Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of the 5' and 3' ends of viral messenger rnas isolated from bhk21 cells infected with germiston virus (bunyavirus). | the 3' ends of the s and m messenger rnas isolated from bhk21 cells infected with germiston virus were analyzed by mapping with rnase t2 or nuclease s1. the transcription termination signal was found to be located approximately 115 and 80 nucleotides upstream from the 3' end of the s and m genomic rna templates, respectively. both mrnas were found to possess several adenosine residues at their 3' ends, but were not polyadenylated. they have acquired at their 5' end a heterologous 12- to 18-nucle ... | 1990 | 2309451 |
| coding strategy of the s rna segment of dugbe virus (nairovirus; bunyaviridae). | the s rna segment of dugbe (dug) virus (nairovirus; bunyaviridae) was sequenced from three overlapping cdna clones and by primer extension. the s rna is 1712 nucleotides in length and contains one large open reading frame (orf) of 1326 nucleotides coding for a 49.4-kda protein on viral complementary (vc) rna. this protein in size corresponds to the dug nucleocapsid (n) protein (p. cash, 1985, j. gen. virol. 66, 141-148). the 49.4-kda product was expressed as a fusion protein with beta-galactosid ... | 1990 | 2327076 |
| heterologous reassortment of bunyaviruses in aedes triseriatus mosquitoes and transovarial and oral transmission of newly evolved genotypes. | aedes triseriatus mosquitoes were orally infected with two different california serogroup bunyaviruses (la crosse and snowshoe hare viruses) and high frequency reassortment occurred in these mosquitoes. increased viral replication and subsequent gene segment reassortment was noted in the ovaries of mosquitoes that had ingested multiple blood-meals. to determine whether newly generated reassortant viruses could be transmitted transovarially to progeny mosquitoes, adult female mosquitoes were inoc ... | 1990 | 2345365 |
| quantitative in situ hybridization using strand specific rna probes: expression of the bunyavirus germiston s segment in mosquito cells. | infection of vero (monkey) cells by germiston bunyavirus is highly cytopathic with cell lysis and virus production at a high titre, whereas infection of aedes albopictus c6/36 (mosquito) cells leads, after an acute primary phase, to a persistent non-cytopathic infection with a loss in virus production. in this report we demonstrate that single-stranded rna probes can be successfully used in an in situ hybridization assay to quantify viral expression during this persistent infection. the steady-s ... | 1990 | 2402248 |
| isolation and characterization of sedlec virus, a new bunyavirus from birds. | a pathogenic agent designated av 172 was isolated from the blood of a reed warbler (acrocephalus scirpaceus) out of 767 samples from birds belonging to 35 species and 14 families. the birds (largely wetland passerines) were captured in the reed-belt littoral of nesyt fishpond in southern moravia, czechoslovakia, during the years 1984 to 1987. virus av 172 has been found to represent probably a new species (designated virus "sedlec") of family bunyaviridae. sedlec virus is pathogenic to suckling ... | 1990 | 1981444 |
| the s rna segment of tomato spotted wilt virus has an ambisense character. | the complete nucleotide sequence of the s rna of tomato spotted wilt virus (tswv) was determined. the rna is 2916 nucleotides long and has an ambisense coding strategy. the sequence contains two open reading frames (orfs), one in the viral sense which encodes a protein with a predicted mr of 52.4k and one in the viral complementary sense which encodes the viral nucleocapsid protein of mr 28.8k. both proteins are expressed by translation of two subgenomic rna species that possibly terminate at a ... | 1990 | 1693160 |
| comparative therapeutic efficacy of recombinant interferons-alpha, -beta, and -gamma against alphatogavirus, bunyavirus, flavivirus, and herpesvirus infections. | recombinant (r) preparations of interferons (ifn)-alpha, -beta, and -gamma were shown to protect mice against experimental virus infections with herpes simplex virus type 2 (hsv-2), and with three rna-containing viruses from different families: banzi, a flavivirus; semliki forest virus (sfv), an alphatogavirus; and caraparu, a bunyavirus. the antiviral effects of the three different types of ifn were different with each virus. hsv-2 was the most sensitive virus, followed by sfv. against banzi vi ... | 1990 | 1696607 |
| anti-mrnas in la crosse bunyavirus-infected cells. | unlike some members of the family bunyaviridae which contain ambisense genomes, all la crosse virus reading frames are translated from antigenome sense mrnas. nevertheless, la crosse virus genome sense mrnas or anti-mrnas are initiated from antigenome templates. these are characterized by the same range of capped, nontemplated sequences at their 5' ends as mrnas, but their 3' ends are presumed to be heterogenous, as they were not seen on rna blots. the anti-mrnas are estimated to be 15 to 30 tim ... | 1990 | 1697911 |
| antigenic variance. | 1991 | 1718665 | |
| [interactions between mycoplasma and human and animal viruses. ultrastructural analysis]. | presented are the data on the ultrastructural analysis of interaction between mycoplasma and certain cancerogenic and infectious viruses in humans and animals. revealed are spontaneous associations of mycoplasma with viruses of cattle leukemia, t-cell human leukemia and with a representative of bunyaviruses. immediate interaction of these agents is found possible. simulated complexes of mycoplasma with infectious viruses are developed. electron microscopy on supramolecular levels revealed immedi ... | 1991 | 1656621 |
| [the circulation of viruses of the california encephalitis and bunyamwera groups (bunyaviridae, bunyavirus) on the northeastern russian plain]. | in the komi assr, 102.2 thousand mosquitoes, 207 small mammals, 1487 blood serum specimens from people, 793 cow blood sera, 140 blood serum specimens from reindeer were collected in june-august, 1988. twenty seven virus strains isolated from mosquitoes were classified into bunyaviridae family according to the data of electron microscopic studies. identification of the isolates by cft and ifa showed 18 of the strains to belong to california encephalitis complex and 9 to bunyamwera complex. serolo ... | 1991 | 1677507 |
| isolation of northway serotype and other bunyamwera serogroup bunyaviruses from california and oregon mosquitoes, 1969-1985. | eight previously untyped bunyamwera serogroup bunyaviruses that had been isolated from mosquitoes collected in california and oregon between 1969 and 1985, were identified by cross-neutralization tests. four viruses from anopheles freeborni and a virus from aedes sierrensis collected in butte county in the central valley of california in 1970-71 were shown to belong to the northway serotype. the existence of a northway serotype virus in california had been inferred from previous serologic survey ... | 1991 | 1677543 |
| bunyavirus rna synthesis: genome transcription and replication. | 1991 | 1682111 | |
| arthropod vectors in the evolution of bunyaviruses. | viruses from each genus of bunyaviridae have preferential relationships to the arthropods of only one or two families, i.e. bunyavirus to mosquitoes (culicidae), phlebovirus to sand flies (psychodidae) and mosquitoes, uukuvirus and nairovirus to ticks (ixodidae and argasidae). an exception is genus hantavirus not proven to be transmitted by vectors. within the bunyavirus genus 16 serogroups have been recognized on the basis of their antigenic relationship. based on isolations from the nature eac ... | 1991 | 1683124 |
| vertical and venereal transmission of california group viruses by aedes triseriatus and culiseta inornata mosquitoes. | aedes triseriatus and culiseta inornata mosquitoes were compared in their ability to transmit vertically la crosse (lac) and snowshoe hare (ssh) viruses. lac virus was transovarially transmitted by 53% of ae. triseriatus, the natural vector, and by 22% of cs. inornata mosquitoes. ssh virus was transovarially transmitted by 89% of cs. inornata, a proposed natural vector, and by 29% of ae. triseriatus. a genetic approach, using lac, ssh, and lac/ssh reassortant viruses was then used to elucidate v ... | 1991 | 1686962 |
| assembly and polarized release of punta toro virus and effects of brefeldin a. | punta toro virus (ptv), a member of the sandfly fever group of bunyaviruses, is assembled by budding at intracellular membranes of the golgi complex. we have examined ptv glycoprotein transport, assembly, and release and the effects of brefeldin a (bfa) on these processes. both the g1 and g2 proteins were transported out of the endoplasmic reticulum (er) and retained in the golgi complex in a stable structure, either during ptv infection or when expressed from a vaccinia virus recombinant. bfa t ... | 1991 | 1847462 |
| neuroattenuation of an avirulent bunyavirus variant maps to the l rna segment. | the derivation and characterization of a neuroattenuated reassortant clone (rfc 25/b.5) of california serogroup bunyavirus was described previously (m. j. endres, a. valsamakis, f. gonzalez-scarano, and n. nathanson, j. virol. 64:1927-1933, 1990). to map the rna segment responsible for this attenuation, a panel of reassortants was constructed between the attenuated clone b.5 (genotype tll) and a virulent clone (b1-1a) of reciprocal genotype (ltt). parent viruses and clones representing all of th ... | 1991 | 1895395 |
| golgi complex localization of the punta toro virus g2 protein requires its association with the g1 protein. | the glycoproteins of bunyaviruses accumulate in membranes of the golgi complex, where virus maturation occurs by budding. in this study we have constructed a series of full length or truncated mutants of the g2 glycoprotein of punta toro virus (ptv), a member of the phlebovirus genus of the bunyaviridae, and investigated their transport properties. the results indicate that the hydrophobic domain preceding the g2 glycoprotein can function as a translocational signal peptide, and that the hydroph ... | 1991 | 1905078 |
| california serogroup virus infections in the ryazan region of the ussr. | serum samples from 60 people diagnosed in the summer of 1989 as having acute respiratory infections, meningitis or meningoencephalitis, acute pneumonia, lyme disease, or other illnesses were tested by neutralization and hemagglutination-inhibition with tahyna, inkoo, and snowshoe hare viruses of the california serogroup (family bunyaviridae, genus bunyavirus). demonstration of a diagnostic increase in antibody titers between paired serum samples from seven people showed that their illnesses were ... | 1991 | 1928573 |
| a serologic study of california serogroup bunyaviruses in sri lanka. | a seroepidemiologic study of california (cal) serogroup viruses (genus bunyavirus, family bunyaviridae) was carried out on 952 human and 1,834 animal sera collected from different ecological zones of sri lanka (latitudes 5-10 degrees n, longitudes 79-82 degrees e). the sera were screened for neutralizing antibody to lumbo (lum), snowshoe hare (ssh), and trivittatus (tvt) viruses by plaque neutralization tests on vero cells. of 2,786 sera screened, 262 (9.5%) had antibody to one or more viruses t ... | 1991 | 1928574 |
| bunyavirus protein transport and assembly. | 1991 | 1935227 | |
| bunyaviridae--natural history. | obviously, the family bunyaviridae is comprised of a large number of epidemiologically diverse viruses. they vary dramatically in their vector and vertebrate host relationships, geographic distributions, and epidemic potential in humans and animals. public health practitioners, veterinarians, virologists, entomologists, biologists, ecologists, molecular biologists, and other scientists will all benefit from increased study and knowledge of this fascinating group of viruses. | 1991 | 1935229 |
| transmission of a newly recognized virus (bunyaviridae, bunyavirus) isolated from aedes albopictus (diptera: culicidae) in potosi, missouri. | aedes albopictus (skuse) mosquitoes collected in potosi, mo., were tested for their ability to transmit a newly recognized bunyamwera sero group virus isolated from the same mosquito population. mosquitoes were fed artificial blood meals containing 4.5-6.2 log10 tcid50 of virus per ml. after 7-29 d at 25 degrees c, 79-99% of the mosquitoes had disseminated infections and 0-26% transmitted virus to fluid-filled capillary tubes. transmission was first observed after 7 d of extrinsic incubation. te ... | 1991 | 1941926 |
| reassortment of la crosse and tahyna bunyaviruses in aedes triseriatus mosquitoes. | experiments were conducted to determine if la crosse (lac) and tahyna (tah) viruses reassort in aedes triseriatus mosquitoes and to determine the genotypic frequencies of viruses selected by in vivo vector interactions. a molecular hybridization technique was used to analyze progeny viruses. probes specific for the la crosse l, m and s segments (plac4.16: lac l rna; plac4.27: lac m rna; plac4c-26: lac s rna) were used to determine the parental origin of the progeny rna segments. following infect ... | 1991 | 1950171 |
| formation and intracellular transport of a heterodimeric viral spike protein complex. | we have analyzed the heterodimerization and intracellular transport from the er to the golgi complex (gc) of two membrane glycoproteins of a bunyavirus (uukuniemi virus) that matures by a budding process in the gc. the glycoproteins g1 and g2, which form the viral spikes, are cotranslationally cleaved in the er from a 110,000-d precursor. newly synthesized g1 was transported to the gc and incorporated into virus particles about 30-45 min faster than newly synthesized g2. analysis of the kinetics ... | 1991 | 1988460 |
| germiston virus transcriptase requires active 40s ribosomal subunits and utilizes capped cellular rnas. | the transcriptase associated with germiston virus was assayed in an in vitro reaction in which transcription was coupled to translation by adding reticulocyte lysate under the appropriate salt conditions. when analyzed in polyacrylamide gels, the major transcripts migrated like authentic s mrnas and possessed 12- to 18-base-long nontemplated 5' extensions similar to the 5' end of viral mrnas. these transcripts were functional for the synthesis of at least proteins n and nss. when translation was ... | 1992 | 1731108 |
| tacaribe arenavirus rna synthesis in vitro is primer dependent and suggests an unusual model for the initiation of genome replication. | a tacaribe virus in vitro system for rna synthesis was established and found in large part to faithfully reproduce rna synthesis in vivo. similar to influenza virus and bunyavirus in vitro systems, this system was also highly dependent on added oligonucleotides. of the eight tested, only three were active, in the order gpc greater than cpg greater than apapc. determination of the 5' ends of the transcripts suggested that the oligonucleotides were acting as primers. in particular, whereas stimula ... | 1992 | 1738196 |
| the effect of cicloxolone sodium on the replication in cultured cells of adenovirus type 5, reovirus type 3, poliovirus type 1, two bunyaviruses and semliki forest virus. | the effect of cicloxolone sodium (ccx) on the replication of typical representatives of different virus families [adenovirus type 5 (ad-5), reovirus type 3 (reo-3), bunyamwera and germiston viruses, poliovirus type 1 (polio-1) and semliki forest virus (sfv)] in tissue culture was investigated. the golgi apparatus inhibitor monensin (mon) and ccx were shown to have analogous effects on some aspects of virus replication. although the mon-like effect of ccx played no role in the antiviral activity ... | 1992 | 1311362 |
| cell biology of viruses that assemble along the biosynthetic pathway. | in this review we discuss five groups of viruses that bud into, or assemble from, different compartments along the biosynthetic pathway. these are herpes-, rota-, corona-, bunya- and pox-viruses. our main emphasis will be on the virally-encoded membrane glycoproteins that are responsible for determining the site of virus assembly. in a number of cases these proteins have been well characterized and appear to serve as resident markers of the budding compartments. the assembly and dissemination of ... | 1992 | 1333835 |
| further characterization of a broad-spectrum antiviral substance in human serum. | a broadly active antiviral glycoprotein (uti beta) occurs naturally in human sera at an average antiviral titer of 50 u/ml. this inhibitor is active against all virus families tested to date, including representative poxviruses, herpesviruses, enteroviruses, paramyxoviruses, alpha-viruses, flaviviruses, bunyaviruses, and rhabdoviruses. it is a glycoprotein of approximately 60,000 +/- 10,000 da, which is stable at ph 2 to 10 and at 80 degrees c for up to 10 min. mild oxidation with naio4 and trea ... | 1992 | 1335259 |
| antiviral (rna) activity of selected amaryllidaceae isoquinoline constituents and synthesis of related substances. | a series of 23 amaryllidaceae isoquinoline alkaloids and related synthetic analogues were isolated or synthesized and subsequently evaluated in cell culture against the rna-containing flaviviruses (japanese encephalitis, yellow fever, and dengue viruses), bunyaviruses (punta toro, sandfly fever, and rift valley fever viruses), alphavirus (venezuelan equine encephalomyelitis virus), lentivirus (human immunodeficiency virus-type 1) and the dna-containing vaccinia virus. narciclasine [1], lycoricid ... | 1992 | 1336040 |
| [characterization and antigenic relationship of 3 new bunyaviruses in the group anopheles a (bunyaviridae) of arboviruses]. | the isolation and characterization of three new viruses obtained from the tucuruí hydroelectric dam region is reported. these three agents belong to the anopheles a serogroup, genus bunyavirus, bunyaviridae. the tucuruí (tuc), caraipé (cpe) and arumateua (art) viruses have close relationships with each other and with trombetas (tbt) virus, an anopheles a virus previously isolated in the amazon region of brazil. these viruses form the "trombetas complex". tuc, cpe and art viruses were obtained fr ... | 1992 | 1342498 |
| seroepidemiology of california and bunyamwera serogroup bunyavirus infections in humans in california. | several human populations in california were surveyed cross-sectionally and longitudinally for neutralizing antibodies to selected arthropod-borne bunyaviruses in the california and bunyamwera serogroups. overall, the prevalence of antibodies to california serogroup viruses was 6.4% in 702 individuals sampled during 1963-1988. comparative antibody titers in individual sera indicated that 4.1% and 1.6% of these infections were caused by viruses similar or identical to jamestown canyon and califor ... | 1992 | 1357960 |
| vector competence of california mosquitoes for california encephalitis and california encephalitis-like viruses. | mosquitoes collected from coastal, inland valley, and alpine locations in california were evaluated for their experimental vector competence for two viruses in the california serogroup (bunyaviridae:bunyavirus). aedes squamiger, a coastal salt marsh mosquito, was an efficient vector of a california encephalitis (ce)-like virus isolated from its habitat (89% of the pledget-fed females became infected and 61% transmitted virus). aedes dorsalis, a coastal mosquito, and ae. melanimon, an inland vall ... | 1992 | 1360192 |
| arbovirus isolations from mosquitoes collected during 1988 in the senegal river basin. | during august and september 1988, we collected adult mosquitoes from 14 locations in the senegal river basin to search for evidence of rift valley fever (rvf) viral activity one year after the 1987 outbreak, which occurred along the senegal-mauritania border. more than 62,000 specimens representing 18 species in seven genera were collected with carbon dioxide-baited, solid-state army miniature light traps and sheep-baited traps. twenty virus isolations from culex, aedes, and anopheles mosquitoes ... | 1992 | 1361722 |
| selective inhibition of arthropod-borne and arenaviruses in vitro by 3'-fluoro-3'-deoxyadenosine. | a novel nucleoside analog, 3'-fluoro-3'-deoxyadenosine (3'f3'dado), was evaluated for antiviral activity against several arthropod-borne and arenaviruses in vero cell culture. the following 50% inhibitory concentrations (ec50) of virus plaque formation were obtained against the test viruses: semliki forest (10.3 microm) and venezuelan equine encephalitis (5.3 microm) alphaviruses, lymphocytic choriomeningitis (7.7 microm) and pichinde (greater than 32 microm) arenaviruses, punta toro (greater th ... | 1992 | 1365816 |
| effect of macrophage source and activation on susceptibility in an age-dependent model of murine hepatitis caused by a phlebovirus, punta toro. | the adames strain of a bunyavirus, punta toro virus (ptv), is an hepatotrophic virus that has been described to produce an age-dependent lethal hepatic necrosis in 3-4 week old c57bl/6 mice, but 8 week old mice survive with minimal necrosis. the course of ptv infection in vitro in macrophages derived from these mice served as a model to study the pathogenesis of phlebovirus infection. peripheral blood monocytes, resident or elicited peritoneal macrophages, and kupffer cell liver macrophages, as ... | 1992 | 1370368 |
| organization of germiston bunyavirus m open reading frame and physicochemical properties of the envelope glycoproteins. | we describe the construction of plasmids which express fusion proteins representing various regions of germiston virus m polyprotein. the fusion proteins were purified and inoculated into rabbits to produce antisera. the n- and c-terminal regions of the polyprotein induced specific antibodies which reacted with glycoproteins g2 and g1, respectively, and the intermediate region induced antibodies against the nsm polypeptide. this enabled us to determine the gene order: g2-nsm-g1. glycoproteins g1 ... | 1992 | 1402815 |
| non-random reassortment between the tripartite rna genomes of la crosse and snowshoe hare viruses. | the process of reassortment between the tripartite rna genomes (segments designated l, m and s) of snowshoe hare and la crosse bunyaviruses (bunyaviridae) has been investigated by polymerase chain reaction analysis of greater than 250 progeny recovered at 72 h post-infection from dual wild-type virus infections involving high multiplicities (approximately 5) of each virus in a bhk-21 cell line. statistical analysis of the data indicated that rna segment reassortment was not random, and for these ... | 1992 | 1402816 |
| the nucleotide sequence of the m rna segment of tomato spotted wilt virus, a bunyavirus with two ambisense rna segments. | the complete sequence of the tomato spotted wilt virus (tswv) m rna segment has been determined. the rna is 4821 nucleotides long and has an ambisense coding strategy similar to that of the s rna segment. the m rna segment contains two open reading frames (orfs), one in the viral sense which encodes a protein with a predicted size of 33.6k, and one in the viral complementary sense which encodes the precursor to the g1 and g2 glycoproteins, with a predicted size of 127.4k. both orfs are expressed ... | 1992 | 1431808 |
| [gene structures of arenaviruses and bunyaviruses--ambisense rna viruses]. | 1992 | 1438820 | |
| simulation of arbovirus overwintering: survival of toscana virus (bunyaviridae:phlebovirus) in its natural sand fly vector phlebotomus perniciosus. | a series of experiments were done to study the effect of simulated summer and winter temperatures on the development of phlebotomus perniciosus (diptera:psychodidae) and on the survival of toscana virus in transovarially infected insects. sand flies maintained at 28 degrees c developed relatively fast, with adults emerging from 40 to 55 days after initial oviposition. similar results were obtained with insects reared at 25 degrees c. in contrast, sand flies maintained at 15 degrees c developed s ... | 1992 | 1449197 |
| synthesis and antiviral evaluation of n-carboxamidine-substituted analogues of 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamidine hydrochloride. | ten, hitherto unreported, analogues of 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamidine hydrochloride (2a, ribamidine) and methyl carboximidate 5 have been synthesized. these include the n-cyano (2b), n-alkyl (2c-e), n-amino acid (2f-h), n,n'-disubstituted (6, 7a,b), and the n-methylated carboxamide (1f) analogues of ribavirin. in addition, a new facile synthesis of carboxamidine 2a was also developed. all compounds were evaluated for biological activity against the following rna viruses: p ... | 1992 | 1507208 |
| association of the nonstructural protein nss of uukuniemi virus with the 40s ribosomal subunit. | the small rna segment (s segment) of uukuniemi (uuk) virus encodes two proteins, the nucleocapsid protein (n) and a nonstructural protein (nss), by an ambisense strategy. the function of nss has not been elucidated for any of the bunyaviruses expressing this protein. we have now expressed the n and nss proteins in sf9 insect cells by using the baculovirus expression system. high yields of both proteins were obtained. a monospecific antibody was raised against gel-purified nss and used to study t ... | 1992 | 1534850 |
| localization to the golgi complex of uukuniemi virus glycoproteins g1 and g2 expressed from cloned cdnas. | the membrane glycoproteins g1 and g2 of uukuniemi virus, a bunyavirus, accumulate in the golgi complex (gc) during virus infection. these proteins have therefore been considered to be good models for studying the intracellular transport to and retention in the gc. in this study, i have used indirect immunofluorescence to localize in cos cells the uukuniemi virus glycoproteins g1 and g2 expressed together or separately from cloned cdnas with use of simian virus 40-based vectors. when expressed to ... | 1992 | 1602557 |
| nucleotide sequence and coding strategy of the uukuniemi virus l rna segment. | the complete nucleotide sequence of the l rna segment of uukuniemi virus has been determined from cloned cdna. the l rna is 6423 nucleotides in length, and is of negative polarity. the viral-complementary rna contains a single large open reading frame of 2104 codons which corresponds to the l protein (m(r) 241039). comparison with the l protein sequences of other members of the bunyaviridae showed homology with the rift valley fever phlebovirus l protein (38% amino acid identity), but no detecta ... | 1992 | 1629699 |
| vector competence of selected mosquito species (diptera: culicidae) for california strains of northway virus (bunyaviridae: bunyavirus). | selected mosquito species from central valley, coastal, and alpine habitats of california were evaluated for their vector competence for northway (nor) virus. culiseta incidens thomson, culiseta inornata (williston), and anopheles freeborni aitken were the only competent vectors when fed virus. aedes sierrensis (ludlow), as well as alpine snow pool aedes (i.e., ae. cataphylla dyar, ae. hexodontus dyar, ae. increpitus dyar and ae. tahoensis dyar), ae. melanimon dyar, ae. washinoi lanzaro & eldrid ... | 1993 | 8099625 |
| detection of the l protein of tomato spotted wilt virus. | the 5'-terminal and 3'-terminal parts of the single open reading frame (orf) in the l rna of tomato spotted wilt virus (tswv) were expressed using a prokaryotic expression system. using antibodies raised against the translational products obtained a 330-kda protein could be specifically detected in preparations of purified virions and in nucleocapsid preparations from tswv-infected leaf tissue. the results obtained indicate that the l protein of tswv, though much larger than that of the animal-i ... | 1993 | 8212587 |
| molecular determinants of the virulence and infectivity of california serogroup bunyaviruses. | california bunyaviruses cause encephalitis in mammalian hosts after peripheral infection. the virulence of these viruses is determined by their ability to replicate sequentially in striated muscle, cause viremia, and invade and replicate in the central nervous system. these viruses are also able to infect vector mosquitoes following ingestion of a blood meal containing virus. bunyaviruses are negative stranded rna viruses with a trisegmented genome, and the large, medium, and small rna segments ... | 1993 | 8257095 |
| inhibitory effect of heparin on rhabdovirus and bunyavirus isolated from cattle. | 1993 | 8368970 | |
| non-viral sequences at the 5' ends of dugbe nairovirus s mrnas. | analyses of the mrna transcription processes of viruses in four genera (bunyavirus, hantavirus, phlebovirus and tospovirus) of the family bunyaviridae have revealed a common mechanism of initiation using host-derived primers, known as cap-snatching. to provide similar information on the fifth genus in the family, the 5' ends of dugbe nairovirus s mrna species were specifically cloned and sequenced. this revealed the presence of non-viral heterogeneous sequences, five to 16 nucleotides in length ... | 1993 | 8409954 |
| characterization of bunyamwera virus s rna that is transcribed and replicated by the l protein expressed from recombinant vaccinia virus. | analysis of the 5' termini of bunyamwera virus s segment mrnas by cloning and sequence analysis revealed the presence of nonviral, heterogeneous sequences 12 to 17 bases long. this is similar to reports for other members of the family bunyaviridae and is taken to indicate that mrna transcription is primed by a "cap-snatching" mechanism. the 3' end of the bunyamwera virus s mrna was mapped, by using an rnase protection assay, to 100 to 110 nucleotides upstream of the 3' end of the template. previ ... | 1993 | 8437222 |
| viruses of chelonia. | viruses occurring in turtles and tortoises are hetergeneous but according to ecologic characteristics and pathogenic properties they can be divided in two major groups: 1. arboviruses (toga-, flavi-, rhabdo- and bunyaviruses) transmitted by arthropods cause severe diseases in homoiothermic vertebrates. the viruses are of great epidemiological interest in human and veterinary medicine. chelonia and other reptiles infected by bites of vectors e.g. aedes, anopheles, culex develop cyclic viremia wit ... | 1993 | 8456570 |
| toscana virus genomic l segment: molecular cloning, coding strategy and amino acid sequence in comparison with other negative strand rna viruses. | the complete nucleotide sequence of toscana (tos) virus (bunyaviridae, phlebovirus) l segment was determined. the l segment is 6404 nucleotides long, containing a single open reading frame (orf) in the viral complementary sense coding for a protein of 2095 amino acids that, as in the case of negative strand rna viruses, could be part of the rna polymerase of tos virus. this orf is expressed by a messenger rna (mrna) as long as the genomic segment. like the mrnas expressed by the genomic segments ... | 1993 | 8460526 |
| characterization of murine caraparu bunyavirus liver infection and immunomodulator-mediated antiviral protection. | a rapid, peripheral disease model utilizing the bunyavirus, caraparu, was established in mice for the evaluation of antiviral therapy with immunomodulators. 4-6-week-old b6c3f1 female mice, inoculated intraperitoneally with virus, developed coagulative liver necrosis and died between 4-6 days after infection. this caraparu disease model was relatively resistant to treatment with immunomodulators, such as abmp, ampligen, alpha-interferon (ifn-alpha) or beta-interferon (ifn-beta). however, a signi ... | 1993 | 8460932 |
| the nucleotide sequence of the m rna segment of tomato spotted wilt virus, a bunyavirus with two ambisense rna segments. | 1993 | 8468562 | |
| polygenic control of neuroinvasiveness in california serogroup bunyaviruses. | the pathogenesis of the california serogroup bunyaviruses includes both extraneural and intraneural replicative phases that can be separated experimentally. the present study dissects the viral genetic determinants of extraneural replication. we have previously described two attenuated reassortant clones of california serogroup bunyaviruses which exhibit reduced neuroinvasiveness after subcutaneous inoculation into suckling mice. clone b1-1a bears an attenuated middle rna segment (neuroinvasiven ... | 1993 | 8510208 |
| isolation of batai virus (bunyaviridae:bunyavirus) from the blood of suspected malaria patients in sudan. | from august through november 1988, 77,500 patients with fever presented to the municipal hospital and to eight government health centers in kassala, a town of approximately 400,000 individuals in eastern sudan. a diagnosis of malaria, based primarily on clinical presentation, was made in 14,395 individuals during this four-month period; fevers of unknown origin were diagnosed in 29 patients. a bunyavirus that was antigenically similar or identical to batai virus by complement fixation and plaque ... | 1993 | 8517485 |
| medically important arboviruses of the united states and canada. | of more than 500 arboviruses recognized worldwide, 5 were first isolated in canada and 58 were first isolated in the united states. six of these viruses are human pathogens: western equine encephalitis (wee) and eastern equine encephalitis (eee) viruses (family togaviridae, genus alphavirus), st. louis encephalitis (sle) and powassan (pow) viruses (flaviviridae, flavivirus), lacrosse (lac) virus (bunyaviridae, bunyavirus), and colorado tick fever (ctf) virus (reoviridae, coltivirus). their scien ... | 1994 | 8118792 |
| the s rna genome segments of batai, cache valley, guaroa, kairi, lumbo, main drain and northway bunyaviruses: sequence determination and analysis. | bunyaviruses have a genome comprising three segments of negative-sense rna. the smallest rna segment, s, encodes the nucleocapsid protein, n, and a nonstructural protein, nss, in overlapping reading frames. the sequences of the s genome rna segments of seven bunyaviruses (batai, cache valley, guaroa, kairi, main drain, northway and lumbo) were determined from cloned cdnas obtained using a one-step reverse transcription-pcr protocol. these sequences were compared to those of six viruses previousl ... | 1994 | 8126455 |
| replication in cultured c2c12 muscle cells correlates with the neuroinvasiveness of california serogroup bunyaviruses. | the neuroinvasiveness of california serogroup bunyaviruses is determined by the ability of the virus to replicate in striated muscle after peripheral inoculation of mice. neuroinvasiveness was mapped to the medium (m) rna segment of the virus, which encodes the viral glycoproteins, when reassortants were made between la crosse/original virus, a neuroinvasive isolate, and tahyna-181/57 virus, a nonneuroinvasive clone. we have tested the murine muscle cell line c2c12 as a surrogate for myotropism ... | 1994 | 8184551 |
| viruses isolated from mosquitoes collected in sri lanka. | attempts to isolate viruses from 178,181 unengorged female mosquitoes collected from different ecologic areas of sri lanka yielded 31 isolates: 17 of japanese encephalitis (je) virus, nine of getah virus, three of a batai-related bunyavirus, and two of arkonam virus. culex tritaeniorhynchus and mansonia uniformis mosquitoes were found to carry je virus in a dry zone nonepidemic area, and cx. pseudovishnui was found to carry it in a wet zone nonepidemic area. japanese encephalitis virus was isola ... | 1994 | 7915499 |
| localization of bunyamwera bunyavirus g1 glycoprotein to the golgi requires association with g2 but not with nsm. | the bunyamwera bunyavirus (bun) m rna genome segment encodes three proteins, two glycoproteins termed g1 and g2 and a non-structural protein called nsm, in the form of a polyprotein precursor that is co-translationally cleaved to give the mature proteins. indirect immunofluorescence experiments have shown that these proteins localize to the golgi complex in bun-infected cells. we have used a recombinant vaccinia virus (vtf7-3), which expresses bacteriophage t7 rna polymerase, to drive the expres ... | 1994 | 7996137 |
| [the antiviral activity of inhibitors of adp ribosylation in experimental alpha- and bunyavirus infections]. | the prophylactic and therapeutic effects of adp-ribosylation inhibitors, 3,n-acetylaminobenzamide and 3,n-butyrylaminobenzamide, were studied in mice inoculated with an alphavirus or a bunyavirus. both drugs were shown to have high levels of antiviral activity when given as a single subcutaneous injection in a dose of 10 mg/kg while increasing the number of injections did not increase their efficacy or might even decrease it. | 1994 | 8017062 |
| arboviruses causing human disease in the australasian zoogeographic region. | over 65 arboviruses have been reported from countries in the australasian zoogeographic region, but only a few have been implicated in human disease. these include the flaviviruses murray valley encephalitis (mve), kunjin (kun), kokobera (kok), and dengue, particularly types 1 and 2; the alphaviruses ross river (rr), barmah forest (bf), and sindbis (sin); and the bunyaviruses, gan gan and trubanaman. in this paper recent epidemiological and clinical results pertaining to these viruses are review ... | 1994 | 8031248 |
| a signal for golgi retention in the bunyavirus g1 glycoprotein. | the g1 and g2 glycoproteins of punta toro virus, a member of the bunyaviruses, are targeted to the golgi complex, where viral budding occurs. we found that the g1 protein, when expressed in the absence of g2, is also targeted to the golgi complex. a series of g1 proteins truncated at the carboxyl-terminal region was constructed, and the localization of the expressed proteins was examined. it was found that the proteins expressed from constructs with partial deletions in the cytoplasmic domain we ... | 1994 | 8077205 |
| cache valley virus. | cache valley virus (cvv) is a causative agent of a mosquito-borne disease syndrome of sheep and, possibly, of all ruminants, characterized by embryonic and fetal death, stillbirths, and multiple congenital malformations. cvv is endemic in canada, mexico, and the united states. several related bunyaviruses also may play a role in syndromes of congenital malformations and embryonic losses in north america. | 1994 | 7728634 |
| akabane virus. | akabane virus, an arthropod-borne bunyavirus, is the major cause of epizootics of congenital malformations in ruminants in australia, japan, korea, and israel, and is suspected to be a cause of sporadic outbreaks elsewhere. blood-sucking insects, such as biting midges, transmit the virus horizontally to vertebrates. climatic factors influence the seasonal activity and geographic range of the vector population and, therefore, occurrence of related disease. inoculated ruminants seroconvert rapidly ... | 1994 | 7728635 |
| [nephropathia epidemica. hantavirus nephritis--a differential diagnosis in acute abdomen]. | nephropathia epidemica (ne) is an infectious disease caused by hantavirus of the bunyavirus family and carried by little rodents, in denmark presumably by the bank vole (clethrionomys glareolus). the disease usually presents with self-limiting renal failure, thrombocytopenia, fever, lower back and/or abdominal pain. as such it might be confused with for example acute abdomen as shown by the two cases given. final diagnosis is based upon demonstrating antibody formation against hantavirus. | 1994 | 7810017 |
| rift valley fever virus l segment: correction of the sequence and possible functional role of newly identified regions conserved in rna-dependent polymerases. | the sequence of rift valley fever virus l segment that we published in a previous paper was erroneous in the 3'-terminal region of the antigenomic rna molecule. here, we have shown that the l segment is in fact 6404 nucleotides long and encodes a polypeptide of 237.7k in the viral complementary sense. sequence comparisons performed between the rna-dependent rna polymerases of 22 negative-stranded rna viruses revealed the existence of two novel regions located at the amino termini of the proteins ... | 1994 | 7515937 |
| transcription of a recombinant bunyavirus rna template by transiently expressed bunyavirus proteins. | we describe a convenient system for analyzing bunyavirus transcription using a recombinant rna template derived from the plasmid pbunscat, which comprises a negative-sense reporter gene (chloramphenicol acetyltransferase or cat) flanked by the exact 5' and 3' untranslated regions of the bunyamwera virus (bun) s rna segment. when cells which expressed bunyavirus proteins (either by recombinant vaccinia viruses or by the vaccinia virus-t7 system) were transfected with bunscat rna, cat activity cou ... | 1995 | 7544044 |
| [searching for hepatitis c virus by immunoelectron microscopy and its morphology]. | many investigators have searched for the causative agent(s) of blood-borne non-a, non-b hepatitis in sera and liver from patients and experimental animals, and have reported various virus-like particles, morphologically resembling parvovirus, togavirus, picornavirus, hepadnavirus, paramyxovirus, papovavirus, retrovirus or bunyavirus. despite extensive effort, none of these virus-like particles has been confirmed universally as an etiologic virus because of the absence of immunological identifica ... | 1995 | 7563652 |
| effect of heparin on hemagglutination by akabane and aino viruses belonging to the simbu group of bunyaviruses. | heparin inhibited the hemagglutinin activity of akabane and aino viruses. the minimal inhibitory concentration of heparin required to inhibit 8 hemagglutination (ha) u of akabane and aino viruses was 10 u/ml. goose erythrocytes failed to combine with the ha inhibitory factor of heparin. on the other hand, goose erythrocytes treated with heparinase had greatly reduced agglutinability by akabane virus. virus-heparin complex formation was observed by sedimenting heparin with the virus particles. | 1995 | 7571376 |
| isolation of potosi virus from aedes albopictus in north carolina. | a total of 4,169 adult mosquitoes were aspirated from 3 tire disposal sites in north carolina for virus assays. aedes albopictus was the dominant species, with a relative abundance of approximately 99%. potosi virus was isolated from one pool of 68 female ae. albopictus. priorities for future potosi virus research and the implications of the north carolina isolate are discussed. | 1995 | 7595449 |
| homodimeric association of the spike glycoproteins g1 and g2 of uukuniemi virus. | we have studied the interactions of the g1 and g2 membrane glycoproteins of uukuniemi virus, a bunyavirus, in virus particles and in triton x-100-solubilized virus. the g1 glycoprotein in intact virus or in triton solution could be oxidized into a covalent homodimer using cu2+ ion as a catalyst. immunoprecipitations of the glycoproteins from triton-solubilized virus lysates showed that g1 and g2 do not form a stable heterodimeric or heterooligomeric complex. the oligomeric association of g1 and ... | 1995 | 7645217 |
| bluetongue and douglas virus activity in new south wales in 1989: further evidence for long-distance dispersal of the biting midge culicoides brevitarsis. | 1995 | 7661825 | |
| [mosquito-borne arboviruses in the baikal region]. | a total of 20 virus strains were isolated from arthropods and rodents in the baikal region in 1992. thirteen of these were california serogroup viruses, 4 batai virus strains (bunyaviridae, bunyavirus), and 3 gheta virus strains (togaviridae, alphavirus, semliki complex). the rates of mosquito infection with california, batai, and gheta viruses were 1:6500, 1:24000, and 1:24000, respectively. the results of the neutralization test indicate the similarity of the isolated california complex strain ... | 1995 | 7483568 |
| completion of the la crosse virus genome sequence and genetic comparisons of the l proteins of the bunyaviridae. | la crosse virus is a member of the bunyavirus genus in the family bunyaviridae, viruses with trisegmented rna genomes of mostly negative polarity composed of large (l), medium (m), and small (s) segments. the sequences of the la crosse/original m and s rna segments have been previously characterized. using reverse transcriptase in conjunction with pcr amplification, we have obtained the nucleotide sequence of the l rna segment, which encodes the viral polymerase in a single large open reading fr ... | 1995 | 7831835 |
| an rna-dependent rna polymerase activity associated with virions of tomato spotted wilt virus, a plant- and insect-infecting bunyavirus. | an rna-dependent rna polymerase activity was found associated with virions of tomato spotted wilt virus (tswv), a plant- and insect-infecting member of the family bunyaviridae. radiolabeled nucleoside triphosphates were incorporated into trichloroacetic acid-precipitable products by detergent-disrupted, purified tswv virions. incorporation was reduced to near-background levels when rnase was present in the reaction mixture. the predominantly double-stranded rna products were rnase-resistant at h ... | 1995 | 7871744 |
| determination and comparative analysis of the small rna genomic sequences of california encephalitis, jamestown canyon, jerry slough, melao, keystone and trivittatus viruses (bunyaviridae, genus bunyavirus, california serogroup). | the nucleotide sequences of the small (s) genomic rnas of six california (cal) serogroup bunyaviruses (bunyaviridae: genus bunyavirus) were determined. the s rnas of two california encephalitis virus strains, two jamestown canyon virus strains, jerry slough virus, melao virus, keystone virus and trivittatus virus contained the overlapping nucleocapsid (n) and non-structural (nss) protein open reading frames (orfs) as described previously for the s rnas of other cal serogroup viruses. all n prote ... | 1995 | 7897347 |
| protection from la crosse virus encephalitis with recombinant glycoproteins: role of neutralizing anti-g1 antibodies. | la crosse virus, a member of the california serogroup of bunyaviruses, is an important cause of pediatric encephalitis in the midwestern united states. like all bunyaviruses, la crosse virus contains two glycoproteins, g1 and g2, the larger of which, g1, is the target of neutralizing antibodies. to develop an understanding of the role of each of the glycoproteins in the generation of a protective immune response, we immunized 1-week-old mice with three different preparations: a vaccinia virus re ... | 1995 | 7745694 |
| bluetongue and douglas virus activity in new south wales in 1989: further evidence for long-distance dispersal of the biting midge culicoides brevitarsis. | infection of cattle with bluetongue and douglas viruses was detected on the central and southern coast of new south wales from january to april 1989. bluetongue virus infection was found well south of areas of expected occurrence. evidence is presented to support wind-borne dispersal of infected vectors, culicoides brevitarsis, southwards from the hunter valley. | 1995 | 7779035 |
| tropism of bunyaviruses: evidence for a g1 glycoprotein-mediated entry pathway common to the california serogroup. | the california serogroup is composed of antigenically and biologically related viruses within the bunyavirus genus of the bunyaviridae. we used a large panel of murine cells to study their tissue tropisms and found virtually identical patterns of viral replication among all of the members of this serogroup, in contrast to other members of the family (bunyamwera, cache valley, and punta toro viruses). by analyzing the nonpermissive infections with both an rna dot-blot and a virus binding assay, w ... | 1995 | 8553534 |
| hantaviruses: clinical, microbiologic, and epidemiologic aspects. | hantaviruses comprise a genus of the family bunyaviridae. bunyaviruses are enveloped viruses with a negative-sense, tripartite rna genome. hantaviruses are etiologic agents for two acute and severe illnesses of man, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). each hantavirus is primarily associated with a single rodent host species or genus, and is transmitted to man through accidental inhalation or ingestion of virus-contaminated rodent excreta. the dis ... | 1995 | 8561891 |
| typing of lacrosse, snowshoe hare, and tahyna viruses by analyses of single-strand conformation polymorphisms of the small rna segments. | single-strand conformation polymorphism analysis was developed to differentiate the small rna segments of three california serogroup bunyaviruses. the small rna segments of la crosse, snowshoe hare, and tahyna viruses were reverse transcribed and pcr amplified. the cdnas were then denatured, rapidly chilled to promote intrastrand reassociation, separated electrophoretically on a nondenaturing gel at room temperature, and silver stained. the resulting single-strand conformation polymorphism patte ... | 1995 | 8586697 |
| arbovirus surveillance in italy. | a high number of different arboviruses have been demonstrated to be present in italy, due to the coexistence of climates that are characteristic of continental areas in the north of the country and of subtropical areas in the south. viruses circulating in central europe, such as the tick-borne encephalitis (tbe) virus, are present in central and northern regions, whereas viruses circulating in mediterranean areas, such as the sandfly fever viruses are present in central and southern italy. virus ... | 1995 | 8778650 |
| interactions between the ibv nucleocapsid protein and rna sequences specific for the 3' end of the genome. | the infectious bronchitis virus (ibv) nucleocapsid protein was expressed as a fusion protein in bacteria. the coding sequence differed from the native protein only in the addition of six histidine residues at the amino terminus which were used for enrichment with a nickel affinity column. in gel shift assays, the mobility of labelled g rna was decreased with increasing concentrations of the fusion protein. competitive gel shift assays with labelled g rna indicated that the protein interacted wit ... | 1995 | 8830535 |
| california encephalitis in alabama. | arthropod-borne virus (arbovirus) infections in humans are primarily central nervous system infections, but other clinical manifestations include febrile illness and fever with hemorrhagic diathesis. in the genus bunyavirus there are several viruses that cause disease in humans, especially in north america; these include lacrosse, jamestown canyon, trivittatus, and snowshoe hare viruses. the disease seen mainly in children is california encephalitis (usually of the lacrosse subtype); this infect ... | 1996 | 8865793 |
| the extracellular domain of la crosse virus g1 forms oligomers and undergoes ph-dependent conformational changes. | the la crosse virus g1 glycoprotein plays a critical role in virus binding to susceptible cells and in the subsequent fusion of viral and cellular membranes. a soluble form of the g1 glycoprotein (sg1) prepared in a recombinant baculovirus system mimics the cell-binding pattern of la crosse virus and inhibits la crosse virus infection (a. pekosz et al., virology 214, 339-348, 1995), presumably by competing for a cellular receptor, a finding that implies that sg1 can perform some functions absent ... | 1996 | 8918555 |
| [arbovirus circulation in the republic of guinea]. | in 1978-1991 the ussr-guinea virological and microbiological laboratory functioned in kindia, the republic of guinea. arbovirus activity in this country was studied by a number of virologists and other specialists. their personal contribution and achievements in this collaboration are reflected in the present paper. about 74,000 mosquitoes, 100,000 ixodidae ticks, 1,500 wild birds, 2,700 bats, 106 monkeys, and 308 other mammals, 927 blood samples collected from febrile patients were examined in ... | 1996 | 8926934 |
| molecular determinants of golgi retention in the punta toro virus g1 protein. | the g1 glycoprotein of punta toro virus, a member of the bunyavirus family, accumulates in the golgi complex when it is expressed from cloned cdna. we previously reported that the information necessary for golgi retention of the g1 protein is located within the transmembrane domain and a portion of the cytoplasmic domain adjacent to the transmembrane domain (matsuoka, y., chen, s.-y., and compans, r. w. (1994) j. biol. chem. 269, 22565-22573). to determine the features of the amino acid sequence ... | 1996 | 8951050 |
| rescue of a segmented negative-strand rna virus entirely from cloned complementary dnas. | we provide the first report, to our knowledge, of a helper-independent system for rescuing a segmented, negative-strand rna genome virus entirely from cloned cdnas. plasmids were constructed containing full-length cdna copies of the three bunyamwera bunyavirus rna genome segments flanked by bacteriophage t7 promoter and hepatitis delta virus ribozyme sequences. when cells expressing both bacteriophage t7 rna polymerase and recombinant bunyamwera bunyavirus proteins were transfected with these pl ... | 1996 | 8986823 |
| use of the yellow fever virus vaccine strain 17d for the study of strategies for the treatment of yellow fever virus infections. | we have employed the attenuated vaccine strain 17d of yellow fever virus (yfv) to evaluate the inhibitory effect of a selected series of compounds on yfv in vero cells. use of the vaccine strain does not require high-level microbiological containment facilities and should allow extensive screening. in addition, yfv may serve as a model for other flaviviruses including hepatitis c virus (hcv), and thus strategies for the treatment of yfv infections may apply to flavivirus infections in general. i ... | 1996 | 8783804 |
| sandfly fever viruses in italy. | two serologically distinct agents, the sandfly fever sicilian and the sandfly fever naples viruses, were isolated by sabin from blood samples taken during an italian epidemic of febrile illness. since then, several different viruses have been isolated from sandflies and/or humans in both the old and new world. toscana virus, a new virus closely antigenically related to sandfly fever naples virus, was isolated in 1971 from the sandfly phlebotomus perniciosus in italy. extensive studies on the imp ... | 1996 | 8800804 |
| detection of california serogroup bunyaviruses in tissue culture and mosquito pools by pcr. | a reverse transcription-polymerase chain reaction (rt-pcr) protocol was developed for rapid detection of 14 california serogroup viruses using universal primer pairs. these primers are specific for the small rna (s rna) and middle rna (m rna) segments. the method has been employed to detect jamestown canyon (jc) virus in naturally infected mosquitoes. with this technique, it is possible to detect virus in an amount of material equivalent to as little as 0.03 mosquito. | 1996 | 8801229 |
| antiviral activities of tragacanthin polysaccharides on punta toro virus infections in mice. | tragacanthin polysaccharides from astragalus brachycentrus (av208) and astragalus echidnaeformis (av212) plants, which are devoid of in vitro antiviral activity, were evaluated in a mouse model of punta toro virus (ptv) infection. the ptv (a phlebovirus member of the bunyaviridae family of viruses) is a model for studying the treatment of rift valley fever and hantavirus infections. single intraperitoneal treatments with 12.5-200 mg/kg/day doses of av212 given 24 h before or 4 and 24 h after vir ... | 1996 | 8804797 |
| the complete nucleotide sequence and genome organization of the m rna segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. | the m rna of peanut bud necrosis virus (pbnv; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. it comprised two orfs in an ambisense organization and terminal inverted repeats. the 3' large orf (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kda which was identified as the glycoprotein precursor (gp) of the g1 and g2 glycoproteins. a comparison of the deduced amino acid sequence of gp revealed 37% identity and 58-59% simil ... | 1996 | 8811036 |
| the role of deer as a possible reservoir host of potosi virus, a newly recognized arbovirus in the united states. | potosi (pot) virus (bunyaviridae) was isolated from aedes albopictus, an introduced asian mosquito species, collected at a used tire yard in potosi, missouri (usa), in august and september, 1989. in september, 1990, small animals were trapped at the tire yard and six cattle were sampled at an adjacent farm; in november 1990 and 1991, blood samples were collected with filter paper strips from 364 hunter-killed, white-tailed deer (odocoileus virginianus) in the region to determine the possible res ... | 1996 | 8827670 |
| medical microbiology | bunyaviridae is a family of arthropod-borne or rodent-borne, spherical, enveloped rna viruses. bunyaviruses are responsible for a number of febrile diseases in humans and other vertebrates. they have either a rodent host or an arthropod vector and a vertebrate host. | 1996 | 21413286 |
| the infectious bronchitis virus nucleocapsid protein binds rna sequences in the 3' terminus of the genome. | the infectious bronchitis virus (ibv) nucleocapsid protein was expressed as a bacterial fusion protein which differed from the native protein only in the addition of six amino terminus histidine residues. using rna overlay protein blot assays, the recombinant protein was shown to bind to rna fragments specific for the positive sense 3' noncoding end of the ibv genome. at greater concentrations of sodium chloride, the native and fusion nucleocapsid proteins similarly bound to g rna, representing ... | 1996 | 8599203 |