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prevalence of intestinal parasitic infection in five farms in holambra, são paulo, brazil.a parasitological survey was carried out on 222 inhabitants of five farms in holambra, located 30 km north of campinas, são paulo, brazil, on october 1992. approximately 70% of the inhabitants were found to be infected with at least one species of intestinal parasite. the positive rates of 6 helminths and 7 protozoan species detected are as follows: 5.4% ascaris lumbricoides; 8.6% trichuris trichiura; 19.8% necator americanus; 10.4% strongyloides stercoralis; 1.4% enterobius vermicularis; 0.9% h ...19957569635
enzyme-linked immunosorbent assay for detection of serum antibody to blastocystis hominis in symptomatic infections.an enzyme-linked immunosorbent assay was devised in order to search for antibodies against blastocystis hominis in infected humans. reaction proteins were obtained from washed, axenic b. hominis cells, as sonicate. sonicate was diluted to provide 17 and 34 micrograms of protein per well. dilutions of patients' sera were applied, followed by phosphatase-conjugated goat anti-human serum and phosphatase substrate. color was measured at 405 microns wavelength. immunoglobulin g antibodies to high tit ...19957876972
bacteria, viruses, yeasts and protozoans associated with diarrheal disease in singapore.labile toxin producing enterotoxic e. coli (etec) were the commonest pathogen isolated from diarrheal stools of hospitalized children (21%) and adults (26%) in singapore. salmonellas ranked a close second in children (19%). other bacterial pathogens were isolated from less than 5% of subjects. blastocystis hominis was detected in 4.3% of diarrheal stools when a simple sedimentation technique was used. cryptosporidium was not detected at all. an analysis of yeast counts in smears of diarrheal and ...19957603751
tubulovesicular elements in blastocystis hominis from the caecum of experimentally-infected rats.by transmission electron microscopy (tem), tubulovesicular elements were seen in blastocystis hominis obtained from the caecum of experimentally-infected rats. these appeared to arise from the peripheral cytoplasm and were rounded, oval or elongate in sections. it is suggested that these elements form a network for transfer of nutrients to the periphery during the process of encystation.19957797363
serum antibody detected by fluorescent antibody test in patients with symptomatic blastocystis hominis infection. 19957501905
is there any reason to continue treating blastocystis infections? 19957578717
histochemical detection of carbohydrates of blastocystis hominis.the carbohydrates of blastocystis hominis were detected by histochemical techniques using light and electron microscopy. b. hominis, fixed with various fixatives, followed by treatment with detergents, were stained with periodic acid-schiff (pas) or alcian blue (ab). intense pas reactions were observed in cells fixed with glutaraldehyde or 1/2 karnovsky fixative. the cells fixed with other fixatives showed weak or no reactions with pas staining. similar results were seen in the case of ab stain. ...19957537145
light and electron microscopic localization of lipids in blastocystis hominis.localization of the lipid of blastocystis hominis was histochemically demonstrated. b. hominis was fixed with glutaraldehyde, treated with detergents, and stained with sudan black b or nile blue. the central vacuole of b. hominis stained with sudan black b showed many black droplets with great variation in staining intensity. in nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. these results indicated the presence of neutral lipids and/or acidic ...19957544390
blastocystis hominis and traveler's diarrhea. 19957578716
elucidation of the life cycle of the intestinal protozoan blastocystis hominis.this paper elucidates the status of the different morphological forms of blastocystis and reports the existence of thin- and thick-walled cysts in b. hominis on the basis of current experimental evidence. it is suggested that the thin-walled cysts are autoinfectious, leading to multiplication of the organism in the intestinal tract. the thick-walled cysts are responsible for external transmission via the faecal-oral route. a life cycle for b. hominis is postulated on the basis of these findings.19957501648
biochemical characterisation of human isolates of blastocystis hominis.sds-page and iso-enzyme analysis of 11 human isolates of blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. this is the first iso-enzyme and the second protein analysis to indicate strain differences in b. hominis.19957707340
is blastocystis hominis a cause of diarrhea in travelers? a prospective controlled study in nepal.although the pathogenicity of blastocystis hominis has been extensively debated in the medical literature, controlled studies of the association between b. hominis and diarrhea are lacking. we conducted a case-control study among expatriates and tourists in kathmandu, nepal, in which we compared the prevalence of the organism among patients with diarrhea to that among a control group without diarrhea. b. hominis was detected in 56 (30%) of 189 patients with diarrhea, compared with 40 (36%) of 11 ...19957578767
blastocystis hominis in human immunodeficiency virus-related diarrhea.a wide variety of bacterial, viral, and parasitic pathogens can cause severe diarrhea in patients with advanced human immunodeficiency virus (hiv) infection. conflicting evidence exists as to whether blastocystis hominis should also be included among the infectious agents capable of causing hiv-related diarrhea.19958578192
practice guidelines for ordering stool ova and parasite testing in a pediatric population. the alberta children's hospital.a comprehensive utilization review was done of all stool ova parasite examinations performed at a pediatric hospital during a 3-year period from june 1, 1989 to july 1, 1992. a total of 2,652 stool specimens were surveyed from 1,532 children. forty-one percent (1,081) of the workload was from inpatients, 37% (976) was from emergency room (er) visits/other outpatients, and 22% (233) was from patients attending the gastroenterology (gi) clinic. the prevalence of enteric parasites in hospitalized c ...19957677114
[blastocystis hominis, a potentially pathogenic parasite frequently identified after a travel in tropical regions]. 19958539222
polyparasitism in two rural communities with endemic schistosoma mansoni infection in machakos district, kenya.formol-ether concentration supplemented by fresh saline smears was used to study intestinal parasites in two communities, miu and kitengei, in machakos district, kenya. these communities differed markedly in schistosome associated morbidity, in spite of similar prevalence and intensities of infection as revealed by kato examination, seven helminth and nine protozoan species were detected among 1011 samples examined. more than 60% of the subjects were infected with more than one parasite: one had ...19958544228
[blastocystis hominis: a common commensal in the colon. study of prevalence in different populations of paris].to compare the prevalence of blastocystis hominis in different population categories in paris, including immunodepressed subjects.19958545396
occurrence of giardia lamblia in children of municipal day-care centers from botucatu, são paulo state, brazil.considering that the number of day-care centers for pre-school-age children has expanded rapidly in developing countries, and that these institutions presenting conditions that facilitate the transmission of many enteric agents, a parasitological survey was carried out in three municipal day-cares from botucatu: two in the urban area (one in downtown area and the other one in the city periphery area) and the third in the rural area. three separate stool specimens were collected from 147 children ...19958731262
a comparative morphology of blastocystis hominis cysts with and without the "fibrillar layer". 19959139399
hemorrhagic proctosigmoiditis and blastocystis hominis. 19968929001
intestinal parasitism--protozoa and helminths--in primates at the barcelona zoo.the faunistic results regarding intestinal parasitism by protozoa and helminths in 21 primate species (three cebidae, thirteen cercopithecidae, one hylobatidae, one lemuridae, three pongidae) are reported. the primate species were housed in four separate galleries. six faecal samples of each host species were subjected to coprological analysis. fifteen parasite species were detected: 11 protozoa (entamoeba coli, e. chattoni, e. hartmanni, iodamoeba bütschlii, endolimax nana, giardia intestinalis ...19969210027
[blastocystis hominis infection: a case report].blastocytis hominis has long been described as a non-pathogenetic protozoan parasite until recently, when claims have been made that it could be the cause of intestinal disorders. the clinical picture of b. hominis consists of non specific abdominal pain, watery diarrhea, anorexia, vomiting and weight loss. rarely a more invasive form of the disease with rectal bleeding can occur. we describe the case of a ten year-old girl who was admitted to our hospital for diarrhea, abdominal pain and fever. ...19969091775
observations on the ultrastructure and viability of the cystic stage of blastocystis hominis from human feces.this report describes the ultrastructure and viability of cysts of blastocystis hominis from feces of infected patients. the cysts were round to ovoid, measured 2-5 microns in size, and contained a condensed cytoplasm that had vacuoles of varying sizes, four nuclei, and as many as six cristate mitochondria. the cell wall was rather electron-lucent. surprisingly, chromatoid-like structures were found in the cytoplasm and nucleus of some of the cysts. these have not previously been reported in bla ...19968738284
identification of surface coat carbohydrates in blastocystis hominis by lectin probes.the carbohydrate residues of the surface coat of 20 axenic cultures of blastocystis hominis were studied using fitc-labelled lectins (cona, wga, dba, hpa, sba, pna, ueai and lpa). the specific affinity of reactive lectins was determinated by competitive inhibition assay with specific carbohydrates or by enzymatic pre-treatment of cells. all stocks strongly bound cona and hpa; wga, ueai and lpa were partially reactive, and the remaining lectins were nonreactive. inhibition assays showed abolition ...19968818733
[protozoan infection (blastocystis hominis) concomitant with pseudomonas sp. peritonitis in continuous ambulatory peritoneal dialysis (capd)].case-report of protozoal infection (blastocystis bominis) during pseudomonas peritonitis in male patient with intestinal diverticulosis on continuous ambulatory peritoneal dialysis (capd) treatment for chronic renal failure (crf). microscopic morphology and cultural characteristics are summarized from current literature. photographic images in phase contrast from fresh-observation of faeces and peritoneal fluid are reported. although other protozoa (e.g. acanthamoeba free-living) have already be ...19968848771
human parasite finds taxonomic home. 19968602239
dna polymorphism revealed by arbitrary primers polymerase chain reaction among blastocystis strains isolated from humans, a chicken, and a reptile.dna polymorphisms of different strains of blastocystis isolated from humans, a chicken, and a reptile were examined by an arbitrary primer pcr method. two strains of blastocystis hominis isolated from humans in the usa and japan yielded nearly identical pcr products. however, one strain of b. hominis (isolated from a human in singapore) yielded quite different pcr products. blastocystis sp. isolated from a chicken yielded pcr products similar to those of the former two strains, while blastocysti ...19968720942
resistance of blastocystis hominis cysts to chlorine. 19968936976
severe blastocystis hominis in an elderly man.we describe a unique case of severe blastocystis hominis infection in an elderly man with severe dehydration, marked leukocytosis and hypoalbuminaemia after antibiotic treatment for right pneumonia. the patient recovered after treatment with metronidazole. this case presentation demonstrates the ability of b. hominis to induce severe gastrointestinal manifestations and general deterioration, particularly in light of the controversy surrounding its possible potential pathogenicity. we believe, th ...19968842998
gastrointestinal parasite infections. 19968843840
[identification of blastocystis hominis in concentrated fecal samples]. 19969044649
in vitro response of blastocystis hominis against traditional chinese medicine.this is the first inn vitro study on the activity of 20 kinds of crude extracts of traditional chinese medicine (tcm) on the intestinal parasite, blastocystis hominis using the criteria of living cell count (lcc) and living cell rate (lcr). lcc and lcr were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. lcr calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cel ...19969121165
intestinal sarcocystosis in thai laborers.to determine the prevalence of sarcocystis and other intestinal parasites in thai laborers who were going abroad for work, stool examinations of 362 asymptomatic laborers were studied. the four most frequently parasites found in stool were sarcocystis sp (23.2%), opisthorchis viverini (40.3%), hookworm (21.5%), and strongyloides stercoralis (14.1%). giardia intestinalis (5.2%), entamoeba coli (1.7%), endolimax nana (2.5%), blastocystis hominis (4.1%), echinostoma sp (3.6%), trichuris trichiura ( ...19969031398
[blastocystis hominis in a zone of northern italy]. 19968650434
colony formation of blastocystis hominis in soft agar.this is the first description of a method for growing axenized blastocystis hominis as colonies in petri dishes containing soft agar. blastocystis cells cultured in two types of agar appeared to show different colonial morphologies as well as differing colony yields. microscopic examination of the colonies revealed many amoeboid and giant cells. many cells were also shown to possess thin filament-like structures that appeared to stretch across the central vacuole.19968740557
production and characterization of murine monoclonal antibodies to blastocystis hominis.several hybridomas producing antibodies detected by enzyme-linked immunosorbent assay (elisa) were established by fusions of mouse myeloma p3.x63.ag8.u1 with spleen cells from balb/c mice immunized against an isolate of blastocystis hominis. five strongly positive hybrids (6b6, 1d5, 1e7, 4f7 and 4g11) were cloned and all were found to secrete igm monoclonal antibodies. four mabs (6b6, 1e7, 4f7 and 4g11) reacted in immunoblots with a number of b. hominis antigens (mol. wt ranging from 25,000 to 2 ...19968773525
axenic culture of reptilian blastocystis isolates in monophasic medium and speciation by karyotypic typing.the growth of axenic reptilian isolates of blastocystis in iscove's modified dulbecco's medium (imdm) was studied and the morphology of the parasite was examined by phase-contrast microscopy. the chromosomal patterns of these reptilian isolates of blastocystis were examined by pulsed-field gel electrophoresis (pfge) and compared with those of b. hominis and b. lapemi, a sea snake blastocystis. imdm with 10% horse serum supported excellent growth of the reptilian blastocystis isolates. the parasi ...19968825212
the diagnosis of blastocystis hominis cysts in human faeces.blastocystis hominis cysts in faeces can be concentrated on ficoll column. they are pleomorphic in shape having a mean diameter of 6.65 microns (s.d. = 2.32). they may or may not have a membranous layer surrounding them. the membranous layer tends to come off with pressure or dying of the specimen and corresponds to the fibrillar layer observed under the electron microscope. the ficoll concentration technique is also useful for concentrating other protozoan cysts.19968842989
blastocystis hominis revisited.blastocystis hominis is a unicellular organism found commonly in the intestinal tract of humans and many other animals. very little is known of the basic biology of the organism, and controversy surrounds its taxonomy and pathogenicity. there morphological forms (vacuolar, granular, and ameboid) have been recognized, but recent studies have revealed several additional forms (cyst, avacuolar, and multivacuolar). the biochemistry of the organism has not been studied to any extent, and organelles a ...19968894352
clonal growth of blastocystis hominis in soft agar with sodium thioglycollate.the present report describes a method for establishment of colonies of blastocystis hominis from single cells in soft agar. the percentage of colony-forming efficiency (% cfe = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. five human blastocystis isolates chosen for this study showed no apparent variation in colonial morphology. isolated colonies were also successfully grown in liquid medium, providing a ...19968897510
resistance of blastocystis hominis cysts to metronidazole.blastocystis hominis cysts concentrated from faeces were resistant to metronidazole up to a concentration of 5 mg/ml. a cysticidal drug is therefore needed for radical treatment, to avoid relapse and to prevent transmission.19968911453
freeze-fracture cytochemistry of membrane cholesterol in blastocystis hominis.membrane cholesterol in blastocystis hominis was detected by freeze-fracture methods using a polyene antibiotic, filipin. since the intramembrane particles (imp) were distributed heterogeneously on both plasma and vacuole membranes, many imp-free areas were observed. even in filipin-treated cells, filipin-cholesterol complexes were not detected in imp-free areas on the plasma membrane, whereas on the central vacuole membrane the complexes were mainly observed in imp-free regions. these results i ...19968982792
[chronic diarrheic syndrome from blastocystis hominis]. 19968983372
the occurrence of blastocystis hominis in hiv-infected patients. 19968728052
blastocystis hominis infection. 19968684227
blastocystosis or zierdt-garavelli disease. 19968692759
obligate anaerobe or not. 19968610009
prevalence of enteropathogens in stools of rural maasai children under five years of age in the maasailand region of the kenyan rift valley.stool samples were collected during august 1994 from seventy rural maasai children under the age of five years who were living in the maasailand region of the kenyan rift valley. microbiological analysis was carried out on these samples to identify which intestinal pathogens were present among the infant population of the maasai. of the samples studied 54% were pathogen positive. the most common pathogen isolated was giardia lamblia which was detected in 31% of the samples. other pathogens that ...19968625866
intestinal parasites in children and adults with and without abdominal discomfort from the kathmandu area of nepal.the present study on intestinal parasites from the kathmandu area of nepal found that in subjectively healthy children (hc) and adults (ha) the total parasite load was 28.1% and 38.8%, respectively, whereas children (adc) and adults (ada) with abdominal discomfort had a load of 62.7% and 67.8%. the prevalence of nematodes in the 4 groups was significantly higher in those with abdominal discomfort, particularly of hookworm, enterobius, and ascaris. hymenolepis nana was the most common tapeworm, a ...19968783972
phylogenetic position of blastocystis hominis that contains cytochrome-free mitochondria, inferred from the protein phylogeny of elongation factor 1 alpha. 19968813670
intestinal parasitism in san cayetano, corrientes, argentina.an epidemiologic study was conducted in san cayetano, a village in the province of corrientes, argentina, to determine the prevalence of intestinal parasitoses in children. eighty-eight households were randomly selected. stool samples were collected from 207 children (72% of the school-age population and 12% of the total village population) over a period of six consecutive days, and were subjected to microscopic examination. of the samples examined, 170 (83%) contained one or more parasites, of ...19968897723
[case report of colitis associated with blastocystis hominis infection]. 19968905973
hemorrhagic proctosigmoiditis and blastocystis hominis infection. 19968534017
[intestinal parasitosis: our series from 1992 to 1995].from 1992 to 1995 in our department 2236 researches of intestinal parasites (2194 on stool specimens, 34 on scotch tests, 8 on enterotests) were carried out on 1200 patients (703 hiv-ab negative and 497 hiv-ab positive). on the whole 387 samples (17.34%) of 203 subjects (16.92%) were found parasitized; 92 (13.08%) patients of them were hiv-ab negative and 111 (22.33%) were hiv-ab positive. we found more frequently blastocystis hominis and giardia intestinalis among hiv-ab negative subjects and c ...199612858028
blastocystis hominis as a potential cause of diarrhea in aids patients: a report of six cases in bahia, brazil.the role of blastocystis hominis as a human pathogen is controversial, although there is some evidence suggesting that it is the agent implicated in causing diarrhea in immunocompromised patients. we report 6 cases of aids patients presenting with diarrhea with no agents identified in their stools exceptb. hominis. in all cases, treatment was followed by complete recovery from symptoms and clearance of b. hominis from the patients' stools. in 2 cases, relapse of diarrhea was followed by a positi ...199711107245
[external quality assessment in coproparasitology in havana city province, cuba]an external quality assessment in coproparasitology was carried out in 77 laboratories from havana city. a questionnaire and ten plastic vials with different intestinal parasites in a small nylon bag, duly sealed, were sent to each laboratory. answers were collected during the 72 hours after delivery. results were analyzed by means of a computer program. the majority of the laboratories (70%) passed the test; the municipalities with the worst scores in the province were lisa, marianao, and haban ...199710886830
usefulness of new dna extraction procedure for pcr technique in species identification of entamoeba isolates.in this study we have developed a modified clark and diamond (1992, 1993) method using the polymerase chain reaction to amplify amoebic ribosomal rna genes that allow either specific detection of entamoeba histolytica s. str. or amoeba species identification. dna was isolated from cultured protozoa or from stool samples (cysts and/or trophozoites). cultures of xenic or axenic material (also stool samples) were treated with easy genomic dna prep or genomic dna prep plus (a&a biotechnology, poland ...19979374591
description of an improved method for blastocystis hominis culture and axenization.an improved method for blastocystis hominis culture and axenization was developed in the present study. stool samples were cultured in prereduced boeck-drbohlav nhi modified medium (with several modifications) supplemented with antibiotics (0.4% ampicillin, 0.1% streptomycin, 0.0006% amphotericin b). axenization was performed by the combination of partial purification of b. hominis by ficoll-metrizoic acid gradient and inoculation in fresh medium containing active antibiotics against remaining b ...19979000236
[a morphological study of blastocysts in lower monkeys using light microscopy].the paper shows a high infection of some species of nonhuman primates with blastocysts. lower rates of infection with this pathogen were observed in younger animals. the blastocysts isolated from macaca mulatta were microscopically analyzed. the findings indicate a great morphological similarity between the blastocysts of primates and those of man (blastocystis hominis).19979304026
blastocystis hominis among apparently healthy food handlers in jeddah, saudi arabia.a prospective study was carried out to investigate the prevalence of blastocystis hominis among random sample of apparently healthy food handlers. a total of 250 non saudi males over 21 years of age were examined. ninety (36%) had pathogenic and non pathogenic intestinal parasites. a total of 143 parasites were detected in their stool specimens. twenty were b. hominis (13.99%) while other parasites were 123 (86.01%). b. hominis was found in 20 positive cases (22.22%) with an overall rate of 8%. ...19979425825
[blastocystis hominis, intestinal pathogen]. 19979453768
cryptosporidiosis and blastocystosis in renal transplant recipients.some intestinal parasitic infections are frequently seen in renal transplant recipients. parasites such as cryptosporidium spp. and blastocystis hominis are often asymptomatic or responsible for limited infections in normals, but may cause prolonged and heavy infections with gastrointestinal complaints, mainly diarrhea, in immunocompromised patients. such infections can often not be detected by routine diagnostic procedures, but special concentration and staining methods are needed. we investiga ...19979041537
isoenzyme patterns of blastocystis hominis patient isolates derived from symptomatic and healthy carriers.isolates of blastocystis hominis derived from patients with intestinal symptoms and from healthy carriers were cultured in vitro and isoenzyme patterns of hexokinase (e.c. 2.7.1.1), phosphoglucomutase (e.c. 2.7.5.1) and glucose phosphate isomerase (e.c. 5.3.1.9) were investigated to find evidence for pathogenic and non-pathogenic subspecies of b. hominis. for this purpose we examined 2000 patients of the out-patient department of the institute for tropical medicine in tübingen. from these, we ob ...19979491103
significantly increased igg2 subclass antibody levels to blastocystis hominis in patients with irritable bowel syndrome.blastocystis hominis is a common intestinal parasite of humans in the tropics whose pathogenic role is in dispute. its presence has been reported in a variety of intestinal disorders resembling irritable bowel syndrome (ibs) such as diarrhea, anorexia, and flatulence. we have therefore investigated a possible link between ibs and blastocystosis by determining igg antibody levels to b. hominis in patients with ibs. levels of igg antibodies were significantly elevated in patients with ibs compared ...19979129532
experimental blastocystis hominis infection in laboratory mice.young (less than 8 weeks old) immunocompetent balb/c mice became infected with blastocystis hominis after inoculation of fecal cysts orally and of in vitro axenic-culture forms intracecally. this study confirmed that the fecal cyst was the form responsible for external transmission and that the mode of transmission was by the fecal-oral route. the infection was self-limiting and the infected balb/c mice appeared normal except that some of them showed weight loss and lethargy. both vacuolar and g ...19979134552
variation in the cyst morphology of blastocystis hominis.two types of blastocystis hominis cyst are passed in human feces, one with an outer fibrillar coat and the other without it. the outer fibrillar coat is shed as the cyst matures, but another fibrillar coat forms that is closely attached to the cyst wall and is clearly visible at high magnification in the electron microscope. the internal structure of the two cyst form is similar.19979089732
intestinal parasites among food-handlers in qualyobia governorate, with reference to the pathogenic parasite blastocystis hominis.a total of 1700 male food handlers, above 20 years of age who came for health clearance certificate were the subjects of the present study. health assessment questionnaire was filled out on each person including dietary habits, water supply, history of diarrhoeal disease. clinical examination and stool samples collection in 3 alternative days were performed. the food handlers were divided into symptomatic (700) and saymptomatic (1000). different concentration methods as well as test tube culture ...19979257986
observations on the surface coat of blastocystis hominis.the surface coat of blastocystis hominis was studied in the electron microscope. in some cells the surface coat was seen in two layers; the external layer was more electron-dense and fragmented than the internal layer. it appears that the surface coat is being continuously formed by the parasite and shed in the environment. the fibrillar material of the surface coat attaches to the bacteria, in some cases, completely surrounding them, possibly causing cytoplasmic damage to the bacterial cell as ...19979272568
[prevalence of blastocystis hominis infection in schoolchildren from bolivar city, venezuela].blastocystis hominis is found in about 25% of feces in normal asymptomatic people. its pathogenic role is still discussed. a prospective study was performed to determine the prevalence of b. hominis infection in schoolchildren from bolivar city. we evaluated 446 children, between five and fourteen years old, both sexes, using direct examination of feces and willis method. they were also evaluated clinically. results showed that b. hominis had a prevalence of 16.8%. we did not find a statisticall ...19979640685
parasites elicited cross-reacting antibodies to opisthorchis viverrini.two batches of crude antigens extracted from adult opisthorchis viverrini worms were compared. one was derived from adult worms harvested from the livers of laboratory infected hamsters and another was obtained from worms sedimented from the faeces of opisthorchiasis patients following treatment with praziquantel. sds-page and coomassie brilliant blue staining revealed that the two preparations had similar protein components of which the predominant ones were the 17-18 kda doublet. the antigens ...19979346276
[extraintestinal blastocystosis. a review of the literature]. 19979380947
survival of blastocystis hominis clones after exposure to a cytotoxic monoclonal antibody.our previous studies have shown that monoclonal antibodies (mabs) to blastocystis hominis react mainly with carbohydrate epitopes, while 1 mab (1d5) reacts specifically with a protein of 30.5 kda. in the present study, 3 monoclonal antibodies (1d5, 1e7 and 4f7) were used in immunogold localization. 1e7 and 4f7 were found to react primarily with the surface coat, while 1d5 was plasma membrane-specific. in the presence of complement, only 1d5 exhibited a cytotoxic effect on b. hominis whereas 1e7 ...19979292311
blastocystis hominis in animals: incidence of four serogroups.in toto, 520 faecal samples from mammals, birds, reptiles, amphibians, fish, and snails were investigated (see table 1). 91 strains of blastocystis hominis could be isolated by culture. however, only 48 of them were suitable for axenisation. 96 percent of samples belonged to four serogroups detected in humans but two strains, one from a pig and another from a duck, could not be classified, suggesting the existence of one or two further serogroups. while humans showed mainly serogroups i and ii, ...19979361389
[parasitosis in an adult population with chronic gastrointestinal disorders].we worked with 185 middle-class patients above 18 years of age, both sexes, who presented diarrhea and/or chronic gastrointestinal disorders. the faeces were collected serially in formol 10% and processed in the following way: direct microscopy, with and without wet staining, concentration by ritchie's method, 1% safranine technique for a specific investigation of cryptosporidium sp., and faecal sieving macroparasites. twenty eight point six of the studied patients showed at least one enteropara ...19979412130
[human infection by intestinal protozoa and helminths in calbuco county, x region, chile, 1997].by the performance of parasitological examination of one fecal sample per individual, a total of 256 persons from a rural county in the x region (41 degrees 50 minutes south lat., 73 degrees 05 minutes west long.) were studied. the general rates of infection by intestinal parasite and/or commensal protozoa and helminths found were: giardia intestinalis 14.1%, entamoeba histolytica 11.7%, blastocystis hominis 36.0%, entamoeba coli 9.8%, endolimax nana 16.4%, iodamoeba buetschlii 1.2%, chilomastix ...19979497539
nitazoxanide for the treatment of intestinal protozoan and helminthic infections in mexico.a study to evaluate the efficacy and safety of nitazoxanide as a single agent for the treatment of a broad spectrum of mixed parasitic infections, both protozoa and helminths, was conducted at a primary school in san pedro tolimán, querétaro, mexico. three faecal samples from 1824 adults and children were screened for the presence of oocysts, cysts, trophozoites, eggs or larvae of intestinal protozoa or helminths. two hundred and forty-six adults and children infected with at least one protozoan ...19979580117
epidemiologic survey of blastocystis hominis infection in japan.the incidence of blastocystis hominis in a healthy population was determined by fecal examination of 6,422 japanese and 54 resident non-japanese who visited the st. luke's international hospital health screening center for a routine medical check-up during a one-year period. of the enrolled subjects, 30 japanese (0.5%) and four non-japanese (7.4%) had b. hominis in their stools. these individuals were asymptomatic except for one who reported flatus and one who reported mild abdominal discomfort. ...19979158042
karyotypic diversity among blastocystis hominis isolates.fifteen blastocystis hominis strains, 13 axenic and 2 monoxenic, have been included in the present study. the chromosomal pattern was analyzed by the contour-clamped homogeneous electric-field (chef) system. the number of chromosomes detected ranged between 9 and 13, with sizes from 2200 kbp to 260 kbp. eleven karyotypic profiles, with a common pattern constituted by 8 chromosomes of 2200, 1280, 890, 840, 700, 650, 540 and 260 kbp, were observed. the jaccard index demonstrated that the similarit ...19979292310
enteropathogens associated with diarrhea among military personnel during operation bright star 96, in alexandria, egypt.this study investigated the microbial causes of diarrheal disease among u.s. troops deployed near alexandria, egypt, during october 1995. bacterial causes associated with 19 cases of diarrhea included: enterotoxigenic escherichia coli (etec), 42% (21% heat-stable, 11% heat-labile, and 11% heat-stable/ heat-labile producers); enteropathogenic e. coli (5.3%); and enteroadherent e. coli (42%). four cases of diarrhea were associated with enteroaggregative e. coli based on probe analysis for enteroag ...19979183160
[routine cases in intestinal parasitology].the majority of intestinal parasites isolated in switzerland is imported by refugees and travellers. nematodes are more frequently isolated in the first group, whereas giardia duodenalis and strongyloides stercoralis predominate in the second. symptoms are usually mild, but occasionally diarrhea, abdominal pain and weight loss may be encountered. hypereosinophilia occurs frequently with some parasites. four cases chosen from the outpatient department of a swiss medical polyclinic are presented a ...19979190665
five subgroups of blastocystis hominis from symptomatic and asymptomatic patients revealed by restriction site analysis of pcr-amplified 16s-like rdna.dna polymorphism of blastocystis hominis isolates was examined by the amplification of a gene fragment coding for the 16s-like rdna. using identical primers, fragments of approximately 850 bp were amplified from 110 b. hominis isolates and fragments of 1.1 kbp were amplified from 48 isolates. digestion of the amplification products with the restriction enzymes hinfi, rsai, and alui revealed different profiles for each fragment length. subgroup i and ii, resulting from digestion of the smaller 85 ...19979294547
extensive genetic diversity in blastocystis hominis.blastocystis homonis is a common human parasite of uncertain role in human disease. approximately equal numbers of reports implicate it and exonerate it as a pathogen. genetic diversity in b. hominis was investigated using riboprinting to study sequence variation in the small subunit ribosomal rna genes of 30 randomly selected isolates. extensive sequence variation was discovered in b. hominis ribosomal rna genes and this species consists of at least seven morphologically identical but genetical ...19979233675
[clinical significance of blastocystis hominis infection: epidemiologic study].to evaluate the frequency of blastocystis hominis parasitation and to ascertain its role as an intestinal a prospective study during 18 months pathogen has been carried out.19979303956
phase-contrast microscopy of cell division in blastocystis hominis. 19979307666
the role of blastocystis hominis as a possible intestinal pathogen in travellers.the role of blastocystis hominis as a pathogen for man has been controversially discussed, while travel history has been implicated as a risk factor of infection. few controlled studies of the association between b. hominis and symptomatic diseases have been performed. therefore, a case-control study among 795 german tourists returning from tropical countries was conducted. the prevalence of the organism among patients with and without symptoms was assessed. blastocystis hominis was detected in ...19979279726
parasitological and nutritional situation of school children in the sukaraja district, west java, indonesia.a parasitological survey of children aged 8 to 10 years from ten schools located in the rural district sukaraja, west java, indonesia was conducted in december 1995. a total of 348 fecal samples were examined by using modified kato-katz thick smear techniques, 365 blood samples for the measurement of hemoglobin concentration, and anthropometric data were obtained from 404 participants. four nematode (hookworm taken as one species), two cestode and nine protozoan species were detected, but no tre ...19979322303
blastocystis hominis infection in cuban aids patients. 19979332594
evaluation of intestinal protozoan morphology in human fecal specimens preserved in ecofix: comparison of wheatley's trichrome stain and ecostain.as a result of disposal problems related to the use of mercury compounds, many laboratories have switched from mercuric chloride-based schaudinn's and polyvinyl alcohol (pva) stool preservatives to other, non-mercury-based preservatives. a comparison of organism recoveries and morphologies of the intestinal protozoa was undertaken with pva containing the ecofix zinc-based schaudinn's preservative (meridian diagnostics, inc.); both wheatley's modification of gomori's trichrome stain (wt) and ecos ...19989650947
scanning electron microscopy of blastocystis hominis cysts.scanning electron microscopy of blastocystis hominis cysts reveals that some cysts have an outer coat, whereas others are naked. if intact, the outer coat forms a fan-like structure around the cyst and its surface is granular. the fragmented outer coat adheres to other cysts and bacteria, forming irregular clumps.19989660137
cytopathic effects of blastocystis hominis on chinese hamster ovary (cho) and adeno carcinoma ht29 cell cultures.blastocystis hominis isolates from asymptomatic carriers and symptomatic patients were cultured in vitro, purified from the co-cultivated bacterial flora and tested for cytopathic effects on monolayers of chinese hamster ovary (cho) cells and adeno carcinoma ht29 cells. in the case of the cho cells, living b. hominis cells and b. hominis cell lysates were able to cause significant cytopathic effects, which were dependent on the concentration of cells employed. destruction of the cell monolayers ...19989623944
the differential identification of blastocystis hominis cysts. 19989625921
[an educational intervention to improve the quality of coproparasitological diagnosis in laboratories of havana city, cuba]the aim of this study was to evaluate the effectiveness of an educational program carried out in 1993. the intervention took place after the first external quality assessment in coproparasitology, conducted in 77 laboratories of havana city. centers receiving training were compared with those that did not, and better results were obtained in the municipalities of 10 de octubre, plaza, and cerro, as well as in all laboratories that sent people to training. better diagnosis was found for the helmi ...19989592219
glycerol with fetal calf serum--a better cryoprotectant for blastocystis hominis. 19989569099
hep-2 cell-adherent escherichia coli and intestinal secretory immune response to human immunodeficiency virus (hiv) in outpatients with hiv-associated diarrhea.hep-2 cell-adherent escherichia coli and the human immunodeficiency virus (hiv) itself have recently been incriminated as causes of chronic hiv-associated diarrhea. this study sought to determine the prevalence of these two agents among hiv-infected patients with diarrhea in an outpatient setting in the united states and to compare their prevalence to that of other commonly recognized enteropathogens known to be present in this population. hep-2 cell-adherent e. coli was found in 20 of 83 (24.1% ...19989455887
in vitro encystation of blastocystis hominis: a kinetics and cytochemistry study.the kinetics of in vitro encystation of blastocystis hominis was studied over 9 days. the differentiation between trophic (tf) and cyst forms (cf) was determined by differential counts before and after treatment with distilled water. a cytochemistry study using acridine orange and calcofluor white wet-mount preparations of cf was carried out. the growth curves of tf and cf were related because the decrease in tf was followed by an increase in cf, and vice versa. the maximum of cf counts was obta ...19989491427
etiologies of acute, persistent, and dysenteric diarrheas in adults in bangui, central african republic, in relation to human immunodeficiency virus serostatus.a study of the etiologies of diarrhea in adults in relation to their human immunodeficiency virus (hiv) serostatus and number of cd4+ cells was carried out in the central african republic. in cases and controls, multi-parasitism was observed. salmonella spp. were identified mainly during acute diarrhea, with 50% of the s. enteritidis isolated during the study being responsible for septicemia and/or urinary tract infection in immunodeficient patients. enteroaggregative escherichia coli (eaggec) w ...19989886215
[health examination of children from the democratic sahara republic (north west africa) on vacation in spain].we present results of a global health evaluation of groups of children from the democratic sahara republic who came to spain for vacation.19989718764
[blastocystis hominis: the enigma continues]. 19989789449
intestinal parasite infections after extended use of chloroquine or primaquine for malaria prevention.comparative results of baseline and endpoint screening for intestinal parasites are reported from javanese men enrolled in a year-long, placebo-controlled malaria prophylaxis trial in irian jaya. the objective was to detect nontarget qualitative changes that may have resulted from prolonged chloroquine (300 mg base weekly) or primaquine (0.5 mg base/kg daily) prophylaxis. fresh fecal specimens were examined (blinded trial) for parasites and ova using a modified kato-katz thick smear method. more ...19989645872
[the clinical manifestations produced by blastocystis hominis in childhood]. 19989611725
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