Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a rapid quantitative agar gel precipitin test. | 1983 | 6306011 | |
| infectious bursal disease in western australia. | 1983 | 6307244 | |
| use of inactivated oil emulsion infectious bursal disease vaccines in breeder chickens to prevent immunosuppression in progeny chicks. | two chicken flocks, vaccinated with different inactivated infectious bursal disease vaccines, and one unvaccinated flock provided chicks with high and low levels of and no maternally derived immunity. following challenge at three ages with a subclinical strain of infectious bursal disease virus the chicks were assessed for bursal damage and suppression of the immune response to newcastle disease virus. both high and low levels of maternally derived antibody prevented immunosuppression but the lo ... | 1983 | 6312519 |
| effects of dual infection of chickens with mycoplasma synoviae and mycoplasma gallinaceum or infectious bursal disease virus on infectious synovitis. | 1983 | 6314027 | |
| the molecular biology of infectious pancreatic necrosis virus: a review. | 1983 | 6303539 | |
| [thermostability of the vaccination strain of infectious bursal disease virus]. | the vaccination strain of infectious bursal disease virus, multiplied in cultures of chick embryo cells, was very resistant to heat. at a temperature of 56 degrees c the infection titre of the virus (tcid50) decreased by 0.9 log10 within two hours and by 1.2 log10 within five hours, but the virus remained infective still after 24 hours. at a temperature of 37 degrees c, a slight decrease in infection titre was recorded only after two days and a decrease by 1.2 log10 was recorded within ten days. ... | 1983 | 6302969 |
| observations on the pathogenicity of alcaligenes faecalis in chickens. | a series of trials was conducted in which specific-pathogen-free (spf) leghorn chicks were exposed to various isolates of alcaligenes faecalis. chicks were inoculated with a. faecalis alone or in combination with newcastle disease/infectious bronchitis (nc/br) vaccine, laryngotracheitis vaccine, infectious bursal disease virus, or mycoplasma gallisepticum. the response was evaluated by morbidity, mortality, airsacculitis, reisolation of a. faecalis, and histopathological lesions of tracheas. alt ... | 1983 | 6227315 |
| effects of avian viruses on cultured chicken bone-marrow-derived macrophages. | cultured chicken bone-marrow-derived macrophages have been assayed for their susceptibility to infection with various avian viruses. three criteria of infection were employed: (1) virus-induced alterations in cell morphology ; (2) presence of intracellular viral antigens detectable by immunofluorescence; (3) kinetics of virus release by infected macrophages. macrophages proved to be resistant to marek's disease virus (mdv), herpesvirus of turkeys (hvt-fc126), infectious bronchitis virus (ibv) an ... | 1983 | 18766776 |
| serological survey of wild birds in australia for the prevalence of antibodies to egg drop syndrome 1976 (eds-76) and infectious bursal disease viruses. | serum samples from 11 species of seasonal or sedentary wild water birds in western australia were examined for antibodies to viruses endemic in commercial chickens in australia. antibodies to two serotypes of infectious bursal disease virus, and to egg drop syndrome 1976 (eds-76) virus were demonstrated in these sera. | 1983 | 18766769 |
| growth inhibition of marek's disease t-lymphoblastoid cell lines by chicken bone-marrow-derived macrophages activated in vitro. | studies have been performed on the induction of cytostatic activity of cultured chicken bone-marrow-derived macrophages. cultured macrophages were exposed to supernatants of cona-stimulated spleen cell cultures (lymphokines), lipopolysaccharide (lps), cona or infectious bursal disease virus (ibdv). cytostatic activity of macrophages was examined by testing their growth-inhibiting effect on t-lymphoblastoid marek's disease cell lines rp-1, hp-2 and msb-1 as target cells. lymphokines, lps and ibdv ... | 1983 | 18766775 |
| activating effects of interferons, lymphokines and viruses on cultured chicken macrophages. | cultured chicken bone-marrow-derived and blood monocyte-derived macrophages could be activated by various treatments: (1) with crude lymphokine preparations obtained from concanavalin a (cona)-stimulated chicken spleen or thymus cell cultures: (2) with virus-induced interferons (ifn) from cultured chicken embryo fibroblasts or macrophages; (3) with inactivated reovirus or live infectious bursal disease virus (ibdv). macrophage activity was expressed by cytostatic effects against lymphoblastoid t ... | 1984 | 18766874 |
| concurrence of nephrosis-nephritis due to infectious bronchitis virus and infectious bursal disease in broiler chickens. | a condition showing nephrosis-nephritis caused by infectious bronchitis virus (ibv) and atrophy of the bursa of fabricius attributable to infectious bursal disease virus (ibdv) occurred with a mortality rate of 6.4%, 7.4% and 8.5% in young broiler chickens in three flocks on a poultry farm. eighteen birds from the outbreaks were studied histologically and ultra-structurally. nephrosis-nephritis appeared initially as degenerative changes of the convoluted tubules, followed by inflammatory reactio ... | 1984 | 18766836 |
| experimental infection of chickens with an adenovirus isolated from tenosynovitis, following infection with either (1) an arthrotropic reovirus, or (2) infectious bursal disease virus. | in an attempt to show arthrotropic activity for an adenovirus isolated from tenosynovitis in chickens, but which did not cause tenosynovitis on its own, two dual infections were conducted. in the first, specific pathogen-free (spf) chicks were infected with a tenosynovitis-inducing reovirus at 1-day-old, and the adenovirus was given 3 days later. in both cases viruses were administered orally or via the footpad. the adenovirus did not exacerbate reovirus-induced lesions of tenosynovitis. there w ... | 1984 | 18766845 |
| the effect of antigen variation on the quantitative agar gel precipitin test for antibodies to infectious bursal disease virus. | 1984 | 6207175 | |
| failure of two serotype ii infectious bursal disease viruses to affect the humoral immune response of turkeys. | the effect of serotype ii infectious bursal disease virus (ibdv) isolates from turkeys on the homoral immune response of turkey poults was determined. following exposure to the oh ibdv isolate, poults in two experiments were inoculated with sheep red blood cells, which is a t-dependent antigen, and poults in two other experiments were inoculated with salmonella heidelberg o antigen, which is a t-independent antigen. prior exposure to serotype ii ibdv did not affect serum antibody titers to these ... | 1984 | 6202292 |
| expression of viral arthritis-tenosynovitis infection in immunosuppressed chickens. | previously untreated chicks and those treated neonatally with either cyclophosphamide (cy) or infectious bursal disease virus (ibdv) were subcutaneously inoculated with viral arthritis virus ( vav ) to determine whether suppression of humoral immunity would affect vav pathogenicity. no difference was apparent in the disease process that occurred in the immunosuppressed versus the nonimmunosuppressed chickens. however, in the immunosuppressed birds, vav was recovered from the heart and tendon tis ... | 1984 | 6203105 |
| age resistance in chickens against infectious bursal disease. | 1984 | 6099968 | |
| nucleic acid and structural proteins of infectious bursal disease virus isolates belonging to serotypes i and ii. | the nucleic acid and structural proteins of infectious bursal disease virus serotype i (six isolates) were compared with those of serotype ii (two isolates). five of the serotype i isolates originated from chickens, whereas both serotype ii isolates were from turkeys. the growth curves of representative isolates of both serotypes were similar, but the latent period and virus yield were different. the seven isolates tested had two segments of double-stranded genomic rna. the rna migration pattern ... | 1984 | 6098258 |
| serological survey of infectious bursal disease virus serotypes 1 and 2 in california turkeys. | the prevalence of two infectious bursal disease (ibd) viruses--serotype 1, of chicken origin, and serotype 2, of turkey origin--was studied in california turkeys. serum samples were collected from 15 turkey flocks representing nine counties. the virus-neutralization test was used. overall, only 15% of 342 samples were positive for serotype 1, whereas 89% were positive for serotype 2. however, 12 out of 15 flocks had a least one sample positive for serotype 1, and 14 out of 15 flocks had at least ... | 1984 | 6098247 |
| interference between human hepatitis a virus and an attenuated apathogenic avian virus. | the effect of an attenuated apathogenic avian bursa virus on the course of human hepatitis a viral infection was studied in marmoset monkeys. the monkeys were infected with human hepatitis a virus, then superinfected with avian bursa virus one and three weeks after initial inoculation with human hepatitis a virus. the superinfected monkeys did not show the characteristic serum glutamic pyruvic transaminase (sgpt) elevation. also their liver biopsies showed no pathologic changes. the virus contro ... | 1984 | 6087596 |
| rapid serological profiling by enzyme-linked immunosorbent assay. iii. simultaneous measurements of antibody titers to infectious bronchitis, infectious bursal disease, and newcastle disease viruses in a single serum dilution. | the present paper describes a method for measuring enzyme-linked immunosorbent assay (elisa) antibody titers to infectious bronchitis (ib), infectious bursal disease (ibd), and newcastle disease (nd) viruses from a single serum dilution using the same assay procedures and analyses. a regression line equation generated for predicting ndv elisa antibody titers at a single serum dilution was successfully used to predict elisa antibody titers to ib and ibd antigen preparations at the same test sera ... | 1984 | 6326733 |
| further studies on the immunization of chickens with temperature-sensitive mycoplasma gallisepticum mutant. | newly hatched chickens were immunized with a temperature-sensitive (ts) mycoplasma gallisepticum (mg) mutant (ts 100). immunized chickens resisted challenge with the virulent s6 strain. the dose of ts mg needed for protection was less than 3.3 x 10(4) colony-forming units. after immunization with ts 100, chickens were subjected to a variety of virus infection and immunosuppressive treatments. neonatal bursectomy or thymectomy, infectious bursal disease virus infection, and infectious bronchitis ... | 1984 | 6326734 |
| the effect of oil-emulsion vaccines on the occurrence of nonspecific plate agglutination reactions for mycoplasma gallisepticum and m. synoviae. | six groups of ten 18-week-old mycoplasma-free white leghorn pullets were vaccinated with one of the following: mycoplasma gallisepticum (mg) bacterin. haemophilus gallinarum bacterin, pasteurella multocida bacterin, combined infectious bursal disease (ibd)-newcastle (ndv) chicken-embryo-origin (ceo) vaccine. ibd-ndv tissue-culture-origin (tc) vaccine, or saline emulsified in oil; one group received no vaccine. plate agglutination tests for m. synoviae (ms) and mg were done for 10 weeks after vac ... | 1984 | 6331364 |
| [asymptomatic course of infectious bursitis in chickens]. | morphologic and serologic studies were carried out with a total of 190 birds of three breeds (cornish, white plymouth rock, and gillink) aged 1 to 50 days, with which gumboro disease ran a symptomless course. gross lesions were established in 3.1 per cent of the birds only. histopathologically, however, there were changes in the bursa fabricii (73.6 per cent), the spleen (68.4 per cent), the thymus (10.5 per cent), and the cecal tonsils (5.2 per cent). the microscopic lesions consisted in malaci ... | 1984 | 6099626 |
| a survey of disease in five commercial flocks of meat breeder chickens. | causes of sickness and death in approximately 30,000 chickens in 5 meat breeder flocks were investigated between may 1979 and april 1980. approximately 23% of disease was due to neoplasms; 81% of these were marek's disease despite vaccination against this infection. other frequent diagnoses included cellulitis (15%), respiratory disease (14%), lesions of the reproductive tract (11%) and tenosynovitis/arthritis (9%). antibodies to mycoplasma gallisepticium, avian adenovirus, infectious bursal dis ... | 1984 | 6704066 |
| experimental infection of turkeys with infectious bursal disease virus and the effect on the immunocompetence of infected turkeys. | turkeys of various ages were infected with strains of infectious bursal disease virus (ibdv). pathological signs of infection were detected only in turkeys 1 day old when infected. no differences in antibody responses to ibdv or virus recovery were observed between turkeys of various ages at the time of infection. cellular and humoral immune responses were suppressed following infection of day-old turkeys. the effects varied with virus strain. | 1984 | 6326741 |
| reaction patterns of five strains of infectious pancreatic necrosis virus in enzyme-linked immunosorbent assay. | 1984 | 6084094 | |
| [pathomorphological studies of infectious bursitis in chickens with a subclinical infection]. | a total of 70 growing birds of three breed, aged 1 to 42 days in which the disease was serologically and morphologically demonstrated were studied. no apparent gross lesions were established with any of the birds. histologically, there was in the bursa of fabricius a drop of the lymphocytes within the lymphoreticular system of the follicles. there were in the center of the follicles histiocytes, and epithelial proliferation was seen with single follicles. the cover epithelium of the bursa presen ... | 1984 | 6089408 |
| vaccination against marek's disease and infectious bursal disease. ii. titration and in vivo efficacy of the coinfection-derived bivalent live vaccine for turkey herpesvirus and infectious bursal disease virus. | the bivalent hvt/ibdv live vaccine, developed by cocultivation of the turkey herpesvirus (hvt) and infectious bursal disease virus (ibdv) vaccine in cell cultures, was quantitated. the titer of each constituent virus could be successfully determined in vitro by pretreatment of the vaccine with anti-ibdv serum or chloroform (chcl3). studies in vivo further demonstrated that the anti-ibdv serum-treated hvt/ibdv vaccine lost its immunogenicity by failing to induce ibdv-neutralizing antibody or to p ... | 1984 | 6091083 |
| morphological changes in blood lymphocytes of chickens infected with infectious bursal disease virus. | 1984 | 6091373 | |
| effect of infectious bursal disease virus on protection against marek's disease by turkey herpesvirus vaccine. | chickens inoculated at hatch with pathogenic infectious bursal disease virus (ibdv) and the turkey herpesvirus (hvt) vaccine were poorly protected against challenge at 1 week with virulent marek's disease virus (mdv). however, if vaccination with hvt preceded ibdv inoculation such as by embryonal vaccination with hvt and exposure to ibdv at hatch, or by vaccination at hatch and ibdv exposure 5 days or longer postvaccination, then the vaccinal efficacy of hvt was not influenced. further, the inhi ... | 1984 | 6091603 |
| serologic evidence of infectious bursal disease virus serotype ii infection in minnesota turkeys. | serum samples from seven randomly selected minnesota turkey flocks were tested for antibodies to infectious bursal disease virus serotype i (lukert strain, isolated from chickens, and north carolina strain, isolated from turkeys) using a virus-neutralization (vn) test. all flocks were found to have low antibody titers to both lukert and north carolina strains. five out of the seven flocks had high vn titers to the missouri strain, a serotype ii virus isolated from turkeys. | 1984 | 6091608 |
| capsid symmetry of viruses of the proposed birnavirus group. | the capsid architecture of three members of the tentatively formed group of bisegmented ds rna containing animal viruses (birnavirus group) was studied in comparison with the fine structure of reovirus type 1 using high resolution negative staining and shadowing techniques followed by rotational enhancement analysis. ipnv of the trout, ibdv of the chicken, and eel virus (berlin) are single-shelled particles with a capsid diameter of 61-65 nm and thus clearly distinguishable from the double-shell ... | 1985 | 3994515 |
| the use in practice of inactivated oil emulsion vaccine against infectious bursal disease in broiler breeders and its influence on the progeny: a comparative field trial. | field trials were conducted to establish the effect of the use of an inactivated oil emulsion vaccine against infectious bursal disease (ibd oev) in broiler breeder hens, and its effects on their progeny. the performance of 18 broiler flocks, which were the progeny of the ibd oev vaccinated breeder hens, but which were not vaccinated with a live vaccine against ibd, was equal to that of broiler flocks which were vaccinated with a live ibd vaccine and originated from parent stock that had been va ... | 1985 | 2990089 |
| antibody to the 32k structural protein of infectious bursal disease virus neutralizes viral infectivity in vitro and confers protection on young chickens. | chicken sera containing igg antibodies specific for the 32 000 (32k) mol. wt. structural polypeptide of infectious bursal disease (ibd) virus, as assessed by western blotting, neutralized the in vitro infectivity of tissue culture-adapted ibd virus. when injected into young chickens, the serum passively protected them from challenge with pathogenic ibd virus. chickens immunized with the 32k structural polypeptide of ibd virus, prepared by electroelution from sds-page gels, produced antibody dete ... | 1985 | 2999312 |
| embryo vaccination with infectious bursal disease virus alone or in combination with marek's disease vaccine. | studies with specific-pathogen-free chickens revealed that chicks hatching from eggs inoculated at the 18th day of embryonation with infectious bursal disease (ibd) vaccine viruses of low virulence (isolates tc-ibdv and bvm-ibdv) developed antibody against ibd virus (ibdv) and resisted challenge with virulent ibdv at 3 weeks of age or older. embryo vaccination did not adversely affect hatchability of chicks or survival of hatched chicks. chicks embryonally vaccinated with tc-ibdv had transient h ... | 1985 | 3008698 |
| [electron microscopic research on an experimental infection in chicks caused by the infectious bursal virus]. | a total of 18 birds at the age of 25 days were infected intraocularly with the pathogenic strain 52/70 of the infectious bursitis virus. the bursa of fabricius and the thymus were sampled for electron-microscopic investigations. the ultrastructural changes in the infected cells consisted in pycnosis of the lymphocyte and macrophage nuclei, vacuolization of the cytoplasm, and the production of lipid droplets as well as of inclusion bodies of varying density, myelin figures, multivericular bodies, ... | 1985 | 3002003 |
| immunogenicity and antigenicity of infectious bursal disease virus serotypes i and ii in chickens. | chickens were inoculated with infectious bursal disease virus serotype i or serotype ii to determine if their immune system can distinguish between the two serotypes. chickens had neutralizing antibodies to only serotype i viruses following exposure to serotype i viruses, and chickens had antibodies to only serotype ii viruses following exposure to serotype ii viruses. no cross-reactions were observed between antisera prepared to each of these two serotypes using a cross-virus-neutralization ass ... | 1985 | 3008699 |
| counter immunoelectroosmophoresis in the diagnosis of infectious bursal disease of poultry. | infectious bursal disease virus antigen was detected in the bursa of fabricius of infected birds by the use of the counter immunoelectroosmophoresis technique. eight suspected outbreaks were confirmed in this way and 82 out of 89 bursal samples submitted for laboratory confirmation were positive. the test was also suitable for the detection of antibody to infectious bursal disease virus in sera of infected birds. precipitin lines were visible within 30 min as compared with 18 to 24 h with the ou ... | 1985 | 3003985 |
| vaccination against marek's disease and infectious bursal disease iii. growth rate of both turkey herpesvirus and infectious bursal disease virus in coinfected cell cultures. | titers of the turkey herpesvirus (hvt) and infectious bursal disease virus (ibdv) in coinfected cell cultures, used to produce a live bivalent hvt/ibdv vaccine, were determined daily. based on the daily titers, cells harvested either 2 or 3 days after inoculating the second virus (ibdv) into hvt infected cell cultures yielded the maximum titers for both viruses. harvesting coinfected cell cultures on either day provided the most suitable source of the live hvt/ibdv vaccine against marek's diseas ... | 1985 | 2987896 |
| detection of antibodies against infectious bursal disease virus: a comparison of three serological methods. | four different oil emulsion infectious bursal disease virus (ibdv) vaccines were inoculated into four-week-old specific pathogen-free chickens. at weekly intervals for five weeks, sera were obtained from the vaccinated birds and from uninoculated control birds and examined for antibodies against ibdv by enzyme-linked immunosorbent assay (elisa), the quantitative agar gel precipitin (qagp) test and the virus neutralisation (vn) test. there was a highly significant correlation between the mean res ... | 1985 | 2988091 |
| an enzyme-linked immunosorbent assay method for the simultaneous measurement of antibody titer to multiple viral, bacterial or protein antigens. | a model enzyme-linked immunosorbent assay (elisa) system was developed which permits the user to evaluate replicate single sera dilutions of 46 test and control serum samples for the presence of specific antibody against up to eight different antigens in one assay. the system makes use of a transplanting device for the simultaneous transfer of aliquots of independently diluted replicate test samples along with conjugate and substrate from a serum reservoir plate or from a reagent reservoir onto ... | 1985 | 3004010 |
| serological survey of the incidence of infectious bursal disease serotypes 1 and 2 in chicken and turkey flocks in england. | 1985 | 3000053 | |
| comparison of a kinetic-based enzyme-linked immunosorbent assay (kelisa) and virus-neutralization test for infectious bursal disease virus. i. quantitation of antibody in white leghorn hens. | the kinetics of the enzyme-substrate reaction served to evaluate a single-serum-dilution indirect enzyme-linked immunosorbent assay (elisa) for quantitating antibody in white leghorn hens inoculated with infectious bursal disease virus (ibdv) vaccines. the antibody profiles of primary and secondary responses induced by two ibdv immunization regimens were compared using a kinetic-based elisa (kelisa) and the virus-neutralization (vn) test. kelisa was standardized with an ibdv-infected vero cell s ... | 1985 | 3000335 |
| [associated vaccination of poultry against infectious bronchitis, newcastle disease and infectious bursitis]. | the effectiveness of immunity was studied following a mixed vaccination with live vaccines against infectious bronchitis (strains h120 and h52), newcastle disease (strain la sota), and infectious bursitis (strain th75vn82). the three vaccines were applied simultaneously via the drinking water, through the spray method, and nasally. experiments were carried out with a total of 31,466 birds: broilers, growing layers, broiler parents--all without preliminary treatment with biopreparations. immunity ... | 1985 | 3002010 |
| effect of avian reoviruses on lymphoid organ weights and antibody response in chickens. | several avian reoviruses were screened to determine their effects on the immune system by inoculating them subcutaneously (sq) into day-old chicks. for comparison, infectious bursal disease virus (ibdv) was similarly evaluated. the response of the immune system was measured functionally by the hemagglutination-inhibition (hi) response to newcastle disease virus (ndv) and structurally by changes in the organ-to-body-weight ratios of the bursa of fabricius, thymus, and spleen. when inoculated sq, ... | 1985 | 2992443 |
| the characterization and molecular cloning of the double-stranded rna genome of an australian strain of infectious bursal disease virus. | the genome of infectious bursal disease virus (ibdv) strain 002-73 was found to consist of two segments of double-stranded (ds) rna which were 3400 bp (mw 2.06 x 10(6)) and 2900 bp (mw 1.76 x 10(6)) long, respectively. the ds ibdv rna could be translated, in vitro, only after extensive denaturation. the small rna segment was found to code for a single polypeptide of mw 90k, while the large rna segment coded for three major polypeptides of mw 52k, 32k, and 28k, and two minor polypeptides of mw 41 ... | 1985 | 2998012 |
| increased virulence of an infectious bursal disease live virus vaccine after passage in chicks. | a live infectious bursal disease virus vaccine increased in virulence when passaged six times in susceptible birds, as judged by damage to the bursa of fabricius. it is suggested that the increase in virulence of this vaccine was due to the selection of a virulent subpopulation which had been present in the vaccine since its original isolation from the field and which had not been eliminated during its attenuation by passage in cell culture. the vaccine was then plaque purified and a virus strai ... | 1985 | 2998114 |
| characterization by western blotting of the immunogens of infectious bursal disease virus. | the australian isolate of infectious bursal disease (ibd) virus (002/73) was purified from infected bursae by rate-zonal and density-equilibrium centrifugation and characterized by polyacrylamide gel electrophoresis. two major polypeptides having approximate mol. wt. of 32 000 (32k) and 37k and three other polypeptides of approximate mol. wt. 29k, 41.5k and 91.5k were present in all preparations of virus having a buoyant density of 1.33 g/ml. western blotting of the polypeptides of ibd virus sho ... | 1985 | 2410555 |
| simultaneous application of live turkey herpesvirus and infectious bursal disease vaccines against marek's disease and infectious bursal disease. | the efficacy of cell-associated (ca) and cell-free (cf) infectious bursal disease (ibd) vaccines in maternal antibody (mab)-bearing broiler chickens was compared. compatibility and potency of a mixed vaccine combining live ca turkey herpesvirus (hvt) and ca ibd vaccine viruses were also tested against gross lesions induced by marek's disease (md) or ibd in specific-pathogen-free chickens. both ca and cf ibd vaccines provided significant protection against gross bursal lesions of ibd in the prese ... | 1985 | 2983300 |
| effects of chemically or virus-induced immunodepression on response of chickens to avian leukosis virus. | the effects of chemically or virus-induced immunodepression on the infection profile (development of viremia and antibody) and shedding of avian leukosis virus (alv) were studied in progeny chickens of experimental or commercial breeder flocks. chickens were infected with alv subgroup a by contact at hatching and by oral inoculation at 4-5 weeks of age. in the first experiment, chickens were inoculated with a virulent strain of infectious bursal disease virus (ibdv) at 1 day or 6 weeks of age. i ... | 1985 | 2985037 |
| antibody responses and virus reisolation in turkeys experimentally infected with an infectious bursal disease isolate. | following the infection of turkey poults with a field isolate of infectious bursal disease virus, antibody levels were examined and reisolation of the virus was attempted. after inoculation at 36 days of age, peak titres in both the inoculated and a contact-exposed group were obtained after 13 days. the titres fell slightly during the next week and then remained level until the experiment was terminated at 91 days of age. virus was reisolated from faeces from day 3 until day 8 after inoculation ... | 1985 | 2986338 |
| the isolation of infectious bursal disease virus from turkeys in england. | 1985 | 2986755 | |
| comparison of virus neutralizing and precipitating antibodies to infectious bursal disease virus and their effect on susceptibility to challenge. | 1985 | 2986756 | |
| in vitro and in vivo characterization of avian escherichia coli. ii. factors associated with pathogenicity. | the pathogenicity of 197 escherichia coli isolates obtained from clinically affected commercially grown broiler chickens and normal hatchery chicks was assessed by inoculating day-old broilers intratracheally. the degree of pathogenicity (high, intermediate, low) was judged according to mortality and lesions occurring within 7 days following inoculation. serotype, metabolic activity, motility, and in vitro antibiotic sensitivity of each isolate were evaluated and related to pathogenicity. sevent ... | 1985 | 3914272 |
| studies on the possible roles of naturally infected nigerian local chickens and vaccine virus in the epidemiology of infectious bursal disease. | 1985 | 3022357 | |
| histopathology of infectious bursal disease in non-lymphoid organs of chickens. | 1985 | 3020648 | |
| absence of influence on immune competence by the sex-linked gene (k) determining slow feathering in white leghorn chickens. | males of white leghorn strain crosses heterozygous (kk) for the sex-linked feathering locus genes were mated to rapid-feathering (k-) females to produce rapid- and slow-feathering chicks of both sexes. k did not influence humoral-mediated immunity against challenge with sheep erythrocytes, killed brucella abortus, or killed infectious bursal disease virus. chicks challenged at 3 weeks of age had higher primary responses and higher titers of 2-mercaptoethanol-resistant antibody (igg) than those c ... | 1986 | 3814012 |
| human effects of veterinary biological products. | a 14-year-old male drank two glasses of milk from a gallon inoculated with 21 vials of live virus vaccine intended to immunize 1000 baby chicks against newcastle disease. the patient was managed with catharsis and careful observation, and remained completely asymptomatic in the following 28 days. newcastle disease virus (ndv) may cause paralytic neurotropic, viscerotropic, or pneumonotropic disease in chickens, but has limited effects on humans. systemic exposure to this vaccine has not previous ... | 1986 | 3024384 |
| [a hemorrhagic-anemic syndrome with dermatitis in broiler chickens]. | eleven successive deaths of broiler chickens affected with dermatitis (staphylococcus aureus) preceded by anaemia and muscular haemorrhages, are reported as occurring in belgium. all of these cases could be traced to a single flock of broiler breeders. the depletion of lymphoid cells in the bursa, thymus and spleen was a striking feature. infectious bursal disease virus as triggering or causative agent, could not be demonstrated. the pathological changes showed a marked resemblance to similar sy ... | 1986 | 3738883 |
| replication of infectious bursal disease virus in lymphoid cells. | replication of infectious bursal disease virus in lymphoid cells from the bursa of fabricius occurs preferentially in a population of proliferating cells characterized by increased thymidine incorporation and by blast cell formation. this was demonstrated by enrichment of virus producing cells in gradients of bovine serum albumin and by mitogenic stimulation of cells with fetal calf serum or an extract prepared from bursae of susceptible chickens. susceptibility of lymphoid bursa cells is not co ... | 1986 | 3004385 |
| effect of attenuated viral vaccines on suckling mice infected with lcmv. | a single intraperitoneal treatment with live newcastle disease virus (ndv) containing attenuated ndv vaccine, and with live infectious bursal disease virus (ibdv) containing attenuated ibdv vaccine, one day before intracerebral infection with lymphocytic choriomeningitis virus (lcmv) increased, whereas a similar treatment with inactivated ndv or ibdv vaccine did not influence the death rate of suckling mice from experimental lymphocytic choriomeningitis. thus the attenuated live vaccine stimulat ... | 1986 | 2820179 |
| effect of maternal antibody on timing of initial vaccination of young white leghorn chickens against infectious bursal disease virus. | maternal antibody titers in white leghorn chicks against infectious bursal disease virus (ibdv) were measured by a computer-assisted, single-serum-dilution, indirect kinetic-based enzyme-linked immunosorbent assay (kelisa) and by a virus-neutralization (vn) test in order to predict the timing of initial vaccination. day-old white leghorns were from unvaccinated pullets or from pullets vaccinated either four times or twice with ibdv commercial vaccines. the chicks were immunized once via the drin ... | 1986 | 3028351 |
| histopathologic changes induced by serotype ii infectious bursal disease virus in specific-pathogen-free chickens. | specific-pathogen-free (spf) chickens were infected with infectious bursal disease virus (ibdv) serotype ii (turkey, missouri isolate) at 1 day or 4 weeks of age. chickens infected at 1 day had small bursas 3 weeks postinfection (pi). bursas were necrotic and inflamed through 1 week pi. post-necrotic atrophy of the bursas was marked at 3 weeks pi, and plasma cells in the harderian gland were depleted. chickens infected at 4 weeks had slightly enlarged spleens with small gray foci on the surface ... | 1986 | 3028355 |
| embryo vaccination of specific-pathogen-free chickens with infectious bursal disease virus: tissue distribution of the vaccine virus and protection of hatched chickens against disease. | vaccination of specific-pathogen-free chickens as 18-day embryos with the bvm isolate of infectious bursal disease virus (ibdv) resulted in extensive replication of the vaccine virus in the embryonic tissues. the virus was recovered from lung, thymus, proventriculus, liver, kidney, and spleen of embryos 1 day postvaccination, and recoverable virus persisted for at least 7 days. replication and spread of the vaccine virus in chickens vaccinated as 18-day embryos was compared with that in chickens ... | 1986 | 3028357 |
| mechanism of suppression by bovine serum on growth of infectious bursal disease virus. | 1986 | 3029491 | |
| expression in escherichia coli of cdna fragments encoding the gene for the host-protective antigen of infectious bursal disease virus. | the larger segment of the ibdv genome codes for a 32-kda host-protective antigen. inserts from a cdna library in pbr 322, containing overlapping cdna fragments of varying sizes and covering the entire large segment of the ibdv genome, were subcloned into a mixture of expression vectors pur 290, 291, and 292. clones expressing the host-protective antigen, or parts of it, were identified by an immunoblot assay and the fusion proteins were further characterized by western blot analysis using a mono ... | 1986 | 3004025 |
| genomic structure of the large rna segment of infectious bursal disease virus. | the larger rna segment of infectious bursal disease virus (ibdv: australian strain 002-73) has been characterized by cdna cloning and nucleotide sequence analysis. we believe ibdv is the first birnavirus to be sequenced and so have confirmed the coding region by n-terminal amino acid sequence analysis of intact viral proteins and several tryptic peptide fragments. the large rna segment encodes in order the 37-kda, 28-kda and 32-kda proteins within a continuous open reading frame and the primary ... | 1986 | 3014441 |
| [enhanced pathenogicity of chicken anemia agent (caa) in dual infections with marek's disease virus (mdv), infectious bursal disease virus (ibdv) or reticuloendotheliosis virus (rev)]. | 1986 | 3014789 | |
| rapid serological profiling by enzyme-linked immunosorbent assay. iv. association of infectious bursal disease serology with broiler flock performance. | breeder and broiler flocks were serologically evaluated using a multiple enzyme-linked immunosorbent assay (m-elisa). the serologic status of two commercial broiler-breeder flocks and their progeny was monitored, and 840 sera were promptly assessed for antibodies against six infectious agents using the m-elisa. breeder flocks were sampled at lay, and broiler chicks were hatched from fertile eggs collected on the scheduled lay date of the breeders. the broiler chicks were placed for growout as ei ... | 1986 | 3015100 |
| application of the positive/negative ratio method of analysis to quantitate antibody responses to infectious bursal disease virus using a commercially available elisa. | an enzyme-linked immunosorbent assay (elisa) test kit has been developed for infectious bursal disease virus (ibdv), and it is widely used for the detection and quantification of ibdv antibody. with results obtained using the commercial kit, a method of analysis was performed utilizing a positive/negative (p/n) ratio standard curve. a prediction equation was derived from this analysis by which the elisa titer of a serum sample could be determined using one serum dilution. values obtained using t ... | 1986 | 3015105 |
| a comparison between the effect of an avian reovirus and infectious bursal disease virus on selected aspects of the immune system of the chicken. | reovirus 81-176 was inoculated subcutaneously into day-old specific-pathogen-free leghorns and evaluated for its effects on the immune system over a 3-week period. structural criteria included organ weights of the bursa of fabricius (bf) and spleen (sp), scoring of histological lesions in the bf, sp, and thymus, and hematological analyses of the circulating leukocytes. alterations in the functional capacity of the immune system were measured using the graft-versus-host reaction, the response of ... | 1986 | 3015115 |
| infectious bursal disease virus infection in the quail-chicken hybrid. | hybrids produced from crossing cornell k-strain white leghorn chickens and line ii japanese quails were studied for susceptibility to infection with infectious bursal disease virus (ibdv). quail-chicken hybrids were infected successfully following inoculation with ibdv at 14, 21, or 52 days of age. in most cases, precipitating antibodies were detected in serum by 10 days postinoculation (pi). although no clinical signs or gross lesions were evident in the bursa of fabricius of hybrids, histologi ... | 1986 | 3021100 |
| vaccination of day-old broiler chicks against newcastle disease and infectious bursal disease using commercial live and/or inactivated vaccines. | day-old broilers were administered live and/or inactivated vaccines to assess vaccine efficacy against challenge with newcastle disease (nd) and infectious bursal disease (ibd). chicks were from commercial breeder pullets vaccinated against nd and ibd using several live vaccine primers followed by an inactivated nd-ibd vaccine at 18 weeks. the most efficacious initial nd-ibd vaccination program was live nd virus by eye drop and live ibd vaccine injected subcutaneously (sq) followed 2 hours later ... | 1986 | 3021102 |
| subcutaneous clostridial infection in broilers. | a flock of 12,500 broilers 36 days of age experienced a sudden increase in mortality. post-mortem lesions were emphysema, severe enteritis, and a serosanguineous fluid in the subcutaneous tissue of the breast and thighs; there was no evidence of a loss in the integrity of the skin. clostridium perfringens and c. septicum were isolated from the affected subcutaneous tissue. histopathological and serological examination indicated previous infection with infectious bursal disease virus. the subsequ ... | 1986 | 3021106 |
| haematological values and changes in blood chemistry in chickens with infectious bursal disease. | haematological and blood serum chemical changes were studied in two groups of specific pathogen free chickens infected at five weeks old with different field isolates of infectious bursal disease virus. blood and serum components were determined five days after infection. there were significant decreases (p less than 0.05) in the total erythrocyte count, packed cell volume, haemoglobin concentration, albumin, albumin: globulin ratio, uric acid and glucose. serum components which increased signif ... | 1986 | 3010410 |
| an unusual case of inclusion body hepatitis in a cockerel. | this report describes inclusion body hepatitis in a 12-week-old leghorn-type cockerel. the cockerel had come from a specific-pathogen-free flock and was reared in a positive-pressure isolator until 6 weeks old. grossly, the cockerel was jaundiced, and the liver was swollen and had small white foci throughout. histologically, there were foci of hepatic necrosis and basophilic inclusion bodies in the nuclei of numerous hepatocytes. a fowl adenovirus, isolated from the liver, was found to belong to ... | 1986 | 3015107 |
| comparison of a kinetic-based enzyme-linked immunosorbent assay (kelisa) and virus-neutralization test for infectious bursal disease virus. ii. decay of maternal antibody in progeny from white leghorns receiving various vaccination regimens. | a computer-assisted single-serum-dilution indirect kinetic-based enzyme-linked immunosorbent assay (kelisa) was used for quantitating the natural decay rate of infectious bursal disease virus (ibdv) maternal antibody in progeny obtained from white leghorn breeders. the kelisa results were compared with those of a standard virus-neutralization (vn) test. pullets were subjected to two ibdv immunization regimens. group 1 was vaccinated at weeks 0, 2, and 10 with two live vaccines in drinking water ... | 1986 | 3015098 |
| predicted sequence of the host-protective immunogen of infectious bursal disease virus. | the genome of australian strain 002-73 of infectious bursal disease virus (ibdv) has been cloned as cdna fragments into an expression library based on pur plasmid vectors. recombinant colonies were selected with a monoclonal antibody specific for the 32-kda host-protective immunogen of ibdv and fully characterized by nucleotide sequence analysis. the amino acid sequence of several tryptic peptides derived from the native 32-kda structural protein has confirmed the coding region assignment and nu ... | 1986 | 3011501 |
| pathology of avian adenovirus serotypes in the presence of escherichia coli in infectious-bursal-disease-virus-infected specific-pathogen-free chickens. | ten strains of adenovirus representing 10 serotypes were administered intratracheally to 3-week-old specific-pathogen-free chickens, which also received 2.5 x 10(5) colony-forming units of a pathogenic escherichia coli intranasally. those birds had been inoculated by eye drop at 1 day of age with a virulent infectious bursal disease virus (ibdv). controls consisted of groups of chickens inoculated with: (a) ibdv and e. coli, (b) ibdv only, (c) e. coli only, and (d) no virus or e. coli. gross pul ... | 1986 | 3015109 |
| efficacy of coarse spray administration of commercial intermediate infectious bursal disease vaccines. | the efficacy of two intermediate infectious bursal disease (ibd) vaccines (clone vac d-78 and s-706) for immunizing specific pathogen-free (spf) white leghorn chickens by coarse spray (cs) against subclinical ibd was compared. both intermediate ibd vaccines were equally capable of immunizing day-old spf chickens by cs and were safe as evidenced by the absence of morbidity, mortality, or severe gross and microscopic bursal pathology at 28 days of age. | 1986 | 3016692 |
| infectious bursal disease immunisation failures in chickens in nigeria. | 1986 | 3016956 | |
| formation, characterization and interfering capacity of a small plaque mutant and of incomplete virus particles of infectious bursal disease virus. | serial undiluted passages of infectious bursal disease virus in chick embryo cells were accompanied by a von magnus type fluctuation of infectivity in viral harvests and a gradual decrease of plaque size. from the 9th undiluted passage on, the whole virus population consisted of small plaque-forming virus. the small plaque size remained constant when subsequent infections were carried out at low multiplicities. small plaque virus interfered with the replication of large plaque standard virus. th ... | 1986 | 3017017 |
| evaluation of the immunogenicity, stability, pathogenicity, and immunodepressive potential of four commercial live infectious bursal disease vaccines. | the study was divided into three experiments. in each, day-old specific pathogen free (spf) white leghorns were injected subcutaneously with one of the following commercial live infectious bursal disease (ibd) vaccines: burcell, bursine, clone vac d-78, and s-706. in the first experiment, the immunogenicity of each vaccine was determined by challenge with virulent ibdv at 25 days of age. all four vaccines were equally efficacious in preventing clinical ibdv infection. the second experiment deter ... | 1986 | 3018712 |
| quantitation of natural antibodies to infectious bursal disease in nigerian indigenous chickens. | serum samples collected from 687 indigenous chickens located in small scattered groups in four states of nigeria were examined for antibodies to infectious bursal disease (ibd) virus by the agar-gel precipitation (agpt) and counterimmunoelectrophoresis (cieop) tests. 51 of the positive samples were further titrated by each of the two techniques. cieop detected more positive reactors (74.59%) than agpt (58.95%). cieop also detected higher antibody levels among the reactors [geometric mean titre ( ... | 1986 | 3021387 |
| immunohistochemical detection of infectious bursal disease and infectious bronchitis viral antigens in fixed, paraffin-embedded chicken tissues. | immunoperoxidase and immunofluorescent techniques were used to detect and localise infectious bursal disease virus (ibdv) and infectious bronchitis virus (ibv) in fixed, paraffin-embedded chicken tissues. a modified bouin's solution (9 parts of 1% picric acid to 1 part of 10% neutral buffered formalin and 5% acetic acid) proved to be the fixative of choice. tissues fixed in 10% neutral buffered formalin showed heavy background staining, whereas enzyme treatment of these tissues succeeded in expo ... | 1986 | 18766540 |
| pathogenic and structural properties of wild type infectious bursal disease virus (ibdv) and virus grown in vitro. | large plaque (lp) and small plaque (sp) variants of infectious bursal disease virus (ibdv) which are formed in vitro after serial passages in ce-cells at low or high multiplicities of infection were tested for their pathogenic properties in susceptible chickens. lp virus caused clinical manifestations and destruction of the bursa of fabricius (bf) without killing the animals. no signs of a disease appeared after infection with sp virus, and only limited necrotic foci developed in the bf. coinfec ... | 1987 | 3028331 |
| challenge study on infectious bursal disease in chicks derived from vaccinated hens. | presence and levels of maternal antibody (ma) in broiler chicks derived from hens vaccinated with a live infectious bursal disease (ibd) vaccine were investigated by a quantitative agar-gel precipitin test. at day old 100% of the chicks tested had ma; by 17 days of age it was present in only 10%. the mean ma level at day old was 337.5 uk units/ml but decreased to 6.3 uk units/ml at 17 days of age. randomly selected chicks from the pool studied were challenged at weekly intervals from day old for ... | 1987 | 3037742 |
| experimental cryptosporidiosis in broiler chickens. | seven-day-old conventional broilers were inoculated either orally or intratracheally (it) with 2.5 x 10(5), 5.0 x 10(5), or 2.0 x 10(6) oocysts of cryptosporidium baileyi (32 birds for each dosage level per group; 192 birds total). thirty-two birds served as unninoculated controls. mean weekly weight gain and feed conversion were determined during a 5-week period. carcass pigment was graded using a roche color fan. fecal oocysts were calculated from random cage samples 6, 8, 11, 13, 15, 18, 20, ... | 1987 | 3601855 |
| replication of infectious bursal disease virus in continuous cell lines. | three mammalian continuous cell lines--ma-104, vero, and bgm-70--were tested for their ability to support replication of infectious bursal disease virus (ibdv). selected tissue-culture-adapted vaccine strains and tissue-culture-adapted field isolates of ibdv replicated in the ma-104, vero, and bgm-70 cells; cytopathic effects were most pronounced in the bgm-70 cells. the cytopathic effects of the viruses in bgm-70 cells and chicken embryo fibroblast (cef) cultures were similar. virus-neutralizat ... | 1987 | 3039967 |
| adenovirus infection associated with respiratory disease in commercial chickens. | the lesions and etiologic agents associated with 13 outbreaks of respiratory disease in commercial chickens were investigated. adenoviruses were isolated from tracheal and lung tissues of affected chickens in all 13 outbreaks. escherichia coli was isolated from the lung of an occasional bird. the tracheal specimens were consistently negative for bordetella avium, but e. coli and occasionally staphylococcus aureus were isolated. there was also serological evidence in one outbreak, and pathologica ... | 1987 | 2823779 |
| comparison of the effect of live newcastle disease vaccine clone 30 in broilers administered at day 1 or at day 7 and the effect of h120 vaccination at 17 days of age: a field experiment. | to analyse the results of a vaccination on the first day of age against newcastle disease (nd) and on the 17th day of age against infectious bronchitis (ib) resp. with spray vaccines with clone 30 and h120 vaccine. these vaccinations are compared in field circumstances with other vaccination methods. a serological examination and challenge test were used to be informed about the response and protection. from the present study the following conclusions can be drawn: clear indications are obtained ... | 1987 | 3031867 |
| molecular weight determination of the two segments of double-stranded rna of infectious bursal disease virus, a member of the birnavirus group. | the molecular weights (mol. wts.) of the two double-stranded (ds) rna segments of infectious bursal disease virus (ibdv) were determined using previously sequenced reovirus genes m3 and s2 as internal ds rna reference molecules. electrophoresis under fully denaturing conditions revealed mol. wts. of 2.26 x 10(6) daltons and 1.98 x 10(6) daltons. by direct length measurements under the electron microscope, using two different spreading conditions, the two segments were calculated to be composed o ... | 1987 | 3033456 |
| antigenic diversity of infectious bursal disease viruses. | statistically significant antigenic differences were detected among serotype i infectious bursal disease viruses (ibdv) using the virus-neutralization test. eight serotype i commercial vaccine strains, five serotype i field strains, and two serotype ii field strains were tested. hyperimmune guinea pig antisera against heterologous and homologous ibdv strains were used in cross-neutralization tests. relatedness values were calculated from geometric mean antibody titers based on a minimum of three ... | 1987 | 2831868 |
| comparison of two commercial enzyme-linked immunosorbent assays and conventional methods for avian serology. | sera tested for hemagglutination-inhibition (hi) activity against newcastle disease virus (ndv) and infectious bronchitis virus (ibv) and virus-neutralizing (vn) activity against infectious bursal disease virus (ibdv) and viral arthritis (va) virus were collected from a wide variety of accessions into the diagnostic services laboratory, poultry disease research center, university of georgia. the sera were then segregated according to hi or vn titer to ndv, ibv, ibdv, or va virus and stored froze ... | 1987 | 3034226 |
| effect of bursal cell number on the pathogenesis of infectious bursal disease in chickens. | the pathogenesis of infectious bursal disease (ibd) in chickens neonatally chemically bursectomized (cb) by cyclophosphamide and subsequently inoculated with various numbers of bursal cells was examined. cb chickens inoculated with at least 62.5 x 10(6) bursal cells were as susceptible to ibd clinical manifestations (as determined by gross and microscopic evaluation of bursal tissues, virus recovery from spleen, and antibody titer) as intact chickens following inoculation with virus at 5 weeks o ... | 1987 | 2823772 |
| allograft and antibody responses of 15i5-b congenic chickens. | seven congenic lines of chickens that differ from the parental inbred line rprl-15i5 for genes in the major histocompatibility (b) complex are under development. after 2 to 5 generations of crossing b-homozygous chickens were produced for interim histocompatibility and antibody competence tests. chickens in each of the b-congenic lines had antigens determining rapid skin graft rejection and stimulation of mixed lymphocyte responses by 15i5 chickens. several lines repeatedly developed different a ... | 1987 | 3037561 |
| mechanism of t cell immunosuppression by infectious bursal disease virus of chickens. | spleen cells of chickens infected with ibdv responded poorly to in vitro stimulation with con a. the mitogenic hyporesponsiveness was due to the presence of suppressor cells that could be removed by pretreatment of is cells with carbonyl iron or cytodex-3 microcarrier beads. addition of suppressor cells to normal spleen cells prevented the normal cells from responding to mitogen. cell-to-cell contact between responder and suppressor cells was not necessary for suppression to occur; the effect wa ... | 1987 | 3037563 |
| chicken b lymphocyte differentiation: ontogeny, bursal microenvironment and effect of ibd virus. | 1987 | 3037565 | |
| [aerogenic vaccination against infectious bursitis in chickens using "dessau" gumbovak]. | 1987 | 2831832 |