Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| processing of the dengue virus type 2 proteins prm and c-prm. | a glycoprotein c-prm of 35,000 m(r) was immuno-precipitated from lysates of aedes albopictus cells infected with dengue virus type 2 (den-2) using antisera directed against the c protein or an amino-terminal fragment of the prm glycoprotein. c-prm was not detected in infected vero cells. the prm glycoprotein synthesized in infected a. albopictus and vero cells was cleaved to produce the membrane-associated virion protein (m) and the non-m fragment (pr) immediately preceding or occurring simultan ... | 1993 | 8429301 |
| dengue 2 virus ns2b and ns3 form a stable complex that can cleave ns3 within the helicase domain. | flavivirus genomic rna is translated into a large polyprotein that is processed into structural and nonstructural proteins. the n-termini of several nonstructural proteins are produced by cleavage at dibasic sites by a two-component viral proteinase consisting of ns2b and ns3. ns3 contains a trypsin-like serine proteinase domain at its n-terminus, whereas the function of ns2b in proteolysis is yet to be determined. we have used an ns3-specific antiserum, under nondenaturing conditions, to demons ... | 1993 | 8460492 |
| direct sequencing of genomic cdna fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses. | a nucleotide fragment encoding amino acids 29 to 94 in the e-protein of 28 dengue-2 isolates of diverse geographic and host origins was examined by direct sequencing of a polymerase chain reaction (pcr)-amplified product, and compared to six previously published sequences. nucleotide divergence ranged from 0 to 19.8% corresponding to a maximum of 9% divergence in the amino acid sequence. taking a divergence of 6% between the nucleotide sequence as a cut off for genotype classification, six group ... | 1993 | 8470950 |
| sequence of the dengue virus type 2 (strain pr-159) ns1 gene and comparison with its vaccine derivative. | 1993 | 8479921 | |
| selection and partial characterization of dengue 2 virus mutants that induce fusion at elevated ph. | two types of dengue (den) 2 virus mutants were selected either by repeated exposure to acidic ph (acid mutant, am), or by the addition of ammonium chloride to aedes albopictus c6/36 cells prior to and during viral infection (fusion mutant, fm). both mutants grew more slowly than the parent strain and induced smaller plaques in vero cells. the 50% fusion from within index for both mutants occurred at least 0.65 ph units higher than with the wild-type den virus. a single amino acid substitution (a ... | 1993 | 8480420 |
| protective efficacy in mice of a secreted form of recombinant dengue-2 virus envelope protein produced in baculovirus infected insect cells. | we constructed a recombinant baculovirus encoding a dengue (den)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its c-terminus (d2e delta 102). the production, processing and transportation of the recombinant protein in baculovirus-infected spodoptera frugiperda (sf9) cells and its immunogenic properties in mice were compared to those of a previously characterized recombinant den-2 e-protein with a 71aa c-terminal truncation (d2e delta 71). both proteins were transported thro ... | 1994 | 7826210 |
| comparative nucleotide and deduced amino acid sequence of the envelope glycoprotein gene among three dengue virus type 2 strains isolated from patients with different disease severities in maha sarakham, northeast thailand. | the nucleotide (nt) sequence of the envelope glycoprotein (e) gene of dengue virus type 2 was determined by the primer-extension dideoxy chain-termination method for 3 dengue virus type 2 (d2) strains which had been isolated from patients with dengue fever (df), dengue hemorrhagic fever (dhf), and dengue shock syndrome (dss), in maha sarakham, northeast thailand, in 1986-1987. their nt sequences were essentially the same except for a single silent nt replacement in each dhf and dss strain compar ... | 1994 | 7855635 |
| the first epidemic of dengue hemorrhagic fever in french guiana. | from july 1991 to october 1992, an outbreak of dengue spread into the main urban areas of french guiana, where 90% of the country's 114,808 inhabitants live. in mid-july 1991 dengue-2 virus was identified as being responsible for most cases, while dengue-1 virus was rarely isolated and circulated at a low level. the number of dengue cases during this period was unknown because there was no clinically based dengue surveillance system. the only available data were for the number of suspected cases ... | 1994 | 7985746 |
| use of a flavivirus rna-dependent rna polymerase assay to investigate the antiviral activity of selected compounds. | we have developed an assay using flavivirus rna-dependent rna polymerase to test the inhibitory activity of potential antiviral agents. the effects of antiviral agents on rna synthesis were examined in this assay using extracts of vero cells infected with dengue virus type 2 or kunjin virus. several different classes of known polymerase inhibitors were tested. the synthesis of double-stranded replicative form rna was inhibited in a dose-dependent fashion in the presence of the polyoxometalate hp ... | 1994 | 7993077 |
| analysis of c-terminally truncated dengue 2 and dengue 3 virus envelope glycoproteins: processing in insect cells and immunogenic properties in mice. | we constructed two recombinant autographa californica nuclear polyhedrosis baculoviruses. spodoptera frugiperda (sf9) cells containing these constructs produce carboxy-terminally truncated envelope e proteins representing dengue (den) virus serotypes 2 and 3. the two recombinant proteins contained their homologous signal sequences at the n terminus and were truncated by 71 and 74 amino acids at the c terminus, respectively. this allowed the translocation of the recombinant proteins to the endopl ... | 1994 | 8021588 |
| dengue type-2 virus envelope protein made using recombinant baculovirus protects mice against virus challenge. | the gene coding for the envelope (e) glycoprotein of dengue-2 virus was cloned into baculovirus (autographa californica nuclear polyhedrosis virus). the recombinant virus contained the entire e protein gene, preceded by 38 nucleotides from the end of the prematrix glycoprotein gene and followed by the first 83 nucleotides of nonstructural protein 1. when expressed in spodoptera frugiperda (sf9) cells, approximately 1 mg of recombinant e antigen was made per 10(9) cells. this antigen reacted with ... | 1994 | 8147490 |
| dengue infection complicated by severe hemorrhage and vertical transmission in a parturient woman. | a thai woman with a febrile illness was delivered of a healthy infant by cesarean section. the etiology of her 2-day fever was unknown at the time of surgery; results of serology performed later established a diagnosis of dengue infection. postoperative hemostatic monitoring was not performed. she experienced massive and prolonged (8 days) wound hemorrhage that necessitated multiple transfusions of blood, platelets, and frozen plasma. the newborn became febrile on his 6th day of life. dengue vir ... | 1994 | 8161636 |
| glycosylation mutants of dengue virus ns1 protein. | the non-structural glycoprotein ns1 of dengue virus type 2 contains sites for n-linked glycosylation at asn-130 and asn-207. ns1 synthesized in infected cells is glycosylated at both locations. we have now examined the dimerization and secretion of ns1 lacking one or both of these sites by transient expression of mutagenized cdna inserted into a simian virus 40-based vector. immunoblotting and radioimmunoprecipitation were used to detect ns1 associated with transfected cells and in the extracell ... | 1994 | 8176380 |
| active immunization by a dengue virus-induced cytokine. | dengue type 2 virus (dv)-induced cytotoxic factor (cf) is capable of reproducing various pathological lesions in mice that are seen in human dengue. the present study was undertaken to investigate the protective effect of active immunization of mice with cf. mice were immunized with 5 microgram of cf and prevention of cf-induced increase in capillary permeability and damage to the blood-brain barrier were studied at weekly intervals, up to 48 weeks, by challenging with 3 microgram of cf. maximum ... | 1994 | 8187327 |
| development of a nested-pcr test based on sequence analysis of epizootic hemorrhagic disease viruses non-structural protein 1 (ns1). | two orbiviruses, epizootic hemorrhagic disease (ehd) and bluetongue (btv) viruses, cause disease in domestic and wild ruminant species. the gene that encodes non-structural protein 1 (ns1) of ehd virus, serotype 1, was sequenced and compared to ehd and btv ns1 sequences. the ns1 gene was found to be more conserved than the vp3 gene, and was selected as a target for polymerase chain reaction (pcr) amplification. the ns1 genes of several btv viruses and another orbivirus, african horse sickness (a ... | 1994 | 8191788 |
| nucleotide and deduced amino acid sequences of genes encoding the structural and nonstructural ns1 proteins of a dengue-2 virus isolated in china. | we have determined the nucleotide and encoded amino acid sequences of the capsid, membrane precursor, membrane, envelope, and nonstructural ns1 protein genes of a dengue-2 virus (d2-04) isolated from a patient in hainan, china. the sequenced region contains a gene organization similar to that of other flaviviruses. the overall amino acid sequence similarity between d2-04 and other dengue-2 viruses is greater than 92%, whereas that between d2-04 and members of the other dengue serotypes is about ... | 1994 | 7911607 |
| dengue virus-specific memory t cell responses in human volunteers receiving a live attenuated dengue virus type 2 candidate vaccine. | a live attenuated dengue virus type 2 candidate vaccine (16681-pdk53) was evaluated in a phase i trial in 10 nonimmune adult volunteers. the dengue virus-specific memory t cell responses were analyzed as part of this study. dengue virus-specific t cell proliferative responses were observed in all subjects after stimulating their peripheral blood mononuclear cells with live viruses or noninfectious viral antigens. the highest proliferative response was against dengue virus type 2, although cross- ... | 1994 | 7912253 |
| synaptophysin and neurofilament expression in neurons infected with dengue virus. | the expression of two genes encoding the neuronal specific proteins synaptophysin and high molecular weight neurofilaments was investigated in primary cell cultures of embryonic mouse brain infected with dengue virus type 2, using immunocytochemistry and western blot analysis with monoclonal antibodies. the viral infection leads to a 20-fold induction in the expression of the mentioned synaptogenesis-related proteins. these results suggest a correlation between virus infection and neuropathology ... | 1994 | 7919816 |
| phenotypic characteristics of dengue 2 virus persistently infected in a c6/36 clone of aedes albopictus cells. | a c6/36 cell culture persistently infected by dengue 2 virus was established and retained for over 20 weeks. no cpe was observed at any stage of infection. however, some giant cells up to 2- or 3-fold the diameter of normal cells appeared in the established culture. extracellular virus titers fluctuated with a periodic tendency while the antigen level remained constant, suggesting some viral particles evolved into noninfectious defective interfering particles. plaque size tended to change during ... | 1994 | 7928286 |
| precise location of sequential dengue virus subcomplex and complex b cell epitopes on the nonstructural-1 glycoprotein. | the reactions of a panel of 34 mouse monoclonal antibodies (mabs) specific for the dengue-2 virus nonstructural-1 glycoprotein (ns1), were analysed using 174 overlapping synthetic nonameric peptides covering the entire sequence. using this methodology, four epitopes were identified. one pair of mabs, which defined a dengue-2/4 virus subcomplex epitope (24c: amino acids 299-309) using native ns1 proteins, showed the same reaction pattern with synthetic peptides containing the corresponding ns1 se ... | 1994 | 7944953 |
| t-helper cell epitopes on the e-glycoprotein of dengue 2 jamaica virus. | to identify t-helper (th)-cell epitopes, we analyzed 25 synthetic peptides, which included most of the 495-amino-acid sequence of the envelope (e)-glycoprotein of dengue 2 virus. the peptides were analyzed in three mouse strains, balb/c (h-2d), c57bl/6 (h-2b), and outbred nih-swiss, for their ability to elicit antibody or prime the th-cell compartment following two inoculations in freund's incomplete adjuvant. sixteen peptides were able to elicit an antipeptide antibody response in one or more m ... | 1994 | 7505071 |
| restriction enzyme analysis of american region dengue viruses. | restriction fragment heterogeneity of hae iii digestion products of cdna to virion rna was used to map the distribution of dengue virus topotypes found in the american region. by comparing the electrophoretic patterns of fragments produced, dengue virus isolates were placed in groups that agreed with those previously determined by oligonucleotide fingerprinting. dengue-1 and dengue-4 viruses occur throughout the western hemisphere as single genetic types, with most of the isolates sharing at lea ... | 1994 | 7516146 |
| localization of a neutralizing epitope on the envelope protein of dengue virus type 2. | two neutralization-resistant variants of dengue virus type 2 were selected using the neutralizing monoclonal antibody g8d11. virus n-gv4 was derived from the new guinea c strain and virus p-gv3 from the puo-218 strain. both variants had an identical change at nucleotide 919 in the e gene, causing a substitution of glutamic acid for lysine at residue 307 in the e glycoprotein. the substitution abolished the ability of antibody g8d11 to bind to the e glycoprotein in radioimmunoprecipitation experi ... | 1994 | 7518164 |
| lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes. | the effect of interferon-gamma (ifn-gamma) on dengue virus multiplication in human peripheral blood monocytes was investigated. enriched monocytes were treated with ifn-gamma and then infected with dengue virus type 2 either directly or in the presence of optimal infection-enhancing levels of antibodies. pretreatment of monocytes from dengue-immune donors with 100 iu/ml of ifn-gamma caused 12- to 97-fold and 13- to 137-fold reduction of virus yields at 24 hr after infection in the absence and pr ... | 1995 | 7536230 |
| analysis of functional epitopes on the dengue 2 envelope (e) protein using monoclonal igm antibodies. | forty-two hybridomas secreting igm antibody against dengue virus were derived from spleen cells of dengue 2 infected mice. antibody from 27 of these recognised the e protein of this virus. of the 22 antibodies which neutralised dengue 2, only two cross-reacted with other flaviviruses. these 22 antibodies recognised three discrete domains on dengue virions. competitive binding studies with igg monoclonal antibodies suggested that two of the three domains were recognised by both igg and igm antibo ... | 1995 | 7541621 |
| synthesis and characterization of an infectious dengue virus type-2 rna genome (new guinea c strain). | dengue virus type 2 (den-2), a member of the flaviviridae family, has a positive-strand rna genome, 10,723 nucleotides (nt) in length and encoding a single polyprotein precursor consisting of 3391 amino acids (aa). in order to construct a full-length cdna clone, the viral genome was cloned into 5' (nt 1-2203 under the control of the t7 promoter (pt7)) and 3' (nt 2203-10,723) constructs. a full-length den-2 cdna under pt7 control was assembled in vitro after excising the two cdna inserts from the ... | 1995 | 7557426 |
| cytotoxic factors released by dengue virus-infected human blood monocytes. | human monocyte-derived cytotoxic factors (cf) induced by dengue virus were studied. using several human leukemia cell lines as precursors, the biological activities of cf in conditioned medium from dengue virus-infected monocytes were demonstrated through the measurement of tumor cell growth inhibition. the conditioned medium from dengue virus infected monocytes suppressed significantly growth of cem, hl60, k562, and u937 cells. in the presence of 10% conditioned medium (v/v) from dengue virus i ... | 1995 | 7561793 |
| a model to study cytokine profiles in primary and heterologously secondary dengue-2 virus infections. | roles of cytokines in primary and secondary dengue virus (dv) infections are not completely understood. in this study, we challenged mononuclear leukocytes (mnls) obtained from non-immune and dv-1-infected donors with dv-2 in vitro to mimic primary and heterologously secondary dv-2 infections, respectively. we found that mnls in response to dv-2 could release a large amount of interleukin-1 (il-1 beta) and tumor necrosis factor alpha (tnf alpha). however, il-1 beta and tnf alpha released by mnls ... | 1995 | 7572465 |
| dengue virus-induced human cytotoxic factor: production by peripheral blood leucocytes in vitro. | during dengue type 2 virus (dv) infection of mice a unique cytokine, the cytotoxic factor (cf), is produced which reproduces the pathological lesions seen in patients of dengue haemorrhagic fever (dhf). recently we have observed a cf-like protein in the sera of dhf cases. the present study was undertaken to investigate whether dv can stimulate human peripheral blood mononuclear cells (pbmc) in vitro to produce human cf (hcf). cultures prepared from pbmc or its enriched subpopulations were inocul ... | 1995 | 7586676 |
| association between ns3 and ns5 proteins of dengue virus type 2 in the putative rna replicase is linked to differential phosphorylation of ns5. | dengue virus type 2, a member of the family flaviviridae, encodes a single polyprotein precursor consisting of 3391 amino acids residues that is processed to at least 10 mature proteins by host and viral proteases. the ns3 protein contains a domain commonly found in cellular serine proteinases that in cooperation with ns2b is involved in polyprotein processing. in addition, ns3 and ns5 proteins contain conserved motifs found in several rna helicases and rna-dependent rna polymerases, respectivel ... | 1995 | 7642575 |
| antibodies that block virus attachment to vero cells are a major component of the human neutralizing antibody response against dengue virus type 2. | epidemiological data strongly implicate a role for the host humoral immune response in both protection against and exacerbation of dengue virus-caused disease. in an effort to characterize elements of the normal human immune response against dengue virus we have addressed the issue of antibody-mediated neutralization of dengue virus. we show here the ability of both mouse monoclonal antibody 3h5 and human anti-dengue neutralizing sera to block binding of dengue-2 virus to monkey kidney (vero) ce ... | 1995 | 7666046 |
| antibody-enhanced binding of dengue-2 virus to human platelets. | the mechanisms underlying severe thrombocytopenia in dengue hemorrhagic fever/dengue shock syndrome (dhf/dss) are not completely understood. we present here the first evidence that dengue type 2 virus binds to human platelets only in the presence of virus-specific antibody, supporting a role for immune-mediated clearance of platelets in the pathogenesis of thrombocytopenia in dhf/dss. antibody-enhanced binding of virus of platelets was also demonstrated with a panel of eight murine monoclonal an ... | 1995 | 7483271 |
| murine immunoglobulin g subclass responses following immunization with live dengue virus or a recombinant dengue envelope protein. | murine immunoglobulin g (igg) subclass responses to immunization are restricted to certain subclasses depending on the nature of the immunogen. immunization with live viruses generally leads to a predominant igg2a response, which may be the most effective at resisting future challenge due to the unique effector functions of igg2a. knowledge of subclass responses following immunization with dengue vaccine candidates may be helpful in determining which candidates are most efficacious. we measured ... | 1995 | 7485699 |
| inhibition of dengue virus by novel, modified antisense oligonucleotides. | five different target regions along the length of the dengue virus type 2 genome were compared for inhibition of the virus following intracellular injection of the cognate antisense oligonucleotides and their analogs. unmodified phosphodiester oligonucleotides as well as the corresponding phosphorothioate oligonucleotides were ineffective in bringing about a significant inhibition of the virus. novel modified phosphorothioate oligonucleotides in which the c-5 atoms of uridines and cytidines were ... | 1995 | 7983769 |
| blood-feeding behavior of dengue-2 virus-infected aedes aegypti. | this study was designed to determine if infection of aedes aegypti with dengue-2 virus affects the ability of the mosquito to efficiently locate and imbibe blood from an uninfected host. previous studies suggest that some parasites manipulate their arthropod host to increase the probability of transmission by interfering with blood-feeding efficiency. we found no evidence that dengue-2 virus infection by intrathoracic inoculation impaired the blood-feeding efficiency of ae. aegypti. we speculate ... | 1995 | 7694963 |
| partial nucleotide and amino acid sequences of the envelope and the envelope/nonstructural protein-1 gene junction of four dengue-2 virus strains isolated during the 1981 cuban epidemic. | in 1981, an epidemic of dengue hemorrhagic fever (dhf) caused by dengue-2 virus occurred in cuba. this was the first dhf epidemic reported in the western hemisphere. in this study, we have analyzed four dengue-2 cuban strains for two short genomic fragments: one on the envelope (e) glycoprotein and one at the e/nonstructural protein-1 (ns1) gene junction. the e segment of these 1981 cuban isolates were more closely related to older dengue-2 virus strains such as new guinea c 1944, thailand 1964, ... | 1995 | 7694966 |
| the effect of multiple host contacts on the infectivity of dengue-2 virus-infected aedes aegypti. | this study was designed to determine if transmission rates for dengue-2 virus by aedes aegypti are altered by mosquitos probing a host for blood or imbibing blood prior to attempting transmission. aedes aegypti is known to contact multiple hosts during each egg-laying cycle and multiple host contacts might diminish the amount of virus in infected mosquito's salivary glands or ducts and render them functionally uninfective. probing a host 5, 10, or 20 consecutive times did not significantly alter ... | 1995 | 7707190 |
| monitoring the cdna synthesis of dengue-2 virus by rt pcr. | reverse transcriptase polymerase chain reaction (rt pcr) was utilized to observe the complementary dna (cdna) synthesis from the 10723 base dengue-2 virus template by in vitro reverse transcription. the pcr product amplified from 5'-end of the genome (pcr primers n1a-e1) indicates the completeness of the cdna synthesis because the cdna primer d8b was located at 3'-end and the cdna synthesized encompassed the entire rna template. the integrity of the dengue virus rna was also determined by the cd ... | 1995 | 7730437 |
| a higher production of platelet activating factor in ex vivo heterologously secondary dengue-2 virus infections. | patients with a dengue-1 virus (dv-1) infection followed by another dv-2 infection were reported to have a higher incidence of dengue haemorrhagic fever (dhf). mononuclear leukocytes (mnls) are principal cells for dv replication so that we challenge mnls obtained from non-immune and previously dv-1-infected donors with dv-2 to mimic primary and heterologously secondary dv-2 infections. production of platelet activating factor (paf), thromboxane b2 (txb2), and prostaglandin d2 (pgd2) by mnls in t ... | 1995 | 8689093 |
| dengue type 2 outbreak in the south of the state of bahia, brazil: laboratorial and epidemiological studies. | during march 1994 cases of a exanthematic acute disease were reported in the municipalities of itagemirim, eunápolis and belmonte, state of bahia. dengue fever was confirmed by serology (mac-elisa) and by dengue virus type 2 isolation, genotype jamaica. signs and symptoms of classic dengue fever were observed with a high percentual of rash (73.8%) and pruritus (50.5%). major haemorrhagic manifestations were unfrequent and only bleeding gum was reported. dengue virus activity spreaded rapidly to ... | 1995 | 8731263 |
| flow cytometric analysis of t4:t8 splenic cell during dengue virus infection of mice. | the present study was undertaken to investigate the effect of dengue type 2 virus (dv) and dv-induced cytokines (cf and cf2) on t lymphocyte subpopulations of spleen by flow cytometry. following dv-ic inoculation in mice the percent number of cd4+ and cd8+ lymphocytes in the spleen was reduced, the peak reduction in both was observed on the 6th day post-inoculation (p.i.). intravenous inoculation of cf or cf2 in mice also decreased the percent number of cd4+ as well as cd8+ t lymphocytes subpopu ... | 1995 | 8786153 |
| effect of adjuvants on immunization with dengue virus-induced cytotoxic factor. | specific active immunization with dengue type 2 virus (dv)-induced cytokine, cytotoxic factor (cf), prevents cf-mediated pathology in mice. the present study was undertaken to determine the optimum dose of cf and the effect of different adjuvants on the immune response as assessed by the study of anti-cf antibody titre by elisa and protection against increase in capillary permeability to challenging dose of 3 micrograms cf. the maximum protection of 94 +/- 4% against increase in capillary permea ... | 1995 | 8536363 |
| evaluation of enemas for exposing aedes aegypti to suspensions of dengue-2 virus. | to determine if enemas could be used to simulate oral exposure of aedes aegypti to dengue-2 virus, we compared infection rates of mosquitoes administered an enema of a blood suspension containing dengue-2 virus to infection rates in mosquitoes that imbibed the same suspension from a drop of blood. in 1 of 4 experiments infection rates were significantly different. more importantly, during 2 of the 4 trials, 100% of the mosquitoes in the enema treatment group were infected. we speculate that the ... | 1995 | 8551311 |
| failure of immune sera to neutralize dengue-2 virus in intrathoracically inoculated aedes aegypti. | aedes aegypti became infected when inoculated with a mixture of dengue-2 virus and anti-dengue-2 antibodies, but not when they were exposed to the same mixture per os. this phenomenon merits more detailed investigation. understanding why this difference in mosquito infection rate occurs may lead to improved dengue virus assays or provide insights into the nature of dengue virus-antibody interactions. | 1995 | 8551312 |
| mice immunized with a dengue type 2 virus e and ns1 fusion protein made in escherichia coli are protected against lethal dengue virus infection. | a gene fragment encoding the c-terminal 204 amino acids (aa) from the structural envelope glycoprotein (e) and the n-terminal 65 aa from non-structural protein one (ns1) of dengue type 2 virus (den-2) was expressed in escherichia coli (e. coli) as a fusion protein with staphylococcal protein a. the recombinant fusion protein was purified and analysed for its antigenicity, its immunogenicity and its ability to protect mice against lethal challenge with live den-2 virus. the recombinant protein wa ... | 1995 | 8578812 |
| genotype determination of three dengue type 2 virus strains from myanmar by sequencing e/nsi gene junction. | genotype of three dengue-2 virus strains from myanmar was determined as genotype ii by sequencing 240 nucleotide long fragment across the e/ns1 gene junction by the primer extension dideoxy chain termination method, applying direct sequencing of the pcr product. these strains were isolated from a dengue shock syndrome (dss) patient and two patients with dengue hemorrhagic fever (dhf) grade 1, in yangon (rangoon), myanmar (burma), in 1987. sequence homology of all three strains were highest (96%) ... | 1995 | 9139372 |
| anti-dengue igg detection by an indirect elisa. | protein-free culture media were originally developed for hybridomas to simplify downstream processing and purification. for the same reasons, we have used these protein-free media for passaging dengue 2 virus in c6/36 cells. this provided us with an infected supernatant (denpf) which could then be used as coating antigens for an indirect enzyme-linked immunosorbent assay (elisa) to determine dengue igg levels. using this preparation, the main immunogenic band as seen by immunoblot appeared to be ... | 1995 | 9139374 |
| maturation of the dengue-2 virus ns1 protein in insect cells: effects of downstream ns2a sequences on baculovirus-expressed gene constructs. | a series of recombinant baculoviruses was constructed in order to study the influence of downstream ns2a sequences on the processing of the dengue virus ns1 glycoprotein in insect cells. ns1 alone was expressed at a high level in its native dimeric form and processed efficiently through the spodoptera frugiperda (sf) cell secretory pathway. recombinant ns1 was found associated with the plasma membrane and was also secreted into the extracellular medium. although both intra- and extracellular ns1 ... | 1995 | 9049346 |
| [an immunoenzyme assay for the detection of monoclonal antibodies against the e and ns1 proteins of the dengue virus]. | the elisa was standardized to detect monoclonal antibodies of dengue virus proteins e and ns1. one indirect elisa was applied, using c6-36 cells inoculated with the a-15 strain, isolated during the dengue 2 epidemic in 1981 as an antigen source. these cells were fixed in elisa plates at a 200,000 cell/well concentration. a cell control in similar conditions was used. specific monoclonal antibodies to both proteins were used to standardize the system. studies at different incubation periods, to d ... | 1995 | 9805082 |
| illness in journalists and relief workers involved in international humanitarian assistance efforts in somalia, 1992-93. | background: journalists and relief workers participating in international relief efforts in somalia following the intervention of outside armed forces in late 1992, were faced with a number of threats to their health. principally these threats were from endemic infectious diseases and trauma. methods: in-patient, emergency clinic, and laboratory records of u.s. military field hospitals, which provided the only available sophisticated medical care in somalia during most of the study period (decem ... | 1995 | 9815365 |
| [direct sequencing of an amplified product from a serum sample]. | it is reported the nucleotide and amino acidic sequence of a great variability region in the dengue 2 virus genome, starting from the rna of the original virus with no passage in the isolation systems. it is compared with the first strain of dengue 2 isolated during the 1981 epidemic with 4 passages in lactating mouse. results show that the nucleotide sequence of serum and of strain a15 are the same. | 1996 | 9768271 |
| production of nitrite by dengue virus-induced cytotoxic factor. | dengue type 2 virus (dv) infection induces production of a cytokine, the cytotoxic factor (cf) in the spleen of mice. the present study was undertaken to investigate the production of nitrite (no2-) by the spleen cells of mice in vitro and in vivo following inoculation of dv or cf. maximum no2- production occurred at 45 min after inoculation of 5 microg cf, both in vitro and in vivo. the no2- was produced by macrophages and t cells and not by b cells. pretreatment of cf with anti-cf antisera inh ... | 1996 | 9099923 |
| [reconstitution of hu-scid mice transplanted with human tonsillar lymphocytes immunized with dengue virus-2 in vitro and its immune response]. | to study the measure for the reconstitution of severe combined immunodeficient disease mice (scid mice) transplanted with human lymphocytes immunized with specific antigen in vitro. | 1996 | 9275474 |
| genetically engineered resistance to dengue-2 virus transmission in mosquitoes. | the control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. to reduce the ability of mosquitoes to transmit dengue viruses, a recombinant sindbis virus was used to transduce female aedes aegypti with a 567-base antisense rna targeted to the premembrane coding region of dengue type 2 (den-2) virus. the transduced mosquitoes were unable to support replication of den-2 virus in t ... | 1996 | 8629025 |
| pathogen-derived resistance to dengue type 2 virus in mosquito cells by expression of the premembrane coding region of the viral genome. | the full-length premembrane (prm) coding region of the dengue virus type 2 (den-2; jamaica) genome was expressed in c6/36 (aedes albopictus) cells in either the sense or the antisense orientation from a double subgenomic sindbis (dssin) virus. northern (rna) blot analysis confirmed the expression of sense or antisense den-2 prm rna in infected c6/36 cells. prm protein was demonstrated in cells infected with dssin virus expressing den-2 sense rnas by an immunofluorescence assay. c6/36 cells were ... | 1996 | 8642634 |
| dengue-2 virus infection of human bone marrow: characterization of dengue-2 antigen-positive stromal cells. | dengue is often associated with neutropenia and thrombocytopenia, suggesting that cells of the bone marrow may be targets of dengue viral infections. in this study we infected long-term marrow cultures with dengue type-2 (den-2) virus and characterized the viral antigen-positive cells. using immunofluorescence microscopy and immunohistochemical staining, we demonstrated two types of stromal cells that were positive for den-2 virus antigens. the first was a population of relatively small (approxi ... | 1996 | 8644906 |
| immunolocalization of the dengue virus nonstructural glycoprotein ns1 suggests a role in viral rna replication. | although many workers have investigated the maturation and processing of the flavivirus nonstructural glycoprotein ns1 in infected cells, these studies have provided little insight into a possible function for ns1. in this study we investigated the subcellular localization of ns1 both by immunofluorescence and cryo-immuno electron microscopy of infected vero and c6/36 cells. ns1 was found to be tightly associated with intracellular membranes, in particular with vesicle packets and large cytoplas ... | 1996 | 8659120 |
| langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus. | after the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, langerhans cells (lcs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. on comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly and at higher levels when the virus was injected by the i.d. route. subsequent re-challenge by the i.d. route produced an even more rapid serological response with all mice produci ... | 1996 | 8683550 |
| multiple specificities in the murine cd4+ and cd8+ t-cell response to dengue virus. | the target epitopes, serotype specificity, and cytolytic function of dengue virus-specific t cells may influence their theoretical roles in protection against secondary infection as well as the immunopathogenesis of dengue hemorrhagic fever. to study these factors in an experimental system, we isolated dengue virus-specific cd4+ and cd8+ t-cell clones from dengue-2 virus-immunized balb/c mice. the t-cell response to dengue virus in this mouse strain was heterogeneous; we identified at least five ... | 1996 | 8794288 |
| mutational analysis of a neutralization epitope on the dengue type 2 virus (den2) envelope protein: monoclonal antibody resistant den2/den4 chimeras exhibit reduced mouse neurovirulence. | the antigenic site of dengue type 2 virus (den2)-neutralizing monoclonal antibody (mab) 3h5 was investigated by mutational analysis. sequence comparisons indicated that much of the 12-amino-acid sequence extending from position 386 to 397 of the den2 envelope glycoprotein (e) previously thought to represent the den2-specific mab 3h5 binding site was also present in some dengue type 1, 3, or 4 virus strains. however, the region occupied by the glu-pro-gly sequence at upstream positions 383 to 385 ... | 1996 | 8874504 |
| dengue virus envelope glycoprotein can be secreted from insect cells as a fusion with the maltose-binding protein. | the maltose-binding protein (male) contains a signal sequence which allows its translocation in the periplasm of prokaryotic microorganisms. in this study, male was produced in spodoptera frugiperda (sf9) lepidopterian cells using the baculovirus expression system. the secretion of male, following cleavage of its signal sequence, to the supernatant fluid of recombinant baculovirus-infected sf9 cells and its affinity for maltodextrin polymers allowed recovery of significant amounts (> or = 10 mic ... | 1996 | 8882648 |
| a single nucleotide change in the e protein gene of dengue virus 2 mexican strain affects neurovirulence in mice. | eleven clones of dengue virus 2 mexican strain were selected by the size of their lytic plaques. nucleotide sequences of the clones producing large plaques (d2ml2, d2ml3 and d2ml4) revealed 11 mutations, 10 of which were silent. the substitution at nucleotide 1168 (g-->c) generates one amino acid difference at residue 390 (asp-->his) of the envelope protein (e). these clones showed high virulence in suckling mice when inoculated intracerebrally (> or = 70% mortality). however, the clones which s ... | 1996 | 8887488 |
| susceptibility of laboratory-bred rodents to the experimental infection with dengue virus type 2. | various strains of laboratory-bred rodents viz. mice [swiss, c57bl/6, c3h/hej, dba/2, balb/c, nmri (nu/nu) and bl6 (nu/nu) and their heterozygous siblings (nu/+)], mastomys natalensis, wistar rat, golden hamster and indian desert gerbil were inoculated intracerebrally (ic) with mouse-adapted dengue virus type 2 (dv-2). the inoculated animals were observed daily for dullness, anorexia, occult blood in faeces, patechial haemorrhages, lacrymation, paralysis, cachexia, death. necropsied animals were ... | 1996 | 8891094 |
| development of a purified, inactivated, dengue-2 virus vaccine prototype in vero cells: immunogenicity and protection in mice and rhesus monkeys. | the feasibility of a purified, inactivated dengue (den) vaccine made in vero cells was explored. a den-2 virus candidate was chosen for production of a monotypic, purified, inactivated vaccine (piv). virus was harvested from roller bottle culture supernatants, concentrated, and purified on sucrose gradients. the purified virus was inactivated with 0.05% formalin at 22 degrees c. after inactivation, the virus retained its antigenicity and was immunogenic in mice and rhesus monkeys, in which it el ... | 1996 | 8940206 |
| immunogenic and protective response in mice immunized with a purified, inactivated, dengue-2 virus vaccine prototype made in fetal rhesus lung cells. | the feasibility of a purified, inactivated vaccine (piv) against dengue type 2 (den-2) virus was explored. dengue-2 virus strain 16681 was used for producing a monotypic piv. virus adapted to fetal rhesus lung (frhl-2) cells was harvested from roller bottle culture supernatant fluids, concentrated, and purified on sucrose gradients. analysis of purified virus preparations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting showed primarily envelope (e) and premembra ... | 1996 | 8940981 |
| identification and characterization of receptor for dengue virus-induced macrophage cytotoxin. | the present study was undertaken to identify the receptor for dengue virus type 2 (dv) induced macrophage cytotoxin (cf2) on mouse peritoneal macrophages (mphi). the binding of 125i-labelled cf2 to mphi was saturable (15 nm), reversible, temperature, ph- and time-dependent. the saturation concentration was similar to that causing cell death. scatchard analysis showed the presence of intermediate type of affinity receptor and the number of receptor sites was 1.1 x 10(6) per cell with dissociation ... | 1996 | 8979500 |
| ultrastructural aspects of the replication of dengue virus type 2 isolated in brazil. | 1996 | 8736101 | |
| surveillance of the mosquito aedes aegypti and its biocontrol with the copepod mesocyclops aspericornis in australian wells and gold mines. | a survey of the dengue vector mosquito aedes aegypti was undertaken using funnel traps to detect immature stages (larvae and pupae) in flooded disused mine shafts and wells in charters towers, queensland, northern australia. the town has a history of dengue fever since 1885 when goldminers were the first recorded victims. during the latest dengue epidemic in 1993, 2% of the population had laboratory-confirmed dengue virus type 2, despite source reduction of ae.aegypti breeding-sites at ground le ... | 1996 | 8744708 |
| epidemic dengue 2 in the city of djibouti 1991-1992. | from october 1991 to february 1992, an outbreak of acute fever (in which thick blood films were negative for malaria) spread rapidly in the city of djibouti, djibouti republic, affecting all age groups and both nationals and foreigners. the estimated number of cases was 12,000. the clinical features were consistent with a non-haemorrhagic dengue-like illness. serum samples from 91 patients were analysed serologically for flavivirus infection (dengue 1-4, west nile, yellow fever, zika, banzi, and ... | 1996 | 8758061 |
| dengue virus-induced cytotoxin releases nitrite by spleen cells. | dengue type-2 virus (dv) infection in mice induces t cells to produce a cytokine, the cytotoxic factor (cf), which induces h2-a positive macrophages to produce another cytokine, cyototoxic (cf2), which amplifies its cytotoxic effects on target cells. the present study was undertaken to investigate the production of nitrite (no2-) by the spleen cells of mice in vitro and in vivo following inoculation of cf2. maximum no2- production occurred at 1 hour after inoculation of 100 micrograms cf2. pretr ... | 1996 | 8762862 |
| serological evidence of den-2 activity in assam and nagaland. | 1996 | 8778182 | |
| internal proteolysis of the ns3 protein specified by dengue virus 2. | the ns3 protein of flaviviruses is a multifunctional polypeptide required for virus replication. enzymic activities that have been demonstrated or predicted from the presence of sequence motifs include protease, ntpase, helicase and rna triphosphatase. both full-length and truncated forms of ns3 have been identified in infected cells. to examine internal cleavage of the ns3 protein of dengue virus 2 (den-2), infected cells or cos cells transfected with cdna encoding ns2b/3 were radiolabelled and ... | 1997 | 9018055 |
| monoclonal antibodies raised to the dengue-2 virus (cuban: a15 strain) which recognize viral structural proteins. | mouse monoclonal antibodies (mabs) were raised to dengue-2 virus (d-2v) cuban (a15) strain and the cell culture supernatants were screened by elisa using the homologous virus. three mab-secreting lines were further characterized using immunoblot, haemaglutination, complement-fixation, and neutralization assays. one of these, mab 8h8, weakly reacted with the viral nonstructural-1 protein (ns1) but more specifically identified the capsid protein (c), mab 3e1 recognized in serological assays d-2v a ... | 1997 | 9309425 |
| activity of dengue-2 virus and prevalence of aedes aegypti in the chirimiri colliery area, madhya pradesh, india. | repeated outbreaks of a suspected viral fever in chirimiri colliery area, madhya pradesh were reported since 1990. the area consists of an agglomeration of sprawling settlements at varying altitudes of 816 to 890 m and it has partial sylvan cover. during a 1992 outbreak 25 patients' sera were tested, of which 13 showed seropositivity to dengue (den) by mac-elisa test; den-2 was isolated from aedes aegypti collected from two of the eight settlements of the area. the principal vector, ae. aegypti, ... | 1997 | 9322295 |
| dengue infection in paracambi, state of rio de janeiro, 1990-1995. | a seroepidemiological survey was carried out during 1994 in the municipality of paracambi, state of rio de janeiro. haemagglutination inhibition test positivity was detected in 145 out of 370 (39.2%) schoolchildren. the frequency of positive test by sex was 53.8% (78/145) female and 46.2% (67/145) male. distribution by age showed the increasing of antibody positivity in older children. strains of dengue virus type 1 and dengue virus type 2 were isolated before (1990) showing the co-circulation o ... | 1997 | 9322424 |
| rapid characterization of genetic diversity among twelve dengue-2 virus isolates by single-strand conformation polymorphism analysis. | single-strand conformation polymorphism (sscp) analysis was used to characterize genetic polymorphisms among 12 isolates of dengue-2 virus, which were previously genetically characterized by rnase t1 oligonucleotide mapping and by sequencing the viral envelope (e) gene. specific cdna fragments from the dengue-2 isolates were amplified by the reverse transcriptase-polymerase chain reaction. the viral e, premembrane (prm), and nonstructural 5 (ns5) gene cdnas of 291 basepairs (bp), 291 bp, and 201 ... | 1997 | 9347956 |
| antibodies against dengue virus e protein peptide bind to human plasminogen and inhibit plasmin activity. | both mice and rabbits immunized with dengue virus e protein peptide spanning amino acids 100-119 (d4e) produced antibodies that reacted not only with the d4e peptide itself but also with human plasminogen, as shown by elisa and western blot. sera from dengue virus-hyperimmunized mice and dengue patients also contained antibodies against d4e and plasminogen. furthermore, such sera all contained plasmin inhibitory activity. using affinity-purified anti-d4e antibodies and free d4e peptide for compe ... | 1997 | 9353146 |
| analysis of a recombinant dengue-2 virus-dengue-3 virus hybrid envelope protein expressed in a secretory baculovirus system. | in a step towards a tetravalent dengue virus subunit vaccine which is economical to produce, highly immunogenic and stable, a hybrid dengue virus envelope (e) protein molecule has been constructed. it consists of 36 amino acids from the membrane protein, the n-terminal 288 amino acids of the dengue-2 virus e protein plus amino acids 289-424 of the dengue-3 virus e protein. it has been engineered for secretory expression by fusion to a mellitin secretory signal sequence and truncation of the hydr ... | 1997 | 9367357 |
| dengue virus infection of human t lymphocytes. | we have previously reported (kurane et al., arch. virol. 110, 91-101, 1990) that human t cell leukemia and lymphoma cell lines could be infected with dengue virus. in the present study we examined whether human t lymphocytes which are not leukemia or lymphoma cells can be infected with dengue virus. dengue virus-specific human cd4+ t cell clones, jk44 and jk49, and cd8+ t cell clones, cb2.8 and cb6.17, were infected with dengue-2 virus. dengue virus antigen-positive cells were detected in each o ... | 1997 | 9385407 |
| cotranslational membrane insertion of the serine proteinase precursor ns2b-ns3(pro) of dengue virus type 2 is required for efficient in vitro processing and is mediated through the hydrophobic regions of ns2b. | polyprotein processing of dengue virus type 2, a positive strand rna virus, is carried out by the host signal peptidase and a novel two-component viral proteinase of the serine proteinase family, ns2b/ns3(pro), in the endoplasmic reticulum. using an in vitro processing system, we examined the cis and trans cleavages of the 2b/3 and 4b/5 sites by ns2b/ns3(pro), respectively. lysates of bhk-21 cells coexpressing ns2b and ns3(pro) mediated trans cleavage of the 4b/5 site in vitro, and the protease ... | 1997 | 9388208 |
| affinity-purified dengue-2 virus envelope glycoprotein induces neutralizing antibodies and protective immunity in mice. | we constructed a recombinant baculovirus which produces a dengue (den)-2 virus envelope (e) protein containing a six-histidine (h6) tag in place of the last 100 amino acids at its c-terminus. the recombinant protein was purified from the supernatant of baculovirus-infected spodoptera frugiperda insect cell cultures to apparent homogeneity by cation-chelation chromatography (talon) in which the h6-tagged e-protein was eluted under non-denaturing conditions with 100 mm imidazole at ph 8.0. mice va ... | 1997 | 9413106 |
| origins of dengue type 2 viruses associated with increased pathogenicity in the americas. | the recent emergence and spread of dengue hemorrhagic fever in the americas have been a major source of concern. efforts to control this disease are dependent on understanding the pathogenicity of dengue viruses and their transmission dynamics. pathogenicity studies have been hampered by the lack of in vitro or in vivo models of severe dengue disease. alternatively, molecular epidemiologic studies which associate certain dengue virus genetic types with severe dengue outbreaks may point to strain ... | 1997 | 9143280 |
| construction of infectious cdna clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain pdk-53. | we identified nine nucleotide differences between the genomes of dengue-2 (den-2) 16681 virus and its vaccine derivative, strain pdk-53. these included a c-to-t (16681-to-pdk-53) mutation at nucleotide position 57 of the 5'-untranslated region, three silent mutations, and substitutions prm-29 asp to val, ns1-53 gly to asp, ns2a-181 leu to phe, ns3-250 glu to val, and ns4a-75 gly to ala. unpassaged pdk-53 vaccine contained two genetic variants as a result of partial mutation at ns3-250. we constr ... | 1997 | 9143286 |
| inoculation of plasmids expressing the dengue-2 envelope gene elicit neutralizing antibodies in mice. | to develop a nucleic acid vaccine against dengue type-2 virus, the prem and 92% of the envelope (e) genes were cloned into different eukaryotic plasmid expression vectors (pkcmvint polyli and pvr1012). the resultant plasmid constructs (pd2me and p1012d2me) properly expressed the truncated e protein in vitro as evidenced by the expected protein size on sds-page and the ability of the protein to be recognized by monoclonal antibodies directed against conformational epitopes. three-week-old balb/c ... | 1997 | 9160523 |
| risk factors in dengue shock syndrome. | despite a growing body of evidence predominantly, but not exclusively, from thailand suggesting that the risk of developing dengue shock syndrome (dss) is greatest following an anamnestic dengue infection, particularly if the most recent infection was with dengue 2 virus, there continues to be debate about the justification for these claims. this report describes a five-year, prospective study in two townships (suburbs) in yangon (rangoon) myanmar (burma) in which attempts were made to confirm t ... | 1997 | 9180609 |
| infectious rna transcripts from full-length dengue virus type 2 cdna clones made in yeast. | the dengue virus type 2 genomic rna was amplified by reverse transcription-pcr and cloned as four cdna fragments. we could not assemble these four fragments into full-length cdna in escherichia coli. the full-length dengue virus cdna was constructed by homologous recombination in yeast, either as part of a yeast artificial chromosome or in a yeast-e. coli shuttle vector. full-length cdna clones were propagated once in e. coli to prepare useful quantities of dna. in vitro transcription of these c ... | 1997 | 9188607 |
| the dengue virus nonstructural-1 protein (ns1) generates antibodies to common epitopes on human blood clotting, integrin/adhesin proteins and binds to human endothelial cells: potential implications in haemorrhagic fever pathogenesis. | antibody responses generated by mice to the dengue-2 virus ns1 protein (d-2v ns1) were influenced by mhc class ii (i-a) haplotype but each antiserum cross-reacted with human fibrinogen, thrombocytes and endothelial cells. to investigate these findings, a highly avid subclone (mab 1g5.4-a1-c3) was selected from a parent hybridoma that secreted a monoclonal antibody (mab) specific for the native dimeric form of d-2v ns1. when mab reactions were compared using a panel of overlapping synthetic pepti ... | 1997 | 9191856 |
| possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in bangkok, thailand. | cocirculation of two genetic subtypes of dengue serotype 2 viruses was first observed in the 1980 epidemic season in thailand. to further delineate the evolutionary history and the contribution of these subtypes to subsequent epidemics, we determined the envelope glycoprotein gene sequence of 20 dengue serotype 2 viruses isolated from infected patients during 1987 and compared them with those derived from earlier years. subtype iiia strains represented the majority (18 of 19) of dengue type 2 vi ... | 1997 | 9242328 |
| vertical transmission of dengue. | dengue, an important mosquito-borne flavivirus infection, is endemic in southeast asia. we describe two mothers who had acute dengue 4 and 8 days before the births of their infants. one mother had worsening of her proteinuric pregnancy-induced hypertension, liver dysfunction, and coagulopathy and required multiple transfusions of whole blood, platelets, and fresh frozen plasma. her male infant was ill at birth, developed respiratory distress and a large uncontrollable left intracerebral hemorrha ... | 1997 | 9431381 |
| global situation of dengue and dengue haemorrhagic fever, and its emergence in the americas. | about two-thirds of the world's population live in areas infested with dengue vectors, mainly aedes aegypti. all four dengue viruses are circulating, sometimes simultaneously, in most of these areas. it is estimated that up to 80 million persons become infected annually although marked underreporting results in the notification of much smaller figures. currently dengue is endemic in all continents except europe and epidemic dengue haemorrhagic fever (dhf) occurs in asia, the americas and some pa ... | 1997 | 9477544 |
| comparison of various methods of detection of different forms of dengue virus type 2 rna in cultured cells. | in this report, the sensitivity of various methods of detection of dengue virus type 2 (den-2) sense, antisense, replicative intermediate (ri) and replicative form (rf) rnas in infected mosquito aedes pseudoscutellaris ap-61 and mammalian baby hamster kidney bhk-21 cells is compared. licl precipitation was used for separation of viral rf rna from ri rna. our results show that reverse transcription-polymerase chain reaction (rt-pcr) followed by southern blot analysis and slot blot hybridisation o ... | 1997 | 9607089 |
| effect of sublethal dosages of malathion on the oral susceptibility of aedes aegypti to dengue-2 virus infection. | 1997 | 9561626 | |
| antibody responses of dengue fever patients to dengue 2 (new guinea c strain) viral proteins. | the present study was undertaken to investigate the antibody responses of dengue fever (df) patients to specific dengue virus proteins. partially purified dengue 2 new guinea c (ngc) strain virus was used as antigen. under the present experimental protocols, it was observed that almost all df patients' sera had detectable presence of antibodies which recognize the dengue 2 envelope (e) protein. the convalescent-phase sera especially had significant detectable igg, igm and ige against the protein ... | 1997 | 10879241 |
| [cloning of the ns1 gene of dengue-2 virus and determination of its partial nucleotide sequence]. | in this study, rt-pcr product of the ns1 gene of dengue-2 virus isolated in china was inserted into the dna of t-vector plasmid. dnas of the positive clones, which were identified by using the blue/white spot and pcr, were digested with enzymes, indicating that the inserted fragments were the same sizes as those of the amplified ns1 genes. the result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified ns1 fragm ... | 1997 | 15619908 |
| cellular proteins bind to the 3' and 5' untranslated regions of dengue 2 virus genome. | in vitro generated cloned full length dengue 2 virus untranslated regions (utrs) were used in rna gel mobility shift assays to examine cellular factors binding to the virus genomes. cellular factors in lysates of vero (monkey) and c6/36 (mosquito) cells bound specifically and non-specifically to the dengue 2 virus 3' utr. non-specific interaction with the 5' utr, resulting in formation of at least 4 band shift complexes was noted with lysate of the c6/36 cells only. pre-treating the cell lysates ... | 1998 | 10879258 |
| cardiac involvement in dengue haemorrhagic fever. | we studied 17 consecutive patients of dengue haemorrhagic fever/dengue shock syndrome (dhf/dss) to assess cardiac function by radionuclide ventriculography, echocardiography and electrocardiography (ecg) during the epidemic of dengue virus type-2 (den-2) in delhi, india (1996). case definitions laid down by the who were followed. fourteen patients were seropositive for dengue infection. in radionuclide ventriculography study, the mean left-ventricular ejection fraction was 41.69 (5.04% (range 33 ... | 1998 | 9579814 |
| evaluation of the protective efficacy of a recombinant dengue envelope b domain fusion protein against dengue 2 virus infection in mice. | a recombinant protein containing part of the dengue (den) 2 envelope protein was evaluated as a subunit immunogen for vaccination against den virus infection. a gene fragment encoding amino acids 298-400 (b domain) of the den-2 virus envelope was expressed as a fusion protein with the maltose binding protein (mbp) of escherichia coli. this recombinant, den-2(b)/mbp, was purified and analyzed for its antigenicity, immunogenicity, and ability to protect mice against lethal challenge. the recombina ... | 1998 | 9598457 |
| molecular and in vitro analysis of eight dengue type 2 viruses isolated from patients exhibiting different disease severities. | potential genetic determinants of dengue virulence were studied by sequencing the entire genomes of eight dengue 2 virus strains isolated from patients exhibiting different disease severities during an epidemic season in northeastern thailand in 1993. the isolates came from one dengue shock syndrome (thnh-7/93), three dengue hemorrhagic fever, and four dengue fever patients. phylogenetic analysis showed that the isolates belonged to the southeast asian genotype. the 3' noncoding regions showed d ... | 1998 | 9601513 |
| molecular epidemiology of malaysian dengue 2 viruses isolated over twenty-five years (1968-1993). | the limited sequencing approach was used to study the molecular epidemiology of 24 malaysian dengue 2 viruses which were isolated between 1968 and 1993. the sequences of a 240-nucleotide-long region across the envelope/non-structural 1 protein (e/ns1) gene junction of the isolates were determined and analysed. alignment and comparison of the nucleotide and deduced amino acid sequences of the isolates revealed that nucleotide changes occurred mostly at the third position of a particular codon and ... | 1998 | 9923022 |
| [epidemic outbreak of dengue virus 2/jamaica genotype in bolivia]. | to confirm an epidemic outbreak of dengue virus in the city of santa cruz, bolivia, and to determine the serotype of the virus, to estimate the rate of attack and the proportion of symptomatic infections. | 1998 | 9927881 |