Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| demonstration of bovine virus diarrhoea virus antigen in formalin fixed, paraffin embedded tissue using a streptavidin/biotin technique. | the detection of bovine virus diarrhoea virus (bvdv) antigen in sections from formalin fixed, paraffin embedded tissue is described. pre-digestion of the sections with 0.02 per cent protease xiv for 18 hours at 4 degrees c is necessary to unmask formalin fixed antigen. a hyperimmune antiserum prepared in a pig, using a combination of bvdv and hog cholera virus inoculations, linked to a biotinylated anti-pig/streptavidin peroxidase detection system demonstrated antigen in a wide range of tissues ... | 1989 | 2544973 |
| hog cholera virus--characterization of specific antiserum and identification of cdna clones. | a specific antiserum was raised against the pestivirus inducing hog cholera (hog cholera virus, hcv). using immunoprecipitation and sds-page, this antiserum served for comparison of hcv-induced proteins with those from a related and better characterized pestivirus, bovine viral diarrhea virus (bvdv). in addition to immunological relationships, the apparent molecular weights of some proteins induced by both viruses were quite similar. hcv genomic rna was found to be about 12 kb in length, compara ... | 1989 | 2545029 |
| evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to bovine virus diarrhoea virus in milk. | the present study shows that milk is an appropriate source for detection of seroreactors to bovine virus diarrhoea virus (bvdv). there was close agreement between antibody titres in serum and in skim milk, as determined by an indirect enzyme-linked immunosorbent assay. the antibody titres were usually lower in skim milk than in serum, but all seropositive cows (n = 84) were also skim milk-positive and all but one seronegative cow (n = 55) proved negative in skim milk. during lactation, the level ... | 1989 | 2546339 |
| development of monoclonal neutralizing antibodies against bovine viral diarrhea virus after pretreatment of mice with normal bovine cells and cyclophosphamide. | after immunization of mice with bovine viral diarrhea virus (bvdv) concentrated by ultracentrifugation only antibodies against normal cellular constituents were detectable. monoclonal and polyclonal anti-bvdv antibodies were obtained, however, after induction of tolerance against normal bovine cellular antigens by pretreatment of adult mice with bovine cells and cyclophosphamide followed by immunization with bvdv containing material. | 1989 | 2547817 |
| detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. | we propose through a sequence and structural-pattern analysis that a protein domain of undefined function encoded by the enveloped rna flavi- and pestivruses is a ser active-center enzyme related to the cellular trypsin family. a further homology is emphasized with the group of (cys active-center) viral proteases encoded by nonenveloped rna viruses of the picorna-, como-, nepo-, and potyvirus classes. structural modeling of the putative flaviviral protease domain suggests amino acids that are cr ... | 1989 | 2548336 |
| specificity of neutralizing and precipitating antibodies induced in healthy calves by monovalent modified-live bovine viral diarrhea virus vaccines. | serum was obtained at weekly intervals after vaccination of 6 healthy calves with either of 2 commercially available monovalent modified-live bovine viral diarrhea (bvd) virus vaccines. detectable neutralizing antibodies to each of 10 cytopathic and 10 noncytopathic isolates of bvd virus were produced by 1 or more of the calves by 14 days after vaccination, but no calf produced detectable neutralizing antibodies to all 20 bvd viruses. at that time, precipitating antibodies against viral-induced ... | 1989 | 2548419 |
| comparative analysis of monoclonal antibodies against pestiviruses: report of an international workshop. | thirty-three pestivirus strains were grown in cell culture and characterized by immunostaining with 19 monoclonal antibodies (mabs) raised against hog cholera virus (hcv), with 42 mabs against bovine viral diarrhoea virus (bvdv) and with 13 mabs against border disease virus (bdv). seven mabs reacted with all pestivirus strains tested, eight mabs detected only the seven hcv strains, three detected only the 16 bvdv strains. no mab was found that was specific for bdv. bvdv and bdv strains were broa ... | 1989 | 2549680 |
| development of a neutralising antibody response to an inoculated cytopathic strain of bovine virus diarrhoea virus. | fourteen clinically healthy cattle that were persistently infected with non-cytopathic bovine virus diarrhoea virus (bvdv) and three bvdv-free cattle were inoculated with one of three cytopathic bvdv strains. mucosal disease developed in 12 of the viraemic cattle, resulting in a moribund condition 17 to 99 days after inoculation. two of the viraemic cattle remained clinically healthy until the end of the experiment, 14 months after inoculation. the bvdv-free cattle did not develop clinical signs ... | 1989 | 2552639 |
| characterization of porcine and some ruminant pestiviruses by cross-neutralization. | serologic relationships between 11 pestivirus strains that originated from pigs and five that originated from cattle or sheep were studied by cross-neutralization. experiments were performed with pig and sheep sera raised against the strains. the results were analysed by a computerized taxonomic procedure. the 16 viruses were classified into four distinct serologic groups. all hog cholera virus (hcv) strains were classified in one group; the other three groups consisted of strains that can infec ... | 1989 | 2552645 |
| demonstration of bovine viral diarrhoea virus antigens in cell cultures and in paraffin-embedded tissue sections by the peroxidase-antiperoxidase (pap) technique using monoclonal antibodies. | 1989 | 2556905 | |
| detergent solubilised bovine viral diarrhea virus elicits a similar immune response as the inactivated virus. | the immune response to two detergent solubilised preparations of bovine viral diarrhea virus (bvdv) proteins were compared with the response to a chemically inactivated bvdv preparation. the antibody titres were measured with the standard immunofluorescence assay and with a newly developed enzyme linked immunosorbent assay (elisa). the range of viral protein specificity of the antibodies was compared using sds-polyacrylamide gel electrophoresis (sds-page) followed by western blot analysis. the r ... | 1989 | 2558836 |
| a perspective on the pestiviruses. | 1989 | 2559695 | |
| antigenic differentiation of pestivirus strains with monoclonal antibodies against hog cholera virus. | thirty-one bovine viral diarrhoea virus (bvdv) or border disease virus (bdv) strains and 94 hog cholera virus (hcv) strains were grown in cell culture, and characterized by immunostaining with 13 monoclonal antibodies (mabs) and one polyclonal serum (pab) against hcv. all 125 strains were recognized by the pab. none of the bvdv or bdv strains were detected by the 13 mabs. seven mabs detected all 94 hcv strains. six other mabs detected heterogeneity among and within hcv strains. the mabs are usef ... | 1989 | 2560281 |
| [research on antibodies against bhv-1, bhv-2, bhv-4, bvd-md virus, bovine adenovirus a and b, rotavirus and coronavirus in cattle in western zaire: complementary results]. | two-hundred bovine sera from western zaire were screened for antibodies to 8 viruses: bhv-1, bhv-2, bhv-4, bvd-md virus, bovine adenovirus a and b, bovine rotavirus and bovine coronavirus. positive sera were found to all these viruses. for animals whose origin was undoubted, the main features were the high prevalence of infections by rotavirus and bhv-4 and the low prevalence of infections by coronavirus and bvd-md virus. | 1989 | 2560579 |
| bovine pestivirus and human infection. | 1989 | 2562871 | |
| infantile gastroenteritis associated with excretion of pestivirus antigens. | faeces from children under 2 years old who had gastroenteritis that could not be attributed to recognised enteric pathogens were examined with a monoclonal-antibody-based immunoassay for pestivirus antigens. such antigens were detected in 30 of 128 episodes of gastroenteritis. children without diarrhoeal disease and children infected with rotaviruses had little evidence of pestivirus infection (faeces positive in 1 of 28 and 1 of 31, respectively). the diarrhoeal disease in children excreting pe ... | 1989 | 2564059 |
| peroxidase-labeled primary antibody method for detection of pestivirus contamination in cell cultures. | contaminating bovine viral diarrhea (bvd) virus in cell cultures and in fetal bovine serum (fbs) is a well recognized problem. this study describes a direct peroxidase (dp) labeled primary antibody method for detection of pestivirus antigens in cell culture that is simple and reliable. using this method, most bovine and porcine cell cultures, a cat lung, four mosquito and two monkey cell cultures were found contaminated with bvd virus. the rodent and human cell cultures tested were negative by t ... | 1989 | 2574184 |
| an outbreak of mucosal disease in a dairy herd. | an outbreak of mucosal disease (md) was studied in a dairy herd, comprising 12 cows, 9 heifers and 18 calves. during a period of 1 month, six 5 to 8 month-old calves showed typical signs of md. they all died or were killed in extremis after 2-8 days with progressively worsening clinical signs. post mortem lesions were examined in one calf. non-cytopathogenic md virus was isolated from serum or tissues from 3 clinically affected calves and from 1 healthy heifer. all cows and heifers except for th ... | 1989 | 2629507 |
| genetic comparison of ovine and bovine pestiviruses. | viral rna oligonucleotide fingerprinting was used to compare genetic relationship among pestiviruses originating from ovine or bovine host species. ovine pestiviruses, including reference border disease virus and 2 border disease isolates originating from natural pestivirus infections of sheep, appeared to have a more distant genetic relationship among themselves than with certain bovine pestiviruses. a closer genetic relatedness was evident between border disease virus and 3 noncytopathic bovin ... | 1990 | 1964772 |
| induced sero-conversion in heifers with a field strain of bovine pestivirus--a comparison of methods and doses. | losses from pestivirus infection in a closed herd of cattle occurred over several years. in order to prevent further losses, controlled exposure of non-pregnant heifers to pestivirus from viraemic carrier animals was undertaken. two initial experiments were conducted using either intra-nasal edta blood or field contact. subsequently, other yearling heifers were inoculated with various dilutions of serum using subcutaneous, conjunctival and intra-nasal routes. effective doses were determined. nei ... | 1990 | 1964776 |
| pestivirus infections in ruminants other than cattle. | pestiviruses infect a wide range of domestic, captive and free-living ruminants. among domestic livestock, border disease virus is a well recognised cause of an important congenital disease of sheep in virtually all sheep-rearing countries of the world. the clinical signs, pathogenesis, diagnosis, epidemiology and control of this disease are described in detail. one natural outbreak of border disease in domestic goats has been described and there is serological and virological evidence that pest ... | 1990 | 1966719 |
| ruminant pestivirus infection in pigs. | ruminant pestivirus infections of pigs have a worldwide distribution. the prevalence is varied and depends mainly on (i) contact with cattle, (ii) age of pigs and (iii) degree of homology of virus strains used for serology, with field strains of bovine virus diarrhoea virus (bvdv) infecting pigs. emphasis should be laid on sources of bvdv other than cattle, e.g. contaminated vaccines and fetal calf serum. the need for differentiation of pestiviruses (hog cholera, bovine virus diarrhoea and borde ... | 1990 | 1966720 |
| use of biotechnical methods in veterinary medicine. | biotechnological methods offer promising approaches for improved diagnostic and prophylactic purposes. the following biotechnological techniques are used in the institute of virology at the hanover veterinary school:--production of monoclonal antibodies directed against viral and bacteria-specific antigens such as bovine virus diarrhoea virus, classical swine fever (hog cholera) virus, feline leukaemia virus, animal parvoviruses, alphavirus, brucella and francisella--establishment of improved an ... | 1990 | 1966725 |
| incidence, epidemiology and control of bovine pestivirus infections and disease in australia and new zealand. | pestivirus infection of cattle is widespread and common in both australia and new zealand. the majority of adult animals, of the order of 60%, carry antibody. associated disease is almost entirely that resulting from infection in utero. this includes death of the conceptus, at any stage from conception through pregnancy, or, in those which are born as persistently infected carriers, mucosal disease, most commonly in a chronic form. little or no disease is recognised as a result of the post-natal ... | 1990 | 2132147 |
| attempts to characterize bovine viral diarrhea virus isolated from cattle after immunization with a contaminated vaccine. | bovine viral diarrhea virus (bvdv) was isolated from 28 animals with a history of immunization against respiratory disease with a vaccine contaminated with bvdv. the vaccine-derived parental virus strain and the 28 isolates were analyzed using 10 monoclonal antibodies (mabs) directed against different epitopes and antigenic domains on the major envelope glycoprotein of bvdv. none of the isolates displayed a reaction pattern identical with the parental virus. instead, seven different reaction pat ... | 1990 | 2155768 |
| hepatitis c virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. | hepatitis c virus (hcv) is an important human pathogen that is associated with transfusion-related non-a, non-b hepatitis. recently, hcv cdna was cloned and the nucleotide sequence of approximately three-quarters of the virus genome was determined. a region of the predicted polyprotein sequence was found to share similarity with a nonstructural protein encoded by dengue virus, a member of the flavivirus family. we report here that hcv shares an even greater degree of protein sequence similarity ... | 1990 | 2156259 |
| effect of host cell on the in vitro characteristics expressed by two bovine viral diarrhea virus strains. | growth curves, immunofluorescence assays and radioimmunoprecipitation analysis of bovine viral diarrhea virus (bvdv)-specified polypeptides were used to determine the susceptibility of bovine endothelial cells (becs) to infection with strains of bvdv. one strain of the virus, ny1, normally non-cytopathic when grown in bovine turbinate cells (btus), did not parallel this type behavior in becs. virus replication kinetics, morphological changes in infected cells and resolution of viral polypeptides ... | 1990 | 2156373 |
| a study of some pathogenetic aspects of bovine viral diarrhea virus infection. | the cytopathic (cp) tvm-2 strain of bovine viral diarrhea virus (bvdv) induced in calves a severe disease, characterized by the clinical picture which is usually reported for the acute primary infection observed under natural conditions. in contrast, the calves inoculated with a different biotype of bvdv, the non-cytopathic (ncp) new york-1 strain, remained clinically normal with the only evidence of virus replication in these calves being the recovery of the virus from their pharyngeal swabbing ... | 1990 | 2157576 |
| an elisa for detecting pestivirus antigen in the blood of sheep persistently infected with border disease virus. | a monoclonal antibody capture enzyme-linked immunosorbent assay (elisa) has been developed to detect a pestivirus-specific antigen in leucocytes of sheep persistently infected with border disease virus. a blind trial was conducted to compare the specificity of the elisa with conventional tissue culture virus isolation on blood samples from 58 sheep, aged 3 to 48 months. there was total agreement between the two tests; 27 sheep were shown to be bdv-infected. the elisa od values of the positive sa ... | 1990 | 2157728 |
| antibodies to bovine parvovirus acquired by neonatal pigs through ingestion of virus and antibody in the diet. | the ability of pigs to respond immunologically to ingestion of bovine parvovirus (bpv) was tested by feeding 4 cesarean-derived, colostrum-deprived (cdcd) pigs a live virus-contaminated, liquid diet for the first 4 weeks of life. virus-neutralizing (vn) antibodies were detected in the serum of 2 of the 4 pigs when they were 4 weeks old. antibody titer remained at about the same level for several weeks, then decreased during the remainder of the 29-week interval of testing. the relative reactivit ... | 1990 | 2158254 |
| an enzyme-linked immunosorbent assay (elisa) for antibodies to bovine viral diarrhea virus. | a single dilution enzyme-linked immunosorbent assay (elisa) was developed to detect antibodies to bovine viral diarrhea (bvd) virus in cattle sera. viral antigen (nadl strain) was grown in a pig kidney cell line (pk15), and after removal of nuclear debris, was purified by ultracentrifugation through a potassium tartrate cushion. antigen grown in embryonic bovine tracheal epithelial cells was also satisfactory. the test used a high salt buffer to minimize nonspecific reactivity, polyethylene glyc ... | 1990 | 2159672 |
| range of viral neutralizing activity and molecular specificity of antibodies induced in cattle by inactivated bovine viral diarrhea virus vaccines. | the range of neutralizing activity to bovine viral diarrhea (bvd) virus and viral protein specificity of antibodies induced by 3 inactivated vaccines were evaluated by use of samples of sera obtained from 13 cattle 14 days after vaccination. viral neutralizing antibodies wee detected in all cattle to each of 10 noncytopathic and 10 cytopathic isolates of bvd virus. a viral-induced polypeptide (53,000 to 56,000 daltons) was detected by radioimmunoprecipitation with serum from all vaccinates. othe ... | 1990 | 2159737 |
| in vitro interactions of cytokines and bovine viral diarrhea virus in phytohemagglutinin-stimulated bovine mononuclear cells. | bovine viral diarrhea (bvd) virus inhibited phytohemagglutinin (pha)-stimulated bovine peripheral blood mononuclear cell (pbmc) proliferation and bovine interleukin-2 (il-2) production. in the controls, the heat-inactivated bvd virus was not capable of suppressing the pha-stimulated pbmc proliferation. presence of exogenous cytokines, such as purified human il-2, recombinant bovine interleukin-1 (rbovil-1), recombinant bovine il-2, and recombinant human il-6 failed to reverse the bvd virus-induc ... | 1990 | 2161582 |
| intracellular virus-induced polypeptides of pestivirus border disease virus. | intracellular virus specific polypeptides of pestivirus, border disease virus (bdv) in bovine turbinate cells were analysed by radio-immunoprecipitation with specific antisera. eleven viral polypeptides with molecular weights of 220, 165, 118, 84, 66, 58, 55, 53, 45, 37 and 31 kda, respectively, were detected in infected cells. of these, the 165, 118, 84, 66, 58, 55, 53, 45 and 31 kda proteins were found to be glycosylated. comparative studies indicated that the polypeptides induced by bdv share ... | 1990 | 2161595 |
| in vitro production of antibodies to bovine virus diarrhoea virus by peripheral blood mononuclear cells from cattle immunized by infection. | a method for in vitro production of antibodies to bovine virus diarrhoea virus (bvdv) by peripheral blood mononuclear cells (pbmc) was developed. the pbmc were cultured in microtitre plates coated with detergent-solubilized bvdv and the supernatants were tested in an enzyme-linked immunosorbent assay which detects igg antibodies to bvdv. following incubation of pbmc with an optimal concentration of pokeweed mitogen for 5 days, antibodies to bvdv were detected in culture supernatants of pbmc from ... | 1990 | 2162096 |
| molecular cloning and nucleotide sequence of hog cholera virus strain brescia and mapping of the genomic region encoding envelope protein e1. | genomic rna of hog cholera virus (hcv) strain brescia was cloned and sequenced. the nucleotide sequence was deduced from overlapping cdna clones and comprises 12,283 nucleotides. we cloned the complete 3' end of the hcv genome, but could not unequivocally prove that the cdna sequence also completely covers hcv rna at the 5' end. the hcv genome contained one large open reading frame, which spans the viral plus strand rna and encodes an amino acid sequence of 3898 residues with a calculated molecu ... | 1990 | 2162104 |
| specific sequence amplification of bovine viral diarrhoea virus nucleic acid. | bovine viral diarrhoea virus (bvdv) causes infection of cattle worldwide and is a common contaminant of cell cultures in the laboratory. methods of diagnosis for bvdv are time-consuming and inconsistent. we describe the development of an in vitro test based on enzymatic dna amplification with thermus aquaticus dna polymerase of sequences of bvdv cdna reverse transcribed from viral rna. specific sequences were amplified from infected cell cultures and clinical material using laboratory and field ... | 1990 | 2162162 |
| viral protein production in homogeneous and mixed infections of cytopathic and noncytopathic bvd virus. | experiments were conducted to examine dual infection of cultured cells with cytopathic and noncytopathic bovine viral diarrhea virus (bvdv). cell monolayers infected with a noncytopathic bvdv isolate and subsequently superinfected with a cytopathic bvdv isolate were refractive to the cytopathic effects of the cytopathic bvdv isolate, as reported in the literature. immunofluorescence staining of superinfected cultures with monoclonal antibodies specific for the cytopathic or the noncytopathic vir ... | 1990 | 2162163 |
| detection of bovine viral diarrhea virus genome in leukocytes from persistently infected cattle by rna-cdna hybridization. | a bovine viral diarrhea virus (bvdv) cdna library was constructed. one cloned complementary dna sequence was used as a probe to detect bvdv rna by hybridization in infected cell cultures and in mononuclear leukocytes from persistently infected cattle by dot blot and in situ hybridization. the cdna probe hybridized with all cytopathic and noncytopathic bvdv isolates tested. the hybridization results were consistent with results obtained using conventional subculturing and immunofluorescent staini ... | 1990 | 2162729 |
| a blocking elisa to differentiate hog cholera virus antibodies in pig sera from those due to other pestiviruses. | the blocking elisa technique was extended to comparative serology by using 3 different pestivirus strains: hog cholera virus (hcv) alfort strain propagated in pk15 cell line, border disease virus (bdv) aveyron strain in pk15 and bvd nadl** strain in fetal calf kidney (fck) primary cells. rabbit antisera to the alfort hcv strain and aveyron bdv strain were raised for use in the test. a bovine hyperimmune serum to bvd virus was also used for detecting antibodies specific to bvd virus. the elisa wa ... | 1990 | 2163232 |
| the coding region for the 54-kda protein of several pestiviruses lacks host insertions but reveals a "zinc finger-like" domain. | we sequenced cdnas, amplified by the polymerase chain reaction (pcr), which correspond to the carboxy-terminal portion of the 54-kda protein of various cytopathic (cp) or noncytopathic (ncp) pestiviral strains. except for the previously described insertions in two cp strains of the bovine viral diarrhea virus (bvdv), we did not find comparable insertions in this gene in eight pestiviral strains. the predicted amino acid sequences of this 54-kda protein portion contain a conserved cysteine-rich s ... | 1990 | 2164736 |
| the impact of pestivirus on an artificial breeding program for cattle. | after the introduction of pestivirus into a herd undergoing an embryo transfer and artificial insemination program, substantial post-weaning calf losses occurred. the predominant clinical feature was severe respiratory disease, in contrast to the commonly recognised mucosal disease. thirty-one of 76 calves were affected, with a case fatality rate of 58%. all calves which were persistently infected with pestivirus died during the study period. there was a significant association in the surviving ... | 1990 | 2168165 |
| antigenic variations in bovine viral diarrhea viruses detected by monoclonal antibodies. | five murine monoclonal antibodies (mabs) against the nadl strain of bovine viral diarrhea (bvd) virus were developed, identified, and characterized. four of the mabs were directed against a 53-kilodalton (kda) viral protein, and one was specific to a 47-kda polypeptide. competitive radioimmunoassay showed that two mabs were specific to related epitopes of the 53-kda protein, and the other three mabs were each specific to a different epitope. the mabs were used to study heterogeneity among bvd vi ... | 1990 | 2168436 |
| pestivirus infections in norway. epidemiological studies in goats. | during one breeding season, 2335 female goats in 39 herds in different parts of norway were examined for pestivirus infection and for reproductive performance. before breeding, all animals were examined for neutralizing antibodies against the nadl strain of pestivirus, 83 (3.6 per cent) positive animals in 12 herds being demonstrated. the herd prevalences ranged from 1 to 63 per cent. antibody titres varied from 1 in 4 to 1 in 2048. of 1816 females in 30 herds for which post-breeding information ... | 1990 | 2168445 |
| hydrallantois in cows. | 1990 | 2168601 | |
| characterization of a panel of monoclonal antibodies and their use in the study of the antigenic diversity of bovine viral diarrhea virus. | a panel of 40 monoclonal antibodies (mab) specific for bovine viral diarrhea virus (bvdv) was produced, and each mab was characterized and grouped according to its viral protein specificity, immunoglobulin subclass, virus-neutralizing activity, and immunoreactivity with a large collection of bvdv isolates. the mab were found to be specific for 1 of 3 sets of related viral-induced proteins found in cells infected with the singer strain of bvdv. group-1 mab were specific for the 80- and 118-kiloda ... | 1990 | 2168687 |
| elisa detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies. | a panel of monoclonal antibodies was prepared by immunization of balb/c mice with moredun (bd) virus strains. these antibodies were characterized by immunofluorescence and seroneutralization against bd, bvd and hog cholera (hc) virus strains, and radioimmunoprecipitation of bvd-infected cells extracts. the mabs reacting with the majority of the pestivirus strains recognize the 80 kda antigen of the bvd cytophathic strains. the 80 kda antigen of the bvd/osloss virus strain has been cloned and exp ... | 1990 | 2169672 |
| immunohistological detection of bovine viral diarrhoea virus antigen in the central nervous system of persistently infected cattle using monoclonal antibodies. | in a total of 25 cattle persistently infected with bovine viral diarrhoea virus (bvdv) the distribution of viral antigens in the central nervous system was studied. using a panel of monoclonal antibodies (anti pestivirus c16; anti cytophathic bvdv c38; anti cytopathic and non-cytopathic bvdv c42; anti gp53 bvdv ca-1 and ca-3) and the indirect immunoperoxidase technique, bvdv antigen was located exclusively in neurons. predilection sites for viral persistence were cerebral cortex and hippocampus. ... | 1990 | 2169673 |
| pestiviruses: a review. | pestiviruses comprise a group of economically important animal pathogens, namely hog cholera, bovine viral diarrhoea and border disease viruses. the viruses are serologically closely related and share a common host spectrum, i.e. pigs and numerous domestic and wild living ruminants. interspecies transmissions occur frequently. despite some common features in their natural hosts, pathogenesis of pestivirus-induced disease is complex; especially some aspects of highly fatal mucosal disease of catt ... | 1990 | 2169680 |
| pathogenesis of mucosal disease and molecular aspects of bovine virus diarrhoea virus. | studies carried out over three decades, on the pathogenesis and epidemiology of bovine virus diarrhoea virus (bvdv), have provided the basis for our understanding of the aetiology of mucosal disease. experimental reproduction of the disease has demonstrated the mechanism of sequential infection and the role of the two virus biotypes. the need for "homogeneity" between the biotypes, causing mucosal disease, has demonstrated the precision of immunotolerance. the origin of the cytopathogenic biotyp ... | 1990 | 2169681 |
| experimental pestivirus infections in newborn goat kids. | two experiments were carried out in which 37 healthy newborn goat kids were inoculated with a non-cytopathic ovine (bdv) or a cytopathic bovine pestivirus (bvdv) by intramuscular or intracerebral injection. no kids showed signs of disease or gross lesions which could be attributed to these viruses, but inoculated kids had lower mean growth rates than the controls. significant histological changes in the cns of 14 kids were restricted largely to the white matter and consisted mainly of hypercellu ... | 1990 | 2175318 |
| antigenic comparisons of hog cholera virus isolates from europe, america and asia using monoclonal antibodies. | nineteen monoclonal antibodies (mabs) with specificity for hog cholera virus (hcv) were prepared. they were used in an immune binding (peroxidase linked) assay to determine the reaction patterns of hcv isolates from europe, brazil, usa, japan and malaysia, as well as laboratory reference strains of the virus. a further panel of 17 mabs raised against bovine virus diarrhoea virus (bvdv) was included in the study, together with 5 mabs raised against a non-hcv pestivirus of porcine origin. all the ... | 1990 | 2178905 |
| pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer. | neutralizing monoclonal antibodies directed against hog cholera virus (hcv) precipitated two hcv-encoded glycoproteins, hcv gp55 and hcv gp33. immunoassay with bacterial fusion proteins and western immunoblotting with extracts from infected cells revealed that the antibodies recognized only hcv gp55. coprecipitation of hcv gp33 was shown to be due to intermolecular disulfide bridges. one of the antibodies also reacted with the major glycoprotein of another pestivirus, bovine viral diarrhea virus ... | 1990 | 2370675 |
| cytopathic and non-cytopathic biotypes of border disease virus induce polypeptides of different molecular weight with common antigenic determinants. | ten monoclonal antibodies have been raised against lysates of cells infected with cytopathic border disease virus (bdv). these antibodies all recognize non-cytopathic bdv and react with a number of different strains of bovine viral diarrhoea virus (bvdv). studies with radiolabelled cell lysates show that all the antibodies precipitate two polypeptides of apparent mr 80,000 and 130,000 from cells infected with cytopathic virus and a single polypeptide of apparent mr 120,000 from cells infected wi ... | 1990 | 1693167 |
| monoclonal antibodies to the p80/125 gp53 proteins of bovine viral diarrhea virus: their potential use as diagnostic reagents. | monoclonal antibodies reactive to the bovine viral diarrhea virus (bvdv) protein gp53 were produced and characterized. these antibodies and our panel of anti-p80/125 monoclonal antibodies were tested for their cross-reactivity with 11 different north american and european (danish) bvdv strains and isolates including viruses of both cytopathic and noncytopathic biotypes. the four anti-gp53 monoclonal antibodies were neutralizing for the homologous danish cytopathic isolate and cross-reacted with ... | 1990 | 1696159 |
| an elisa detecting antibody to conserved pestivirus epitopes. | a monoclonal antibody based competition-elisa is described for the detection of pestivirus antibodies directed against conserved epitopes on the p80 viral protein. the elisa detected increases in serum antibody following experimentally induced infections of pigs, cattle and sheep with a wide range of pestiviruses, although the sensitivity of the test was not uniform for the different viruses studied. the elisa was compared with virus neutralization tests for the assessment of porcine, bovine and ... | 1991 | 1713919 |
| variable and hypervariable domains are found in the regions of hcv corresponding to the flavivirus envelope and ns1 proteins and the pestivirus envelope glycoproteins. | based on the flavi- and pestivirus model of genome organization for the hepatitis c virus (hcv) (1-5), the nucleotide and deduced amino acid sequences of the putative envelope (e1) and the junction between the e1 and ns1/envelope 2 (e2) region from six different human isolates of hcv were compared with the nucleotide and predicted amino acid sequences of the prototype hepatitis c virus (hcv-1) (5). the overall percentage of nucleotide and amino acid changes among all six isolates, including hcv- ... | 1991 | 1846505 |
| molecular structure of the japanese hepatitis c viral genome. | the amino acid sequence of the polyprotein deduced from the nucleotide sequence of the japanese hepatitis c virus genome (n. kato et al. (1990) proc. natl. acad. sci. usa 87, 9524-9528) indicated that this virus is a member of a new class of positive-stranded rna viruses. several domains of this polyprotein also showed weak homology with those of flaviviruses and pestiviruses including the chymotrypsin-like serine proteinase, ntpase and rna-dependent rna polymerase. | 1991 | 1849488 |
| hog cholera virus: molecular composition of virions from a pestivirus. | virions from hog cholera virus (hcv), a member of the genus pestivirus, were analyzed by using specific antibodies. the nucleocapsid protein was found to be a 14-kda molecule (hcv p14). an equivalent protein could also be demonstrated for virions from another pestivirus, bovine viral diarrhea virus. the hcv envelope is composed of three glycoproteins, hcv gp44/48, gp33, and gp55. all three exist in the form of disulfide-linked dimers in virus-infected cells and in virions; hcv gp44/48 and gp55 e ... | 1991 | 1870198 |
| [use of monoclonal antibodies for the differential diagnosis of pestivirus infections in swine]. | monoclonal antibodies (mab) specific for hog cholera virus (hcv), bovine viral diarrhoea virus (bvdv) or pestivirus were applied for the differential diagnosis of pestivirus infections in pigs. field virus isolated from 8 confirmed classical swine fever outbreaks and one suspect case was propagated in pk(15) cell cultures and identified by direct immunofluorescence (ifa) and peroxidase linked antibody (pla) assays. peroxidase-linked hcv, bvdv and pestivirus specific mab were applied in direct pl ... | 1991 | 1648800 |
| pestivirus gene expression: the first protein product of the bovine viral diarrhea virus large open reading frame, p20, possesses proteolytic activity. | the positive-strand rna genome of pestiviruses contains a single large open reading frame (orf) extending its entire length and is capable of encoding 450 kda of protein. studies have been undertaken with the purpose of elucidating the specific mechanisms involved in the biogenesis of the complete complement of pestivirus proteins. here, we report on gene expression at the 5' end of the genome of the prototype pestivirus, bovine viral diarrhea virus (bvdv). we demonstrate, using both a cell-free ... | 1991 | 1649345 |
| outbreaks of border disease in goats induced by a pestivirus-contaminated orf vaccine, with virus transmission to sheep and cattle. | five herds with a total of 276 female goats experienced severe outbreaks of reproductive failure due to a pestivirus-contaminated experimental orf vaccine given early in the breeding season. the reproductive failures comprised barrenness in 42 goats, abortion in 53 and, in 118, the birth of dead or weak kids. the incidence of female goats with such failures was 82 per cent overall, herd incidence rates ranging from 79 to 96 per cent. no progeny showed characteristic signs of border disease (bd). ... | 1991 | 1650802 |
| pestivirus bovine viral diarrhea virus polypeptides: identification of new precursor proteins and alternative cleavage pathways. | the genome of pestivirus bovine viral diarrhea virus (bvdv) contains a single 11,964 nt long open reading frame (orf) that is capable of encoding a 449 kda putative polyprotein. although previous studies have described many virus-coded polypeptides that are believed to arise by post- and/or co-translational proteolytic processing in infected cells, there are two separate regions in the orf for which no polypeptide products could be identified. in the present study using site specific antisera, w ... | 1991 | 1651034 |
| pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing. | bovine viral diarrhea virus (bvdv), the prototypic pestivirus, possesses a positive-strand rna genome with a single large open reading frame (orf) encoding about 4000 amino acids. we have endeavored to elucidate the mechanisms involved in protein biogenesis by this pestivirus. here, we present our studies on gene expression from the viral nonstructural protein coding region encompassing the carboxy-terminal 60% of the orf. previous sequence and modeling analyses predicted the amino-terminal regi ... | 1991 | 1651596 |
| prevalence of ruminant pestivirus infections in namibia. | following several clinical cases of suspected bovine virus diarrhoea (bvd) on three namibian cattle farms, a serological survey was conducted on bovine, ovine, caprine and wild ruminant sera originating from different regions of the country. neutralizing antibodies to bvd virus (bvdv) were detected in 58% of 1,014 cattle sera, 14% of 618 sheep sera and 4.6% of 1,118 goat sera. sera from seven of ten wildlife species were positive with kudu, eland and giraffe having prevalence rates greater than ... | 1991 | 1652725 |
| a monoclonal antibody capture elisa to detect antibody to border disease virus in sheep serum. | an enzyme-linked immunosorbent assay (elisa) has been developed to detect antibody to border disease virus (bdv) in sheep serum. a monoclonal antibody bound to 96-well microplates was used to capture antigen from detergent-solubilised bdv-infected cells. single dilutions of test sera were then added to wells containing bound bdv antigen and control wells containing uninfected cell lysates. specific antibody to bdv was detected by an anti-ovine igg antiserum conjugated with horseradish peroxidase ... | 1991 | 1659026 |
| specific sequence amplification of bovine virus diarrhea virus (bvdv) and hog cholera virus and sequencing of bvdv nucleic acid. | the pestiviruses are small enveloped rna viruses and are causative agents of economically important animal diseases in cattle, swine, sheep and goats worldwide. we used the polymerase chain reaction to amplify one common fragment of several different strains of both hog cholera virus and bovine virus diarrhea virus (bvdv). the fragment is located at the 5'-end of the genome immediately upstream of the open reading frame. this is a highly conserved region among the different published pestivirus ... | 1991 | 1659027 |
| pestivirus infections in norway. serological investigations in cattle, sheep and pigs. | serum samples from 1,133 dairy cows (187 herds), 3,712 ewes (103 flocks) and 1,317 adult pigs (877 herds), were tested for neutralizing antibodies against the nadl strain of bovine virus diarrhoea virus. the prevalence rate of seropositive animals was 18.5% in cattle, 4.5% in sheep and 2.2% in pigs, such seroreactors being found in 28% of the cattle herds and 18% of the sheep flocks. in all three species the rate showed considerable herd and geographical variation. in cattle the seroreactor rate ... | 1991 | 1659160 |
| an antigen-capture elisa detects pestivirus antigens in blood and tissues of immunotolerant carrier cattle. | an antigen-capture elisa was developed for the detection of pestivirus-specific antigens in peripheral blood leucocytes (pbls), blood clots and tissue samples of immunotolerant cattle persistently infected with virus. the elisa demonstrated complete agreement with conventional virus isolation procedures undertaken on specimens from a total of 58 carrier animals and 360 uninfected animals. the technique is based on capturing antigen with a high-titred goat polyclonal antiserum and detecting the b ... | 1991 | 1659578 |
| hepatitis c virus. | hepcv is the major cause of nanb pt hepatitis and is also implicated as the cause in a large proportion of sporadic cases of nanbh. chronic infection with hepcv has also been linked to the development of hepatocellular carcinoma. chimpanzees and marmosets are the only animals found to be experimentally infectable and the virus has not been propagated in any cell culture system. hepcv is an enveloped virus with a diameter of 30-60 nm and a 10-kb positive-stranded rna genome. its genome organizati ... | 1991 | 1659796 |
| pestivirus antibodies in pigs in ireland. | 1991 | 1660192 | |
| the development of an international reference panel of monoclonal antibodies for the differentiation of hog cholera virus from other pestiviruses. | a panel of 30 monoclonal antibodies was defined and characterized with respect to the binding capacity in immunoperoxidase assay to different strains of pestivirus. using the panel it was possible to identify specifically all strains and isolates of hog cholera virus, hog cholera vaccines derived from 'c' strains, and most strains of bovine viral diarrhoea/border disease (bvd/bd) viruses (including those isolated from pigs). a small proportion of bvd/bd isolates from pigs and ruminants reacted o ... | 1991 | 1660637 |
| experimental pestivirus infections in pregnant goats. | fifty pregnant goats, inoculated intramuscularly at different gestational stages with a non-cytopathic ovine pestivirus or a cytopathic bovine pestivirus, all developed pestivirus-neutralizing antibodies within 5 weeks of inoculation. the incidence of reproductive failure was similar for the two agents. parturition at term with only healthy kids occurred in 13 (26 per cent) of the goats. viable kids were not born to any of the 17 goats inoculated at about day 40 of gestation. three of the 17 del ... | 1991 | 1663955 |
| viral contamination of fetal bovine serum used for tissue culture: risks and concerns. | four viral contaminants have been routinely detected in unprocessed and commercial lots of fetal bovine serum: bacteriophage, infectious bovine rhinotracheitis, parainfluenza-3 and bovine viral diarrhea virus (bvdv). of those, bvdv is consistently present in a majority of commercial lots of fetal bovine serum. methods for bvdv detection and removal are reviewed. the tentative role of an unclassified pestivirus in microcephaly of infants has been reported. its significance remains uncertain. | 1991 | 1665460 |
| a monoclonal elisa for bovine viral diarrhoea pestivirus antigen detection in persistently infected cattle. | detection of cattle persistently infected with bovine viral diarrhoea virus (bvdv) is crucial to controlling mucosal disease. a sandwich enzyme-linked immunosorbent assay (elisa) using monoclonal antibodies raised against the 48-kda glycoprotein and the 120/80-kda protein was developed for detecting antigens in leucocytes of 3 persistently bvdv-infected calves. the test is simple, sensitive and rapid. moreover the same elisa was able to recognise belgian field isolates of bvdv. these results sho ... | 1991 | 1667787 |
| pestiviruses--taxonomic perspectives. | the history of pestivirus taxonomy is surprisingly consistent: almost 30 years ago it was recognized that pestiviruses are structurally akin to flaviviruses, and recent nucleotide sequence data have confirmed this resemblance at the level of genome organization. for other enveloped positive stranded rna viruses with ikosahedral nucleocapsids e.g. equine arteritis and lactate dehydrogenase virus of mice a taxonomic dilemma is encountered; while virions resemble ("non-arthropod-borne") togaviruses ... | 1991 | 9210920 |
| bovine viral diarrhea virus genomic organization. | in previous work, we developed a preliminary description of the genetic organization of the prototypic pestivirus bovine viral diarrhea virus (bvdv). in order to refine this genetic map and to further elucidate the gene products and expression strategy of this virus, we have generated a broad panel of sequence-specific antibody reagents. use of these reagents not only allowed the identification of several previously undescribed viral polypeptides, but when used in in vivo pulse-chase experiments ... | 1991 | 9210922 |
| bvd monoclonal antibodies: relationship between viral protein specificity and viral strain specificity. | seventeen monoclonal antibodies raised against bovine viral diarrhoea virus were divided into three groups on the basis of radioimmunoprecipitation results. seven monoclonal antibodies precipitated a polypeptide of 80kd and defined four domains, all of which showed considerable conservation amongst the 180 pestivirus strains and isolates examined. nine monoclonal antibodies, including six with virus neutralizing activity, precipitated a 53kd polypeptide and all appeared to be directed towards a ... | 1991 | 9210925 |
| cytopathogenicity of pestiviruses isolated post mortem from cattle. | cytopathic pestivirus was isolated from different tissues of only eight of 23 cattle with mucosal disease. three persistently infected cows were healthy until slaughter after death of all their seven offspring, out of which one of four examined demonstrated cytopathic pestivirus. | 1991 | 9210927 |
| diaplacental infections with ruminant pestiviruses. | pestiviruses are capable of causing diaplacental infections. maternal viremias are important for localizing virus in the ruminant placentome. placental lesions occur with cytopathic bvdv and noncytopathic bdv. the ruminant fetus is very susceptible to pestivirus infections once the virus crosses the placenta because the fetus is 1) agammaglobulinemic, 2) immunologically immature, and 3) it has many immature organ systems with undifferentiated cells. cytopathic bvdv (nadl) in calves and noncytopa ... | 1991 | 9210928 |
| border disease of sheep--aspects for diagnostic and epidemiologic consideration. | border disease (bd) is a condition of newborn sheep that results from congenital infection by a non-cytopathic pestivirus, occurring during the first half of gestation. the variations in expression of the virus directly relate to the age of the fetus at the time of infection. there are four distinct disease syndromes: (1) early embryonic death, (2) abortion and stillbirth, (3) birth of lambs with malformations, and (4) birth of small, weak lambs, lacking characteristic clinical signs, but bearin ... | 1991 | 9210930 |
| identification and production of pestivirus proteins for diagnostic and vaccination purposes. | using a panel of monoclonal antibodies (mabs) previously characterized by seroneutralization, immunofluorescence and radioimmunoprecipitation, we have identified pestivirus proteins useful for diagnostic purposes from the cytopathic osloss isolate of bovine viral diarrhea virus (bvdv). proteins that should be useful for vaccination have also been analysed. cell-free translation of rna from glycoprotein-coding cdna fragments produced, when synthesized in the presence of canine pancreatic microsom ... | 1991 | 9210936 |
| identification of cattle infected with bovine virus diarrhoea virus using a monoclonal antibody capture elisa. | a monoclonal antibody capture enzyme linked immunosorbent assay (elisa) has been developed to detect pestivirus-specific antigen in the leucocytes of cattle infected with bovine virus diarrhoea virus (bvdv). a blind trial was conducted to compare the specificity of the elisa with conventional tissue culture virus isolation on 215 blood samples submitted for bvdv diagnosis from cattle throughout scotland. one hundred and sixty seven samples were negative by both elisa and virus isolation and 47 s ... | 1991 | 9210938 |
| cdna probes for the detection of pestiviruses. | probes were prepared from genomic rna of hog cholera virus (hcv) after synthesis of cdna and cloning. six probes were selected according to their place on the viral genome determined by sequencing and comparison with bvdv sequence. these probes were hybridized with two strains of hcv (alfort and nord), two strains of bovine viral diarrhea (bvdv) (nadl, new york) and four strains of border disease (bd) (lyon 1, lyon 2, aveyron, iemvt). this panel of six probes seem to be able to differentiate pes ... | 1991 | 9210941 |
| polymerase chain reaction amplification of segments of pestivirus genomes. | reverse transcription followed by polymerase chain reaction (pcr) amplification of a region of the viral genome at the 3' end of the glycoprotein(s) gene was employed with the aim of determining its applicability as a diagnostic tool for pestiviruses. candidate primers were designed, from homologous segments detected by comparison between the sequences of strains nadl, osloss and alfort. a segment of 634 base pairs on the glycoprotein gene was targeted for amplification. segments of five pestivi ... | 1991 | 9210946 |
| production of monoclonal antibodies to study the molecular biology of bovine viral diarrhea virus. | five monoclonal antibodies produced against bovine viral diarrhea virus were characterized for some of their biological activities. all of them bound to varying degrees to pestivirus strains but failed to neutralize the virus. one of the antibodies immunoprecipitated four polypeptides presumably involved in viral envelope organization. | 1991 | 9210947 |
| ruminant pestivirus infections. virology, pathogenesis, and perspectives of prophylaxis. june 1990, hannover, germany. symposium proceedings. | 1991 | 9229506 | |
| a "zinc finger-like" domain in the 54kda protein of several pestiviruses. | 1991 | 9229507 | |
| importance of primer selection for the detection of hepatitis c virus rna with the polymerase chain reaction assay. | we compared four primer sets from conserved regions of the hepatitis c virus (hcv) genome for their ability to detect hcv rna in a "nested" cdna polymerase chain reaction assay on sera from 114 anti-hcv antibody-positive individuals from around the world. the different primer sets had equivalent sensitivity, detecting less than 1 chimpanzee id50 (dose that infects 50%) when tested against reference strain h of hcv. we tested equal amounts of rna extracted from the serum of each individual with t ... | 1992 | 1309604 |
| heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses. | in order to analyse the expression of the non-structural (ns) protein p80/p125 in cells infected with different pestiviruses at the protein level, radioimmunoprecipitations with the pestivirus-specific monoclonal antibody (mab) bvd/c16 were performed. cell lysates infected with cytopathic (cp) and non-cytopathic (ncp) bovine viral diarrhoea (bvd) virus strains and isolates, and with hog cholera (hc) virus strains were analysed. from cpbvd virus-infected cells, the mab precipitated one or more pr ... | 1992 | 1309861 |
| the pestiviruses. | 1992 | 1315479 | |
| pathomorphological and immunohistological findings in progeny of goats experimentally infected with pestiviruses. | a total of 25 pregnant goats without neutralizing antibodies against bvd virus were inoculated with two different pestivirus isolates at eight different stages of gestation. in both infection groups, various malformations were observed in fetuses and neonates. in three twins with neutralizing antibodies against bvd virus leukoencephalomalacia occurred, characterized by gelatinous transformation in the cerebral hemispheres. these lesions were comparable to alterations described in alternative pat ... | 1992 | 1316022 |
| sequence analysis of the 5' noncoding region of hepatitis c virus. | we have determined the nucleotide sequence of the 5' noncoding (nc) region of the hepatitis c virus (hcv) genome in 44 isolates from around the world. we have identified several hcv isolates with significantly greater sequence heterogeneity than reported previously within the 5' nc region. the most distantly related isolates were only 90.1% identical. nucleotide insertions were seen in three isolates. analysis of the nucleotide sequence from 44 hcv isolates in this study combined with that of 37 ... | 1992 | 1317578 |
| baculovirus expression of pestivirus non-structural proteins. | bovine viral diarrhoea virus (bvdv) belongs to the pestivirus group, a genus within the flaviviridae family. it possesses a positive-sense ssrna genome with a single large open reading frame (orf) encoding about 4000 amino acids. here we report the continuation of our studies of pestivirus protein biogenesis, involving expression from the viral non-structural protein-encoding region. the 3'-terminal 60% of the bvdv orf was cloned into a plasmid transfer vector which was then used to construct a ... | 1992 | 1321220 |
| protection of piglets born from ruminant pestivirus experimentally infected sows, and their contacts, to the challenge with hog cholera virus. | two groups, a and b, of two specific pathogen-free pregnant sows were experimentally infected between the 25th and 29th days post-breeding with two strains of ruminant pestivirus: nadl cytopathic bovine viral diarrhoea virus for group a and aveyron non-cytopathic border disease virus french strain for group b. two other pregnant sows (group c) were kept uninoculated as control. when 7 weeks old, 8 piglets of group c were put in contact with 4 piglets of group a (group d), and 8 other piglets of ... | 1992 | 1324630 |
| molecular genetics of pestiviruses. | recent developments in understanding the molecular genetics of pestiviruses are reviewed. the genomic rna of several pestiviruses has been molecularly cloned and sequenced. the genetic organization of the viral protein coding region has been refined, and the complete complement of virus-encoded proteins has been elucidated. viral gene expression involves both co-translational and post-translational precursor polyprotein processing. biogenesis of the virion structural proteins requires the proteo ... | 1992 | 1325329 |
| genetic diversity and bvd virus. | the various measures of genetic variation of bvd virus was reviewed with emphasis on the implications for future control of virus-induced disease and diagnosis. while experimental data does not support unique serotypes for bvdv, there is substantial antigenic variation among the isolates examined. this variation may permit fetal infections even in animals assumed to be well vaccinated. the genetic differences between cytopathic and noncytopathic strains of bvdv are expressed in infected cells by ... | 1992 | 1325330 |
| border disease of sheep: the disease in the newborn, adolescent and adult. | border disease (bd) is a condition of newborn sheep that results from congenital infection by a non-cytopathic pestivirus occurring during the first half of gestation. the expression of the virus is largely determined by the age of the fetus at the time of infection, producing four distinct disease syndromes: (1) early embryonic death, (2) abortion and stillbirth, (3) birth of lambs with malformations, and (4) birth of small, weak lambs, lacking characteristic clinical signs, but bearing feature ... | 1992 | 1325332 |
| the hog cholera virus. | hog cholera virus (hcv) is a spherical enveloped particle of about 40-60 nm dia. the viral genome is a single strand rna of about 12,000 bases with positive polarity. one single large open reading frame codes for presumably four structural, i.e. three glycoproteins and a core protein, and about three to five nonstructural proteins. the functional role is not yet fully clear for all viral proteins. hcv belongs to the pestivirus group and it is closely related to bovine viral diarrhoea and border ... | 1992 | 1325334 |
| secondary structure of the 5' nontranslated regions of hepatitis c virus and pestivirus genomic rnas. | the rna genomes of human hepatitis c virus (hcv) and the animal pestiviruses responsible for bovine viral diarrhea (bvdv) and hog cholera (hchv) have relatively lengthy 5' nontranslated regions (5'ntrs) sharing short segments of conserved primary nucleotide sequence. the functions of these 5'ntrs are poorly understood. by comparative sequence analysis and thermodynamic modeling of the 5'ntrs of multiple bvdv and hchv strains, we developed models of the secondary structures of these rnas. these p ... | 1992 | 1329037 |