Publications

identification of multiple phytotoxins produced by fusarium virguliforme including a phytotoxic effector (fvnis1) associated with sudden death syndrome foliar symptoms.	sudden death syndrome (sds) of soybean is caused by a soilborne pathogen, fusarium virguliforme. phytotoxins produced by f. virguliforme are translocated from infected roots to leaves, in which they cause sds foliar symptoms. in this study, additional putative phytotoxins of f. virguliforme were identified, including three secondary metabolites and 11 effectors. while citrinin, fusaric acid, and radicicol induced foliar chlorosis and wilting, soybean mosaic virus (smv)-mediated overexpression of ...	2016	26646532
development of virus-induced gene expression and silencing vector derived from grapevine algerian latent virus.	grapevine algerian latent virus (galv) is a member of the genus tombusvirus in the tombusviridae and infects not only woody perennial grapevine plant but also herbaceous nicotiana benthamiana plant. in this study, we developed galv-based gene expression and virus-induced gene silencing (vigs) vectors in n. benthamiana. the galv coat protein deletion vector, pgmg, was applied to express the reporter gene, green fluorescence protein (gfp), but the expression of gfp was not detected due to the necr ...	2016	27493613
molecular soybean-pathogen interactions.	soybean hosts a wide variety of pathogens that cause significant yield losses. the importance of soybean as a major oilseed crop has led to research focused on its interactions with pathogens, such as soybean mosaic virus, pseudomonas syringae, phytophthora sojae, phakopsora pachyrhizi, and heterodera glycines. pioneering work on soybean's interactions with these organisms, which represent the five major pathogen groups (viruses, bacteria, oomycetes, fungi, and nematodes), has contributed to our ...	2016	27359370
soybean golgi snare 12 protein interacts with soybean mosaic virus encoded p3n-pipo protein.	soybean mosaic virus (smv), a member of the potyvirus genus, is one of the most prevalent and devastating viral pathogens in soybean-growing regions worldwide. it is generally accepted that symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. p3n-pipo, as a trans-frame protein consisting of the amino-terminal half of p3 fused to pipo of the potyvirus, plays a key role of viral cell-to-cell movement. this study provides evidence th ...	2016	27553272
the potyviral p3 protein targets eukaryotic elongation factor 1a to promote the unfolded protein response and viral pathogenesis.	the biochemical function of the potyviral p3 protein is not known, although it is known to regulate virus replication, movement, and pathogenesis. we show that p3, the putative virulence determinant of soybean mosaic virus (smv), targets a component of the translation elongation complex in soybean. eukaryotic elongation factor 1a (eef1a), a well-known host factor in viral pathogenesis, is essential for smv virulence and the associated unfolded protein response (upr). silencing gmef1a inhibits ac ...	2016	27356973
the current status of the soybean-soybean mosaic virus (smv) pathosystem.	soybean mosaic virus (smv) is one of the most devastating pathogens that cost huge economic losses in soybean production worldwide. due to the duplicated genome, clustered and highly homologous nature of r genes, as well as recalcitrant to transformation, soybean disease resistance studies is largely lagging compared with other diploid crops. in this review, we focus on the major advances that have been made in identifying both the virulence/avirulence factors of smv and mapping of smv resistant ...	2016	27965641
complete nucleotide sequences of seven soybean mosaic viruses (smv), isolated from wild soybeans (glycine soja) in china.	soybean mosaic virus (smv) is a devastating plant virus classified in the family potyviridae, and known to infect cultivated soybeans (glycine max). in this study, seven new smvs were isolated from wild soybean samples and analyzed by whole-genome sequencing. an updated smv phylogeny was built with the seven new and 83 known smv genomic sequences. results showed that three northeastern smv isolates were distributed in clade iii and iv, while four southern smvs were grouped together in clade ii a ...	2017	27909932
inheritance, fine-mapping, and candidate gene analyses of resistance to soybean mosaic virus strain sc5 in soybean.	soybean mosaic virus (smv) is one of the most devastating pathogens for soybeans in china. among the country-wide 22 strains, sc5 dominates in huang-huai and changjiang valleys. for controlling its damage, the resistance gene was searched through mendelian inheritance study, gene fine-mapping, and candidate gene analysis combined with qrt-pcr (quantitative real-time polymerase chain reaction) analysis. the parents f1, f2, and rils (recombinant inbred lines) of the cross kefeng-1 (resistance, r) ...	2017	28352959
characterization of a soybean mosaic virus variant causing different diseases in glycine max and nicotiana benthamiana.	we discovered a soybean mosaic virus (smv) variant (4278-1) that caused systemic infections in nicotiana benthamiana plants, resulting in stem stunting and leaf shriveling. the virus had a particle morphology and incubation period similar to those of other smv isolates but differed from them in the leaf symptoms it caused when infecting soybean and n. benthamiana. the genome of this variant consisted of a 9994-nt single-stranded rna, which was different from most of the other known smv isolates ...	2017	27804020
deep sequencing leads to the identification of eukaryotic translation initiation factor 5a as a key element in rsv1-mediated lethal systemic hypersensitive response to soybean mosaic virus infection in soybean.	rsv1, a single dominant resistance locus in soybean, confers extreme resistance to the majority of soybean mosaic virus (smv) strains, but is susceptible to the g7 strain. in rsv1-genotype soybean, g7 infection provokes a lethal systemic hypersensitive response (lshr), a delayed host defence response. the rsv1-mediated lshr signalling pathway remains largely unknown. in this study, we employed a genome-wide investigation to gain an insight into the molecular interplay between smv g7 and rsv1-gen ...	2017	27019403
robust rnai-mediated resistance to infection of seven potyvirids in soybean expressing an intron hairpin nib rna.	viral pathogens, such as soybean mosaic virus (smv), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. engineering resistance by rnai-mediated gene silencing is a powerful strategy for controlling viral diseases. in this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, nib) gene was isolated from the smv sc3 strain, driven by the leaf-specific rbcs2 promoter from phaseolus vulgaris, and introduced into soybean. th ...	2017	28840434
rnai-mediated smv p3 cistron silencing confers significantly enhanced resistance to multiple potyvirus strains and isolates in transgenic soybean.	robust rnai-mediated resistance to multiple potyvirus strains and isolates, but not to secovirus bpmv, was conferred by expressing a short smv p3 hairpin in soybean plants. engineering resistance to multiple potyvirus strains is of great interest because of a wide variability of the virus strains, and mixed infections of multiple viruses or strains commonly associated with field grown soybean. in this study, rnai-mediated silencing of the soybean mosaic virus (smv) p3 cistron, which is reported ...	2017	28756582
fine-mapping and identification of a novel locus rsc15 underlying soybean resistance to soybean mosaic virus.	rsc15, a novel locus underlying soybean resistance to smv, was fine mapped to a 95-kb region on chromosome 6. the rsc15- mediated resistance is likely attributed to the gene gmpex14 , the relative expression of which was highly correlated with the accumulation of h 2 o 2 along with the activities of pod and cat during the early stages of smv infection in rn-9. soybean mosaic virus (smv) causes severe yield losses and seed quality deterioration in soybean [glycine max (l.) merr.] worldwide. a ser ...	2017	28825113
downregulation of genes coding for core rnai components and disease resistance proteins via corresponding micrornas might be correlated with successful smv infection in soybean.	plants protect themselves from virus infections by several different defense mechanisms. rna interference (rnai) is one prominent antiviral mechanism, which requires the participation of ago (argonaute) and dicer/dcl (dicer-like) proteins. effector-triggered immunity (eti) is an antiviral mechanism mediated by resistance (r) genes, most of which encode nbs-lrr family proteins. micrornas (mirnas) play important regulatory roles in plants, including regulation of host defenses. smv (soybean mosaic ...	2017	28695996
over-expression of gmsn1 enhances virus resistance in arabidopsis and soybean.	gmsn1 enhances virus resistance in plants most likely by affecting the expression of signal transduction and immune response genes. soybean mosaic virus (smv) infection causes severe symptom and leads to massive yield loss in soybean (glycine max). by comparative analyzing gene expression in the smv-resistant soybean cultivar rsmv1 and the susceptible cultivar ssmv1 at a transcriptome level, we found that a subgroup of gibberellic acid stimulated transcript (gast) genes were down-regulated in sm ...	2017	28656325