Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| detection of feline immunodeficiency virus (fiv) nucleic acids in fiv-seronegative cats. | a study was undertaken to determine the rate of viral transmission among naive specific-pathogen-free (spf) cats living in close contact with feline immunodeficiency virus (fiv)-infected cats. twenty spf cats were housed in the same rooms with experimentally fiv-infected seropositive and virus culture-positive cats for 2 to 4 years and were monitored for the presence of fiv nucleic acids and antibodies. only 1 of the 20 cats became seropositive and virus culture positive and developed signs of d ... | 1992 | 1318395 |
| a survey of fiv antibodies and felv antigens in free-roaming cats in the capital area of finland. | feline immunodeficiency virus (fiv) was first isolated and identified in 1986. since then it has been shown to have a worldwide distribution, and the infection generally appears to have reached a state of endemicity. this is the 1st study of fiv-prevalence in finland. serum samples of 196 free-roaming cats were tested for antibodies to fiv and felv antigens (feline leukemia virus). with a combined enzyme-linked immunosorbent assay (elisa), 13 of the cats (6.6%) turned out to be positive for fiv ... | 1992 | 1317991 |
| prevalence of feline immunodeficiency virus and other retroviral infections in sick cats in italy. | two hundred and seventy-seven sick pet cats living in italy were tested for antibodies to feline immunodeficiency virus (fiv) and for feline leukemia virus (felv) antigen. overall, 24% of the cats resulted positive for anti-fiv antibody and 18% for felv antigen. fiv was isolated from the peripheral mononuclear blood cells of ten out of 15 seropositive cats examined and from one out of eight saliva samples. no fiv isolations were obtained from six serum samples cultured. feline syncytium forming ... | 1992 | 1317073 |
| prevalence of feline leukemia virus and antibodies to feline immunodeficiency virus in cats in norway. | serum samples from 224 norwegian cats were analyzed for the presence of feline leukemia virus (felv) p27 common core antigen, and for antibodies to feline immunodeficiency virus (fiv). ninety specimens originated from the serum bank at the central referral clinic at the norwegian college of veterinary medicine, which had been collected during the years 1983-1989; 67 sera were submitted from veterinarian practitioners; while 67 sera originated from cats presented for euthanasia. the cats were cla ... | 1992 | 1316024 |
| toxoplasma gondii: an aids enhancing cofactor. | disseminated toxoplasmosis, one of the most severe acquired immune deficiency syndrome (aids)-associated infections in humans, is believed to develop from a latent infection after the cellular immune system is suppressed by human immunodeficiency virus type 1 (hiv-1). however, toxoplasma gondii may serve as a cofactor in enhancing the immunodeficiency induced by hiv-1. this hypothesis is supported by the facts that: 1) co-infection with other pathogens in humans infected with hiv-1 may enhance t ... | 1992 | 1334211 |
| cell-specific envelope glycosylation distinguishes fiv glycoproteins produced in cytopathically and noncytopathically infected cells. | feline immunodeficiency virus (fiv) infection induces syncytium formation and cell death in primary feline astrocyte cultures but persistently and noncytopathically infects crandell feline kidney cells (crfk). because viral envelope glycoproteins are implicated in cell fusion events we evaluated the astrocyte-produced fiv surface glycoprotein for properties that might distinguish it from that produced in crfk cells. the surface glycoprotein from astrocytes migrated faster on sds-page and contain ... | 1992 | 1314453 |
| comparison of the viral promoter activities in feline cell lines (crfk and fcwf-4 cells). | 1992 | 1313705 | |
| further characterization of a feline t-lymphoblastoid cell line (mya-1 cells) highly sensitive for feline immunodeficiency virus. | 1992 | 1313703 | |
| detection of feline immunodeficiency proviral dna in peripheral blood lymphocytes by the polymerase chain reaction. | feline immunodeficiency virus (fiv) proviral dna was detected by the polymerase chain reaction method (pcr). pcr products were detected by gel electrophoresis and ethidium bromide staining. the p-10, p-15 and p-24 regions of the gag gene of fiv were chosen as the target sequences for amplification, and three primer pairs were prepared. the pcr products subjected to amplification with each primer pair were found to possess sites of digestion by a restriction enzyme, as hypothesized. they did not ... | 1992 | 1313618 |
| molecular characterization and heterogeneity of feline immunodeficiency virus isolates. | we have molecularly cloned the complete genomic dna of tm2 strain of feline immunodeficiency virus (fiv) isolated in japan and compared its nucleotide and the deduced amino acid sequence with those of previously described u.s. isolates, fiv petaluma and fiv ppr. the infectious molecular clone of fiv tm2 is different from fiv petaluma in host cell range; the clone can not infect crandell feline kidney cells which were permissive for fiv petaluma. the amino acid sequence homologies, in gag, pol, a ... | 1992 | 1312825 |
| distinct subsets of retroviruses encode dutpase. | the nonprimate lentiviruses feline immunodeficiency virus, equine infectious anemia virus, visna virus, and caprine encephalitis virus contain a gene segment in the polymerase gene that is lacking in the primate lentiviruses. a related sequence has been noted in other retroviruses, most notably the type d retroviruses. computer searches have indicated a relatedness between this unique gene segment, termed proteaselike element and elements of both the aspartate proteinase and the dutpase enzyme f ... | 1992 | 1310783 |
| decline in cd4+ cell numbers in cats with naturally acquired feline immunodeficiency virus infection. | t-cell subsets were studied by fluorescence-activated cell sorter analysis in 57 feline immunodeficiency virus (fiv)-seropositive cats with naturally acquired fiv infection to see whether cd4(+)-cd8+ alterations were comparable to those observed in human immunodeficiency virus-infected patients. cd4+ values were decreased and cd8+ values were increased. the cd4+/cd8+ ratio was reduced to 1.6, compared with 3.3 in 33 fiv-seronegative control cats. variance analysis of data showed a significant in ... | 1992 | 1310760 |
| regulation of gene expression directed by the long terminal repeat of the feline immunodeficiency virus. | the long terminal repeat (ltr) of a retrovirus contains sequence elements that constitute a promoter for controlling viral gene expression in infected cells. we have examined regulation of ltr-directed gene expression in feline immunodeficiency virus (fiv), a t-lymphocytopathic lentivirus associated with a fatal aids-like disease in domestic cats. two independent virus isolates, designated fiv-petaluma and fiv-ppr, have been molecularly cloned and show greater than 85% sequence homology. both cl ... | 1992 | 1310554 |
| identification and analysis of the gag-pol ribosomal frameshift site of feline immunodeficiency virus. | the pol genes of retroviruses are translated as gag-pol fusion proteins by ribosomal frameshifting within the gag-pol overlap region. during the ribosomal frameshift event, the gag open reading frame is shifted -1 nt to allow in-phase reading of the pol open reading frame. a consensus frameshift signal sequence of gggaaac within the gag-pol overlap region of feline immunodeficiency virus (fiv) has been identified followed by a sequence that has the potential for a pseudoknot tertiary structure. ... | 1992 | 1310175 |
| expression of feline immunodeficiency virus gag gene in escherichia coli. | the gag gene of a japanese feline immunodeficiency virus (fiv) isolate, designated as fiv tm 2, was expressed in escherichia coli as a fusion protein with trpe. using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to fiv gag protein in feline sera. with serum samples from a cat experimentally infected with fiv, it was demonstrated that the period of seroconversion detected by this method corresponded to that by western blotting. | 1992 | 1309999 |
| retrovirus infections in non-domestic felids: serological studies and attempts to isolate a lentivirus. | an african lioness from the zoo of zurich had to be euthanized because of an inoperable tumor. the serum tested negative for feline leukemia virus (felv) p27 antigen by enzyme-linked immunosorbent assay (elisa) but was strongly positive for feline immunodeficiency virus (fiv) antibodies by elisa and western blot. when her only offspring and mate were tested for fiv, high antibody titers to fiv were also found in their serum. lymphocytes were prepared from these two lions on different occasions a ... | 1992 | 1337398 |
| feline immunodeficiency virus neurotropism: evidence that astrocytes and microglia are the primary target cells. | to investigate the neuropathogenesis of feline immunodeficiency virus (fiv) infection in vitro, we have utilized three populations of cultured feline neural cells (astrocytes, microglia, brain endothelium) to assess the relative susceptibility to fiv infection, ability to produce viral antigens, and effects of infection on cell survival. astrocytes appeared to be the most susceptible to infection, followed by microglia, whereas brain endothelial cells were relatively resistant to infection. astr ... | 1992 | 1337399 |
| isolation and partial characterization of infectious molecular clones of feline immunodeficiency virus obtained directly from bone marrow dna of a naturally infected cat. | replication-competent molecular clones of feline immunodeficiency virus (fiv) were isolated directly from the dna of bone marrow cells of a naturally fiv-infected cat. after transfection in a feline kidney cell line (crfk) and subsequent cocultivation with peripheral blood mononuclear cells (pbmc), the viral progeny of the clones was infectious for pbmc but not for crfk cells. pbmc infected with these clones showed syncytium formation, a decrease in cell viability, and gradual loss of cd4+ cells ... | 1992 | 1309891 |
| selective inhibition of human immunodeficiency viruses by racemates and enantiomers of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]cytosine. | 2',3'-dideoxy-5-fluoro-3'-thiacytidine (ftc) has been shown to be a potent and selective compound against human immunodeficiency virus type 1 in acutely infected primary human lymphocytes. ftc is also active against human immunodeficiency virus type 2, simian immunodeficiency virus, and feline immunodeficiency virus in various cell culture systems, including human monocytes. the antiviral activity can be prevented by 2'-deoxycytidine, but not by other natural nucleosides, suggesting that ftc mus ... | 1992 | 1283296 |
| feline immunodeficiency virus and tests for hiv. | 1992 | 1313615 | |
| induction of feline immunodeficiency virus-specific cytolytic t-cell responses from experimentally infected cats. | we have examined the in vitro induction and activity of feline immunodeficiency virus (fiv)-specific cytolytic t cells obtained from cats experimentally infected for 7 to 17 weeks or 20 to 22 months with the petaluma isolate of fiv. normal or fiv-infected autologous and allogeneic t lymphoblastoid cells were used as target cells in chromium-51 or indium-111 release assays. when effector cells consisted of either fresh peripheral blood mononuclear cells or concanavalin a- and interleukin-2-stimul ... | 1992 | 1323704 |
| analysis of the amino terminal presequence of the feline immunodeficiency virus glycoprotein: effect of deletions on the intracellular transport of gp95. | the envelope glycoprotein of feline immunodeficiency virus (fiv) consists of two noncovalently associated subunits, the surface glycoprotein (su; gp95) and the transmembrane glycoprotein (tm; gp40). an unusual feature of the open reading frame (orf) encoding the fiv glycoprotein is the presence of an unusually long amino terminal sequence (149 amino acids, "l" region or n-region of the signal sequence) preceding the predicted hydrophobic signal sequence. to examine the role of this n-region in t ... | 1992 | 1325696 |
| transmission of feline immunodeficiency virus from infected queens to kittens. | this study demonstrates the transmission of feline immunodeficiency virus (fiv) from infected queens to kittens in two separate litters. queen 1 was infected by intravenous administration of fiv at 22 days prior to parturition. two out of three kittens from the litter were found to be viremic at 10 weeks of age as detected by culture isolation and polymerase chain reaction detection of fiv dna in peripheral blood mononuclear leukocytes. the third kitten remained aviremic through 40 weeks of age. ... | 1992 | 1337404 |
| diseases of the european wildcat (felis silvestris schreber, 1777) in great britain. | the author describes an examination conducted in collaboration with the nature conservancy council of great britain into the status with regard to disease, conservation and genetics of the european wildcat (felis silvestris). feline leukaemia virus (felv) infection was detected by positive enzyme-linked immunosorbent assay in blood from 2 of 23 wildcats and was tested and confirmed by felv isolation in one of the two cats. this is the first time the virus has been clearly demonstrated in a free- ... | 1992 | 1339067 |
| immunological changes in cats with concurrent toxoplasma gondii and feline immunodeficiency virus infections. | to examine the immunological changes in cats concurrently infected with feline immunodeficiency virus (fiv) and toxoplasma gondii, kittens (four per group) were inoculated with fiv, t. gondii, both agents, or no pathogens. blood mononuclear cells and plasma were collected weekly for lymphocyte assays and serology. at week 14, spleen and lymph node cells were used for lymphocyte assays; brains and mesenteric lymph nodes were used for isolation of t. gondii. more t. gondii organisms were present i ... | 1992 | 1346403 |
| identification of the rev transactivation and rev-responsive elements of feline immunodeficiency virus. | spliced messages encoded by two distinct strains of feline immunodeficiency virus (fiv) were identified. two of the cdna clones represented mrnas with bicistronic capacity. the first coding exon contained a short open reading frame (orf) of unknown function, designated orf 2. after a translational stop, this exon contained the l region of the env orf. the l region resides 5' to the predicted leader sequence of env. the second coding exon contained the h orf, which began 3' to env and extended in ... | 1992 | 1323707 |
| incidence of feline immunodeficiency virus reactive antibodies in free-ranging lions of the kruger national park and the etosha national park in southern africa detected by recombinant fiv p24 antigen. | lion sera from the kruger national park (knp) dating back to 1977 and from the etosha national park (enp), obtained from 1989 to 1991, have been analysed by elisa and western blot analyses using a genetically engineered antigen representing the p24 structural protein of feline immunodeficiency virus (fiv). it was concluded that some 83% of 98 knp lion sera reacted with the p24 antigen, while none of 28 enp lion sera reacted. a few other knp felids (cheetahs and genets) gave samples that did not ... | 1992 | 1338477 |
| effect of primary phase feline immunodeficiency virus infection on cats with chronic toxoplasmosis. | the effect of primary phase feline immunodeficiency virus (fiv) infection on clinical signs, hematological values, toxoplasma gondii oocyst shedding, t. gondii-specific serology, t. gondii-specific cell-mediated immune responses, non-specific cell-mediated immune responses, and lymphocyte subpopulations from cats with experimentally induced chronic toxoplasmosis was studied. no significant clinical or hematologic abnormalities were noted following inoculation with fiv. t. gondii-specific igm was ... | 1992 | 1363007 |
| use of two virustatica (azt, pmea) in the treatment of fiv and of felv seropositive cats with clinical symptoms. | in the present study the therapeutic efficacy and the side effects of two antiretroviral compounds used in human acquired immunodeficiency syndrome (aids) research, 3'-azido-2',3'-dideoxythymidine (azt, zidovudine, retrovir) and 9-(2-phosphonylmethoxyethyl)adenine (pmea), were investigated in the treatment of cats naturally infected with feline immunodeficiency virus (fiv) and cats naturally infected with feline leukemia virus (felv). azt was administered subcutaneously at a dose of 5 mg kg-1 bo ... | 1992 | 1363008 |
| major core proteins, p24s, of human, simian, and feline immunodeficiency viruses are partly expressed on the surface of the virus-infected cells. | we have previously shown the expression of human immunodeficiency virus type 1 (hiv-1) major gag protein, p24, on the surface of persistently hiv-1-infected cells by using murine monoclonal antibodies (mab). we now report that the cell surface gag p24 antigen expression is a universal phenomenon among hiv-1, simian immunodeficiency virus (siv), and feline immunodeficiency virus (fiv). the mabs prepared by immunization with purified hiv-1 particles were used as antibodies cross-reactive to hiv-1 ... | 1992 | 1326145 |
| sequences within the feline immunodeficiency virus long terminal repeat that regulate gene expression and respond to activation by feline herpesvirus type 1. | we constructed a series of deletion mutants of feline immunodeficiency virus (fiv) long terminal repeat (ltr) to identify the regions that regulate gene expression and are responsive to trans-activation of fiv ltr by feline herpes-virus type 1 (fhv-1). we demonstrated that sequences between -124 and -79, and between -21 and -32 (relative to the cap site) are essential for gene expression of fiv in felis catus whole fetus 4 (fcwf-4) cells. further, we demonstrated that the sequence between -63 an ... | 1992 | 1326814 |
| feline immunodeficiency virus--aids in cats? | 1992 | 1330199 | |
| an early defect in primary and secondary t cell responses in asymptomatic cats during acute feline immunodeficiency virus (fiv) infection. | as in hiv infection of humans, cats infected with fiv are particularly susceptible to secondary infection by opportunistic pathogens, suggesting an impaired ability to elicit an effective immune response against foreign antigens. in order to investigate the development of immunity in fiv-infected cats, we have used an autologous culture system to directly measure priming of naive cd4+ t cells to soluble protein antigen, in vitro. using this assay, we showed previously that cats infected with fiv ... | 1992 | 1458687 |
| [the effectiveness of paramunization for the control of feline coryza]. | 20 cats in a cat home were treated prophylactically and therapeutically with baypamun hk. the animals were allocated into three groups as described. 7 freshly admitted clinically healthy cats were treated prophylactically on day 1, 2 and 9 with 1 ml baypamun hk (group i). 7 cats, who already were allocated for one year in the home and were sick of the feline respiratory disease complex were treated as described for group i (group ii). 6 further cats, who also showed symptoms of the feline respir ... | 1992 | 1524577 |
| cryptococcosis in cats: clinical and mycological assessment of 29 cases and evaluation of treatment using orally administered fluconazole. | twenty-nine cats with naturally occurring cryptococcosis were evaluated prior to commencing oral fluconazole therapy (25-100 mg every 12 h). affected cats ranged from 2 to 15 years-of-age. male cats (19; 66%) and siamese cats (5; 21%) appeared to be over-represented in comparison to the hospital's cat population. mycotic rhinitis was observed in 24 (83%) of the cases, although nasal cavity involvement was subtle in four animals. disease of the skin and subcutaneous tissues was present in 15 case ... | 1992 | 1588463 |
| characterization of morphologic changes and lymphocyte subset distribution in lymph nodes from cats with naturally acquired feline immunodeficiency virus infection. | lymph nodes were collected at biopsy or necropsy from 18 cats with naturally acquired symptomatic feline immunodeficiency virus (fiv) infection and from 18 seronegative cats. thirty-five of the cats were domestic shorthairs and one was a persian cross. the cats ranged from 7 months to 16 years of age and were mainly obtained from california veterinary practitioners, a california cattery, and a veterinary teaching hospital. based on clinical signs present at tissue collection, ten fiv-infected ca ... | 1992 | 1413406 |
| prevalence of feline immunodeficiency virus in submissions of feline serum to a diagnostic laboratory in atlantic canada. | the purpose of this project was to identify the prevalence of feline immunodeficiency virus (fiv) in the atlantic region of canada, and to determine possible associations between fiv serological status and breed, sex, and age. feline serum samples (671) submitted to the prince edward island diagnostic services - atlantic veterinary college laboratory between january 1, 1988 and july 30, 1989 were considered eligible for this study. the majority of samples originated from prince edward island (60 ... | 1992 | 17424114 |
| derivatives of 4-amino-3,6-disulfonato-1,8-naphthalimide inhibit reverse transcriptase and suppress human and feline immunodeficiency virus expression in cultured cells. | we have developed a series of 4-amino-3,6-disulfonato-1,8-naphthalimide (adsn) derivatives in an attempt to create nontoxic compounds effective against lentivirus infections. the adsn derivative lucifer yellow ch ([n-(hydra zinocarbonyl)amino]-4-amino-3,6-disulfonato-1,8-naphthalimid e) (lych) was chosen as a parent compound because of its low toxicity in vivo and in vitro and its tendency to accumulate in monocyte/macrophages, a major reservoir for lentiviruses in vivo. several adsn derivatives ... | 1993 | 7684385 |
| enhanced neutralization of feline immunodeficiency virus by complement viral lysis. | the ability of complement to inactivate feline immunodeficiency virus (fiv) was examined. treatment of virus with complement plus sub-neutralizing titers of antiserum resulted in a significant reduction in the virus titer compared with treatment of the virus with complement or antibody alone. one of the mechanisms by which cat complement inactivates fiv was shown to be by viral lysis as determined by a reverse transcriptase release assay. kinetic studies revealed that viral lysis is initiated so ... | 1993 | 7685130 |
| neutralizing antibodies in cats infected with feline immunodeficiency virus. | sera from cats experimentally infected with five isolates of feline immunodeficiency virus (fiv) from various geographical regions and from fiv enzyme-linked immunosorbent assay-seropositive field cats from four european countries neutralized the petaluma strain of fiv (fiv-p), originally isolated in california, at high titers. in addition, fiv-p and a european isolate proved equally susceptible to neutralization by all sera tested. coupled with observations by fevereiro et al. (m. fevereiro, c. ... | 1993 | 7686186 |
| in vivo lymphocyte tropism of feline immunodeficiency virus. | feline immunodeficiency virus (fiv) infection in the cat is similar to human immunodeficiency virus type 1 infection in causing a selective reduction in cd4+ cell numbers, leading to inversion of the cd4+/cd8+ ratio. to determine whether fiv, similar to human immunodeficiency virus type 1, has a tropism for cd4+ cells, we examined the in vitro and in vivo susceptibilities of feline lymphocyte subpopulations to fiv infection. infection of interleukin-2-dependent cd4+ or cd8+ lymphocyte cultures w ... | 1993 | 7688819 |
| feline immunodeficiency virus infection: plasma, but not peripheral blood mononuclear cell virus titer is influenced by zidovudine and cyclosporine. | the plasma and peripheral blood mononuclear cell (pbmc) titer of feline immunodeficiency virus (fiv) in experimentally infected cats was assessed following administration of either zidovudine or cyclosporine. treatments were begun 24 h post infection (p.i.) and continued for 4 weeks. zidovudine treatment did not prevent establishment of infection with fiv, but plasma virus titers were significantly lower than controls at 2 weeks p.i. this reduction of plasma virus titer by zidovudine was not mai ... | 1993 | 7688949 |
| the feline immunodeficiency virus orf-a gene facilitates efficient viral replication in established t-cell lines and peripheral blood lymphocytes. | frameshift mutants corresponding to all of the identified open reading frames of feline immunodeficiency virus, including the orf-a gene, which has an unknown function, were constructed in vitro. upon transfection into cells, no significant difference between the phenotypes of orf-a mutant clones and those of wild-type clones was demonstrated. although only orf-a mutant virus among all mutant viruses from transfected cells showed infectivity in established t-cell lines, the replication and propa ... | 1993 | 7690413 |
| serological diagnosis of feline immunodeficiency virus infection based on synthetic peptides from env glycoproteins. | feline immunodeficiency virus (fiv) is a lentivirus which infects domestic cats, causing an acquired immunodeficiency syndrome (aids). the aim of the present work was the development of an immunoassay for the diagnosis of fiv infection, using synthetic peptides from fiv envelope (env) glycoproteins. four peptides (8 to 11 amino acids long) corresponding to group-specific epitopes of fiv env extracellular (su) or transmembrane (tm) glycoproteins were synthesized. they were evaluated by enzyme-lin ... | 1993 | 7689247 |
| a monoclonal antibody which blocks infection with feline immunodeficiency virus identifies a possible non-cd4 receptor. | monoclonal antibody vpg15 detects a 24-kda cell surface protein on feline cells permissive for infection with feline immunodeficiency virus (fiv). the antibody blocks infection of fiv-susceptible cells, and expression of the vpg15 marker is decreased in fiv-infected cells in vitro. these results suggest that the antibody may recognize an fiv receptor distinct from cd4. | 1993 | 7679750 |
| detection of b epitopes on the p24 gag protein of feline immunodeficiency virus by monoclonal antibodies. | seven monoclonal antibodies were obtained after immunization of mice with purified sodium dodecyl sulfate (sds)-disrupted feline immunodeficiency virus (fiv). six antibodies specifically bound antigens in the cytoplasm of fiv-infected cells as determined by indirect immunofluorescence and reacted with fiv p24 gag gene product in immunoblots. one reacted positively with virus-infected cells, but failed to recognize fiv structural proteins by immunoblotting. using competition binding studies, the ... | 1993 | 7681301 |
| establishment of a feline t-lymphoblastoid cell line susceptible to feline immunodeficiency viruses. | a feline t-lymphoblastoid cell line susceptible to feline immunodeficiency viruses (fiv) was established and designated as yu-1 cells. yu-1 cells continued to grow over one year with more than 100 successive passages in the presence of human recombinant interleukin-2. surface antigens of yu-1 cells were feline pan-t+, cd4+, and cd8-. susceptibility of yu-1 cells to fiv strains were higher than that of the primary culture of the feline peripheral blood mononuclear cells, indicating that this cell ... | 1993 | 7689139 |
| b epitopes and selection pressures in feline immunodeficiency virus envelope glycoproteins. | in order to map linear b epitopes in feline immunodeficiency virus (fiv) envelope glycoproteins (env), a random library of fiv env polypeptides fused to beta-galactosidase and expressed in escherichia coli was screened by using sera from experimentally fiv-infected cats. we mapped five antibody-binding domains in the surface envelope glycoprotein (su1 to su5) and four in the transmembrane envelope glycoprotein (tm1 to tm4). immunological analysis with 48 serum samples from naturally or experimen ... | 1993 | 7678301 |
| novel, negatively charged, human serum albumins display potent and selective in vitro anti-human immunodeficiency virus type 1 activity. | we prepared a series of modified proteins and peptides by derivatizing the positively charged epsilon-amino groups of the lysine amino acids through reaction with anhydrides of succinic acid (suc) and aconitic acid (aco). human serum albumin (hsa) was modified by introduction of a single carboxylic group (suc-hsa) or two carboxylic groups (aco-hsa) per amine function, yielding strongly negatively charged compounds. the in vitro anti-human immunodeficiency virus (hiv)-1 ic50 of suc-hsa was about ... | 1993 | 7902528 |
| [retrovirus infections of the cat: feline leukemia virus (felv) and feline immunodeficiency virus (fiv). a review from the clinical viewpoint]. | diseases caused by felv are very variable. they can be classified in felv associated, proliferative and neoplastic signs. moreover there are other nonspecific diseases and clinically inapparent infections. felv as well as fiv may cause immunodeficiency syndromes; this is the main problem in fiv infections. in contrast to humans fiv does not induce specific secondary diseases. positive diagnostic results obtained by use of commercial test kits must be verified by other methods. methods of treatme ... | 1993 | 8122242 |
| [feline immunodeficiency virus]. | 1993 | 8105508 | |
| molecular properties of feline immunodeficiency virus (fiv). | 1993 | 8012737 | |
| [demonstration of cell death process by apoptosis in cat lymphocytes infected by fiv (feline immunodeficiency virus)]. | feline immunodeficiency virus (fiv), the causative agent of feline aids, induces a disease syndrome in cats characterized by a decreased lymphocyte-proliferative response to mitogens at all stages of infection and selective depletion of cd4 lymphocyte subsets. in this work, we report that peripheral blood lymphocytes isolated from fiv-infected cats undergo a spontaneous death, in vitro, according to a programmed cell death (pcd) or apoptosis. this phenomenon has also been seen in peripheral bloo ... | 1993 | 8087611 |
| applications of genetic engineering technology in feline medicine. | genetic engineering technology is a rapidly developing field that has almost unlimited potential for the production of safer and more effective vaccines, therapeutic proteins, and more specific and sensitive diagnostic reagents. although applications in veterinary medicine of genetically engineered products are presently limited by availability of species-specific reagents, the use of recombinant dna products is increasing. because of the recent discovery of fiv and the relevance of fiv as an an ... | 1993 | 8093649 |
| feline immunodeficiency virus predisposes cats to acute generalized toxoplasmosis. | this study was designed to examine the effects of a pre-existing, clinically asymptomatic feline immunodeficiency virus (fiv) infection on a primary challenge with toxoplasma gondii. parenteral challenge of fiv-infected cats with tachyzoites of the me49 strain of t. gondii caused a precipitous drop in all lymphocytes (cd4+, cd8+, and b cells) and generalized severe toxoplasmosis. the predominant postmortem lesions included acute and often fatal interstitial pneumonia, dominated histologically by ... | 1993 | 8238262 |
| infection with feline immunodeficiency virus is followed by the rapid expansion of a cd8+ lymphocyte subset. | lymphocyte subset analysis was performed on specific pathogen-free cats infected with feline immunodeficiency virus (fiv). as early as 4 weeks post-infection a sharp rise in lymphocytes expressing the feline cd8 marker (fcd8) occurred. no changes were observed in feline cd4+ (fcd4+) cell number throughout this period. the expanded subset displayed reduced expression of the fcd8 marker (fcd8low) compared to the control population (fcd8high). dual-labelling revealed increased levels of major histo ... | 1993 | 8094707 |
| localization of the viral antigen of feline immunodeficiency virus in the lymph nodes of cats at the early stage of infection. | immunohistochemical examinations of localization of feline immunodeficiency virus (fiv) gag protein were performed on lymph nodes of cats experimentally inoculated with three different strains of fiv (infectious molecular clone of tm 1, petaluma, and kyo-1 strains), using rabbit anti-fiv gag serum. the fiv gag antigens were observed in many follicular dendritic cells (fdcs) and sparsely in small lymphocytes of paracortical area in the lymph nodes of cats inoculated with petaluma and kyo-1 strain ... | 1993 | 8102229 |
| loss of neutrophil and natural killer cell function following feline immunodeficiency virus infection. | experimental infection with the mt. airy isolate of feline immunodeficiency virus (fivma), a lentivirus isolated from a domestic cat exhibiting signs of an immunodeficiency-like syndrome, results in transient lymphadenopathy, fever, stomatitis, enteritis, neurologic abnormalities, and immunosuppression. the effects of fivma infection on neutrophil and natural killer cell (nk) function were examined in vitro. suppression of neutrophil chemiluminescence (cl) responses, as well as reduction in nk-m ... | 1993 | 8105792 |
| delayed hypersensitivity testing as a clinical measure of cell-mediated immunity in the cat. | the purposes of this study were to examine the cell-mediated immune response of the normal cat to the modified live feline viral rhinitis, calicivirus, and parvovirus (fvrcp) vaccine (felocell cvr, norden, lincoln, ne), and to evaluate the intradermal skin test as a clinical measure of the immune response of cats. vaccine and diluent were injected intradermally on the dorsal pinna of 34 normal adult cats. skin thickness measurements, lymphocyte counts, and concanavalin a mitogenesis indices were ... | 1993 | 8256439 |
| the detection and quantification of feline immunodeficiency provirus in peripheral blood mononuclear cells using the polymerase chain reaction. | the polymerase chain reaction method (pcr) was used to detect feline immunodeficiency virus proviral dna in peripheral blood mononuclear cells (pbmc) of a group of 8 experimentally infected cats. the proportion of pbmc containing provirus was determined from 6 to 32 weeks post inoculation (p.i.) by performing pcr on serially diluted samples of pbmc. primers from the p15 and p24 regions of the gag gene were used and southern hybridization using an end-labelled probe was required to confirm primer ... | 1993 | 8128595 |
| effects of preactivated mc540 in the treatment of lymphocytic plasmacytic stomatitis in feline leukemia virus and feline immunodeficiency virus positive cats. | photoactive compounds and drugs are used therapeutically as antibacterial, antiviral and antitumor agents. this report examines the use of a photoactive compound, preactivated merocyanine 540 (pmc540), in the treatment of stomatitis in two cats that are both feline immunodeficiency virus (fiv) positive. one of the cats was also feline leukemia virus (felv) positive. dramatic short term improvement is reported with the dosage regimen and complications. | 1993 | 8148094 |
| evolution of structural proteins of feline immunodeficiency virus: molecular epidemiology and evidence of selection for change. | the dna sequences of structural genes of several u.k. and european isolates of feline immunodeficiency virus (fiv) were determined and compared with those of other worldwide isolates. phylogenetic analyses of both gag and env sequences demonstrate that a japanese isolate represents a distinct sequence subgroup, with corrected amino acid distances to the other isolates averaging 23% in env and 8% in gag. analysis also reveals that an evolutionary radiation of fiv occurred with many isolates diver ... | 1993 | 8383177 |
| detection of feline immunodeficiency virus proviral dna in feline peripheral blood mononuclear cells by the nested two-step polymerase chain reaction. | the polymerase chain reaction (pcr) was applied to detect feline immunodeficiency virus (fiv) proviral dnas in primary peripheral blood mononuclear cells (pbmc). suitable conditions for pcr amplification were examined to obtain highly sensitive and specific results by simple staining in agarose gel. specific amplification of fiv proviral dna in pbmc dna of fiv-infected cats was achieved by a nested two-step pcr that amplified the dna first with outer primers and then with inner primers nested wi ... | 1993 | 8219498 |
| clinical and laboratory findings in chronic conjunctivitis in cats: 91 cases (1983-1991). | clinical findings and laboratory test results from 91 cats with chronic conjunctivitis were studied to determine the causes of the disease and the sensitivity of diagnostic procedures used, and to identify correlations between results of various diagnostic procedures and clinical or signalment variations. mean age of affected cats was 2.9 +/- 2.7 years (+/- sd), with a range from 1 month to 11 years. conjunctivitis was more likely to be bilateral (56 cats) than unilateral (35 cats). in cats test ... | 1993 | 8226238 |
| polymerase chain reaction and partial sequencing of a british isolate (t637) of feline immunodeficiency virus. | we have used the polymerase chain reaction (pcr) and direct sequencing of the amplified products to obtain information of the molecular nature of an fiv isolate, t637. cats experimentally infected with t637 have progressed to clinical immunodeficiency disease. the 5' long terminal repeat (ltr), most of the genes coding for internal proteins (gag) and surface proteins (env), and part of the polymerase (pol) gene have been sequenced. the ltr of t637 has 92% nucleic acid identity with the prototype ... | 1993 | 8273281 |
| feline immunodeficiency virus infection of cats as a model to test the effect of certain in vitro selection pressures on the infectivity and virulence of resultant lentivirus variants. | three groups of specific pathogen-free (spf) domestic cats, each containing 5 animals, were infected with one of three closely related fiv variants and monitored for 36 weeks. a fourth group of 5 cats was sham-infected and served as uninfected controls. fiv variants included: (1) a fully virulent animal passaged fiv-petaluma; (2) a crandell feline kidney (crfk) cell-adapted fiv-petaluma (fiv-crfk); and (3) a variant of fiv-crfk (fiv-crfkazt) that had been selected in vitro for resistance to azid ... | 1993 | 8279815 |
| the ap-1 binding site in the feline immunodeficiency virus long terminal repeat is not required for virus replication in feline t lymphocytes. | sequences of 31 bp containing putative ap-1 and ap-4 binding sequences in the u3 region of the feline immunodeficiency virus (fiv) long terminal repeat (ltr) were deleted and the basal promoter activity of the ltr was measured by the chloramphenicol acetyltransferase (cat) assay. the activity of the fiv ltr was reduced in felis catus whole foetus 4 (fcwf-4) cells and crandell feline kidney cells by this deletion. cotransfection of murine c-fos or c-jun expression plasmids with the fiv ltr-cat re ... | 1993 | 8393913 |
| renal involvement in feline immunodeficiency virus infection: a clinicopathological study. | renal tissues from 15 cats naturally infected with feline immunodeficiency virus (fiv) were examined histologically, immunohistochemically and ultrastructurally. renal function and urinary proteins were also studied. kidney abnormalities were found in 12 cats and were characterized by mesangial widening with segmental to diffuse glomerulosclerosis and presence of igm and c3, and scanty igg deposits in the mesangium. tubulointerstitial lesions were also present. in 6 cats the lesions were severe ... | 1993 | 8321363 |
| sequence and functional characterization of feline interleukin 2. | since the discovery of its involvement in the pathogenesis of feline immunodeficiency virus infection ("cat aids") and feline leukemia virus infection, the role of feline interleukin 2 (il-2) has been a focus of particular interest. the purpose of this study was to clone feline il-2 cdna, as well as synthesize bioactive recombinant feline il-2. the isolation of cdna encoding feline il-2 was carried out using a pcr-based strategy and screening of a feline leukocyte cdna library. feline il-2 consi ... | 1993 | 8352761 |
| experimental vaccine protection against homologous and heterologous strains of feline immunodeficiency virus. | more than 90% of cats immunized with inactivated whole infected-cell or cell-free feline immunodeficiency virus (fiv) vaccines were protected against intraperitoneal infection with 10 50% animal infectious doses of either homologous fiv petaluma (28 of 30 cats) or heterologous fiv dixon strain (27 of 28 cats). all 15 control cats were readily infected with either strain of fiv. protection appears to correlate with antiviral envelope antibody levels by a mechanism yet to be determined. | 1993 | 8380095 |
| structure and variations of feline immunodeficiency virus envelope glycoproteins. | we report the characterization of the env gene of a feline immunodeficiency virus isolate from france (fiv wo). fiv wo gag and env genes were cloned directly from cat peripheral blood mononuclear cells, using polymerase chain reaction. the env molecular clone was shown to be functional and to express antigenically relevant envelope glycoproteins in vitro. alignment of fiv wo sequences with available fiv sequences and application of a regionalization algorithm resulted in delineation of variable ... | 1993 | 8380668 |
| prophylactic zdv therapy prevents early viremia and lymphocyte decline but not primary infection in feline immunodeficiency virus-inoculated cats. | prophylactic zidovudine (zdv) therapy was evaluated in the feline immunodeficiency virus (fiv)-inoculated cat model for hiv-1 infection in humans. zdv treatment (30 mg/kg/day via continuous subcutaneous infusion) was initiated 48 h prior to virus inoculation and continued for 28 days. transient plasma antigenemia evident in six of six untreated cats at week 2 post-inoculation (pi) was absent in the zdv-treated cats although at 10 and 14 weeks pi (6 and 10 weeks after drug treatment), one of the ... | 1993 | 8381867 |
| post-translational processing of the feline immunodeficiency virus envelope precursor protein. | the synthesis and processing of the envelope glycoprotein precursor of the feline immunodeficiency virus (fiv) isolate fiv-ut113 was investigated in a persistently infected crandell feline kidney cell line (crfk) and in an eukaryotic expression system. pulse-chase studies showed two glycoproteins after a 5 min pulse-labeling: a gp150 and a gp130 species. during a 30-min chase the gp150 species disappeared almost completely while gp130 increased proportionally; it was subsequently processed into ... | 1993 | 8382405 |
| biological nature of feline immunodeficiency virus. | feline immunodeficiency virus (fiv) was first isolated in 1986 from a cat with an acquired immunodeficiency syndrome (aids)-like disease. this virus has many characteristics in common with human immunodeficiency virus which is an etiological agent of aids in human and is classified as a member of the lentivirus genus of the retrovirus family. since the discovery of fiv, many researchers have studied the virus extensively from clinical, biological, and genetic aspects. in this review, the biologi ... | 1993 | 8399727 |
| expression of feline immunodeficiency virus gag and env precursor proteins in spodoptera frugiperda cells and their use in immunodiagnosis. | the gag and env genes of the feline immunodeficiency virus strain ut113 were cloned into a baculovirus transfer vector. the recombinant plasmids were used to create recombinant baculoviruses that expressed either the gag or the env precursor protein in insect cells (sf9 cells). leader sequence cleavage occurred in sf9 cells expressing the envelope precursor, but further processing was not observed. crude lysates of insect cells infected with the wild-type baculovirus or with the recombinant viru ... | 1993 | 8408554 |
| seroprevalence of infectious disease agents in free-ranging florida panthers (felis concolor coryi). | serum samples obtained from 38 free-ranging florida panthers (felis concolor coryi) in southern florida, march 1978 through february 1991, were tested for antibodies against eight bacterial, parasitic, and viral disease agents. sera were positive for antibodies against feline panleukopenia virus (fpv) (78%), feline calicivirus (56%), feline immunodeficiency virus/puma lentivirus (37%), feline enteric coronavirus/feline infectious peritonitis virus (19%), and toxoplasma gondii (9%). all samples w ... | 1993 | 8445789 |
| identification of proteolytic processing sites within the gag and pol polyproteins of feline immunodeficiency virus. | n-terminal amino acid sequencing, ion spray mass spectrometry, and cleavage of synthetic peptide substrates were used to identify the n and c termini of the mature gag and pol proteins of feline immunodeficiency virus (fiv). the gag polyprotein encodes matrix (ma), capsid (ca), and nucleocapsid (nc) proteins. the gag-pol polyprotein encodes, in addition to the above proteins, protease (pr), reverse transcriptase (rt), dutpase (du), and integrase (in). secondary cleavage of rt at trp-595-tyr-596 ... | 1993 | 8383214 |
| a single amino acid substitution in hypervariable region 5 of the envelope protein of feline immunodeficiency virus allows escape from virus neutralization. | we infected a specific-pathogen-free cat (cat 14) with molecularly cloned feline immunodeficiency virus clone 19k1 (fiv19k1 [k. h. j. siebelink, i. chu, g. f. rimmelzwaan, k. weijer, a. d. m. e. osterhaus, and m. l. bosch, j. virol. 66:1091-1097, 1992]). serum of this cat obtained 22 weeks postinfection (serum 1422) neutralized fiv19k1 but not fiv19k32, which is 99.3% identical to fiv19k1 in the envelope gene. serum 1422 also neutralized virus isolated from cat 14 at weeks 2 and 32 postinfection ... | 1993 | 8383237 |
| passive antibody protection of cats against feline immunodeficiency virus infection. | all six cats passively immunized with sera from either feline immunodeficiency virus (fiv)-vaccinated cats or cats infected with fiv (petaluma strain) were protected from homologous fiv infection at a challenge dose that infected all six control cats. passive immunization with sera from cats vaccinated with uninfected allogeneic t cells used to grow the vaccine virus did not protect either of two cats against the same fiv challenge. these results suggest that antiviral humoral immunity, perhaps ... | 1993 | 8383246 |
| detection of feline immunodeficiency virus in saliva and plasma by cultivation and polymerase chain reaction. | the rates of feline immunodeficiency virus (fiv) isolation from saliva, plasma, and peripheral blood mononuclear cells (pbmc) of infected cats were compared; isolation rates were 18, 14, and 81%, respectively, in naturally infected cats and 25, 57, and 100%, respectively, in experimentally infected animals. there was no obvious relationship between isolation rate and clinical stage or between isolation rate and the titer of neutralizing antibody in serum. virus could be isolated from one salivar ... | 1993 | 8384624 |
| apoptosis induced by tumor necrosis factor in cells chronically infected with feline immunodeficiency virus. | tumor necrosis factor alpha (tnf-alpha) induced morphologic changes such as chromatin condensation and cell shrinkage in a feline fibroblastic cell line (crfk) chronically infected with feline immunodeficiency virus (fiv) but not in uninfected crfk cells. dna extracted from tnf-alpha-treated crfk cells infected with fiv showed a ladder of nucleosomal dna, indicating that this cytocidal effect by tnf-alpha was due to programmed cell death, or apoptosis. these findings may have implications for un ... | 1993 | 8386260 |
| retrovirus infections of the nervous system. | mammalian retroviruses of all three subfamilies infect the nervous system. the leukemia viruses (oncovirinae) and lentiviruses (lentivirinae, eg, human immunodeficiency virus) cause serious disease, while the foamy viruses (spumavirinae) have not yet been shown to cause any disease. this review illustrates these diseases by referring particularly to three viruses: the human and murine leukemia viruses (human t-cell leukemia-lymphoma virus type i and murine leukemia virus), and the human immunode ... | 1993 | 8386955 |
| feline cd4 molecules expressed on feline non-lymphoid cell lines are not enough for productive infection of highly lymphotropic feline immunodeficiency virus isolates. | to investigate whether the feline cd 4 (fcd4) molecules are involved in infections of highly lymphotropic feline immunodeficiency virus (fiv) isolates, we expressed fcd4 stably on crandell feline kidney cells and felis catus whole foetus 4 cells by transfection of a cdna encoding the fcd4 glycoprotein, and then infected them with tm 1 and tm 2 strains of fiv, which are unable to infect these cells productively. in spite of fcd 4 being expressed on these cells, no virus production was observed. t ... | 1993 | 8389115 |
| evaluation of cofactor effect of feline syncytium-forming virus on feline immunodeficiency virus infection. | although feline immunodeficiency virus (fiv) and the unrelated retrovirus feline leukemia virus (felv) are associated with acquired immune deficiency in cats, experimental and field evidence indicates that coinfection with both viruses may lead to more serious disease syndrome. a third feline retrovirus, feline syncytium-forming virus (fesfv), which is far more prevalent than either fiv or felv and is considered nonpathogenic in nature, is consistently coisolated from sick, fiv-infected cats. to ... | 1993 | 8391229 |
| aids-associated encephalopathy with experimental feline immunodeficiency virus infection. | experimental intravenous challenge of 8-week-old kittens with the feline immunodeficiency virus maryland isolate (fiv-md) was investigated for its ability to infect the central nervous system (cns) and induce neurologic abnormalities. six cats were inoculated with 1,000 tcid50 units of fiv-md isolate, with six age-matched cats serving as uninfected controls. clinical and immunological evaluation documented that challenged cats developed immunodeficiency and growth delay. neurologic examination r ... | 1993 | 8389849 |
| feline immunodeficiency virus infection. clinicopathologic findings in 90 naturally occurring cases. | in 90 cats with naturally occurring feline immunodeficiency virus (fiv) infection, the clinicopathologic changes seen at the time of first diagnosis of fiv infection included lymphopenia (29%), neutrophilia (27%), monocytosis (23%), anemia (18%), leukocytosis (13%), leukopenia (13%), neutropenia (11%), hyperproteinemia (38%), and hyperglobulinemia (25%). forty-nine (54%) of the cats showed multiple hematologic abnormalities, and a further 24 (17%) had a single abnormality. the most consistent ch ... | 1993 | 8388953 |
| current thoughts on feline immunodeficiency virus infection. | the feline immunodeficiency virus (fiv) is a novel feline retrovirus of the lentivirus subfamily that is known to induce immunodeficiency disorders in experimentally and naturally infected cats. recent studies have characterized the stages of infection and specific immune deficits associated with fiv infection. host cell populations harboring the virus have been better elucidated. molecular cloning and nucleotide sequence analysis of the genomes of several isolates of fiv indicate sequence varia ... | 1993 | 8380656 |
| the diagnosis and epidemiology of feline immunodeficiency virus (fiv) infection in the netherlands. | 1993 | 8386863 | |
| chronic gingivitis in a colony of cats infected with feline immunodeficiency virus and feline calicivirus. | the inadvertent introduction of feline calicivirus into a colony of 19 experimental cats, 13 of which were infected with feline immunodeficiency virus, resulted in the development of chronic gingivitis in nine animals. of these, six were infected with both viruses, one with the immunodeficiency virus alone and two with the calicivirus alone. the gingivitis was generally more severe in the cats infected with both viruses, suggesting that feline immunodeficiency virus may make cats infected with c ... | 1993 | 8387710 |
| programmed cell death (apoptosis) as a mechanism of cell death in peripheral blood mononuclear cells from cats infected with feline immunodeficiency virus (fiv). | fiv is a lentivirus infection of cats which induces an immunodeficiency syndrome associated with early qualitative defects in antigen-specific t cell function and with late quantitative defects in cd4+ t lymphocytes. we have observed that peripheral blood mononuclear cells (pbmc) from fiv-infected cats have impaired survival in culture. the mechanism of this in vitro dysfunction and depletion is not known. we have proposed that inappropriate induction of programmed cell death (apoptosis) could a ... | 1993 | 8391941 |
| distinct superinfection interference properties yet similar receptor utilization by cytopathic and noncytopathic feline leukemia viruses. | cell killing by cytopathic retroviruses is often associated with a delay or failure in the establishment of superinfection interference. superinfection has been observed during t-cell killing and fatal immunodeficiency disease induction by the feline leukemia virus (felv) chimera felv-faids-eecc, containing the surface envelope glycoprotein (su) of felv-faids clone 61c. we demonstrate here that 61c su has a defect that results in a nearly complete failure to establish superinfection interference ... | 1993 | 8394443 |
| molecular cloning and characterization of deoxyuridine triphosphatase from feline immunodeficiency virus (fiv). | the feline lentivirus, fiv, contains dutpase (du) as part of the enzyme cassette encoded by the viral pol gene (elder et al., 1992, j. virol. 66, 1791-1794). the enzyme is processed from the pol polyprotein and is packaged into infectious virions. we report here the basic characteristics of the viral enzyme, including substrate specificity, ion requirements, and ph optimum. we also report the overexpression of du in escherichia coli and insertional mutagenesis of the enzyme in the context of the ... | 1993 | 8396797 |
| nucleotide sequences of australian isolates of the feline immunodeficiency virus: comparison with other feline lentiviruses. | proviral dna from four australian isolates of feline immunodeficiency virus (fiv) was amplified by pcr and the nucleotide sequence determined for two conserved regions within gag (p15/p24) and pol (rt) genes. comparison with the nucleotide and deduced amino acid sequence of two previously described u.s. isolates from california (petaluma and ppr), and a third from maryland (md) as well as the japanese isolate tm2, revealed a close similarity between the australian and californian isolates with 9 ... | 1993 | 8397502 |
| identification of a region in the pr55gag-polyprotein essential for hiv-1 particle formation. | the pr55gag polyprotein of hiv-1 plays a critical role in the formation of immature virus particles in the cell and during the budding process. we investigated the influence of amino acid substitutions in the p24ca- region of the gag polyprotein on the viral assembly process. deletion of the amino acids 341-352 in the carboxy terminal part of the p24ca resulted in a loss of the capacity of the gag polyprotein to form virus-like particles when expressed in eucaryotic cells by recombinant vaccinia ... | 1993 | 8460500 |
| molecular cloning and characterization of a cdna encoding feline interleukin-6. | with the discovery that the cat is susceptible to infection by a lentivirus quite similar to the virus that causes the human acquired immune deficiency syndrome, the cat has become an important animal model with which the interactions between host and immunodeficiency virus can be studied. to facilitate investigation of the feline immune response and the response of cytokine expression to infection by feline immunodeficiency virus, our laboratory has focused on the isolation and molecular clonin ... | 1993 | 8234373 |
| use of a feline panleukopenia modified live virus vaccine in cats in the primary-stage of feline immunodeficiency virus infection. | cats in the primary stage of feline immunodeficiency virus (fiv) infection and fiv seronegative cats were vaccinated with a modified live feline panleukopenia virus vaccine (fpv-mlv). the fpv-mlv strain was not pathogenic for fiv seronegative cats, whereas in fiv infected cats it produced severe clinical signs, similar to those typically observed in cats naturally infected with field strains of fpv (fever, diarrhoea, leukopenia). | 1993 | 8237206 |
| small animal model of aids and the feline immunodeficiency virus. | 1993 | 8237595 | |
| extensive sequence variation of feline immunodeficiency virus env genes in isolates from naturally infected cats. | in an investigation of the evolution of feline immunodeficiency virus (fiv) in vivo, sequential isolates from a persistently infected cat were examined by direct sequencing following amplification of selected subgenomic regions by polymerase chain reaction (pcr). three isolates, t90, t91, and t92, obtained over a three-year period revealed no changes to regions known to be conserved within gag and pol genes. additionally, no change occurred within gag and pol in an isolate recovered from a secon ... | 1993 | 8240017 |
| effect of feline immunodeficiency virus infection on toxoplasma gondii-specific humoral and cell-mediated immune responses of cats with serologic evidence of toxoplasmosis. | serum samples from 89 cats with serologic evidence of toxoplasmosis were identified by using an enzyme-linked immunosorbent assay (elisa) that detected toxoplasma gondii-specific immunoglobulin m (igm) or t. gondii-specific immunoglobulin g (igg). concurrent feline immunodeficiency virus (fiv) infection was detected in 36 cats using an elisa for detection of fiv-specific igg. the majority of the cats in both the fiv-seropositive and fiv-seronegative groups were male and > 5 years of age. fiv-ser ... | 1993 | 8388954 |
| fusion activity dissociated from replication ability in feline immunodeficiency virus (fiv) in human cells. | multinucleated-giant-cell formation followed by cell killing was observed after cocultivation of the feline immunodeficiency virus (fiv)-producing feline t-cell line 3201/fiv with various human cells, including t-cell lines carrying human t-cell lymphotropic virus type i (htlv-i). the susceptibility to giant cell formation varied with the cell lines tested. cocultivation of irradiated 3201/fiv cells with mt-2 cells resulted in giant cell formation as early as 2 h in culture, with striking resemb ... | 1993 | 8254466 |