Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nonstructural proteins of herpes simplex virus. i. purification of the induced dna polymerase. | herpes simplex virus-induced dna polymerase purified by published methods was found to be contaminated with many others proteins, including virus structural proteins. thus, deae-cellulose and phosphocellulose chromatography were used in combination with affinity chromatography to purify dna polymerase from herpes simplex virus type 1- and type 2-infected cells. the purified enzyme retained unique features of the herpesvirus-induced dna polymerase, including a requirement for high salt concentrat ... | 1977 | 21304 |
| measurement of antibodies to herpesvirus types 1 and 2 in human sera by microradioimmunoassay. | the binding of 125i-labelled anti-human antibodies against the fc igg fragment to unlabelled antiviral immunoglobulins in the surface of infected cells was used to quantitate antibodies against herpes simplex virus type (hsv-1) and type 2 (hsv-2) in sera from patients with cervix carcinoma. the microradioimmunoassay technique (micro-ria) proved to be 5-10 times more sensitive than the microneutralization test. antibody titres determined by micro-ria correlated with neutralizing antibody titres t ... | 1977 | 22235 |
| enhancement of ige-mediated histamine release from human basophils by viruses: role of interferon. | human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen e or anti-ige. leukocyte cultures incubated with virus (i.e. hsv-1, influenza a, and adeno-1) but not exposed to ragweed antigen e or anti-ige fail to release histamine. if, however, leukocyte cultures are first exposed to virus and then to ragweed antigen e or anti-ige, significant enhancement of histamine release occurs. both infectious and inactivated virus enhance histamine release and the ... | 1977 | 67173 |
| detection of herpes simplex virus-related antigens in the nuclei and cytoplasm of biochemically transformed cells with peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence. | herpes simplex virus (hsv)-related antigens have been demonstrated in the nuclei and cytoplasm of human and mouse cells biochemically transformed by ultraviolet light-irradiated hsv. this was accomplished by using peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence with rabbit antisera that had high neutralizing titers against the hsv-specific thymidine kinase activity and virus infectivity. hsv-1 antisera reacted with antigens in cells biochemically transformed by ... | 1977 | 71279 |
| detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity. | skin fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ... | 1977 | 64461 |
| correlation of herpes simplex virus types 1 and 2 with clinical features of infection. | strains (338) of herpes simplex virus (hsv) were isolated in stockholm during 1965-1974. by immunoelectroosmophoresis it was possible to identify all strains as either hsv type 1 (hsv-1) or 2 (hsv-2). no strains of intermediate antigenic type or with untypable characteristics were found. the antigenic type of hsv was correlated with body site and clinical features of infection. a case of severe, recurrent, abdominal pain in association with hsv-2 infection is described. in one patient with acute ... | 1977 | 188954 |
| photodynamic treatment of herpes simplex virus during its replicative cycle. | photodynamic treatment of herpes simplex virus type 1-infected hamster embryo fibroblasts (lsh strain) with a low concentration of proflavine (0.08 mug/10(5) cells per ml), a 3-9-diamine acridine dye, inhibited production not only of infectious progeny but also of virion particles. however, there was no appreciable inhibition of viral or cellular dna synthesis, even when the infected cells were repeatedly exposed to this low concentration of dye and light during the replication cycle of the viru ... | 1977 | 189063 |
| anatomy of herpes simplex virus dna vii. alpha-rna is homologous to noncontiguous sites in both the l and s components of viral dna. | previous reports from this laboratory (honess and roizman, 1974) have operationally defined alpha polypeptides as the viral proteins that are synthesized first in hep-2 cells treated with cycloheximide from the time of infection with herpes simplex virus type 1 until the withdrawal of the drug 12 to 15 h after infection. it has also been shown that the viral rna (designated alpha rna) that accumulates in the cytoplasm during cycloheximide treatment and on polyribosomes immediately upon withdrawa ... | 1977 | 189068 |
| immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens. | lactoperoxidase-catalyzed radioiodination was used to study reactions between surface antigens and antibodies on bhk-21 cells infected with hsv-1 and hsv-2. isolation of iodinated surface antigens was achieved by indirect immune precipitation of triton x-100 disrupted cells with antisera to hsv and igg. analysis of immune precipitates by polyacrylamide gel electrophoresis (page) revealed at least 10 antigens, ranging in m.w. from 35 x 103 to 160 x 103 daltons. antigens were detectable on cell su ... | 1977 | 187696 |
| identification of virion polypeptides in hamster cells transformed by herpes simplex virus type 1. | ten polypeptides were detected on the surface of the virion of herpes simplex virus type 1. of these ten polypeptides, three were detected in hamster cells transformed by herpes simplex type 1. | 1977 | 189314 |
| cell-mediated immune responses in recurrent herpesvirus infections. i. lymphocyte proliferation assay. | studies in immunosuppressed and immunodeficient patients indicate that the cell-mediated immune response appears to be responsible for controlling reactivated herpesvirus infections. in this study, the various parameters of a herpesvirus (types 1 and 2) antigen specific lymphocyte proliferation assay were optimized and used to evaluate individuals with clinical, recurrent hsv-1 and hsv-2 infections. normal individuals with neutralizing antibody to hsv-1 or hsv-2 responded to virus antigen in cul ... | 1977 | 191528 |
| herpes simplex virus types 1 and 2 in clinical infections: differences observed by electron microscopy. | microtubule-like structures have been observed ultrastructurally in bhk-21/4 cells infected with herpes simplex virus type 2 (hsv-2) but not in cells infected with herpes simplex virus type 1 (hsv-1). bhk-21/4 cells were infected with two known strains of hsv-2 and one known strain of hsv-1, as well as with vesicular fluid from 50 clinical isolates (47 perioral, two penile, and one from the leg) in an examination of the accuracy of electron microscopy for differentiation between clinical isolate ... | 1977 | 191545 |
| inhibitory and lethal concentrations of 9-beta-d-arabinofuranosyladenine and its hypoxanthine-derivative versus herpes simplex virus, type 1. | minimum inhibitory concentrations of 9-beta-d-arabinofuranosyladenine (ara-a, adenine arabinoside, vidarabine) and a purified preparation of 9-beta-d-arabinofuranosylhypoxanthine (arabinoslhypoxanthine, ara-hx) at end points of 50% mic50) and 100% (mic100) reduction to challenges of approximately 50 p.f.u. of herpes simplex virus, type 1 (hsv-1) were determined in vero renal tissue cultures. adenosine deaminase is universally present in tissue cultures and serum. these same tests were repeated i ... | 1977 | 191546 |
| induction of deoxypyrimidine kinase activity in human embryonic lung cells infected with varicella-zoster virus. | deoxypyrimidine kinase (deoxythymidine [tdr] kinase and deoxycytidine kinase) activity was induced in human embryonic lung cells after infection with varicella-zoster virus (vz virus). increased enzyme activity was also produced by using cell-associated virus as inoculum instead of cell-free virus. anti-vz virus serum inhibited both the appearance of cytopathic effect and the induction of enzyme activity. the induced tdr kinase activity was more thermostable than that induced by herpes simplex v ... | 1977 | 191646 |
| complement-fixing antigen of human cytomegaloviruses. | the titer of complement-fixing (cf) antigen of human cytomegalovirus (cmv) strain c87 was not reduced by 10 cycles of freezing and thawing or by storage at 4 c for two months, although the virus was labile at 37 c and 100 c. the rapid increase in titers of cf antigen and plaque-forming units was seen from days 3 through 6 after infection; however, cf antigen was detected 24 hr after infection. the molecular weight of cf antigen was estimated by gel filtration to be greater than 1.5 x 10(7) dalto ... | 1977 | 190330 |
| effects of cytochalasin b on the maturation of herpes simplex virus type 1: an ultrastructural investigation. | 1977 | 190777 | |
| cellular immunity to herpes simplex virus in man. vii. k-cell activity to hsv1 infected target cells in disease. | herpes simplex virus-infected target cells, sensitised with antiviral antibody can be lysed by non-immune human lymphocytes (k-cells). this antibody dependent cell mediated immunity (adcc) is a very efficient technique for destroying foreign target cells and may play a role in maintaining the localization of recurrent herpetic infections. we have used 51cr labelled herpes virus infected target cells to examine the k-cell activity of peripheral blood lymphocytes in patients who have either malign ... | 1977 | 195708 |
| quantification of the herpes simplex virus dna present in biochemically transformed mouse cells and their revertants. | four cell lines biochemically transformed by u.v.-irradiated herpes simplex virus contain virus dna fragments ranging from 3 to 22% of the hsv genome. of five revertant clones selected for 3h-tdr or brdurd resistance, four had lost all detectable virus dna while the fifth, selected for brdurd resistance, retained the entire virus fragment but there was a reduction of virus copies per cell from 5 to 1. three 'supertransformed' revertant cell lines contained virus dna fragments ranging from 12 to ... | 1977 | 196037 |
| evidence that herpes simplex virus dna is transcribed by cellular rna polymerase b. | in herpes simplex virus type 1 (hsv-1)-infected hep-2 cells, amanitin added before or at various times after infection always reduced viral multiplication. also, the three waves of transcription of hsv-1 dna, which led to the synthesis of alpha, beta-, and gamma-polypeptides, were all sensitive to amanitin in hep-2 cells, and the amanitin-sensitive rna polymerase activities of isolated nuclei were equally sensitive to the inhibitor before and during the infection. on the contrary, hsv-1 dna tran ... | 1977 | 191658 |
| [investigations concerning the evidence of herpes simplex virus type 2 (hsv-2) antibodies in patients with carcinoma of the cervix (author's transl)]. | the neutralizing antibodies to hsv-1 and hsv-2 were determined in the sera of 128 patients. infection was detectable in nearly 100% of the cases in each of the three investigated groups (patients with carcinoma of the cervix, female patients with chronic recurrent hsv infection in the genital area and a control group without and history of hsv infection). the percentage of patients displaying hsv-2 antibodies in the group with carcinoma of the cervix (38%) is significantly higher than in the con ... | 1977 | 191994 |
| experimental herpes simplex virus type 1 encephalitis: treatment with 5-trifluoromethyl-2'-deoxyuridine. | 5-trifluoromethyl-2'-deoxyuridine (f(3)dthd) was evaluated for its neurotoxicity and for its ability to increase the life span of mice injected intracerebrally with herpes simplex virus type 1 (hsv-1) and f(3)dthd simultaneously. f(3)dthd showed no neurotoxicity at the highest concentration tested (100 mg/kg). mice injected intracerebrally with hsv-1 died within 5 days postinfection. however, all mice injected concurrently with hsv-1 and 100 mg of f(3)dthd per kg lived through the termination of ... | 1977 | 192143 |
| search for inhibitors against herpes simplex virus type-i in cell extracts derived from human lymphoblastoid cell lines. | cell extracts obtained from kb cells and 5 human lymphoblastoid cell lines including 2 from burkitt's lymphoma (p3hr-1 and raji), one each from nasopharyngeal carcinoma (no.223), acute lymphatic leukemia (molt-4) and a healthy person (nc-37) were tested for their inhibitory effects on the growth of herpes simplex virus type-1 (hsv-1) in green monkey kidney (gmk) cells by the plaque titration method. the relationship between the production of hsv-1 inhibitors and the degree of epstein-barr virus ... | 1977 | 196817 |
| protection from oral herpes simplex virus infection by a nucleic acid-free virus vaccine. | the effect of immunization with inactivated herpes virus vaccines, including a vaccine free of all nucleic acid, was investigated in a mouse model system. protection against oral lesions induced by herpes simplex virus type 1 was demonstrated by several criteria: (i) reduction in the incidence and severity of primary oral lesions; (ii) decrease in acute and latent infection of the regional sensory ganglia; and (iii) protection from viral encephalitis and death. the immune response of mice to the ... | 1977 | 197013 |
| effect of silica on the pathogenic distinction between herpes simplex virus type 1 and 2 hepatitis in mice. | the role of macrophages in the difference in liver pathogenicity between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in mice was investigated by selectively blocking the macrophage function of the mice by silica. intravenous administration of 3 mg of silica 2 h before virus inoculation partially abolished the difference between the two virus types, as judged by macroscopic and microscopic examination of the livers and by virus isolation studies. intraperitoneal inoculation of 50 mg of ... | 1977 | 197016 |
| genetics of resistance to phosphonoacetic acid in strain kos of herpes simplex virus type 1. | a dna- temperature-sensitive mutant of herpes simplex virus type 1 exhibiting thermolabile dna polymerase activity, tsd9, was shown to be resistant to phosphonoacetic acid (paa) when plated at the permissive temperature. ts+ revertants of tsd9 were paa sensitive and exhibited dna polymerase activity intermediate between that of the wild-type virus and tsd9, indicating that both temperature sensitivity and sensitivity to paa are controlled by the same gene. since the position of tsd9 on the exist ... | 1977 | 197275 |
| in vitro transcription of herpes simplex virus ang dna by e-coli rna polymerase. | hsv-1 ang dna and a defective genome of the same virus were transcribed with e. coli rna polymerase under various salt conditions. the extent of transcription was assayed by hybridizing the crna to the hind iii, hpa i and hind ii restriction fragments of the dna templates using the blot technique of e. southern. the transcripts proved to contain sequences homologous to all dna fragments. a similar ratio of hybridized crna and the amount of fragment dna was observed in all cases. the results sugg ... | 1977 | 197492 |
| anatomy of herpes simplex virus dna viii. properties of the replicating dna. | this paper concerns the properties of herpes simplex virus 1 dna replicating in hep-2 and human embryonic lung cells. the results were as follows. (i) only a small fraction of input viral dna entered the replicative pool. the bulk of the input viral dna cosedimented with marker viral dna and did not appear to be degraded or dissociated into l and s components. (ii) nascent dna sedimented faster and banded at a higher density than that of mature viral dna extracted from virions. pulse-chase exper ... | 1977 | 196115 |
| studies on the intracellular replicating dna of herpes simplex virus type 1. | 1977 | 196402 | |
| indirect immunofluorescence detection of human igm and igg antibodies against herpes simplex virus type 1 induced cell surface antigens. | an indirect immunofluorescence method, based on the use of infected hela cell coverslip cultures was developed to demonstrate human igm and igg class antibodies against herpes simplex virus (hsv) type 1 induced cell surface antigens. a total of 35 specimens from 20 patients have been tested; including patients with a clinical diagnosis of hsv type 1 or type 2 primary infection, patients with recurrent hsv infections, patients without any hsv infections, and patients with varicella-zoster virus ( ... | 1977 | 196479 |
| [isolation of high-molecular infectious dna of herpes simplex virus type 1]. | 1977 | 192379 | |
| [prevention and therapy of viral diseases with special reference to interferon]. | human leukocyte interferon (hli) was used for treatment of human diploid fibroblasts before and after infection with vaccinia virus, herpes simplex virus type 1 (hsv 1), herpes simplex virus type 2 (hsv 2), and varicella zoster virus (vzv). vero cells were infected with medical lake macaque herpes virus (mlmv), and treated with hli in the same way. in all of these systems hli exhibited an antiviral effect when administered before infection, and this effect could be increased by additional hli tr ... | 1977 | 192653 |
| role of macrophages in hepatitis induced by herpes simplex virus types 1 and 2 in mice. | a marked difference was found between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in the induction of hepatic necrotic lesions in mice inoculated intraperitoneally. although hsv-2 produced many large, progressive liver lesions in 4-week-old balb/c mice, hsv-1 only occasionally induced a few, self-limiting foci, which eventually healed. this was reflected in the isolation of hsv from the liver and spleen, two organs that are rich in macrophages. although hsv-1 could be only temporarily ... | 1977 | 192671 |
| solid-phase radioimmunoassay of human immunoglobulin m and immunoglobulin g antibodies against herpes simplex virus type 1 capsid, envelope, and excreted antigens. | a solid-phase radioimmunoassay developed in our laboratory for detection of human viral immunoglobulin m (igm) and igg antibodies was applied to demonstrate human class-specific antibody response against capsid, envelope, and excreted antigens of herpes simplex virus type 1. in primary infections, a clear igm and igg antibody response was found predominantly against the envelope components, whereas the igm and igg antibodies to the capsid antigen appeared more slowly. increasing igg antibody tit ... | 1977 | 192678 |
| morphological transformation of rat embryonic fibroblasts by abortive herpes simplex virus infection: increased transformation rate correlated to a defective viral genotype. | rat embryo fibroblasts were abortively infected with various stocks of herpes simplex virus type 1 strain ang at 42 degrees. in uninfected controls all of the cells died during an 8-day incubation period at the elevated temperature, whereas varying numbers of cells in the infected cultures survived and formed colonies during subsequent incubation for 3--4 weeks at 37 degrees. all of the survivors appeared to be morphologically transformed. two types of survivors, epithelial- and spindle-like cel ... | 1977 | 192697 |
| comparative complement fixation and serum neutralization antibody titers to herpes simplex virus type 1 and herpesvirus simiae in macaca mulatta and humans. | the serological relationship of herpes simplex type 1 virus and herpesvirus simiae was studied. antibody titers to these viruses were determined in 163 macaca mulatta sera and 67 human sera by serum neutralization (sn) and complement fixation (cf) tests. both groups of sera were also tested by cf with envelope and capsid antigens of herpes simplex type 1. by sn, the majority of the monkeys and all of the humans had a higher titer to herpes simplex type 1 than to h. simiae. by cf, with crude anti ... | 1977 | 192762 |
| a longitudinal study of varicella-zoster virus infections in renal transplant recipients. | a serum bank maintained for renal transplant recipients allowed for a longitudinal study of antibody responses before and after herpes zoster. renal transplant recipients without herpes zoster served as controls. antibody responses to varicella-zoster virus, herpes simplex virus type 1, and cytomegalovirus were measured. the serological responses following herpes zoster were prompt and sustained (in the majority of cases), transient, or not present at all. zoster without an eruption occurred (ap ... | 1977 | 192807 |
| effect of immunization on acute and latent infections of vaginouterine tissue with herpes simplex virus types 1 and 2. | inoculation of mice with herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) by the vaginal route resulted in viral shedding during the acute phase of the infection and the development of a latent infection in vaginouterine tissue lasting up to one year in greater than 25% of the animals. immunization with either serotype of hsv markedly reduced viral shedding when animals were subsequently challenged with the homologous serotype, but immunization with hsv-2 was significantly less effective in ... | 1977 | 192810 |
| comparative effects of herpes simplex virus types 1 and 2 in organotypic cultures of mouse dorsal root ganglion. | mature organized cultures of mouse dorsal root ganglion (mdrg) were infected with herpes simplex virus, type 1 (hsv 1) and type 2 (hsv 2). onset of infectious virus production occurred faster and reached higher levels in hsv 2-infected cultures. neurons, supporting cells and myelin were affected in both types of infection, but morphological changes occurred significantly earlier and more dramatically with the type 2 infection. the pattern of myelin changes was distinctly different in the two typ ... | 1977 | 192854 |
| polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel. | crossed immunoelectrophoresis was used to characterize herpes simplex virus type 1 (hsv-1) antigens produced by infected hep-2 cells. we report on a method for analyzing the polypeptide content in individual antigen-antibody precipitates eluted from the second-dimensional agarose gel. four glycoprotein antigens of hsv-1, ag-8, ag-11, ag-6, and ag-3, were isolated and analyzed for polypeptide content. the molecular weights of the polypeptides are presented. | 1977 | 192909 |
| inhibition of viral rna methylation in herpes simplex virus type 1-infected cells by 5' s-isobutyl-adenosine. | 5' s-isobutyl-adenosine (siba), a structural analogue of s-adenosylhomocysteine, reversibly blocks the multiplication of herpes simplex type 1 virus. in the presence of siba, viral protein synthesis is inhibited. after removing siba the synthesis of proteins starts rapidly again. the new polypeptides are mainly alpha proteins (honess and roizman, j. virol. 14:8-19, 1974,), normally the first to be synthesized after infection. the rapid synthesis of proteins after release of inhibition seems to b ... | 1977 | 192910 |
| induction of deoxycytidine deaminase activity in mammalian cell lines by infection with herpes simplex virus type 1. | herpes simplex virus type 1 induces deoxycytidine deaminase (cytidine/deoxycytidine aminohydrolase, ec 3.5.4.5) activity when it lytically infects a number of mammalian cell lines. the deaminase activity is induced in a mouse cell line that is deficient in this enzyme. the induction of the enzyme in this mutant cell line does not occur in the presence of actinomycin d and the induced enzyme is more thermolabile than the enzyme of the wild-type mouse cell line. furthermore, a new deoxycytidine de ... | 1977 | 193115 |
| herpesvirus group antibodies in children with hodgkin's disease. | during the period of three years ((1972-1974), serum samples from 60 patients (children and adolescents) with lympho-hematopoietic system diseases were examined for antibodies to all four human herpesviruses. among these were 26 active hodgkin's disease (ahd) patients and 6 hd patients with a minimum five years' remission. simultaneously matched controls (age, sex) of ahd patients were examined. antibody levels against the viral capsid antigen of epstein-barr virus (ebv/vca) in ahd patients were ... | 1977 | 193463 |
| biochemical and immunological characterization of deoxythymidine kinase of thymidine kinaseless hela cells biochemically transformed by herpes simplex virus type. | thymidine kinase (tk) from herpes simplex virus type 1 (hsv-1) biochemically transformed hela cells, purified by affinity chromatography, has been characterized with respect to its electrophoretic mobility, molecular weight, activation energy, substrate specificity, and immunological specificity. tk purified from hsv-1-transformed hela cells has the same electrophoretic mobility as tk purified from hela cells lytically infected with hsv-1. the sedimentation velocity of purified tk from transform ... | 1977 | 193790 |
| thymidine kinaseless revertants of ltk- cells transformed by herpes simplex virus type 1 are resistant to retransformation by homologous virus. | mouse l cells lacking thymidine kinase (ltk-) that had been transformed to the thymidine kinase-positive (tk+) phenotype by herpes simplex virus type 1 (hsv-1) were cultured in medium containing tritiated thymidine. six clonal lines of cells surviving this treatment were found to have the following properties: (i) the cells were tk- and had spontaneous back-reversion frequencies to the tk+ phenotype of 10(-5) or less, (ii) the cells contained hsv antigens, although in lesser amounts than in the ... | 1977 | 193795 |
| efficacy of herpes simplex virus type 1 immunization in protecting against acute and latent infection by herpes simplex virus type 2 in mice. | icr mice were immunized with herpes simplex virus type 1 (hsv-1) and later challenged with hsv-2 by footpad inoculation. both immunized animals and age-matched, nonimmunized controls were observed for ascending neurological disease and latent infection of spinal ganglia resulting from the hsv-2 challenge. control animals had a 78% incidence of acute and latent infection compared with a 1.7% incidence in immunized mice. the data show immunity to hsv-1 is protective against both acute and latent i ... | 1977 | 193798 |
| herpes simplex virus resistance and sensitivity to phosphonoacetic acid. | phosphonoacetic acid (paa) inhibited the synthesis of herpes simplex virus dna in infected cells and the activity of the virus-specific dna polymerase in vitro. in the presence of concentrations of paa sufficient to prevent virus growth and virus dna synthesis, normal amounts of early virus proteins (alpha- and beta-groups) were made, but late virus proteins (gamma-group) were reduced to less than 15% of amounts made in untreated infected cells. this residual paa-insensitive synthesis of gamma-p ... | 1977 | 189089 |
| incidence and distribution of herpes simplex virus types 1 and 2 from genital lesions in college women. | during a 9-month period, 9,772 women were treated at the student health center's gynecology clinic. herpes simplex virus was isolated from 30 of 57 patients clinically diagnosed as suffering from a herpetic or herpetic-like genial infection for a virological incidence rate of 0.31%. using virus plaque diameter in chick embryo cells and heat stability of viral thymidine kinase, 37% of the isolates were classified as herpes simplex virus type 1 and 63% were classified as herpes simplex virus type ... | 1977 | 204741 |
| clinical evaluation of commercial conjugates for direct immunofluorescence of herpes simplex virus. | commercially prepared antibody conjugates that are now available allow extensive application of direct immunofluorescence for diagnosing herpetic infections. five of these conjugates--microbiological associates bivalent herpes antiserum (mba-bivalent), mba anti-herpes types 1 (mba-1) and 2 (mba-2), and flow laboratories anti-herpes types 1 (flow-1) and 2 (flow-2)--were compared for antibody titer, diagnostic accuracy, and nonspecific background staining on known infected tissue. four of these we ... | 1977 | 73546 |
| anatomy of herpes simplex virus dna. ix. apparent exclusion of some parental dna arrangements in the generation of intertypic (hsv-1 x hsv-2) recombinants. | we are reporting the physical location of parental dna sequences in 28 recombinants produced by crossing herpes simplex viruses (hsv) 1 and 2. the parental crosses were of two kinds. in the first, temperature-sensitive mutants of hsv-1 and hsv-2 were crossed to produce wild-type recombinants. in the second, temperature-sensitive mutants of hsv-1 rendered resistant to phosphonoacetic acid were crossed with wild-type hsv-2, and recombinants that multiplied at nonpermissive temperature and were res ... | 1977 | 198577 |
| studies on the in vivo methylation of replicating herpes simplex virus type 1 dna. | 1977 | 199990 | |
| inhibition of antigen and mitogen-induced lymphocyte transformation by fetal calf serum. | use of fetal calf serum as a serum supplement in whole blood microcultures of human lymphocytes resulted in a significant suppression of in vitro stimulation with herpes simplex virus (type 1) antigen, purified protein derivative, and phytohemagglutinin. if the response to viral antigen is weak in autologous serum it may be completely missed if cultures are carried out in fetal calf serum. | 1977 | 200328 |
| scanning electron microscopy of the surfaces of hamster embryo cells transformed by herpes simplex virus. | the surfaces of normal hamster embryo fibroblast (hef) cells were examined by scanning electron microscopy. surface characteristics of hef cells were compared to those of cells derived from a primary tumor induced in hamsters following s.c. inoculation of herpes simplex virus type 1-transformed hef cells (14-012-8-1) and to the surfaces of cells derived from a metastatic tumor to the lung induced by the same cells. the most obvious difference in the surface characteristics of the examined cells ... | 1977 | 200348 |
| interaction between cyclic nucleotides and herpes simplex viruses: productive infection. | infection of human fibroblasts and hep-2 cells with herpes simplex virus type 1 (hsv-1) produced a decrease in the intracellular levels of cyclic adenosine 5'- monophosphate (camp) and a concomitant increase in the cyclic guanosine 5'- monophosphate (cgmp) levels. in both cell cultures, changes in cyclic nucleotide levels were first observed at 6 h after viral inoculation and were maximal at 12 h. in human fibroblasts, the addition of theophylline, dibutyryl camp, or papaverine (camp-enhancing c ... | 1977 | 200559 |
| [herpes simplex virus (hsv-1 and hsv-2) infection: its clinical and oncogenic properties (author's transl)]. | the epidemiology and clinical features of diseases caused by the herpes simplex virus, are reviewed and recent results are discussed which give an insight into the complex mechanism of primary and chronic, recurrent hsv-1 infections. immunological reactions in hsv infection and data concerning the oncogenic potential of hsv-1 and -2 are dealt with. furthermore, current therapeutic possibilities are outlined. | 1977 | 201107 |
| antiviral activity of arabinosylthymine in herpesviral replication: mechanism of action in vivo and in vitro. | 1-beta-d-arabinofuranosylthymine (ara-t), a metabolite of the sponge tethya crypta, has shown selective activity against herpes simplex virus (hsv) replication (g. a. gentry and j. f. aswell, virology 65:294-296, 1975). analysis of hsv-infected and uninfected cell lysates by cscl isopycnic centrifugation showed that ara-t blocked the incorporation of [(3)h]hypoxanthine into viral deoxyribonucleic acid and, to a large extent, into host deoxyribonucleic acid of infected (but not uninfected) cells. ... | 1977 | 197886 |
| temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genome. | 1977 | 198141 | |
| cell-mediated cytotoxicity to herpes-infected cells in humans: dependence on antibodies. | herpes simplex virus type 1 (hsv-1)-infected human skin fibroblasts were used as target cells in a 51cr release assay, using peripheral blood mononuclear cells from hsv-seropositive and -seronegative donors as effector cells. cytotoxicity was exerted by ordinarily prepared lymphoid cells but could be reduced by extensive washing of the effector cells. the antibody dependence of the system was shown by the recovery of activity through addition of positive serum or medium used for the early washes ... | 1977 | 194838 |
| effector cell involved in cell-mediated cytotoxicity to cells infected with herpes simplex virus type 1. | experiments were performed to analyze the characteristics of the effector cells involved in cell-mediated killing of herpes-infected fibroblasts. effector cells were fractionated according to adherence and phagocytic properties, and lymphocytes were depleted of t-cells, and ea- and eac- (antibody-coated bovine erythrocytes and bovine erythrocytes coated with rabbit anti-bovine antibodies and c5-deficient mouse serum, respectively) rosette-forming cells by differential centrifugation. column pass ... | 1977 | 194839 |
| treatment of fatal disseminated herpes simplex virus, type 1, infection in immunosuppressed mice (39899). | 1977 | 198818 | |
| [herpetic infection of the oral cavity in guinea pigs]. | a convenient experimental model for serial studies of herpes simplex of the buccal mucosa has been developed in guinea pigs by application of fresh isolates of herpes simplex virus type 1 from a patient with primary herpetic gingivo-stomatitis on prescarified buccal mucosa. the herpetic nature of the eruptions in the oral cavity was confirmed by virus isolations and serologically. the possibility of exogenous reinfection of the buccal mucosa in convalescent animals against the background of humo ... | 1977 | 198963 |
| specifically immune mouse t-cells can destroy h-2 compatible murine target cells infected with herpes simplex virus types 1 or 2. | lymphocytes from the spleen (sl) or peritoneal cavities (pl) of hsv-1- or hsv-2-immunized c 3 h mice expressed pronounced cytotoxicity, as measured by 51cr release assay, against l cells experimentally infected with hsf-types 1 or 2 but not against uninfected l cells. hsv-1 or hsv-2 immune lymphocytes induced substantially more 51cr release when l-cell targets were infected with homotypic virus compared to those infected with heterotypic virus. inasmuch as the cytotoxicity of specifically immune ... | 1977 | 198986 |
| comparison of igg level and fluorescent antibody titre to herpes simplex virus type 1 in cerebrospinal fluid and serum of encephalitis patients and controls. | 1977 | 199683 | |
| quantitation of herpes simplex virus type 1 rna in infected hela cells. | we have quantitatively analyzed the size and amount of herpes simplex virus (hsv)-specific rna synthesized in hela cells using dna and rna excess hybridization. at 2 h after infection (early), transcripts from 20% of the total hsv dna are present on polyribosomes as poly(a+) rna. at this time, viral poly(a+) rna comprises 60 to 75% of the newly synthesized poly(a+) mrna on polyribosomes. by 6 h after infection (late), poly(a+) hsv rna transcribed from 35 to 40% of the viral dna is found on polyr ... | 1977 | 191652 |
| immunoprecipitation of herpes simplex virus type 1 antigens with different antisera and human cerebrospinal fluids. | rabbit convalescent and hyperimmune sera, human patient and blood donor sera, as well as cerebrospinal fluids of humans with herpes simplex virus encephalitis all recognize similar major antigenic components in herpes simplex virus infected rabbit or human cells as shown by electrophoretic analysis of immunoprecipitates. besides the main glycoproteins with an apparent molecular weight of 100,000 (peak i) the antisera precipitate glycoproteins in a region of an apparent mol. wt. of 60,000--80,000 ... | 1977 | 192180 |
| in vitro and in vivo inhibition of virus multiplicaton by microwave hyperthemia. | the effect of microwave hyperthermia (41 degrees and 43 degrees c) on virus multiplication have been explored in vitro (hsv-1 infected primary rabbit kidney cultures) and in vivo (mice infected with hsv-1 or vaccinia). in vitro the cells were inoculated with hsv-1 and heated to 41 degrees or 43 degrees c either before or after infection. virus yields were significantly decreased when the cells were exposed to hyperthemia within the first few hours after infection, while hyperthemia was without e ... | 1977 | 192183 |
| cell cycle dependency of murine cytomegalovirus replication in synchronized 3t3 cells. | synchronized murine 3t3 cells have been used to investigate the possible dependency of murine cytomegalovirus replication upon the cell cycle. the normal latent period of 12 h characteristic of asynchronous 3t3 cells was protracted to more than 24 h after an early g1 infection in synchronous cells. in this case viral progeny were not detected until after the initiation of the host s-phase. cells maintained in the g1 phase did not replicate virus. this failure could not be explained by a decrease ... | 1977 | 194054 |
| regulation of herpesvirus macromolecular synthesis. vi. synthesis and modification of viral polypeptides in enucleated cells. | cells were enucleated with cytochalasin b after infection with herpes simplex virus 1. when protein synthesis was blocked by cycloheximide from the time of infection, mrna for viral alpha-infected cell polypeptides (icp) 4, 0, and 27 accumulated in the cytoplasm and was expressed after the removal of both drug and nucleus. a host protein, icp 22, whose synthesis is stimulated in intact cells, was not made, and viral protein icp4, which is normally modified to a form that migrates more slowly in ... | 1977 | 195087 |
| cell-free synthesis of herpes simplex virus proteins. | polyribosomes isolated from herpes simplex virus type i (hsv-1)-infected cells have been used to program a eucaryotic cell-free translation system. at least 10 hsv-specific polypeptides, with apparent molecular weights of 25,000 to 160,000, are synthesized by wild-type hsv-infected polyribosomes. polyribosomes prepared from thymidine kinase-negative mutants of hsv direct the synthesis of three putative nonsense termination polypeptides. hsv-specific polypeptides synthesized in vitro are precipit ... | 1977 | 195089 |
| inhibition of herpesvirus dna synthesis by 9-beta-d-arabinofuranosyladenine in cellular and cell-free systems. | 9-beta-d-arabinofuranosyladenine 5'-triphosphate (ara-atp) is an inhibitor both of dna polymerase-alpha and -beta from noninfected rabbit kidney cells and of the dna-dependent dna polymerase induced by herpes simplex virus type 1 (strain ies). the studies were performed with partially purified enzymes, and each of the different polymerase preparations contained only one dna-dependent dna polymerase species. these enzymes were inhibited in a competitive manner. the hsv-induced dna-dependent dna p ... | 1977 | 212980 |
| arabinosyl-n6-hydroxyadenine: a new potent antivirus drug. | abrabinosyl-n6-hydroxyadenine (ara-ha) was tested for its antiherpesvirus and immunosuppressive activities in vivo and in vitro. mouse strains were used that had been shown earlier to be very susceptible to intraperitoneal (i.p.) or intracerebral (i.c.) inoculation of a virulent strain of herpes simplex virus type 1 (hsv-1). susceptible mice were inoculated i.p. or i.c. with a stock pool of hsv-1 and treated with ara-ha (i.p.) beginning at least 24 hr later. the mice were given a 10-day course o ... | 1977 | 212981 |
| a rational approach to the development of antiviral chemotherapy: alternative substrates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) thymidine kinase (tk). | 1977 | 212988 | |
| ammonium chloride inhibits cell fusion induced by syn mutants of herpes simplex virus type 1. | cell fusion induced by syn mutants of herpes simplex virus type 1 is inhibited by nh4cl. the inhibition of fusion is both rapid and rapidly reversible and apprears to be due to the nh4+ ion. virus production was not significantly altered by nh4cl, although cell growth was greatly diminished. | 1977 | 196105 |
| 5'-terminal and internal methylated nucleosides in herpes simplex virus type 1 mrna. | rna labeled with [methyl-3h]methionine and/or [32p]orthophosphate was isolated from the polyribosomes of herpes simplex virus (hsv) types 1-infected cells and separated into polyadenylylated [poly(a+)]and non-polyadenylylated [poly(a-)] fractions. virus-specific rna was obtained by hybridization in liquid to either excess hsv dna or filters containing immobilized hsv dna. analysis in denaturing sucrose gradients indicated that hsv-specific poly(a+) rna sedimented in a broad peak, with a modal s ... | 1977 | 196108 |
| activation of latent herpesvirus hominis in explants of rabbit trigeminal ganglia: the influence of immune serum. | more than fifty albino rabbits were inoculated into the right scarified cornea with 10(7) pfu of the kupka strain of human herpes virus type 1 (hhv-1). at intervals ranging from 4--280 days post infection (p.i.), both gasserian ganglia, both trigeminal nerve trunks and pieces from brain stem and from both corneas were explanted. activation of the latent hhv-1 was found mainly in the homolateral ganglion tissue, but also in explants originating from the opposite ganglia. within 24--72 hours, prio ... | 1977 | 192182 |
| lymphocyte stimulation by herpes simplex virus (hsv) antigens. 1. culture conditions, cell fractionation and presentation of hsv antigens. | optimal culture conditions for rabbit lymphocytes were established. inclusion of 2-mercaptoethanol in the medium greatly enhanced responses. lymphocytes from the blood of hsv-immunized rabbits responded specifically in vitro to heat-killed (56 degree, 60 min) or uv-inactivated hsv preparations. a uv dose of 13,392 ergs/mm2 was the most suitable inactivating dose. oral intramucosal injection was the most effective way of generating blood lymphocyte responses to hsv, but animals immunized in trade ... | 1977 | 216529 |
| [in vitro studies of the antiviral activity of ammonium humate against herpes simplex virus type 1 and type 2 (author's transl)]. | ammonium humate, isolated from peat water, is a higher molecular polyphenolic compound with a strong antiviral activity against herpes simplex virus type 1 and type 2. three cell lines were chosen for examining the cytotoxicity of ammonium humate: rabbit kidney primary cells, hep-2- and fl-cells. as the light microscopic examination of both, the monolayers and the stained cover-glass cultures, revealed, concentrations of up to 5 microgram/ml ammonium humate can be used without hesitation for rab ... | 1977 | 203143 |
| type specificity and use of human sera for rapid typing of herpes simplex virus isolates by an indirect peroxidase-labeled antibody technique: a comparison with three other methods. | human sera from patients showing seroconversion following a primary herpes simplex virus type 1 (hsv-1) or hsv type 2 (hsv-2) infection, as well as 19s and 7s fractions obtained from the same sera, have been used for typing 191 hsv isolates by an indirect peroxidase-labeled antibody (pla) method. typing has also been performed on all of the isolates using a microneutralization (mn) and the indirect hemagglutination (iha) inhibition test. furthermore, 30 isolates have been typed by a plaque metho ... | 1977 | 196572 |
| antibodies against herpes simplex virus type 1 subunit antigens in patients with trigeminal neuralgia and controls. | serum igg antibody levels against herpes simplex virus (hsv) type 1 subunit (capsid, envelope, and excreted) antigens detected with radioimmunoassay were compared in 25 patients with trigeminal neuralgia and their age- and sex-matched controls. no significant differences were found between the patients and controls, either in the distribution of the antibody titers or in the mean titers against any of the subunit antigens tested. in 6 patients hsv antibody titers were tested before and after tri ... | 1977 | 197767 |
| [125i]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase. | [125i]deoxycytidine was a good substrate for herpes simplex virus type 1 thymidine kinase (tk), whereas [125i]deoxycytidine was a very poor substrate for cellular tk. simple, sensitive, and specific assays for viral tk could be carried out in vivo and in vitro even in the presence of cell tk. autoradiographic detection of incorporated [125i]deoxycytidine provided a rapid and simple method for detection of and screening for viral tk in infected as well as viral tk-transformed cells. | 1977 | 198581 |
| solid-phase enzyme immunoassay for herpes simplex virus. | an enzyme immunoassay was used for the rapid detection of herpes simplex virus type 1 (hsv-1). the test utilized specific antibodies to hsv-1 attached to a solid phase. a laboratory preparation of hsv-1 was detectable in amounts of greater than or equal to 50 50% tissue culture-infective doses by this method. tests performed with clinical samples indicated a specificity of 95%. the sensitivity appeared to depend on the length of time the samples were stored. when samples were stored frozen for t ... | 1977 | 198493 |
| herpes simplex virus dna polymerase as the site of phosphonoacetate sensitivity: temperature-sensitive mutants. | temperature-sensitive (ts) mutants in a number of complementation groups of herpes simplex virus type 1 (hsv-1) are deficient in dna polymerase induction at the restrictive temperature. twenty-two mutants in 15 complementation groups were tested for sensitivity to phosphonoacetate (paa), a compound that inhibits hsv replication in vivo and the dna polymerase in vitro. one mutant, tsd9, was resistant to paa (pr), whereas all others were sensitive. revertants of tsd9 to the ts+ phenotype simultane ... | 1977 | 199734 |
| alterations of neutral glycolipids in cells infected with syncytium-producing mutants of herpes simplex virus type 1. | the isolation of syncytium-producing mutants of herpes simplex virus type 1 (kos strain), which cause extensive cell fusion during otherwise normal infections, has been reported previously (s. person, r. w. knowles, g. s. read, s. c. warner, and v. c. bond, j. virol. 17:183-190, 1976). seven of these mutants, plus two syncytial strains obtained elsewhere, were used to compare the incorporation of labeled galactose into neutral glycolipids of mock-infected, wild-type-infected, and syncytially inf ... | 1977 | 199742 |
| comparison of the development of resistant strains of type 1 herpes simplex virus to in vitro antiviral activity of 5-iodo-2'-deoxyuridine or ribavirin. | exposure of hsv/1 to low concentrations of ribavirin during 4--5 passages does not produce ribavirin-resistant virus. idu resistance was developed by hsv/1 while it was being passed simultaneously. this resistance was seen to develop in both kb and vero cells. the idu-resistant virus is also resistant to ribavirin in kb cells. | 1977 | 212974 |
| herpes simplex infection and experimental allergic encephalomyelitis. an experimental model system for reactivation of eae. | the effects of herpes simplex virus type 1 (hsv-1) infection on the course of experimental allergic encephalomyelitis (eae) were studied in rats. fifty percent of animals given two intracerebral injections of hsv-1, one before and one after induction of eae, showed clinical and pathologic evidence of recently exacerbated eae 16 days after the second hsv-1 injection. when hsv-1 injections were administered subcutaneously before and after induction of eae, 45% of survivors showed pathologic change ... | 1977 | 559269 |
| comparative evaluation of human leukocyte and fibroblast interferon in the prevention of herpes simplex virus keratitis in a monkey model. | the prophylactic potency of topically applied human leukocyte interferon and human fibroblast interferon was compared in a monkey model with herpes simplex virus type 1-induced keratitis. both interferons effectively prevented keratitis at 1.9 x 10(6) u of activity per ml but were ineffective at 1.9 x 10(3) u/ml. slight differences between human leukocyte interferon and human fibroblast interferon observed at the level of 1.9 x 10(5) u/ml were not statistically significant. | 1977 | 408274 |
| fluorescent antibody study of diseased, end-stage human kidneys. | forty cases of diseased kidneys at end-stage were studied by fluorescent antibody technique in search for viral etiology of glomerulonephritis and other renal diseases. among these 40 cases, 12 (30%) were ascribed to immune complex disease because of detection of immunoglobulins and complement in glomeruli of the same kidney specimen. in 8 cases (20%) only complement was detected in glomeruli. in the remaining 50% neither complement nor immunoglobulin deposit was found in glomeruli. the etiologi ... | 1977 | 357100 |
| psychopharmaca and electroconvulsive therapy in relation to viral antibodies and interferon. experimental and clinical study. | chlorpromazine (chp) in a concentration of 8.3 microgram per ml medium inhibited attachment and multiplication of cec and of various cell lines. the same chp concentration applied to grown cec monolayers inhibited the growth of tbev but not the growth of hhv 1, while interferon was slightly stimulated. the clinical course of fatal subcutaneous tbev infection in mice was not affected by daily chp treatment while brain and serum interferon were stimulated. antibody formation in tbev infected or hh ... | 1977 | 202214 |
| selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine. | a guanine derivative with an acyclic side chain, 2-hydroxyethoxymethyl, at position 9 has potent antiviral activity [dose for 50% inhibition (ed(50)) = 0.1 mum] against herpes simplex virus type 1. this acyclic nucleoside analog, termed acycloguanosine, is converted to a monophosphate by a virus-specified pyrimidine deoxynucleoside (thymidine) kinase and is subsequently converted to acycloguanosine di- and triphosphates. in the uninfected host cell (vero) these phosphorylations of acycloguanosin ... | 1977 | 202961 |
| further studies on the association of herpes simplex virus type 1 dna with host dna during productive infection. | our previous finding of an association between herpes simplex virus type 1 (hsv-1) and african green monkey kidney (bsc-1) dnas in productively infected cells has been extended by investigating the influence of the time course, temperature, and multiplicity of infection on the extent of this association. the interaction of host and viral dnas was not only dependent on the concentration of intracellular viral dna, but also upon the particular conditions of infection. an examination of the time co ... | 1977 | 18625492 |
| physical mapping of herpes simplex virus-coded functions and polypeptides by marker rescue and analysis of hsv-1/hsv-2 intertypic recombinants. | a number of temperature-sensitive (ts) mutants and one pyrimidine deoxyribonucleoside kinase-deficient mutant of herpes simplex virus (hsv), have been located on the physical map of the genome by means of marker rescue experiments and by the analysis of the crossover points in intertypic recombinants between hsv types 1 and 2. the physical map is compared to the genetic map and certain anomalies identified. analysis of infected-cell polypeptides specified by intertypic recombinants has allowed t ... | 1978 | 221322 |
| inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants. | herpes simplex virus type 1 dna synthesis and infections progeny production were studied in five different conditional hamster (bhk-21) cell cycle mutants. at the nonpermissive temperature (39.5 degrees c), both events were strongly inhibited in four of these cell lines. the degree of inhibition was a reproducible characteristic of each cell mutant and in two cases was dependent upon the multiplicity of infection. experiments involving shifts to the nonpermissive temperature at least 3 h postinf ... | 1978 | 202752 |
| herpes simplex virus types 1 and 2 in ocular disease. | in a continuous series of 457 patients with presumed herpetic eye disease, virus isolation and typing revealed 154 patients with herpes simplex virus type 1 and three patients with type 2 infections. in 219 isolates that were examined for neutralization by specific antisera, for growth in human fibroblasts, and, in part, for temperature sensitivity, there were found substantial strain differences in addition to the type-specific characteristics. the clinical features of each of the three type 2 ... | 1978 | 203255 |
| the thymidine kinase gene of herpes simplex virus type 1: cell-free protein synthesis and substrate specificity studies. | the hsv thymidine kinase genetic system has a number of advantages for the study of virus and cell genetics. both forward and reverse mutants are readily selected. the enzyme can be assayed directly in the presence of the cell tk by the choice of the appropriate substrate, for example, 125idc. the gene product, hsv tk, can be identified by sds-polyacrylamide gel electrophoresis and mutants can be found with alterations in the electrophoretogram. the in vitro synthesis of hsv tk in response to hs ... | 1978 | 221338 |
| effect of cyclic nucleotides on the response of cells to infection by various herpesviruses. | the effect of varying the intracellular cyclic nucleotide concentration of mouse cells infected with hsv-1 or of duck embryo fibroblasts infected with marek's disease virus has been studied. it has been shown in each case that agents that elevate the intracellular camp levels increase the yield of infectious virus or viral dna, but inhibit cell division. cgmp enhances synthesis of viral dna but allows continued synthesis of cell dna. | 1978 | 221343 |
| the use of intertypic recombinants for analysis of gene organization in herpes simplex virus. | analysis of the dna sequence arrangement and polypeptides specified by 28 hsv-1 x hsv-2 recombinants show the following: (i) recombinants with heterogeneous l and s components or with heterogenous inverted repeats are viable. (ii) hsv-1 and hsv-2 genes appear to be functionally equivalent and with few exceptions co-linearly arranged. co-linear dna maps have been established. (iii) at most two arrangements of hsv dna are capable of replication. this is consistant with current studies suggesting t ... | 1978 | 221347 |
| mapping of the herpes simplex virus dna sequences in three herpes simplex virus thymidine kinase-transformed cell lines. | we have made use of a novel filter hybridization approach in order to map the herpes simplex virus (hsv) dna sequences which are present in three hsv thymidine kinase (tk)-transformed cell lines. the cell line 33a+ which was produced by infection of 3t3 tk- cells with uv-irradiated hsv-2 (333) was found to contain one contiguous stretch of viral dna sequences which maps between 0.15 and 0.57 on the hsv-2 genome. the sequences mapping from 0.31 to 0.37 were present in 3--4-fold higher abundance t ... | 1978 | 221351 |
| enhanced efficiency of transfection of hsv dna in hsv-1 dna-transformed hamster cells. | the efficiency of transfection, i.e., the number of plaques per microgram of dna, was compared for herpes simplex virus (hsv) type 1 and type 2, and herpesvirus eidolon (antigenically unrelated to hsv) dnas in hsv dna-transformed cells (eh/a44) and control hamster cells (eht). the efficiency of transfection of hsv-1 and hsv-2 dnas was significantly higher in eh/a44 cells than in eht cells, showing that an early step in hsv infection was involved in the partial resistance of hsv dna-transformed c ... | 1978 | 221357 |
| the dna of serially passaged herpes simplex virus: organization, origin, and homology to viral rna. | high-density dna prepared from serially passaged herpes simplex virus contains three major classes of modified viral dna molecules. the altered dna molecules are composed of multiple repetitions of sequences derived from the right-hand side of the s region of the parental plaque-purified viral dna. the repeat units contained in the three types of high-density dna share most of their dna sequences but differ with respect to a small region derived from the unique sequences of the s component of hs ... | 1978 | 221364 |
| inhibition of the multiplication of herpes simplex virus by aliphatic nitrosamines. | non-toxic doses of dimethylnitrosamine and diethylnitrosamine inhibit the multiplication of herpes simplex virus type 1 and type 2 in hep-2, wi-38 and primary hek cells. these results are somewhat unexpected, since both hep-2 and wi-38 cells lack detectable microsomal enzyme activity. the possible significance of the preliminary results is discussed. | 1978 | 221368 |
| herpes simplex virus vaccine: protection from stomatitis, ganglionitis, encephalitis and latency. | a mouse model system was developed for studying the pathogenesis of oral infection with herpes simplex virus type 1 and the protection offered by prior immunization with a nucleic acid-free vaccine. of non-immunized mice, 95-100% developed ulcerative lesions 3-5 days following application of virus to abraded oral epithelial surfaces. infection of the ipsilateral sensory (trigeminal) ganglion and the cerebellum occurred by day 2 and sequentially progressed to the contralateral ganglion by day 4 a ... | 1978 | 221370 |