Publications

characterization of allergens prepared from smooth and rough strains of brucella melitensis.		1973	4758380
brucella melitensis rev. 1 and brucella abortus 45-20 vaccines in goats: serologic tests.		1973	4631096
brucella melitensis rev. 1 and brucella abortus 45-20 vaccines in goats: pattern of immunoglobulin production after vaccination and challenge.		1973	4631097
phenol-water fractions from smooth brucella abortus and brucella melitensis: immunochemical analysis and biologic behavior.		1973	4632040
the free lipids of brucella melitensis and bordetella pertussis.		1973	4351160
serological titres following vaccination of sheep and goats with brucella melitensis rev i vaccine.		1973	4197921
immunization against brucella infections. priming of cynomolgus philipinensis with purified antigen of brucella melitensis prior to injection of rev. i vaccine.		1973	4199459
stimulation of anti-brucella vaccination in mice by tetramisole, a phenyl-imidothiazole salt.	the effect of tetramisole (hydrochloride of racemic 2, 3, 5, 6,-tetrahydro-6-phenyl-imidazo [2, 1-b] thiazole) on immunization was investigated in mice vaccinated by killed brucella melitensis cells suspended in incomplete adjuvant. immunity to brucella abortus challenge was estimated by the reduction in number of b. abortus colonies per gram of spleen in those mice which escaped full immunization and also by calculation of mean infective doses for each group of mice. all tetramisole treatments ...	1973	4200541
epidemiology of human brucellosis in isfahan, iran.	studies on brucellosis were carried out to elucidate the epidemiology of the disease in isfahan province, where brucella melitensis is highly prevalent in animals and man. a positive milk ring test of 32% and 23% was found in unvaccinated goats and sheep respectively. card and tube agglutination tests showed an infection rate of about 12% in sheep and goats and 42% in cattle. b. melitensis was isolated from 8% of 677 samples of fresh cheese examined.of 1526 clinically suspected human cases, 476 ...	1974	4529097
brucella melitensis endocarditis: successful treatment of an infected prosthetic mitral valve.		1974	4850832
intrauterine fetal infection with brucella melitensis as a possible cause of second-trimester abortion.		1974	4857794
an unusual outbreak of brucellosis.	a brucella melitensis infection involved three persons. the infections were acquired by contact with a culture suspension that had been spilled during a laboratory exercise. one of the patients had only indirect contact with the spilled culture, probably contact with contaminated fomites. another of the patients developed a clinical remission before treatment with antibiotics and relapsed after six months. this patient also had a positive bone marrow culture when blood cultures were negative. th ...	1975	1052665
[preparation and standarization of a brucellosis allergen of sheep and goats].	the authors underline the consequences upon national economy and public health of sheep and goat brucellosis and expose the difficulties existing with the serological methods of diagnosis presently in use. in order to eliminate these disadvantages they propose the use of the intradermal allergic test reporting, at the same time, the reasons for which this test has not been until now universally adopted : lack of a standard allergen, always prepared by the same method and possessing excellent qua ...	1975	1149929
titration of a brucella protein allergen in sheep sensitized with brucella melitensis.	a protein allergen extracted from a rough strain of b. melitensis was titrated in sheep sensitized by vaccination with the attenuated b. melitensis strain rev. i. single doses of allergen were given per animal by the intradermal or palpebral routes. doses over a 100-fold range produced delayed hypersensitivity reactions of similar magnitude by both routes. the most suitable method for application in the field was a dose of 50 mug given by the palpebral route (0.5 ml subcutaneously in the upper e ...	1975	1163961
brucellosis outbreak attributed to ingestion of umpasteurized goat cheese.	in six cases of brucella melitensis infections the common etiologic factor was the ingestion of raw goat cheese. presenting complaints were variable, but a constant feature was persistent fevers that frequently were present for months. evidence of liver cell dysfunction was present in each case, and in two, biopsy disclosed diffuse hepatitis with focal necrosis. the diagnoses were initially based on high titers of brucella agglutinins and later confirmed by positive blood cultures. a good clini ...	1975	1170820
brucellosis linked to mexican cheese.	three cases of brucellosis occurred, two acute cases and one chronic, all of which likely resulted from the ingestion of fresh cheese containing brucella melitensis. a history of patients with illness suggestive of brucellosis should include details about food ingested recently in addition to the usual questions about travel and occupation. as the us domestic-animal disease comes under better control, and travel to mexico, spain, and italy increases, it seems likely that human disease caused by ...	1975	1173155
[the intracellular multiplication of "brucella" the theory for an experimental model (author's transl)].	brucella melitensis can penetrate and multiply in cells of the bhk21 line. the intracellular bacteria can be counted either by culture after destruction of the cells or by direct examination after staining; in the latter case, the cells are distributed into various classes according to the number of bacteria which they contain. the number of intracellular bacteria increased exponentially and the division time is 12 hours. the intracellular division of the bacteria is independent of the cellular ...	1975	1200552
[nitrofurantoin-test for the differentiation of bordetella bronchiseptica (author's transl)].	bordetella bronchiseptica is primarily resistant against nitrofurantoin (mic greater than 200 mug/ml), and this feature can be used for the differentiation of the organism from other gram-negative coccobacteria. nitrofurantoin paper disks (300 mug) failed to affect the growth of 150 strains of b. bronchiseptica isolated from different animal hosts, but they produced marked inhibition zones in the cultures of the followingspecies: pasteurella multocida, pasteurella pneumotropica, pasteurella haem ...	1975	809944
[development of spleenic infection in the mouse following intravenous inoculation of various species of brucella in smooth (s) or rough (r) phases].	the bacterial population in the spleen of mice inoculated intravenously by brucella abortus 544 (s), brucella suis 1330 (s), brucella melitensis 115 (r) or brucella canis rm 666(r) is studied kinetically. like the s strains of brucella, the r strains are able to multiply in the mouse spleen. the consequences for the tests of vaccines against natural infections produced by s or r strains of brucella are discussed.	1975	810263
evolution and taxonomy in the genus brucella: steroid hormone induction of filterable forms with altered characteristics after reversion.	several strains of brucella abortus, brucella suis, and brucella melitensis were exposed to physiologic concentrations of testosterone, progesterone, and diethylstilbestrol by incorporating them into the growth medium. the hormones induced brucella to form cell wall-defective organisms, including filterable forms. the filterable forms from 1 strain of b melitensis had altered characteristics when it reverted to growth as an intact cell. this is the 1st reported instance of induction of filterabl ...	1976	816232
priming of macaca cynomolgus philippinensis with purified antigen of brucella melitensis before injection of rev. i vaccine.	in experiments designed for study of bacteremia and serologic response of macaca cynomolgus philippinensis to vaccination with a soluble antigen (fraction i) of brucella melitensis and the live rev. i vaccine strain, fraction i and rev. i were administered both four weeks apart and simultaneously. one of three monkeys given the vaccines four weeks apart and two of three monkeys vaccinated with fraction i and rev. i in a single injection developed transient bacteremia, as shown by blood cultures. ...	1976	824373
relation between macrophage migration inhibition and immunity to brucella abortus in guinea-pigs.	relation between macrophage migration inhibition and immunity to brucella abortus in guinea-pigs. onderstepoort journal of veterinary research 43 (4), 175-184 (1976). a soluble protein antigen was prepared from brucella melitensis rev i with which macrophage migration inhibition (mmi) assays were successfully done using guinea-pig peritoneal exudate cells. by comparing the mmi, agglutinin response and immunity of groups and of individual guinea-pigs which had been immunized with either b. melite ...	1976	828715
killed vaccine in adjuvant and protection of mice against an intraperitoneal challenge of brucella: kinetic studies.	cd-i female mice were immunized with a subcutaneous injection of heat-inactivated brucella melitensis (strain 53 h 38) incorporated into a water-in-oil emulsion. one month later, the effectiveness of this immunization was investigated by studying quantitatively in the spleen the fate of an intraperitoneal challenge inoculum of approximately i x 10(6) viable b. abortus strain 544. in unvaccinated mice, the number of viable challenge bacteria increased until about the 10th day, decreased and then ...	1976	829301
[cachexia in mice induced by a fraction of brucella melitensis. modification by levamisole or by diethyldithiocarbamate of soda].	the pmf fraction of b. melitensis creates a wasting disease in newborn mice (j. infect. dis., 1973, 127, p. 139). administered to adult mice, pmf induces a 4 to 8 g loss of weight in 20 g mice and more markedly in males than in females. treatment with levamisole (lms) suppresses this difference in response to pmf and restores normal weight in 24 days. diethyldithiocarbamate (ddc), another immunostimulant sulfur derivate, abolishes the emaciating effect of pmf. furthermore, ddc is an anabolizing ...	1976	177323
[preparation and testing of different antibrucellic vaccines].	from a highly virulent brucella melitensis strain of human origin, different vaccines of good antigenic quality are obtained, ranging from the very agglutinogenic oily type vaccine to the totally non-agglutinogenic oily type vaccine. the non-agglutinogenic vaccine is obtained by an original method consisting of submission of the original brucella to several subcultures in the presence of a small quantity of antiserum and clonal selection of a population. the strain finally selected is totally no ...	1976	1261748
brucellosis as a world problem.	brucellosis is one of the most widespread and economically the most ravaging of zoonoses. the occurrence of the acute, often incapacitating infection in man caused by brucella melitensis usually coincides with occurrence of the infection in sheep and goats. well-known foci of this infection have been identified in the mediterranean basin, central asia and latin america. recent investigations in parts of africa and india have shown that the infection is much more widespread than was previously su ...	1976	1261753
[chemical fractionation of brucella melitensis and the immunizing properties of a new fraction].	a complex phenol-insoluble fraction (fraction p.i.) extracted from brucella melitensis presents immunizing properties already described by the authors. this fraction contains peptidoglycane whose immuno-adjuvant properties are known in numerous bacterial species. more precise fractionation was carried out to determine if the properties observed after injection of p.i. are in fact specific or on the contrary due to peptidoglycane action. by chemical and enzymatic treatments, 90% of the p.i. are e ...	1976	1261754
[the genus brucella, its nomenclature and taxonomic specificity (author's transl)].	the author suggest to change the nomenclature of genus-brucella as follows: brucella melitensis, b. bovis, b. suis, b. canis, b. neotomae, b. rangiferi, b. murium. in the new edition of "bergey's manual" it is suggested to quote some highly taxonomic specific substances, berucellaphages, brucelline, protective substance, precipitinogen, and toxins (endotoxin, lipoprotein).	1976	1274499
[letter: meningo-encephalitis and polyradiculoneuritis of brucellar origin. isolation of brucella melitensis in the c.s.f].		1976	934882
human brucellosis in kenya.	the serology, epidemiologic features and clinical presentation of 39 patients suffering from brucellosis in kenya are described. the prevalent species was brucella melitensis. there was a striking preference in the localisation of the infection for the spine and the hip joint. the disease runs a protracted course. phenomena due to hypersensitivity and neuropsychiatric disorders are discussed. advice regarding specific treatment is given.	1976	941242
[immunization of ewes against experimental brucella melitensis infection: comparison of eleven vaccines].	a comparative study of 11 antibrucellic vaccines has been carried out in the limousin region of 360 ewe-lambs divided into groups of 30, a 12th group serving as control. after being vaccinated at 8 months, the ewe-lambs were covered between 9 1/2 and 10 1/2 months, then infected at 11 months with br. melitensis strain 53 h 38 by instillation of 4 x 10(6) germs on the conjonctiva. six of these vaccines reduced the number of abortions and the excretion of brucella at parturition: three consisted o ...	1976	944151
isolation of brucella melitensis phage of broad biotype and species specificity.	a brucella melitensis phage was isolated. stocks of phage were established which produced early large plaques. the phage host range included all smooth brucella species.	1976	947844
stimulating activity of brucella fractions in a human lymphocyte transformation test. correlation with humoral and cellular immunity.	'pi' a phenol-extracted fraction from brucella melitensis has been used for years in our laboratory for the vaccination of laboratory and agricultural workers considered 'at risk'. in the present work it has been used in vitro, together with another phenol-extracted and peptidoglycan containing fraction (4a) in a lymphocyte transformation test. correlation between stimulation index, humoral antibodies to brucella and the cutaneous response to pi have been studied in subjects vaccinated with the ...	1976	992708
evidence of reservior hosts of brucella mellitensis.	serological reactors to brucella melitensis were found among goats and eland in chilanga. the only clinical evidence of disease were abortion among the goats, and circumstances did not allow for isolation of the organism in the laboratory. no gross pathological lesions were observed on post mortem examination of the goats. the public health significance relates to the ease of br. melitensis transmission between reservoir hosts and human beings.	1976	1052104
immunization by an insoluble fraction extracted from brucella melitensis: immunological and chemical characterization of the active substances.	a peptidoglycan-containing fraction called fraction p.i. (phenol insoluble), extracted from brucella melitensis and previously described by some of us, had immunogenic and protective properties and did not produce any allergic reactions. since it is well known that bacterial peptidoglycans studied so far have immunoadjuvant properties, the isolation of the active factor(s) of brucella was undertaken. by successive enzymatic and chemical treatments, a new, much more purified fraction, called "4a" ...	1976	770324
[preservation of lyophilized brucella melitensis rev. 1 vaccine].		1977	279055
preliminary report on brucella melitensis, strain h-38 vaccine in cattle.		1977	286366
on the growth of brucella species in presence of erythritol in defined and undefined media and amniotic fluid of human, cow and sheep.	different concentrations of erythritol (25-1000 microng/ml) in defined medium were shown to enhance the growth of 75.00 to 91.66 per cent of brucella abortus and 69.23 to 100.00 per cent of brucella melitensis strains studied. compared to these figures only 13.04 to 60.86% (in tryptose broth), and 41.40 to 62.50% (in glycerol dextrose broth), of brucella abortus strains, 25.00 to 83.30 per cent (in tryptose broth) and 83.30 to 100.00 per cent (in glycerol dextrose broth) of brucella melitensis s ...	1977	403714
enumeration and identification of human leukemic lymphocytes by their natural binding of bacteria.	the recently described property of bacteria to bind to human lymphocytes was used to distinguish between normal and chronic leukemic lymphocyte (cll) populations. strains of the following bacteria were used in this study: arizona hinshawii, escherichia coli strains 1 and 2, bacillus globigii, brucella melitensis, corynebacterium diphtheriae strains 1 and 2, corynebacterium xerosis, sarcina lutea, staphylococcus aureus, and staphylococcus epidermidis. for identification of immunoglobulin-bearing ...	1977	404035
rifampin in the treatment of experimental brucellosis in mice and guinea pigs.	rifampin, a broad-spectrum antibiotic able to penetrate intracellularly, was used for treatment of infections with brucella melitensis in mice and brucella abortus in guinea pigs. treatments were administered for seven, 14, or 21 days; mice were given 25 mg of rifampin/kg per day, and guinea pigs 100 mg/kg per day. efficacy of the drug was determined by comparison of rifampin-treated animals with saline-treated controls and with tetracycline-treated mice (200 mg/kg per day) according to the foll ...	1977	409786
[allergic diagnosis of bovine brucellosis. 1. conditions for the use of a purified protein allergen: brucellin (author's transl)].	a protein allergen extracted from the rough strain brucella melitensis b 115, prepared for the diagnosis of brucellosis, has been evaluated in cows. injected in non infected and non vaccinated cows, this allergen does not give any reaction, does not sensitize for subsequent injections, does not give rise to antibodies detected by routine serologic tests, and thus does not interfere with usual screening. in cows, sensitized by injections of h 38 or 45/20 vaccines, the intradermal injection produc ...	1977	413466
vaccination of adult sheep with reduced doses of brucella melitensis strain rev.1: safety and serological responses.	a trial was made to test the safety of a brucella melitensis strain rev. 1 vaccine when used in various doses on pregnant sheep and a control group of pregnant goats. it was found that local sheep were more susceptible than goats to the effects of this vaccine, and that the accepted dosage rate of 10+5 viable organisms, as recommended for adult goats, should not be used on pregnant sheep.	1977	906093
properties of the berkeley phage lytic for "brucella melitensis" and other species.		1978	37809
cell-mediated immune response after brucella abortus s19 vaccination.	cell-mediated immunity to brucella abortus s19 vaccine was measured in young heifers by the microassay for stimulation of protein synthesis (sps) with [3h]leucine and the skin test for delayed hypersensitivity. brucella melitensis protein allergen and a crude b abortus s19-soluble antigen were compared in the sps test. the sps test was negative in 5 unvaccinated heifers and strongly positive in 3 twice-vaccinated steers. however, the sps test was positive only in 13 of 30 s19-vaccinated heifers ...	1978	103470
[induction of auxotrophy in the brucellosis microbe under the effect of n-methyl-n'-nitro-n-nitrosoguannidine].	mutagenic activity of n-methyl-n'-nitro-n-nitrosoguanidine with respect to brucella melitensis was studied. in the course of this study the method of obtaining auxotrophic brucella mutants was elaborated. numerous assortiment of auxotrophic mutants of different types was obtained. in a number of cases the acquisition of auxotrophy was accompanied by profound changes of the strain, such as the changes in morphology of colonies, in the biochemical activity, in the capacity of agglutination and in ...	1978	414962
successful treatment of brucella melitensis end-carditis.	brucella endocarditis is a rare, but often fatal, complication of brucellosis. a 32 year old man acquired brucellosis while on a visit to his former home in greece and presented six months later with malaise, fever and aortic regurgitation. blood cultures grew brucella melitensis biotype 1. combined chemotherapy with streptomycin, tetracycline and rifampin sterilized his blood; however, his aortic valve was replaced owing to recurrent emboli and cardiac failure. over the next 18 months the patie ...	1978	645754
serological response of adult goats infected with live brucella melitensis.		1978	719519
studies on the immune protection to murine experimental brucellosis conferred by brucella fractions. i. positive role of immune serum.	mouse inoculation with three different phenol-insoluble fractions extracted from brucella melitensis (fractions 'pi', '4a' and '5') induces an acceleration of the blood clearance of i.v. inoculated live brucella and a diminution of the rate of multiplication of the injected bacteria in the spleen. preincubation of the challenge inoculum in immune serum or i.p. injections of immune serum confer a good specific protection to non-immunized hosts. the results observed with fractionated sera suggest ...	1978	750371
brucella melitensis hepatitis: the absence of granulomas.		1979	475172
inflammatory response in the placental attachments of the sheep following experimental infection with brucella melitensis.		1979	544456
cell-mediated immune responses in cattle vaccinated with killed brucella melitensis strain h-38 vaccine or infected with viable brucella abortus strain 2308 organisms, or both.		1979	110181
studies on the genetic control of murine humoral response to immunization with a peptidoglycan-containing fraction extracted from brucella melitensis.	a peptidoglycan containing fraction (fraction "5") extracted from brucella melitensis has been injected in low infra-vaccinating doses into inbred mice. the genetic control of the resulting anti-brucella humoral response has been studied in the c57bl/6 "good responder" x dba2 "low responder" model. the results observed in f1, f2 and reciprocal backcrosses show that the "good responder" character, although transmitted as a dominant trait, is under polygenic control and independent of h2 haplotype ...	1979	118703
comparison of brucella abortus and brucella melitensis infections of mice and their effect on acquired cellular resistance.	by using mice infected with strains of brucella abortus and brucella melitensis we examined the histological responses to infection, the relationship of histology to persistence of organisms, and the relation of persistence of organisms to the acquisition of acquired cellular resistance (acr). infection with b. abortus resulted in well-formed granulomas in the livers, which persisted for more than 30 days. in contrast, infection with b. melitensis produced microabscesses in the livers which reso ...	1979	121113
granulomas in brucella melitensis infection.		1980	7352742
brucellosis treated with rifampicin.	14 children, aged between 15 months and 14 years, with brucellosis were treated with oral rifampicin only (20 mg/kg per day) for 3 weeks. laboratory diagnosis depended on blood culture (positive for brucella melitensis in 11 of the cases), serum agglutination, complement-fixation test, and coomb's test. response was good in each child, with fever clearing between the 2nd and 8th day. 2 children relapsed, but one relapse was probably a reinfection from contaminated cheese. both children were give ...	1980	7436494
[brucella melitensis endocarditis].		1980	7450582
bactericidal action of amoxycillin alone and in combination with gentamicin and rifampicin against brucella melitensis.		1980	7185916
brucella septicemia in pregnancy.	a case of brucella melitensis septicemia in a second-trimester pregnancy causing intrauterine fetal death and gram-negative septic shock with diffuse intravascular coagulation is reported. the literature is reviewed. this is the first reported case of human brucellosis in association with gram-negative sepsis and dic during pregnancy. the importance of blood cultures and agglutinins for brucella in febrile pregnant patients is re-emphasized.	1980	7189163
[histopathology of the hepatic lesions in brucellosis based on biopsy findings].	22 liver biopsies from patients affected by brucellosis (brucella melitensis) have been studied as far as granuloma formation is concerned. the first phase of granulomatous lesion seems to be the presence of monocytes within the hepatic sinusoids. then we can observe small histiocytic granuloma and finally a "mature" (adams [1]) granuloma, i.e. an organized collection of mature mononuclear phagocytes without necrosis, but sometimes with giant cells.	1980	7244086
experimental "brucella melitensis" infection in new zealand black (nzb) mice.		1980	7247501
comparison of four serological tests in the diagnosis of caprine brucellosis.	results from four serological tests for diagnosing brucellosis--serum tube agglutination (sat), agar gel immunodiffusion (agit), rose bengal plate (rbpt) and complement fixation (cft)--were compared using sera from goats from farms infected with brucella melitensis. ninety-two goats were negative and 29 positive to all four tests. the remaining 85 reacted to one or more tests. the rbpt was the most sensitive test and the agit the most specific, when compared with the cft. the results suggest tha ...	1980	6774391
enhancement of murine bone marrow colony formation and l-transformation by brucella antigen.	brucella melitensis and pseudomonas aeruginos antigens, in the form of heat-killed cells, enhanced serum stimulation of colony formation by mouse bone marrow progenitor cells. the antigens also enhanced colony formation in unstimulated medium. prior sensitization of the c57bl mice by recent infection or by immunization six months earlier increased sensitivity of bone marrow cells to brucella antigen enhancement of colony formation. the immunized mice provided marrow cells more primed to colony f ...	1980	6775795
isolation of three brucella abortus cell-wall antigens protective in murine experimental brucellosis.	the insoluble fraction, sds-i, obtained by boiling cell-walls of brucella abortus in sodium dodecyl sulfate (sds) was hydrolyzed by lysozyme. three major bands of apparent molecular weight 37000 (i), 25000 (ii), 15000 (iii) were isolated by gel slicing after polyacrylamide gel electrophoresis in the presence of sds. the three bands were injected subcutaneously to mice using freund incomplete adjuvant and immunity was tested one month later by intraperitoneal administration of a virulent strain o ...	1980	6802061
mitogenic activity of two brucella fractions for murine b lymphocytes.	two lipid a-free fractions extracted from brucella melitensis (fractions pi and sf) were shown to behave as b-cell non-specific mitogens for murine lymphocytes: they stimulated the uptake of tritiated thymidine by normal unsensitized murine splenic lymphocytes, by spleen cells from nude mice and by t-cell depleted but not by t-cell enriched and b-cell-deprived splenic populations. since depletion of adherent cells leads to a two- to three-fold depression of pi- or sf-induced mitogenic responses ...	1980	6968292
rapid identification of human b-lymphocytes and monocytes with rhodamine-labeled brucella melitensis.	the bacterium brucella melitensis has been shown to bind selectively to human b-lymphocytes. the specificity of this binding can be exploited in a rapid technique for the determination of human b-lymphocytes and monocytes by use of rhodamine-labeled brucella melitensis. this fluorescent labeled regent is simple to use and provides highly specific identification of human b-lymphocytes and monocytes, as demonstrated in a series of experiments characterizing known cellular markers on t- and b-lymph ...	1981	6972976
the use of salmonella schottmulleri for mapping and separation of human lymphocyte subpopulations.	human lymphocyte subpopulations (b cells, b1, b2, t1, t2, t3, and t4 cells; our denomination) have been previously identified and isolated by bacterial adherence and functional differences between them have been demonstrated. here we examined the binding properties of salmonella schottmulleri to human lymphocytes in peripheral blood smears and found that it binds to more lymphocyte subpopulations, namely b, t1, t2 and t3 cells, than any bacteria previously tested. thus, using only four bacteria: ...	1981	6976306
binding of bacteria from the genus brucella to human b lymphocytes.	in previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. among these bacteria was a strain of brucella melitensis which bound to all human b lymphocytes. to determine whether the binding of b. melitensis to human b lymphocytes was strain, species, or genus characteristic, we tested the binding of b. melitensis, brucella abortus, brucella ovis, brucella suis, brucella canis and brucella neotomae to human normal a ...	1981	6783546
[isolation of brucella melitensis from pleural fluid].		1981	7253750
causes of abortion in khorassan province--mashad.	the aim of this paper is to analyse the cause of abortion and postnatal deaths in sheep on the basis of laboratory examinations. for serological examination against brucellosis and salmonellosis 899 blood samples were collected. from all tested sera 411 (45.7%) were positive to brucella melitensis antigen and only 10 (1.11%) to salmonella abortus ovis antigens. bacteriological examination were done with 50 lambs, 143 aborted foetuses and 46 vaginal swabs. brucella melitensis was stated in 7.6% i ...	1981	7301632
protection of rams against brucella ovis infection by brucella melitensis rev. 1 vaccine.		1981	7324641
studies on brucella melitensis rev. 1 vaccine in goats.		1981	7342582
[brucella melitensis endocarditis. clinical and immunological features (author's transl)].	five cases of brucella melitensis endocarditis are reported. common features included subacute course, enhanced humoral immunity, and deficient cellular immunity. appropriate antibiotic therapy led to recovery in all cases. in two patients, valvular replacement was necessary.	1982	6277006
in vitro activity of n-formimidoyl thienamycin against 98 clinical isolates of brucella melitensis compared with those of cefoxitin, rifampin, tetracycline, and co-trimoxazole.	in vitro susceptibilities of 98 isolates of brucella melitensis to n-formimidoyl thienamycin, tetracycline, co-trimoxazole, rifampin, and cefoxitin were determined. n-formimidoyl thienamycin showed good activity which was similar to those of tetracycline and rifampin and different from that of the other beta-lactam antibiotic tested (cefoxitin), which showed poor activity. co-trimoxazole showed good activity.	1982	6980624
[enteral yersiniosis--a serious disease? current knowledge of clinical aspects, diagnosis and therapy].	enteral yersiniosis is caused either by y. enterocolitica 0-group i (syn. serotype 0:3) and 0-group v (syn. serotype 0:9) or y. pseudotuberculosis type i-vi. the clinical symptoms are mostly like enteritis, enterocolitis, acute abdomen, mesenteric lymphadenitis, or ileitis terminalis. post-infection reactions are possible like septicemia, arthritis and erythema nodosum. only cultural and serological examinations confirm the diagnosis of enteral yersiniosis. in the judgement of serological result ...	1982	7095694
the immune response of goats vaccinated with low and high doses of brucella melitensis rev 1.	the serological response, lymphocyte reactivity, and dermal hypersensitivity reactions of goats vaccinated with high and low doses of brucella melitensis rev 1 organisms to brucella antigens were studied. antibodies to soluble antigen a2 were detectable by immunoelectrophoresis (ie), appeared later than agglutinating antibodies but disappeared faster in most vaccinated animals. anti-a2 antibodies appeared earlier in goats which received the high dose. antibodies to polysaccharide b antigen were ...	1982	7112914
[development of brucella abortus, brucella melitensis and brucella and brucella suis infection infection in mice to a chronic stage and cure].		1982	7168538
efficacy of brucella melitensis rev. 1 vaccine against brucella ovis infection in rams.	the efficacy of brucella melitensis strain rev. 1 vaccine against a brucella ovis experimental infection was judged on 30 rams, 15 of which had received 8.5 x 10(8) rev. 1 at the age of 4 months. all were challenged 9 months later by conjunctival route with 5 x 10(8) b. ovis. the 30 rams were killed three months after challenge and were autopsied. genital and other organs and lymph-nodes were searched for b. ovis. only a few organs and lymph-nodes of three vaccinated rams shed b. ovis, contrary ...	1982	7168540
frequency of brucella melitensis in southern iran.		1982	7183780
comparison between subcutaneous and conjunctival route of vaccination with rev. 1 strain against brucella melitensis infection in ewes.	in areas where the prevalence of brucella melitensis infection in sheep is high, control of the disease cannot be led without the help of vaccination. the use of brucella melitensis strain rev. 1 vaccine affords a very good protection. the drawback is that this vaccine, giving rise to long-lasting antibody titers, may disturb serological screening when a return is made to a test and slaughter policy, after several years of vaccination. there is a great need for another mode of vaccination which ...	1982	7185320
pancytopenia due to hemophagocytosis in patients with brucellosis: a report of four cases.	in 42 patients with brucellosis who had bone-marrow cultures performed, five had hemophagocytosis, four of whom demonstrated peripheral hemocytopenia. all five patients with hemophagocytosis had brucella melitensis isolated from both blood and bone-marrow cultures. infectious diseases such as brucellosis should be considered in patients whose clinical picture suggests malignant histiocytosis, especially in geographic areas where brucellar infections are common.	1983	6833793
the presence of granulomas due to brucella melitensis in hepatitis.		1983	6833806
brucella melitensis: person-to-person transmission?		1983	6132121
induction of autoantibodies and circulating immune complexes in mice after injection of brucella fraction "pi" or inoculation with live brucella suis.	"pi", a vaccinating fraction with b mitogenic and polyclonal activator (pca) activity extracted from brucella melitensis, was i. p. injected into b6 and db mice. in b6 mice, this led to an important but transient increase in the level of anti-ssdna antibodies, the induction of rheumatoid factor (rf) and the appearance of circulating immune complexes (cic). in db mice, on the other hand, the only significant effect consisted of a slight and ephemeral peak of cic in sera from day 7. similarly, whi ...	1983	6602586
the use of allergic test in the diagnosis of brucella melitensis infection in sheep.		1983	6626935
[stimulating action of brucella melitensis lipopolysaccharide on hemopoiesis in mice].	the bacterial mass, brucellar protective antigen and lipopolysaccharide (lps) obtained from b. melitensis stimulated the formation of endogenous colonies in the spleen of mice belonging to different strains, subjected to irradiation in sublethal doses. the maximum stimulating effect was observed when the antigens were introduced 24 hours prior to irradiation. lps introduced in the optimal dose induced an increase in the number of hemopoietic stem cells (hsc) in the s-phase of the cell cycle, thu ...	1983	6637272
serological response of sheep to brucella melitensis rev. 1 vaccine.		1983	6650024
brucellosis in riyadh, saudi arabia. a microbiological and clinical study.	during a period of two years, 30 cases of brucellosis were positively diagnosed from a total of 209 patients who reported with prolonged fever for investigation. diagnosis was made both by blood culture and serological tests. the latter included slide and tube agglutination in all cases and an enzyme linked immunosorbent assay (elisa) in 16. 11 cases (36.7%) gave negative results by the slide-agglutination screening test used at the recommended single serum dilution of 1:80. this was due to the ...	1983	6665836
brucellosis: difficulties in diagnosis and a report on 38 cases.	we have reviewed 38 patients with brucellosis who were admitted to the chaim sheba medical center between 1955 and 1977, i.e. during a 23-year period. the clinical manifestations varied greatly. diagnosis was easy when brucellosis was suspected, as in cases presenting with fever of unknown origin. in nine of the 38 cases diagnosis was difficult and delayed for several weeks since brucellosis was not suspected. some of these cases will be described in detail. the diagnosis was established in 16 p ...	1983	6668070
brucellosis in spouses and the possibility of interhuman infection.	this paper deals with a brucella infection in a laboratory worker and his wife. the diagnosis of brucellosis was proved by isolating brucella melitensis, biotype 2, from blood cultures and by the standard serum agglutination test. in the absence of any alternative epidemiologic information, the authors consider this case to be an example of interhuman infection.	1983	6668071
elevated risk of osteoarticular complications in children with acute brucella melitensis infection.	infection with brucella microorganisms is considered uncommon in the paediatric age group. we report nine paediatric patients between the ages of 8 to 17 years with acute brucella melitensis infection, who presented with spiking fever, night sweats, anorexia and malaise for 5 to 60 days prior to diagnosis. four patients developed various osteoarticular complications: migratory arthralgia, hydroarthrosis of the knees, arthritis and osteomyelitis. therapy with tetracyclines alone or in combination ...	1983	6671069
serological response of cattle, sheep and goats in kenya vaccinated with killed brucella melitensis strain h38 adjuvant vaccine.	the antibody responses of cattle, sheep and goats to the brucella melitensis h38 adjuvant vaccine were monitored by serum (tube) agglutination, complement fixation and rose bengal plate tests. high and persisting antibody titres were induced by a single dose of this vaccine in all three species. it would be difficult to classify reactors by commonly used diagnostic tests in a control or an eradication programme using the h38 adjuvant vaccine.	1983	6346660
the use of mutants of escherichia coli for the identification of human lymphocyte subpopulations in blood smears.	in previous work we have shown that some bacteria can bind to human lymphocytes and can be used to identify lymphocyte subpopulations in conventionally stained blood smears. these bacteria are of different species or genera, which makes it difficult to study the binding mechanism. also, the main marker for b cells, brucella melitensis, is of very small size and highly pathogenic. here we show that b cells as well as some of the t cell subpopulations can be identified by different mutants obtaine ...	1983	6350450
[identification and taxonomy of brucella melitensis by factor analysis of the relations and automatic classification].		1984	6386576
[discrimination of taxa of the species brucella melitensis and the determination of strain types].		1984	6386577
comparison of phenol- and heat-killed antigens in the indirect immunofluorescence test for serodiagnosis of legionella pneumophila group 1 infections.	an antigen prepared with agar-grown legionella pneumophila group 1 killed by 0.5% phenol and suspended in 0.5% yolk sac was examined for use in the indirect immunofluorescence test for legionellosis and compared with a heat-killed antigen. the serological results of the two antigens for single and paired sera agreed well. morphological and staining characteristics were better for phenol-treated organisms. electron microscopy observation showed an apparently well-preserved cell surface. the backg ...	1984	6386881
immunochemical characterization of rough brucella lipopolysaccharides.	lipopolysaccharides (lps) were extracted from rough strains of brucella abortus and brucella melitensis and from strains of the naturally occurring rough species brucella ovis and brucella canis. brucella rough lipopolysaccharides (r-lps) were readily distinguished from brucella smooth lipopolysaccharides (s-lps) and enterobacterial r-lps, by their chemical, physical, and serological characteristics. b. ovis r-lps was differentiated from b. abortus, b. melitensis, and b. canis r-lps by its react ...	1984	6421737
evaluation of a radial immunodiffusion test for diagnosing brucellosis in sheep and its possible value for differentiating infected from brucella melitensis rev 1 vaccinated sheep.	the efficiency of 4 serological test: rose bengal (rb), complement fixation (cf), gel diffusion (gd) and radial immunodiffusion (rid) for diagnosing brucellosis in sheep was compared. rid and cf were also used to evaluate sheep serological response following rev 1 vaccination. crude smooth lipopolysaccharide (lps-s) and polysaccharide b antigens obtained from b. melitensis 16 m were used in gd and rid tests respectively. in experiment 1, two hundred and sixty five sera from adult unvaccinated sh ...	1984	6436109
brucellosis in a farming community in saudi arabia.	this communication describes a study of the prevalence of brucellosis in a farming community of saudi arabia. standard agglutination tests of sera from 21 patients were tested for brucella abortus and brucella melitensis. the results show that 8 (38%) were positive with titres of 160 or over 20 480 and with titre of complement fixation tests ranging from 8 to 64. agglutination titres of 20 to 40 were present in only 3 cases. those patients with positive agglutination titre of 160 or higher admit ...	1984	6436116
lymphocyte bacterial rosette test: methodological details and effect of metabolic inhibitors and divalent cations.	lymphocytes bind certain bacteria. this property has been utilized in the lymphocyte bacterial rosette assay to identify t and b cell subsets. here we determined the optimum conditions for the assay, studied the effect of metabolic inhibitors and divalent cations and compared bacterial rosette-forming lymphocyte subpopulations with those defined with monoclonal antibodies. the strains used were brucella melitensis, bacillus subtilis, staphylococcus aureus and escherichia coli. optimum attachment ...	1984	6440915
in vitro activity of ciprofloxacin against brucella melitensis.		1984	6489329