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susceptibility of sixty-five non-oral clinical isolates of the streptococcus milleri group to seven antimicrobial agents.antimicrobial susceptibilities of sixty-five non-oral streptococcus milleri group clinical isolates to penicillin, gentamicin, lincomycin, ampicillin, chloramphenicol, tetracycline and erythromycin were determined by an agar dilution method. all strains were penicillin-sensitive (mic < or = 0.031 microgram/ml) and the majority (64/65) were susceptible to erythromycin (mic < or = 0.125 microgram/ml). low-level resistance to gentamicin was observed, and the majority of strains possessed an mic of ...19921453983
the endodontic microflora revisited.the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ...19921479923
[liver abscess caused by streptococcus milleri]. 19921493888
restricted distribution of streptococcus milleri carbohydrate type antigens amongst other viridans streptococci.the distribution of oral streptococcus milleri carbohydrate type antigens in other viridans streptococcus species was examined. rantz-randall extracts of cells of the test strains grown in broth containing glucose were allowed to react with typing or grouping antisera for s. milleri serotypes a-k, or lancefield groups a-g and k. of 93 strains comprising more than 12 streptococcal species that included s. mutans and s. sanguis complexes, only 15 s. salivarius strains and one s. mitis strain were ...19921518038
effects of arginine and arginyl-l-arginine on the glucose-mediated ph fall of streptococcus rattus and streptococcus milleri (short communication). 19921521307
streptococcus milleri group: renewed interest in an elusive pathogen.the following review examines the bacteriological characteristics, epidemiology, pathogenicity and antimicrobial susceptibility of the "streptococcus milleri group". "streptococcus milleri group" is a term for a large group of streptococci which includes streptococcus intermedius, streptococcus constellatus and streptococcus anginosus. usually considered commensals, these organisms are often associated with various pyogenic infections including cardiac, abdominal, skin and central nervous system ...19921526231
[pyogenic liver abscess caused by streptococcus milleri].streptococcus milleri is a facultative anaerobic pathogen of the viridans group associated with serious suppurative infections, mainly abscesses. we saw 3 cases of liver abscess due to this organism in 1990 in women aged 43, 59 and 67 years, respectively. the coccus was grown from blood cultures in 2 of the cases and from abscess drainage in the 3rd. the importance of prompt diagnosis of liver abscess by means of ct and ultrasonography is stressed. conservative treatment consists of ct-guided pe ...19921526542
serological and biological characteristics of "streptococcus milleri" isolates from systemic purulent infections.ninety-one streptococcus milleri strains isolated from various systemic purulent lesions of 68 patients were examined by physiological and serological tests. most strains formed a smooth colony (66 strains), did not form spontaneous aggregation of cells in bhi broth culture (79), were non-beta-haemolytic (alpha-35 or non-41), and belonged to biotype ia (49) or ib (34) and to api taxa s. milleri i (41) or ii (38). almost all of the beta-haemolytic strains as well as two-fifths of the non-beta-hae ...19921548688
a numerical taxonomic study of the "streptococcus milleri" group based upon conventional phenotypic tests and pyrolysis mass spectrometry.clinical strains presumptively identified as streptococcus milleri (60), and blind coded collection strains (21) were characterised in conventional tests and pyrolysis mass spectrometry. comparison of the clusters found by these two approaches revealed five clearly distinct centres of variation. three corresponded to the dna homology groups suggested by whiley and hardie (1989) as representing the species s. anginosus, s. intermedius and s. constellatus; a fourth comprised three lancefield group ...19921548689
differentiation of species in human beta-haemolytic group g streptococci using immunoglobulin fc fragment receptor.to assess the ability of human immunoglobulin fc fragment binding activity to differentiate human biotype large colony group g streptococci from the group g "streptococcus milleri group".19921556232
septic streptococcus milleri prepatellar bursitis.the osteoarticular affinity of streptococcus milleri has only recently been recognized. we report a case of septic prepatellar bursitis caused by this pathogen. the recent literature concerning osteoarticular involvement by s. milleri is reviewed.19921582108
etiology and antibiotic susceptibility of bloodstream streptococcus sp.249 episodes of streptococcus bacteraemia in hospitalized patients were evaluated for both clinical and microbiological features. specification of the isolates demonstrated that infective endocarditis was predominantly associated with streptococcus sanguis and streptococcus bovis whereas streptococcus pyogenes and streptococcus milleri were the most common cause of local and/or systemic infections. in vitro-susceptibility tests towards 9 selected antibiotics proved that beta-lactam antibiotics a ...19921611209
[pansinusitis of the face caused by streptococcus milleri with cerebral thrombophlebitis after naso-sphenoid surgery of the pituitary gland].the authors report a patient operated on trans-nasosphenoidally for a pituitary adenoma (cushing's disease) in whom a severe infection occurred with cavernous thrombophlebitis responsible for neurological complications.19921620434
fatal basilar vasculopathy complicating bacterial meningitis.bacterial meningitis complicated by thrombosis, vasculitis, and aneurysm formation affecting both small and distal branches of cerebral vessels has been well described. involvement of major cerebral vessels is rare and has only been documented late in the course of disease.19921636194
streptococcus intermedius, streptococcus constellatus, and streptococcus anginosus (the streptococcus milleri group): association with different body sites and clinical infections.the associations of streptococcus intermedius, s. constellatus, and s. anginosus (the three species of the s. milleri group) with clinical infections and sites of isolation were investigated by using a simple biochemical scheme to identify a collection of 153 clinical isolates. s. intermedius was associated with abscesses of the brain and liver, while both s. anginosus and s. constellatus were isolated from a wider range of sites and infections. s. anginosus strains predominated in both genitour ...19921734062
construction of a model secretion system for oral streptococci.a dna fragment corresponding to the secretory domain from the streptococcus mutans gs-5 gtfb gene, which encodes the putative 38-amino-acid signal peptide of the glucosyltransferase i (gtf-i) enzyme product, has been constructed. this fragment was fused with the alpha-amylase structural gene from alkalophilic bacillus sp. strain 707. this hybrid gene as well as the intact amylase gene were introduced into an escherichia coli-streptococcus shuttle vector consisting of three components: the e. col ...19937689539
an in-vitro study of the comparative viability on different swab types of simulated specimens of bacteria commonly present in oro-facial infections.an in-vitro study was undertaken to investigate the survival on bacteriological swabs of three potentially pathogenic organisms found in oro-facial infections, by using simulated clinical specimens incubated in the presence or absence of amies transport medium. standard inocula of pure cultures of streptococcus milleri, prevotella melaninogenica and peptostreptococcus anaerobius were soaked on to swabs and plated out on to solid media at various intervals up to 3 days, enabling an estimate of th ...19938099810
streptococcus milleri liver abscess presenting as fulminant pneumonia.a case of a young man who developed a streptococcus milleri liver abscess secondary to an occult colonic foreign body, which presented as fulminant community acquired pneumonia, is reported. the isolation of streptococcus milleri, a normal member of the gastrointestinal flora, from an abscess, blood or sputum should prompt a search for pathology, foreign bodies or silent perforation in the gastrointestinal tract.19938311805
[streptococcus milleri liver abscess during typhoid fever]. 19938322421
recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms.the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ...19938324113
bacteriologic findings in peritonsillar abscesses in young adults.aspirated pus samples from 124 patients with peritonsillar abscess were cultured quantitatively for aerobes and anaerobes. a total of 98% of the samples yielded bacteria. of the 550 isolates obtained (mean, 4.4 per patient), 143 were aerobes (representing 16 species or groups) and 407 were anaerobes (representing 40 species or groups). aerobes were isolated from 86% of patients-alone in 20 cases and together with anaerobes in 87. the most common aerobic isolates were streptococcus pyogenes (isol ...19938324134
acute sternal osteomyelitis caused by streptococcus milleri. 19938370937
production, characterization, and application of monoclonal antibodies which distinguish three glucosyltransferases from streptococcus mutans.thirty-three murine monoclonal antibodies (mabs) against the three glucosyltransferases (gtfs) (gtf-i, -si, and -s) from streptococcus mutans were obtained by the fusion of murine myeloma cells (p3x63-ag8-u1) with spleen cells of balb/c mice immunized with pure gtf-s or partially purified gtf-i from serotype c s. mutans ps14. the immunoreactivities of these mabs were tested by enzyme-linked immunosorbent assay and western blotting (immunoblotting) with various gtf preparations. gtf-i and gtf-si ...19938418055
phagocytosis of bacterial strains isolated from acute dentoalveolar abscess.the phagocytosis by human polymorphonuclear leucocytes of 37 bacterial strains identified as streptococcus milleri (10 strains), strictly anaerobic gram-positive cocci (10) prevotella intermedia (6), pr. oralis (5) and fusobacterium nucleatum (6) was investigated in vitro. the ingestion of s. milleri and strictly anaerobic gram-positive cocci was significantly greater (p < 0.001) than that of strains of prevotella spp. and f. nucleatum. the degree of uptake of capsulate and non-capsulate strains ...19938429541
contemporary management of deep infections of the neck.odontogenic infections rarely lead to involvement of the lateral and retropharyngeal spaces. when this does occur, the microbiology of the infection is similar to the typical odontogenic infection, ie, streptococcus and oral anaerobes including peptostreptococcus, bacteroides, and fusobacterium. there is an increased incidence of fusobacterium seen in the more severe infections, as well as a higher incidence of streptococcus milleri. many patients who have deep cervical infections also have some ...19938445462
role of group c beta-hemolytic streptococci in pharyngitis: epidemiologic study of clinical features associated with isolation of group c streptococci.all lancefield group c beta-hemolytic streptococci isolated over 12 months from college students with clinical pharyngitis and age-matched healthy controls were identified. clinical features of upper respiratory tract infection and pyogenic pharyngitis as well as colony counts were tabulated for each patient according to throat culture results. of 1,480 patients, lancefield group c streptococcus equisimilis was isolated from 45 (3%) patients and streptococcus anginosus ("streptococcus milleri") ...19938463391
[febrile cervicalgia caused by streptococcus milleri external pachymeningitis]. 19938469658
isolation of gram-positive rods that resemble but are clearly distinct from actinomyces pyogenes from mixed wound infections.beginning in 1990, gram-positive rods resembling actinomyces pyogenes were found with increasing frequency in mixed cultures from various infectious processes, most of them from patients with otitis, empyema, pilonidal cysts, perianal abscesses, and decubitus ulcers. ribotyping and hybridization showed that these gram-positive rods could be divided into five groups not related to known actinomyces species. biochemical markers for reliable differentiation into these groups, however, could not be ...19938501213
deoxyribonucleic acid relatedness and phenotypic characteristics of oral streptococcus milleri strains.strains of oral streptococcus milleri group were compared with the type strains of streptococcus anginosus, s. intermedius, and s. constellatus by dna-dna hybridization at 60 degrees c. of the 29 strains tested, twelve, twelve, and two strains were closely related to s. anginosus atcc 33397t, s. intermedius atcc 27335t, and s. constellatus atcc 27823t, respectively. generally, the strains classified in the s. anginosus group in dna homology were non-beta-haemolytic and belonged to api s. milleri ...19938502174
microbiology of cerebral abscess: a four-year study in malaysia.a prospective study was carried out to determine the aetiology of cerebral abscess in relation to the primary source of infections. seventy-five patients with cerebral abscess were included in the study in the period january 1985 to december 1988. more than half of the patients studied had single lesions and the overall most common sites were in the frontal and parietal regions. chronic suppurative otitis media, cyanotic congenital heart diseases and meningitis were among the important predispos ...19938505776
[bacteriological and clinical studies in 23 cases of thoracic empyema--the role of oral streptococci and anaerobes].the bacteriology of empyema fluid and the clinical background of 23 cases from july 1987 through july 1992 were studied. nineteen cases were male and 4 female, with a mean age of 59.6 years (range; 33 to 84 y.o.). there were 15 cases of community-acquired infection and 8 of nosocomial infection. acute pneumonia and/or lung abscess developed into empyema in 19 cases. sixteen cases had associated predisposing conditions, such as diabetes, chronic bronchitis, disorders with dysphagia, and excess al ...19938515622
[evaluation of phenotype characteristics of streptococci in the identification of the streptococcus anginosus (streptococcus milleri) group].microorganisms referred to as streptococcus anginosus (streptococcus milleri) group which consist of s. anginosus, s. intermedius and s. constellatus is very difficult to identify to the species level, because of their diversity of biochemical, hemolytic and serological characteristics and because of confusion surrounding their taxonomy. identification procedures on three isolates from primary sterile sites of three different patients, using api 20a system are described. streptococcus intermediu ...19938170272
immunochemical characterization of the carbohydrate antigens of serotype c/lancefield group c "streptococcus milleri".carbohydrate antigens of the serotype c/lancefield group c "streptococcus milleri" were extracted by autoclaving whole cells of the type c reference strain k51y. the type c and group c antigen molecules were separated and partially purified by a deae-sephadex a-25 column chromatography followed by sephadex g-100 gel filtration. the purified type c antigen and group c antigen were homogeneous in the double diffusion and in the immunoelectrophoresis. the type c antigen was composed principally of ...19938233569
albumin-binding proteins on the surface of the streptococcus milleri group and characterization of the albumin receptor of streptococcus intermedius c5.members of the streptococcus milleri group (smg) that react with lancefield group c antisera were shown to bind large amounts of albumin although there was no direct relation between these two properties as polyclonal antisera to lancefield group c antigen did not prevent the binding of albumin. there was a specificity for albumin binding, with albumin from man, monkeys, cat, dog and mouse being bound to a greater degree than albumin from cow, horse, goat or rabbit. gold-labelled albumin was sho ...19938254315
haemagglutinating activities of oral strains of streptococcus milleri group.of a total 148 strains of streptococcus milleri group, 66 agglutinated sheep erythrocytes. the haemagglutinating strains were confined to serotypes g, h, i, j or were untypeable, and had no lancefield group antigens (a to g and k), all of which have been shown to belong to streptococcus intermedius. cell surface hydrophobicity did not significantly differ even between the agglutinating and non-agglutinating s. intermedius strains. the haemagglutinating activity of strain 0813-1 (serotype i) was ...19938255256
streptococcus milleri as a cause of antecubital abscess and bacteremia in intravenous drug abusers.s milleri should be added to the list of organisms producing wound infections in parenteral drug addicts. recovery of "viridans streptococci" from an antecubital aspirate should prompt the clinician to request speciation, examine closely for abscess formation, and anticipate prolonged antibiotic therapy.19948284729
a mechanism of pathogenicity of "streptococcus milleri group" in pulmonary infection: synergy with an anaerobe.the relationship between streptococcus constellatus, one of the species of the "streptococcus milleri group", and prevotella intermedia was studied in a model of pneumonia in mice and in vitro to elucidate mechanisms of pathogenicity in "s. milleri group"-associated pulmonary infection. acute pneumonia with or without empyema and lung abscess in mice with mixed infection resulted in 60% mortality rate, but there was only 10% mortality and mild pneumonia in each separate infection. bacterial clea ...19948107060
[streptococcus milleri, causative agent of severe purulent infections]. 19948121507
[pneumonia caused by the 'streptococcus milleri group']. 19948152034
intervertebral infection caused by streptococcus milleri. a case report.intervertebral infection caused by streptococcus milleri is described in a 45-year-old man. initially, a tuberculous infection was suspected; this diagnosis resulted in a considerable delay in correct diagnosis and treatment. the patient recovered well with combined medical-surgical treatment. streptococcus milleri, a common inhabitant of the gastrointestinal tract, comprises a variety of strains implicated in deep-seated purulent infections. although reports of this are rare, s. milleri can be ...19948168298
bacteremia involving the "streptococcus milleri" group: analysis of 19 cases.during a 3-year study period, 19 patients at the university hospital of maastricht developed bloodstream infections with species of the "streptococcus milleri" group, for an incidence of 0.33 per 1,000 admissions. the patients' median age was 48 years; the male-to-female ratio was 2.8. eleven patients (57.9%) had underlying diseases, among which malignancy was predominant. local trauma to the mucosal barrier was an important risk factor. an associated site of infection was found most frequently ...19947803636
bacteriologic characteristics and antimicrobial susceptibility of 70 clinically significant isolates of streptococcus milleri group.the phenotypical characteristics of 70 clinical isolates of streptococcus milleri group were analyzed. association of the three species with particular sites of isolation could not be demonstrated in all cases, but s. anginosus strains predominated in abdominal area, while s. intermedius was isolated mainly in hepatic abscesses. penicillin g and other beta-lactams showed good in vitro activity against these streptococci, whereas > 10% exhibited resistance to erythromycin and clindamycin. resista ...19947805358
lancefield group c streptococcus milleri group strains aggregate human platelets.lancefield group c streptococcus milleri group (smg) strains are the only smg types that are able to aggregate human platelets. complete aggregation occurred within 10 min of mixing bacterial cells and platelets together in the ratio 8:1. substances which (i) chelated cations; (ii) inhibited the cycloxygenase pathway in platelets; (iii) reduced the availability of adp and disrupted platelet membrane stability; (iv) reduced bacterial aggregation of platelets. the platelet-interacting substance on ...19947830532
presence of the listeria tetracycline resistance gene tet(s) in enterococcus faecalis.two hundred thirty-eight tetracycline- and minocycline-resistant clinical isolates of enterococcus and streptococcus spp. were investigated by dot blot hybridization for the presence of nucleotide sequences related to tet(s) (first detected in listeria monocytogenes bm4210), tet(k), tet(l), tet(m), tet(o), tet(p), and tet(q) genes. the tet(s) determinant was found in 22 strains of enterococcus faecalis, associated with tet(m) in 9 of these isolates and further associated with tet(l) in 3 of thes ...19947840565
rapid differentiation of streptococcus milleri from other beta-haemolytic group a, c, and g streptococci by simple screening tests.eighty five beta-haemolytic lancefield group a(23), c(22), and g(40) streptococci were tested for sulphonamide sensitivity, ability to degrade beta-d glucuronide (groups c and g) and bacitracin sensitivity (group a). identification of isolates was initially confirmed by the api 20 strep system. zones of beta-haemolysis were too variable for correct streptococcus milleri identification in 10% of cases. however, only group c and g s. milleri were both sulphonamide-resistant and beta-d glucuronide- ...19947841829
[parenchymatous abscess in streptococcus anginosus (streptococcus milleri) septicemia. value of their systematic search, apropos of 4 cases].streptococcus anginosus, the term suggested to cover a set of streptococci previously known under various names (milleri, mg, anginosus, intermedius, constellatus), is characterized by a propensity to create parenchymatous abscesses, essentially cerebral or hepatic, particularly within the terms of septicemia. these abscesses are sometimes difficult to detect due to a difficult or non-existent symptomatology. the authors report on four cases illustrating the necessity to search for them systemat ...19947846385
two cases of lateral sinus thrombosis presenting with extracranial head and neck abscesses.two cases of lateral sinus thrombosis in fit adults with no previous otological history are presented. one case occurred in association with bezold's abscess and followed mastoiditis which was masked by previous antibiotic treatment. the other case occurred in association with an occipitoparietal scalp abscess and there was no obvious preceding middle ear infection. the causative bacteria were streptococcus milleri and a variant streptococcus intermedius. lateral sinus thrombosis is discussed.19947964144
[septicemia due to streptococcus milleri with pulmonary complications].streptococcus milleri can occasionally cause serious septicemia, that is often complicated by abscesses, particularly pulmonary.19947994335
agglutination of "streptococcus milleri" by lectins.the agglutination of 218 clinical isolates and three atcc type strains of "streptococcus milleri" was tested with 25 different lectins from plants and fungi. an agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. after trypsinisation of the bacteria, 109 strains yielded a positive reaction and after boiling the bacterial cells at ph2, 218 isolates were agglutinated. as an overall result of our experiments with untreated, trypsinised and bo ...19948006940
the clinical importance of gram-positive anaerobic cocci isolated at st bartholomew's hospital, london, in 1987.the clinical importance of the gram-positive anaerobic cocci (gpac) isolated in 1987 at st bartholomew's hospital, london, is assessed. of about 800 anaerobic isolates, 209 (27%) were gpac, of which 67 (32%) were from abscesses and 22 (11%) were in pure growth. four species comprised 77% of the 168 isolates available for study: peptostreptococcus magnus (55 isolates, 33%), p. micros (23, 14%), p. asaccharolyticus (24, 14%) p. asaccharolyticus (24, 14%) and p. anaerobius (27, 16%). different spec ...19948006943
bactericidal action of tachyplesin i against oral streptococci.tachyplesin i, a polycationic antimicrobial peptide isolated from hemocytes of horseshoe crabs, kills bacteria by disrupting the membrane potential of the cytoplasmic membrane. the present study shows that, among 36 oral streptococcal strains, 12 of 21 streptococcus sanguis, 3 streptococcus mutans, 9 streptococcus salivarius and 3 streptococcus milleri strains were susceptible to tachyplesin i, whereas 9 s. sanguis strains were resistant. interestingly, these resistant strains include the clinic ...19948008433
[necrotizing soft tissue infection of the extremities].the term "necrotizing soft tissue infections" describes a group of limb and life-threatening infections. depending on the tissue level, microbiology and clinical course the necrotizing soft-tissue infections are classified in primary located infections to the subcutaneous level and fascia--like hemolytic streptococcus gangrene, necrotizing fasciitis, gram-negative synergistic necrotizing cellulitis, clostridium-cellulitis, anaerobic non-clostridium-cellulitis and in primary located infections to ...19948034528
fulminant septicaemia due to streptococcus milleri infection in a previously healthy adult.a 59-year-old previously healthy female developed purulent pharyngitis leading rapidly to neck cellulitis, septicaemic shock and fatal cardiac arrest despite appropriate antibiotic therapy. streptococcus milleri was isolated from a submandibular abscess, cellulitic neck tissue and blood cultures. fulminant septicaemia due to this group of organisms is relatively uncommon and usually occurs in the immunocompromised or otherwise chronically unwell.19948050438
the effect of indigenous bacterial populations on buccal epithelial cells on subsequent microbial adhesion in vitro.despite the numerous investigations on the adhesion of microorganisms to buccal epithelial cells, it is difficult to correlate different results, as disparate adherence values have been reported for a given organism. as one reason for this disparity may be the indigenous or natural bacterial populations on human buccal epithelial cells, the effect of the latter on subsequent microbial adherence in vitro was examined. there was a highly significant correlation between the degree of natural bacter ...19947478764
streptococcus milleri pulmonary disease: a review and clinical description of 25 patients.streptococcus milleri is increasingly being recognised as an important pulmonary pathogen which may lead to the development of empyema or lung abscess. although several small series have been reported, the clinical and laboratory features have yet to be fully characterised.19957491559
characterization of glucosyltransferaseb, gtfc, and gtfd in solution and on the surface of hydroxyapatite.glucosyltransferaseb, gtfc, and gtfd were purified by hydroxyapatite column chromatography, followed by ultrafiltration from the culture supernatant fluids of three streptococcus milleri constructs (gift from dr. h.k. kuramitsu) which harbored individual gtf genes of streptococcus mutans gs5. gtfb, gtfc, and gtfd were enzymatically active both in solution and in an experimental pellicle (ha-cws-gtf) formed by adsorbing gtf onto the surface of clarified human whole saliva (cws)-coated hydroxyapat ...19957499593
time-dependent efficacy of bacterial filters and infection risk in long-term epidural catheterization.epidural infection represents a serious albeit infrequent complication of long-term epidural catheterization. the catheter hub is regarded as the main point of entry for microorganisms among the three possible routes (hematogenous, insertion site, hub) of microbial colonization of the inserted catheter. the current study was aimed at evaluating whether frequent changing of antimicrobial filters carries an increased risk of catheter hub contamination and the time-dependent efficacy of commonly us ...19957533485
"streptococcus milleri" strains displaying a gliding type of motility.isolates belonging to the "streptococcus milleri" species group that appear to exhibit a gliding type of motility, which is expressed as spreading growth on certain types of agar media, are described. these strains resembled a biotype of "s. milleri" that is usually isolated from genitourinary sources and is notable for its ability to ferment a wide array of carbohydrates. this biotype, which is currently included in the species streptococcus anginosus, has been implicated in cases of neonatal i ...19957537057
heterogeneity among 16s-23s rrna intergenic spacers of species within the 'streptococcus milleri group'.the 16s-23s rrna intergenic spacer has been suggested as a suitable region of the bacterial genome from which to derive useful taxonomic information, particularly with regard to identification at the species level. to investigate this approach as an aid to the identification of the three species comprising the 'streptococcus milleri group' (smg), the spacers of isolates of streptococcus intermedius, streptococcus anginosus and streptococcus constellatus were amplified by pcr and length polymorph ...19957545512
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...19957548575
bilateral panuveitis in a patient with colonic adenocarcinoma and streptococcus milleri liver abscesses. 19957556752
streptococcus anginosus, streptococcus constellatus and streptococcus intermedius. clinical relevance, hemolytic and serologic characteristics.a collection of 518 "streptococcus milleri" isolates recovered from clinical specimens was identified to the species level according to recently established criteria. streptococcus anginosus was the most frequently isolated species (59.3%), followed by s constellatus (30.3%) and s intermedius (10.4%). one third (34%) of all isolates were beta-hemolytic. the majority of s anginosus isolates were nonhemolytic, carried lancefield group f, or were nongroupable. most of the s constellatus isolates we ...19957572815
the high vaginal swab in general practice: clinical correlates of possible pathogens.clinical features, diagnosis and treatment of 286 women whose high vaginal swabs (hvs) submitted by their general practitioners showed pure, heavy growth of staphylococcus aureus, beta haemolytic streptococci groups a, c or g, streptococcus milleri, streptococcus pneumoniae or haemophilus influenzae were analysed. women with group a, c and g streptococci frequently had clinical vulvovaginitis and although the numbers were too small for statistical confirmation, s. pneumoniae and h. influenzae ap ...19957589937
[eight cases of infection caused by the streptococcus milleri group--significance of serum antibody titer and a comparative investigation of the backgrounds and factors of infections caused by streptococcus milleri and streptococcus pneumoniae].eight cases of infection caused by streptococcus milleri were studied clinically, and a comparative study was made of the backgrounds and factors influencing the development of infectious diseases caused by streptococcus milleri and streptococcus pneumoniae. the patients (six males and two females) with infectious diseases caused by streptococcus milleri ranged in age from 21 to 81 years old with an average age of 62.0 years. the infectious diseases encountered among the patients were pyothorax ...19957594780
species identification of members of the streptococcus milleri group isolated from the vagina by id 32 strep system and differential phenotypic characteristics.the importance of bacterial vaginosis as a risk factor in obstetric and gynecological infections has recently been recognized. the bacterial vaginosis group of organisms includes members of the streptococcus milleri group, the identification of which has caused much confusion. we prospectively surveyed the rates of carriage of s. milleri group organisms in 397 high vaginal swabs received in our laboratory. for the identification of 99 clinical isolates and 23 control strains, we compared the res ...19957650193
potential pathogenic properties of members of the "streptococcus milleri" group in relation to the production of endocarditis and abscesses.the "streptococcus milleri" (smg) group have been shown to possess factors in vitro that may be involved in pathogenesis. all smg strains are able to bind fibronectin via a cell-surface protein; the binding ranged from 12 to 198 mol/cell. strains also bound to platelet-fibrin or fibrin clots and fibrinogen, giving maximum adhesion values of 16.5%, 21.8% and 151 mol/cell respectively. members of the species s. constellatus produced thrombin-like activity. lancefield group c smg aggregated rat pla ...19957473673
the effects of x-ray contrast media on bacterial growth.six widely used x-ray contrast media (xrc) were tested against nine commonly isolated organisms to determine the practicality of using xrc to outline body spaces prior to obtaining specimens for culture and to assess the feasibility of using xrc in divided doses to reduce cost. preparations of escherichia coli, klebsiella pneumoniae, enterococcus faecalis, staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosa, candida albicans, streptococcus pyogenes and streptococcus milleri ...19957695517
suppurative infections in children caused by non-beta-hemolytic members of the streptococcus milleri group. 19957716003
pathogenicity of the streptococcus milleri group in pulmonary infections--effect on phagocytic killing by human polymorphonuclear neutrophils.the pathogenicity of streptococcus constellatus in pulmonary infections was investigated in mice in correlation with the phagocytic killing of the microorganisms by human polymorphonuclear neutrophils (pmns). the histological inflammation score of the lungs in mice inoculated intratracheally with 10(8) cfu/mouse of the virulent strain s. constellatus rzyk001 obtained from bronchoalveolar fluid of a patient with pneumonia, was significantly higher than in mice inoculated with the avirulent strain ...19957745309
effects of different acidulants on growth of 'streptococcus milleri group' strains isolated from various sites of the human body.growth of human clinical isolates of streptococcus constellatus, strep. intermedius and strep. anginosus in hcl-, acetate and lactate acidified media was investigated. under aerobic conditions, strep. constellatus and strep. anginosus were significantly more tolerant to all the acidulants than was strep. intermedius. under anaerobic conditions, strep. anginosus and strep. intermedius were significantly more tolerant to acetic acid (ph < 4.5) than strep. constellatus.19957766224
degradative enzymes of oral streptococci.members of the streptococcus sanguis group (ssg) and streptococcus milleri group (smg) were screened for their ability to produce glycosidase, arylamidase (peptidase), protease, dextranase and glycosyltransferase activities. species within each group produced unique patterns of activity. the most commonly produced glycosidases were beta-d-glucosidase, beta-d-galactosidase, n-acetyl-beta-d-glucosaminidase and n-acetyl-beta-d-galactosaminidase and the least commonly produced glycosidase activity w ...19957786231
[fulminant pneumonia caused by streptococcus milleri: rare or not diagnosed?].streptococcus milleri is increasingly isolated in laboratory samples and is worthy of consideration as a differential diagnosis in pyogenic infections, particularly in adults with underlying infections. we describe a well-documented case of pleuropulmonary infection in a young man with no known risk factors. we analyze the diversity of microbiological features of these bacteria in culture, as well as their clinical importance as pathogens.19957788089
neonatal infection with streptococcus milleri.streptococcus milleri is a known commensal of the female genitourinary tract, but its pathogenicity in neonates has been reported in only a few cases. during a period of one year in an obstetrical unit, streptococcus milleri was isolated from nine neonates and from one foetus after a spontaneous abortion. in seven of the nine newborns, neonatal infection was assessed and streptococcus milleri was the lone pathogen involved, associated with positive blood or vaginal cultures in four mothers. beca ...19958536729
intracranial complications of sinusitis: the need for aggressive management.sinus-induced intracranial sepsis can represent a genuine medical and surgical emergency. we review 12 cases presenting to our hospitals over a five-year period. nine were male and three were female with an age range of 16 to 74 years (mean 35.5 years). four patients had their sinusitis diagnosed prior to admission and eight did not. nine patients had bilateral sinus disease, the most common sinus involved was the frontal followed by the ethmoid, maxillary and sphenoid. neurosurgical drainage wa ...19958551120
fibronectin binding by streptococcus milleri group strains and partial characterisation of the fibronectin receptor of streptococcus anginosus f4.the streptococcus milleri group were shown to bind fibronectin (fn) to their cell-surface and this binding increased the adhesion of cells to hydroxyapatite. the binding of fn to streptococcus anginosus f4 was studied in more detail. fn binding to bacterial cells increased the association of the bacteria with the polymorphonuclear leukocytes obtained from the peritoneal cavity of rats but did not increase killing of the bacteria. the cell-surface receptor was a protein of m(r) 14,000 which was r ...19958559042
viridans streptococci isolated from the bloodstream. relevance of species identification.over a 4-year-period, 104 isolates belonging to the viridans streptococci were recovered from the blood and identified to the species according to beighton and co-workers. streptococcus oralis was the species most frequently recovered from patients in the hematology unit [29 of 39 (74%)]. streptococcus mitis ranked second [seven of 39 (18%)]. both species were associated with oromucositis. isolates presently identified as s. oralis are mainly those previously identified as s. mitis or streptococ ...19958565415
identification of "streptococcus milleri" group isolates to the species level with a commercially available rapid test system.clinical isolates of the "streptococcus milleri" species group were examined by conventional methods and a rapid, commercially available method for the identification of these strains to the species level. the levels of agreement between the identifications obtained with the commercially available system (fluo-card milleri; key scientific, round rock, tex.) and conventional methods were 98% for 50 streptococcus anginosus strains, 97% for 31 streptococcus constellatus strains, and 88% for 17 isol ...19958567909
[balanitis and infectious agents. a prospective study of 100 cases].the aim of this study was: 1) to evaluate the rate of micro-organism isolation in 100 patients consulting for balanitis at the centre of sexually transmitted diseases at the st. louis hospital in paris in comparison with that of micro-organisms isolated in 60 men without balanitis; 2) to search for a possible correlation between the clinical aspect of the disease and the nature of the infectious agent identified.19958745679
the streptococcus milleri group as a cause of pulmonary infections.streptococci that colonize the mouth and upper respiratory tract tend to be considered harmless commensals. in 45 cases of acute pneumonia and/or pulmonary abscess and 25 cases of thoracic empyema, the predominant species recovered were anaerobic bacteria and the streptococcus milleri group, which encompasses the oral species streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. the isolation of most s. milleri organisms along with oral anaerobes indicated synergy b ...19958749672
microbiological features and pathogenesis of peritonsillar abscesses.samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ...19958605900
studies into the microbial spectrum of apical periodontitis.this study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. a total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. bacteria were identified by applying the following techniques: culturing on various media, gram-staining and using commercially available biochemical test strips. in addition, prevotella intermedia and porphyromonas endodontalis were d ...19958626206
nonpuerperal breast infection.we undertook a microbiological study of purulent specimens from women with symptomatic breast abscesses.199518476022
isolated apical intracavitary left ventricular abscess in a normal heart: a rare complication of streptococcus milleri endocarditis.a 31-year-old patient without past history of cardiac disease presented with an atypical form of streptococcus milleri endocarditis. the disease progressed in an unusually aggressive manner, with abscess formation in the apex of the left ventricular cavity. the cardiac valves functioned normally and were not affected by the disease.19968634849
degradation and utilisation of chondroitin sulphate by streptococcus intermedius.streptococcus intermedius, part of the 'streptococcus milleri group', has the ability to produce glycosaminoglycan depolymerising enzymes (hyaluronidase and chrondroitin sulphate depolymerase) which is unique amongst the viridans streptococci and may contribute to their virulence in brain and liver abscesses. the growth of s. intermedius strain uns 35 was studied in basal medium supplemented with chondroitin sulphate a (cs-a, sulphated at position 4 of the n-acetylgalactosamine moiety) or chondr ...19968636952
cauda equina syndrome secondary to lumbar spondylodiscitis caused by streptococcus milleri.a 79-year-old afro-caribbean woman presented with a 5-month history of low back pain and a 2-month history of weakness of the lower limbs associated with cauda equina syndrome. a plain x-ray of the lumbar spine showed a collapse of the l4 body consistent with osteomyelitis. an mri scan of the lumbar spine showed narrowing of the l3/4 disc space, destruction of the adjacent vertebral bodies and an epidural abscess. she was admitted to hospital and treated with a high dose of i.v. antibiotics foll ...19968724196
prospective study of streptococcus milleri bacteremia.in a prospective study of bacteremia caused by organisms of the streptococcus milleri group a total of 32 adult patients were observed over a seven-year period. these patients accounted for 1.6% of all patients diagnosed as having significant bacteremia and 17% of all cases of streptococcal (nonpneumococcal) bacteremia diagnosed during the study period. only five patients had polymicrobic bacteremia. in 31 cases, a presumed origin of infection was identified, generally oral or gastrointestinal d ...19968740852
[saprophytic pleuritis with candida parapsilosis].a 73-year-old man was admitted to our hospital with pneumonia in the right s6 induced by streptococcus milleri and with left pleural thickening. he had histories of diabetes mellitus for 30 years and pulmonary tuberculosis 35 years ago. the pneumonia resolved completely after administration of ceftazidime and clindamycin for 10 days, but the pleural thickening remained and computed tomography revealed that it was an encapsulated effusion without calcification. an aspirate was turbid yellow with ...19968778477
rapid species identification of "streptococcus milleri" strains by line blot hybridization: identification of a distinct 16s rrna population closely related to streptococcus constellatus.a collection of 399 "streptococcus milleri" strains were identified to the species level by the use of a line blot assay. their pcr-amplified partial 16s rrna gene sequences were hybridized with species-specific 5'-biotinylated oligonucleotide probes homologous to the bp 213 to 231 regions of the 16s rrna gene sequences of the type strains streptococcus anginosus atcc 33397, streptococcus constellatus atcc 27823, and streptococcus intermedius atcc 27335. the hybridization results were compared w ...19968784576
seven-year review of bacteremia caused by streptococcus milleri and other viridans streptococci.the streptococcus milleri group is associated with a spectrum of serious suppurative infections that have not been well defined. the purposes of this study were to ascertain the clinical significance of streptococcus milleri bacteremia and to determine the epidemiological, clinical, and microbiological features of these infections compared to those caused by other viridans streptococci. all cases of streptococcal bacteremia observed in a spanish hospital in the period from january 1988 to decemb ...19968793393
empyema necessitans due to streptococcus milleri. 19968816169
species belonging to the "streptococcus milleri" group: antimicrobial susceptibility and comparative prevalence in significant clinical specimens.the association between the three species belonging to the "streptococcus milleri" group and different sites of isolation was examined for 73 successive strains recovered from clinically significant, purulent infections. susceptibility testing was performed on 64 of these strains. the present study supports the association of particular species with different clinical sources. susceptibility data suggest that emerging penicillin resistance among streptococcus anginosus and streptococcus intermed ...19968818904
lung abscess in patients with aids.we identified 31 patients with human immunodeficiency virus (hiv) infection and lung abscess. all patients had advanced hiv disease, and the mean cd4 cell count was 17/mm3 (range, 2-50/mm3). twenty-two patients (71%) had previous opportunistic infections, and 24 (77%) had previous pulmonary infections. symptoms at the time of presentation included fever (90% of patients), cough (87%), dyspnea (35%), pleuritic chest pain (26%), and hemoptysis (10%). the microbiological etiology was established fo ...19968824970
interactions between eikenella corrodens and 'streptococcus milleri-group' organisms: possible mechanisms of pathogenicity in mixed infections.interactions between the 'streptococcus milleri-group' organisms (smg; s. intermedius, s. constellatus and s. anginosus) and eikenella corrodens were investigated. coaggregation reactions occurred frequently between s. anginosus (83% of strain combinations) or s. constellatus (87%) and e. corrodens isolates, but were infrequent between s. intermedius and e. corrodens (28%). no enhancement of enzyme activities against lipid, phosphate, peptide and saccharide substrates tested were detected with c ...19968836435
hepatic abscess due to eikenella corrodens and streptococcus milleri: implications for antibiotic therapy.we report a case of intrahepatic abscess involving infection with eikenella corrodens. the organism proved difficult to isolate and would not have been covered by some empirical regimens used in penicillin-allergic patients.19968842995
emergence of high rates of antimicrobial resistance among viridans group streptococci in the united states.three hundred fifty-two blood culture isolates of viridans group streptococci obtained from 43 u.s. medical centers during 1993 and 1994 were characterized. included were 48 isolates of "streptococcus milleri," 219 s. mitis isolates, 29 s. salivarius isolates, and 56 s. sanguis isolates. high-level penicillin resistance (mic, > or = 4.0 micrograms/ml) was noted among 13.4% of the strains; for 42.9% of the strains, penicillin mics were 0.25 to 2.0 micrograms/ml (i.e., intermediate resistance). in ...19968849246
false positive detection of group a streptococcus antigen resulting from cross-reacting streptococcus intermedius (streptococcus milleri group). 19968858682
the bacteriology of hidradenitis suppurativa.bacteria have been suggested as a possible cause of hidradenitis. different species have been found in several small studies, and particularly the presence of streptococcus milleri has been linked to disease activity.19968944341
[streptococcus milleri subacute spinal cord abscess. apropos of a case].since 1830, more than eighty cases of spinal cord abscesses have been reported. in half of these cases, it was unfortunately a post-mortem diagnosis. magnetic resonance imaging (mri) now gives new possibilities in the diagnosis and treatment of this rare pathology. we report here a case of cervical spinal cord abscess due to streptococcus milleri. the clinical and laboratory findings, the mri features, the pathogenicity of the offending pathogen and the surgical approach are discussed and compar ...19968952904
immunochemical characterization of the carbohydrate antigens of serotype k and lancefield group g "streptococcus milleri".carbohydrate antigens of the serotype k/lancefield group g "streptococcus milleri" were extracted by autoclaving whole cells of the type k reference strain streptococcus anginosus k214-2k. the type k and group g antigen molecules are separated from each other and partially purified by a deae-sephadex a25 column chromatography followed by a sephadex g-100 gel filtration. in the double diffusion and the immunoelectrophoresis, the type k and group g antigen preparations obtained yielded single band ...19968604251
comparison of in vitro activity of azithromycin and ampicillin against 31 isolates of streptococcus milleri.antibacterial action of azithromycin against 31 strains of dental infection-derived streptococcus milleri and tissue transfer of the agent were compared with ampicillin, the drug of first choice for dental infections. concentrations required to inhibit 50% of isolates(mic50) and concentrations required to kill 50% of isolates(mbc50) were 0.10 and 0.2 microgram/ml, respectively, for azithromycin. the antibacterial action of azithromycin was 4 times more potent in than ampicillin. the mbc90 for az ...19979618830
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