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measurement of glomerular filtration rate, renal plasma flow, and endogenous creatinine clearance in cheetahs (acinonyx jubatus jubatus).glomerular filtration rate (gfr), renal plasma flow (rpf), and the endogenous creatinine clearance (ccr) rate were determined in 13 captive cheetahs, acinonyx jubatus jubatus (seven females and six males, 1.5-7.5 yr of age, x = 5.02 yr), during general anesthesia with telazol and isoflurane by measuring the urinary clearances of inulin, para-aminohipppuric acid, and endogenous creatinine, respectively. methods to determine gfr, rpf, and endogenous ccr in captive cheetahs were evaluated, and the ...200415305512
the stress response to environmental change in captive cheetahs (acinonyx jubatus).the captive north american cheetah (acinonyx jubatus) population is not self-sustaining because of high prevalences of unusual diseases and poor reproductive success. cheetahs are commonly moved between zoos for breeding purposes to maintain genetic diversity within the captive population, and movement may exacerbate infertility and disease. fecal corticoids were analyzed by radioimmunoassay to measure the stress response of cheetahs to movement between facilities. fecal samples were collected f ...200415193067
chronic eosinophilic dermatitis associated with persistent feline herpes virus infection in cheetahs (acinonyx jubatus).a chronic ulcerative and eosinophilic dermatitis occurred in 20 captive cheetahs (acinonyx jubatus) with persistent feline herpes virus 1 (fhv1) infection. affected animals had erythematous, ulcerated plaques primarily on the face and forelegs in sites of contact with lachrymal and salivary secretions. the dermatitis was characterized by dense infiltrates of eosinophils and plasma cells and pseudoepitheliomatous hyperplasia. rare keratinocytes within the lesions had nuclei with marginated chroma ...200415017031
the use of reference strand-mediated conformational analysis for the study of cheetah (acinonyx jubatus) feline leucocyte antigen class ii drb polymorphisms.there is now considerable evidence to suggest the cheetah (acinonyx jubatus) has limited genetic diversity. however, the extent of this and its significance to the fitness of the cheetah population, both in the wild and captivity, is the subject of some debate. this reflects the difficulty associated with establishing a direct link between low variability at biologically significant loci and deleterious aspects of phenotype in this, and other, species. attempts to study one such region, the feli ...200414653802
prevalence of bartonella infection in wild african lions (panthera leo) and cheetahs (acinonyx jubatus).bartonella species are emerging pathogens that have been isolated worldwide from humans and other mammals. our objective was to estimate the prevalence of bartonella infection in free-ranging african lions (panthera leo) and cheetahs (acinonyx jubatus). blood and/or serum samples were collected from a convenience sample of 113 lions and 74 cheetahs captured in africa between 1982 and 2002. whole blood samples available from 58 of the lions and 17 of the cheetahs were cultured for evidence of bar ...200415135511
serosurvey of viral infections in free-ranging namibian cheetahs (acinonyx jubatus).cheetahs (acinonyx jubatus) in captivity have unusually high morbidity and mortality from infectious diseases, a trait that could be an outcome of population homogeneity or the immunomodulating effects of chronic stress. free-ranging namibian cheetahs share ancestry with captive cheetahs, but their susceptibility to infectious diseases has not been investigated. the largest remaining population of free-ranging cheetahs resides on namibian farmlands, where they share habitat with domestic dogs an ...200415137485
noninvasive monitoring of adrenocortical activity in carnivores by fecal glucocorticoid analyses.measurement of glucocorticoid metabolites in feces has become an accepted method for the noninvasive evaluation of adrenocortical activity. the objective of this study was to determine if a simple cortisol enzyme immunoassay (eia) was suitable for monitoring adrenocortical activity in a variety of carnivore species. performance of the cortisol eia was gauged by comparison to a corticosterone radioimmunoassay (ria) that has been used for measuring glucocorticoid metabolites in feces of numerous s ...200415158127
treatment with omeprazole, metronidazole, and amoxicillin in captive south african cheetahs (acinonyx jubatus) with spiral bacteria infection and gastritis.six captive cheetahs (acinonyx jubatus) with severe gastritis diagnosed by gastric endoscopy and mucosal histopathology were treated with omeprazole, metronidazole, and amoxicillin for 3 wk. endoscopic biopsies were performed before therapy, immediately after treatment, and 3, 7, and 19 mo after treatment. macroscopic appearance of the stomach, histologic scoring of gastric inflammation, and the presence or absence of spiral bacteria were recorded. spiral bacteria were absent histologically imme ...200415193068
analysis of the mitochondrial genome of cheetahs (acinonyx jubatus) with neurodegenerative disease.the complete mitochondrial genome of acinonyx jubatus was sequenced and mitochondrial dna (mtdna) regions were screened for polymorphisms as candidates for the cause of a neurodegenerative demyelinating disease affecting captive cheetahs. the mtdna reference sequences were established on the basis of the complete sequences of two diseased and two nondiseased animals as well as partial sequences of 26 further individuals. the a. jubatus mitochondrial genome is 17,047-bp long and shows a high sequ ...200415302412
validation of the 13c-urea breath test for use in cheetahs (acinonyx jubatus) with helicobacter.historically, therapeutic monitoring for prescribed eradication treatment of helicobacter in cheetahs (acinonyx jubatus) with associated gastritis has been accomplished only through endoscopic biopsies. the 13c-urea breath test (ubt) can offer an alternative to repeated biopsies for therapeutic monitoring. five male and five female cheetahs and one male sumatran tiger (panthera tigris) were studied. all were clinically healthy before and after this investigation. breath samples of end-tidal expi ...200415305506
vaccine-induced protection against anthrax in cheetah (acinonyx jubatus) and black rhinoceros (diceros bicornis).institution of a policy of vaccination in endangered species with a vaccine not previously administered to it cannot be undertaken lightly. this applies even more in the case of cheetah (acinonyx jubatus) with their unusually monomorphic gene pool and the potential restrictions this places on their immune responses. however, the recently observed mortalities from anthrax in these animals in the etosha national park, namibia, made it imperative to evaluate vaccination. black rhinoceros (diceros b ...200415308358
helicobacter acinonychis: genetic and rodent infection studies of a helicobacter pylori-like gastric pathogen of cheetahs and other big cats.insights into bacterium-host interactions and genome evolution can emerge from comparisons among related species. here we studied helicobacter acinonychis (formerly h. acinonyx), a species closely related to the human gastric pathogen helicobacter pylori. two groups of strains were identified by randomly amplified polymorphic dna fingerprinting and gene sequencing: one group from six cheetahs in a u.s. zoo and two lions in a european circus, and the other group from a tiger and a lion-tiger hybr ...200414702304
coronavirus outbreak in cheetahs: lessons for sars. 200415043830
cryptococcus neoformans granuloma in the lung and spinal cord of a free-ranging cheetah (acinonyx jubatus). a clinical report and literature review.a 6-year-old, male, wild-born, free-ranging cheetah (acinonyx jubatus) was evaluated for acute onset of progressive lameness in the right hind limb. survey radiographs were unrewarding and myelography indicated an intramedullary compressive mass at the l3-l4 region. a fine needle aspirate of the lesion indicated the presence of cryptococcus organisms. necropsy confirmed the presence of granulomas (cryptococcoma) in the lung and the spinal cord (meningomyelitis) caused by cryptococcus neoformans ...200516642721
comparison of helicobacter spp. in cheetahs (acinonyx jubatus) with and without gastritis.chronic gastritis causes significant morbidity and mortality in captive cheetahs but is rare in wild cheetahs despite colonization by abundant spiral bacteria. this research aimed to identify the helicobacter species that were associated with gastritis in captive cheetahs but are apparently commensal in wild cheetahs. helicobacter species were characterized by pcr amplification and sequencing of the 16s rrna, urease, and caga genes and by transmission electron microscopy of frozen or formalin-fi ...200515634976
an ancillary tool for the diagnosis of amyloid a amyloidosis in a variety of domestic and wild animals.immunohistochemistry, the standard method for diagnosing amyloid a (aa) amyloidosis, is limited in animals because it requires a large array of animal-specific anti-aa antibodies, not commercially available. the shtrasburg method (sh method) is a highly specific and sensitive technique, helping in the diagnosis and determination of aa amyloidosis in humans. the aim of this study is to determine whether the sh method is applicable in the diagnosis of aa amyloidosis in a variety of animals. tissue ...200515753466
phylogenetic studies of pantherine cats (felidae) based on multiple genes, with novel application of nuclear beta-fibrinogen intron 7 to carnivores.the pantherine lineage of the cat family felidae (order: carnivora) includes five big cats of genus panthera and a great many midsized cats known worldwide. presumably because of their recent and rapid radiation, the evolutionary relationship among pantherines remains ambiguous. we provide an independent assessment of the evolutionary history of pantherine lineage using two complete mitochondrial (mt) genes (nd2 and nd4) and the nuclear beta-fibrinogen intron 7 gene, whose utility in carnivoran ...200515804417
promoter trapping in lotus japonicus reveals novel root and nodule gus expression domains.agrobacterium-based transformation was used to introduce a promoter-less glucuronidase uida gene (beta-glucuronidase; gus) into lotus japonicus. transgenic plants were screened for gus activation at different stages after inoculation with its symbiont, mesorhizobium loti. functional gus fusion frequencies ranged from about 2 to 5% of the total number of transgenic lines. these lines provide excellent histological markers for tissue ontogeny analysis. some of the activations generated gus express ...200515899881
seroprevalence and genomic divergence of circulating strains of feline immunodeficiency virus among felidae and hyaenidae species.feline immunodeficiency virus (fiv) infects numerous wild and domestic feline species and is closely related to human immunodeficiency virus (hiv) and simian immunodeficiency virus (siv). species-specific strains of fiv have been described for domestic cat (felis catus), puma (puma concolor), lion (panthera leo), leopard (panthera pardus), and pallas' cat (otocolobus manul). here, we employ a three-antigen western blot screening (domestic cat, puma, and lion fiv antigens) and pcr analysis to sur ...200515956574
evolution of the extinct sabretooths and the american cheetah-like cat. 200516085477
pseudogenization of a sweet-receptor gene accounts for cats' indifference toward sugar.although domestic cats (felis silvestris catus) possess an otherwise functional sense of taste, they, unlike most mammals, do not prefer and may be unable to detect the sweetness of sugars. one possible explanation for this behavior is that cats lack the sensory system to taste sugars and therefore are indifferent to them. drawing on work in mice, demonstrating that alleles of sweet-receptor genes predict low sugar intake, we examined the possibility that genes involved in the initial transducti ...200516103917
extrinsic factors significantly affect patterns of disease in free-ranging and captive cheetah (acinonyx jubatus) populations.the cheetah (acinonyx jubatus) has been considered a paradigm for disease vulnerability due to loss of genetic diversity. this species monomorphism has been suspected to be the basis for their general poor health and dwindling populations in captivity. north american and south african captive populations have high prevalences of hepatic veno-occlusive disease, glomerulosclerosis, gastritis, and systemic amyloidosis, diseases that are rare in other species. unusually severe inflammatory reactions ...200516244064
efficacy and long-term outcome of gastritis therapy in cheetahs (acinonyx jubatus).a prospective clinical trial evaluating efficacy and long-term outcome of treatments for lymphoplasmacytic gastritis in cheetahs (acinonyx jubatus) was conducted. the study evaluated efficacy of 11 different antibiotic and antiinflammatory treatment protocols in 32 cheetahs (19 male, 13 female) for reducing gastric inflammation and helicobacter colonization and monitored the course of disease through histologic grading of gastric biopsies. all cheetahs were biopsied up to i wk before treatment a ...200517312757
radiologic anatomy of the normal appendicular skeleton of the lion (panthera leo). part 1: thoracic limb.thoracic limb specimens from 12 euthanized free-ranging lions (panthera leo, 16-170 mo old) underwent radiographic evaluation. the radiographic anatomy was described but excluded any areas of the bones with possible bone pathology. comparisons between adult and juvenile lions were made and physes described. differences or similarities (or both) to cheetahs (acinonyx jubatus) and domestic cats and dogs were also noted. no dissections were made to corroborate the anatomic observations, but compari ...200517315453
validating a commercially available enzyme immunoassay for the determination of 17beta-estradiol and progestogens in the feces of cheetahs (acinonyx jubatus): a case report.fecal 17beta-estradiol and progestogens excretion was monitored in adult, female cheetahs (acinonyx jubatus; n = 2), zgg-12301 (born 3 april 1993), gonadotrophin treated and zgt-3301, (born 19 august 1993), nontreated, for 120 days using commercially available plate enzyme immunoassay kits prepared for human serum or plasma. there were significant differences (p < 0.001) between baseline and peak concentrations of both hormone measures. female zgg-12301, which conceived, but this pregnancy resul ...200517315457
radiographic kidney measurements in captive cheetahs (acinonyx jubatus).the prevalence of chronic renal disease is substantial among captive cheetahs (acinonyx jubatus). the purpose of this study was to determine kidney measurements from radiographs of captive cheetahs (n = 15) with normal renal function. the ratio of kidney length to length of the body of the second lumbar vertebrae has been established for domestic cats with normal renal function. the mean ratio of renal length to length of the second lumbar vertebra was 1.81 +/- 0.14 in cheetahs. this baseline da ...200517323577
making sense of memory.the current work explores how people make recognition and belief judgments in the presence of obvious repetition primes. in two experiments, subjects received a 200-ms prime ("cheetah"), either before or after reading a trivia question ("what is the fastest animal?") but always before being presented with the target answer ("cheetah"). results showed that repetition priming decreased "old" claims (recognition--experiment 1), while it increased truth claims (belief--experiment 2). furthermore, re ...200516248499
plagues and adaptation: lessons from the felidae models for sars and aids.research studies of infectious disease outbreaks in wild species of the cat family felidae have revealed unusual details regarding forces that shape population survival and genetic resistance in these species. a highly virulent feline coronavirus epidemic in african cheetahs, a disease model for human sars, illustrates the critical role of ancestral population genetic variation. widespread prevalence of species specific feline immunodeficiency virus (fiv), a relative of hiv-aids, occurs with lit ...200632226081
analysis of amyloid fibrils in the cheetah (acinonyx jubatus).recently, a high prevalence of amyloid a (aa) amyloidosis has been documented among captive cheetahs worldwide. biochemical analysis of amyloid fibrils extracted from the liver of a japanese captive cheetah unequivocally showed that protein aa was the main fibril constituent. further characterization of the aa fibril components by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and western blot analysis revealed three main protein aa bands with approximate molecular weights ...200616911963
cardiopulmonary effects of medetomidine or midazolam in combination with ketamine or tiletamine/zolazepam for the immobilisation of captive cheetahs (acinonyx jubatus).captive cheetah (acinonyx jubatus) scheduled for either general health examination or dental surgery were immobilised with combinations of medetomidine-ketamine (k/det, n = 19), midazolam-ketamine (k/mid, n = 4) or medetomidine-tiletamine-zolazepam (z/det, n = 5). induction time and arterial blood pressure was not statistically significantly (p > 0.05) different between treatment groups. transient seizures were observed in the k/det treated animals during induction. hypertension was present in a ...200617458346
seroprevalences of antibodies to neospora caninum and toxoplasma gondii in zoo animals.neospora caninum is an apicomplexan parasite that causes neuromuscular disease in dogs and abortions in cattle. little is known about the prevalence of antibodies to this parasite in zoo animals. sera from 556 animals, from 13 czech and slovak zoos were tested for antibodies to n. caninum and toxoplasma gondii by indirect fluorescent antibody test. antibodies to n. caninum were found in 31 of 556 zoo animals (5.6%), representing 18 of 114 species tested: eurasian wolf (canis lupus lupus), maned ...200616387445
cryopreservation of spermatozoa from wild-born namibian cheetahs (acinonyx jubatus) and influence of glycerol on cryosurvival.sperm cryopreservation is a valuable tool for the genetic management of ex situ populations. this study was conducted to assess: (1) semen characteristics of wild-born cheetahs; and (2) the impact of three types of glycerol influence (duration of exposure, temperature, and method of addition) on sperm cryosensitivity. to evaluate the impact of duration of glycerol exposure, spermatozoa were incubated in test yolk buffer (tyb) with 4% glycerol at ambient temperature (approximately 22 degrees c) f ...200616412415
repeated use of the gnrh analogue deslorelin to down-regulate reproduction in male cheetahs (acinonyx jubatus).the gnrh analogue deslorelin, as a subcutaneous implant, was initially developed in australia as an ovulation-inducing agent in mares. its uses, for the suppression of reproduction in the domestic dog and cat and in other species, including humans, have been developed subsequently. such implants have been used as a contraceptive modality in a variety of wild carnivores, both males and females. this paper describes the use of deslorelin implants as a contraceptive agent for cheetah males maintain ...200616507319
elemental sulfur identified in urine of cheetah, acinonyx jubatus.the urine of the cheetah, acinonyx jubatus, is almost odorless, and probably for this reason, it has not attracted much attention from scientists. using gas chromatography-mass spectrometry, we identified 27 and 37 constituents in the headspace vapor of the urine of male and female cheetah, respectively. these constituents, composed of hydrocarbons, short-chain ethers, aldehydes, saturated and unsaturated cyclic and acyclic ketones, 2-acetylfuran, dimethyl disulfide, dimethyl sulfone, phenol, my ...200616541335
pharmaceutical prospects of phytoestrogens.interest in the physiologic and pharmacologic role of bioactive compounds present in plants has increased dramatically over the last decade. of particular interest in relation to human health are the classes of compounds known as the phytoestrogens, which embody several groups of non-steroidal estrogens, including isoflavones and lignans that are widely distributed within nature. the impact of dietary phytoestrogens on normal biologic processes was first recognized in sheep. observations of shee ...200616543667
analysis of genetic mutations in the 7a7b open reading frame of coronavirus of cheetahs (acinonyx jubatus).to analyze the 7a7b genes of the feline coronavirus (fcov) of cheetahs, which are believed to play a role in virulence of this virus.200616579755
pet-blot analysis contributes to bse strain recognition in c57bl/6 mice.identification of the strain of agent responsible for bovine spongiform encephalopathy (bse) can be made histologically through the analysis of both distribution and intensity of brain vacuolar lesions after bse transmission to mouse. another useful way to distinguish the bse agent from other prion strains is the study of the distribution of the abnormal prion protein (prp(res)). for that purpose, paraffin-embedded tissue blot (pet-blot) method was applied on brains from c57bl/6 mice infected wi ...200616735593
isolation of malassezia spp. from cerumen of wild felids.the objective of this study was to determine the presence of different species of the genus malassezia in the healthy external auditory canal of wild felids maintained in captivity. one hundred and thirty-two adult animals (264 samples of cerumen), 77 males (58.3%) and 55 females (41.7%), were studied: large felids (55 animals) - 26 lions (panthera leo), 13 tigers (panthera tigris), 6 leopards (panthera pardus), 6 jaguars (panthera onca), 2 cheetahs (acinonyx jubatus), 2 pumas (puma concolor); s ...200616772235
who ate whom? adaptive helicobacter genomic changes that accompanied a host jump from early humans to large felines.helicobacter pylori infection of humans is so old that its population genetic structure reflects that of ancient human migrations. a closely related species, helicobacter acinonychis, is specific for large felines, including cheetahs, lions, and tigers, whereas hosts more closely related to humans harbor more distantly related helicobacter species. this observation suggests a jump between host species. but who ate whom and when did it happen? in order to resolve this question, we determined the ...200616789826
independent variation of retinal s and m cone photoreceptor topographies: a survey of four families of mammals.in mammals, cone photoreceptor subtypes are thought to establish topographies that reflect the species-relevant properties of the visual environment. middle- to long-wavelength-sensitive (m) cones are the dominant population and in most species they form an area centralis at the visual axis. short-wavelength-sensitive (s) cone topographies do not always match this pattern. we here correlate the interrelationship of s and m cone topographies in representatives of several mammalian orders with dif ...200616961976
immunohistochemical screening for viral agents in cheetahs (acinonyx jubatus) with myelopathy.numerous cases of acute-onset progressive ataxia, hindlimb paresis and paralysis of unknown aetiology occurred during 1993 to 2003 in cheetahs (acinonyx jubatus) within the european endangered species programme (eep). this study describes the immunohistochemical investigation of a possible viral aetiology of the "cheetah myelopathy". antibodies to feline herpesvirus type 1, canine distemper virus, canine parvovirus and borna disease virus were applied to formalin-fixed and paraffin-embedded brai ...200617056652
bartonella infection in domestic cats and wild felids.bartonella are vector-borne, fastidious gram-negative bacteria causing persistent bacteremia in their reservoir hosts. felids represent a major reservoir for several bartonella species. domestic cats are the main reservoir of b. henselae, the agent of cat-scratch disease. prevalence of infection is highest in warm and humid climates that are optimal for the survival of cat fleas, as fleas are essential for the transmission of the infection. flea feces are the likely infectious substrate. prevale ...200617114749
the use of a probiotic in captive cheetahs (acinonyx jubatus).juvenile captive cheetahs (acinonyx jubatus) often present with diarrhoea that is commonly associated with bacterial infections. a species-specific probiotic containing lactobacillus group 2 and enterococcus faecium was prepared from healthy adult cheetahs. juvenile cheetahs (n = 27) between 8 and 13 months of age were included in the probiotic trial. the animals were observed prior to and after feeding of the probiotic which was made available for 28 days. feeding of the probiotic resulted in a ...200617137052
interspecific scaling of the morphology and posture of the limbs during the locomotion of cats (felidae).for phylogenetically diverse mammals, ranging from small rodents to large ungulates, the generalization that limb erectness increases with increased size is supported by some size-dependent scaling relationships of appendicular skeletal anatomy as well as a limited number of direct observations of limb posture during locomotion. if size alone is the causal basis for different limb posture, then the erectness of limbs should increase significantly with increased size within a phylogenetically nar ...200717267650
the composition of serval (felis serval) milk during mid-lactation.milk was obtained from three captive servals. the average nutrient content was 158.3+/-44.4 g protein; 152.6+/-62.3 g fat; and 68.7+/-31.4 g lactose per kg milk. the protein fraction respectively consisted of 117.7+/-44.8 g caseins per kg milk and of 40.6+/-6.7 g whey proteins per kg milk. electrophoresis and identification of protein bands showed a similar migrating sequence of proteins as seen in cheetah and cat milk, with small differences in the beta-caseins. the lipid fraction contains 313. ...200717307374
occurrence of babesia felis and babesia leo in various wild felid species and domestic cats in southern africa, based on reverse line blot analysis.reverse line blot (rlb) is a hybridization assay that can be used to detect various blood parasites and differentiate between them. results, using the rlb, showed that babesia felis and babesia leo occurred as single or mixed infections in various felid species, but most frequently in domestic cats and lions, respectively. prevalence of infection in free-ranging cheetahs in namibia was low (7, 5%), whereas 50% of free-ranging lions in south africa and swaziland were infected. a large number (52, ...200717084029
the mitochondrial genome structure of the clouded leopard (neofelis nebulosa).the complete 16 844 bp mitochondrial genome of neofelis nebulosa has been sequenced and compared with the complete mitochondrial genomes of felis catus and the acinonyx jubatus. the base composition of the mitochondrial genome of n. nebulosa is as follows: a, 5343 bp (31.7%); c, 4441 bp (26.4%); g, 2491 bp (14.8%); t, 4569 bp (27.1%). the genome complement and the gene order of this mitochondrial genome was found to be typical of those reported for other mammals. several unusual features of this ...200717546090
challenges in cryopreserving endangered mammal spermatozoa: morphology and the value of acrosomal integrity as markers of cryo-survival.the science of cryobiology is essential to the effective, practical use of semen for assisted breeding to help manage small populations of rare wildlife species. in this review, we describe challenges associated with cryopreserving gametes from wild fauna. based on more than 25 years of experience across a diversity of mammals, it appears that the primary driving force dictating cryo-survival of a spermatozoon is its initial pre-freeze quality and morphology, especially having a morphologically ...200717644982
acute disseminated toxoplasmosis in a juvenile cheetah (acinonyx jubatus).a juvenile cheetah (acinonyx jubatus) died with rapidly progressive pyrexia, tachypnea, abdominal effusion, and hepatomegaly. postmortem examination revealed lesions consistent with acute disseminated infection with toxoplasma gondii. the presence of this organism was confirmed in multiple organs by immunohistochemistry and polymerase chain reaction. to the best of our knowledge, we propose this to be the first reported case of primary acute disseminated toxoplasmosis in a cheetah.200717939359
induction of murine aa amyloidosis by various homogeneous amyloid fibrils and amyloid-like synthetic peptides.we investigated amyloid-enhancing factor (aef) activity of amyloid fibrils extracted from amyloid-laden livers of mice, cow, cheetah, cat and swan. all amyloid fibrils were confirmed to be amyloid protein a (aa) by an immunohistochemical analysis. we found that these fibrils accelerated the deposition of amyloid in an experimental mouse model of aa amyloidosis. furthermore, the degree of deposition was dependent on the concentration of fibrils. when we compared the minimal concentration of amylo ...200717953525
comparison of several types of enrichment for captive felids.enrichment can increase the complexity of the captive environment and possibly enhance captive animals' well-being by stimulating active behaviors and reducing stereotypical behaviors commonly seen in zoo felids. in this study, three different enrichment items were added to outdoor enclosures of felids at the montgomery zoo to test their effects on activity levels and stereotypic pacing. bones, frozen fish, and spices (cinnamon, chili powder, and cumin) were presented over a 3-month period to si ...200719360587
ejaculate traits in the namibian cheetah (acinonyx jubatus): influence of age, season and captivity.the objective was to examine the influence of animal age, season and captivity status on seminal quality in wild-born cheetahs (acinonyx jubatus) in namibia, africa. animals were divided into three age categories: juvenile (14-24 months; n = 16 males, 23 ejaculates); adult (25-120 months; n = 76 males, 172 ejaculates); and aged (>120 months; n = 5 males, 5 ejaculates). seasons were categorised into hot-wet (january-april), cold-dry (may-august) and hot-dry (september-december). a comparison betw ...200717257524
acth stimulation test in the captive cheetah (acinonyx jubatus).serum cortisol response was assessed in 8 captive cheetahs, of varying ages, after the intravenous administration of 500 microg of tetracosactide (synacthen depot, novartis, kempton park) while maintained under general anaesthesia. in addition, 8 cheetahs were anaesthetised and given an equal volume of saline in order to establish baseline cortisol concentrations at similar stages of anaesthesia. a significant difference in the median cortisol concentration measured over time was found following ...200718237035
ileocecocolic strictures in two captive cheetahs (acinonyx jubatus jubatus).intestinal strictures were diagnosed in two captive cheetahs (acinonyx jubatus jubatus). the cheetahs presented with lethargy, anorexia, diarrhea, and weight loss. the first cheetah had a stricture of the ileocecocolic junction diagnosed at necropsy. the second had an ileocecocolic stricture causing obstruction that was diagnosed at surgery. after resection and anastomosis, the cheetah recovered well. the etiology of the strictures remains undetermined. intestinal stricture, particularly of the ...200718229863
genetic analysis reveals promiscuity among female cheetahs.cheetahs (acinonyx jubatus) have a combination of ranging patterns and social system that is unique in mammals, whereby male coalitions occupy small territories less than 10% of the home range of solitary females. this study uses non-invasive genetic sampling of a long-term study population of cheetah in the serengeti national park in tanzania to infer the mating system. individual cheetah genotypes at up to 13 microsatellite loci were obtained from 171 faecal samples. a statistical method was a ...200717535795
earth science: an indian cheetah. 200717943110
family effects on early survival and variance in long-term reproductive success of female cheetahs.1. while it is generally accepted that the survival of offspring within families may be correlated, the extent of correlation has been largely untested. furthermore, the impact of such correlation on the estimated variance in females' reproductive success has rarely been quantified. 2. here we use an exceptional data set from a long-term study of individually recognized cheetahs from the serengeti national park in tanzania to formally quantify family effects in carnivores. 3. we show (i) that cu ...200717714269
relating long-term studies to conservation practice: the case of the serengeti cheetah project.although detailed, long-term scientific studies provide potentially crucial information for conservation, they are rare. moreover, there is often a disjunction between scientists and managers that can affect whether scientific results are applied to help solve conservation problems. long-term studies can promote increased communication between scientists and managers and hence offer an opportunity for constructive engagement between the two groups. we examined direct and indirect impacts of a 30 ...200717531039
electrophysiological correlates of perceptual reversals for three different types of multistable images.electrophysiological recordings were made in 21 observers to investigate whether differences in signature components (p1, n1, selection negativity [sn]) would be revealed during perceptual reversals of three different multistable figures. using a lattice of necker cubes as a stimulus, j. kornmeier and m. bach (2004, 2005) reported differences in p1 amplitudes as well a broad reversal-related negativity occurring 200-400 ms poststimulus. the current study investigated whether these event-related ...200717461674
nutrient digestibility and fecal characteristics are different among captive exotic felids fed a beef-based raw diet.nutrient digestibility has not been well characterized in exotic felids. the objective of this experiment was to evaluate differences in nutrient digestibility and fecal characteristics in five large exotic captive felid species, including bobcats, jaguars, cheetahs, indochinese tigers, and siberian tigers. all animals were individually housed and adapted to a beef-based raw diet (nebraska brand((r)) special beef feline, north platte, ne) for 16 d. total fecal collections were conducted from day ...200819360610
[ultrasound findings in spontaneous and postoperative anal pain].to assess the use of endoanal ultrasounds to identify anal pain etiology in patients with either spontaneous or post-operative pain, and to review the most frequent causes.200819222335
captive breeding of cheetahs in south africa--30 years of data from the de wildt cheetah and wildlife centre.the de wildt cheetah and wildlife centre was established in 1971 and the first cheetah cubs were born in 1975. during the period 1975-2005, 242 litters were born with a total of 785 cubs. mean cub survival from 1 to 12 months and greater than 12 months of age was 71.3 and 66.2%, respectively. the majority of losses (84.9%) occurred during the first month postpartum whereas only 15.1% deaths took place between 1 and 12 months of age. females were first bred at an age of approximately 3 years, rea ...200818638106
are cheetahs on the run from prion-like amyloidosis? 200818487456
some properties of explosive mixtures containing peroxides part i. relative performance and detonation of mixtures with triacetone triperoxide.this study concerns mixtures of triacetone triperoxide (3,3,6,6,9,9-hexamethyl-1,2,4,5,7,8-hexoxonane, tatp) and ammonium nitrate (an) with added water (w), as the case may be, and dry mixtures of tatp with urea nitrate (un). relative performances (rp) of the mixtures and their individual components, relative to tnt, were determined by means of ballistic mortar. the detonation energies, e0, and detonation velocities, d, were calculated for the mixtures studied by means of the thermodynamic code ...200818023972
neoplasia in felids at the knoxville zoological gardens, 1979-2003.a review of medical records and necropsy reports from 1979-2003 found 40 neoplasms in 26 zoo felids, including five lions (panthera leo, two males and three females), three leopards (panthera pardus, two males and one female), one jaguar (panthera onca, female), 11 tigers (panthera tigris, three males and eight females), two snow leopards (panthera uncia, one male and one female), two cougars (felis concolor, one male and one female), one bobcat (felis rufus, male), and one cheetah (acinonyx jub ...200819110704
ulnar metaphyseal osteochondrosis in seven captive bred cheetahs (acinonyx jubatus).distal ulna metaphyseal osteochondrosis was identified in seven captive bred cheetahs raised in australia between 1984 and 2005. the disorder was characterized by bilateral carpal valgus conformation. in the metaphyseal region of the distal ulnae, an osteolucent defect that appeared as a proximal extension of the lucent physis was identified radiographically between 6 and 10 months of age. ulna ostectomy was done to correct the angular limb deformity. histologically, changes were identified in t ...200819051644
characterization of the cheetah serum amyloid a1 gene: critical role and functional polymorphism of a cis-acting element.amyloid a (aa) amyloidosis is one of the principal causes of morbidity and mortality in captive cheetahs (acinonyx jubatus), which are in danger of extinction. for practical conservation of this species, therefore, it is critical to elucidate the etiology of aa amyloidosis, especially to understand the mechanisms of transcriptional regulation of serum amyloid a (saa), a precursor protein of the aa protein. in this study, the structure and nucleotide sequence of the cheetah saa1 gene including th ...200818375929
comparative thermoregulation and the quest for athletic supremacy.there are a number of different strategies used by animals to effectively deal with the changing environment. the various thermoregulatory strategies employed by mammals can be a critical factor determining the survival and physical performance in a range of conditions. however, it is not readily appreciated that mammals regulate their body temperature in different ways and it is usually assumed that the mechanisms for temperature regulation are very similar amongst all endotherms. in this chapt ...200819208996
molecular genetic insights on cheetah (acinonyx jubatus) ecology and conservation in namibia.the extent and geographic patterns of molecular genetic diversity of the largest remaining free-ranging cheetah population were described in a survey of 313 individuals from throughout namibia. levels of relatedness, including paternity/maternity (parentage), were assessed across all individuals using 19 polymorphic microsatellite loci, and unrelated cheetahs (n = 89) from 7 regions were genotyped at 38 loci to document broad geographical patterns. there was limited differentiation among regions ...200817982159
predator-prey size relationships in an african large-mammal food web.1. size relationships are central in structuring trophic linkages within food webs, leading to suggestions that the dietary niche of smaller carnivores is nested within that of larger species. however, past analyses have not taken into account the differing selection shown by carnivores for specific size ranges of prey, nor the extent to which the greater carcass mass of larger prey outweighs the greater numerical representation of smaller prey species in the predator diet. furthermore, the top- ...200818177336
cheetahs of the deep sea: deep foraging sprints in short-finned pilot whales off tenerife (canary islands).1. empirical testing of optimal foraging models for breath-hold divers has been difficult. here we report data from sound and movement recording dtags placed on 23 short-finned pilot whales off tenerife to study the foraging strategies used to catch deep-water prey. 2. day and night foraging dives had a maximum depth and duration of 1018 m and 21 min. vocal behaviour during dives was consistent with biosonar-based foraging, with long series of echolocation clicks interspersed with buzzes. simila ...200818444999
fecal transmission of aa amyloidosis in the cheetah contributes to high incidence of disease.aa amyloidosis is one of the principal causes of morbidity and mortality in captive cheetahs (acinonyx jubatus), which are in danger of extinction, but little is known about the underlying mechanisms. given the transmissible characteristics of aa amyloidosis, transmission between captive cheetahs may be a possible mechanism involved in the high incidence of aa amyloidosis. in this study of animals with aa amyloidosis, we found that cheetah feces contained aa amyloid fibrils that were different f ...200818474855
seroepidemiology of toxoplasma gondii in zoo animals in selected zoos in the midwestern united states.toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to t. gondii oocysts excreted by cats in the zoos. seroprevalence of t. gondii antibodies in wild zoo felids, highly susceptible zoo species, and feral cats from 8 zoos of the midwestern united states was determined by using the modified agglutination test (mat). a titer of 1:25 was considered indicative of t. ...200818605803
refinement of a commercial bench-top relaxin assay for pregnancy diagnosis using urine from domestic and nondomestic felids.relaxin, a 6-kda polypeptide hormone, is excreted in the urine during pregnancy in several mammalian species. a recent study showed that detection of urinary relaxin using a bench-top serum assay (witness relaxin kit, synbiotics corp., san diego, california 92127, usa) can be diagnostic for pregnancy in domestic cats (felis silvestris catus), but it is unknown whether the bench-top kit is applicable with urine across felid species. our objectives were to 1) examine modifications in urine process ...200818634207
naturally acquired anthrax antibodies in a cheetah (acinonyx jubatus) in botswana.an outbreak of anthrax in the jwana game reserve in jwaneng, botswana, was first observed when three cheetahs (acinonyx jubatus) died of the disease in november 2004. in the aftermath of this event, banked serum samples collected from 23 wild-caught cheetahs were examined, by the inhibition enzyme-linked immunoassay (elisa), for antibodies to the protective antigen (pa) of bacillus anthracis. of the 23 cheetahs, 16 regularly accessed the reserve. antibodies to pa were detected in one cheetah col ...200818689661
molecular detection of babesia rossi and hepatozoon sp. in african wild dogs (lycaon pictus) in south africa.blood specimens from wild dogs (n=301) were obtained from de wildt cheetah and wildlife centre (pretoria) and five game reserves (4 in the north-west province and 1 in limpopo province), south africa. specimens were screened for babesia, theileria, hepatozoon and ehrlichia/anaplasma species using pcr and reverse line blot (rlb) assays. positive results were obtained in 18 (6%) wild dogs. sixteen specimens were found positive for babesia rossi and two dogs were hepatozoon sp. positive. it appears ...200818752897
motions of the running horse and cheetah revisited: fundamental mechanics of the transverse and rotary gallop.mammals use two distinct gallops referred to as the transverse (where landing and take-off are contralateral) and rotary (where landing and take-off are ipsilateral). these two gallops are used by a variety of mammals, but the transverse gallop is epitomized by the horse and the rotary gallop by the cheetah. in this paper, we argue that the fundamental difference between these gaits is determined by which set of limbs, fore or hind, initiates the transition of the centre of mass from a downward- ...200918854295
a primitive late pliocene cheetah, and evolution of the cheetah lineage.the cheetah lineage is a group of large, slender, and long-limbed cats with a distinctive skull and dental morphology, of which only the extant cheetah (acinonyx jubatus) is present today. the lineage is characterized by having abbreviated, tall, and domed crania, and a trenchant dentition with a much reduced, posteriorly placed protocone on the upper carnassial. in this article, we report on a new discovery of a late pliocene specimen from china with an estimated age of approximately 2.2-2.5 mi ...200919114651
characterization of an outbreak of astroviral diarrhea in a group of cheetahs (acinonyx jubatus).a mamastrovirus was identified in an outbreak of diarrhea in cheetahs (acinonyx jubatus). five young adult and two adult cheetahs presented with lethargy, anorexia, watery diarrhea and regurgitation over an 11-day period. fecal samples were submitted for electron microscopy and culture. electron microscopy results revealed particles morphologically consistent with an astrovirus, and no other viral pathogens or significant bacterial pathogens were identified. the astrovirus was confirmed and sequ ...200919171442
the complete mitochondrial genome structure of snow leopard panthera uncia.the complete mitochondrial genome (mtdna) of snow leopard panthera uncia was obtained by using the polymerase chain reaction (pcr) technique based on the pcr fragments of 30 primers we designed. the entire mtdna sequence was 16 773 base pairs (bp) in length, and the base composition was: a-5,357 bp (31.9%); c-4,444 bp (26.5%); g-2,428 bp (14.5%); t-4,544 bp (27.1%). the structural characteristics [0] of the p. uncia mitochondrial genome were highly similar to these of felis catus, acinonyx jubat ...200918431688
who's the faster runner: the cheetah or the rabbit? 200919292856
immunohistochemical study of prp(sc) distribution in neural and extraneural tissues of two cats with feline spongiform encephalopathy.two domestic shorthair cats presenting with progressive hind-limb ataxia and increased aggressiveness were necropsied and a post mortem diagnosis of feline spongiform encephalopathy (fse) was made. a wide spectrum of tissue samples was collected and evaluated histologically and immunohistologically for the presence of prpsc.200919335885
phylogeny of a novel "helicobacter heilmannii" organism from a japanese patient with chronic gastritis based on dna sequence analysis of 16s rrna and urease genes."helicobacter heilmannii" is an uncultivable spiral-shaped bacterium inhabiting the human gastric mucosa. it is larger and more tightly-coiled than h. pylori. we encountered a patient with chronic gastritis infected a "h. heilmannii"-like organism (hhlo), designated as sh6. gastric mucosa derived from the patient was orally ingested by specific pathogen free mice. colonization of the mice by sh6 was confirmed by electron microscopy of gastric tissue specimens. in an attempt to characterize sh6, ...200919412605
oral examination and radiographic evaluation of the dentition in wild cats from namibia.feline tooth resorption has been widely reported in domestic cats and sporadically described in other felidae. the goal of the present study was to determine the prevalence of tooth resorption and to report other dental problems in a population of wild felidae. observations of dental disorders and anomalies were made in skulls from 73 wild felidae (cheetahs, leopards, caracals, african wildcats, and lions) originating from namibia. in addition, radiographs were taken in 43 cases to determine sig ...200919476083
real-time reinforcement learning by sequential actor-critics and experience replay.actor-critics constitute an important class of reinforcement learning algorithms that can deal with continuous actions and states in an easy and natural way. this paper shows how these algorithms can be augmented by the technique of experience replay without degrading their convergence properties, by appropriately estimating the policy change direction. this is achieved by truncated importance sampling applied to the recorded past experiences. it is formally shown that the resulting estimation b ...200919523786
possible case of maternal transmission of feline spongiform encephalopathy in a captive cheetah.feline spongiform encephalopathy (fse) is considered to be related to bovine spongiform encephalopathy (bse) and has been reported in domestic cats as well as in captive wild cats including cheetahs, first in the united kingdom (uk) and then in other european countries. in france, several cases were described in cheetahs either imported from uk or born in france. here we report details of two other fse cases in captive cheetah including a 2(nd) case of fse in a cheetah born in france, most likel ...200919738899
advances in reproductive science for wild carnivore conservation.knowledge about reproduction is critical for predicting the viability of wildlife populations in nature and for managing breeding programmes in captivity. intensive species-based studies are the priority, because reproductive mechanisms are extraordinarily diverse, even within the same taxonomic family. carnivores deserve more attention as such species are highly vulnerable to environmental change and human persecution. the present review provides contemporary illustrations of how reproductive s ...200919754535
periaortic haemangiosarcoma in an african wild dog (lycaon pictus).a 9-year-old apparently healthy male african wild dog (lycaon pictus) was found dead in its enclosure at the de wildt cheetah and wildlife centre. necropsy revealed a pericardium distended by approximately 250 ml of thick blood. a soft, red, lobulated mass was attached to the periaortic fat between the level of the aortic valves and the pericardial reflection. histologically, the mass was consistent with a haemangiosarcoma. other findings in the heart included mild to moderate ventricular hypert ...200919831274
a comparative approach to the study of keeper-animal relationships in the zoo.research on intensively farmed animals over the past 25 years has shown that human-animal interactions, by affecting the animal's fear of humans, can markedly limit the productivity and welfare of farm animals. this article begins to explore some of the factors that need to be considered to investigate keeper-animal relationships (kars) in the zoo. in the mid-1990s, a large body of multi-institutional data on zookeepers and animals was collected from 46 zoos. using standardized questionnaires, 8 ...200919885915
improved quality of cryopreserved cheetah (acinonyx jubatus) spermatozoa after centrifugation through accudenz.sperm cryopreservation, in combination with assisted reproductive techniques, is a valuable tool for the genetic management of endangered felids. however, the acrosome of the cheetah spermatozoon is especially sensitive to cryopreservation, with approximately 40% of spermatozoa experiencing acrosomal damage immediately after thawing and then another approximately 15% loss during the next 4 hours in vitro. additionally, thawing causes a reduction in sperm motility by approximately 20% with anothe ...200919023140
energetic ethylene- and propylene-bridged bis(nitroiminotetrazolate) salts.high energy density materials with ethylene- and propylene bis(5-nitroiminotetrazolate) as the anions are reported; all salts were fully characterized by ir, and (1)h, (13)c, and (15)n nmr spectroscopy as well as elemental analyses. in addition, the heats of formation (deltah(f)) and the detonation pressures (p) and velocities (d) were calculated.the synthesis and detonation properties of high energy density materials with ethylene- and propylene bis(nitroiminotetrazolate) as the anions are repo ...200919197919
highly dense nitranilates-containing nitrogen-rich cations.high density energetic salts containing nitrogen-rich cations and the nitranilic anion were readily synthesized in high yield by metathesis reactions of sodium nitranilate 2 and an appropriate halide. all of the new compounds were fully characterized by elemental, spectral (ir, (1)h, (13)c nmr), and thermal (dsc) analyses. the structure of hydrazinium nitranilate (4) was also determined by single-crystal x-ray analysis. the high symmetry and oxygen content of the anion give these salts extensive ...200919072948
carbonyl and oxalyl bridged bis(1,5-diaminotetrazole)-based energetic salts.high density energetic salts containing nitrogen rich cations and carbonyl- or oxalylbis(diamino-tetrazole) anions, which were obtained from cyanogen azide and hydrazine, were readily synthesized. in every case, a new family of energetic salts 3-14 were characterized by vibrational spectroscopy, multinuclear ((1)h, (13)c, (15)n) nmr, elemental analyses, density, differential scanning calorimetry and impact sensitivity. compound 12 was structured by single crystal x-ray diffraction. the densities ...200919658135
cocrf deformable model: a geometric model driven by collaborative conditional random fields.we present a hybrid framework for integrating deformable models with learning-based classification, for image segmentation with region ambiguities. we show how a region-based geometric model is coupled with conditional random fields (crf) in a simple graphical model, such that the model evolution is driven by a dynamically updated probability field. we define the model shape with the signed distance function, while we formulate the internal energy with a c(1) continuity constraint, a shape prior ...200919574164
nitroimino-tetrazolates and oxy-nitroimino-tetrazolates.highly energetic 1,1'-ethylenebis(oxy)bis(5-nitroimino-tetrazolate) salts were obtained by reacting equimolar quantities of the acidic 1,1'-ethylenebis(oxy)bis(5-nitroimino-tetrazole) and energetic bases in aqueous solution. additionally, metathesis of silver 1,1'-ethylenebis(oxy)bis(5-nitroimino-tetrazolate) with diaminoguanidinium chloride or triaminoguanidinium chloride gave the corresponding oxy-nitroimino-tetrazolate salt. these salts were fully characterized using ir and multinuclear nmr s ...201020925341
3,4,5-trinitropyrazole-based energetic salts.high-density energetic salts that are comprised of nitrogen-rich cations and the 3,4,5-trinitropyrazolate anion were synthesized in high yield by neutralization or metathesis reactions. the resulting salts were fully characterized by (1)h, (13)c nmr, and ir spectroscopy; differential scanning calorimetry; and elemental analysis. additionally, the structures of the 3,5-diaminotriazolium and triaminoguanidinium 3,4,5-trinitropyrazolates were confirmed by single-crystal x-ray diffraction. based on ...201020661962
theoretical studies on the formation mechanism and explosive performance of nitro-substituted 1,3,5-triazines.to develop new highly energetic materials, we have considered the design of molecules with high nitrogen content. possible candidates include 1,3,5-triazine derivatives. in this work, we studied potential synthetic routes for melamine using the mp2/6-31+g(d,p)//b3lyp/6-31g(d) level of theory. the mechanisms studied here are stepwise mechanism beginning with the dimerization of cyanamide and one-step termolecular mechanism. the same type of mechanism is also applied to nitro-substituted 1,3,5-tri ...201020652990
neurological dysfunction and caudal fossa overcrowding in a young cheetah with hypovitaminosis a. 201021257402
exposure of growing and adult captive cheetahs (acinonyx jubatus) to dietary isoflavones: twenty years later.dietary isoflavones are associated with oestrogenic and anti-oestrogenic effects, and have been linked to infertility in cheetahs. this study aimed to determine the isoflavone content of commercially prepared diets consumed by captive cheetahs. sixteen international zoological facilities provided diets, and the isoflavone content of each diet was determined by acid hydrolysis and hplc quantification. proximate nutritional composition was also determined. over half the diets analysed contained de ...201020662971
parasites of domestic and wild animals in south africa. xlviii. ticks (acari: ixodidae) infesting domestic cats and wild felids in southern africa.ticks collected from domestic cats (felis catus), cheetahs (acinonyx jubatus),caracals (caracal caracal), african wild cats (felis lybica), black-footed cats (felis nigripes), a serval (leptailurus serval), lions(panthera leo), and leopards (panthera pardus) were identified and counted. thirteen species of ixodid ticks and one argasid tick were identified from domestic cats and 17 species of ixodid ticks from wild felids. the domestic cats and wild felids harboured 11 ixodid species in common. t ...201023327159
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