Publications

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tinea capitis in cleveland: survey of elementary school students.tinea capitis, a fungal infection of the scalp, is of increasing public health importance, and trichophyton tonsurans has become the primary causative agent in north america.200312582387
tinea capitis in adult women masquerading as bacterial pyoderma.tinea capitis is generally thought to be a common disease in children but not in adults. adults with tinea capitis generally present with scale and alopecia. we report 3 adults with inflammatory tinea capitis caused by trichophyton tonsurans that resembled a bacterial infection. of these patients, 2 were initially given a diagnosis of bacterial pyoderma. all patients were successfully treated with oral antifungal agents. one patient had significant eosinophilia that resolved with treatment. we c ...200312894115
genetic heterogeneity in the rrna gene locus of trichophyton tonsurans.trichophyton tonsurans is the major pediatric pathogen in tinea capitis, causing disparate disease presentations. little is known about genetic variation, which may ultimately be linked to divergent disease status. this investigation was aimed at identifying genetic variants of t. tonsurans by methods that can facilitate strain discrimination in population-based studies. ninety-two isolates were acquired from six u.s. microbiology laboratories, and genomic dna was isolated from mature colonies. ...200314662928
[a case of tinea capitis caused by trichophyton tonsurans].a 10-year-old peruvian girl, living in japan since 1996, visited our hospital in august 2000 complaining of alopecia which had been present on her scalp for one year. the bald areas appeared as multiple small, scattered, angular patches with indistinct margins. follicular pustules, erythemic nodules and lymphadenopathy were also seen. in the culture of the affected hair, a tan surface with wiry undulations grew on sabouraud's media. the colony reverse had reddish-brown central pigmentation. slid ...200312590256
in vitro antifungal activity of sertaconazole against 309 dermatophyte clinical isolates.three hundred and nine strains belonging to 11 species of dermatophyte moulds were tested against sertaconazole following mainly the national committee for clinical laboratory standards (m38-p) for filamentous fungi. however, several important factors such as the temperature (28 degrees c vs 35 degrees c) and time of incubation (4-10 d vs 21-74 h), have been modified. sertaconazole was active against all the clinically important dermatophyte moulds involved in human infections tested. overall ge ...200314998079
[tinea capitis in children from goiânia, brazil].during the period january 1999 to july 2002 a total de 164 cases of tinea capitis were diagnosed by mycological examination in instituto de patologia tropical e saúde pública of the universidade federal de goiás. of the 164 patients 94 (57.3%) were males, with an age of 3 months to 13 years. laboratory studies were performed by direct examination with 20% koh and cultivated on mycobiotic agar medium and sabouraud dextrose agar with chloramphenicol. the following species were identified: microspo ...200315049102
facial majocchi's granuloma caused by trichophyton tonsurans in an immunocompetent patient. 200312636033
tinea capitis in brussels: epidemiology and new management strategy.we found pre-established directives inadequate to cope with the current increase in anthropophilic tinea capitis in brussels.200312771493
occurrence of dermatomycoses and in-vitro therapeutic efficacy of three antifungal drugs on the growth of epidermophyton floccosum.the occurrence of dermatomycoses and the in-vitro therapeutic efficacy of some antifungal agents on dermatomycotic organisms were investigated. of the 550 primary school children screened, the incidence was one hundred (18%), 70 were males (representing 20% of the males screened) and 30 females (15% of the females sampled). the differences between male and female prevalence were insignificant. three species of dermatophytes were isolated and identified. these were microsporum canis, trichophyton ...200314682454
tinea barbae (tinea sycosis): experience with nine cases.tinea barbae is a rare dermatophytosis that affects the hair and hair follicles of the beard and mustache. this paper presents 9 cases of tinea barbae observed over an 18-year period of time and classified as follows: 1 was superficial and 8 were deep (6 folliculitis-like and 2 kerion-like). most of the cases (4) were associated with topical steroid therapy, others with pet contact (3 cases) and one with diabetes. the causal agents isolated were: trichophyton rubrum in 3; microsporum canis in 3; ...200314739517
tinea capitis among children and adolescents in the farwaniya region of kuwait.analysis of 325 patients (182 males, 143 females) of mycologically proven tinea capitis (tc) seen over a period of two years from january 2001--december 2002 in the farwaniya region of kuwait is presented in this study. the age range was 8 months to 17 years. peak incidence was observed in the 3-14 year age group (79.6%). positive family history and contact with pets were noted in 22% and 36.7% of the cases, respectively. the non-inflammatory 'gray patch' variety was the most common clinical typ ...200314739518
dermatophyte agents in the city of são paulo, from 1992 to 2002.dermatophytosis are superficial mycoses caused by fungi that can invade stratum corneum and keratinized tissues. in order to study the frequency of dermatophytes species and the clinical manifestations caused by these fungi, in são paulo, sp, brazil, the authors analyzed cultures isolated at the mycology laboratory from a selected population (15,300 out-patients of the hospital das clínicas, department of dermatology, faculty of medicine of university of são paulo) from january 1992 to june 2002 ...200314743665
rapid identification of trichophyton tonsurans by pcr-rflp analysis of ribosomal dna regions.culture morphology of trichophyton (t.) tonsurans, an emerging pathogen of dermatophytosis in japan, varies widely and species level identification is sometimes very difficult. reliable molecular markers are expected to be introduced for their identification.200312788526
antimicrobial and antioxidant activities of three mentha species essential oils.the present study describes the antimicrobial activity and free radical scavenging capacity (rsc) of essential oils from mentha aquatica l., mentha longifolia l., and mentha piperita l. the chemical profile of each essential oil was determined by gc-ms and tlc. all essential oils exhibited very strong antibacterial activity, in particularly against esherichia coli strains. the most powerful was m. piperita essential oil, especially towards multiresistant strain of shigella sonei and micrococcus ...200312802721
scalp ringworm in south-east london and an analysis of a cohort of patients from a paediatric dermatology department.scalp ringworm or tinea capitis has become an increasingly important public health issue in the past decade in great britain. recently, certain dermatology departments in london have seen a large increase in tinea capitis in all its forms.200312786830
clinico-mycological profile of superficial mycosis in a hospital in north-east india.a clinico-mycological study of superficial mycoses was conducted on 121 cases (98 males and 23 females). direct microscopy by koh mount and culture was undertaken to isolate the fungal pathogen in each case. the commonest age group involved was 21-30 years (39.6%). dermatophytosis was the commonest clinical presentation (70.5%), followed by candidiasis (20.5%) and pityriasis versicolor (9.0%). the commonest dermatophytosis was tinea pedis (29.2%), followed by t cruris (26.2%). the commonest derm ...200327407482
pcr and pcr-rflp techniques targeting the dna topoisomerase ii gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis.we have focused on the dna topoisomerase ii genes of several pathogenic fungi, and developed polymerase chain reaction (pcr) and pcr-restriction fragment length polymorphism (rflp) methods targeting this gene for identification of dermatophytes.200414757280
trichophyton rubrum tinea capitis in a young child.while the primary etiologic agent of tinea capitis in the united states has varied over the past century, the last several decades have been marked by a predominance of infection with trichophyton tonsurans. related anthropophilic organisms commonly isolated in other dermatophytoses are infrequently observed in tinea capitis. we report the unusual occurrence of tinea capitis in a young boy infected with trichophyton rubrum.200414871330
therapeutic options in the treatment of tinea capitis.tinea capitis is primarily a disease of pre-adolescent children. in north america and the uk, trichophyton tonsurans is responsible for > 90% of cases. microsporum canis is the predominant pathogen in certain parts of europe. the standard of care for the treatment of tinea capitis is oral griseofulvin and so far, it remains the only medication approved by the us fda for this condition. the newer oral antifungal agents, such as terbinafine, itraconazole and fluconazole, appear to be effective, sa ...200414996619
intra- and interlaboratory study of a method for testing the antifungal susceptibilities of dermatophytes.the national committee for clinical laboratory standards (nccls) m38-a standard for the susceptibility testing of conidium-forming filamentous fungi does not explicitly address the testing of dermatophytes. this multicenter study, involving six laboratories, investigated the mic reproducibility of seven antifungal agents tested against 25 dermatophyte isolates (5 blinded pairs of five dermatophyte species per site for a total of 300 tests), using the method of dermatophyte testing developed at t ...200415243047
tinea capitis in a dermatology center in the city of fortaleza, brazil: the role of trichophyton tonsurans.over a 3-year period (march 1999 to march 2002), 944 patients with scalp lesions attended a dermatology reference center in the city of fortaleza, ceará, brazil. clinical specimens were examined at the specialized medical mycology center, federal university of ceará, fortaleza-ce, brazil, to detect patients with tinea capitis.200415304180
new treatments for tinea capitis.tinea capitis, a dermatophyte infection involving the hair shaft on the scalp, is primarily a disease of preadolescent children. the predominant pathogen varies according to the geographical location. trichophyton tonsurans and microsporum canis account for the majority of infections in north america and certain parts of europe. the current standard of care for the treatment of tinea capitis in the usa is oral griseofulvin, but evidence is accumulating that some of the newer antifungal agents ma ...200415021047
[screening examination and management of dermatophytosis by trichophyton tonsurans in the judo club of a university].thirty-one members of the judo club of a certain university (age: 18~23) underwent a screening examination for dermatophytosis by trichophyton tonsurans. test items were: age, sex, height, weight, living mode, exercise duration, number of judo contestants, presence of foreign contestants, occurrence, if any, of dermatophytosis past or present according to a subject's answers to a questionnaire, medical examinations and mycological examinations (koh, cellophane tape culture, and hairbrush culture ...200414765095
antibacterial and antifungal activity of senecio inaequidens dc. and senecio vulgaris l.the antibacterial and antifungal activities of methanol extract, ethyl acetate, dichloromethane, n-hexane, n-butanol and chloroform fractions of senecio inaequidens dc. and senecio vulgaris l. were assayed. the hexane extract of senecio vulgaris l. (mic 0.031 mg/ml) showed significant activity against trichophyton tonsurans.200415478193
tinea capitis in infants in their first year of life.tinea capitis is the most common type of dermatophytosis in children, but is uncommon in the first year of life.200415491432
molecular typing of trichophyton tonsurans by pcr-rflp of the ribosomal dna nontranscribed spacer region. 200415519147
[experimental studies on the penetration of dermatophytes into the human stratum corneum and the effects of washing].after several types of dermatophytes were applied to the stratum corneum obtained from a healthy human heel, we evaluated the penetration speed of fungal elements into the stratum corneum and the effect of washing its surface. we designed the following two environments, assuming tinea pedis after applying fungal elements to the surface of the stratum corneum. the samples were incubated under conditions simulating the daily life of those who wear socks in a house: (1) 90% humidity for 8 hours, an ...200415550923
dermatophytosis in tulugudu island, ethiopia.the objective of this investigation was to assess the prevalence of dermatophytoses in children in a geographically restricted area in the ethiopian countryside, and to determine the aetiological agents of these infections. demographical and clinical-dermatological data were collected from all children 4-15 years of age on tulugudu island, southern ethiopia. mycological specimens were taken and species identification determined through morphological observations and biochemical tests, complement ...200515712611
[tinea capitis in an adult. case report].a tinea capitis and tinea faciei case in an elderly female patient with diabetes mellitus type 2 is presented. her dermatological illness began on her face and later disseminated to her scalp. she received multiple medications which aggravated her condition. the diagnosis was established by means of direct examination and the isolation of trichophyton tonsurans in culture. treatment with 200 mg daily dose of itraconazol for two months resulted in clinical and mycological cure.200515813685
[survey of trichophyton tonsurans infection in the kyushu, chugoku and shikoku areas of japan].a survey on the infestation of dermatophytosis caused by t. tonsurans was made by inquiry to dermatologists. patients strongly suggestive of having skin lesions due to t. tonsurans were seen in all areas examined, and were mainly schoolboys, including those practicing judo, of high schools and junior high schools. in nagasaki, a mycological examination was done on 21 judo students and their teacher in a high school in which a patient with t. tonsurans infection was mycologically confirmed and tr ...200515864256
[an investigation of trichophyton tonsurans infection in university students participating in sports clubs].the prevalence of the infectious disease trichophyton tonsurans is rapidly increasing in japan, particularly in athletes of these sports such as judo. recognition of the current prevalence of this disease not only in athletes of these sports but also in other athletes is important to prevent its further spread.200515864258
repetitive-sequence-pcr-based dna fingerprinting using the diversilab system for identification of commonly encountered dermatophytes.the performance of repetitive-sequence-based pcr (rep-pcr) using the diversilab system for identification of dermatophytes commonly isolated in a clinical laboratory was assessed by comparing results to those of conventional tests (colony morphology, microscopic examination of slide cultures, and, for suspected trichophyton species, use of additional media). sixty-one cultures were tested in phase 1, the feasibility portion of the study; 64 additional cultures were tested in phase 2, the validat ...200515872233
chicory extracts from cichorium intybus l. as potential antifungals.in this work extracts from roots of the common vegetable cichorium intybus l., highly appreciated for its bitter taste, were studied to investigate their possible biological activity on fungi from a variety of ecological environments: some are parasites on plants (phytopathogens) or of animals and humans (zoophilic and anthropophilic dermatophytes), others live on the soil and only seldom parasitize animals (geophilic dermatophytes). the extracts were ineffective on geophilic species and on test ...200516160773
characterization of the alp1 gene locus of trichophyton tonsurans.trichophyton tonsurans is the primary etiologic agent of fungal infections in the pediatric population. establishing techniques that facilitate strain discrimination offer the opportunity to investigate the relationship between fungal genotype, biochemical phenotype and disease presentation in the host. in the process of expanding efforts to elucidate intra-specific genetic variability in t. tonsurans, we have identified 2 genetic polymorphisms in the alp1 gene: a fragment length polymorphism in ...200516244893
outbreak of tinea corporis gladiatorum, a fungal skin infection due to trichophyton tonsurans, in a french high level judo team.an outbreak of 49 cases of tinea corporis gladiatorum due to trichophyton tonsurans infection occurred in a high level judo team of 131 members in orleans, central france, between october 2004 and april 2005. the team was divided into 5 groups: cadet-junior boys (n=44), cadet-junior girls (n=33), male university students (n= 15), female university students (n=21), and a group called 'pole technique' made up of high level judokas who have finished academic study (n=18). the outbreak involved 86% ...200516280611
[a case of black dot ringworm caused by trichophyton tonsurans in chiba prefecture].a 74-year-old woman visited a clinic in kisarazu, chiba prefecture in december 2002 complaining of itching, scale and alopecia. she had been diagnosed as having tinea capitis by a direct microscopic examination of scales, and been treated with an antifungal cream and steroid lotion since 1999. the bald area spread from frontal to occipital in which multiple black dots and red papules were scattered. abundant endothrix spores were observed in the hair shaft. a mycelial colony was isolated from th ...200516282970
prevalence of dermatophytic fungal infections in children: a recent study in anambra state, nigeria.we conducted a recent survey of dermatological fungal infections amongst children in both urban and rural parts of anambra state in the south-east geographical flank of nigeria during the period january 2003 to december 2003. samples were collected from 1624 children with clinically suggestive lesions and also between the ages of 4 and 16. young children aged 7-11 and 4- 6 years had significantly higher incidences (p < 0.05) than their older colleagues aged 12-16 years among samples proved to be ...200516205973
trichophyton tonsurans infection manifesting as multiple concentric annular erythemas.we report a case of dermatophytosis in a judo wrestler caused by trichophyton tonsurans (t. tonsurans) with clinical features that mimicked the concentric rings of tinea imbricata. tinea imbricata is a unique dermatophytosis caused by trichophyton concentricum (t. concentricum), observed endemically in subtropical to torrid zones and characterized by impressive concentric rings. we found three similar cases of the dermatophytosis in the literature that were reported as tinea pseudoimbricata or t ...200516335872
[epidemic of trichophyton tonsurans tinea capitis in a nursery school in the southern suburbs of paris].in march 2001 a school and family survey was conducted in a nursery school in the southern suburbs of paris, during an epidemic of trichophyton tondsurans tinea.200515988354
antifungal susceptibilities of dermatophytic agents isolated from clinical specimens.the aim of this study was to investigate the susceptibility to four antifungal agents: ketoconazole, terbinafine, itraconazole and fluconazole, of the different species of dermatophyte strains isolated from clinical specimens. a total of 128 specimens were collected from toe nail, foot, inguinal region, trunk, hands and head. the dermatophytes tested included trichophyton rubrum 108 (84.4%), trichophyton mentagrophytes 11 (8.6%), epidermophyton floccosum 5 (3.9%), microsporum canis 2 (1.5%) and ...200516048753
[epidemiological survey of trichophyton tonsurans infection in tohoku district and its clinical problems].to research the current status of trichophyton tonsurans infection in tohoku district, i sent out a questionnaire to the main dermatology clinics in the tohoku district. the results showed this infection was found first in spring, 2001 in miyagi prefecture, and gradually spread from southern to northern districts; the total number of patients is now 162. the number in each prefecture is as follows: yamagata; 88, miyagi; 68, akita; 4, aomori; 2. in iwate and fukushima, however, no cases were repo ...200515864253
prevalence of scalp scaling in prepubertal children.to determine the prevalence and most common etiologies of scalp scaling in infants and prepubertal children and the specificity of head and neck lymphadenopathy for the diagnosis of tinea capitis associated with scalp scaling.200515629960
prevalence of tinea capitis and tinea pedis in barcelona schoolchildren.although dermatophytoses can appear at any age, some types are particularly prevalent in children. there are no prior data on the prevalence of tinea capitis and tinea pedis in barcelona, spain. to identify the prevalence of tinea in school children in the area with the highest immigrant population in this city, a cross-sectional study was performed. a second objective was to identify the etiologic agent to study the possibility of the introduction of foreign dermatophyte species and to evaluate ...200515702042
trichophyton tonsurans associated tinea corporis infection with the development of majocchi's granuloma in a renal transplant patient.trichophyton tonsurans is an uncommon cause of tinea corporis, and an even more uncommon cause of majocchi's granuloma. we report a patient who developed tinea corporis with majocchi's granuloma from t. tonsurans infection. immunocompromised hosts are predisposed to develop cutaneous fungal infections, as was the case with this patient. majocchi's granuloma is a rare complication with immunosuppression, but is significant to consider when a fungal infection is suspected because it may require mo ...200516302565
antidermatophytic and bacterial activity of mimosine.mimosine, a non-protein aromatic amino acid was tested at 100, 50 and 25 microg/ml on some human pathogenic bacteria and fungi. mimosine exhibited total lethality towards trichophyton tonsurans and trichophyton rubrum at 100 microg/ml. among the tested bacteria staphylococcus aureus was inhibited to a larger extent than the other bacteria. the studies revealed mimosine to be potent against fungi rather than bacteria. this study reports the effect of mimosine on dermatophytes for the first time.200516317659
role of chrysosporium keratinophillum in the parasitic evolution of dermatophytes.anti-dermatophytic activity of chrysosporium keratinophillum against species of the genera trichophyton, microsporum and epidermophyton floccosum was tested in vitro. when c. keratinophillum and different species of dermatophytes were inoculated on sabouraud's dextrose agar plates 2 cm apart, no antagonistic effect of c. keratinophillum on the mycelial growth of dermatophytes was observed. however, conidia production was not observed on the hyphae of trichophyton rubrum, trichophyton tonsurans a ...200516262884
[questionnaire investigation of incidence of trichophyton tonsurans infection in dermatology clinics in the kanto area].we conducted a questionnaire investigation to learn the incidence of t. tonsurans infection. subjects of this investigation were 1,060 dermatologists in 1,060 dermatology clinics in the kanto area to whom questionnaires were mailed. we asked each dermatologist whether he/she had experienced t. tonsurans infection cases (including suspected cases) and if so, we further asked; a. time of onset, b. number of cases, c. sexuality of the patient, d. club that the subject had joined (judo club, wrestli ...200515864254
[survey of trichophyton tonsurans infection in the hokuriku and kinki regions of japan].in june 2004, information was gathered on trichophyton tonsurans infections, both past and current, in the hokuriku and kinki regions of central-western honshu island, japan, by questionnaires sent to 185 dermatologists who were members of the local medical mycologist associations hokuriku shinkin kondan-kai and kansai shinkin kondan-kai. of the 111 (59.4%) who returned the completed questionnaire, 32 (28.8%) had seen patients infected with t. tonsurans including suspicious cases. the earliest r ...200515864255
[trichophyton tonsurans infection among judo practitioners who attended the national junior high school judo tournament in japan (2005): incidence and therapeutic response].the spread of trichophyton tonsurans infection among high school students and university undergraduates who practice judo is an emerging problem in japan and other countries. however, the extent of infection among judo practitioners in junior high school in japan is unknown. we conducted an epidemiological study of t. tonsurans infection among students who participated in the national junior high school judo tournament in 2005. of the 1,039 tournament participants invited to undergo screening, 4 ...200617086166
[survey of trichophyton tonsurans infection in japan. molecular epidemiology and factors affecting adequate hairbrush sampling].at the 48th annual meeting of the society for japanese medical mycology, held in october, 2004, we reported our findings from a survey on trichophyton tonsurans infections in the hokuriku and kinki regions of japan. the survey revealed that a few epidemics had occurred across these regions. in this article, we introduce our subsequent studies relating to 1) molecular epidemiology of isolates taken from people in many parts of japan and 2) factors affecting adequate sampling of the scalp with hai ...200616699483
onychomycosis in eastern nepal.onychomycosis, a fungal infection of the nail is responsible for up to 50.0% of all nail diseases. though, dermatophytes are most frequently implicated as the causative agents in onychomycosis, yeast and molds are increasingly recognized as causative pathogens. this study was aimed to know the clinical and mycological pattern of onychomycosis in eastern nepal. eighty-two clinically diagnosed patients of onychomycosis attending the dermatology outpatient department of a tertiary hospital over a p ...200617357634
chemical composition and antidermatophytic properties of volatile fractions of hexanic extract from leaves of cupressus lusitanica mill. from cameroon.the chemical composition of five column fractions of hexanic leaf extract of cupressus lusitanica were analysed by gas chromatography and gas chromatography-mass spectrometry and then tested for their antidermatophytic activities using the agar dilution method. the first fraction (f(1)) has only hydrocabon monoterpenes with alpha-pinene (80.0%) as major component. the main constituents of the second fraction (f(2)) were epi-bicyclosesquiphellandrene (35.3%), epi-zonarene (10.3%), 1s, cis-calamen ...200616169171
incidence of tinea capitis in são paulo, brazil.to determine the incidence of tinea capitis in são paulo, brazil, an investigation was performed in private and public pediatrics service involving 4,500 children from 0 to 15 years old during 5 years (1996-2000). samples were taken from 132 children with suspected fungal infection of the scalp, for direct microscopy and culture. tinea of scalp was mycologically confirmed in 112 patients (85%). males were more affected than females in all age groups. children below 8 years old accounted for more ...200616897586
cytokine secretion profiles of human keratinocytes during trichophyton tonsurans and arthroderma benhamiae infections.dermatophytes cause intractable superficial infections in humans. arthroderma benhamiae, a zoophilic dermatophyte, triggers severe inflammatory responses in humans, while trichophyton tonsurans, an anthropophilic dermatophyte, triggers minimal ones. cytokines and other factors derived from keratinocytes play important roles in inflammatory and immune responses in the skin. the authors performed an in vitro investigation to determine the human keratinocyte cytokine profiles during dermatophyte in ...200616914646
the changing pattern of tinea capitis in jamaica.the species of dermatophyte fungi causing tinea capitis vary from country to country and may also change with time. this study was done to identify the predominant organisms causing tinea capitis in the jamaican population. it was a retrospective study looking at all fungal culture requests to the microbiology department at the university hospital of the west indies during the period january 1, 1998 to december 31, 2002. the results showed a gradual switch from the dominance of microsporum audou ...200616921700
real-time pcr taqman assay for detecting trichophyton tonsurans, a causative agent of tinea capitis, from hairbrushes.tinea capitis caused by trichophyton tonsurans is currently an epidemic in the united states, europe, and japan, and the cultivation of this microorganism is necessary for a definitive diagnosis. we recently developed a real-time pcr taqman assay as a culture-independent method for the rapid detection of t. tonsurans from hairbrushes.200616966179
prevalence of dermatophytosis in junior secondary schoolchildren in ogun state, nigeria.a total of 2772 randomly selected junior secondary school pupils (aged 8-14) from 60 schools were examined for dermatophytic infections by direct microscopy and culture-based laboratory diagnostic methods. of these, 641 (23.21%) had dermatophytosis. out of these, 376 (13.56%) were male while 265 (9.56%) were female. aetiological agents identified with infection were microsporum canis (30.19%), microsporum audouinii (32.92%), trichophyton interdigitale (14.37%), trichophyton soudanense (9.73%) an ...200617022768
canine dermatophytosis caused by an anthropophilic species: molecular and phenotypical characterization of trichophyton tonsurans.microsporum canis is the most common species isolated from canine and feline dermatophytosis in the world. however, this study reports a rare case of canine dermatophytosis caused by the anthropophilic dermatophyte trichophyton tonsurans in the city of fortaleza, ceará, brazil. the fungal characterization was performed by classical mycological examination and by genotypical analysis using the restriction enzymes sau3a, rsai, ddei and ecori. the phenotypical characteristics were compatible with t ...200617030920
[in vitro activities of antifungal drugs against clinical isolates of trichophyton tonsurans].to determine drug susceptibility of trichophyton tonsurans endemic in japan, in vitro mics of antifungal drugs against a total of 10 clinical isolates of t. tonsurans collected from dermatophytosis patients were measured by the agar dilution method and the broth microdilution method. the agar dilution method was not appropriate as the growth of t. tonsurans on the agar medium was too slow to determine drug activity, while the broth microdilution method was thought to be an appropriate method for ...200617086163
[tinea capitis in madagascar: a survey in a primary school in antsirabe].tinea capitis is a common infection among schoolchildren in developing countries. this condition is still under-reported in madagascar. in order to assess the prevalence of tinea capitis in madagascar, we conducted a study in a primary school in antsirabe, a town located in the country's high central territories.200616495846
in vitro sensitivity of dermatophytes to urea.urea is commonly used as a keratolytic substance in the treatment of onychomycoses to improve the penetration of antifungal drugs in the lesion sites. the aim of the present study was to investigate the inhibitory action of urea on samples of dermatophytes in vitro.200616532219
kerion and dermatophytic granuloma. mycological and histopathological findings in 19 children with inflammatory tinea capitis of the scalp.inflammatory tinea capitis or kerion is the result of a hypersensitivity reaction to a dermatophytic infection. majocchi's granuloma, in contrast, usually begins as a suppurative folliculitis and culminates in a granulomatous reaction.200616533218
a nationwide survey of trichophyton tonsurans infection among combat sport club members in japan using a questionnaire form and the hairbrush method.the emerging outbreak of trichophyton tonsurans infection among members of combat sports clubs in japan during the last 4 years has become a serious public health problem. to overcome this outbreak, a survey for t. tonsurans infection in japan may be essential.200616546582
a case of trichophyton tonsurans infection in which incubation time can be estimated. 200616556290
tinea capitis: current concepts in clinical practice.tinea capitis is a common infection, particularly among young children in urban regions. the infection often is seen in a form with mild scaling and little hair loss, a result of the prominence of trichophyton tonsurans (the most frequent cause of tinea capitis in the united states). t. tonsurans does not fluoresce under wood light, unlike the common tinea capitis-causing fungal organisms seen in europe and many other countries, which emit a green fluorescence. however, t. tonsurans, like other ...200616570671
aetiological agents of tinea capitis in tehran (iran).a total of 1568 patients with suspected tinea capitis were examined for causative fungal agents between 1994 and 2001. laboratory examination confirmed tinea capitis in 209 patients. males were affected more frequently (67.5%) than females (32.5%) and in both sexes, those who were 3-11 years old, were more infected. trichophyton violaceum was the most common aetiological agent (37.3%) followed by trichophyton schoenleinii (21.5%), microsporum canis (18.6%), trichophyton verrocosum (14.8%), trich ...200616367822
bioactive extracts from senecio samnitum huet.the antimicrobial and cytotoxic activities of extracts from senecio samnitum huet are reported. extracts from s. samnitum were able to inhibit the in vitro proliferation of four human tumor cell lines. the dichloromethane extract demonstrated effective cytotoxic activity with ic50 of 22.89 microg ml(-1) on the caco-2 cell line and the etoac extract had ic50 value of 11.91 microg ml(-1) against the cor-l23 cell line. the n-hexane extract displayed the best antibacterial activity against gram posi ...200616401558
genotype analysis of the variable internal repeat region in the rrna gene of trichophyton tonsurans isolated from japanese judo practitioners.tinea capitis due to trichophyton tonsurans is currently epidemic among japanese judo practitioners. t. tonsurans has seven genotypes in a variable internal repeat (vir) region of the rrna gene. all 101 isolates obtained from japanese judo practitioners had the identical genotype. this suggests that a specific genotype strain occurs throughout japan.200616428874
an experience from an outbreak of tinea capitis gladiatorum due to trichophyton tonsurans.'tinea corporis gladiatorum' describes a dermatophytosis transmitted mainly from close skin contact among wrestlers. although tinea corporis is well recognized, no data are available for tinea capitis infections in wrestlers. after finding tinea capitis infection in a student wrestler, we aimed to search for possible ringworm infections among wrestlers in a wrestling boarding-school. of the 32 wrestlers, 29, aged 12-18 years, were affected, of whom 22 had scalp involvement. trichophyton tonsuran ...200616487093
majocchi's granuloma in a 3-year-old boy.a 3-year-old boy presented with a 15 x 15-cm papulonodular lesion on his left shin of 8 weeks' duration. the lesion was considered a majocchi's granuloma caused by trichophyton tonsurans. he was treated with griseofulvin and topical clotrimazole and hydrocortisone cream and responded well after 8 weeks of treatment.200616804445
[outbreak of cutaneous dermatophytosis in the judo french programme in orleans: september 2004-june 2005].high contact sports regularly allow transmission of infectious agents, including fungi such as dermatophytes. the occurrence of dermatophytosis outbreaks among wrestlers has been extensively described since the 90s. the emergence of such outbreaks among judokas was described for the first time in december 2004. we report here an outbreak which occurred in a high level judo team and is, to our knowledge, the largest ever published. patients et methods: from october 2004 to june 2005, every judoka ...200616885838
dermatophytoses in monterrey, méxico.in the present report we reviewed a total of 2397 cases of dermatophytosis from superficial cutaneous lesions between the years 1978 and 1990. the cases included were from the department of dermatology at the university hospital located in the city of monterrey, méxico. a total of 726 tinea pedis, 613 tinea unguium, 441 tinea capitis, 395 tinea corporis and 222 tinea cruris cases were observed. the most commonly isolated dermatophyte species was trichophyton rubrum (45%), followed by trichophyto ...200616466445
antifungal effects of aminosulphoxide and disulphide derivatives.2-benzenesulphinyl-(1,4)-naphtoquinone and 14 derivatives were synthesised and were used to evaluate their cytotoxicity against a human myelomonocyte cell line and their antifungal activity against two yeast, i.e. candida albicans and c. tropicalis and against two filamentous fungi such as aspergillus niger and fusarium oxysporum and against one dermatophyte, namely trichophyton tonsurans. the cytotoxicity and antifungal activities were investigated in comparison with amphotericin b as reference ...200616681806
tracking trichophyton tonsurans through a large urban child care center: defining infection prevalence and transmission patterns by molecular strain typing.trichophyton tonsurans is the single most common cause of pediatric dermatophytoses in north america and is observed with increasing frequency in other countries. this investigation was designed to gain insight into the natural course of t. tonsurans infection.200617142520
rapid identification of trichophyton tonsurans by specific pcr based on dna sequences of nuclear ribosomal internal transcribed spacer (its) 1 region.trichophyton tonsurans, a dermatophyte implicated in an international epidemic of tinea capitis, was also found to be responsible for infecting wrestling and judo athletes nationwide in japan since 2001.200616488118
tinea capitis in a paediatric population.tinea capitis is an increasing problem in europe. the pattern of infection is changing with an increase in pathogenic anthropophilic dermatophytes particularly trichophyton tonsurans. we aimed to determine the frequency of tinea capitis in a paediatric population attending dermatology outpatients and examine the clinical spectrum of disease. a retrospective analysis was performed of all laboratory proven tinea capitis cases presenting to the dermatology outpatient department at the children's un ...200617274169
clinico-mycological profile of dermatophytosis in a reference centre for leprosy and dermatological diseases in addis ababa.to assess the clinical and fungal species spectrum of dermatophyte infection in a reference centre in addis ababa, 539 dermatological patients with signs of dermatophytosis were investigated. seventy-one percent were female and 29% male, aged 2-66 years (median 9). four hundred-fifteen (77%) had at least one skin lesion. tinea capitis was diagnosed in 138/155 males (89%) as compared to 214/384 females (40%) (p < 0.05). t. capitis was diagnosed in 69% of the 374 children. fingernails were affecte ...200616482389
asymptomatic dermatophyte scalp carriage in school children in adana, turkey.the aim of this study was to determine the prevalence of asymptomatic dermatophyte scalp carriage and symptomatic tinea capitis in adana province, cukurova region, turkey. for this purpose, a screening study was performed in five schools, between january 2004 and may 2005, covering a total of 5143 children with 2740 (53.3%) boys and 2403 (46.7%) girls, aged 7-14 years (9.6 +/- 2.0). the diagnosis was made using the cotton swab method with inoculation onto sabouraud glucose agar amended with cycl ...200717305777
[epidemiology, etiology and clinical presentation of dermatophytosis in pernambuco, 1995-2005].out of a total of 1,238 cases of dermatophytosis in the city of recife (pernambuco), lesions of the scalp (33.7%) and trichophyton tonsurans (25.5%) predominated between 1995 and 2000, while lesions of the hairless skin (35.5%) and trichophyton rubrum (34%) were the most frequent between 2000 and 2005. a significant reduction in trichophyton mentagrophytes was detected in the second of these periods.200717876478
[tinea capitis - a recurring problem].in the first quarter of the 20th century, tinea capitis represented the most important dermatomycological diagnosis in europe. for its significant social impacts (affecting children, potential permanent disfiguration), developed countries introduced strict anti-epidemic measures which, together with the discovery of griseofulvin in 1960, contributed to nearly complete eradication of the disease in central europe. since 1980s, however, the incidence of tinea capitis has increased to become a seri ...200717929221
[a case of black dot ringworm on the right forearm caused by trichophyton tonsurans].we report a case of black dot ringworm on the right forearm caused by trichophyton (t.) tonsurans. a 16-year-old male high school judo-wrestler visited our clinic on september 30, 2005, complaining of a round erythematosquamous eruption with a distinct margin on the right forearm. black dots were observed in the lesion. koh-parker ink prepared direct microscopy revealed abundant large endotrix arthroconidia in the hair shaft. culture yielded yellowish-brown colonies. the isolate produced numerou ...200717502844
cytobrush-culture method to diagnose tinea capitis.this is a comparative study to isolate the dermatophytes of tinea capitis using the cytobrush and comparing it versus the standard method. a prospective, observational, comparative trial of 178 probable cases of tinea capitis was conducted in two dermatological centers. each patient underwent mycological tests that included direct exam with koh and cultures with either of two methods: scraping the scalp to remove hair and cell debris, and the cytobrush. a total of 135 clinically and mycologicall ...200717520340
prevalence of tinea capitis in southern kuwait.tinea capitis of the scalp, an infection caused by dermatophytes, produces a significant health problem especially among school children. the object of this study was to highlight the prevalence of tinea capitis in southern kuwait. during this retrospective study from 1998 to 2003, 1737 suspected cases were examined, 986 (58.6%) were men and 751 (43.2%) were women of which 371 cases were diagnosed as tinea capitis. of 371 cases of tinea capitis, males comprised 54.2% and females 45.8%. young chi ...200717576327
molecular epidemiology of trichophyton tonsurans isolated in japan using rflp analysis of non-transcribed spacer regions of ribosomal rna genes.trichophyton tonsurans has been reported to be the causative agent of an epidemic of tinea corporis and tinea capitis among japanese judoists and wrestlers. a molecular method using restriction enzyme analysis of pcr-amplified fragments targeting the non-transcribed spacer (nts) region of ribosomal rna genes in fungal nuclei was applied to a total of 232 strains of t. tonsurans isolated in japan. six molecular types, i.e., nts types i, ii, iii, iv, v, and vi, were clearly detected in restriction ...200717642528
epidemiology of dermatophytoses in a rural community in eastern nigeria and review of literature from africa.a total of 4,287 primary school children, comprising 1,740 males and 2,547 females in arochukwu local government area of abia state nigeria were examined for clinical signs of dermatophytoses. about 873 (20.4%), consisting of 505 males and 368 females had lesions consistent with dermatophytoses. the disease was more prevalent in males (29%) than females (14.4%) in a ratio of approximately 2:1 (p < 0.05). the infection rate increased from 16.8% in the 4-6 year age group to a peak of 28.1% in the ...200717657581
epidemiology of tinea capitis in europe: current state and changing patterns.tinea capitis (scalp ringworm) is the most common dermatophyte infection of the scalp affecting mainly children and rarely adults. the epidemiology of tinea capitis varies within different geographical areas throughout the world. it may occur sporadically or epidemically and an increase in its incidence has been noted over the last few decades. the aim of the study is to obtain a general overview of the current state and changing pattern of tinea capitis in europe. according to the literature, t ...200717681048
trichophyton tonsurans infection mimicking tinea imbricata. 200717397364
multilocus genotyping identifies infections by multiple strains of trichophyton tonsurans.acquisition of multiple genetic strains of a single dermatophyte species should not be unexpected in areas of high endemicity, and yet multistrain infections are infrequently reported. this communication details mixed trichophyton tonsurans infections and highlights the need to confirm the presence of multiple strains in a clinical single isolate by use of a multilocus approach.200717442802
five-hour diagnosis of dermatophyte nail infections with specific detection of trichophyton rubrum.a rapid two-step dna extraction method and a multiplex pcr for the detection of dermatophytes in general and trichophyton rubrum specifically were developed and evaluated with dna extracted from pure cultures and from clinically diseased nails. dna from the following dermatophytes was used: epidermophyton floccosum, microsporum audouinii, microsporum canis, microsporum gypseum, microsporum nanum, trichophyton mentagrophytes, trichophyton rubrum, trichophyton schoenleinii, trichophyton soudanense ...200717267633
a study on tinea gladiatorum in young wrestlers and dermatophyte contamination of wrestling mats from sari, iran.to study the prevalence of tinea gladiatorum among young wrestlers and dermatophyte contamination of wrestling mats from sari city, the capital of mazandaran, a northern province of iran.200717138633
its barcodes for trichophyton tonsurans and t. equinum.early molecular biosystematic studies of dermatophytes created considerable confusion about the taxonomic status of the horse-associated trichophyton equinum vis-à-vis the anthropophilic t. tonsurans. though this matter has recently been clarified, routine identification of these species based on the commonly used ribosomal internal transcribed spacer (its) sequence has been impractical. this is because, in the available sequences attributed to the species in genbank, a clear species-level disti ...200717464840
[clinical study of 57 cases of infection with trichophyton tonsurans examined at a dermatology clinic in saga prefecture, japan].this paper is a clinical study of 57 cases of infection with trichophyton tonsurans (t. tonsurans) examined in our clinic between january 2004 and july 2006. the patients were 31 high school students, 19 junior high school students, 2 primary school students, 1 kindergartener, and 4 sports instructors. the male:female ratio was 51:6. most patients were male judo practitioners. patients were clinically categorized as follows: 13 cases of tinea capitis {10 containing black dot ringworms (bdr), 2 s ...200717502842
in vitro activity of citrus bergamia (bergamot) oil against clinical isolates of dermatophytes.recently, bergamot oil was shown to be a potent antifungal agent in vitro against clinically important candida species. in this study, the activities of bergamot natural essence and its furocoumarin-free and distilled extracts on dermatophytes such as trichophyton, microsporum and epidermophyton species were investigated.200717118937
closely related dermatophyte species produce different patterns of secreted proteins.dermatophytes are the most common infectious agents responsible for superficial mycosis in humans and animals. various species in this group of fungi show overlapping characteristics. we investigated the possibility that closely related dermatophyte species with different behaviours secrete distinct proteins when grown in the same culture medium. protein patterns from culture filtrates of several strains of the same species were very similar. in contrast, secreted protein profiles from various s ...200717156126
laboratory-based epidemiological study of superficial fungal infections.dermatophytosis continues to be a worldwide problem, constituting a large bulk of cases attending the dermatology outpatient's department in tropical countries. variable climatic conditions and multiple etiological agents, whose individual prevalence varies with time, prompted an attempt to define the current pattern and etiologic prevalence in our locality, and compare them with earlier studies from different centers. of consenting patients clinically diagnosed to have superficial fungal infect ...200717352722
screening for asymptomatic carriage of trichophyton tonsurans in household contacts of patients with tinea capitis: results of 209 patients from south london.there is currently an epidemic of tinea capitis in urban areas of developed countries caused by trichophyton tonsurans. recurrence or re-infection with dermatophyte is not uncommon after adequate oral treatment. asymptomatic carriers who are household contacts may partly explain this observation by forming a reservoir for infection.200717714125
in vitro activity of penicillium chrysogenum antifungal protein (paf) and its combination with fluconazole against different dermatophytes.strains of five dermatophyte species (microsporum canis, microsporum gypseum, trichophyton mentagrophytes, trichophyton rubrum and trichophyton tonsurans) were selected for testing against penicillium chrysogenum antifungal protein (paf) and its combination with fluconazole (fcz). inhibition of microconidia germination and growth was detected with mics of paf ranging from 1.56 to 200 microg ml(-1) when it was used alone, or at constant concentration (100 microg ml(-1)) in combination with fcz at ...200818574706
[the part of a hospital dermatologist. prevention of trichophyton tonsurans infection].trichophyton tonsurans infection was found first in autumn 2000 in gifu prefecture and spread rapidly in the tokai region. not only direct koh examination but also culture is necessary to diagnose this disease. in order to collect a specimen, i recommend cellophane adhesive tape. during the past 5 years, dermatologists in the tokai area have sent me specimens in an envelope for mycological examination. hyphae were found in all 75 cases in the scales examined. fungal culture revealed 61 cases to ...200818689969
[results of questionnaire survey of trichophyton tonsurans infection in judo clubs registered under the all japan judo federation].it has been seven years since an outbreak of trichophyton tonsurans infection occurred in japan. we have examined the state of t. tonsurans infection by a hairbrush test of athletes and a questionnaire survey of doctors' experience in its treatment, but have not completely grasped the actual state of infection at the sites of judo matches and its treatment or application of preventive measures. subjects and methods: the questionnaire was distributed to the leaders of all judo clubs (10,077 clubs ...200818689970
commonly affected body sites in 92 japanese combat sports participants with trichophyton tonsurans infection.outbreaks of trichophyton tonsurans infection constitute one of the serious problems among combat sports practitioners in japan. to facilitate the diagnosis of individuals at risk, we undertook a study to determine which body sites are most commonly infected. we reviewed medical data, hairbrush culture results and questionnaire information from patients with t. tonsurans infection who were admitted to the dermatology clinic of juntendo university hospital from 2000 to 2004. the study included 92 ...200818793263
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