Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| bacterial numbers on penicylinders used in disinfectant testing: use of 24 hour adjusted broth cultures. | the current aoac use-dilution methods of disinfectant efficacy testing require the use of 48-54 h unadjusted broth cultures of salmonella choleraesuis, staphylococcus aureus, and pseudomonas aeruginosa for the inoculation of stainless steel penicylinders. the use of unadjusted broth cultures contributes to noncomparable numbers of organisms on penicylinders among the test strains due to relative efficacy of bacterial attachment to penicylinders and to bacterial numbers in broth. to achieve compa ... | 1988 | 3129396 |
| comparison of the invasion strategies used by salmonella cholerae-suis, shigella flexneri and yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication. | strains of escherichia, salmonella, shigella and yersinia actively enter eukaryotic cells. several techniques were used to compare and contrast the invasion mechanisms of salmonella cholerae-suis, yersinia enterocolitica and shigella flexneri. three animal cell lines (cho, hep-2 and mdck) were examined for susceptibility to bacterial entry by these strains. levels of intracellular bacteria varied widely between cell lines, but cho cells were the most susceptible to bacterial invasion, hep-2 inva ... | 1988 | 3147700 |
| quantitative evaluation of bacteria washed from stainless steel penicylinders during aoac use-dilution method. | stainless steel penicylinders inoculated separately with test bacteria (salmonella choleraesuis, pseudomonas aeruginosa, or staphylococcus aureus) are used in the aoac use-dilution method (udm) for disinfectant efficacy testing. numbers of bacteria remaining on penicylinders were quantitatively assessed to determine if cells are washed from the penicylinders after a 10 min exposure to phosphate buffer dilution water (pbdw). inoculated penicylinders were also examined by scanning electron microsc ... | 1988 | 3148609 |
| disinfectant testing using a modified use-dilution method: collaborative study. | an initial collaborative study of the aoac use-dilution method (udm), used for bactericidal disinfectant efficacy testing, demonstrated extreme variability of test results among the 18 laboratories testing identical hospital disinfectants. in an effort to improve the method, 32 changes were made by the udm task force. these changes represented improvements in quality assurance practices and elimination of method variability; however, the basic framework of the method was retained. a second colla ... | 1988 | 3149273 |
| occurrence of h-antigen z66 of r phase in cultures of salmonella serovar typhi originated from indonesia. | eighteen strains of salmonella choleraesuis subsp. choleraesuis serovar typhi (s. typhi) isolated from blood of patients in japan who had visited indonesia and returned before the onset of typhoid fever were found to possess the h-antigen z66 reported by guinée et al. (1981) but had the naturally occurring h-antigen d. they were not lysed by any of the phages of the vi phage typing system. after passage through semi-solid medium containing h-z66 antiserum, h-antigens of 11 of 18 cultures with h- ... | 1988 | 3181315 |
| quantitative analysis and partial characterization of cytotoxin production by salmonella strains. | the pathogenesis of the wide-spectrum human disease caused by salmonella species is poorly understood. cytotoxin production by other enteric pathogens has been increasingly investigated recently, and data are accumulating regarding the role of cytotoxins in enteric infections and hemolytic uremic syndrome. we studied the cytotoxic activity of 131 salmonella strains of the major serotypes, including 94 strains of salmonella enteritidis, 12 strains of salmonella typhi, and 25 strains of salmonella ... | 1988 | 3182072 |
| evidence of correlation between 50-kilobase plasmid of salmonella choleraesuis and its virulence. | a plasmid of 50 kilobases (kb) was found in all six strains of salmonella choleraesuis studied, originally isolated from septicemic swine or man. a derivative cured of the 50 kb plasmid was isolated after treatment with novobiocin, and its virulence compared to that of the parent by intraperitoneal injection into mice. the lethality of the parent strain was found higher than that of the cured strain. with a sublethal number of cells of the parent strain injected, the spleen, liver and lymph node ... | 1988 | 3200162 |
| clinical safety and efficacy of ofloxacin. | in this study we report about the efficacy and tolerability of ofloxacin in the treatment of 15 patients with severe and moderately severe infections including osteomyelitis (5), soft tissue infections (5), salmonellosis in aids patients (2), acute or chronic pulmonary infections (2) and mediastinitis (1). the following organisms were isolated in culture specimens: staphylococcus aureus (4), pseudomonas aeruginosa (4), staphylococcus epidermidis (3), serratia marcescens (1), escherichia coli (1) ... | 1988 | 3224401 |
| abscess thyroid due to salmonella cholerae-suis--a rare presentation of salmonellosis. | 1988 | 3229806 | |
| [the aerobic intestinal flora of the microchiropteran bat chaerephon pumila in madagascar]. | from stools of 88 malagasy insect eater bats chaerephon pumila, a large amount of bacterial strains were isolated. twenty different species were recognized, most of them belonged to enterobacteriaceae family. eight strains were identified as salmonella enterica subsp. enterica o48: -; 1.5, despite many atypical characters. strains of salmonella enteritidis, koserella trabulsii, kluyvera sp., odc negative serratia marcescens, atypical hafnia alvei... were also identified. | 1988 | 3240566 |
| [acute bacterial sacroiliitis caused by salmonella cholerae-suis]. | a 17-year-old schoolboy was admitted to hospital because of one-sided pelvic pain of uncertain aetiology and fever gradually rising over several days. bacteriological analysis of blood cultures, skeletal scintigraphy and computed tomography revealed sacroiliitis caused by salmonella cholerae-suis. specific antibiotic treatment quickly stopped all symptoms and cured the infection. radiologically there remained sclerosis of the sacro-iliac joint. | 1988 | 3262497 |
| penetration of salmonella through a polarized madin-darby canine kidney epithelial cell monolayer. | many intracellular parasites are capable of penetrating host epithelial barriers. to study this process in more detail we examined the interactions between the pathogenic bacteria salmonella choleraesuis and polarized epithelial monolayers of madin-darby canine kidney (mdck) cells grown on membrane filters. association of bacteria with the mdck cell apical surface was an active event, requiring bacterial rna and protein synthesis, and was blocked by low temperatures. salmonella were internalized ... | 1988 | 3292541 |
| extraintestinal salmonellosis. | between 1969 and 1984, 6564 non-typhoid salmonella strains were isolated at the liverpool public health laboratory of which 194 (3.0%) were from extraintestinal sites. blood (34%) and urine (32%) isolates accounted for two-thirds of these, with the remainder being recovered from pus and inflammatory tissue (23%), bone (5%), cerebrospinal fluid (5%) and sputum (3%). certain serotypes tended to cause more invasive disease than others, i.e. salmonella choleraesuis, s. dublin, s. london, s. virchow ... | 1988 | 3378582 |
| clonal nature of salmonella typhi and its genetic relatedness to other salmonellae as shown by multilocus enzyme electrophoresis, and proposal of salmonella bongori comb. nov. | crude cell extracts of 26 isolates of salmonella serotype typhi (s. typhi) and 48 other salmonella isolates representing 28 serotypes and seven dna hybridization subgroups were analyzed for electrophoretic variants of 24 metabolic enzymes by starch gel electrophoresis. all strains of s. typhi had identical isoenzyme patterns, indicating that they were a single clone. all of the enzymes detected in the remaining strains were polymorphic, and the degree of genetic variation was quite high. the ave ... | 1989 | 2915026 |
| [multicenter study on bacterial gastroenteritis in regional hospitals of the province of barcelona. group of microbiologists of the regional hospitals of catalonia]. | a retrospective analysis of 26,901 coprocultures is reported. of these, in 5,847 (21.7%) an enteric pathogen was isolated. the positivity rate in adults was 21.0%, and 22.1% in children. statistically significant differences (p less than 0.001) were found in the number of overall isolates throughout the years for both age groups. the most common enteropathogen was salmonella enterica spp i non typhi (59.4% of the isolates in children and 76.9% in adults). the rate of isolation of campylobacter s ... | 1989 | 2490674 |
| [phenolic coefficient in the microbiological evaluation of disinfectants for hospital and household use]. | the phenolic coefficient of 24 disinfectants (six for hospital and the remainder for household use) commercialized in s. paulo were verified. the active compounds found were phenol, quaternary ammonium, formaldehyde, ethanol and chlorine, some of them in association. the microorganisms used were staphylococcus aureus atcc 6538, pseudomonas aeruginosa atcc 15442 and salmonella choleraesuis atcc 10708. the values of the phenolic coefficient ranged from 58.3 to 0.1. the hospital disinfectants showe ... | 1989 | 2515584 |
| molecular cloning and analysis of the incompatibility and partition functions of the virulence plasmid of salmonella typhimurium. | the incompatibility functions (inc) of the virulence plasmid of salmonella typhimurium lt2 were initially located in a 4.3 kb region near the repa locus of the plasmid. expression of inc required the presence, in cis or in trans, of two distinct dna regions of the fragment. these regions, maximally 0.3 kb and 0.6 kb in size, were separated in the fragment by c. 3.0 kb. this intervening dna encoded two proteins, of mr values 37 kda and 40 kda. the promoter for the 37 kda protein lay in or near on ... | 1989 | 2556621 |
| porcine neutrophil function in the presence of virulent and avirulent salmonella choleraesuis. | porcine polymorphonuclear leukocytes (pmnls) may be activated by bacteria to begin phagocytosis followed by oxidative and non-oxidative mechanisms of killing. the purpose of this study was to identify differences between virulent and avirulent salmonella choleraesuis (s. choleraesuis) strains, 38 and 9 respectively, in their interactions with porcine pmnls using five different assays. (1) staphylococcus aureus (s. aureus) ingestion was determined by exposure of porcine pmnls to a mixture of s. c ... | 1989 | 2560864 |
| vaccination route, infectivity and thioglycollate broth administration: effects on live vaccine efficacy of auxotrophic derivatives of salmonella choleraesuis. | an aromatic-dependent, therefore non-virulent, derivative of a mouse-virulent strain of salmonella choleraesuis previously shown not to be effective as a live vaccine when given intraperitoneally (i.p.) to itys mice, was administered to balb/c mice. two doses given i.p. or by feeding did not protect against i.p. or oral challenge with 50 to 5000 ld50 of the virulent ancestor strain. by contrast two doses given intravenously (i.v.) gave almost complete protection against i.p. or oral challenge wi ... | 1989 | 2622327 |
| prevalence of salmonella serotypes isolated in spain from human and non human sources (1983-1987). | salmonella serotypes over a five year period were studied in order to know their prevalence in spain. the salmonella reference centre received a total of 17,612 strains from 1983-1987. the majority (16,133) were of human origin and only 1,479 strains were isolated from non-human sources. the serotyping yielded 100 different serotypes, salmonella enterica serotype enteritidis (8) being the commonest in both groups, 61.18% of human origin and 31.91% of non-human origin. salmonella enterica serotyp ... | 1989 | 2629788 |
| long-term suppression of salmonella aortitis with an oral antibiotic. | an aneurysm of the aortic arch infected with salmonella choleraesuis is presumed to be inevitably fatal unless the patient receives corrective surgery and antibiotic therapy. i report such a case in a patient who refused surgery and who has been maintained free of infectious symptoms for over 26 months using trimethoprim-sulfamethoxazole administered orally. | 1989 | 2786398 |
| in vitro activity of tigemonam against urinary tract pathogens. | tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serratia marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory ... | 1989 | 2809699 |
| in vitro activity of tigemonam against urinary tract pathogens. | tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serrada marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory c ... | 1989 | 27416146 |
| rrna probing of chromosomal dna of epidemic and sporadic isolates of salmonella enterica subsp. enterica serovar kottbus from northern and southern italy. | fifty-two strains of salmonella enterica subsp. enterica serovar kottbus, identified at the centres of enterobacteriaceae of northern and southern italy, were investigated by molecular genetic methods. thirteen isolates were recovered during two food-poisoning outbreaks that occurred in may 1987 in lombardy. the rdna gene restriction patterns, obtained by probing endonuclease cleaved chromosomal dna with photobiotin labeled escherichia coli rrna, revealed some heterogeneity among strains isolate ... | 1990 | 2091942 |
| [salmonella enterica in the avilés health district (asturias). microbiological, clinical, and epidemiological aspects]. | the results of the follow up of the salmonella enterica infections seen in the hospital san agustin in avilés (asturias) from 1984 through 1987 are reported. the incidence was 19.49% of the overall number of fecal cultures and 0.47% of the overall number of blood cultures. 326 patients were involved: acute gastroenteritis (314 cases, with bacteremia in 10), typhoid fever (7 cases) and other infections (5 cases); 172 patients required hospital attention and 115 antimicrobial therapy. the morbidit ... | 1990 | 2094387 |
| experimental ovine salmonellosis (salmonella abortusovis): pathogenesis and vaccination. | salmonella enterica subsp. enterica ser. abortusovis, a sheep-adapted serotype, causes a contagious disease. abortion is the major symptom and the main source of contamination. research on this ovine disease may aid farmers, but may also contribute to comparative biological knowledge. innate resistance partly controlled by the ity locus, increased resistance to reinfection and humoral and t-cell-mediated immunity were observations gained with a murine model. in ewes, abortion regularly occurs fo ... | 1990 | 2101486 |
| [microbiological evaluation of chemical disinfectants for household use]. | five disinfectants for household use were advertised on television during 1988 and the first half of 1989. the products were tested by a qualitative (use-dilution with 10 carriers, a conventional and simplified, adapted method) and a qualitative, adapted method with a view to evaluating their antimicrobial activity. the active compounds of the products, according to their respective labels were: 1--parachlorophenol (0-benzil) 0.1%; 2--eter 2.4.4' chloro (iii) 2' hydroxiphenylic 0.1%; 3--n-alkyl ... | 1990 | 2120768 |
| [etiological research on acute gastroenteritis in the city of cádiz]. | we researched the microbial etiology of 5,836 diarrheal processes in outpatients and inpatients at the social security hospital in cádiz, for the purpose of evaluating their microbiological and epidemiological characteristics. bacterial gastroenteritis accounted for 51.6% of the cases, while 31.7% were viral and 16.6% were protozoal in origin. salmonella enterica (35.6%) is the most frequently involved microorganism, especially in the summer, followed very closely by rotavirus (31.7%). the incid ... | 1990 | 2129735 |
| mice vaccinated with a non-virulent, aromatic-dependent mutant of salmonella choleraesuis die from challenge with its virulent parent but survive challenge with salmonella typhimurium. | balb/c mice given a live vaccine of an aroa mutant of salmonella choleraesuis by intraperitoneal (i.p.) injection were not protected against i.p. challenge with its virulent parental strain but were protected against i.p. challenge with either of two virulent strains of salmonella typhimurium (o [1], 4, [5], 12). vaccination with a live vaccine of s. typhimurium aroa protected against challenge with s. typhimurium but not with s. choleraesuis. intraperitoneal administration of either aroa strain ... | 1990 | 2139129 |
| [sensitivity of enteropathogenic bacteria to furazolidone]. | the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ... | 1990 | 2152485 |
| identification and mapping of mba regions of the salmonella choleraesuis virulence plasmid pkdsc50 responsible for mouse bacteremia. | a restriction cleavage map was constructed for pkdsc50, the 50 kb virulence plasmid of salmonella choleraesuis. using tn1 transposon-insertion mutagenesis, we obtained 19 mutant strains of s. choleraesuis with a diminished ability to cause bacteremia in the mouse. pkdsc50::tn1 dna of the mutant strains was extracted and introduced into a pkdsc50-cured s. choleraesuis strain. nine of the 19 transformants showed the diminished ability to cause mouse bacteremia. tn1-insertion of the nine mutant str ... | 1990 | 2159103 |
| peroxidase-antiperoxidase and immunogold labeling of salmonella typhimurium and salmonella choleraesuis var kunzendorf in tissues of experimentally infected swine. | peroxidase-antiperoxidase immunoenzymatic labeling and immunogold labeling techniques were evaluated for microscopic detection and location of salmonella organisms in tissues of experimentally infected swine. salmonella typhimurium and salmonella choleraesuis var kunzendorf were labeled specifically by the peroxidase-antiperoxidase technique in paraffin-embedded tissues of infected swine for conventional light microscopy and by postembedding immunogold labeling on ultrathin sections for electron ... | 1990 | 2183652 |
| plasmid profiles as an epidemiological marker for salmonella enterica serotype enteritidis foodborne outbreaks. | the incidence of enteritidis serotype of salmonella enterica in salmonellae infections has steadily increased in spain from 27.1% in 1982 up to 63.4% in 1987. given this high incidence, we have studied the plasmid profiles of enteritidis isolates to subclassify them. different profiles were observed in 50 isolates. in 13 enteritidis serotype outbreaks, up to 5 different plasmid profiles were found. each outbreak correlated with a single plasmid profile except in one case where plasmids of two di ... | 1990 | 2206470 |
| salmonella interactions with polarized human intestinal caco-2 epithelial cells. | polarized monolayers of the human intestinal epithelial caco-2 cell line were grown on permeable filters and infected apically with either salmonella choleraesuis or salmonella typhimurium. both salmonella species penetrated through the monolayer, requiring 2 h before appearing in the basolateral medium. both species caused a loss in transepithelial resistance by 3-4 h, and the monolayer's integrity was completely disrupted by 6 h. scanning and transmission electron microscopy revealed that the ... | 1990 | 2230236 |
| [epidemiology and clinical evaluation of salmonella enteritis]. | there was a local epidemic of salmonella enteritis in southern part of taiwan during the summer of 1989. from july through september 1989, a total 162 cases of enteritis were analysed in chang gung memorial hospital, kaohsiung. among them, 46 cases were proved to be salmonella enteritis by stool and/or blood culture. the identified flora group mainly group b (salmonella typhimurium, 87%), group c (salmonella choleraesuis, 6.5%) and group d (salmonella enteritidis, 6.5%). the drug resistance of s ... | 1990 | 2282566 |
| nucleotide sequence of a gene encoding a 29 kda polypeptide in mba region of the virulence plasmid, pkdsc50, of salmonella choleraesuis. | 1990 | 2315022 | |
| nucleotide sequences of genes encoding 32 kda and 70 kda polypeptides in mba region of the virulence plasmid, pkdsc50, of salmonella choleraesuis. | 1990 | 2336400 | |
| vaccinal properties of salmonella abortusovis mutants for streptomycin: screening with an ovine model. | living attenuated vaccines may help control infection with salmonella enterica subspecies enterica ser abortusovis in sheep without constraints incompatible with husbandry. virulence and immunogenicity of 2 abortusovis streptomycin-dependent strains and 3 reverse mutants were compared in sheep, the virulent parental strain abortusovis 15/5 being used as reference. reduction of virulence with conservation of some immunogenicity was demonstrated for the 5 mutant strains after intravenous or subcut ... | 1990 | 2344139 |
| alternative methods of attenuating salmonella species for potential vaccine use. | because of their invasive properties, salmonella species are uniquely suited for use as live oral vaccines, and mutants attenuated in general metabolic functions show promise as vaccine strains. we have identified a salmonella choleraesuis transposon mutant which is deficient in abilities to attach to, invade or penetrate through animal cells. this mutant is avirulent in mice, yet is able to persist within the liver, spleen and gut-associated lymphoid tissue for periods which may be adequate for ... | 1990 | 1966254 |
| characterization of strains of salmonella enterica subsp. enterica serovar wien isolated in italy: an epidemiological evaluation. | in the years 1987-1988, 110 strains of wien serovar were isolated from a gastroenteritis outbreak in a neonatal unit in palermo (sicily). these strains showed different drug resistance patterns and plasmid profiles. analysis of endonuclease restriction fragments of chromosomal dna by hybridization with e. coli rrna has demonstrated that a single bacterial clone or its derivatives were responsible for the outbreak. furthermore, the study of 139 strains, isolated since 1970 from different geograph ... | 1990 | 1982334 |
| [the characteristics of the o-specific humoral immune response of mice vaccinated with salmonella choleraesuis antigens]. | the work deals with the results of the comparative enzyme immunoassay (eia) of serum samples taken from (cba x c57bl/6) f1 mice immunized with o-specific polysaccharides, o-antigens (o-ag) obtained by boivin's method and antigenic preparations isolated with hydroxylamine (ha) from s. choleraesuis and s. typhimurium. o-ag and lipopolysaccharide (lps) of the corresponding bacterial species were used as antigens for the sensitization of polystyrene plates. the primary and secondary humoral immune r ... | 1990 | 1711262 |
| salmonella choleraesuis strains deficient in o antigen remain fully virulent for mice by parenteral inoculation but are avirulent by oral administration. | o-antigen-deficient derivatives of two mouse-virulent strains of salmonella choleraesuis (serogroup c1; o-6,7) were constructed by transduction of a long deletion of the rfb operon. strains sn36 and sn57 were derived from the smooth ancestor sl2824, while sn37 was derived from the smooth ancestor sl2840. these rfb deletion derivatives (rfb strains) had typical bacteriophage sensitivity patterns of "rough" salmonella strains and were at least 200,000 times more sensitive to serum than their smoot ... | 1990 | 1695204 |
| antigenic relationship of hanseniaspora and kloeckera with escherichia coli (o: 75, o: 163), salmonella cholerae-suis (o: 6(2), 7), salmonella aberdeen (o: 11) o antigens. | 1990 | 1698062 | |
| the antigenic relationship between salmonella cholerae suis (211) and salmonella aberdeen, escherichia coli (0 75, 0 163) o antigens. brief report. | 1990 | 1699559 | |
| lysogenization of salmonella choleraesuis by phage 14 increases average length of o-antigen chains, serum resistance and intraperitoneal mouse virulence. | three clones from a strain of salmonella choleraesuis (serogroup c1) were lysogenized with phage 14 (p14) which converts the o-antigen of serogroup c1 salmonellae from o-6,7 to o-6,7,14. the lysogens were compared with their parental non-lysogenic clones with respect to the following properties: average length of o-antigen polysaccharide chains, sensitivity to normal human serum, and mouse-virulence. sds-polyacrylamide gel electrophoresis of lipopolysaccharides extracted from these bacteria show ... | 1990 | 1702502 |
| properties of a rat monoclonal antibody reactive with both the mannan of candida species and the o-antigen 6,7 polysaccharide of serogroup c1 salmonellae. | monoclonal antibody masc1-mr9 of isotype immunoglobulin m was generated in lou/c rats by immunization with heat-killed salmonella thompson (serogroup c1, o-antigen 6,7 lipopolysaccharide) bacteria which had been further enriched for o-antigen by coating with homologous lipopolysaccharide. eight monoclonal antibodies were selected by screening against the lipopolysaccharide of s. thompson. in a subsequent test against candida mannan, only one antibody, masc1-mr9, was reactive. immunofluorescence ... | 1991 | 1702760 |
| plasmid profiles of salmonella enterica serovar berta isolated from broilers in denmark. | salmonella enterica serovar berta was isolated for the first time in denmark in october 1984. this serovar has subsequently become endemic in the broiler industry. plasmid profile analysis of 610 strains of s. berta isolated from broilers and broiler parent stock in the period october 1984 to july 1989 showed that plasmids were present in 85.7% of strains investigated, with a mean of 1.8 plasmids per strain. a significant increase in the mean number of plasmids per strain was seen during the obs ... | 1991 | 1648932 |
| presumptive identification of salmonella enterica using two rapid tests. | two rapid test for early detection of salmonella enterica, the cytochrome oxidase test and a fluorescence test, were evaluated in 1,200 colonies which had been isolated from human feces and cultured on macconkey and salmonella-shigella media. using the fluorescence test there were no false negative results (sensitivity 100%) and of 205 positive cases 62 did not correspond biochemically to salmonella (specificity 94.1%); 44 of these 62 were positive in the cytochrome oxidase test, raising the spe ... | 1991 | 1660809 |
| prevention of travellers' diarrhoea by oral b-subunit/whole-cell cholera vaccine. | b-subunit/whole-cell cholera vaccine (bs-wc) has been shown to give bangladeshi mothers and children only 3 months' protection against severe diarrhoea due to enterotoxigenic escherichia coli (etec). since a long-lasting effect is not necessary for protection against travellers' diarrhoea, a prospective double-blind study was conducted among tourists who went to morocco from finland. 307 tourists received two oral doses of bs-wc, whereas 308 controls received a placebo before departure. a resear ... | 1991 | 1682684 |
| the cps gene cluster of salmonella strain lt2 includes a second mannose pathway: sequence of two genes and relationship to genes in the rfb gene cluster. | we report the presence in salmonella enterica strain lt2 (serovar thyphimurium) of duplicate genes for two steps in the synthesis of gdp-mannose. the previously known genes, rfbk (phosphomannomutase) and rfbm (mannose-1-phosphate guanyltransferase), are part of the gene cluster for the o antigen. the two new genes, cpsb and cpsg, respectively, are thought to be part of the gene cluster for the m antigen capsular polysaccharide, present in many enterobacteriaceae. the two genes have been sequence ... | 1991 | 1712067 |
| lack of the alpha-1,2-linked n-acetyl-d-glucosamine epitope in the outer core structures of lipopolysaccharides from certain o serogroups and subspecies of salmonella enterica. | a total of 176 strains of salmonella enterica representing 116 serotypes were tested for the presence of the t6 epitope of the alpha-1,2-linked n-acetyl-d-glucosamine residue by reaction with a murine monoclonal antibody t6 specific for this structure in the salmonella ra core lipopolysaccharide (lps). all 20 serotypes (70 strains) belonging to serogroups a to e were positive for the t6 epitope while 29% of the 96 serotypes (106 strains) belonging to o serogroups f to 67 were negative; 12 seroty ... | 1991 | 1719590 |
| molecular cloning and expression in escherichia coli k-12 of the rfb gene cluster determining the o antigen of an e. coli o111 strain. | the o antigen of escherichia coli o111 is identical in structure to that of salmonella enterica serovar adelaide. another o-antigen structure, similar to that of e. coli o111 and s. enterica serovar adelaide is found in both e. coli o55 and s. enterica serovar greenside. both o-antigen structures contain colitose, a 3,6 dideoxyhexose found only rarely in the enterobacteriaceae. the o-antigen structure is determined by genes generally located in the rfb gene cluster. we cloned the rfb gene cluste ... | 1991 | 1722559 |
| molecular cloning and genetic characterization of the rfb region from yersinia pseudotuberculosis serogroup iia, which determines the formation of the 3,6-dideoxyhexose abequose. | the rfb region of yersinia pseudotuberculosis serogroup iia has been cloned and expression of o antigen in escherichia coli k12 was demonstrated. transposon mutagenesis analysis confined the dna region required for o antigen expression to a 19.3 kb fragment, and the o antigen expressed was visualized by sds-page and silver staining. southern hybridization analysis demonstrated significant levels of similarity between the yersinia rfb region and the 3,6-dideoxyhexose pathway genes rfbf and rfbg, ... | 1991 | 1724263 |
| epidemiological evaluation by rrna-dna hybridization of strains of salmonella enterica subsp. enterica serovar abortusovis isolated in southern italy in the years 1981-1989. | salmonella enterica subsp. enterica serovar abortusovis is a major agent of abortion of the sheep and is firmly established, although at low prevalence, in sicily. this paper describes the application rdna gene restriction pattern fingerprinting to investigate relatedness among 7 serovar abortusovis strains isolated at the "istituto zooprofilattico sperimentale" of sicily and 29 isolates identified at the southern italy centre of enterobacteriaceae between 1981 and 1989. although abortusovis ser ... | 1991 | 1727022 |
| a pathological study on the virulence of salmonella choleraesuis associated with 50-kilobase plasmid in mice. | the virulence of salmonella choleraesuis associated with 50-kilobase plasmid was assessed by the pathology produced in mice. the strains used in this study were plasmid-contained rf-1, plasmid-cured 31n-1 and plasmid-reintroduced 31n-1-t. mice were intraperitoneally inoculated with sublethal doses of these bacteria and chronologically necropsied. pathologically, the plasmid-positive (p+) cases were distinctly different from the plasmid-negative (p-) ones, especially in the peritoneal fluids and ... | 1991 | 2015199 |
| [increase in ampicillin resistance in salmonella enterica]. | 1991 | 2023473 | |
| human infection with salmonella dublin. | twenty-seven cases of human infection with salmonella dublin were identified over a 12-year period at the university of california at san diego-affiliated hospitals. important epidemiologic risk factors were the ingestion of unpasteurized dairy products or treatment with nutritional therapy that included raw calf-liver extracts. nearly all patients had underlying chronic diseases. like salmonella choleraesuis, s. dublin infections were associated with a high incidence of bacteremia (91%), metast ... | 1991 | 2030643 |
| an unusual presentation of salmonellal aortitis. | our patient represents what we believe to be the first documented case of salmonella choleraesuis aortitis presenting as a salmonellal empyema in an elderly diabetic man. although s choleraesuis often causes septicemia, its absence should not lead one to forget the pathogenic nature of and high mortality associated with this organism. aggressive efforts must be made to search for endovascular infection, because cures can be achieved only with surgical intervention and prolonged antibiotic therap ... | 1991 | 2035095 |
| abortion and serological reaction of ewes after conjunctival instillation of salmonella enterica subsp enterica ser abortusovis. | the aim of the experiment was to evaluate in pregnant ewes the clinical, bacteriological and serological effects of conjunctival instillation of s abortusovis. an individual dose of 1 x 10(10) viable s abortusovis were administered to 20 ewes 92 d after mating. among 18 pregnant ewes, 8 aborted 110 +/- 13 d after mating, 4 had stillborn lambs and 6 a clinically normal lambing (114 +/- 1.3 d). s abortusovis was isolated from placentas, swabs and foetuses of the 12 lambings. evolution of "h" agglu ... | 1991 | 2042907 |
| [bacterial diarrhea in infancy: epidemiologic study of 256 cases]. | infectious diarrhoea are common causes of morbility in children. although viral agents are responsible for the majority of cases of acute diarrhoea during infancy, bacterial infections are also well recognized as causes of them. 256 patients with diarrhoea due to a bacterial pathogen were studied to determine the importance of different epidemiologic factors. the study group comprised 151 boys (59%) and 105 girls (41%) aged between first month and 14 years. family history of acute diarrhoea was ... | 1991 | 2064148 |
| the role of the o antigen in adjuvant activity of lipopolysaccharide. | adjuvant activities of isogenic salmonella enterica, serovar typhimurium, o-6,7 and o-4,5,12 lipopolysaccharide (lps), lipid a and bordetella pertussis lps were compared by immunizing groups of mice subcutaneously with diphtheria and tetanus toxoid vaccine alone or mixed with one of the lps derivatives. five weeks later the mice were bled and the tetanus and diphtheria antibodies in the sera were measured. all the lps derivatives efficiently increased the antibody responses when compared to the ... | 1991 | 1778421 |
| salmonella enterica subspecies diarizonae bacteremia in an infant with enteritis--a case report. | the septicemia caused by the arizona group organism is rare and usually observed in adults with underlying diseases. in korea, salmonella infection is common, but a report of arizona infection is unknown. we isolated s. entercia subsp. diarizonae from blood of a 6-month-old infant. the serovar was determined as 28:z10:-, a rare one in america. the isolate was susceptible to ampicillin, chloramphenicol, cotrimoxazole and others. the patient rapidly recovered with ampicillin and gentamicin therapy ... | 1991 | 1781188 |
| positive regulator for the expression of mba protein of the virulence plasmid, pkdsc50, of salmonella choleraesuis. | a positive regulator was identified within a 2.3 kb fragment of the 6.4 kb mouse bacteremia region (mba region) of the virulence pkdsc50 plasmid of salmonella choleraesuis. sodium dodecyl sulphate polyacrylamide gel electrophoresis showed that escherichia coli k-12 carrying the recombinant plasmids of the 2.3 kb fragment produced mba1 protein with a molecular mass of 32 kda. the recombinant plasmids carrying a 4.1 kb fragment, the other part of 6.4 kb region, produced mba2 (32 kda), mba3 (70 kda ... | 1991 | 1795622 |
| relevance of inoculation route to virulence of three salmonella spp. strains in mice. | the virulence of three salmonella species strains was compared by the i.p. and i.v. routes in balb/c mice. salmonella choleraesuis, sl2824 (serogroup c1, o-6,7), was more virulent by the i.v. than i.p. route. a strain of s. typhimurium, sl1260 (serogroup b; o-1,4,12) was more virulent i.p. than i.v. while another strain, sl3201 (o-4,5,12) was equally virulent i.p. or i.v. the ld50 of each strain by either route correlated with the number of bacteria in the liver and spleen on day one after inocu ... | 1991 | 1795625 |
| salmonella infection of the biliary and intestinal tract of wild opossums. | bacteriologic cultures were taken from the mesenteric lymph nodes, biliary tract, blood, liver, spleen and pancreas of opossums (didelphis virginiana) obtained directly from the wild for use as research animals. the overall incidence of salmonellosis outside the intestinal tract was 61% among 18 opossums. salmonella was recovered from the gallbladder of six (33%) animals, indicating chronic biliary tract infection. among these six animals, translocation of salmonella to regional lymph nodes was ... | 1991 | 1849588 |
| [infection of a neoplastic ovarian cyst caused by salmonella enterica subspecies enterica, serotype enteritidis]. | 1991 | 1863613 | |
| [serotypes of salmonella enterica in sewage effluent from zaragoza. comparison with clinical isolates. 1982-1989]. | between 1982 and 1989, 560 salmonella enterica strains belonging to 63 serovars, were isolated from zaragoza urban sewage. during the same period of time there were 45 different serovars isolated from faeces of patients from a hospital (in the same city). only a reduced number of serovars comprised the majority of the human and environmental isolates. an approximately rectilinear relationship is shown between the isolated strains number and the number of different serovars, in non human salmonel ... | 1991 | 1867775 |
| characterization of a salmonella choleraesuis mutant that cannot multiply within epithelial cells. | a mutant of salmonella choleraesuis was identified that could invade (enter) and penetrate through polarized monolayers of caco-2 and mdck epithelial cells at normal levels but was defective for intracellular multiplication within these cells. it was also able to survive inside cultured j774 macrophage cells. these bacteria remained inside membrane-bound vacuoles, which coalesced at later times in the perinuclear region of the epithelial cell. this mutant exhibited slightly slower growth rates i ... | 1991 | 1913359 |
| seasonal variation in etiology of travelers' diarrhea. finnish-moroccan study group. | the etiology of travelers' diarrhea was studied in 579 adult finnish tourists participating in two packaged tours to morocco in the winter (n = 233) and fall (n = 346) of 1989. a research team accompanied the travelers, and a laboratory for enteric pathogens was established in agadir. at least one pathogen was found in 62% of the 60 diarrhea cases in winter and in 58% of the 111 diarrhea cases in fall. multiple pathogens were found less often in winter (8%) than in fall (21%, p less than .05). c ... | 1992 | 1730905 |
| separation of two lipopolysaccharide populations with different contents of o-antigen factor 122 in salmonella enterica serovar typhimurium. | lipopolysaccharide (lps) extraction from smooth-type salmonella enterica sv. typhimurium was carried out with the modified phenol/chloroform/petroleum ether method (volume ratio 5:5:8). in this procedure, lps was precipitated from 90% phenol sequentially with water and acetone to yield lps-h2o (minute amounts) and lps-ac (major amounts), respectively. chemical analyses of the lps fractions revealed that in the o antigen of lps-h2o position c4 of the d-galactose was extensively glucosylated, corr ... | 1992 | 1279361 |
| monoclonal antibodies against salmonella porins: generation and characterization. | monoclonal antibodies (mabs) were generated against porins, one of the major outer membrane proteins of salmonella typhi. six clones, designated mp1, mp2, mp3 (igg2ak), mpn4, mpn6 (igg1k) and mpn5 (igg2bk) were characterized by enzyme immunoassay (elisa) for their reactivity to porins from s. typhi, salmonella paratyphi a, s. paratyphi b, s. paratyphi c, salmonella choleraesuis, salmonella enteritidis, salmonella krefeld, salmonella panama, salmonella typhimurium, escherichia coli b, shigella fl ... | 1992 | 1280248 |
| pneumonia associated with salmonella choleraesuis infection in swine: 99 cases (1987-1990). | salmonella choleraesuis was isolated in pure or mixed bacterial cultures from 153 swine necropsied between jan 1, 1987 and dec 31, 1990. pneumonia was seen in 99 of 109 swine from which this bacterium was isolated in the absence of other pathogenic bacteria. pneumonia was seen more frequently than hepatitis, splenomegaly, or colitis. pleuropneumonia that was grossly indistinguishable from the pleuropneumonia associated with actinobacillus pleuropneumoniae was seen in 29 of 99 swine from which s ... | 1992 | 1289347 |
| [evaluation of household chemical disinfectants for vibrio cholerae el tor (non toxigenic strain)]. | the methodology of microbiological evaluation of disinfectants in permanently being questioned because the laboratorial protocols do not correspond to the real conditions under which these products are used. in 1985 the use-dilution method of aoac was adopted in brazil for microbiological qualification of chemical disinfectants for commercial purposes. domestic disinfectants are tested in this way against salmonella choleraesuis and staphylococcus aureus atcc strains, was chosen for this evaluat ... | 1992 | 1342521 |
| molecular analysis of a salmonella enterica group e1 rfb gene cluster: o antigen and the genetic basis of the major polymorphism. | salmonella enterica is highly polymorphic for the o antigen, a surface polysaccharide that is subject to intense selection by the host immune system. this polymorphism is used for serotyping salmonella isolates. the genes encoding o antigen biosynthesis are located in the rfb gene cluster. we report here the cloning and sequence of the 19-kb rfb region from strain m32 (serovar anatum, group e1) and compare it with that of strain lt2 (serovar typhimurium, group b). genes for biosynthetic pathways ... | 1992 | 1372579 |
| cloning and structure of group c1 o antigen (rfb gene cluster) from salmonella enterica serovar montevideo. | the salmonella enterica group c1 o antigen structure has a man-man-man-man-glcnac backbone with a glucose branch, which differs from the s. enterica group b o antigen structure which has a man-rha-gal backbone with abequose as side-chain. we have cloned the group c1 rfb (o antigen) gene cluster from serovar montevideo strain m40, using a low-copy-number cosmid vector. the restriction map of the group c1 (m40) rfb gene cluster was compared with that of group b strain lt2 by southern hybridization ... | 1992 | 1373435 |
| molecular analysis of the rfb gene cluster of salmonella serovar muenchen (strain m67): the genetic basis of the polymorphism between groups c2 and b. | the rfb (o antigen) gene cluster of group c2 salmonella differs from that of group b in a central region of 12.4 kb: we report the sequencing of this region of strain m67 (group c2) and a subsequent comparison with the central region of strain lt2 (group b). we find a block of seven open reading frames unique to group c2 which encode the o antigen polymerase (rfc) and the transferases responsible for assembly of the group c2 o antigen. the remaining rfb genes are common to strains m67 and lt2, b ... | 1992 | 1379320 |
| bacterial motility is a colonization factor in experimental urinary tract infection. | in an experimental urinary tract infection of the mouse, colonization of the urinary bladder by isogenic strains of salmonella enterica serovar typhimurium was found to depend on the motility of the bacteria. strains were obtained by genetic recombination between a highly motile o-6,7 and a poorly motile o-4,5,12 strain. the o antigen did not interfere with the colonization, whereas motility did; flagellated and motile o-6,7 and o-4,5,12 bacteria colonized the bladder equally well. | 1992 | 1379992 |
| the role of o-antigen polysaccharide in the activation of neutrophils by lipopolysaccharides of salmonella species. | activation of neutrophils by lipid a, o-antigen polysaccharides (ps) and smooth lipopolysaccharides (lps) isolated from salmonella choleraesuis (o-6,7) and salmonella typhimurium (o-4,5,12) was investigated. the methods used were assays for lysozyme release and for nitroblue tetrazolium (nbt) reduction which measures the level of oxidative metabolism of neutrophils. lps from both species stimulated neutrophils to the same extent in the presence of autologous plasma. in the absence of plasma only ... | 1992 | 1382904 |
| sequence and structural analysis of the rfb (o antigen) gene cluster from a group c1 salmonella enterica strain. | the rfb (o antigen) gene cluster of a group c1 salmonella enterica strain was sequenced; it comprised seven open reading frames which precisely replaced the 16 open reading frames of a group b strain. two genes of the mannose biosynthetic pathway were present: rfbk (phosphomannomutase) had a g+c content of 0.61 and had only 40% identity to rfbk of group b but was very similar to cpsg of the capsular polysaccharide pathway with 96% identity, whereas rfbm [guanosine diphosphomannose (gdp-man) pyro ... | 1992 | 1383393 |
| induction of gamma/delta t cells in murine salmonellosis by an avirulent but not by a virulent strain of salmonella choleraesuis. | to elucidate the relationship between the virulence of intracellular bacterium and its ability to induce gamma/delta t cells in the host during infection, we examined the differences in appearance of gamma/delta t cells in mice infected with salmonella choleraesuis virulent strain rf-1 carrying a virulence plasmid of 50 kb, and with avirulent strain 31n-1 cured of the 50-kb plasmid. the number of gamma/delta t cells in the peritoneal cavity was increased to a significant level on day 3 after an ... | 1992 | 1386870 |
| [focal infections caused by non-typhi salmonella: a review of our case series and comparison with other series]. | nine cases of local infection due to non typhi salmonella enterica, some of them of unusual localization, in 8 patients (mean age 64.9 +/- 12.4 years) attended in zamora's virgen de la concha hospital over a period of five years, are described. focal salmonellosis represented 1.5% of non-typhi salmonellosis cases in that period (9 out of 606 detected cases). 6 of the 8 patients (75%) showed a predisposing disease. in two patients the previous existence of gastroenteritis due to salmonella was as ... | 1992 | 1386936 |
| [molecular epidemiology of salmonella enterica poisoning: correlation of the serotype and protein profile and plasmid dna analysis]. | food-borne diseases nosocomial outbreaks due to salmonella enterica strains have a high incidence nowadays. we describe here an outbreak that occurred in july 1990 in the malaga university hospital, that only involves one shift of health care workers. salmonella enterica was detected in stool samples from hcw and a first analysis revealed the presence of two different antibiotic susceptibility patterns (resistotypes) among isolated strains. two months after the outbreak started, the cnvis (majad ... | 1992 | 1391014 |
| characterization and protective properties of attenuated mutants of salmonella choleraesuis. | we have constructed crp::tn10 and cya::tn10 salmonella choleraesuis mutants and their fusaric acid-resistant derivatives with deletions (delta) of the tn10 and adjacent dna sequences and found them to be avirulent and able to induce protection against a wild-type challenge in 8-week-old balb/c mice. mice survived infection with the crp and cya mutants at doses of more than 7 x 10(3) times the oral (p.o.) 50% lethal dose (ld50) and more than 8 x 10(2) times the intraperitoneal ld50 of the wild-ty ... | 1992 | 1398999 |
| salmonella choleraesuis subsp. indica serovar bornheim causing urinary tract infection. | an unusual salmonella species, s. choleraesuis subsp. indica serovar bornheim, was isolated from the urine of a patient with aplastic anemia, diabetes mellitus, and a healed urethral injury. an immune response to this isolate was demonstrated by whole-bacterial-cell agglutination. | 1992 | 1401026 |
| isolation and characterization of the aada aminoglycoside-resistance gene from salmonella choleraesuis. | the streptomycin- and spectinomycin-resistance gene of salmonella choleraesuis was cloned and its nucleotide sequence determined. the gene is 789 bases long, encoding a protein of a predicted size of 29,353 da. the gene product inactivated streptomycin and spectinomycin by an adenylation modification. it is homologous (c. 40% total identity) to streptomycin adenylyltransferase, a 3'(9)-o-nucleotidyltransferase (aad(3')(9)), which is encoded by the aada gene in escherichia coli, agrobacterium tum ... | 1992 | 1406282 |
| invasion of enterocytes in cultured porcine small intestinal mucosal explants by salmonella choleraesuis. | porcine small intestinal explants maintained in vitro were inoculated with salmonella choleraesuis to study the characteristics of its invasion of enterocytes. the explants were fixed at selected intervals for up to 12 hours after inoculation and examined by conventional light microscopy, immunoperoxidase staining, and transmission electron microscopy. although there was diffuse loss of villous enterocytes during the first hour of incubation, the villi were reepithelialized by the end of 2 hours ... | 1992 | 1416346 |
| the role of 36 megadalton plasmid of salmonella enteritidis for the pathogenesis in mice. | the pathogenesis associated with the 36 megadalton (md) plasmid of salmonella choleraesuis subsp. choleraesuis serovar enteritidis (s. enteritidis) was assessed by using the plasmid-containing strain al1190, plasmid-cured strain al1192, and plasmid-reintroduced strain al1193. after oral inoculation of strain al1190 or al1192 to c57bl/6 mice, mesenteric lymph nodes, spleens, and livers were examined for the numbers of viable bacteria and for the histopathological changes. the numbers of bacteria ... | 1992 | 1420563 |
| monoclonal antibodies that detect live salmonellae. | nine immunoglobulin g and nine immunoglobulin m murine monoclonal antibody-producing hybridomas reactive with live salmonella bacteria were obtained from several fusions of immune spleen cells and sp2/0 myeloma cells. the antibodies were selected by the magnetic immunoluminescence assay. the monoclonal antibodies were reactive with serogroups a, b, c1, c2, d, e, and k and salmonella choleraesuis subsp. diarizonae. each monoclonal antibody proved to be reactive with a distinct serotype. clinical ... | 1992 | 1476430 |
| effect of 50 kilobase-plasmid, pkdsc50, of salmonella choleraesuis rf-1 strain on pig septicemia. | salmonella choleraesuis strains with and without 50-kilobase plasmid (pkdsc50) were intravenously inoculated into yorkshire pigs. by the inoculation of 7.2 x 10(5) - 3.5 x 10(7) cells, rf-1 strain with pkdsc50, but not 31n-1 strain without the plasmid, caused a septicemia. the inoculation of 8.7 x 10(9) rf-1 cells killed pigs at 2-4 day postinfection with severe hemorrhage on the whole body. pigs with a similar number of 31n-1 cells (8.3 x 10(9) cells), showed milder hemorrhage, and they died at ... | 1992 | 1477166 |
| molecular typing of salmonella enterica subsp. enterica serovar wien by rrna gene restriction patterns. | analysis of digested dna from 40 salmonella enterica subsp. enterica serovar wien isolates revealed five different rrna gene restriction patterns for hindiii (h1 to h5) and five for psti (p1 to p5). the isolates had been selected on the basis of the year of isolation, the geographic origin and the antibiotic resistance pattern and were distinguished as follows: a) 13 pre-epidemic isolates from different french towns in 1958-69 and from senegal in 1968; b) 7 epidemic isolates from algiers in 1969 ... | 1992 | 1488554 |
| [increase in the incidence of the typhimurium serotype of salmonella enterica (i) versus the classic enteritidis serotype]. | 1992 | 1489802 | |
| [detection of enterotoxin production in bovine salmonella isolates]. | 54 salmonella-strains (7 serovars) of bovine origin isolated from faecal samples, rectal swabs as well as from organs of emergency-slaughtered or dead animals were tested for enterotoxin production (heat-labile and heat-stable) in rabbit-ileal-loop-assay (rilt), skin-permeability-factor-test (hpt), cho- and y1-cell-culture-assay and in baby-mouse-test (bmt). the cell-free supernatants (cfs) were used in the tests. the y1-cells did not respond to the salmonella toxins. while the rilt was suitable ... | 1992 | 1497581 |
| phosphatidylserine found in intestinal mucus serves as a sole source of carbon and nitrogen for salmonellae and escherichia coli. | salmonella choleraesuis (a pig pathogen), salmonella typhimurium (a virulent strain in mice), and three strains of escherichia coli (including a human enterohemorrhagic strain, a human urinary tract isolate, and a human fecal isolate) grew as well in vitro utilizing the lipids derived from mouse cecal mucus as the sole source of carbon and nitrogen as they did in mouse crude cecal mucus. further analysis of the total lipid extracts of mucus dialysates showed that the acidic lipid fraction suppor ... | 1992 | 1500206 |
| characterization of a salmonella choleraesuis isolate after repeated neutrophil exposure. | salmonella choleraesuis strain 38 (glycerol-positive fermentation) was repeatedly exposed to porcine neutrophils in an attempt to mimic in vivo conditions of the host immune system. after phagocytosis, viable intracellular s choleraesuis were isolated and the process was repeated at least 5 times. a fifth-passage strain-38 neutrophil-adapted clone, 38pmna-5x, was isolated, and was compared with the parent wild-type strain 38 for changes. strain 38pmna-5x had increased resistance to killing by hy ... | 1992 | 1510308 |
| in vivo isolation of salmonella choleraesuis from porcine neutrophils. | seventy-five pigs from 4 facilities were examined for salmonella choleraesuis by use of bacteriologic culture of feces, blood, wbc (buffy coat), mononuclear leukocytes, and neutrophils. the organism was isolated from 0 of 75 fecal samples, compared with isolation from 39 of 75 purified neutrophil preparations. of the pigs that did not have salmonella isolated from feces or blood, but had s choleraesuis isolated from neutrophils, 6 were further examined. these pigs from 2 groups again had culture ... | 1992 | 1510309 |
| plasmid profile analysis, phage typing, and antibiotic sensitivity of salmonella dublin from clinical isolates in the united states. | one hundred clinical isolates of salmonella choleraesuis subsp. choleraesuis serovar dublin (salmonella dublin) were examined for phage sensitivity, antibiotic resistance patterns, and plasmid content. computer analysis of the lysis patterns observed by using 27 typing phages divided the s. dublin isolates into 26 groups. one lytic pattern (designated pattern 16) contained 52% of the isolates examined whereas 16 isolates had unique patterns, and nine patterns had fewer than ten members. although ... | 1992 | 1514237 |
| salmonella enterica: infection, cross infection and persistence within the environment of a broiler parent stock unit in denmark. | a total of three hundred and ninety nine isolates of salmonella enterica were recovered from 3106 cloacal swab cultures, chicken carcasses and environmental samples taken from a broiler breeding farm over six successive generations. s. enterica serovars berta (s. berta), 4,12:b:-, taksony, binza and typhimurium were identified. one hundred and seventy one strains of s. berta were analysed by plasmid profiling to determine the epidemiological relationships between isolates from each generation an ... | 1992 | 1520963 |
| non-tropical pyomyositis caused by salmonella enterica enterica in an immunocompetent patient. | 1992 | 1526245 | |
| enzymatic synthesis and isolation of thymidine diphosphate-6-deoxy-d-xylo-4-hexulose and thymidine diphosphate-l-rhamnose. production using cloned gene products and separation by hplc. | a two-step enzymatic synthesis of dtdp-l-rhamnose is developed using enzymes from sonicated extracts of cultures of escherichia coli k12 strains harboring plasmids containing different parts of the rfb gene cluster of salmonella enterica lt2. the intermediate dtdp-6-deoxy-d-xylo-4-hexulose was isolated after a 1-h reaction, using only dtdp-d-glucose and dtdp-d-glucose 4,6-dehydratase, followed by protein precipitation and desalting by gel chromatography (yield 89%). in a two-step reaction using ... | 1992 | 1541269 |
| anti-ifn-gamma-treated mice--a model for testing safety of live salmonella vaccines. | salmonella enterica serovars typhimurium and enteritidis are facultative intracellular pathogens which cause in mice a disease similar to human typhoid fever caused by serovar typhi. an essential phase in the infection process is bacterial replication inside cells of the liver and spleen; the rate of replication is restricted by interferon-gamma (ifn-gamma) produced early in the infection. in the present study the effect of ifn-gamma was neutralized in vivo with monoclonal antibody and the fate ... | 1992 | 1561831 |