Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| high yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins. | structural proteins from a large-plaque variant (lpv) of human parainfluenza type 3 virus were analysed by electrophoresis on laemmli-type polyacrylamide gels. high virus concentrations were obtained by growth in bs-c-1 cells cultivated on microcarrier beads. purification of the virus in composite equilibrium gradients of potassium tartrate:glycerol resulted in 25% recovery of input infectivity and a preparation containing less than 0.08% of input host cell protein and rna. parainfluenza type 3 ... | 1981 | 6270242 |
| [substances with antiviral activity. xix. amidinohydrazones of formylesters and formylthioesters with aromatic n-heterocyclic substrates]. | some formylthioester and formylester amidinohydrazones with aromatic n-heterocyclic moieties, were synthesized and screened in vitro against the mp mutant of herpes simplex virus type 1 [hsv-1 (mp)] and parainfluenza type 3 virus, ha-1/cr-8 strain. the amidinohydrazone of 1-phenyl-4-formyl-3-(ethylthio)carbonylpyrrole (i) was the most active compound. | 1981 | 6271568 |
| parainfluenza 3 virus: plaque-type variants lacking neuraminidase activity. | virus clones lacking detectable neuraminidase activity (sc-yn and m-yn) as well as those possessing it (lt-910n and lt-yn) were isolated from bovine strains of parainfluenza 3 virus. lt-910n and lt-yn viruses produced large turbid plaques in mdbk cells, and sc-yn virus produced small clear plaques. incorporation of a bacterial neuraminidase in agar overlay medium made sc-yn virus form large turbid plaques, whereas it made m-yn virus form large clear plaques. however, m-yn virus formed only pinho ... | 1981 | 6271683 |
| [growth of bovine fetal cells after intrauterine virus infection of the fetus]. | 1981 | 6272468 | |
| patterns of shedding of myxoviruses and paramyxoviruses in children. | in the houston family study, young children were cultured for virus weekly or biweekly and during acute respiratory illnesses. the interval between the onset of illness and positive culture was examined for 179 infections during 1975-1979. in week 1 after onset, 73%, 73%, and 66% of cultures were positive for influenza a virus, respiratory syncytial virus (rsv), and parainfluenza virus type 3, respectively. pooled data from influenza b virus infections in 1977 and 1980 showed that 73% of culture ... | 1981 | 6273473 |
| [brain lesions in children with para-influenza]. | an analysis of 1274 autopsy protocols of children parainfluenza was revealed as the main disease in 73 and as a concomitant disease in 107 cases, not infrequently as a generalized infection. in 13 observations a detailed study of the brain was performed. in 4 cases the involvement of the brain consisted mainly in cushion-like outgrowth of ependymocytes and endotheliocytes of the blood vessels. in 1 case a comprehensive analysis revealed isolated meningoencephalitis induced by type 3 parainfluenz ... | 1981 | 6274279 |
| causes of atypical pneumonia: results of a 1-year prospective study. | in a protocol study of cases of atypical pneumonia over a 1-year period an etiologic agent was established in 16 cases: legionella pneumophila in 8, coxiella burnetii in 3, chlamydia trachomatis in 2, mycoplasma pneumoniae in 1, para-influenza 3 virus in 1 and cytomegalovirus in 1. in the remaining 11 cases no agent was identified; the illnesses in these cases tended to be less severe. the pneumonia took much longer to resolve in the patients with legionnaires' disease than in all the other pati ... | 1981 | 6275975 |
| virus infection of endothelial cells. | endothelial injury is important in the pathogenesis of thrombosis, atherosclerosis, disseminated intravascular coagulation, and vasculitis. the ability of several common human viruses to infect cultures of endothelial cells obtained from human umbilical veins or bovine thoracic aorta was demonstrated. indicators of infection included cytopathology, viral growth curves, and antigen detection by immunofluorescence. herpes simplex virus type 1, adenovirus type 7, measles virus, and parainfluenza vi ... | 1981 | 6260874 |
| anti-viral cytotoxic lymphocyte response in hamsters with parainfluenza virus type 3 infection. | 1981 | 6261540 | |
| antiviral compounds. xvii - synthesis and in vitro antiviral activity of 1-substituted 2-chloro- and 2-methoxyindole-3-carboxaldehyde thiosemicarbazones. | a series of indole-3-carboxaldehyde thiosemicarbazones with substituent variations at the 1 and 2 positions were prepared and evaluated for in vitro antiviral activity against vaccinia virus strain ihd and parainfluenza virus type 3, ha-i/cr-8 strain. the inhibitory activity of most compounds was restricted to the vaccinia virus, only compounds (iii e) and (iii f) being effective against both viruses. structure-activity relationship are discussed. | 1981 | 6262104 |
| a terminal labelling assay using 3h-uridine to measure cell-mediated cytotoxic responses against parainfluenza type 3 infected target cells. | the standardisation of a terminal labelling cytotoxicity assay which used the incorporation of 3h-uridine to measure target cell survival of the effector phase is described. this assay was applied to a preliminary study of the cell-mediated responses of sheep following intravenous inoculation of pi-3 virus. the assay provided a simple, accurate, and reproducible method to measure cytotoxic responses against pi-3 infected target cells. the experimental data did not require mathematical correction ... | 1981 | 6262347 |
| sensitive plaque neutralization assay for parainfluenza virus types 1, 2, and 3 and respiratory syncytial virus. | a sensitive plaque neutralization assay for parainfluenza virus types 1, 2, and 3 and respiratory syncytial virus was developed in vero and ma 104 cell cultures. the tests were performed in semimicrotoiter trays containing 24 wells, 16 mm in diameter. parainfluenza virus type 1 formed plaques in vero and ma 104 cells only when trypsin was added to the overlay medium. plaquing of parainfluenza virus type 1 was more sensitive and technically reproducible in ma 104 cells than in vero cells. parainf ... | 1981 | 6262372 |
| antibody to parainfluenza virus types 1, 2 and 3 in sera and nasal secretions of persons of different age. | serum and secretory antibody levels against 3 types of parainfluenza virus were determined in persons ranging in age from 1 month to 74 years. with a slight decline in the group of 7 months to 2 years of children, the number of positive findings increased with age, reaching maximum in the groups of 16-24 years [types 1 and 2] and 25-74 years [type 3] old persons. nasal secretory antibodies against parainfluenza type 3 virus were detected in all age categories, the highest number of positive find ... | 1981 | 6262404 |
| giant cell pneumonia associated with parainfluenza virus type 3 infection. | giant cell pneumonia associated with parainfluenza virus type 3 infection and chronic poliovirus type 2 meningoencephalomyelitis are documented in an infant with combined immunologic deficiency (swiss type). caution should be exercised in attributing cases of giant cell pneumonia to measles virus without serologic or virologic evidence. | 1981 | 6265340 |
| [substances with antiviral. xvi. thiosemicarbazones containing benzofuran and 1h-indene moieties]. | a few hydrazones with benzofuran and 1h-indene moieties were synthesized and screened in vitro against vaccinia virus strain ihd, parainfluenza type 3 virus strain ha-1/cr-8, and the mp mutant of herpes simplex virus type 1 [hsv-1 (mp)]. only the thiosemicarbazones were active. the thiosemicarbazone of 3-chloro-2-formyl-1h-indene inhibited vaccinia virus to a greater extent than methisazone, used as reference standard. furthermore, this compound was active also against parainfluenza and herpes v ... | 1981 | 6258974 |
| prevalence of antibodies to parainfluenza-3 virus in various wildlife species and indigenous cattle sharing the same habitats in kenya. | 1981 | 6279891 | |
| [detection of pi-3 viruses, bvd-md viruses and parvoviruses in bovine fetuses after experimental intrauterine infections]. | growing organ cultures prepared from foetuses experimentally infected in utero by the viruses pi-3 and bvd-md or bovine parvovirus proved to be suitable in tests with the re-isolation of these viruses 7, 14, 21, 35, 42 and 70 days after infection. the attempts at demonstrating them by the traditional method of the inoculation of the primary cell culture of foetal kidneys were successful only at the re-isolation of the pi-3 virus seven days after infection. the pi-3 virus without cytopathic effec ... | 1981 | 6277080 |
| [multiplication of the ibr, pi-3 and bvd-md viruses in cell and organ cultures of bovine fetuses after experimental intrauterine infections]. | the intrauterine infection of four- to nine-month-old bovine foetuses with the pi-3, bvd-md viruses, performed 7 to 72 days prior to their delivery, did not exert any significant influence upon the susceptibility of primary cell cultures from foetal organs and tissues to further viral infection in vitro. the bvd-md and ibr viruses multiplied in the primary cell cultures from the organs of a foetus infected with the pi-3 virus seven days before delivery even in the presence of endogenous pi-3 vir ... | 1981 | 6277081 |
| immunity to parainfluenza type 3 virus and pasteurella haemolytica in sheep. | 1981 | 6277166 | |
| susceptibility of cell line cultures of bovine kidney origin to infectious bovine rhinotracheitis and parainfluenza-3 viruses. | 1981 | 6277558 | |
| bioassay system for determining ribavirin levels in human serum and urine. | a simple, sensitive, micromethod was developed to determine levels of ribavirin, or its active metabolic products, in human serum or urine. the procedure utilized the inhibition of measles virus cytopathic effect in bs-c-1 cells. based upon maximum dilutions of human serum or urine containing ribavirin which inhibited the measles virus, the bioassay detected ribavirin in concentrations as low as 0.006 microgram/ml in serum or 0.03 microgram/ml in urine. herpesvirus 1, parainfluenza virus 3 and r ... | 1981 | 7011693 |
| inhibition of respiratory syncytial virus-host cell interactions by mono- and diamidines. | several aromatic mono- and diamidines were found to block cell fusion induced by respiratory syncytial virus. the best inhibitors were able to achieve complete suppression of syncytium formation at a concentration of 1.0 microm. inhibition occurred in respiratory syncytial virus-infected hep-2 and cv-1 cells, but the same inhibitors were ineffective in preventing fusion induced by parainfluenza virus type 3. the fusion inhibitors did not reduce single-cycle virus yields, but did reduce multiple- ... | 1981 | 7247387 |
| persistent parainfluenza virus shedding during isolation at the south pole. | persistent parainfluenza virus shedding in healthy young adults occurred throughout the 8 1/2-month winter isolation period at amundsen-scott south pole station during 1978. two episodes of respiratory illness were observed after 10 and 29 weeks of complete social isolation. throat swabs collected both routinely, and during each outbreak of respiratory illness, were directly inoculated into cell cultures. parainfluenza virus types 1 and 3 were recovered from both symptomatic and asymptomatic sub ... | 1981 | 6256653 |
| parainfluenza 3 infection blocks the ability of a beta adrenergic receptor agonist to inhibit antigen-induced contraction of guinea pig isolated airway smooth muscle. | guinea pigs, actively sensitized to ovalbumin, were inoculated by nasal insufflation with parainfluenza 3 or virus growth medium 4 d before performing in vitro pharmacological studies on tracheal and bronchial smooth muscle. in each airway segment, cumulative dose-response effects of ovalbumin were obtained in the absence and presence of a maximally effective concentration of a beta adrenergic receptor agonist, sulfonterol. sulfonterol shifted the dose-response curve to the right and reduced the ... | 1981 | 6109736 |
| clinical evaluation of radioimmunoassay of nasopharyngeal secretions and serology for diagnosis of viral infections in children hospitalized for respiratory infections. | viral diagnosis was performed using radioimmunoassay (ria) for virus antigen in nasopharyngeal secretions (nps) and complement-fixation (cf) tests of paired sera from specimens of 90 children hospitalized for acute respiratory infection. major respiratory viruses sought for by both methods (adenoviruses, influenza a and b viruses, parainfluenza virus type 3, respiratory syncytial virus) were detected in 40 (44%) of the patients; 15% of the diagnoses were made by nps-ria alone. serologic diagnosi ... | 1982 | 7163028 |
| evidence of a t-cell-mediated cytotoxic response to parainfluenza virus type 3 pneumonia in hamsters. | 1982 | 6278144 | |
| comparative study of vaccines in preventing respiratory disease in beef calves after weaning. | vaccines against infectious bovine rhinotracheitis (ibr), bovine viral diarrhea (bvd), bovine parainfluenza-3 (pi-3), and bacterin against pasteurella hemolytica and p. multocida were studied to determine their effectiveness when given 30 days before weaning in preventing respiratory disease in beef calves after weaning. a total of 310 calves, 6 to 8 months old, were divided into 3 groups. group i consisted of 51 calves vaccinated with a temperature sensitive mutant vaccine intranasally (ibr-pi- ... | 1982 | 6280257 |
| characterization of parainfluenza type 3 virus isolated from the lung of a lamb with pneumonia. | a virus designated dh-1 was isolated from the lung of a lamb that died during the course of an epizootic of acute respiratory tract disease. the virus contained ribonucleic acid and was sensitive to heat (56 c), lipid solvent, and acid (ph 3.0). inoculated cell cultures absorbed and culture fluids agglutinated guinea pig erythrocytes. morphogenic and morphologic characteristics of the isolate were compatible with those of the genus paramyxovirus of the family paramyxoviridae. serologic results i ... | 1982 | 6280532 |
| the pulmonary clearance of pasteurella haemolytica in calves given corynebacterium parvum and infected with parainfluenza-3 virus. | four control calves were aerosolized with parainfluenza-3 and one week later with pasteurella haemolytica. three calves were given corynebacterium parvum at a dose of 15 mg/m2 body surface area, infected with parainfluenza-3 virus one week later, and aerosolized with p. haemolytica two weeks after c. parvum injection. all calves were killed four hours after p. haemolytica exposure and the bacterial retention in the lung was determined. parainfluenza-3 viral infection did not exert any suppressiv ... | 1982 | 6280824 |
| kawasaki syndrome in two cousins with parainfluenza virus infection. | 1982 | 6283878 | |
| natural cell-mediated cytotoxicity of bovine mononuclear cells against virus-infected cells. | the ability of mononuclear cells (mc) from peripheral blood of normal cattle to lyse a variety of cells was tested in a 51cr-release microcytotoxicity assay. several types of bovine cells infected with parainfluenza 3 virus (pi3v) were susceptible to natural cytotoxicity. bovine cells infected with infectious bovine rhinotracheitis virus or noncytopathogenic bovine viral diarrhea virus, uninfected bovine cells, and human cell lines molt-3, hsb-2, k562, and u-937 were not susceptible. the period ... | 1982 | 6284642 |
| natural history of parainfluenza virus infection in childhood. | in order to determine the natural history of parainfluenza virus infection in early life, we followed prospectively 130 infants and children from birth or a few months of age for evidence of infection with pv. using rapid diagnostic techniques, standard tissue culture infectivity, and serologic techniques we were able to document primary pv infection in 92% of these infants, and repeated infection with heterotypic or homotypic pv strains in 49% by 30 months of age. increasing patient age had no ... | 1982 | 6284899 |
| prevalence of antibodies of selected infectious disease agents in the peninsular desert bighorn sheep (ovis canadensis cremnobates) of the santa rosa mountains, california. | 1982 | 6285010 | |
| virologic study of the reproductive tract in dairy cows. | from aug 1, 1979 to aug 15, 1980, a total of 624 samples were collected from the vaginas, cervices, and uteri of 208 dairy cows 30 to 37 days after parturition. an additional 90 samples were collected from the reproductive tracts from 30 of the 208 cows. the 30 cows had aborted or were repeat breeders. samples from cows that had aborted were collected within a week after clinical abortion or immediately after rectal palpation revealed subclinical abortion. all samples were tested for the presenc ... | 1982 | 6285771 |
| prevalence of bovine herpesvirus-1, bovine oral diarrhea, parainfluenza-3, bovine adenoviruses-3 and -7, and goat respiratory syncytial viral antibodies in goats. | sera from healthy goats were collected during october 1979 through october 1980. these sera were tested for bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, bovine adenoviruses (bav) -3 and -7, and goat respiratory syncytial virus (grsv) antibodies by microtitration virus-neutralization test. the number of herds with seropositive goats for each virus were: 5/38 (13.2%) for bhv-1; 9/38 (23.7%) for bvdv; 8/38 (21.1%) for pi-3 virus; 1/38 (2.6%) for ba ... | 1982 | 6285781 |
| lung infiltrates following removal of a neck mass. | 1982 | 6286452 | |
| selection of temperature-sensitive mutants in persistent infection by parainfluenza virus type 3. | vero cells persistently infected with parainfluenza virus type 3 (para 3) were examined for the production of temperature-sensitive (ts) mutants. after 6 months and 33 passages, ts mutants formed the great majority of the virus being shed into the supernatant fluid. complementation studies gave evidence that the mutants shared a common lesion. although ts mutants may play a role in the maintenance of persistent infection in this system, the mutants proved to be unstable when removed from the mil ... | 1982 | 6286859 |
| immune responses of dairy cattle to parainfluenza-3 virus in intranasal infectious bovine rhinotracheitis-parainfluenza-3 virus vaccines. | two hundred and fifty dairy heifers were vaccinated at three to six months of age with an intranasal infectious bovine rhinotracheitis-parainfluenza-3 vaccine. eighteen additional heifers were tested prior to vaccination and again three to four weeks after vaccination. neither cell-mediated nor humoral immunity was significantly raised to parainfluenza-3 virus in either group of cattle. | 1982 | 6290012 |
| respiratory disease in calves produced with aerosols of parainfluenza-3 virus and pasteurella haemolytica. | in four experiments, 22 calves were exposed to aerosols of parainfluenza-3 virus, followed by pasteurella haemolytica at intervals of three to 13 days. the purpose of each experiment was to study viral-bacterial interactions in the respiratory tracts. two experiments, in which the viral aerosols were diluted by the addition of air, produced sporadic temperature elevations while two experiments with undiluted viral aerosols produced consistent temperature elevations. diluted viral aerosols produc ... | 1982 | 6290015 |
| infection of marmosets with parainfluenza virus types 1 and 3. | infection of wild marmosets (saguinus mystax) with strains of parainfluenza virus types 1 and 3 resulted in acute respiratory infection. virus replication in the upper respiratory tract was of a degree similar to that seen in children acutely infected with parainfluenza viruses. serum antibody developed with both virus types; however, local secretory antibody was not detectable. the infection was transmissible to susceptible animals up to 3 days inoculation of the primary animal. | 1982 | 6290387 |
| tick-borne fever and concurrent parainfluenza-3 virus infection in sheep. | 1982 | 6292262 | |
| [adaptation of attenuated strain of p. parainfluenzae-3 in cultured swine kidney cells and analysis of its immunogenicity]. | strain mihailovgrad of p. parainfluenza-3 was successfully adapted to cell cultures of pig kidney at its 15th passage. its testing for innocuity was carried out through the inoculation of test calves with high amounts of the pathogen. no pathologic, temperature, and anaphylactic reactions were established. this confirmed the harmless character of the attenuated strain. comparative testing for immunogenicity was performed with 8-month-old calves. full coincidence with the antibody response was ob ... | 1982 | 6294967 |
| cold adaptation of parainfluenza virus type 3: induction of three phenotypic markers. | in order to attenuate parainfluenza type 3 virus, a wild type strain that was isolated from a child with respiratory disease was adapted to replicate in african green monkey kidney cells at 20 degrees c. replication at 20 degrees c was not a property of the wild type virus. the virus was serially passaged 45 times in the cold, and clones were selected following passage levels 7, 12, 18, and 45. the population of cold-adapted virus was found to be progressively enriched with temperature sensitive ... | 1982 | 6298358 |
| experimentally induced parainfluenza type 3 virus infection in young lambs: pathologic response. | pulmonary changes in five 1-week-old, colostrum-deprived lambs transtracheally inoculated with parainfluenza type 3 virus were studied by immunofluorescent, microscopic, and ultrastructural techniques. the lambs were killed at postinoculation days (pid) 3, 5, and 7. immunofluorescence specific for parainfluenza type 3 virus was first seen in small airways and alveolar epithelium and later in the lumens of airways and alveoli and, to a lesser extent, in the interstitium of the lungs. grossly, the ... | 1982 | 6299138 |
| evidence of interferon production in the hamster lung after primary or secondary exposure to parainfluenza virus type 3. | experimental infection of the hamster respiratory tract with parainfluenza virus type 3 has been used to study the pathogenesis of viral pneumonia and the host response to infection. in this study, hamsters inoculated intranasally with parainfluenza virus type 3 produced local interferon, which was detected in lung washes obtained by in situ lavage. interferon activity was present as early as 2 days after infection, and titers correlated directly with the quantity of virus recovered in lung wash ... | 1982 | 6178328 |
| partial protection of calves against parainfluenza-3 virus infection by nasal-secretion interferon induced by infectious bovine rhinotracheitis virus. | 1982 | 6179445 | |
| experimental parainfluenzavirus infection in mice: fatal illness with atrophy of thymus and spleen in mice caused by a variant of parainfluenza 3 virus. | intracerebral inoculation of the yn strain of parainfluenza 3 virus was found to induce an acute fatal illness characterized by marked thymic and splenic atrophy in newborn mice. previously we showed that the yn strain contains three distinct plaque-type variants, lt, sc, and m. of these, the m-type variant induced this fatal illness, whereas the other two variants induced hydrocephalus. | 1982 | 6276302 |
| viral receptors on isolated murine and human ependymal cells. | viruses that infect ependyma cause ependymitis in humans and hydrocephalus in experimental animals. we report that reovirus type 1 (which induces hydrocephalus in mice) binds to the surface of isolated human and murine ciliated ependymal cells. with the use of recombinant viral clones, the binding property was mapped to the type 1 viral hemagglutinin, which also determines in vivo the affinity of reovirus type 1 for ependyma. mumps virus, measles virus, parainfluenza type 3, and herpes simplex v ... | 1982 | 6276976 |
| comparative studies on haemagglutinating activity and immunogenicity of bovine parainfluenza-3 virus strains. | 1982 | 6320610 | |
| immunization experiments with an inactivated parainfluenza-3 virus. | 1982 | 6320611 | |
| preliminary survey for antibodies against five bovine viruses in cattle in korea. | 1982 | 6302359 | |
| [virological and morphological studies of a model of parainfluenza-3 virus persistence]. | 1982 | 6303062 | |
| persistence of antibodies and anamnestic response in calves vaccinated with inactivated infectious bovine rhinotracheitis virus and parainfluenza-3 virus vaccines. | persistence of antibodies in calves vaccinated with 2 types of inactivated infectious bovine rhinotracheitis (ibr) virus and parainfluenza-3 (pi-3) virus vaccines were determined. calves seronegative for ibr and pi-3 viruses were inoculated with 2 doses of inactivated ibr virus-pi-3 virus vaccines administered 2 weeks apart. blood samples were obtained from the calves for serum at 2 weeks, 6 months, and 1 year after vaccination. the serums were tested by serum-neutralization tests. antibody resp ... | 1983 | 6303996 |
| electron microscopic evidence for parainfluenza type iii/retrovirus phenotypically mixed particles. | sublines of malignant permanent human fibroblast cell lines were shown to be infected by a type-d retrovirus (pmfv) and by a parainfluenza virus type iii in an earlier study (2, 6). careful electron microscopical investigations of these sublines have proved "viral structural elements" of both viruses in the same cell. we obtained electron microscopic evidence--though rarely--for parainfluenza type iii/pmfv mixed-particles. | 1983 | 6305302 |
| protein subunit vaccines of parainfluenza type 3 virus: immunogenic effect in lambs and mice. | protein subunit vaccines were prepared from a mixture of the haemagglutinin (hn) and fusion (f) glycoproteins of parainfluenza type 3 virus (pi-3). the glycoproteins were isolated in three different forms and characterized by their sedimentation coefficients: 30s protein micelles (a complex of several hn and f glycoproteins devoid of detergent and lipid), 18s protein-tx complexes (a complex of several glycoproteins containing the detergent triton x-100), and 4s protein-tx complexes (probably mon ... | 1983 | 6306152 |
| neuraminidase activity and syncytial formation in variants of parainfluenza 3 virus. | by a sensitive fluorometric assay method, we could definitely demonstrate neuraminidase activity for two variants of parainfluenza 3 virus, m and sc, which were previously shown to have no detectable neuraminidase activity. the enzyme activities of these viruses were very similar to each other, showing a much lower catalytic rate, a much higher km value, and a more acidic ph optimum than those of the virus variants of high neuraminidase activity, 910n, lt, and mr. m and sc viruses eluted from gu ... | 1983 | 6307881 |
| serology of bovine parainfluenza virus type 3: comparison of the enzyme linked immunosorbent assay and hemagglutination inhibition. | an enzyme linked immunosorbent assay (elisa) for the detection of antibody to bovine parainfluenza virus type 3 has been compared with the hemagglutination inhibition test on 130 field sera, and seven other paired sera showing a significant raise of titers. the elisa was found to be four to 64 times more sensitive than the hemagglutination inhibition test and the two tests demonstrated a good correlation. | 1983 | 6309348 |
| ultrastructural features of experimental parainfluenza type 3 virus pneumonia in calves. | ultrastructural changes observed in the acute and repair stages of experimental p13 virus pneumonia in young calves are described. in the early acute stage of the pneumonia, paramyxovirus replication was observed within epithelial cells of the respiratory tract and within alveolar macrophages. there was loss of cilia and of ciliated cells in small bronchi and bronchioli. proliferation of non-ciliated bronchiolar cells (clara cells) produced zones of hyperplastic bronchiolar epithelium and, withi ... | 1983 | 6309920 |
| reactivation of bovid herpesvirus 1 and 2 and parainfluenza-3 virus in calves latently infected. | two experiments were carried out to determine whether bovid herpesvirus (bhv) 2 is able to induce a recurrent infection in experimentally infected calves. in the first experiment the stress induced by dexamethasone (dms) treatment failed to reactivate the clinical condition or to induce shedding of bhv2. however, treatment with dms reactivated a latent bhv1 infection in all calves previously inoculated with bhv2 and also in two noninoculated controls. probably, because of the interference by bhv ... | 1983 | 6313287 |
| disseminated parainfluenza infection in a child with severe combined immunodeficiency. | an 8-month-old male infant with severe combined immunodeficiency syndrome was admitted to the hospital with pneumonitis and, subsequently, died at 21 months of age. during his lengthy hospitalization, parainfluenza virus type 3 (piv-3) was isolated from respiratory secretions and unusual sites, such as pericardial fluid, csf, and wbcs. a postmortem examination showed apparent viral pancreatitis, and piv-3 was isolated from the lung, brain, and pericardial fluid. to our knowledge, this is the fir ... | 1983 | 6314807 |
| effects of bovine parainfluenza-3 virus on phagocytosis and phagosome-lysosome fusion of cultured bovine alveolar macrophages. | bovine parainfluenza-3 virus (pi-3v) replicated in cultured bovine alveolar macrophages (bam) in vitro. cytopathic changes included spindle cell and giant cell formation, diffuse cell lysis, and cellular detachment progressing to total destruction of the monolayer. direct immunofluorescent microscopy revealed viral antigen in greater than 90% of bam on post-inoculation day (pid) 3. virus titers in culture supernatants increased by a factor of 1.6 x 10(6) tcid50 by pid 6. candida glabrata was pha ... | 1983 | 6314852 |
| characteristics of the polyriboinosinic acid:polyribocytidylic acid assay for noncytopathogenic bovine viral diarrhea virus. | the use of polyriboinosinic acid:polyribocytidylic acid (poly i:c) for noncytopathogenic bovine viral diarrhea virus (nc-bvdv) assay (pinba) was studied. several viruses were tested for their suitability as test challenge viruses. in addition to vesicular stomatitis virus, which previously was shown to be a suitable challenge virus, bovine enteroviruses also were found to be suitable, whereas infectious bovine rhinotracheitis virus and parainfluenza type 3 virus were only marginally suitable. bo ... | 1983 | 6314854 |
| a field trial, of preshipment vaccination, with intranasal infectious bovine rhinotracheitis-parainfluenza-3 vaccines. | a total of 849 calves, 278 controls, 335 vaccinated intranasally with ibr-pi3/ts and 236 vaccinated intranasally with ibr-pi3/ptc were studied in a field trial of preimmunization. all calves were vaccinated in saskatchewan at least three weeks prior to shipment to feedlots. four hundred and twenty six calves were not sold within eight weeks of vaccination; however, seven of these died within four weeks of vaccination. treatment rates varied from 1.0% to 5.2%. there was no significant effect of v ... | 1983 | 6315194 |
| the relationship of serology and nasal microbiology to pulmonary lesions in feedlot cattle. | a group of 62 beef calves, born and raised in an institutional herd, were transferred at nine months of age to a commercial feedlot where they remained until slaughter seven months later. clinical, immunological and microbiological monitoring was carried out during this period. no serious clinical illness occurred. one hundred percent seroconversion to bovine virus diarrhea virus took place after introduction of the calves into the feedlot as well as almost complete (59/62) seroconversion to bov ... | 1983 | 6315201 |
| epizootic of parainfluenza-3 virus infection in gibbons. | a laboratory-housed breeding colony of white-handed gibbons (hylobates lar) experienced an epizootic of upper respiratory tract disease characterized by lethargy, anorexia, coughing, and serous rhinorrhea. signs were more severe in adults than in offspring, and all animals recovered without complications. base-line, acute, and convalescent sera from the most severely affected gibbons were tested for antibodies against a wide spectrum of infectious agents. for personnel known to have had contact ... | 1983 | 6315655 |
| prevalence of bovine herpesvirus-1, parainfluenza-3, bovine rotavirus, bovine viral diarrhea, bovine adenovirus-7, bovine leukemia virus and bluetongue virus antibodies in cattle in mexico. | 1983 | 6316006 | |
| antibodies against viral pathogens in dairy cattle in colombia. | 1983 | 6316593 | |
| experimental parainfluenza type 3 infection in young lambs: clinical, microbiological, and serological response. | five, 1-week-old, colostrum-deprived lambs were inoculated transtracheally with a parainfluenza type-3 (pi-3) virus that had been isolated from a pneumonic lamb lung. a biphasic febrile response, cough, rapid breathing followed by forced expirations, listlessness, and anorexia were observed in the lambs. there were multifocal areas of consolidation in the lungs of all lambs and ulcerations in the nasal mucosa of three lambs. serum antibody titers to pi-3 virus ranged from 2 to 16 in lambs necrop ... | 1983 | 6316628 |
| evaluation of the attenuated vaccine para-ribovac. | 1983 | 6316694 | |
| improvement of respiratory syncytial virus replication in actively growing hep-2 cells. | the growth of respiratory syncytial (rs) and parainfluenza type iii (pi3) viruses has been studied in actively growing versus relatively stationary hep-2 cells. there was no effect on pi3 virus growth. rs virus synthesis was stimulated from 20- to 60-fold by growth in actively growing cultures when the cell density was approximately 1/3 that of a confluent culture. this stimulation was manifested by a greater yield of virus per cell, more virus-specific mrna produced per cell, and a 50% decrease ... | 1983 | 6317708 |
| immunosuppression in goats inoculated with parainfluenza type 3 virus. | the humoral and cellular immune responses of goats experimentally infected with an ovine isolate of parainfluenza type 3 virus (pi-3) were examined. virus neutralization and enzyme-linked immunosorbent assays were used to determine antibody in the serum and csf. lymphocyte stimulation, measured by [3h]thymidine incorporation into peripheral blood leukocytes, was used to determine cellular responses to phytomitogens and virus. there were significant suppression of peripheral blood leukocyte respo ... | 1983 | 6318612 |
| variation of virus replication in primary bovine kidney cell cultures derived from 68 three-day-old calves. | primary kidney cell cultures were prepared from 68 three-day-old calves. complete monolayers of these cultures were infected separately with viral diarrhea, infectious bovine rhinotracheitis, parainfluenza, and adenovirus 7 viruses. the yield of virus from all infected cultures was calculated by plaque titer assay after 2 to 4 days' incubation. the variation of virus yield was substantial between individual cultures. | 1983 | 6318615 |
| exposure of calves to aerosols of parainfluenza-3 virus and pasteurella haemolytica. | the present study was undertaken to investigate whether sequential exposure to aerosols of parainfluenza-3 virus followed by pasteurella haemolytica, or p. haemolytica followed by parainfluenza-3 virus, could lead to the production of pulmonary lesions in conventionally-raised calves. twenty male calves with low serum antibody titres to both organisms were placed in five equal groups. synergism of parainfluenza-3 virus and p. haemolytica was not demonstrated in any of the sequentially infected g ... | 1983 | 6320999 |
| inhibition by vasoactive intestinal peptide of antigen-induced histamine release from guinea-pig minced lung. | vasoactive intestinal peptide (vip) was found to inhibit ovalbumin-induced histamine release from guinea-pig minced lung. maximum inhibition occurred with a 10-20 min time of preincubation with vip. spontaneous histamine release was not altered by vip. with 2 x 10(-6) m vip, the dose-response curve to ovalbumin was shifted about 3-fold to the right, but the maximum histamine release was unaltered. respiratory tract infection with parainfluenza 3 virus did not influence the inhibitory effect of v ... | 1983 | 6189733 |
| monoclonal antibodies to epitopes shared by actin and vimentin obtained by paramyxovirus immunization. | three hybridoma clones producing igm antibodies against actin were obtained from mice immunized with purified virions of paramyxoviruses. when tested on growing lung fibroblasts, ascites fluids of all clones stained in immunofluorescence cytoplasmic bundles of microfilaments, but also fibrillar networks. on colchicine-treated cells, perinuclear coils were seen in addition to microfilament bundles. in addition, one clone gave a pronounced speckled staining to the nuclei. absorption of the ascites ... | 1983 | 6190666 |
| lack of virus-specific bacterial adherence to bovine embryonic lung cells infected with bovine parainfluenza virus type 3. | infection of bovine embryonic lung cells with bovine parainfluenza virus type 3 did not induce in vitro, virus-specific, hemadsorption-related adherence of corynebacterium pyogenes, haemophilus somnus, staphylococcus aureus, streptococcus zooepidemicus, pasteurella haemolytica, listeria monocytogenes, escherichia coli, pasteurella multocida, brucella sp., or salmonella typhimurium. | 1983 | 6302133 |
| broad-spectrum antiviral activity of 2-beta-d-ribofuranosylselenazole-4-carboxamide, a new antiviral agent. | the relative in vitro antiviral activities of three related nucleoside carboxamides, ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), tiazofurin (2-beta-d-ribofuranosylthiazole-4-carboxamide), and selenazole (2-beta-d-ribofuranosylselenazole-4-carboxamide), were studied against selected dna and rna viruses. although the activity of selenazole against different viruses varied, it was significantly more potent than ribavirin and tiazofurin against all tested representatives of the ... | 1983 | 6615611 |
| ribavirin treatment of viral pneumonitis in severe combined immunodeficiency disease. | 1983 | 6136856 | |
| the prevalence and microbiology of pneumonia in a flock of lambs. | a flock of new zealand romney lambs on a property in hawkes bay was examined from august 1978 to june 1979. in november, the lambs in the flock were allocated to groups as follows: 600 lambs from which groups were selected for slaughter by commercial criteria each month (selected groups); 700 lambs which were subdivided into 8 groups (random groups), one of which was sent for slaughter each month; and 25 lambs to monitor serological responses to viral infections (surveillance group). at slaughte ... | 1983 | 16030927 |
| vaccination against ovine pneumonia: a progress report. | the results of three field trials of a live, cold-adapted, parainfluenza virus type 3 vaccine supported the hypothesis that infection with this virus initiates some ovine pneumonias. the serological response following vaccination was poor and only 22-44% of vaccinates developed a serum antibody response by two weeks after vaccination. nevertheless, the prevalence of pneumonia was lower in vaccinated lambs in all three trials at the time that natural infection with pi3 was detected in the non-vac ... | 1983 | 16030967 |
| synthesis and antiviral/antitumor activities of certain pyrazolo[3,4-d]pyrimidine-4(5h)-selone nucleosides and related compounds. | several pyrazolo[3,4-d]pyrimidine-4(5h)-selone ribonucleosides were prepared as potential antiparasitic agents. treatment of 4-chloro-1-(2,3,5-tri-o-acetyl-beta-d-ribofuranosyl)pyrazolo [3,4-d]pyrimidine (5a) with selenourea and subsequent deacetylation gave 1-beta-d-ribofuranosylpyrazolo[3,4-d] pyrimidine-4(5h)-selone (6a). a similar treatment of 3-bromo-4-chloro-1-(2,3,5-tri-o-benzoyl-beta-d-ribofuranosyl)pyrazolo [3,4-d]pyrimidine (5b) with selenurea, followed by debenzoylation, gave the 3-br ... | 1984 | 6086922 |
| isolation of avian paramyxovirus 2 and 3 from turkeys in brittany. | 1984 | 6087542 | |
| resolution of two surface glycoproteins from human parainfluenza-3 virus by crossed immunoelectrophoresis. | the technique of two-dimensional crossed immunoelectrophoresis (cie) was used to resolve two glycoproteins from purified human parainfluenza type 3 virus. virus preparations were extracted with triton x-100 and fractionated by centrifugation in a beckman airfuge. two immunoprecipitates were detected by cie in the supernatant fractions, but were not found in the pellets from extracted virus. viral glycoproteins labeled with [35s]methionine were isolated by affinity chromatography on concanavalin ... | 1984 | 6088566 |
| effect of anti-prostaglandin therapy in experimental parainfluenza type 3 pneumonia in weaned, conventional calves. | an acute pneumonia was induced experimentally in 10, 10- to 12-week-old conventional calves by administration into the upper airways of a pathogenic strain of parainfluenza type 3 (pi3) virus. the experimental calves had been selected on the basis of freedom from clinical evidence of respiratory and other diseases, freedom from current infection by pi3 virus as judged by repeated nasopharyngeal swabbing and freedom from earlier pi3 virus infection as judged by their lack of significant levels of ... | 1984 | 6089403 |
| prevalence of antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, bovine herpesvirus-1, and bovine parainfluenza-3 virus in sheep and goats in quebec. | serum samples were collected from 1,075 clinically normal sheep and goats from 77 flocks in 7 agricultural regions of quebec from june to august 1982. sheep and goats were tested for antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, and bovine herpes-virus-1 by the indirect fluorescent antibody technique and for parainfluenza-3 virus by the hemagglutination inhibition test. the prevalence of antibodies in animals to respiratory syncytial virus was 31%; to bovine vira ... | 1984 | 6089626 |
| serological diagnosis of parainfluenza virus infections by enzyme immunoassay with special emphasis on purity of viral antigens. | enzyme immunoassay (eia) for parainfluenza virus type 1, 2, and 3 antibodies was compared with the complement fixation test (cf) as a diagnostic method in 180 patients with respiratory symptoms. the cf test detected rises in parainfluenza virus antibodies in 30 cases, whereas eia detected 47 rises. patients with antibody rises in parainfluenza or mumps virus antibodies were studied for cross-reactions by cf, hemagglutination inhibition (hai), and eia using purified viral envelope glycoprotein an ... | 1984 | 6092530 |
| synthesis and antiviral/antitumor activities of certain 3-deazaguanine nucleosides and nucleotides. | a new procedure for the preparation of the antiviral and antitumor agent 3-deazaguanine (1) and its metabolite 3-deazaguanosine (2) has been developed by reacting methyl 5(4)-(cyanomethyl) imidazole-4(5)-carboxylate (4) and 5-(cyanomethyl)-1- (2,3,5-tri-o-benzoyl-beta-d-ribofuranosyl)imidazole-4-carboxylate (6), respectively, with hydrazine. the 3-deazaguanosine 3',5'-cyclic phosphate (13) was prepared from 5-(cyanomethyl)-1-beta-d-ribofuranosyl-imidazole-4-carboxamide 5'-phosphate. glycosylatio ... | 1984 | 6092634 |
| structural characterization of virion proteins and genomic rna of human parainfluenza virus 3. | the virion proteins and genomic rna of human parainfluenza virus 3 have been characterized. the virion contains seven major and two minor proteins. three proteins of 195 x 10(3) molecular weight (195k), 87k, and 67k are associated with the nucleocapsid of the virion and have been designated l, p, and np, respectively. three proteins can be labeled with [14c]glucosamine and have molecular weights of 69k, 60k, and 46k. we have designated these proteins as hn, f0, and f1, respectively. hn protein h ... | 1984 | 6092708 |
| acquisition of serum antibodies to specific viral glycoproteins of parainfluenza virus 3 in children. | a radioimmunoprecipitation assay was used to study antibody responses to parainfluenza virus 3 glycoproteins in human sera. the method was not only more sensitive than the neutralization test for the detection of antibody but also provided semiquantitative assessments of the antibody response to both glycoproteins in a single assay system. anti-hemagglutinin-neuraminidase titers were consistently higher than anti-fusion levels in the same serum specimen. thirteen children were monitored serologi ... | 1984 | 6092715 |
| parainfluenza virus type 3: seasonality and risk of infection and reinfection in young children. | in houston the temporal occurrence of infections with parainfluenza virus type 3 has evolved from an endemic to an epidemic pattern. continuous virological surveillance for six years demonstrated that most infections occurred the late winter or spring after influenza virus activity. at least two-thirds of children observed in the houston family study were infected with this virus in each of the first two years of life, and the risk of illness was about 30/100 children per year. after two years o ... | 1984 | 6094674 |
| survey for antibodies to respiratory viruses in two groups of sheep in northern ireland. | two hundred serum samples from texel and texel crossbred sheep (non-indigenous breeds) and 200 from indigenous northern ireland breeds (mainly blackface, cheviot and border leicester crosses) were tested for antibodies to parainfluenza virus types 1, 2 and 3, respiratory syncytial virus, bovine adenovirus (subgroups 1 and 2), influenza type a, maedi-visna virus and bovine virus diarrhoea virus. the percentage of animals with antibodies to parainfluenza virus 3 (50 to 56 per cent) and adenovirus ... | 1984 | 6095511 |
| serological studies of parainfluenza type 3 virus, bovine adenovirus type 3 and bovine respiratory syncytial virus infection in beef calves. | six serum samples were taken at monthly intervals from birth to weaning from each of 41 newborn calves in the autumn and spring calf crops of a beef cow--calf herd. the serum hemagglutination-inhibition (hi) antibody titres to parainfluenza type 3 virus (piv-3), virus-neutralization (vn) antibody titres to bovine adenovirus type 3 (bav-3) and bovine respiratory syncytial virus (brsv) were determined using microtitration techniques. there was serological evidence of a significantly higher inciden ... | 1984 | 6095517 |
| predisposition to invasive pneumococcal illness following parainfluenza type 3 virus infection in chimpanzees. | an outbreak of invasive disease, including pneumococcal bacteremia, meningitis, and pneumonia, involved 17 of 83 (20.5%) chimpanzees at a primate rehabilitation unit. invasive disease was more common in splenectomized than in nonsplenectomized animals (42.9% vs 18.4%), but the difference was not statistically significant. the outbreak followed closely an outbreak of upper respiratory tract infection (urti) that occurred with equal frequency in splenectomized and nonsplenectomized chimpanzees. th ... | 1984 | 6096328 |
| prevention of bovine respiratory syncytial virus infection and clinical disease by vaccination. | a double blind field trial was carried out with a live attenuated bovine respiratory syncytial virus vaccine. the trial involved 530 calves, two to 10 months old, on 27 dairy farms, where respiratory problems due to bovine respiratory syncytial virus infections had been observed during the preceding year. in 17 herds either all calves were vaccinated (nine groups) or all calves received a placebo (eight groups). in 10 herds half the number of calves were vaccinated and the other half kept as non ... | 1984 | 6097014 |
| susceptibility of specific pathogen-free lambs to concentrations of pasteurella haemolytica serotype a2 in aerosols. | a 100-fold reduction in the numbers of organisms in an aerosol of pasteurella haemolytica used to infect specific pathogen-free lambs did not alter the number of cases of pneumonia which resulted. in a separate experiment a further 10-fold reduction in the number of organisms in the aerosol did not cause fewer cases of pneumonia. | 1984 | 6097970 |
| [pulmonary infections in pediatrics]. | 1984 | 6097981 | |
| [nosocomial epidemic episode of acute respiratory disease due to para-influenza virus type 3]. | 1984 | 6100623 | |
| antiviral activity of c-5 substituted tubercidin analogues. | the pyrrolo[2,3-d]pyrimidine nucleoside antibiotics tubercidin, toyocamycin, and sangivamycin and the synthetic analogues 5-chloro-, 5,6-dichloro-, 5-bromo-, 6-bromo-, 5,6-dibromo-, 5-iodo-, 5-(1-hydroxyethyl)-, 5-(1-methoxyethyl)-, (e)-5-(2-bromoethenyl)-, (e)-5-(2-cyanoethenyl)-, 5-(2-buten-1-yl)-, 5-(3-hydroxypropyl)-, and 5-butyltubercidin were evaluated for their antiviral properties against six rna viruses and three dna viruses in hela cell, primary rabbit kidney cell, and vero cell cultur ... | 1984 | 6699874 |
| antiviral effects of bovine interferons on bovine respiratory tract viruses. | the antiviral effects of bovine interferons on the replication of bovine respiratory tract viruses were studied. bovine turbinate monolayer cultures were treated with bovine interferons and challenged with several bovine herpesvirus 1 strains, bovine viral diarrhea virus, parainfluenza type 3 virus, goat respiratory syncytial virus, bovine respiratory syncytial virus, bovine adenovirus type 7, or vesicular stomatitis virus. treatment with bovine interferons reduced viral yield for each of these ... | 1984 | 6201505 |
| a glucose oxidase immunoenzyme stain for the detection of viral antigen or antibody on nitrocellulose transfer blots. | separation of mixtures of proteins by polyacrylamide gel electrophoresis followed by transfer of the proteins to support media such as nitrocellulose and detection by immunologic procedures provides a powerful analytic tool for assaying the antibody specificity of antisera or for following the purification of antigens. this technique requires fewer assumptions about antigenic solubility or antibody reactivity than immunoprecipitation methods. we present a glucose oxidase immunoenzyme staining pr ... | 1984 | 6205093 |