Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| location of bovine respiratory syncytial virus antigens in the lung of experimentally-infected lambs: comparative study using indirect fluorescent antibody test, avidin-biotin-peroxidase complex and transmission electron microscopy. | the experiments were designed to analyse the efficiency of the indirect fluorescent antibody test (ifat), the avidin-biotin-peroxidase complex (abpc) and transmission electron microscopy (tem) in detecting bovine respiratory syncytial virus (brsv) antigen in ovine lung. 25 gnotobiotic merino lambs each received an intratracheal injection of 20 ml of brsv infected cell culture suspension (strain nmk-7) diluted at the concentration of 1.26 x 10(6) tcid50/ml. results showed that the immunological t ... | 1993 | 7682871 |
| [bovine serum albumin inhibits the adsorption of respiratory syncytial virus on mdbk cells]. | in order to obtain optimal bovine respiratory syncytial virus adsorption to host cells, the effect of several products (fetal bovine serum, bovine serum albumin, ovalbumin and cytochrome c) was studied. the adsorption of the virus to mdbk cells was higher in the presence of 2% than in the presence of 5% fetal bovine serum. adsorption was inhibited in the presence of bovine serum albumin at concentrations > 0.2% when added before or at the same time as the virus. ovalbumin and cytochrome c did no ... | 1993 | 8111432 |
| morbidity and mortality in a large irish feedlot; microbiological and serological findings in cattle with acute respiratory disease. | a survey of morbidity and mortality was undertaken in a slatted unit containing 6399 beef cattle over a 6 month period. the mortality rate was 0.78% and the morbidity rate was 12.7%. the interval from arrival to morbidity was 25.5 days +/- 0.9. significantly more sick cattle were identified on either mondays or tuesdays than were seen on saturdays or sundays (p < 0.005). the mean interval to mortality (all diseases) was 48.5 days +/- 7.4. respiratory disease was the most frequently recorded dise ... | 1993 | 8111615 |
| antigenic diversity of respiratory syncytial viruses and its implication for immunoprophylaxis in ruminants. | bovine respiratory syncytial virus (brsv) is a very important pathogen of cattle and perhaps other ruminants. it is a major contributor to the incidence of respiratory tract disease in nursing beef and feedlot and dairy calves. the genome of respiratory syncytial viruses encodes 10 proteins translated from 10 unique mrnas. the major glycoprotein (g), fusion protein (f), 1a protein and the 22k protein are components of the viral envelope. the nucleocapsid contains the nucleocapsid protein (n), th ... | 1993 | 8116189 |
| the immunologic response to experimental infection with bovine respiratory syncytial virus in lambs. | an experimental model was designed to study the humoral immune response in lambs experimentally infected with bovine respiratory syncytial virus (brsv), 25 merino lambs of both sexes, with a live weight of 17 +/- 3 kg, received an intratracheal inoculation of 20 ml. saline solution containing 1.26 x 10(6) tcid50 brsv (strain nmk-7) per ml. blood samples for serological analysis were collected from the jugular vein of the animals on days 0, 1, 3, 5, 7, 9, 11 and 15 post inoculation. serum protein ... | 1993 | 8128799 |
| associations between viral infections and respiratory disease in artificially reared calves. | market-purchased, week-old, dairy bred calves entering a commercial calf-rearing unit were blood sampled at six-week intervals until three months old. viral infections were monitored by elisa for antibodies to bovine herpesvirus 1, bovine respiratory syncytial virus, parainfluenzavirus-3, bovine adenovirus subgroup 1 and bovine viral diarrhoea virus (bvdv). the immunoperoxidase test was used to detect bvdv in serum. the total immunoglobulin concentration in the initial blood sample was measured ... | 1993 | 8212494 |
| structure and sequence comparison of bovine respiratory syncytial virus fusion protein. | the fusion (f) proteins of 10 strains of bovine respiratory syncytial virus (brsv) were compared by radioimmunoprecipitation with fractionation on sds-polyacrylamide gels. two different molecular weights (15 kda and 20 kda) of the f2 proteins were demonstrated among the brsv strains tested. to delineate the molecular basis for differences in the molecular weights of f2 subunits among the brsv strains, the nucleotide sequences of the f genes of fs1 and vc464 strains were determined from cdna clon ... | 1993 | 8212860 |
| dynamics of bovine respiratory syncytial virus infections: a longitudinal epidemiological study in dairy herds. | to study the epidemiology of respiratory syncytial virus (rsv) infections during the year, the incidences of primary infections and reinfections were monitored by titrating antibodies to bovine rsv (brsv) in cattle above 2 months of age in 6 dairy herds in the netherlands. from august 1990 until september 1991, 884 cattle were sampled at one-month intervals. a total of 155 cattle, most under two years of age, had a primary antibody response. antibody rises were found in 259 cattle of all ages. t ... | 1993 | 8257292 |
| epidemiological study of enzootic pneumonia in dairy calves in saskatchewan. | a field study involving 325 calves from 17 dairy herds in saskatchewan was conducted to determine the risk of enzootic pneumonia and to assess its association with a number of factors. two different case definitions of pneumonia were used in the analyses: the first was based on producers' treatment risk (case1) and the second was based on semimonthly clinical examinations of calves by the research veterinarian (case2). the risk of pneumonia based on case1 was 39% and on case2 was 29%. the measur ... | 1993 | 8269363 |
| structural difference in the fusion protein among strains of bovine respiratory syncytial virus. | the polypeptides of different strains of bovine respiratory syncytial virus (rsv) were compared. altered electrophoretic migrations were observed in the g, f, p, m and 22 kda polypeptides. the molecular weight of the f2 fragment in human rsv (long strain) and bovine rsv (a51908 and md-x strains) was approximately 20 kda whereas it was approximately 15.5 kda in caprine rsv and bovine rsv (fs-1 and vc-464 strains). the size difference of the f2 subunit was due to difference in the extent of glycos ... | 1993 | 8273280 |
| analysis of the ovine respiratory syncytial virus (rsv) g glycoprotein gene defines a subgroup of ungulate rsv. | respiratory syncytial virus (rsv) has been isolated from sheep suffering from respiratory tract disease. since the greatest differences between bovine rsv and human rsv are found on the attachment g protein, we have determined the nucleotide and deduced amino acid sequences of the g gene of ovine rsv. the latter contained 838 nucleotides and had a major open reading frame encoding a protein of 263 residues, and shared 73% nucleotide sequence identity with that of bovine rsv. the deduced amino ac ... | 1993 | 8277288 |
| reliable confirmation of antibodies to bovine respiratory syncytial virus (brsv) by enzyme-linked immunosorbent assay using brsv nucleocapsid protein expressed in insect cells. | the nucleocapsid (n) protein of bovine respiratory syncytial virus (brsv) in the baculovirus expression system was evaluated as a source of antigen in an enzyme-linked immunosorbent assay (elisa) for the detection of respiratory syncytial virus (rsv) antibodies. the recombinant n protein was purified from infected-cell extracts by sucrose gradient centrifugation and used in the elisa for the detection of antibodies to various rsv strains. the elisa was compared with the virus neutralization (vn) ... | 1993 | 8308106 |
| nucleotide sequence analysis of the ovine respiratory syncytial virus g glycoprotein gene. | respiratory syncytial virus (rsv) infects humans and animals including ruminants. among the 10 genes coded for in the viral genome, the putative attachment glycoprotein g gene has been the most variable among strains. human rsv have been divided to two subgroups based on immunological and base sequence data on the attachment glycoprotein g and its gene, respectively. it has been suggested that similar antigenic diversity also exists among bovine rsv (brsv) isolates. in this study, we report on t ... | 1993 | 8372453 |
| characteristic differences in reverse transcription-polymerase chain reaction products of ovine, bovine, and human respiratory syncytial viruses. | in reverse transcription-polymerase chain reactions (rt-pcr) and dna hybridizations using primers and an oligonucleotide probe to the fusion (f) protein mrna of bovine respiratory syncytial virus (brsv), all the brsv isolates and a goat isolate could be distinguished from prototype isolates of human respiratory syncytial viruses (hrsv) and ovine (sheep and bighorn sheep) respiratory syncytial viruses (rsv). however, rt-pcr amplifications with primers to sequences of the hrsv f protein mrna resul ... | 1993 | 8373845 |
| sequence variability of the glycoprotein gene of bovine respiratory syncytial virus. | sequence variation in the attachment glycoprotein g of bovine respiratory syncytial virus (brsv) was determined. the nucleotide sequences of the g mrnas of the a51908, vc464 and fs-1 strains of brsv were compared with the published sequence of the brsv strain 391-2. nucleotide sequence alignment showed that overall they are highly conserved, with 90 to 97% identity. in addition, the coding region of strain a51908 was longer by 18 nucleotides at the 3' end. an 84 to 95% level of identity was obse ... | 1993 | 8376974 |
| recent advances in bovine vaccine technology. | a description of new commercial and experimental vaccines for viral and bacterial diseases of cattle can be broadly divided into those used for both beef and dairy cows and those used predominantly in dairy cattle. for both types of cattle, newer and experimental vaccines are directed against several of the important viral (e.g., bovine herpesvirus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, parainfluenza type 3, and foot-and-mouth disease virus) and bacterial pathogens ( ... | 1993 | 8408872 |
| bovine respiratory syncytial virus protects cotton rats against human respiratory syncytial virus infection. | human respiratory syncytial virus (hrsv) is the most frequent cause of severe respiratory infections in infancy. no vaccine against this virus has yet been protective, and antiviral drugs have been of limited utility. using the cotton rat model of hrsv infection, we examined bovine respiratory syncytial virus (brsv), a cause of acute respiratory disease in young cattle, as a possible vaccine candidate to protect children against hrsv infection. cotton rats were primed intranasally with graded do ... | 1993 | 8437227 |
| bovine respiratory syncytial virus nucleocapsid protein expressed in insect cells specifically interacts with the phosphoprotein and the m2 protein. | the coding region of the gene for the nucleocapsid (n) protein of bovine respiratory syncytial virus has been inserted into the genome of autographa californica nuclear polyhedrosis virus (acnpv) using transfer vector pvl 1393. infection of spodoptera frugiperda cells with recombinant virus resulted in the synthesis of high levels of n protein. this protein was indistinguishable from the authentic bovine respiratory syncytial virus (brsv) n protein by sds-gel electrophoresis and immunoprecipitat ... | 1993 | 8438581 |
| identifying bovine respiratory syncytial virus by reverse transcription-polymerase chain reaction and oligonucleotide hybridizations. | an assay to identify tissue culture cells infected with bovine respiratory syncytial virus (brsv) that utilizes reverse transcription (rt), the polymerase chain reaction (pcr), and a synthetic oligonucleotide hybridization probe has been developed. the rt-pcr assay uses a brsv-specific negative-sense oligonucleotide primer to synthesize cdna from a brsv fusion protein mrna template and another brsv-specific oligonucleotide primer (positive sense) upstream from the negative-sense primer for pcr a ... | 1993 | 8501223 |
| assembly of enveloped respiratory syncytial virus particles within the cytoplasm of infected vero cells. | electron microscopic examination of syncytia induced by a bovine respiratory syncytial virus strain in vero cell cultures revealed the presence in the cytoplasm of assembled enveloped virus particles within inclusions of variable sizes. moreover, budding virus particles were shown occasionally in the intracytoplasmic vesicles. these particles were filamentous in form, about 80-120 nm in diameter, variable in length, containing 12 nm diameter nucleocapsids, and looked like the extracellular parti ... | 1994 | 8279956 |
| interaction of bovine respiratory syncytial virus with bovine alveolar macrophages in vivo: effects of virus infection upon selected cell functions. | the effect of bovine respiratory syncytial virus (brsv) upon alveolar macrophage (am) function was investigated using an in vivo calf inoculation model. alveolar macrophages were collected sequentially from live calves at multiple time points during the 14 day period following viral inoculation. alveolar macrophages from bronchoalveolar lavage fluids were purified by density gradient centrifugation (> 95% am) prior to in vitro evaluation of cell functions. there were significant but variable and ... | 1994 | 8143252 |
| infected cell types in ovine lung following exposure to bovine respiratory syncytial virus. | sixteen adult sheep (ten females, six males obtained from a closed flock at national animal disease center, ames, ia) were experimentally infected with bovine respiratory syncytial virus strain 375 (brsv), and lung tissues were stained for viral antigen. two infected sheep were euthanatized at each of the following post-inoculation times: 12, 24, 36, 48, 72, 96, 144, and 192 hours. lung, nasal turbinates, trachea, right cranial bronchial and mediastinal lymph nodes, liver, and spleen were collec ... | 1994 | 8203086 |
| field study of undifferentiated respiratory disease in housed beef calves. | a severe outbreak of undifferentiated respiratory disease affecting 119 of 144 (82.6 per cent) two- to five-month-old housed beef calves was studied by monitoring their clinical signs and rectal temperatures daily or every second day for two months. new cases of respiratory disease, which were first identified three weeks after the calves were housed, occurred over a period of 29 days. the cause of the outbreak was not conclusively determined although 20 per cent of the calves sampled showed ser ... | 1994 | 8203107 |
| antigenic heterogeneity of the attachment protein of bovine respiratory syncytial virus. | a panel of 23 monoclonal antibodies (mabs) specific for the attachment (g) glycoprotein of bovine respiratory syncytial virus (brs virus), recognizing seven antigenic areas on the g protein, was used to determine the antigenic heterogeneity among 19 brs viruses isolated over a 20 year period from various parts of the world. the pattern of reactivity of the isolates, as determined by elisa, identified two major subgroups of brsv. this finding was confirmed by radioimmunoprecipitation of the g pro ... | 1994 | 8113757 |
| nucleotide and predicted amino acid sequence analysis of the ovine respiratory syncytial virus non-structural 1c and 1b genes and the small hydrophobic protein gene. | respiratory syncytial virus (rsv) infects humans and cattle causing serious respiratory tract disease in both. the genome of the human and bovine rsv (hrsv and brsv) codes for two non-structural and eight structural proteins. rsv has also been isolated from naturally infected sheep, but the genome of the ovine rsv (orsv) has not been characterized and nor has the virus host range been identified. here we report on the cloning and sequencing of the two non-structural 1c and 1b genes and of the sm ... | 1994 | 8113762 |
| smoke and viral infection cause cilia loss detectable by bronchoalveolar lavage cytology and dynein elisa. | the purpose of this study was to quantitate cilia loss following airway epithelial cell injury. two models of airway injury were used: (1) ex vivo acute cigarette smoke exposure model: bovine lungs, obtained directly after slaughter, were ventilated with air or cigarette smoke for 5 min followed immediately by bronchoalveolar lavage (bal). the bronchi were examined histologically and bronchial and alveolar fractions of bal fluid were examined for cell counts, cell differentials, and cilia dynein ... | 1994 | 8111584 |
| double blind field evaluation of a trivalent vaccine against respiratory disease in veal calves. | a field trial was performed to determine the effect of a trivalent vaccine on clinical respiratory tract problems (crtp) in veal calves. the vaccine has been developed to increase immunity against the causal agents of ibr, bvd and brsv infection. in total 928 calves, housed in 16 compartments of one herd, were involved. in four compartments of 58 calves each, vaccinated and non-vaccinated calves were housed together. four other compartments were treated as a whole and 8 compartments were left un ... | 1994 | 7871698 |
| cytotoxic t cell responses in lambs experimentally infected with border disease virus. | mononuclear cells cytotoxic to a noncytopathic strain of border disease virus (bdv) but not to uninfected cells were detected in the peripheral blood of experimentally infected lambs, 10 days after experimental infection, reaching peak levels of cytotoxicity 15 days post-inoculation. the specificity of the cytotoxic activity to bdv-infected cells was tested by including autologous targets infected with another virus (bovine respiratory syncytial virus, brsv) and normal uninfected autologous targ ... | 1994 | 7941304 |
| spontaneous bovine respiratory syncytial virus infection in goats: pathological findings. | this paper presents the results of a study of eight cases of natural bovine respiratory syncytial virus (brsv) infection in a herd of 50 murciana kids from a intensive goat-farm in spain. clinical and pathological analysis confirmed the existence of natural brsv infection in eight kids. in two of the eight animals there was also evidence of concurrent infection with p. haemolytica a. | 1994 | 7941843 |
| effect of bovine respiratory syncytial virus infection on hypersensitivity to inhaled micropolyspora faeni. | respiratory syncytial virus causes mild-to-severe respiratory disease in human infants and young children; a closely related bovine respiratory syncytial virus causes a similar disease pattern in calves. increased disease severity in atopic children suggests that allergic reactivity may enhance the severity of rsv-induced disease. to examine the association between bovine respiratory syncytial virus (brsv) infection and allergic reactivity two groups of calves were exposed to aerosolized micropo ... | 1994 | 7950409 |
| the effect of a combined pasteurella haemolytica and haemophilus somnus vaccine and a modified-live bovine respiratory syncytial virus vaccine against enzootic pneumonia in young beef calves. | 1994 | 8076280 | |
| respiratory syncytial virus infections in human beings and in cattle. | respiratory syncytial virus (rsv) causes yearly outbreaks of respiratory disease in human beings and cattle all over the world. most severe human respiratory syncytial virus (hrsv)-associated disease is observed in children less than 1 year of age while most severe bovine respiratory syncytial virus (brsv)-associated disease is observed in calves less than 6 months of age. two subgroups of hrsv have been identified. the existence of two subgroups of brsv has been repeatedly suggested but is not ... | 1994 | 7806887 |
| development of nested pcr assays for detection of bovine respiratory syncytial virus in clinical samples. | two nested pcr assays were developed for the detection of bovine respiratory syncytial virus (brsv). primers were selected from the gene encoding the f fusion protein (pcr-f) and the gene encoding the g attachment protein (pcr-g). biotinylated oligonucleotide probes, termed f and g, were selected for the hybridization of the respective pcr products. the sensitivities of the pcr-f and pcr-g assays were similar, both detecting 0.1 tissue culture infective dose of the virus. the pcr-f assay amplifi ... | 1994 | 7814551 |
| [development of pcr tests for the detection of bovine herpesvirus-1, bovine respiratory syncytial viruses and pestiviruses]. | the development of pcr assays for detection of bhv-1, brsv, bvdv and another pestiviruses is summarized. a polymerase chain reaction assay based on primers selected from the viral gi glycoprotein gene detected 3 fg pure bhv-1 dna, 0.1-1.0 tcid50 or a single infected cell. no amplification was observed with dna from bhv-2, bhv-3, bhv-4, ohv-1 or ohv-2. however, a fragment of the correct size (468 bp) was amplified using dna from herpesviruses isolated from reindeer, red deer and goat. the pcr ass ... | 1994 | 7817501 |
| nucleotide sequence analysis of the non-structural ns1 (1c) and ns2 (1b) protein genes of bovine respiratory syncytial virus. | the nucleotide and deduced amino acid sequences of the non-structural (ns) protein genes of bovine respiratory syncytial virus (brsv) strain a51908 were determined. the ns1 and ns2 genes were 524 and 489 nucleotides long with single open reading frames, encoding polypeptides of 136 and 124 amino acids, respectively. comparison of the ns1 gene of brsv with the corresponding sequences of ovine respiratory syncytial virus (orsv) and human respiratory syncytial virus (hrsv) subgroups a and b reveale ... | 1995 | 7844532 |
| a seroepidemiological study of the importance in cow-calf pairs of respiratory and enteric viruses in beef operations from northwestern quebec. | serum antibody analyses for bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), bovine coronavirus (bcv), and bovine rotavirus (brv) were performed on 527 randomly selected cows, before calving, and on 407 three-week-old calves. in cows and calves, bcv and brv were the most seroprevalent viruses (80% to 100% according to virus and vaccination status). bovine respiratory syncytial virus was the least seroprevalent in the cows, independ ... | 1995 | 7704839 |
| production and characterization of monoclonal antibodies to bovine respiratory syncytial virus. | monoclonal antibodies to the smithkline beecham animal health brsvr vaccine strain (375 isolate of bovine respiratory syncytial virus) were produced and then characterized by radioimmunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, virus neutralization, inhibition of viral-induced fusion and isotype. nineteen hybridomas produced antibodies that were reactive with the f0, f1, n or p viral proteins of bovine respiratory syncytial virus. one hybridoma (1e72c4) ... | 1995 | 7740764 |
| bovine respiratory syncytial virus-specific immune responses in calves after inoculation with commercially available vaccines. | a study was designed to determine if inactivated bovine respiratory syncytial virus (brsv) vaccines induce the same types of antibody and cellular responses as does a modified-live brsv vaccine. ninety mixed-breed, 5- to 6-month-old beef calves were randomly assigned to 1 of 6 groups with 15 animals/group. calves in 5 of the groups were inoculated on days 0 and 14 with 1 of 4 inactivated virus vaccines or with a modified-live virus vaccine. the remaining 15 calves were maintained as unvaccinated ... | 1995 | 7751246 |
| lesions in lambs experimentally infected with bovine respiratory syncytial virus. | an experimental model was designed to characterize lesions in the lung of lambs inoculated with bovine respiratory syncytial virus (brsv). 25 merino lambs of both sexes, with a live weight of 17 +/- 3 kg, received an intratracheal inoculation of 20 ml saline solution containing 1.26 x 10(6) tcid50 brsv (strain nmk-7) per ml. lambs were slaughtered 1, 3, 7, 11 and 15 postinoculation days (pid), and histopathological, immunohistochemical and electron microscopic studies were performed. results ref ... | 1995 | 7756746 |
| antigenic analysis of the f protein of the bovine respiratory syncytial virus: identification of two distinct antigenic sites involved in fusion inhibition. | from two independent fusions, fifteen mabs directed to the f protein of the bovine respiratory syncytial virus (brsv) were characterized by radio-immunoprecipitation assays. competition binding assays among these mabs identified two distinct antigenic sites (a and b) and one overlapping site (ab). all of the mabs specific to epitopes belonging to site a neutralized the infectivity of the virus in vitro and recognized human and bovine rsv strains. only two out of the five mabs directed to epitope ... | 1995 | 7541983 |
| cell-mediated immune responses of lambs to challenge with bovine respiratory syncytial virus. | the lamb is a good model to study the pathogenesis and immune responses to infections with respiratory syncytial virus (rsv) as lambs experimentally infected with bovine or human rsv may develop overt clinical disease. in the present study the development of cellular cytotoxic responses was studied in splenic, pulmonary and peripheral blood mononuclear cells obtained from lambs after primary and secondary infection with bovine rsv. infection with bovine rsv was followed by the appearance of cyto ... | 1995 | 7544251 |
| evaluation and application of an indirect elisa for the detection of antibodies to bovine respiratory syncytial virus in milk, bulk milk, and serum. | an indirect enzyme-linked immunosorbent assay (elisa) was developed at the national veterinary institute (nvi), uppsala, to detect antibodies to bovine respiratory syncytial virus (brsv) in serum and milk. for the evaluation of the nvi elisa, field sera collected from cattle in england and sweden were tested in parallel with an elisa in use at the central veterinary laboratory (cvl), weybridge. the tests showed 96% agreement. the sensitivity and specificity of the nvi elisa relative to the cvl e ... | 1995 | 7619898 |
| development and application of a microneutralization elisa for the detection of antibodies to bovine respiratory syncytial viruses. | a microneutralization enzyme-linked immunosorbent assay (elisa) was developed to detect specific antibodies to bovine respiratory syncytial viruses (brsvs) in cattle sera using a monoclonal antibody to the fusion protein of the virus. serum from 20 naturally exposed, 24 experimentally infected, and 15 immunized cattle were evaluated using 3 different brsv isolates. antibody titers determined with the microneutralization elisa were compared with those derived from a classical virus neutralization ... | 1995 | 7619899 |
| experimental respiratory syncytial virus infection in calves and lambs. | 1995 | 7619920 | |
| role of t-lymphocyte subsets in recovery from respiratory syncytial virus infection in calves. | the role of t-cell subsets in respiratory syncytial virus (rsv) infection was investigated by using monoclonal antibodies (mabs) to selectively deplete gnotobiotic calves of cd4+, cd8+, or wc1+ gamma delta t-cell receptor+ lymphocytes. injection of these mabs produced specific reductions of the target cell populations in the circulation and tissues. ten days after rsv infection, immunoglobulin m (igm), igg1, and iga antibodies were detected in sera and lung washings from control calves. depletio ... | 1995 | 7474075 |
| antibody responses by cattle after vaccination with commercial viral vaccines containing bovine herpesvirus-1, bovine viral diarrhea virus, parainfluenza-3 virus, and bovine respiratory syncytial virus immunogens and subsequent revaccination at day 140. | calves were vaccinated with four different commercial viral vaccines containing bovine herpesvirus-1 (bhv-1), bovine viral diarrhea (bvdv), parainfluenza-3 virus (pi-3v), and bovine respiratory syncytial virus (brsv) immunogens. for the initial vaccination certain vaccines were given twice (days 0 and 28), whereas other vaccines were given on day 0. the calves received another injection on day 140 with the vaccine originally given on day 0. the sera were collected at days 0, 7, 14, 21, 28, 42, 5 ... | 1995 | 7483787 |
| bovine respiratory syncytial virus antibodies in non-bovine species. | to study the role of non-bovine species in the epidemiology of bovine respiratory syncytial virus (rsv) infections, sera obtained from 9 non-bovine animal species and from humans were examined for bovine rsv specific antibodies. sera were mainly from animals and humans which had been in contact with cattle. forty sera of each species were tested in an rsv specific whole virus elisa as well as in a peptide based elisa, that was developed to measure antibodies specific for bovine rsv. antibodies d ... | 1995 | 7487487 |
| bovine respiratory syncytial virus replicates minimally in bovine alveolar macrophages. | the interaction between two different bovine respiratory syncytial virus (brsv) strains and bovine alveolar macrophages (bams) was studied in vitro. bovine respiratory syncytial virus replicated minimally in bams and most of the virus produced remained cell-associated. approximately 1 out of 1,000 bams produced infectious virus, a number that further declined during the 7 days of culture. in contrast, bams exposed to bovine parainfluenza 3 virus (pi3v) produced high amounts of infectious virus. ... | 1995 | 7503690 |
| bovine respiratory syncytial virus reinfections and decreased milk yield in dairy cattle. | the influence of bovine respiratory syncytial virus (brsv) reinfections on the daily milk yield was studied by evaluating the milk production of 32 brsv reinfected cows. for the estimation of milk production losses, four lactation curve models were used, including a gamma function, a second degree polynomial, and both of these models with a lag variable. bovine respiratory syncytial virus reinfections seemed to have only a small effect on the daily milk production. comparison of the true product ... | 1995 | 8525599 |
| t cell epitopes of bovine respiratory syncytial virus attachment glycoprotein. | 1995 | 8566381 | |
| variation in the fusion glycoprotein gene of human respiratory syncytial virus subgroup a. | six different genotypes (designated lineages shl1-6) of human respiratory syncytial (rs) virus have been defined by partial nucleotide sequence analysis of the variable sh and the hypervariable g membrane protein genes, and by restriction fragment analysis of the conserved n protein gene of viruses isolated in south birmingham. viruses of very similar genotype appear to be present worldwide at the present time. we have determined the nucleotide sequences of the fusion protein genes of five virus ... | 1995 | 8808333 |
| a model for respiratory syncytial virus (rsv) infection based on experimental aerosol exposure with bovine rsv in calves. | five conventionally kept calves aged between 17 and 24 days were experimentally infected with bovine respiratory syncytial virus (brsv) by aerosol in order to mimic the natural infection route. the calves were killed and autopsies performed 7 days after the first virus challenge. the brsv isolate used induced tracheitis, bronchitis and atelectasis in infected calves. the only virus which could be isolated from the lungs of the calves was brsv. in addition, mycoplasma bovirhinis was isolated from ... | 1996 | 8814979 |
| immune responses of lambs to the fusion (f) glycoprotein of bovine respiratory syncytial virus expressed on insect cells infected with a recombinant baculovirus. | a group of lambs was immunized with the f protein of bovine respiratory syncytial virus expressed in a baculovirus (bac-f) and their humoral and cellular immune responses to bovine rsv studied before and after challenge with infectious bovine rsv. immunization with bac-f resulted in significant humoral immune responses as measured by virus neutralization and cellular immune responses as measured by lympho-proliferation against inactivated bovine rsv and specific cytotoxicity against autologous t ... | 1996 | 8817824 |
| sites of replication of bovine respiratory syncytial virus in naturally infected calves as determined by in situ hybridization. | replication of bovine respiratory syncytial virus (brsv) was studied in three naturally infected calves by in situ hybridization using strand-specific rna probes. one of the calves was a 5-month-old friesian, the other two calves were a 3-month-old and a 3-week-old jersey. two jersey calves, 3 months and 3 weeks of age, served as controls. replication of brsv took place in the luminal lining of the respiratory tract. in one of the brsv infected animals (calf no. 1), replication was especially se ... | 1996 | 8817835 |
| influence of selective t-lymphocyte depletion on the lung pathology of gnotobiotic calves and the distribution of different t-lymphocyte subsets following challenge with bovine respiratory syncytial virus. | the depletion of cd8+ t-lymphocytes with a murine monoclonal antibody (mab) specific for the cd8 molecule delayed the ability of three gnotobiotic calves to clear bovine respiratory syncytial virus (brsv) from their lungs within 10 days after an experimental challenge with the virus. this protracted infection was associated with an enhanced pneumonic consolidation score (21.6 per cent) compared with seven control calves (7.4 per cent) and a histological lesion of active respiratory epithelial hy ... | 1996 | 8819192 |
| viral aetiology of enzootic pneumonia in danish dairy herds: diagnostic tools and epidemiology. | ten outbreaks of calf respiratory disease in danish dairy herds were investigated by lung lavage, and in eight of the herds paired blood samples were tested serologically. in six of the 10 herds bovine respiratory syncytial virus (brsv) antigen was detected in the lung lavage fluids. in only one calf was coronavirus and brsv detected simultaneously. the paired blood samples confirmed that four of the herds were brsv-infected, and in one herd the brsv infection was diagnosed by the paired blood s ... | 1996 | 8856889 |
| antibody responses against the g and f proteins of bovine respiratory syncytial virus after experimental and natural infections. | antibodies against the two major surface glycoproteins of bovine respiratory syncytial virus (brsv), g and f, play a role in protection against brsv-associated disease, but only the antibody response against the f protein has been well described. therefore, we used a novel peptide-based enzyme-linked immunosorbent assay (g peptide-elisa) to compare immunoglobulin g (igg) and igg subclass antibody responses against the g protein with the antibody response against the f protein, as measured by a c ... | 1996 | 8877125 |
| experimental reproduction of respiratory disease in calves with non-cell-culture-passaged bovine respiratory syncytial virus. | to reproduce experimentally clinical bovine respiratory syncytial virus (brsv) infections in cattle, we isolated brsv from a calf in the field that suffered from acute respiratory disease. cell culture passage of the virus was avoided to prevent any modification of the biological properties of the virus. the isolated brsv was passaged in specific-pathogen-free (spf) calves. lung lavage fluids of these calves, which contained at least 10(3) tcid50/ml brsv and which were found to be free of other ... | 1996 | 8903138 |
| solution structure of the immunodominant region of protein g of bovine respiratory syncytial virus. | the three-dimensional solution structure of the immunodominant central conserved region of the attachment protein g (brsv-g) of bovine respiratory syncytial virus has been determined by nuclear magnetic resonance (nmr) spectroscopy. in the 32-residue peptide studied, 19 residues form a small rigid core composed of two short helices, connected by a type i' turn, and linked by two disulfide bridges. this unique fold is among the smallest stable tertiary structures known and could therefore serve a ... | 1996 | 8942628 |
| morphological studies of the respiratory syncytial virus induced bronchiolitis in experimentally infected calves. | rsv-infections of the lower respiratory tract in infancy and early childhood are the most frequent causes of a hyperreactive bronchial system and obstructive lung disease. studies concerning the morphological alterations of the bronchial mucosa during an rsv-infection are dependent on an experimental animal model. in this study the alterations of the lower respiratory tract from five infected colostrum-fed calves during the initial stage of the infection are described. brsv strain 375 was applie ... | 1996 | 8958555 |
| identification of potential ctl epitopes of bovine rsv using allele-specific peptide motifs from bovine mhc class i molecules. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in young infants and housed calves. depletion of cd8+ lymphocytes from calves inhibited their ability to clear the virus from the nasopharynx and lungs. to study these cells further, a cytotoxic t lymphocyte (ctl) assay was established. ctl could be demonstrated in the peripheral blood of gnotobiotic calves 7-10 days post infection (p.i.) with rsv and in lungs 10 days p.i. this response was both mhc-restricte ... | 1996 | 8988867 |
| subgrouping of bovine respiratory syncytial virus strains detected in lung tissue. | bovine respiratory syncytial virus is an important respiratory pathogen in cattle. recently, subgroups of brsv have been identified, based on antigenic differences. however, little is known about subgroups of brsv that circulate in the cattle population. therefore, we determined the reactivity of monoclonal antibodies (mabs), directed against the g, f, or p protein of brsv, with lung tissue from 47 calves, that suffered from severe respiratory disease. fourteen animals (30%) proved to be infecte ... | 1996 | 9008336 |
| effects of perinatal vaccination on humoral and cellular immune responses in cows and young calves. | to examine the effects of perinatal vaccination on cellular and humoral responses in cows and on passive transfer of antibodies and cells to calves, and to assess the role of maternal antibodies in vaccination responses of neonatal calves. | 1996 | 8575972 |
| mapping the domains on the phosphoprotein of bovine respiratory syncytial virus required for n-p interaction using a two-hybrid system. | specific interactions between the nucleocapsid protein (n) and the phosphoprotein (p) of bovine respiratory syncytial virus (brsv) have been investigated using a yeast-based two-hybrid system. plasmids encoding the yeast gal4 dna binding domain fused with the n gene and gal4 activation domain fused with the p gene were cotransfected into competent yeast cells. the ability of the n and p proteins to interact in vivo was measured by activation of the lacz reporter gene by the gal4 transactivation ... | 1996 | 8609467 |
| fatal pneumonia in adult dairy cattle associated with active infection with bovine respiratory syncytial virus. | 1996 | 8640646 | |
| concern about vaccine study. | 1996 | 8641937 | |
| severe respiratory disease in dairy cows caused by infection with bovine respiratory syncytial virus. | outbreaks of severe respiratory disease caused by bovine respiratory syncytial virus (brsv) were recorded in dairy herds throughout sweden in 1988 and subsequently. the virus was demonstrated in nasopharyngeal swab material from animals in the acute stage of the disease by culture, the polymerase chain reaction (pcr) and by immunofluorescence. serological data from the herds investigated showed that the cows had seroconverted to brsv rather than to bovine coronavirus, bovine viral diarrhoea viru ... | 1996 | 8650902 |
| use of tilmicosin in a severe outbreak of respiratory disease in weaned beef calves. | severe respiratory disease, associated with seroconversion to bovine respiratory syncitial virus (brsv), caused the death of two cattle and necessitated antibiotic treatment of 70 calves (rectal temperature of 39.6 degrees c or greater) from a group of 96 (73%) during an 8-day period. tilmicosin injection resulted in a reduction in median rectal temperature from 40.3 degrees c to 39.2 degrees c and 39.0 degrees c for the first and second days after treatment. the rectal temperature was 39.5 degr ... | 1996 | 8660201 |
| proposed three-dimensional model for the attachment protein g of respiratory syncytial virus. | protein g of respiratory syncytial virus (rsv) is an envelope glycoprotein that is structurally very different from its counterparts (haemagglutinin-neuraminidase and haemagglutinin) in other paramyxoviruses. in this study, we put forward a model for this unique viral envelope protein. we propose that protein g of rsv contains several independently folding regions, with the ectodomain consisting of a conserved central hydrophobic region located between two polymeric mucin-like regions. the centr ... | 1996 | 8683213 |
| type-specific serologic diagnosis of respiratory syncytial virus infection, based on a synthetic peptide of the attachment protein g. | peptides deduced from the central hydrophobic region (residues 158-189) of the g protein of bovine and ovine respiratory syncytial virus (rsv) and of human rsv subtypes a and b were synthesized. these peptides were used to develop elisas to measure specifically antibodies against these types and subtypes of rsv. we have evaluated the bovine rsv-g peptide in both an indirect elisa and in a blocking elisa. specificity and sensitivity, relative to a routine diagnostic elisa that detects antibodies ... | 1996 | 8699029 |
| replication of bovine respiratory syncytial virus in ovine peripheral blood lymphocytes and monocytes in vitro. | adherent and non-adherent mononuclear cells obtained from the peripheral blood of normal lambs supported the replication of bovine respiratory syncytial virus in vitro. sequential treatment of monocytes with phorbol ester acetate (pma) enhanced their ability to support viral replication. after exposure in vitro for 24 h, viral antigens were present in 47 +/- 4.5% of monocytes and 32 +/- 3% of lymphocytes. treatment of monocytes with pma resulted in the increase of the proportion of cells express ... | 1996 | 8701568 |
| production of influenza virus in cell cultures for vaccine preparation. | influenza virus strains of different types for use as an inactivated vaccine have been successfully grown in different cell lines. increasing titres were obtained with bhk-21/brs, vero and mdck cells. cultures in stationary flasks, in spinner cultures or in large bioreactor systems were tested and the optimal conditions were studied. mdck cells grown in serum-free medium before and during the virus production phase were found to yield high titres in the presence of trypsin. satisfactory results ... | 1996 | 8718593 |
| quantitative investigation of population persistence and recurrent outbreaks of bovine respiratory syncytial virus on dairy farms. | to determine if transmission of virus among seropositive cattle is a plausible mechanism for the permanent presence of bovine respiratory syncytial virus (brsv) in dairy herds, and how likely, with the scenario for persistence, there will be only 1 clinical outbreak of brsv per year. | 1996 | 8723872 |
| pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs. | an experimental model was designed to characterize lesions in the lung of lambs inoculated with bovine respiratory syncytial virus (brsv). twenty-five merino lambs of both sexes, with a live weight of 17 +/- 3 kg, received an intratracheal inoculation of 20 ml saline solution containing 1.26 x 10(6) tcid50 brsv (strain nmk-7) per ml. lambs were slaughtered 1, 3, 7, 11 and 15 days postinoculation (dpi), and samples were taken for analysis using light-microscopic and immunohistochemical techniques ... | 1996 | 8767769 |
| primary cytotoxic t-cell responses to bovine respiratory syncytial virus in calves. | bovine respiratory syncytial virus (rsv) is a major cause of respiratory disease in young calves. recent studies in calves, in which different t-cell subsets were depleted, have shown that cd8+ t cells play a central role in recovery from rsv infection. the present study demonstrates that rsv-specific, major histocompatibility complex-restricted cytotoxic t cells appear in the peripheral blood of gnotobiotic calves 7-10 days after infection with bovine rsv and were also detected in the lungs 10 ... | 1996 | 8774360 |
| effect of supplemental chromium on antibody responses of newly arrived feeder calves to vaccines and ovalbumin. | two trials were conducted to investigate the effects of supplemental chromium (cr) from organic sources (cr chelate and high cr yeast) on antibody responses of newly arrived feeder calves following vaccination with infectious bovine rhinotracheitis (ibr), para-influenza-3 (pi3), bovine respiratory syncytial virus (brsv), bovine viral diarrhea (bvd) and pasteurella haemolytica and ovalbumin (ova). using cross bred steer calves purchased at sales in ontario, vaccines and ova were given on d 0 and ... | 1996 | 8785720 |
| serological responses in calves to vaccines against bovine respiratory syncytial, infectious bovine rhinotracheitis, bovine viral diarrhoea and parainfluenza-3 viruses. | the istituto superiore di sanità (iss), the national veterinary services laboratory in italy, is in charge of assessing the quality, safety and efficacy of veterinary vaccines before and after licensing. to evaluate the relative potency of several vaccines against bovine respiratory syncytial virus (brsv), infectious bovine rhinotracheitis virus (ibrv), bovine viral diarrhoea virus (bvdv) and parainfluenza-3 virus (pi3v), the serological responses in vaccinated calves were studied. vaccination w ... | 1996 | 8785944 |
| mapping functional domains in acmnpv pp31. | autographa californica nuclear polyhedrosis virus (acmnpv) replicates in the nucleus and produces a viral-modified form of the nuclear matrix called the virogenic stroma. the virogenic stroma is the site of viral dna packaging and nucleocapsid assembly and is thought to be the site of viral dna replication and rna transcription. acmnpv encodes a phosphoprotein, pp31, which localizes to the nucleus of uninfected insect cells and to the virogenic stroma of infected insect cells. pp31 has dna bindi ... | 1996 | 8806516 |
| cell-mediated immune responses of lambs to experimental infection with bovine respiratory syncytial virus. | primary infection of lambs with bovine respiratory syncytial virus (rsv) was characterised by the presence of virus-specific cytotoxic cells in the peripheral blood as early as 5 days post-infection. these effector cells lysed virus-infected autologous targets in a self-restricted manner. depletion techniques revealed that cytotoxic activity was largely due to ovcd8+ cells. neutrophils obtained from experimentally infected lambs 5 to 10 days post-inoculation exhibited significant cytotoxic activ ... | 1996 | 9157688 |
| antigens of bovine respiratory syncytial virus in peripheral blood lymphocytes of experimentally infected lambs. | eight lambs were experimentally infected with bovine respiratory syncytial virus (rsv) and the distribution of viral antigen in lymphocyte subpopulations studied by flow cytometry. experimental infection with bovine rsv was characterised by significant changes in lymphocyte subpopulations. infection was followed by a significant (p <0.001) reduction in the number of cells expressing the ovcd5 epitope (t cells), due to a fall in the number of cells expressing the ovcd4 epitope (helper) and those ... | 1996 | 9157689 |
| immunological response of mice to the bovine respiratory syncytial virus fusion glycoprotein expressed in recombinant baculovirus infected insect cells. | bovine respiratory syncytial virus (brsv) is a major cause of respiratory disease in calves. the brsv genome encodes two major glycoproteins, g and f, which are the major targets for the host antibody response. we have expressed the f glycoprotein in insect cells (sf9) using a recombinant baculovirus vector. a comparison of the f protein expressed in mammalian and insect cells by sds-page showed that only part of the baculovirus-produced protein was soluble and processed like the native protein. ... | 1996 | 9526539 |
| milk production and reproductive performance in dairy cows given bovine respiratory syncytial virus vaccine prior to parturition. | to assess the effect of vaccination against bovine respiratory syncytial virus on milk production, reproductive performance, and health in lactating dairy cows. | 1997 | 9187730 |
| modulation of bovine t cell responses by il-12 and the influence of il-4 on interferon-gamma responses to respiratory syncytial virus. | 1997 | 9191335 | |
| heparin-dependent attachment of respiratory syncytial virus (rsv) to host cells. | in this study we could demonstrate that heparin (ed50 = 0.32 +/- 0.12 microgram/ml), but not heparan sulphate or chondroitin sulphate c is able to inhibit in vitro infection of cells by respiratory syncytial virus (rsv). in addition, this protective effect of heparin could only be observed, when heparin was present at the time of inoculation. enzymatic digestion of cell surface glycosaminoglycans with heparinase and heparitinase, but not chondroitin sulphate abc lyase reduced the effectiveness o ... | 1997 | 9229012 |
| macrophages and respiratory viruses. | this review highlights some of the interactions between alveolar macrophages and human/swine influenza a viruses, porcine reproductive and respiratory syndrome virus (prrsv), bovine parainfluenza type-3 (pi-3) virus, bovine respiratory syncytial virus (brsv) and bovine viral diarrhoea virus (bvdv). in influenza a infection, macrophage-cytokines are believed to mediate the typical constitutional effects and inflammation in the respiratory tract. the same cytokines possibly play a role in influenz ... | 1997 | 9247041 |
| analysis of bovine respiratory syncytial virus envelope glycoproteins in cell fusion. | to compare the requirements for respiratory syncytial virus (rsv)-mediated cell fusion, the fusion (f), attachment (g) and small hydrophobic (sh) glycoproteins of bovine rsv (brsv), ovine rsv (orsv) and human rsv (hrsv) were expressed individually or coexpressed in either homologous or heterologous combinations in hela cells, using the vaccinia virus-t7 polymerase transient expression system. cell fusion was examined by a reporter gene activation assay. although the expression of the f protein a ... | 1997 | 9266983 |
| immunization with a peptide derived from the g glycoprotein of bovine respiratory syncytial virus (brsv) reduces the incidence of brsv-associated pneumonia in the natural host. | previous reports demonstrate that synthetic peptides corresponding to the amino acid region 174-187 of g glycoprotein from subgroups a and b human respiratory syncytial virus (hrsv), containing a cys-->ser substitution at position 186, confer complete resistance to immunized balb/c mice against infection with the respective virus. in this report, we show that a cys186-->ser substituted peptide (bg/174-187) representing the corresponding region of the bovine (b) rsv g glycoprotein conferred compl ... | 1997 | 9302749 |
| bovine respiratory syncytial virus infection in lactating cows. | 1997 | 9342090 | |
| role of envelope glycoproteins of bovine respiratory syncytial virus in cell fusion. | to investigate the requirements for bovine respiratory syncytial virus (brsv) cell fusion, the fusion (f), the attachment (g) and the small hydrophobic (sh) glycoproteins were expressed individually or coexpressed, using the vaccinia virus-t7 polymerase transient expression system. the contribution of individual glycoproteins in cell fusion was studied by a reporter gene activation assay. activation of a reporter gene, beta-galactosidase, was assessed by colorimetric assay of detergent cell lysa ... | 1997 | 9343948 |
| bovine respiratory syncytial virus. | since the first report of brsv in the 1970s, the understanding of this agent and its respective disease has increased dramatically. current evidence supports a major role for this virus in bovine respiratory disease. advances in diagnostics have increased the ability to demonstrate this virus in field outbreaks of respiratory disease. the clinical signs and pathologic features have been well described, and vaccines are available to aid in prevention and control. still, many questions remain to b ... | 1997 | 9368988 |
| bovine respiratory tract disease caused by bovine viral diarrhea virus. | although several viruses and bacteria are capable of inducing bovine respiratory tract disease, a pivotal organism in the cause of this complex disease may be bovine viral diarrhea virus (bvdv). circumstantial evidence has long supported this hypothesis. it is frequently present in diseased respiratory tract tissues often together with other viruses or bacteria. field observations suggest marked synergism occurs. researchers have confirmed that, in most instances, the virus itself elicits only a ... | 1997 | 9368990 |
| recombinant vaccinia viruses expressing the f, g or n, but not the m2, protein of bovine respiratory syncytial virus (brsv) induce resistance to brsv challenge in the calf and protect against the development of pneumonic lesions. | the immunogenicity and protective efficacy of recombinant vaccinia viruses (rvv) encoding the f, g, n or m2 (22k) proteins of bovine respiratory syncytial virus (brsv) were evaluated in calves, the natural host for brsv. calves were vaccinated either by scarification or intratracheally with rvv and challenged 6 to 7 weeks later with brsv. although replication of rvv expressing the f protein in the respiratory tract was limited after intratracheal vaccination, the levels of serum and pulmonary an ... | 1997 | 9400970 |
| immunization of cattle with a bhv1 vector vaccine or a dna vaccine both coding for the g protein of brsv. | a ge-negative bovine herpesvirus 1 (bhv1) vector vaccine carrying a gene coding for the g protein of bovine respiratory syncytial virus (brsv) (bhv1/brsv-g) induced the same high degree of protection in calves against brsv infection and bhv1 infection as a multivalent commercial vaccine. a dna plasmid vaccine, carrying the same gene as the bhv1/brsv-g vaccine, significantly reduced brsv shedding after brsv infection compared with that in control calves, but less well than the bhv1/brsv-g vaccine ... | 1997 | 9413101 |
| [respiratory syncytial viruses: viral characteristics and epizootiology of the disease]. | human respiratory syncytial virus (hrsv) is the major respiratory tract pathogen of infants and young children. bovine respiratory syncytial virus (brsv) is recognized as an important cause of respiratory tract disease in calves. both these viruses and their respective diseases share many similarities. after a description of the general properties of the virus, this review covers the epizootology of bovine respiratory syncytial virus infection. | 1997 | 9441497 |
| [respiratory syncytial viruses: pathogenesis, immunology and disease prevention]. | immunopathologic mechanism has been proposed to be involved in the pathogenesis of respiratory syncytial virus infections. this review examines the current understanding of the role of immunopathologic mechanisms in rsv infections. the role of vaccines in inducing hypersensitivity is also discussed. | 1997 | 9441498 |
| analysis of the bovine respiratory syncytial virus fusion protein (f) using monoclonal antibodies. | seven monoclonal antibodies (mabs) directed against bovine respiratory syncytial virus (brsv) fusion (f) protein were produced and characterized by radioimmunoprecipitation and immunofluorescence assays. these seven mabs together with the previously described mabs (beeler and van wyke coelingh, 1989) to the f protein of human respiratory syncytial virus (hrsv) were used to study the antigenic variation of 12 strains of ungulate rsv. all except one mabs specific for the hrsv-f protein reacted wit ... | 1997 | 9453129 |
| antibody responses against epitopes on the f protein of bovine respiratory syncytial virus differ in infected or vaccinated cattle. | the fusion protein f of bovine respiratory syncytial virus (brsv) is an important target for humoral and cellular immune responses, and antibodies against the f protein have been associated with protection. however, the f protein can induce antibodies with different biological activity, possibly related to distinct antigenic regions on the protein. therefore, epitopes were mapped on the f protein using monoclonal antibodies. two epitopes (a and b) were identified that induced neutralizing antibo ... | 1997 | 9672586 |
| role of individual n-linked oligosaccharide chains and different regions of bovine respiratory syncytial virus fusion protein in cell surface transport. | the role of n-linked glycosylation and various domains of the fusion (f) protein of bovine respiratory syncytial virus strain a51908 during transport to the cell surface were determined by mutating the four glycosylation sites and by constructing various truncation mutants. the cell surface expression of various mutant proteins was detected by surface immunofluorescence and cell surface biotinylation assay. we found that the glycosylation at positions gly1, gly2, gly3, and a truncation mutant wi ... | 1997 | 9672596 |
| serological indication for persistence of bovine respiratory syncytial virus in cattle and attempts to detect the virus. | to identify putative persistent bovine respiratory syncytial virus (brsv) infections in cattle, seven cattle that had experienced brsv infections were treated with corticosteroids for two periods of 5 days. during the 5-day periods and the 3 weeks after treatment, attempts were made to isolate brsv from lung lavage fluid and nasal swab specimens. fluorescent antibody tests were used to detect brsv antigen in lung lavage cells. a brsv specific polymerase chain reaction (pcr) assay was developed, ... | 1997 | 9672628 |
| functional analysis of antibody responses of feedlot cattle to bovine respiratory syncytial virus following vaccination with mixed vaccines. | the antibody response of cattle to bovine respiratory syncytial virus (brsv) immunization was investigated using 4 different commercially available mixed vaccines. forty, 5-6 month old, beef calves, randomly assigned to groups of 10, were vaccinated on day 0 and 21 with 1 of 3 inactivated vaccines, (3 groups), or a modified live virus (mlv) vaccine. brsv-specific antibody responses were measured prior to vaccination and on day 35 by using an enzyme linked immunosorbent assay (elisa), virus neutr ... | 1997 | 9008797 |