entomopathogenic nematode steinernema carpocapsae.cells and cell-free solutions of the culture filtrate of the bacterial symbiont, xenorhabdus nematophila taken from the entomopathogenic nematode steinernema carpocapsae in aqueous broth suspensions were lethal to larvae of the diamondback moth plutella xylostella. their application on leaves of chinese cabbage indicated that the cells can penetrate into the insects in the absence of the nematode vector. cell-free solutions containing metabolites were also proved as effective as bacterial cells ...200415362188
molecular characterization of a thij-like gene in chinese cabbage.a cdna clone for a salicylic acid-induced gene in chinese cabbage (brassica rapa subsp. pekinensis) was isolated and characterized. the cabbage gene encoding a protein of 392 amino acids contained a tandem array of two thij-like sequences. thij is a thiamin biosynthesis enzyme that catalyzes the phosphorylation of hydroxymethylpyrimidine (hmp) to hmp monophosphate. although the cabbage gene shows a similarity to bacterial thij genes, it also shares a similarity with the human dj-1, a multifuncti ...200415469717
laboratory evaluations of a wild crucifer barbarea vulgaris as a management tool for the diamondback moth plutella xylostella (lepidoptera: plutellidae).the term 'dead-end trap cropping' has recently been proposed to identify a plant that is highly attractive for oviposition by an insect pest, but on which offspring of the pest cannot survive. the potential of the wild crucifer barbarea vulgaris r. br. to allure and serve as a dead-end trap crop for the diamondback moth plutella xylostella (l.), an important pest of cruciferous crops worldwide, was examined in laboratory experiments. when p. xylostella adults were provided with a dual-choice of ...200415541190
[accumulation of coat protein of turnip mosaic virus in host chloroplasts and its effect on ps ii activity].intact chloroplasts were isolated from chinese cabbage (brassica chinensis l. cv. suzhou) and mustard (b. juncea l. cv. wenzhou) leaves inoculated with turnip mosaic virus (tumv). the proteins attached to the surface of chloroplasts were digested, then the total proteins of chloroplasts were extracted. by analysis of the sds-page pattern, one extra protein band with the same mobility and molecular weight as tumv-coat protein was found in the chloroplasts of infected leaves. results of western bl ...200415583406
turnip yellow mosaic virus: transfer rna mimicry, chloroplasts and a c-rich genome.summary taxonomy: turnip yellow mosaic virus (tymv) is the type species of the genus tymovirus, family tymoviridae. tymv is a positive strand rna virus of the alphavirus-like supergroup. physical properties: virions are non-enveloped 28-nm t = 3 icosahedrons composed of a single 20-kda coat protein that is clustered in 20 hexameric and 12 pentameric subunits. infectious particles and empty capsids coexist in infected tissue. the genomic rna is 6.3 kb long, with a 5'(m7)gpppg cap and a 3' untrans ...200420565613
control of verticillium yellows in chinese cabbage by the dark septate endophytic fungus ltvb3.abstract three hundred forty-nine fungal endophytes were obtained from a total of 1,214 root segments of eggplant, melon, barley, and chinese cabbage grown as bait plants in a mixed soil made up of samples from different forest soils in alberta and british columbia, canada. three of the 349 isolates, when inoculated in axenically reared chinese cabbage seedlings grown in petri dishes, almost completely suppressed the effects of a postinoculated and virulent strain of verticillium longisporum. tw ...200418943758
efficacy of acidified sodium chlorite treatments in reducing escherichia coli o157:h7 on chinese cabbage.efficacy of acidified sodium chlorite for reducing the population of escherichia coli o157:h7 pathogens on chinese cabbage leaves was evaluated. washing leaves with distilled water could reduce the population of e. coli o157:h7 by approximately 1.0 log cfu/g, whereas treating with acidified chlorite solution could reduce the population by 3.0 log cfu/g without changing the leaf color. a similar level of reduction was achieved by washing with sodium chlorite solution containing various organic ac ...200515726965
infection of chinese cabbage by plasmodiophora brassicae leads to a stimulation of plant growth: impacts on cell wall metabolism and hormone balance.the importance of plant hormones in clubroot infection has long been recognized. the morphological changes, such as cell division and cell elongation leading to gall formation are triggered in the early stages of infection. we analysed cell expansion by localizing xyloglucan endotransglucosylase/hydrolase (xth)-action and screened the endogenous concentrations of several classes of phytohormones by mass spectrometry in the early stages of plasmodiophora brassicae infection in chinese cabbage (br ...200515760367
copper toxicity and bioaccumulation in chinese cabbage (brassica pekinensis rupr.).copper is among the major heavy metal contaminants in the environment with various anthropogenic and natural sources. human health risk from heavy metal bioaccumulation in vegetables has been a subject of growing concern in recent years. to investigate cu phytotoxic effects and bioaccumulation in the popular vegetable chinese cabbage (brassica pekinensis rupr) as well as the implications for human health due to cu in the vegetable supply, seed germination and pot culture experiments with this ve ...200515793819
[distribution of rubisco and rca in brassica chinensis chloroplasts and effect of tumv-infection on their cellular localization].the cellular localizations of rubisco and rubisco activase (rca) in chinese cabbage (brassica chinensis l. cv. suzhou) leaves were investigated by immunogold-labeling electron microscopy. the results showed that rubisco and rca were mainly located in chloroplasts of mesophyll, guard cell of stomatal apparatus and parenchyma of vascular bundle. a high density of gold particles were localized preferentially to the chloroplast stroma. in contrast, there were no specific binding of gold particles de ...200515839204
novel reference gene, high-mobility-group protein i/y, used in qualitative and real-time quantitative polymerase chain reaction detection of transgenic rapeseed cultivars.with the development of transgenic crops, regulations to label the genetically modified organisms (gmos) and their derived products have been issued in many countries. polymerase chain reaction (pcr) methods are thought to be reliable and useful techniques for qualitative and quantitative detection of gmos. these methods are generally needed to amplify the transgene and compare the amplified results with that of a corresponding reference gene to get the reliable results. specific primers were de ...200515859086
prewashing with acidified sodium chlorite reduces pathogenic bacteria in lightly fermented chinese cabbage.efficacy of prewashing with acidified sodium chlorite (asc) for the sanitation of lightly fermented chinese cabbage was evaluated. the population of the natural microflora on the cabbage leaves was reduced about 2.0 log cfu/g just after washing with asc, a significant reduction compared with the control distilled water wash (p < or = 0.05). in the control experiment, viable aerobic bacteria increased gradually when incubated at 10 degrees c; however, asc-washed cabbage maintained a lower microbi ...200515895733
comparative study of mycelial growth and basidiomata formation in seven different species of the edible mushroom genus hericium.the potential of using several agricultural by-products as supplements of sawdust substrate for the production of edible mushroom hericium was evaluated using seven hericium species. all the tested supplements (rice bran, wheat bran, barley bran, chinese cabbage, egg shell, and soybean powder) were found to be suitable for the mycelial growth of all the tested species. in mycelial growth, soybean powder was the best supplement for hericium americanum, hericium coralloides, and hericium erinaceum ...200515939270
sulfur, chromium, and selenium accumulated in chinese cabbage under direct covers.currently, pollution of our agricultural soils and waters is increasing and is often associated with many human health ailments. soils contaminated with low levels of heavy metals and other trace elements are frequently used for growing vegetable crops and in such a situation, these toxic contaminants often accumulate in the edible portions of these agricultural plants and thereby enter the human food chain. in 3 consecutive years of field experiments (1994-1996), two different crop-covering tre ...200515572085
tissue-dependent distribution and accumulation of chlorobenzenes by vegetables in urban area.five seasonal vegetables from three growing sites in hangzhou city, zhejiang province, were studied for the levels of four chlorobenzenes(cbs): o-dichlorobenzene (o-dcb), p-dichlorobenzene (p-dcb), m-dichlorobenzene (m-dcb), and 1,2,4-trichlorobenzene (1,2,4-tcb). samples of each vegetable from each site were subdivided into leaves, stems, and roots, and these subsamples were analyzed separately for the levels of accumulated cbs. relations between the levels of cbs in vegetables with the total o ...200516002141
genes and environment interact to determine the fitness costs of resistance to bacillus thuringiensis.genes which provide resistance to novel challenges such as pesticides, toxins or pathogens often impose fitness costs on individuals with a resistant phenotype. studies of resistance to bacillus thuringiensis and its insecticidal cry toxins indicate that fitness costs may be variable and cryptic. using two field populations (karak and serd4) of the diamondback moth, plutella xylostella, we tested the hypothesis that the costs associated with resistance to the b. thuringiensis toxin cry1ac would ...200516011928
bacterial community structure in kimchi, a korean fermented vegetable food, as revealed by 16s rrna gene analysis.kimchi is a traditional korean food fermented from a variety of vegetables. we elucidated the microbial community structure of five commercially produced kimchis made from chinese cabbage by examining culture-independent 16s rrna gene clone libraries. most of the clones (347 out of 348) belonged to lactic acid bacteria and included several species of the genera lactobacillus, leuconostoc and weissella. weissella koreensis was found in all the samples and predominated in three of them (42.6-82%). ...200516084269
elevated temperature enhances virulence of erwinia carotovora subsp. carotovora strain ec153 to plants and stimulates production of the quorum sensing signal, n-acyl homoserine lactone, and extracellular proteins.erwinia carotovora subsp. atroseptica, e. carotovora subsp. betavasculorum, and e. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (pel), polygalacturonase (peh), cellulase (cel), and protease (prt), and the quorum-sensing signal n-acyl-homoserine lactone (ahl) at 28 degrees c. however, the production of these enzymes and ahl by these bacteria is severely inhibited during growth at elevated temperatures (31.2 degrees c for e. carotovora subsp. atr ...200516085860
[effects of colletotrichum higginsianum infection on endogenous hormones in brassica parachinensis leaves under different nitrogen nutrition].this paper studied the effects of colletotrichum higginsianum infection on the ethylene production and the contents of abscisic acid (aba) and indole-3-acetic acid (iaa) in leaves of flowering chinese cabbage (brassica parachinensis) under six levels of nitrogen (n) nutrition, and their relations to disease-resistance. the results showed that the ethylene production in all n treatments had a mono-peak curve change, and reached the maximum 4-6 days after inoculation. in comparing with high n or n ...200516110672
characterization of a pollen-preferential gene, ban102, from chinese cabbage.we isolated and characterized a pollen-preferential gene, ban102, from chinese cabbage and analyzed the activity of its promoter. there were three or four copies of the ban102 gene in the chinese cabbage genome that specifically expressed in pollen and pollen tube. there were 2137 bp of ban102 genomic clone comprising 186 bp of protein coding region, and 1178 bp of 5' and 773 bp of 3' non-coding regions. tata box were located at 1071 nt of the promoter region while the polyadenylation signal and ...200516160834
the development and endophytic nature of the fungus heteroconium chaetospira.the root endophytic fungus heteroconium chaetospira was isolated from roots of chinese cabbage grown in field soil in japan. this fungus penetrates through the outer epidermal cells of its host, passes into the inner cortex, and grows throughout the cortical cells, including those of the root tip region, without causing apparent pathogenic symptoms. there are no ultrastructural signs of host resistance responses. h. chaetospira has been recovered from 19 plant species in which there was no disru ...200516168582
chinese cabbage extracts and sulforaphane can protect h2o2-induced inhibition of gap junctional intercellular communication through the inactivation of erk1/2 and p38 map kinases.the cruciferous vegetables such as chinese cabbages and broccoli are known to have anticancer phytochemicals, and the consumption of cruciferous vegetables has been proposed to protect against various cancers. the anticarcinogenic properties of some chinese cabbage extracts and sulforaphane glucosinolate (sfn) were assessed by examining their ability to prevent the inhibition of gap junctional intercellular communication (gjic) induced by hydrogen peroxide (h2o2) in wb-f344 normal rat liver epit ...200516218665
over-expression of chinese cabbage calreticulin 1, brcrt1, enhances shoot and root regeneration, but retards plant growth in transgenic tobacco.calreticulin (crt) is a ubiquitously expressed, high capacity ca(2+)-binding protein that is involved in intracellular ca(2+) homeostasis and molecular chaperoning in the endoplasmic reticulum (er). a cdna encoding a calreticulin, brcrt1 (brassica rapa calreticulin 1), has been isolated from chinese cabbage (b. rapa subsp. pekinensis) flower bud. constitutive over-expression of the brcrt1 gene promotes robust shoot production and root formation at sub-optimal concentrations of ba/naa, which are ...200516245153
[the distribution and viability changes of the agrobacteria in the vacuum infiltrated plants of chinese cabbage].to understand further the mechanism of agrobacteria transformation by vacuum infiltration, the distribution and viability changes of the agrobacteria in infiltrated chinese cabbage( b. rapa ssp. chinesis) plants were investigated by histochemical staining and bacteria plating techniques. results revealed that there were different quantities of agrobacteria in different organs, among which the most quantity occurred in flowers, especially in ovules. the agrobacteria lied in the space between cell ...200516245885
activation of defense responses in chinese cabbage by a nonhost pathogen, pseudomonas syringae pv. tomato.pseudomonas syringae pv. tomato (pst) causes a bacterial speck disease in tomato and arabidopsis. in chinese cabbage, in which host-pathogen interactions are not well understood, pst does not cause disease but rather elicits a hypersensitive response. pst induces localized cell death and h2o2 accumulation, a typical hypersensitive response, in infiltrated cabbage leaves. pre-inoculation with pst was found to induce resistance to erwinia carotovora subsp. carotovora, a pathogen that causes soft r ...200516336791
[development of est (expressed sequence tags) marker in chinese cabbage and its transferability to repeseed.].28 pairs of primers were designed according to the expressed sequence tags in chinese cabbage. after testing on the annealing temperature and the concentration of primer, dntp and mgcl2, a suitable pcr system was established. under the condition of reaction system developed, primers designed specific to ests were screened against genomic dna of inbreed line a from which the cdna library was constructed. among them, 18 pairs of primers showed the amplification. then all the primers available in l ...200515985406
[differences of gene expression in bud stage of backcross hybrid between ogura-type male-sterile brassica napus l and b campestris l versus parents].crosses between female parent of ogura male sterility brassica napus l. and male parents of b. campestris ssp. chinensis makino were made and f(1), bc(1) and bc(2) generations produced. gene expression of two chinese cabbage backcross hybrid bc(1), bc(2) and their parents at bud stage was analyzed by means of cdna-aflp technique. the results indicated that the patterns of gene expression differ significantly between bc(1) and bc(2) generations and their parents. there were many patterns of gene ...200515843356
physical mapping and microsynteny of brassica rapa ssp. pekinensis genome corresponding to a 222 kbp gene-rich region of arabidopsis chromosome 4 and partially duplicated on chromosome 5.we constructed a bacterial artificial chromosome (bac) library, designated as kbrh, from high molecular weight genomic dna of brassica rapa ssp. pekinensis (chinese cabbage). this library, which was constructed using hindiii-cleaved genomic dna, consists of 56,592 clones with average insert size of 115 kbp. using a partially duplicated dna sequence of arabidopsis, represented by 19 and 9 predicted genes on chromosome 4 and 5, respectively, and bac clones from the kbrh library, we studied conserv ...200516283385
allelopathic potential of robinia pseudo-acacia l.robinia pseudo-acacia l. (black locust) is a nonindigenous species currently invading the central part of japanese grasslands. several allelochemicals were identified and characterized from the leaf tissue. the growth of both radicle and hypocotyl in the tested species (barnyard grass, white clover, lettuce, and chinese cabbage) was reduced when grown in soil mixed with the leaves of r. pseudo-acacia at various concentrations. aqueous leaf extracts, when bioassayed, exhibited a significant suppr ...200516132219
[effects of environmental factors on phyllotreta striolata dispersion].using marking recapture method, this paper studied the effects of host plants and climatic factors on the dispersion of phyllotreta striolata adults. the results showed that the dispersion of the beetle was impelled by environment elements (tao = 1 ). temperature and wind speed had significantly positive correlations with the dispersion of the beetle (p < 0.05), while high temperature had a negative correlation. wind orientation had a significant effect on the dispersion of the beetle, with the ...200516180758
[the ultrastructural observation of anthers of chinese cabbage's mail-sterility].the fertile and sterile anthers of a chinese cabbage (brassica campestris l. ssp. chinensis makino) were observed using electron microscope to find the ultrastructural feature of sterile anthers. the earliest abnormal phenomenon in sterile anther was nucleolus of sporogenous cells locating in the edge of nucleus. during microspore mother cell development, callus wall surrounding the cell displayed uneven in the thick ness and was discontinuous,and the some cytoplasm leaked out of the cell from s ...200516416967
[calcium distribution in fertile and sterile anthers of a genic male sterile chinese cabbage].potassium antimonite was used to locate calcium in the fertile and sterile anthers of a genic male sterile chinese cabbage (brassica campestris l. ssp. chinensis makino) to probe the relation between ca(2+) and fertility and sterility of anthers of the cabbage. during fertile anther development, calcium granules increase in number in anther wall cells after meiosis, and then appeared also in locule, suggesting a calcium influx into locule from anther wall cells (plate i-4). then the number of ca ...200516361789
phytotoxicity assessment of phenanthrene, pyrene and their mixtures by a soil-based seedling emergence test.seedling emergence tests were conducted in a meadow brown soil using five plant species (i.e., chinese cabbage, green onion, tomato, turnip and wheat) to determine the phytotoxicity of phenanthrene, pyrene and their mixtures. the soil was amended with up to 1000 mg/kg soil of phenanthrene or 600 mg/kg soil of pyrene. seedling emergence and root growth were measured. the results indicated that root elongation was more sensitive than seedling emergence. root length decreased with increasing phenan ...200516158583
enzyme immunoassay for the determination of carbaryl residues in agricultural products.carbaryl is one of the most important n-methyl carbamate insecticides used all over the world. to determine carbaryl residues in agricultural products (apple, pear, chinese cabbage, rice and barley), a competitive enzyme-linked immunosorbent assay (elisa) method was employed, and a simple and efficient extraction method for the rapid detection of carbaryl in agricultural products was developed with recoveries greater than 90%. after liquid extraction, sample extracts diluted with buffer were ana ...200516147429
dietary habits and risk of urothelial cancer incidence in the jacc study.the relationships between dietary habits and urothelial cancer have been discussed in many epidemiologic studies, however, they have not been sufficiently elucidated. in the present study, the associations of dietary habits with the risk of urothelial cancer incidence were evaluated taking into consideration sex, age, and smoking habits.200516127233
isotope characteristics of vegetables and wheat from conventional and organic production.multielement isotope ratio analysis was checked for its suitability as a means for the discrimination between agricultural products from integrated/conventional or organic production, respectively. differences were mainly found for delta15n-values. paprika and tomatoes from organic production in greenhouses showed delta15n-values above+7 per thousand, whereas corresponding products from conventional cultivation had delta15n-values near 0 per thousand. lettuce, onions, cabbage and chinese cabbage ...200516126518
joint effects of acetochlor and urea on germinating characteristics of crop seeds.in order to evaluate ecological risk of agrochemicals in common use, joint toxic effects of acetochlor and urea on germinating characteristics of chinese cabbage (brassica pekinensis rupr) seeds were investigated using the water-culture method and the soil-culture method. the results indicated that excessive application of acetochlor and urea, when the concentrations were higher than 31.3 mg x kg(-1) for acetochlor and 500 mg x kg(-1) for urea, had strong inhibitory effects on the rate of seed g ...200516089323
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