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the ventral metacarpo- and metatarso-phalangeal sesamoid bones: comparative anatomy and evolutionary aspects.a study of comparative anatomy was made to determine the distribution of metapodo-phalangeal sesamoid bones among the different mammalian species for a better understanding of the variations and origins of these bones in man, and that in the context of renewed interest in non-metrical characteristics of the post-cranial skeleton in physical anthropology. the material used included 457 non-primate mammalian skeletons divided into 18 orders, 63 families, and 161 genera, 211 non-human primate skele ...19883224803
[behavior of bronchial reactivity in 50 children before and after exclusion of food allergens].in this study the author has purposed to demonstrated that the food allergens increase the bronchoreactivity. this studied group was formed by 50 boys with chronic rhinitis and/or nasal perennial and/or laryngeal and bronchial obstruction, unresponsive to aeroallergens but responsive to food allergens determined with fbst test. this test is performed by making the patient go on an anallergic diet (formed by tapioca's flour, rabbit, olive oil, beets, lettuce, pear, sugar, salt for seven days). th ...19883244541
the oral allergy syndrome.we have studied 262 patients suffering from hay fever and oral allergy syndrome after fruit and vegetable ingestion. the history and the results of rast and skin test for pollen showed a contemporary presence of systemic and local symptoms in most of the patients; a close connection between age of onset of hay fever and oral allergy syndrome; a frequent association between allergy to some pollens and some vegetables, such as between apple, carrot, pear, cherry with birch pollen and tomato, melon ...19883264668
types of neurons in nucleus olivaris inferior of the european bison.the studies were carried out on the medullae oblongatae of four european bisons. preparations made by means of the golgi technique, as well as preparations stained by the klüver-barrera methods, were used. two types of neurons were distinguished in nucleus olivaris inferior of the european bison. type i (about 90% of neurons) are multipolar cells whose perikaryons measure from 25 to 40 microns. the multipolar cells generate 5-6 thick dendrites which next give off a number of branches. the dendri ...19882458411
occurrence of lipofuscin pigment granules and "dense microspheres" in the spinal cord of young cats treated with beta,beta'-iminodipropionitrile (idpn).spinal cords of cats treated with the neurotoxic compound beta,beta'-iminodipropionitrile (idpn) were observed to contain rounded homogeneous bodies, 1-12 microns in diameter, termed "dense microspheres" (dms). these bodies, absent in control animals, were consistently found only in the ventral horns. no relationship with blood vessels was evident. when stained with pas and a modified von kossa's silver nitrate technique, dms remained negative, showing only very slight metachromasia in some tolu ...19882980224
chronic disease in goats orally infected with two isolates of the caprine arthritis-encephalitis lentivirus.newborn saanen goats were orally infected with two distinct biologically cloned isolates of the caprine arthritis-encephalitis lentivirus (caev), designated caev(63) (crawford tb, adams ds, cheevers wp, cork lc: science 207:997, 1980) and caev (co) (cork lc, hadlow wj, crawford tb, gorham jr, piper rc: j infect dis 129:134, 1974; narayan o, clements je, strandberg jd, cork lc, griffin de: j gen virol 50:69, 1980). all infected goats seroconverted for antibodies to caev, and there were no serolog ...19882835548
ultrastructural biology of leishmania (viannia) panamensis (=leishmania braziliensis panamensis) in lutzomyia gomezi (diptera: psychodidae): a natural host-parasite association.the development of leishmania (viannia) panamensis in a natural sand fly host, lutzomyia gomezi, was studied by light and transmission electron microscopy. new aspects of peripylarian parasite behavior and morphology in the sand fly gut, early bloodmeal stages, and ultrastructural development in the anterior gut were documented. eight distinct morphological forms were observed in the life cycle of the parasite within the insect. in the bloodmeal, amastigotes (1) transformed into stumpy promastig ...19892916730
distribution of somatostatin and glucagon immunoreactive cells in the gastric mucosa of some cartilaginous fishes.the comparative distribution of somatostatin- and glucagon-like-containing cells in the histomorphologically different gastric mucosae of the cartilaginous fishes heptranchias perlo, raja asterias, scyliorhinus canicula, squatina aculeata, and torpedo marmorata was immunocytochemically studied to demonstrate a possible interrelationship between these endocrine cells and/or other endocrine or nonendocrine cells. in the gastric mucosa, these open-type glucagon and somatostatin immunoreactive cells ...19892570006
a case of calcitonin-producing parathyroid adenoma with primary hyperparathyroidism.a 57-year-old female was admitted to our hospital with general lassitude, loss of appetite, nausea, upper abdominal pain, thirst, polydipsia and polyuria. on admission, she had an asymmetrical pear-shaped tumor in the right supraclavicular region and severe hypercalcemia. plasma c-pth was elevated to 22.72ng/ml. plasma calcitonin was also elevated to 336 pg/ml. she died of respiratory and cardiac failure of two weeks after admission without any positive response to the treatment, including hemod ...19892585894
organization of ribosomal rna genes in alternaria alternata japanese pear pathotype, a host-selective ak-toxin-producing fungus.dna encoding ribosomal rna (rrna) of alternaria alternata japanese pear pathotype has been cloned in lambda replacement vector, lambda fix. restriction endonuclease mapping and southern hybridization with the 18s and 28s rrnas of saccharomyces cerevisiae revealed the a. alternata rdna to be tandemly repeating 8.15-kilobase pair unit. the restriction fragments of the unit were then subcloned in the plasmid vector bluescribe m13- and partially sequenced. the determined sequences were compared with ...19892697467
monospermy and polyspermy after partial zona dissection of reinseminated human oocytes.partial zona dissection (pzd), a zona drilling method that uses mechanical force to open the zona pellucida while the oocyte is shrunken in a sucrose solution, was applied to 121 unfertilized 1-day-old mature human oocytes prior to reinsemination. the 115 surviving oocytes were divided into three groups in which the duration between sucrose addition and reinsemination was varied: i) less than 20 minutes, ii) 21 to 45 minutes, and iii) longer than 45 minutes. there was a trend toward a reduced fe ...19892777173
ultrastructural development of leishmania chagasi in its vector, lutzomyia longipalpis (diptera: psychodidae).the development of leishmania chagasi, etiologic agent of american visceral leishmaniasis, was studied by light and electron microscopy in the gut of the sand fly, lutzomyia longipalpis, a natural vector. new aspects of suprapylarian leishmania behavior were elucidated. in the sand fly midgut, amastigotes transformed into promastigotes (division promastigote i) during a first division sequence within the bloodmeal. secondary division of these promastigotes resulted in a second form (division pro ...19892802019
shape changes in caprine erythrocytes exposed to chlorpromazine and lysolecithin.stomatocytic and echinocytic transformations of caprine erythrocytes were studied in vitro using chlorpromazine as a stomatocyyic agent and lysolecithin as an echinocytic agent. morphologic changes in erythrocytes generally varied with the cell shape and the concentration of the substances used. discoytic, triangular, and pear-shaped red cells common to normal goats, exhibited classical stomatocytic and echinocytic changes with formation of spherostomatocytes, sphero-echinocytes, and spherocytes ...198915156515
inferior vena caval occlusion: characteristic radiographic changes on excretory urography and barium enema examination.acute occlusion of the inferior vena cava produces characteristic radiographic findings on intravenous urogram and barium enema examination. the urinary bladder is compressed anteriorly and superiorly giving an inverted pear-shaped appearance, and the distal ureters are medially displaced. barium enema examinations demonstrate narrowing and elevation of the rectosigmoid region along with an increase in the presacral space. four cases are described to illustrate the usefulness of these findings i ...1989401585
somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (pyrus communis var. pyraster l.) and colt cherry (prunus avium x pseudocerasus).mesophyll protoplasts of wild pear (pyrus communis var. pyraster l., pomoideae) were chemically fused with cell suspension protoplasts of cherry rootstock colt (prunus avium x pseudocerasus, prunoideae), following an electroporation treatment of the separate parental protoplast systems. fusion-treated protoplasts were cultured, on modified k8p medium, where it had been previously established that neither parental protoplasts were capable of division. somatic hybrid calli were recovered and, foll ...198924227027
adventitious shoot regeneration from leaf tissue of three pear (pyrus sp.) cultivars in vitro.to develop an adventitious regeneration system for pear cultivars, several experiments were conducted with 2 cultivars of pyrus communis l. ('seckel' and 'louise bonne') and one cultivar of p. bretschneideri rehd. ('crystal pear'). half-leaves, taken from shoots proliferating on lepoivre medium, were plated in petri-dishes on medium supplemented with various combinations of cytokinins and auxins. cultures of the above cultivars had been established from mature trees. among the growth regulators ...198924240463
[successful treatment of a cheilothorax in lymphangiomatosis of the ribs (gorham-stout syndrome)].in 1955 gorham and stout described the syndrome of lymphangiomatosis of the bone resulting in widespread monofocal osteolysis in the skeletal system. the results of therapy are difficult to evaluate as the disease may come to a sudden stand-still. if angiomatosis arises near the thorax, pleural effusion occurs due to simultaneous involution of the thoracic duct. this complication has a fatal outcome and effective treatment is required. a case is reported in a 61-year-old male in whom radiotherap ...19892740881
cell wall metabolism in ripening fruit: iv. characterization of the pectic polysaccharides solubilized during softening of ;bartlett' pear fruit.fractionation of pectic polysaccharides from the juice of ripening ;bartlett' pears (pyrus communis) gave two general types of polyuronides. the major type was a homogalacturonan (hga) whose molecular weight decreased upon ripening. the other type comprised heteropolymers composed of various amounts of arabinose, rhamnose, and galactose. treatment of the major arabinose-containing heteropolymeric fraction of high molecular weight (400,000) with a pear exo-polygalacturonase to degrade contaminati ...198916666715
open assessment of the safety and efficacy of thioridazine in the treatment of patients with borderline personality disorder.a 12-week open study was conducted to assess the possible efficacy and safety of low-dose thioridazine in 11 outpatients (8 women, 3 men) with borderline personality disorder (bpd) diagnosed according to dsm-iii-r (american psychiatric association 1987) and the diagnostic interview for borderline patients (dib; gunderson et al. 1981) criteria. mean thioridazine dose averaged 92 mg per day across the duration of this study. at endpoint, there was a significant reduction in brief psychiatric ratin ...19892631134
[pseudotumor of the shoulder after massive osteolysis (gorham-stout)]. 19892536489
the initiation and organization of microtubules in germinating pear (pyrus communis l.) pollen.to study microtubule organization in germinating pear (pyrus communis l., cv., bartlett) pollen, we removed the pollen wall by freeze-fracturing before treating the resultant pollen protoplasts by conventional immunofluorescence procedures. results reveal that axial bundles of microtubules are present in the generative cell of both inactivated and activated pollen grains. microtubules are not present in the vegetative cells of inactivated pollen, but they are present in the vegetative cells of a ...19902081552
[the psychopathologic picture of incipient schizophrenia in the male population at approximately 20 years of age].as the diagnosis of initial schizophrenic psychosis is sometimes difficult and the diagnosis of schizophrenia is based on psychopathological characteristics the author used the relative homogenity of their cases for comparison with various known concepts and scales in an attempt to contribute to the serious problem of assessment of this nosological unit. psychopathological descriptions of 45 cases of first hospitalizations on account of schizophrenia of men aged 18-24 years were investigated wit ...19902261655
[iconographic rubric. gorham disease]. 19902078112
musculoskeletal case of the day. gorham syndrome of the right clavicle and scapula. 19902110757
radiotherapy of morbus gorham-stout: the biological value of low irradiation dose.morbus gorham-stout is a very rare disease. a patient with this disease was treated at the university clinic of radiobiology in vienna. the clinical and radiological findings place special emphasis on the radiotherapeutic treatment, and the biological basis of the recommended dose is discussed.19902334833
massive pelvic osteolysis in the gorham-stout syndrome.massive osteolysis is a very rare tumour-like lesion usually affecting young adults. there are 132 reported cases which we have reviewed, including 31 single cases of pelvic involvement. the term "massive osteolysis" is based on the typical radiological findings, such as increasing translucency and loss of bone density. the diagnosis of the gorham-stout syndrome must be confirmed by the microscopic finding of intramedullary vascular structures. our patient was a 23 year old white caucasian, who ...19902076919
the effect of storage of canned juices on content of the metals fe, cu, zn, pb, sn, al, cd, sb and ni.the increase in concentration of iron, copper, zinc, lead, antimony, aluminium, cadmium, tin and nickel over a 2 year's time of juices of peach (prunus persica), pear (pyrus communis), apricot (prunus vulgaris) and apple (malus pumila) was determined. the results show a considerable increase in fe, cu, pb, zn and sn with time, while the change in al, cd, ni and sb is negligible.19902366854
three new species of eimeria (apicomplexa: eimeriidae) from apalone spinifera pallidus (testudines: trionychidae) in texas, with a redescription of e. amydae.three new species of eimeria are described from pallid spiny softshells, apalone spinifera pallidus, collected in north-central texas. oocysts of eimeria spinifera n. sp. were found in the feces of 3/9 (33%) turtles and are subspheroid, ellipsoid, or pear-shaped, 16.3 x 14.0 (14-19 x 12-18) microns, with a thin, single-layered wall; shape index 1.2 (1.1-1.3). a micropyle is absent, but an oocyst residuum is present; polar granule present in 16% of the oocysts. sporocysts are elongate-ovoid, 10.3 ...19902380856
tissue culture and top-fruit tree species.the commercial cultivation of rosaceous fruit trees (e.g., pear, apple, cherry, peach, plum) relies heavily upon the quality and performance of the rootstocks. this is even more the case now that self-rooted scions produce larger trees with a longer juvenile phase (1). it would, therefore, be of special interest for the fruit breeder to have general purpose rootstocks with a wide ecophysiological adaptation and high compatibility coupled with early cropping. in addition, many of the older and hi ...199021390607
evidence for quantitative responses during co-culture of pyrus communis protoplasts and erwinia amylovora.mesophyll protoplasts were isolated from axenic shoot cultures of pear cultivars, exhibiting different degrees of susceptibility to fire blight infection at the whole plant level and they were co-cultured with the wild-type strain cfbp 1430 of erwinia amylovora, and with an avirulent transposon mutant of the former (pmv 6046). results, as assessed in terms of the effects of bacteria on protoplast viability, the time to the onset of divisions, the percentage of the originally cultivated protoplas ...199024226825
[contractile function, extracardiac regulation and morphological changes in the hearts of rats in the dynamics of a long-term continuously acting stressor situation].24 hrs of the overpopulation stress effect induced a set of shifts typical for an acute stress syndrome in rats. within 5 days the organism mobilizes the cholinergic stress-limiting system functioning for 15 days, limiting and normalizing the contractile function (cf) of the heart, and eliminating the contracture damages of the myocardium on the 30th day. starting from the 30th day of the stress effect, adrenergic features of the extracardiac regulation (er) re pear, the cf parameters increase, ...19901966085
immunohistochemical localization of glutamine synthetase in mesencephalon and telencephalon of the lizard gallotia galloti during ontogeny.the immunohistochemical localization of glutamine synthetase, an astrocyte marker in mammals, was determined in the telencephalon and mesencephalon of the lizard gallotia galloti during development by using an antiserum raised against chicken brain glutamine synthetase. ependymal glial cells and their radial processes were glutamine synthetase immunoreactive, and they were present also in the adult. immunoreactivity was also detected in two populations of scattered cell bodies, each preferential ...19901970551
a novel endocrine cell type in the guinea-pig gastric mucosa: cellular source of pro-enkephalin-derived peptides. a histochemical, immunohistochemical, and electron microscopical characterization.a novel endocrine cell type has been identified in the guinea-pig gastric mucosa which preferentially occurs in the oxyntic area. cells of this type exhibit immunoreactivities for bovine adrenal medulla dodecapeptide (bam-12p) and in many cases for met-enkephalin and are thus presumed to contain a pro-enkephalin-like precursor protein. systematic immunohistochemical investigations show that these cells do not contain immunoreactivities for various enteric hormones, neuropeptides and biogenic ami ...19901972145
localization of neuropeptide-immunoreactive neurons in the human retina.light microscopic immunocytochemistry was utilized to localize populations of neurons in the human retina immunoreactive for the following neuroactive peptides: substance p (sp), vasoactive intestinal polypeptide (vip), somatostatin (som) and lant-6-(h-lys-asn-pro-tyr-ile-leu-oh), a hexapeptide which is identical to the c-terminal half of neurotensin except for the amino acid substitutions lys/arg and asn/arg. the majority of sp immunoreactive cells were amacrine cells whose pear-shaped or oval ...19901699634
experimental life history of spirometra erinacei.the complete life cycle of spirometra erinacei has been experimentally maintained in the laboratory. the cyclops were reared as the first intermediate host, and the tadpoles of rana nigromaculata as the second intermediate host. icr mice were used as another second host. the experimental definitive hosts were dogs and cats. maturation and hatching of the eggs took 8 to 14 days by incubation at 29 degrees c. the coracidium measured 43.8 x 36.9 microns. mesocyclops leuckarti and eucyclops serrulat ...19902095198
cloning of a large gene cluster involved in erwinia amylovora cfbp1430 virulence.phage mudiipr13 insertional mutagenesis of erwinia amylovora cfbp1430 allowed us to isolate 6900 independent cmr mutants. the frequencies of different auxotrophs in this population indicated that mudiipr13 had inserted randomly in e. amylovora. screening of 3500 cmr mutants on (i) apple calli and (ii) pear and apple seedlings led to the isolation of 19 non-pathogenic prototrophic single mutants, four of which expressed a lacz+ hybrid protein. expression of the fusion proteins was temperature sen ...19902117695
effect of the new spacer-device on the deposition of the inhaled metered dose aerosol.the effect of the new spacer-device on the in vitro and in vivo deposition of inhaled drug particles was studied. the in vitro deposition of beclomethasone dipropionate 250 micrograms/dose aerosol administered either through the conventional aerosol actuator with the short plastic mouthpiece or through the new pear-shaped spacer-device was evaluated with the modified cascade impactor method. for the in vivo study the disodium cromoglycate particles were labelled with a pure gamma-radiator 99mtc ...19902129249
the rcsa gene from erwinia amylovora: identification, nucleotide sequence, and regulation of exopolysaccharide biosynthesis.rcsa is a positive activator of extracellular polysaccharide synthesis in the enterobacteriaceae. a cosmid clone containing the rcsa gene from erwinia amylovora was identified by its ability to restore mucoidy to an e. stewartii rcsa mutant. the rcsa gene was subcloned on a 2.2-kilobase hindiii-psti fragment that hybridized with an e. stewartii rcsa probe and complemented e. stewartii and escherichia coli rcsa mutants. in addition, the cloned e. amylovora rcsa gene stimulated expression of cps:: ...19902131100
pectin isolated from prickly pear (opuntia sp.) modifies low density lipoprotein metabolism in cholesterol-fed guinea pigs.the effect of prickly pear soluble fiber on low density lipoprotein (ldl) metabolism was investigated by feeding male guinea pigs either a nonpurified diet containing 0.25% cholesterol (hc diet) or the hc diet + 1% prickly pear pectin (hc-p diet). plasma cholesterol levels were significantly decreased by the hc-p diet, with a 33% decrease in ldl levels (p less than 0.02) and an increase in ldl density. hepatic free and esterified cholesterol levels were reduced 40 and 85%, respectively (p less t ...19902231018
auditory epithelial migration on the human tympanic membrane: ii. the existence of two discrete migratory pathways and their embryologic correlates.the pathways of movement of dye-markings on the tympanic membranes of 30 ears of 15 volunteers were investigated by photography with a hopkin's rod. two discrete pathways were identified. movement on the pars tensa was centrifugal in all directions from the edge of the handle of malleus surface. this supported our previous suggestion that pars tensa epithelial migration is based on growth of the meatal plate. movement on the more central part of the handle surface itself, however, was always upw ...19902260527
structural and antigenic analysis of chlamydia pneumoniae.several isolates of chlamydia pneumoniae were compared with each other and to chlamydia trachomatis and chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. protein profiles of the c. pneumoniae isolates appeared to be the same and were distinct from the other chlamydia species. a 39.5-kilodalton (kda) protein, similar in molecular weight to the major outer membrane proteins (momp) of c. trachomatis and c. psittaci, was found in the sarkosyl-insoluble ...19902294060
the effect of whole ejaculate filtration on the morphology and the fertility of bovine semen.a sephadex g-15 filtration method was developed to remove abnormal and nonmotile bull sperm from an entire ejaculate. the efficiency of filtration was determined by adding freeze-killed sperm to the ejaculate or using ejaculates with elevated numbers of abnormal cells induced by scrotal insulation. a fertility trial, using split ejaculates, compared fertility of filtered and unfiltered semen. after filtration, samples with 0, 25, and 50% killed cells added contained 77 to 81% motile cells. addit ...19902312886
morphology of the human olfactory epithelium.the human olfactory epithelium has been previously studied with scanning electron microscopy; however, most studies have been limited to examining the epithelial surface. in an attempt to examine structures below the surface, we scanned epithelial fractures that occurred during tissue preparation. this made it possible to obtain unique three-dimensional images of cell profiles from the mucosal surface through the full depth of the epithelium. we examined supporting cells, olfactory neurons, basa ...19902376627
suspected avocado (persea americana) poisoning in goats.a herd of 15 cameroon goats was suspected of having been poisoned by eating leaves of the fuerte variety of avocado pear (persea americana). two of the affected goats were examined clinically, while necropsies were carried out on 3 of the 4 that had died. the most significant clinical findings were tachycardia, hyperpnoea and evidence of lung oedema. at necropsy severe lung oedema, hydrothorax and hydropericardium were present. severe myocardial degeneration, necrosis and fibrosis were the major ...19911770497
ultrastructure of chlamydia pneumoniae in cell culture.the electron microscopic appearance of chlamydia pneumoniae elementary bodies with pear-shaped, loose outer membrane has been suggested as one criterion of its classification as a new chlamydial species. the study of the original strain tw 183 in lcl 929 and hl cells and a low-passage isolate of kajaani-6 isolate in hl cells revealed spherical compact elementary bodies common to other chlamydia.19911778435
three-dimensional structure of myosin subfragment-1 from electron microscopy of sectioned crystals.image analysis of electron micrographs of thin-sectioned myosin subfragment-1 (s1) crystals has been used to determine the structure of the myosin head at approximately 25-a resolution. previous work established that the unit cell of type i crystals of myosin s1 contains eight molecules arranged with orthorhombic space group symmetry p212121 and provided preliminary information on the size and shape of the myosin head (winkelmann, d. a., h. mekeel, and i. rayment. 1985. j. mol. biol. 181:487-501 ...19911869586
scanning electron microscopic study of odontoblasts and circumpulpal dentin in a human tooth.two combined scanning electron microscopic methods including modified fixative procedures were used for studying the morphologic aspects of the odontoblasts and the corresponding dentinal wall at different endodontic levels. the odontoblasts were tightly packed in the pulp horn, where they assumed pear-shaped profiles and, from crown to apex, successively looked spindle shaped, club shaped, and globular. their number decreased in the pulp radicular portion, where the interglobular spaces were en ...19911923448
first heterotransplantation of a human carcinoid tumor into nude mice.the first successful heterotransplantation of a human carcinoid tumor into nude mice is reported. csh, a voluminous hepatic metastasis of a primary bronchial carcinoid tumor (csb) was resected and transplanted into three irradiated nude (swiss-nu/nu) mice both by subcutaneous (sc) and intramuscular (im) routes; the success rate was five of six. heterotransplanted tumors took 4 to 5 months to appear in the mice and 1 month to attain a width of 0.5 cm. both human and mouse tumors (named csh-sc and ...19911649689
protein phosphatase 2a in saccharomyces cerevisiae: effects on cell growth and bud morphogenesis.we have cloned three genes for protein phosphatases in the yeast saccharomyces cerevisiae. two of the genes, pph21 and pph22, encode highly similar proteins that are homologs of the mammalian protein phosphatase 2a (pp2a), while the third gene, pph3, encodes a new pp2a-related protein. disruptions of either pph21 or pph22 had no effects, but spores disrupted for both genes produced very small colonies with few surviving cells. we conclude that pp2a performs an important function in yeast cells. ...19911656215
nucleotide sequence and taxonomic value of the major outer membrane protein gene of chlamydia pneumoniae iol-207.chlamydia pneumoniae iol-207 genomic dna was hybridized with a 1.5 kb labelled dna probe containing the 3' region of the coding sequence for the major outer membrane protein (momp) of c. trachomatis serovar l1. an 8.5 kb bg/ii fragment containing the complete momp gene was cloned into lambda embl3. two hybridizing ecori fragments were sub-cloned into the lambda zap ii cloning vector and the resulting plasmids were used as templates for sequencing both strands of the c. pneumoniae momp gene. comp ...19912033374
glutamic acid decarboxylase (gad)-immunoreactive structures in the adult human lateral geniculate nucleus.the distribution of glutamic acid decarboxylase (gad)-like immunoreactivity in the lateral geniculate nucleus (lgn) of the human adult was studied in vibratome sections (50-60 microns thick) using the avidinbiotin-peroxidase method. the tissue was obtained at autopsy from five individuals without any known neurological disorders. only few gad-immunoreactive neurons were present in the layers of the lgn, even less in the interlaminar zones. the numerical density of gad-immunoreactive neurons and ...19912035846
identification of a new viroid as the putative causal agent of pear blister canker disease.a small rna species with the structural and functional properties characteristic of viroids has been isolated from three different pear sources each of which induced symptoms of the pear blister canker (pbc) disease when indexed in the pear indicator a 20. a close association between this rna and pbc disease was established, since two of the three studied sources were known to be affected by this malady only, and the viroid was not detected in healthy pear tissue. moreover, the pbc-associated vi ...19912045786
stereographic and stereometric study of sebaceous gland hyperplasia in rabbit pinnas induced by topically applied substances.tetradecane (td), testosterone (ts), and dimethyl sulfoxide (dmso) were separately inuncted on rabbit pinnas once a day; the pinnas were biopsied on days 1, 3, 7, and 28. untreated pinnas and squalane-treated pinnas were used as controls. three-dimensional images of sebaceous glands were reconstructed from their serial histologic sections using a computer-image analysis system. by stereometry, the sebaceous gland volumes gradually increased during the course of treatments with ts and dmso, where ...19912056196
[demonstration of sensor nervous endings in the rat thyroid gland].the nerve sensitive endings or receptors of the thyroid gland have been studied in twenty adult male rats. their morphology, their acetyl-cholinesterase specific activity, their mono-amino-oxidase activity and vip were investigated. few but constant nerve sensitive endings were observed in the thyroid gland. these receptors were subdivided by their morphology in the next groups: pear-shaped receptors with capsule; capsuled spherical receptors located near vascular walls; ovoidal receptors with c ...19911366352
the effect of different atmospheric ozone partial pressures on photosynthesis and growth of nine fruit and nut tree species.nursery stock of peach (prunus persica l. batsch, cv. o'henry), nectarine (p. persica l. batsch, cv. fantasia), plum (p. salicina lindel., cv. casselman), apricot (p. armeniaca l., cv. tilton), almond (p. dulcis mill., cv. nonpareil), prune (p. domestica l., cv. improved french), cherry (p. avium l., cv. bing), oriental pear (pyrus pyrifolia rehd., cv. 20th century), and apple (malus pumula mill., cv. granny smith) were planted in open-top chambers on april 1, 1988 at the university of californi ...199114972900
host suitability of eight prunus spp. and one pyrus communis rootstocks to pratylenchus vulnus, p. neglectus, and p. thornei.the effects of pratylenchus vulnus on rootstocks of eight commonly used prunus spp. and one pyrus communis were evaluated under greenhouse conditions during a 15-month period. in a first experiment, two almonds (moncayo and garrigues), one peach (gf-305), and two peach-almond hybrids (gf-677 and adafuel) inoculated with 2,000 nematodes per plant proved to be good hosts of p. vulnus. highest (p < 0.05) numbers of nematodes per gram of fresh root weight were recovered from adafuel and gf-677. root ...199119283165
meloidogyne lusitanica n. sp. (nematoda: meloidogynidae), a root-knot nematode parasitizing olive tree (olea europaea l.).a root-knot nematode from portugal, meloidogyne lusitanica n. sp., is described and illustrated from specimens obtained from olive trees (olea europaea l.). females of the new species have a characteristic perineal pattern with medium to high trapezoidal dorsal arch with distinct punctuations in the tail terminus area. the excretory pore is located posterior to the stylet, about 1.5-2.5 stylet lengths from the anterior end. the stylet is 17.1 mum long with pear-shaped knobs. males have a rounded ...199119283115
motor neurons and motor columns of the anterior spinal cord of salamanders: posthatching development and phylogenetic distribution.the posthatching development of rostral (1st-4th) spinal motor neurons was studied in ten species of salamanders, using horseradish peroxidase and cobaltic lysine tracing techniques. development of spinal motor neurons differs among species in association with differences in life history and general developmental patterns (i.e., between species with aquatic larvae versus those with direct development, with or without ontogenetic repatterning). in the plesiomorphic state, represented by species w ...19911913139
xylem sap proteins.xylem sap from apple (malus domestica borkh), peach (prunus persica batsch), and pear (pyrus communis l.) twigs was collected by means of pressure extrusion. this sap contained a number of acidic peroxidases and other proteins. two other sources of xylem sap used in this study were stem exudates and guttation fluid. similar peroxidases were also found in stem exudates and guttation fluids of strawberry (fragaria x ananassa duch.), tomato (lycopersicum esculentum l.), and cucumber (cucumis sativu ...199116668227
how does the toad's visual system discriminate different worm-like stimuli?behavioral experiments show that toads exhibit stimulus- and locus-specific habituation. different worm-like stimuli that toads can discriminate at a certain visual location form a dishabituation hierarchy. what is the neural mechanism which underlies these behaviors? this paper proposes that the toad discriminates visual objects based on temporal responses, and that discrimination is reflected in different average neuronal firing rates at some higher visual center, hypothetically anterior thala ...19912004136
[the malignant degeneration of gorham-stout disease?]. 19911764602
developmental lymphatic disorders of the thorax.developmental disorders that involve the lymphatic channels of the thorax, although rare, are important and must be distinguished from the more common causes of chest masses or diffuse lung disease. there are four major types of developmental lymphatic disorders that affect the thorax: lymphangiectasis, characterized by congenital anomalous dilatation of pulmonary lymph vessels; localized lymphangioma, a rare and benign, usually cystic, lesion characterized by masslike proliferation of lymph ves ...19911749850
ethylene production by growing and senescing pear fruit cell suspensions in response to gibberellin.a pear (pyrus communis l. cv passe crassane) cell suspension was used as a model system to study the influence of gibberellin on processes related to fruit ripening. growth of the cell cultures was inhibited and their loss of viability was accelerated when 0.5 millimolar gibberllic acid (ga(3)) was added to suspensions at two stages of cell development, namely, growth and quiescence. cell respiration rate was unaffected up to 2 millimolar ga(3) but ethylene production, both basal and 1-aminocycl ...199116668076
infrared spectroscopy of plant cell cultures : noninvasive measurement of viability.infrared spectroscopy was used to examine suspension-cultured pear (pyrus communis l.) and spartina pectinata cells. noninvasive measurements were made using internal reflectance sampling. spectra of actively growing cells exhibited a pronounced absorbance at 2343 reciprocal centimeters. the absorbance peak was identified and verified as co(2) dissolved in water. this peak was absent in nonviable cells. peak height was directly proportional to percent viability in artificial mixtures of viable a ...199116668026
[essential osteolysis. study of 3 cases and a classification trial].disappearing bone disease is a rare syndrome, characterized by an idiopathic and spontaneous progressive resorption of bone, for which different etiologies must be considered. the classification of this disease is difficult; acro-osteolysis involves the phalanges or the carpus and tarsus. massive osteolysis corresponds to diffuse multicentric cystic hemangiomatosis and gorham and stout's disease. we describe two cases of acro-osteolysis and one of gorham and stout's disease. the mechanism of dis ...19912048871
organogenesis from 'passe crassane' and 'old home' pear (pyrus communis l.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants.large numbers of highly viable mesophyll protoplasts were isolated from shoot cultures of the scion cv 'passe crassane' and the rootstock genotype 'old home' of common pear (pyrus communis l.). protoplasts were cultured for both genotypes either as liquid layers or as liquid-over-agar cultures, in ammonium-free ms medium with 0.5 m mannitol, 50 mg/l casein enzymatic hydrolysate (ceh), 2.0 mg/l naa and 1.0 mg/l bap, plus either 0.5 mg/l iaa (for 'old home') or 2.0 mg/l iaa (for 'passe crassane'). ...199224202928
some observations on the fine structure of the sensory cells in the neuromasts (ordinary lateral line) of the teleost gambusia affinis.the structural and ultrastructural features of the sensory cells in the neuromasts of g. affinis were studied. these pear-shaped cels present a tuft of sensory hairs rising from the apex and a highly dense cytoplasm with multivesicular bodies, dyctiosomes, granular and smooth endoplasmic reticulum and numerous mitochondria. large round granular dense bodies of an unknown nature can be observed inside and outside the nucleus. a great number of vesicles are present at the base of the cells, some o ...19921447513
central projections and motor nuclei of the facial, glossopharyngeal, and vagus nerves in the mormyrid fish gnathonemus petersii.most of the information about the anatomy of the fish's cranial nerves was collected in the first two decades of this century. experimental analysis of the viith, ixth, and xth cranial nerves by modern tract tracing techniques started about 20 years ago. several species have been investigated to date, including one species of agnatha (myxinoidea), two species of elasmobranchs, and species of some orders of teleostei like cyprinidae, siluriformes, perciformes, and gadidae. the sensory and motor n ...19921447406
host range of a population of pratylenchus vulnus in commercial fruit, nut, citrus, and grape rootstocks in spain.in a host-range study carried out under greenhouse conditions, a total of 37 commercial fruit tree, grape, and citrus rootstocks were tested for their reaction to a population of the lesion nematode, pratylenchus vulnus, in spain. twenty-five rootstocks had a pf/pi > 1.5. these included almond (desmayo rojo, 1143), apple (em-9, em-106), avocado (hass), cherry (santa lucia 64, camil, m x m 14, masto de montafiana), grape (41-b, fercal, ritcher 110), hazelnut (pauetet), loquat (nadal), peach (mont ...199219283047
expression of erwinia amylovora hrp genes in response to environmental stimuli.seven hrp loci that are essential for the hypersensitive reaction elicited by erwinia amylovora were transcriptionally fused with a derivative of transposon tn5, containing the promoterless escherichia coli beta-glucuronidase reporter gene. the seven hrp fusions were used to monitor expression of the hrp loci in vitro and in planta. no significant expression was detected in rich medium for any of the fusions. however, five of them were expressed highly in planta and in inducing medium that conta ...19921372313
pear blister canker viroid is a member of the apple scar skin subgroup (apscaviroids) and also has sequence homology with viroids from other subgroups.the sequence of pear blister canker viroid (pbcvd), the putative causal agent of pear blister canker (pbc) disease, has been determined. pbcvd consists of a single-stranded circular rna of 315 nucleotide residues which assumes a branched conformation when it is folded in the model of lowest free energy. pbcvd has highest sequence similarity with grapevine 1b viroid (52.4%), but also contains sequences related to regions present in viroids that belong to different subgroups, suggesting that pbcvd ...19921383394
[morphology and immunotyping of ac-43 strain of chlamydia pneumoniae isolated from a japanese child].a strain of chlamydia pneumoniae (c. pneumoniae) which had been isolated from a japanese child (ac-43) was examined morphologically and serologically using micro-immunofluorescent (micro-if) test. inclusions of ac-43 were stained by an indirect immunofluorescent method using c. pneumoniae specific monoclonal antibody. they were dense round inclusions which had been reported as a characteristic figure for c. pneumoniae. an elementary body (eb) of ac-43 was pear-shaped by electron micrograph, whic ...19921402097
gamma-aminobutyric acid immunoreactivity in the enterochromaffin cells of the rat stomach.the present immunocytochemical study revealed gamma-aminobutyric acid (gaba) immunoreactivity in the oxyntic and pyloric mucosa of the rat stomach at light- and electron-microscopic levels. gaba-immunoreactive endocrine cells were numerously seen in the lower half portion of the pyloric mucosa but rarely in the oxyntic mucosa. these cells were round or oval in shape and sometimes had a short cytoplasmic process. serotonin-immunoreactive enterochromaffin (ec) cells were also observed in the oxynt ...19921441883
refractory barriers in the sand fly phlebotomus papatasi (diptera: psychodidae) to infection with leishmania panamensis.the life cycle of leishmania panamensis in phlebotomus papatasi was studied to characterize barriers limiting parasite colonization, differentiation, migration, and attachment in an unnatural sand fly host. the insects were fed a suspension of l. panamensis-infected macrophages and human erythrocytes, and were examined up to 16 days post-infection by light and electron microscopy. histologic examination of 401 flies showed the peritrophic membrane to be the first important barrier to parasite es ...19921539756
synaptic connections of central carotid sinus afferents in the nucleus of the tractus solitarius of the rat. i. an electron microscopic study.a transganglionic transport technique was used to study the synaptic connections of the central carotid sinus afferents in the nucleus of the tractus solitarius of the rat by electron microscopy. the caudal part of the nucleus was profusely innervated. labelled fibres extended to the contralateral nucleus, and to the ipsilateral dorsal motor nucleus of the vagus nerve, nucleus ambiguus, spinal nucleus of the trigeminal nerve and the area postrema. the labelled terminals were densely packed with ...19921560250
3-oxoacyl-[acp] reductase from oilseed rape (brassica napus).3-oxoacyl-[acp] reductase (e.c. 1.1.1.100, alternatively known as beta-ketoacyl-[acp] reductase), a component of fatty acid synthetase has been purified from seeds of rape by ammonium sulphate fractionation, procion red h-e3b chromatography, fplc gel filtration and high performance hydroxyapatite chromatography. the purified enzyme appears on sds-page as a number of 20-30 kda components and has a strong tendency to exist in a dimeric form, particularly when dithiothreitol is not present to reduc ...19921562581
[inhibitory effects of balsam pear on the mutagenic activity of cyclophosphamide in vivo].inhibitory effects of balsam pear on the mutagenic activity of cyclophosphamide were studied in the cells of bone marrow of mouse in vivo. it was found that balsam pear juice itself showed no effect on the incidence of sister chromatid exchanges (sce) and of micronuclei (mn) on the cells of bone marrow. but balsam pear juice reduced the incidence of sce (from 29.1 times/cell to 13.27-28.38 times/cell) and mn (from 60.0% to 27.0%-50.0%) induced by cyclophosphamide respectively. the inhibitory eff ...19921606864
harpin, elicitor of the hypersensitive response produced by the plant pathogen erwinia amylovora.a proteinaceous elicitor of the plant defense reaction known as the hypersensitive response was isolated from erwinia amylovora, the bacterium that causes fire blight of pear, apple, and other rosaceous plants. the elicitor, named harpin, is an acidic, heat-stable, cell-envelope-associated protein with an apparent molecular weight of 44 kilodaltons. harpin caused tobacco leaf lamina to collapse and caused an increase in the ph of bathing solutions of suspension-cultured tobacco cells. the gene e ...19921621099
effects of ethanol and various alcoholic beverages on the formation of o6-methyldeoxyguanosine from concurrently administered n-nitrosomethylbenzylamine in rats: a dose-response study.consumption of alcoholic beverages has been identified as a major cause of oesophageal cancer in industrialized countries, with an exceptionally high risk associated with apple-based liquors (calvados). in the present study, we have determined the dose--activity relationship of the effects of coincident ethanol on the formation of o6-methyldeoxyguanosine (o6-medg) by the oesophageal carcinogen n-nitrosomethylbenzylamine (nmbza). male fischer 344 rats received a single intragastric dose of nmbza ...19921638683
two-dimensional crystals of membrane-bound gastric h,k-atpase.two-dimensional crystallization of membrane-bound h,k-atpase (ec 3.6.1.36) in vesicle preparations from parietal cells of hog gastric mucosa was induced by an imidazole buffer containing mg2+ and vo3- ions. a continuous reorganization of the protein molecules started within a few hours by the formation of linear arrays. at later stages confluent two-dimensional crystals were formed. electron microscopy and image processing showed that these were of a single tetragonal type. the asymmetric unit c ...19921312039
role of antibiotic production by erwinia herbicola eh252 in biological control of erwinia amylovora.erwinia herbicola eh252 is a nonpathogenic epiphytic bacterium that reduces fire blight incidence when sprayed onto apple blossoms before inoculation with erwinia amylovora, the causal agent of fire blight. eh252 was found to produce on minimal medium an antibiotic that inhibited the growth of e. amylovora. this antibiotic was inactivated by histidine but not by fe(ii), was sensitive to proteolytic enzymes, and showed a narrow host range of activity. to determine the role of this antibiotic in t ...19921314801
localization of transposon insertions in pathogenicity mutants of erwinia amylovora and their biochemical characterization.transposon tn5, on a mobilizable cole1 plasmid, on a ti plasmid derepressed for bacterial transfer, and on the bacteriophage fd genome, was used to construct pathogenicity mutants of the fire blight pathogen erwinia amylovora. eleven nonpathogenic mutants were isolated from 1600 independent mutants screened. these mutants were divided into three types: auxotrophs, exopolysaccharide (eps)-deficient mutants and a mutant of the dsp phenotype. according to their insertion sites the tn5 mutants were ...19921322951
prickly pear (opuntia sp.) pectin reverses low density lipoprotein receptor suppression induced by a hypercholesterolemic diet in guinea pigs.the effects of prickly pear pectin on plasma ldl metabolism were investigated by feeding guinea pigs either a diet containing 15 g/100 g lard and 0.25 g/100 g cholesterol (lc diet) or the lc diet in which cellulose was partially replaced (2.5 g/100 g) by prickly pear pectin (lc-p diet). the lc-p diet lowered plasma ldl cholesterol concentrations by 33% (p < 0.001). low density lipoprotein composition was modified by intake of prickly pear pectin; the relative percentages of free and esterified c ...19921333520
aberrant histoplasma capsulatum. confirmation of identity by a chemiluminescence-labeled dna probe.a cottony, light tan, filamentous fungus with pear-shaped microconidia and lacking tuberculated macroconidia was isolated from a bronchial lavage specimen. subculture on several media at 37 degrees c failed to convert the fungus to a yeast form after several weeks; attempts at in vivo conversion in mice were also unsuccessful. sera obtained several months apart showed m bands with histoplasma capsulatum (hc) antigen by immunodiffusion and an increase in complement fixation titers with mycelial a ...19938112034
case report 803: gorham's disease (gorham-stout syndrome) of scapula.in summary, a 36-year-old man presented with pain and limited motion in the shoulder. clinical examination revealed obliteration of normal scapular landmarks in the conspicuous absence of any palpable soft tissue mass. roentgenograms showed progressive osteolysis of the scapula. biopsy confirmed the diagnosis of gorham's disease. mri played a key role in defining the extent of disease involvement and in displaying the distinct soft tissue anatomy. these mri features are to our knowledge previous ...19938248825
intragenic sequences are required for cell type-specific and injury-induced expression of the rat peripherin gene.peripherin is a 57 kda type iii intermediate-filament protein that is thought to play a role in axonogenesis both during development and following nerve injury (oblinger et al., 1989; escurat et al., 1990; gorham et al., 1990; troy et al., 1990b). we have used transgenic mouse technology to define peripherin gene sequences that are necessary for cell type-specific expression and for the increase in peripherin that occurs in response to axonal injury. correct temporal and nervous system-specific ...19938254361
[reactivity of chlamydia pneumoniae strains in the ideia chlamydia test kit designed for detection of chlamydia trachomatis].the intensity of reaction in the ideia chlamydia test kit to lipopolysaccharide of seven strains of chlamydia pneumoniae was examined by using highly purified elementary bodies (ebs). the strains were divided into two groups according to whether the ebs were pear-shape or round. the group with the pear-shaped ebs consisted of tw-183, ar-39 and ar-388 strains; the group with the round ebs consisted of iol-207, kajaani-6, yk-41 and kkpn-1 strains. the number of ebs at the cutoff level of the test ...19938336010
[dendritic arborization patterns of interneurons labeled with a lectin, vicia villosa, in rat cerebral cortex: studies by intracellular injection of lucifer yellow using aldehyde-fixed slices].in order to characterize the dendritic field of a number of interneurons in the cerebral cortex, the labeling of extracellular sugar chains which define a subset of interneurons was combined with the subsequent intracellular filling of dyes in aldehyde-fixed tissue. neurons whose cell body had been outlined by a lectin, vicia villosa (vva), which recognizes terminal n-acetylgalactosamine, were intracellularly injected with a fluorescent tracer, lucifer yellow (ly), in the rat parietal cortex und ...19938337931
life cycle of leishmania major (kinetoplastida: trypanosomatidae) in the neotropical sand fly lutzomyia longipalpis (diptera: psychodidae).the development of leishmania major yakimoff & schokhor in the new world sand fly lutzomyia longipalpis (lutz & neiva) was examined by light and electron microscopy. in this unnatural host, parasites differentiated into 10 typical morphological forms, multiplied at three sites, migrated anteriorly and established in the foregut, and attached to gut surfaces. in the blood meal, amastigotes divided and transformed into two successive dividing, stumpy promastigote stages. elongate nectomonad promas ...19938360894
sensory receptor-like cells in the human foetal vomeronasal organ.the vomeronasal organ (vno) was studied in ten human foetuses, 12 to 36 weeks old. at 12 to 23 weeks, it was lined by a smooth pseudostratified epithelium, with neurone-specific enolase (nse) positive cells looking like olfactory receptors. clusters of nse-positive cells were seen in relation with the posterosuperior end of the organ and along nerve fascicles in the nasal septum. at 36 weeks, the organ was lined by a respiratory epithelium and did not show any receptor-like cells; some pear-shap ...19938369475
isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis.we isolated a fission yeast putative protein serine/threonine phosphatase gene designated ppe1+ by hybridization. the predicted amino acid sequence is similar to those of the fission yeast ppa2 (53% identity) and dis2 (39%) phosphatases, and highly similar to those of the budding yeast sit4 (72%), drosophila ppv (68%) and rabbit ppx (61%) phosphatases. antibodies against ppe1 protein identified a 37-kd polypeptide in fission yeast. a gene disruption (designated delta ppe1) caused cold-sensitive ...19938387356
isolated brush cells of the rat stomach retain their structural polarity.the brush cells (bc) are highly polarized elements occurring in epithelia of endodermal origin. they have a preferential topographical distribution in the organs in which they reside. in the stomach of the rat, bc prevail near the transitional zone separating the forestomach from the glandular stomach. thus, a method was developed to isolate and recover bc from this organ with the aim of investigating the changes they may undergo after dissociation. strips of the rat stomach were severed from th ...19938443835
demonstration of chlamydia pneumoniae in atherosclerotic lesions of coronary arteries.chlamydia pneumoniae is a human respiratory pathogen that causes acute respiratory disease and approximately 10% of community-acquired pneumonia. the infections are geographically widespread. antibody prevalence studies have shown that virtually everyone is infected with the c. pneumoniae organisms at some time and that reinfection is common. in addition to respiratory disease, seroepidemiologic studies have shown an association of this organism with coronary artery disease. c. pneumoniae was de ...19938450249
fertility awareness as a component of sexuality education. preliminary research findings with adolescents.fertility awareness refers to the observation and interpretation of cervical mucus, often called vaginal discharge. a woman's cervical-mucus pattern indicates the time of ovulation and differentiates the fertile and infertile phases of the menstrual cycle from each other. fertility awareness enables a woman to know when pregnancy can and cannot occur on a daily basis during each menstrual cycle. there has been, to date, almost no exploration of the appropriateness of fertility-awareness instruct ...19938459941
leishmania differentiation in natural and unnatural sand fly hosts.leishmania differentiation in the gut of phlebotomine sand flies was evaluated based on five light and electron microscopic studies of natural (leishmania panamensis/lutzomyia gomezi, leishmania chagasi/lutzomyia longipalpis) and unnatural (leishmania mexicana/lutzomyia abonnenci, leishmania panamensis/phlebotomus papatasi, leishmania major/lutzomyia longipalpis) life cycles. in the bloodmeal, transformation of amastigotes into stumpy promastigotes occurred before or during division. further div ...19938461893
fibroblast growth factor induces primitive streak formation in rabbit pre-implantation embryos in vitro.culturing of rabbit pre-implantation embryos was performed in ham's f10 medium supplemented with polyvinylpyrrolidone. under these culture conditions, day 6 post coitum blastocysts increased their diameter within 24 h to 80% of that of day 7 blastocysts grown in vivo. despite this substained growth, the embryonic disc remained undifferentiated with clear signs of degeneration after 24 h of culture. basic fibroblast growth factor (bfgf) was able to overcome this developmental block. after 12 h of ...19938470827
mycoplasmalike organisms (mlos) and distinguish them from other mlos.dna of 10 lines of rice yellow dwarf (ryd) mycoplasmalike organisms (mlos) from japan, the phillippines, and thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal dna of a tochigi (japan) line of ryd mlo. one chromosomal probe (ryd9) and all six extrachromosomal probes hybridized with various other mlos (sugarcane white leaf, onion yellows, cineraria witches'-broom, japanese hornwort witches'-broom, water dropwort wiches'-broom, gentian witches'-b ...19938489230
two novel protein kinase c-related genes of fission yeast are essential for cell viability and implicated in cell shape control.two novel protein kinase c (pkc)-like genes, pck1+ and pck2+ were isolated from fission yeast by pcr. both contain common domains of pkc-related molecules, but lack a putative ca(2+)-binding domain so that they may belong to the npkc group. gene disruption of pck1+ and pck2+ establishes that they share an overlapping essential function for cell viability. cells of a single pck2 deletion display severe defects in cell shape; they are irregular and sometimes pear-like instead of cylindrical. in co ...19938491190
the morphology of chlamydia pneumoniae.the morphology of a recently isolated strain of chlamydia pneumoniae, yk-41, was compared by electronmicroscopy with c. pneumoniae twar, chlamydia trachomatis l2/434/bu and chlamydia psittaci cal 10. the results showed that "pear-shaped" morphology was not typical of c. pneumoniae. basic morphological features, such as surface projections and hexagonally arrayed, regular structures in the inside layer of the outer membrane of elementary bodies, were very similar in these strains. the structure o ...19938510134
nuclear ribosomal dna as a probe for genetic variability in the japanese pear pathotype of alternaria alternata.a restriction fragment length polymorphism analysis of nuclear ribosomal rna genes (rdna) was used to measure the amount and distribution of genetic variability in populations of the japanese pear pathotype of alternaria alternata on both micro- and macrogeographical scales. a total of 322 isolates were obtained from 13 areas in aichi, gifu, and tottori prefectures in central and western japan. the restriction fragment length polymorphism analysis revealed that the pathogen populations contained ...199316349060
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