Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| parasites elicited cross-reacting antibodies to opisthorchis viverrini. | two batches of crude antigens extracted from adult opisthorchis viverrini worms were compared. one was derived from adult worms harvested from the livers of laboratory infected hamsters and another was obtained from worms sedimented from the faeces of opisthorchiasis patients following treatment with praziquantel. sds-page and coomassie brilliant blue staining revealed that the two preparations had similar protein components of which the predominant ones were the 17-18 kda doublet. the antigens ... | 1997 | 9346276 |
| evaluation of bithynia funiculata snail antigens by elisa-serodiagnosis of human opisthorchiasis. | four batches of crude somatic antigens from: (1) opisthorchis viverrini adult worms, (2) bithynia funiculata-whole body, (3) b. funiculata-head-foot, and (4) b. funiculata-visceral mass were assayed against sera from 81 opisthorchiasis patients, 30 parasite-free healthy individuals, and 50 individuals infected with other helminthic infections, and their antibody levels determined. by igg-elisa, the antigenic reactive proteins were found in both the head-foot and the visceral mass of b. funiculat ... | 1997 | 9561614 |
| application of hazard analysis critical control point (haccp) as a possible control measure for opisthorchis viverrini infection in cultured carp (puntius gonionotus). | opisthorchiasis due to opisthorchis viverrini and transmitted through infected freshwater cyprinoid fish (carps) affects more than 8 million people in thailand, people's democratic republic of lao, and vietnam. the hazard analysis critical control point (haccp)-concept has been recommended by fao and who to be included in programs to control foodborne trematode infections (fbt). haccp is a multifactorial approach to control food hazards through surveillance of diseases, foods, and operations and ... | 1997 | 9656352 |
| metacercariae isolated from cyprinoid fishes in khon kaen district by digestion technic. | several kinds of fresh-water fishes collected from natural water resources in khon kaen district were digested with 0.25% pepsin a to obtain the infective stage or metacercariae of fish-borne trematodes. most of them harbored at least 3 species of trematodes. two families of medical trematodes, i.e., family heterophyidae and family opisthorchiidae, were isolated. the heterophyid flukes consisted of haplorchis taichui, h. pumilio and centrocestus formosanus. opisthorchis viverrini was the only pa ... | 1997 | 9656383 |
| seasonal variation of opisthorchis viverrini infection in cyprinoid fish in north-east thailand: implications for parasite control and food safety. | reported is the seasonal pattern of opisthorchis viverrini metacercariae in cyprinoid fish in north-east thailand. samples of fish were collected in 1991-92 at monthly intervals from two areas-khon kaen province, where the opisthorchiasis transmission rate was high, and mahasarakham province, where the rate was low. metacercarial loads in both study areas had similar seasonal patterns. high burdens occurred in the late rainy season and winter (july to january) with low burdens during the summer ... | 1997 | 9185364 |
| opisthorchis viverrini infection in thailand: studies on the morbidity of the infection and resolution following praziquantel treatment. | a community study on opisthorchiasis was conducted in prachinburi province in eastern thailand during 1990-1992. the morbidity from opisthorchiasis in the community and reversibility of biliary pathology following treatment with praziquantel at a single dose of 40 mg/kg were assessed by longitudinal investigations of clinical, laboratory, and ultrasonographic changes. a total of 913 voluntary subjects infected with opisthorchis viverrini were randomly selected for longitudinal study, and 579 sub ... | 1997 | 9129534 |
| enhanced liver cell mutations in trematode-infected big blue transgenic mice. | parasite infections in humans have long been associated with specific types of cancers. schistosoma hematobium is a known inducer of urinary bladder cancer, helicobacter pylori is a gastric carcinogen, and hepatitis b virus and opisthorchis viverrini are causative agents of liver cell cancers. another liver fluke, fasciola hepatica, has also been identified as a neoplastic risk agent, primarily in animals. we used f. hepatica as a model agent to determine if the presence of an aggressive liver f ... | 1998 | 9685695 |
| opportunistic protozoa in stool samples from hiv-infected patients. | a retrospective study of stool samples of hiv-infected patients from january 1994 to december 1995 submitted to the department of tropical pathology was analyzed. there were twenty-two cases, all of which presented with chronic diarrhea. result showed that 50% were infected with protozoa. these include microsporidium (27.27%), cryptosporidium (9.09%), isospora belli (4.54%) and giardia intestinalis cysts (9.09%). other infections were candida sp, strongyloides stercoralis larva and opisthorchis ... | 1998 | 9740264 |
| opisthorchiasis and intestinal fluke infections in northern thailand. | four hundred and thirty-one residents from 16 provinces in northern thailand who had previously been found positive for opisthorchis viverrini or opisthorchis viverrini-like eggs were given praziquantel 40 mg/kg. the stool was collected for 4 to 6 times and examined for adult worms. the prevalence of opisthorchis viverrini in this group was 11.6%. intestinal flukes, haplorchis taichui and haplorchis yokogawai, were predominantly found in 63.11% and 10.44% respectively. other intestinal flukes (c ... | 1998 | 9740284 |
| brief survey of common intestinal parasites in the tokyo metropolitan area. | since 1955, when sanitary conditions were poor, the incidence of intestinal parasitism has steadily decreased. similarly, the number of requests for fecal examinations by physicians has also decreased. however, in our hospital, the incidence of parasites detected in fecal material has been increasing since 1994, regardless of the decreasing number of stool exams performed. possible reasons for this situation can be summarized as follows: first, an effective drug for treating trichuris trichiura ... | 1998 | 9796183 |
| specific gravity of opisthorchis viverrini eggs. | the specific gravity of the eggs of the liver fluke opisthorchisviverrini was determined using a sucrose gradient centrifugation and found to range from 1.2713 to 1.3043. the peak egg count was located at the sucrose fraction with a specific gravity of 1.2814. an attempt to float eggs in saturated sodium nitrate solution, sp.gr. 1.4, failed. examination of human stool specimens for oviverrini eggs by simple flotation in saturated sodium nitrate solution and the formol-ether sedimentation techniq ... | 1998 | 9858635 |
| treatment of opisthorchis viverrini and intestinal fluke infections with praziquantel. | the study was carried out from september to november 1997 in phrae province of northern thailand. a total of 95 adult patients with opisthorchis-like ova in their stools were randomly treated with two different manufactured praziquantels. group 1, consisting of 49 patients, received a single dose of 40 mg per kg praziquantel manufactured by the thai government pharmaceutical organization. group 2 (46 patients) received biltricide at the same dosage. haplorchis taichui, h. yokogawai, echinostome ... | 1998 | 9886106 |
| opisthorchis viverrini metacercaria in thai freshwater fish. | examination for metacercaria in freshwater fish, the common intermediate hosts of opisthorchis viverrini was carried out during 1992-1996. the 4-year survey of fish from markets in 14 provinces revealed that metacercariae of o. viverrini were found in fish from udon thani, sa kaeo and prachin buri provinces; fish from aranyaprathet district had the highest positive rates (25-28%). fish from 12 provinces were found to be positive with heterophyid metacercariae, namely: haplorchis pumilio, h. taic ... | 1998 | 9886121 |
| epidemiology of opisthorchiasis and national control program in thailand. | opisthorchiasis is a disease caused by opisthorchis trematode commonly known as liver fluke. in thailand opisthorchis viverrini is the only parasite of opisthorchiasis, the first case of opisthorchiasis was reported in 1911 by leiper from the autopsy of corpse in chiang mai. later on sadun in 1953, harinasuta and vajjarasthira in 1961, and wykoff in 1965 had demonstrated a complete life cycle of o. viverrini. history of opisthorchiasis control has dated back to 1950 as a small scale helminthiosi ... | 1998 | 9886122 |
| modulation of dihydroxy-di-n-propylnitrosamine-induced liver lesion development in opisthorchis-infected syrian hamsters by praziquantel treatment in association with butylated hydroxyanisole or dehydroepiandrosterone administration. | the effects of praziquantel coupled with dehydroepiandrosterone (dhea) or butylated hydroxyanisole (bha) administration 16 weeks subsequent to dihydroxy-di-n-propylnitrosamine (dhpn) treatment and infection with opisthorchis viverrini (ov) on lesion development in the liver of syrian hamsters were investigated. animals were given 80 ov metacercariae and then two i.p. injections of dhpn (500 mg/kg body weight) 4 and 5 weeks thereafter. at week 16, groups received praziquantel (250 mg/kg, i.g.) an ... | 1998 | 9914779 |
| bacterial and parasitic cholangitis. | bacterial cholangitis is a clinically defined syndrome caused by the regurgitation of infected bile into the circulation. the pathogenic mechanism is unclear, and systemic sepsis may not occur. prerequisite conditions are the presence of microorganisms in the bile and increased biliary pressure. bacteria that commonly cause cholangitis are escherichia coli, klebsiella, enterococcus, enterobacter, pseudomonas, and anaerobes. although most infections are polymicrobial, this situation may not alway ... | 1998 | 9581592 |
| use of delipidized antigens of taenia solium metacestodes in igg-elisa for detection of neurocysticercosis. | the development of igg-elisa for detecting neurocysticercosis is aimed at the routine laboratory, and requires a particular antigen preparation, an acceptable number of serum samples to be tested (both homologous and heterologous) and patients with a diversity of helminthic infections to rule out cross-reactions. this study characterizes igg-antibodies from cases of neurocysticercosis by assaying the sera against ether-delipidized antigens (5 microg/ml) prepared from metacestodes of taenia soliu ... | 1998 | 10437960 |
| relationships between the synthesis of n-nitrosodimethylamine and immune responses to chronic infection with the carcinogenic parasite, opisthorchis viverrini, in men. | this study investigated the relationship between immune responses to infection with the liver fluke, opisthorchis viverrini, and the synthesis of the carcinogen, n-nitrosodimethylamine (ndma) in humans. it also examined associations between synthesis of nitric oxide (no) and nitrosation of amines, in vivo. antibody and t cell responses to fluke antigens and post-alcohol urinary ndma excretion were assessed among three groups of 40-50 men with no, moderate and heavy liver fluke infection. markers ... | 1998 | 9525284 |
| parasitic infections among southeast asian labourers in taiwan: a long-term study. | parasitic infections have been reported to be relatively common among the southeast asian labourers in taiwan. this study, conducted in 1992-6, was designed to determine the temporal changes of the prevalence. faecal specimens were examined by the formalin-ethyl acetate sedimentation technique and blood samples screened using the quantitative buffy coat technique and confirmed by giemsa stained blood smear. the overall prevalence of intestinal parasitic infections was 10.3%. the annual prevalenc ... | 1998 | 9528821 |
| a small-scale survey of intestinal helminthic infections among the residents near pakse, laos. | a small-scale epidemiological survey was undertaken on the residents along the mekong river near pakse, laos, to know the status of helminthic infections. a total of 137 fecal samples were collected from the staffs of the provincial government, their family, and primary schoolchildren in pakse city, champassak province, and examined by kato-katz smear technique. the overall helminth positive rate was 75.9%, and the helminths detected were opisthorchis viverrini (43.8%). ascaris lumbricoides (26. ... | 1998 | 9529864 |
| an epidemiological survey on intestinal parasite infection in khammouane province, lao pdr, with special reference to strongyloides infection. | to estimate the current prevalence of intestinal helminth infection in lao pdr, a parasitological survey was conducted in three villages in khammouane province in december 1996, with special reference to strongyloides infection. a total of 669 fecal samples were collected in the villages and examined by agar-plate culture method and kato-katz thick smear method. intestinal helminths were demonstrated in 82% of the samples as follows: ascaris lumbricoides (30.5%), trichuris trichiura (23.7%), hoo ... | 1998 | 10772552 |
| chromosomes and c-banding of opisthorchis viverrini. | chromosome of opisthorchis viverrini was observed by air-drying and c-banding techniques. the chromosome number was 2n=12 and n=6 consisting of one large-sized metacentric, one medium-sized metacentric, one small-sized metacentric, one small-sized submetacentric or subtelecentric, one small-sized subtelocentric or acrocentric and one small-sized acrocentric. the relative lengths of the chromosomes were 32.02 +/- 2.52, 23.28 +/- 1.98, 15.24 +/- 3.40, 13.39 +/- 3.11, 10.18 +/- 1.56 and 5.82 +/- 0. ... | 1999 | 10774673 |
| prevalence of trematode metacercariae in cyprinoid fish of ban pao district, chiang mai province, northern thailand. | the prevalence of trematode metacercariae in natural cyprinoid fish was carried out in ban pao district, chiang mai province, northern thailand. fish were collected from both man-made water reservoirs and natural sources during july 1996-may 1997. nine species of cyprinoid fish harbored the metacercariae of opisthorchis viverrini and small intestinal flukes of the family heterophyidae, ie haplorchis taichui, h. pumilio and centrocestus spp. metacercariae of h. taichui were found approximately 38 ... | 1999 | 10774710 |
| potassium permanganate staining for differentiation the surface morphology of opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs. | potassium permanganate staining method was developed for differentiation opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs. the surfaces of o. viverrini, h. taichui and p. bonnei eggs stained permanently and temporarily were similar in appearance even the staining procedures were varied both in concentration and time. determined under light microscope set at 400x, all of these eggs were oval-shaped, operculated at one pole and indistinct small knob at posterior end. o. viv ... | 1999 | 10774711 |
| current status of infection-related gastrointestinal and hepatobiliary diseases in thailand. | the objective of this overview is to assess the present situation with regards to gastrointestinal and hepatobiliary diseases prevailing in thailand. in that context, special emphasis has been put on those forms of viral hepatitis prevalent in the region, namely, hepatitis a the frequency of which has undergone a change from hyper- to hypoendemic with a resulting decline in naturally acquired immunity; hepatitis b with its tendency to cause chronic liver disease mainly due to asymptomatic infect ... | 1999 | 10695796 |
| volatile nitrosamines and tobacco-specific nitrosamines in the smoke of thai cigarettes: a risk factor for lung cancer and a suspected risk factor for liver cancer in thailand. | in thailand, smoking of commercial cigarettes and of handmade cigarettes has drastically increased in recent decades. cancer of the lung and of the upper aero-digestive tract have also increased in thailand as they have in many other countries. it is our working hypothesis that the increase of primary cancer of the liver, especially of cholangiocarcinoma in the north-eastern provinces of thailand is associated with the use of tobacco in men infested with the liver fluke opisthorchis viverrini (o ... | 1999 | 9934860 |
| exposure to n-nitroso compounds in a population of high liver cancer regions in thailand: volatile nitrosamine (vna) levels in thai food. | the recent case-control studies in thailand indicate that a high incidence of liver cancer in thailand has not been associated with common risk factors such as hepatitis b infection, aflatoxin intake and alcohol consumption. while the infestation by the liver fluke opisthorchis viverrini (ov) accounted for the high risk in north-east thailand, there was no such exposure in the other regions of the country where the incidence of liver cancer is also high. case-control studies suggest that exposur ... | 1999 | 10418946 |
| mechanisms of biliary carcinogenesis: a pathogenetic multi-stage cascade towards cholangiocarcinoma. | carcinomas of the biliary tract are rare cancers developing from the epithelial or blast-like cells lining the bile ducts. a variety of known predisposing factors and recent experimental models of biliary carcinogenesis (e.g., infection with the liver fluke opisthorchis viverrini, models of chemically induced carcinogenesis and experimental models of pancreaticobiliary maljunction) have elucidated different stages of this complex system of biliary tumorigenesis. chronic inflammatory processes, g ... | 1999 | 10436802 |
| relationship between parasite-specific antibody responses and intensity of opisthorchis viverrini infection in hamsters. | the kinetics of parasite-specific antibody responses in relation to worm burden and egg output were investigated in hamsters infected with 25, 50 and 100 opisthorchis viverrini metacercariae (mc). levels of antibody to egg, excretory-secretory (es) and somatic antigens were examined by elisa on days 1, 3, 7, 14 and month 1 postinfection (p.i.), and repeated monthly up to 6 months. the antibody responses were first detected as early as 14 days after infection. hamsters that were infected with 100 ... | 2000 | 10672195 |
| investigation on subclinical aspects related to intestinal parasitic infections among thai laborers in taipei. | the migration of foreign workers from developing regions to developed countries may potentially lead to transmission of intestinal parasitic infections. in order to determine the relationship between intestinal parasitic infections and the health status of foreign workers, 302 thai laborers brought to taiwan were examined in this study. nine species of parasites were found in 64.9% of laborers; opisthorchis viverrini, hookworm, strongyloides stercoralis, giardia lamblia, trichuris trichiura, fas ... | 2000 | 11305242 |
| ultrastructural and immunohistochemical analysis of cholangiocarcinoma in immunized syrian golden hamsters infected with opisthorchis viverrini and administered with dimethylnitrosamine. | utilizing the experimental model in syrian golden hamsters, we explored the role of immunization in carcinogenesis. the animals, which were infected with liver flukes (opisthorchis viverrini), and administered a subcarcinogenic dose of dimethylnitrosamine, developed cancer. pre-immunizing with a crude somatic antigen did not reduce cancer development, but accelerated carcinogenesis. histopathological analysis of the cancer tissues was done once at week 30 and again at week 39 using h and e stain ... | 2000 | 11426579 |
| promotion of the health of rural women towards safe motherhood--an intervention project in northeast thailand. | an intervention project focusing on the health of women in the reproductive age was conducted in three districts of khon kaen province, northeast thailand between 1991 and 1996. main emphasis was placed on improving reproductive health, the nutritional status including the iron deficiency anemia (ida) as well as iodine deficiency disorders (idd), and the parasitic diseases liver fluke (opisthorchis viverrini) and hookworm. for implementation a community based primary health care approach was use ... | 2000 | 11488446 |
| opisthorchis viverrini: ultrastructure and cytochemistry of the glycocalyx of the tegument. | the ultrastructure and cytochemistry of the glycocalyx of the tegument of opisthorchis viverrini during maturation from newly excysted juvenile to adult stages were investigated using colloidal iron, ruthenium red and lectin stainings. the results showed that the glycocalyx was intensely stained by the first two dyes, thus indicating the presence of relatively high amounts of negative charges. however, the thickness and intensity of the staining decreased during the fluke's maturation. binding s ... | 2000 | 10831050 |
| localisation of parasite antigens and inflammatory responses in experimental opisthorchiasis. | the time course localisation of parasite antigens and related host pathology were studied in hamsters infected with 100 metacercariae of opisthorchis viverrini for up to 6 months. parasite antigens, as detected by immunofluorescence and/or immunoperoxidase-staining, were first observed in the flukes and the biliary epithelium of the intrahepatic and extrahepatic bile ducts as early as day 3 p.i. antigens increased as the parasite matured, both in tissues in direct contact with the flukes and tho ... | 2000 | 10856508 |
| partial characterization of a 17 kda protein of clonorchis sinensis. | a 17 kda protein from clonorchis sinensis adults was purified by a procedure including sephacryl s-200 hr gel filtration and q-sepharose anion exchange chromatography. the protein was proved to be a cysteine protease as it showed hydrolytic activity toward cbz-phe-arg-amc in the presence of dithiothreitol and was inhibited by specific inhibitors such as iodoacetic acid or trans epoxy-succinly-l-leucyl-amido(4-guanidino) butane. the polyclonal antibody raised against the protein reacted to 17 kda ... | 2000 | 10905071 |
| an epidemiological study on opisthorchis viverrini infection in lao villages. | the prevalence of liver fluke, opisthorchis viverrini, infection in rural and urban communities was studied in khammouane province in lao pdr. the infection was the commonest among the villagers examined, showing the positive rates of 52.9% and 55.0% in two rural communities and 60.7% in an urban community, respectively. the infection rate reached up to 20% or more within 4 years after birth and increased with age to a plateau in the age group over 20 years. the highest prevalence rate was over ... | 2000 | 11023079 |
| development of a pcr-based method for the detection of opisthorchis viverrini in experimentally infected hamsters. | opisthorchis viverrini infection is an endemic disease that causes a serious public health problem in southeast asia, especially in northeast thailand. we have developed a pcr method using a pair of primers named ov-6f/ov-6r for detecting o. viverrini eggs in stool samples and compared it with stoll's egg-count method. the primers were designed based on the pov-a6 specific dna probe sequence which gave a 330 base pair product. the pcr method can detect a single egg in artificially inoculated fae ... | 2001 | 11272648 |
| trematodes of the family opisthorchiidae: a minireview. | examination of the original descriptions of genera placed within the family opisthorchiidae has revealed that only 33 of the original 43 genera are valid members of this family. further study of these descriptions should also reveal that many of the subfamilies are also invalid. with reference to the original descriptions of these genera, and subsequent literature, a brief survey of the family has been compiled. information on the spectrum of definitive hosts that these helminths parasitize is p ... | 2001 | 11590910 |
| analysis of laci mutations in big blue transgenic mice subjected to parasite-induced inflammation. | parasite infections have long been associated with specific types of human cancers. schistosoma hematobium is an inducer of urinary bladder cancer, helicobacter pylori is a gastric carcinogen, and hepatitis b virus and opisthorchis viverrini are causative agents of hepatocellular carcinoma. another liver fluke, fasciola hepatica, has also been identified as a neoplastic risk agent, primarily in animals. we used f. hepatica-induced inflammation in mice to determine if the presence of an aggressiv ... | 2001 | 11733073 |
| parasitic infections and hepatic neoplasia. | parasitic infections are highly prevalent in the general population. detecting a relationship between a parasitic infection and cancer is not an easy task; it requires epidemiological, microbiological and molecular biology techniques. in this article the important mechanisms by which parasitic infections may induce carcinogenesis are reviewed. the association between schistosoma japonicum, schistosoma mansoni and hepatocellular carcinoma is covered. the cause-and-effect relationship between the ... | 2001 | 11935088 |
| intestinal parasitic infections in thai hiv-infected patients with different immunity status. | one of the major health problems among hiv seropositive patients is superimposed infection due to the defect of immunity. furthermore, intestinal parasite infection, which is also one of the basic health problems in tropical region, is common in these patients. in this study, a cross sectional study to document the prevalence of intestinal parasitic infection in thai hiv-infected patients with different immune status was performed. | 2001 | 11394966 |
| affinity purified oval antigen for diagnosis of opisthorchiasis viverrini. | monoclonal antibodies (mab) were raised against an oval antigen of the liver fluke opisthorchis viverrini which is the causative agent of a parasitosis, i.e. opisthorchiasis in thailand. the antibodies were used in an affinity column to purify the o. viverrini oval antigen from a crude extract of adult parasites by chromatography. the oval antigen was then used in a membrane (dot) elisa for detecting antibodies in serum samples of parasitologically confirmed opisthorchis viverrini infected indiv ... | 2001 | 12009074 |
| diagnosis of intestinal amebiasis using salivary iga antibody detection. | attempts were made to use soluble antigen extract of strain hk-9 of entamoeba histolytica to detect salivary iga antibodies in intestinal amebiasis patients by using elisa. total salivary samples of 109 individuals were divided into four groups. group i comprised 32 patients whose stools were positive only for e. histolytica cysts and/or trophozoites. group ii comprised 12 individuals whose stools were positive for e. histolytica and other intestinal parasites. group iii comprised 36 individuals ... | 2001 | 12041582 |
| nucleotide sequence of mitochondrial co i and ribosomal its ii genes of opisthorchis viverrini in northeast thailand. | the mitochondrial cytochrome c oxidase subunit i (co i) gene and the second internal transcribed spacer region (its ii) gene of opisthorchis viverrini were compared among o. viverrini from various areas in northeast thailand. the nucleotide sequences of partial co i gene (417 bp) of o. viverrini differed among o. viverrini originated from ubon ratana, leongpleuy, ban phai, maha sarakham, and chatturat. these intraspecific variations were classified into 5 patterns but no area-specific pattem was ... | 2001 | 12041584 |
| prevalence of opisthorchis viverrini infection among villagers harboring opisthorchis-like eggs. | the precise occurrence of opisthochis viverrini infection in humans, who were positive for opisthorchis-like eggs in a stool examination, was determined using the potassium permanganate staining method. in the 68 specimens examined, there were more individual o. viverrini eggs (38.24%) than singular haplorchis taichui eggs (29.41%). one-fourth of the total specimens contained a mixed infection of o. viverrini and h. taichi eggs. the median ratio of o. viverrini: h. taichui eggs in mixed infectio ... | 2001 | 12041596 |
| the present situation of paragonimiasis in endemic area in phitsanulok province. | a cross-sectional survey and evaluation of paragonimiasis situation from endemic area in phitsanulok province was studied. studies on the species and prevalence of parasites which infected people in noen maprang, phitsanulok province were also conducted during october 1999-march 2000. the sputum specimens were collected and examined to identify paragonimus heterotremus eggs. in addition fecal samples were collected and examined for parasites by the formalin-ethyl acetate concentration technique. ... | 2001 | 12041605 |
| epidemiology of liver cancer in thailand. | the cancer registry is an essential part of any rational programme of cancer control. the information is the primary resource for epidemiological research and for planning and evaluating health services for the prevention, diagnosis and treatment of cancer. epidemiological research, based on comprehensive cancer registration, remains the most valid and efficient way to plan and evaluate all aspects of cancer control. the estimated incidence of primary liver cancer in thailand is very high, liver ... | 2001 | 12718642 |
| gall bladder and extrahepatic bile duct changes in opisthorchis viverrini-infected hamsters. | opisthorchis viverrini infection is associated with several hepatobiliary diseases, but few reports have described extrahepatic lesions in opisthorchiasis. we therefore sequentially investigated histological changes of the gall bladder and extrahepatic bile duct in hamsters infected with 25 (group 1), 50 (group 2) and 100 (group 3) metacercariae for up to 180 days. acute inflammatory reactions, including congestion, neutrophil and eosinophil infiltration, occurred in the gall bladder as early as ... | 2002 | 12062790 |
| liver fluke-associated cholangiocarcinoma. | infection with liver flukes has been reported to be associated with bile duct malignancy. | 2002 | 12153620 |
| prevalence of parasitic infections among thai patients at the king chulalongkorn memorial hospital, bangkok, thailand. | parasitic diseases are still considered to be a major public health problem. most patients with parasitic infections are asymptomatic and therefore remain undetected. asymptomatic parasitic infections are usually discovered by routine parasite examination. to determine the result of parasite examination at the parasitology unit, out patient department, king chulalongkorn memorial hospital, bangkok, thailand, the authors collected the data of individuals examined for parasite infections from june ... | 2002 | 12188445 |
| a cross-sectional study of intestinal parasitic infections among schoolchildren in nan province, northern thailand. | a cross-sectional study of the prevalence of intestinal parasitic infections at eight schools in bo klau district and four schools in chalerm prakiet district, nan province, in january and february, 2001. a total of 1,010 fecal samples were examined using the formalin-ether sedimentation technique. results revealed that the rate of helminthic infection was 60.0%, while protozoa accounted for 36.2% of infections; mixed infections were common, resulting in a total prevalence of both parasites of 6 ... | 2002 | 12236415 |
| serodiagnosis of human opisthorchiasis using cocktail and electroeluted bithynia snail antigens. | cocktail and electroeluted antigens from bithynia goniomphalos, the snail intermediate host of opisthorchis viverrini, were extracted and purified. the performance of these two antigens in the antibody detection of human opisthorchiasis was evaluated by indirect elisa. serum samples from people whose stool was either: (i). positive for opisthorchis eggs (n=61); or (ii). positive for at least one of 19 other species of parasite (n=125); or (iii). clear of parasites (n=30) were tested. the sensiti ... | 2002 | 12243778 |
| detection of opisthorchis viverrini in human stool specimens by pcr. | 2002 | 12354909 | |
| expression of tenascin in bile duct cancer of hamster liver by combined treatment of dimethylnitrosamine with opisthorchis viverrini infections. | tenascin is an extracellular matrix glycoprotein known to be an essential factor for the modulation of reciprocal interactions between the epithelium and mesenchyme during embryogenesis and tumourigenesis. the interactions between the expression of tenascin in the liver of syrian golden hamster and the development of bile duct cancer in an opisthorchis viverrini-associated cholangiocarcinoma model were investigated. the tenascin was expressed in connective tissues surrounding the dilated ducts, ... | 2002 | 12363380 |
| rifampin markedly decreases plasma concentrations of praziquantel in healthy volunteers. | praziquantel is extensively metabolized by the hepatic cytochrome p450 (cyp) enzymes. the cyp3a isoforms are likely to be major enzymes responsible for praziquantel metabolism. rifampin (inn, rifampicin), a potent enzyme inducer of cyp-mediated metabolism (especially cyp2c9, cyp2c19, and cyp3a4), is known to markedly decrease plasma concentrations and effects of a number coadministered drugs. the aim of this investigation was to study the possible pharmacokinetic interaction between rifampin and ... | 2002 | 12426514 |
| high prevalence of fasciolopsis buski in an endemic area of liver fluke infection in thailand. | there is a high prevalence of fasciolopsis buski in sawasdee village in the nam som district, udonthani province in northeastern thailand, an endemic area of liver fluke infection. from stool examination of 183 villagers participating in the study, the authors detected a fluke parasitic infection rate of 14.8% (27 cases). of interest, the authors found a similar high prevalence of both opisthorchis viverrini (16 cases, 8.7%) and f buski (13 cases, 7.1%). this region is the endemic area of liver ... | 2002 | 12466749 |
| a survey of metacercariae in cyprinoid fish in nakhon ratchasima, northeast thailand. | metacercariae were recovered from freshwater fish taken from a stream in lum cha muak, nakhon ratchasima province, northeast thailand during the year 2000. seventy-nine cyprinoid fish comprising 3 species (52 puntius leiacanthus, 22 cyclocheilichthys armatus, and 5 hampla dispar) were collected. the prevalence of infection with metacercariae was 51.9%. only two genera of metacercariae were found: haplochinae spp and heterophyidae spp. haplochinae metacercariae were found in all 3 species of fish ... | 2002 | 12971487 |
| a survey of trematode cercariae in bithynia goniomphalos in northeast thailand. | a survey of trematode larvae in the fresh water snail, bithynia goniomphalos in the main water reservoir of huai thalaeng district, nakhon ratchasima province, thailand, was conducted. the study site is in an area with a high prevalence of opisthorchis viverrini infection. malacological samplings were made during the year 2000 to study the prevalence and intensity of infection. parasitological examinations of 527 live snails were performed at the veterinary parasitology laboratory, chulalongkorn ... | 2002 | 12971488 |
| parasitic infections in thai workers that pursue overseas employment: the need for a screening program. | stool examination is a requirement of the screening program for thai workers seeking overseas employment. we report on the prevalence of intestinal parasitic infections among these workers; the stool examination results of 2,213 thai workers who visited the out-patients department of the king chulalongkorn memorial hospital between september 2000 and january 2001 were analysed. one hundred and thirty-five (6.1%) were found to be infected with 1 or 2 species of parasite. there were 54 (40%), 47 ( ... | 2002 | 12971489 |
| motility of minute intestinal fluke, haplorchinae spp, metacercariae in fish dishes prepared by different uncooked methods. | fish-borne trematode is a worldwide problem, with the number of people infected by liver flukes alone estimated at 21 million. in addition to the major liver flukes, opisthorchis viverrini and clonorchis sinensis, several types of intestinal flukes also use cyprinoid fish as the intermediate host. traditional ingestion of undercooked and uncooked fish preparations is a major factor in pathogen acquisition. | 2002 | 11965210 |
| specific and common antigens of clonorchis sinensis and opisthorchis viverrini(opisthorchidae, trematoda). | the antigenic characterizations and serological reactions of human liver flukes, clonorchis sinensis and opisthorchis viverrini, were analyzed by immunoblot. the antigenic profiles of the crude extract of clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kda, and those of opisthorchis 34-37, 43, 70, and 100 kda. of these, the 8, 26-28 and 34-37 kda bands of clonorchis and the 100 kda of opisthorchis were major components of each excretory-secretory antigen. the 8 and 26-28 kda b ... | 2003 | 12972729 |
| prevalence of intestinal parasite infections on a national scale among primary schoolchildren in laos. | in order to investigate the epidemiological situation of intestinal parasite infections in laos, parasitological surveys were carried out on a national scale including 17 provinces and the vientiane municipality. a total of 29,846 stool specimens were collected from primary schoolchildren from may 2000 to june 2002 and examined once with the cellophane thick smear technique. the cumulative egg positive rate for intestinal helminths was 61.9%. by species, the rate for ascaris lumbricoides was 34. ... | 2003 | 14574555 |
| [a case of severe eosinophilia in the acute phase of opisthorchis viverrini infection]. | a 26-year-old woman japanese public official servant in lao people's democratic republic was introduced to our hospital on december 12, 2002, because of two months duration of low grade fever and severe eosinophilia. there was no significant finding in physical examination. laboratory tests showed leukocytosis (15,700/microliter) with severe eosinophilia (42%), and no abnormal lymphocyte was observed. furthermore, elevation in alkaline phosphatase (748 iu/l), gamma-glutamyl transpeptidase (190 i ... | 2003 | 14574844 |
| detection of opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes by a pcr-based method. | a pcr procedure for the detection of opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes was developed. this procedure was based on primers designed from a pov-a6 specific probe sequence giving a 330 base-pair product. the detection was accomplished in host tissue homogenates to which a single cercaria or metacercaria was introduced. pcr can detect as little as a single cercaria artificially inoculated in a snail or a single metacercaria artificially inoculated ... | 2003 | 12613764 |
| experimental investigation of opisthorchiasis-associated cholangiocarcinoma induction in the syrian hamster - pointers for control of the human disease. | appropriate animal models for specific diseases in man can facilitate elucidation of mechanisms underlying tumour development and allow potential interventions and therapeutic regimens to be tested in vivo before consideration for use in the human situation. in the north-east of thailand exceptionally high levels of cholangiocellular carcinomas (cccs) are encountered, related to infestation with opisthorchis viverrini liver flukes. the syrian hamster can also be infected with metacercariae of th ... | 2003 | 12875618 |
| 8-nitroguanine formation in the liver of hamsters infected with opisthorchis viverrini. | nucleic acid damage by reactive nitrogen and oxygen species may contribute to the carcinogenesis associated with chronic infection and inflammation. we examined 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) formation and nitric oxide (no) production in hamsters infected with opisthorchis viverrini (ov). formation of 8-nitroguanine was assessed immunohistochemically with an antibody specific for 8-nitroguanine. 8-nitroguanine formation was found mainly in the cytoplasm and slig ... | 2003 | 12963027 |
| epidemiology of strongyloides stercoralis in north-east thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay. | cross-sectional surveys of parasitic infection were performed using the agar plate culture technique (apct) and modified formalin-ethyl acetate concentration technique (mfect) to assess the true prevalence of strongyloides stercoralis relative to other parasites in north-east thailand. the enzyme-linked immunosorbent assay (elisa) for diagnosis of s. stercoralis infection was used to estimate the seroprevalence for comparison with coproprevalence. faecal and serum samples were collected from stu ... | 2003 | 15259466 |
| opisthorchis viverrini and opisthorchiasis: the 21st century review. | 2003 | 14611870 | |
| opisthorchis viverrini and opisthorchiasis: a historical review and future perspective. | opisthorchiasis is caused by the liver fluke, opisthorchis viverrini. the fluke afflicts approximately seven million inhabitants in northeastern and northern thailand. the fluke utilizes, respectively, freshwater snails and cyprinoid fish as its first and second intermediate hosts. man is the accidental definitive host who acquires infection through the consumption of improperly cooked fish. information pertaining to the history, the life-cycle, the epidemiology and transmission dynamics, and di ... | 2003 | 14611871 |
| taxonomy and biology of liver flukes. | opisthorchis viverrini is a human liver fluke endemic in thailand, lao pdr and cambodia. three subspecies of bithynia siamensis and 18 species of cyprinoid fish are susceptible first and second intermediate hosts, respectively. the differentiation between the adult worm of o. viverrini and that of the closely related species, opisthorchis felineus and clonorchis sinensis, is mainly based on the shape and position of the testes and the arrangement of the vitellaria. eggs of these flukes are morph ... | 2003 | 14611872 |
| epidemiology of opisthorchis viverrini. | opisthorchis viverrini is a food borne trematode, important because of the sheer numbers of people infected and its serious morbidities such as hepatobiliary diseases and cholangiocarcinoma (chca). although infections are identified throughout southeast asia, the epi-center is northeast thailand, where high prevalence coexists with a high incidence of chca. in this review, we present the basic population features and the factors influencing transmission between the different hosts. | 2003 | 14611873 |
| immunology and molecular biology of opisthorchis viverrini infection. | opisthorchiasis is the major public health problems in laos pdr and thailand. the disease becomes chronic and persists for many years, leading to hepatobiliary disease and cholangiocarcinoma. less severe manifestations include cholangitis, chronic cholecystitis and cholelithiasis. a significant degree of humoral and cell mediated immune responses to the parasite can be detected both in patients and animal models. the patients igg levels appear to correlate with gall bladder size and dysfunction ... | 2003 | 14611874 |
| pathobiology of opisthorchiasis: an update. | opisthorchis viverrini infection is associated with several hepatobiliary diseases including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis and cholelithiasis. pathological consequences of o. viverrini infection occur mainly in the liver, extrahepatic bile ducts, gall bladder and kidney. these pathologies have been described in both humans and experimental animals. moreover, both experimental and epidemiological evidence strongly implicate the liver fluke infection in the etiolog ... | 2003 | 14611875 |
| comparative proteomic analysis of juvenile and adult liver fluke, opisthorchis viverrini. | we used comparative two-dimensional gel electrophoresis to highlight proteins that are differentially expressed in the maturation stage of the parasite opisthorchis viverrini (ov). the proteins differentially expressed in the juvenile/adult forms of the parasite are thought to be important for survival and pathogenesis. we used a nonlinear gradient ph ranged 3-10 strips for isoelectric focusing to resolve soluble proteins from four different maturation periods of ov from 1 week juvenile to 4 wee ... | 2003 | 14611878 |
| histological analysis of gallbladder diseases in relation to opisthorchiasis in endemic areas of thailand. | chronic gallbladder disease frequently accompanies infection with the liver fluke, opisthorchis viverrini, in northeast thailand. however, the pathology and pathogenesis of the gallbladder disease have not been described. accordingly, gallbladder specimens from 187 consecutive patients who had undergone cholecystectomy at a referral hospital in an endemic area in thailand were histologically characterized in relation to o. viverrini infection. the infection was assessed by the presence of parasi ... | 2003 | 14611879 |
| helminth parasites of cats from the vientiane province, laos, as indicators of the occurrence of causative agents of human parasitoses. | a total of 55 domestic cats (felis catus f. domestica) and one wild (bengal) cat (prionaluirus bengalensis) from the vientiane province, central laos, were examined for helminth parasites with emphasis given to potential human parasites. the following species were found (parasites infective to man marked with an asterisk): opisthorchis viverrini, haplorchis pumilio, h. taichui, h. yokogawai, stellantchasmus falcatus (digenea); spirometra sp., dipylidium caninum, taenia taeniaeformis (cestoda); c ... | 2003 | 14710631 |
| opisthorchis viverrini and opisthorchiasis: the 21st century review. proceedings of the congress of opisthorchiasis and cholangiocarcinoma. may 28-30, 2002. khon kaen, thailand. | 2003 | 14763464 | |
| intestinal parasitic infections in hill-tribe schoolchildren in chiang mai, northern thailand. | we surveyed intestinal parasitic infections in hill-tribe schoolchildren residing permanently in chiang mai province. the positive rate, of 403 stool specimens examined using the formalin-ether sedimentation technique, was 48.9%. no significant difference between male (50.8%) and female (47.1%) students was found for the infection. the most common protozoa was entamoeba coli (40.9%), followed by giardia lamblia (14.9%). the most common helminth was hookworm (13.4%), followed by ascaris lumbricoi ... | 2003 | 19230577 |
| studies on concomitant antigens of bithynia funiculata for detection of antibody to opisthorchis viverrini: effect of different centrifugal speeds on antigen preparation. | antigens derived from somatic extracts of bithynia funiculata, an intermediate snail host of o. viverrini, have been demonstrated to be highly heterogeneous in molecular weight (mw). these antigens have been suggested to be of potential use for serodiagnosis. in this study, b. funiculata somatic antigens were extracted using five different centrifugal speeds, namely 10,000 (c1); 20,000 (c2); 30,000 (c3); 40,000 (c4) and 50,000 (c5) rpm, with the aim of removing some non-specific antigens and det ... | 2003 | 19230581 |
| repeated infection with opisthorchis viverrini induces accumulation of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanine in the bile duct of hamsters via inducible nitric oxide synthase. | chronic inflammation induced by repeated infection with opisthorchis viverrini has been postulated to be a risk factor for cholangiocarcinoma. to clarify the mechanism of carcinogenesis induced by repeated o.viverrini infection, we investigated the timecourse of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) formation, inducible nitric oxide synthase (inos) expression, nitric oxide production and pathological features in hamsters with two (2-if) or three (3-if) o.viverrini infe ... | 2004 | 15059927 |
| prevalence of opisthorchis viverrini infection and incidence of cholangiocarcinoma in khon kaen, northeast thailand. | liver cancer is the most common cancer in khon kaen, northeast thailand, because of the high incidence of cholangiocarcinoma (chca). opisthorchis viverrini (ov), a liver fluke, is endemic in the area, and has been evaluated as a cause of chca by international agency for research on cancer. residents of 20 districts in the province were invited to attend a mobile screening programme between 1990 and 2001. of 24 723 participants, 18 393 aged 35-69 years were tested for ov infection, by examining s ... | 2004 | 15117303 |
| epidemiology of liver cancer: an overview. | the incidence of liver cancer varies widely throughout the world, with high rates in sub - saharan africa, eastern and southeastern asia, and melanesia and a low incidence in northern and western europe and the americas. primary cancers of the liver in adults are of two main histological types: hepatocellular carcinoma, which is derived from hepatocytes, and cholangiocarcinoma, which is derived from the epithelial lining of the intrahepatic bile ducts. hepatocellular cancer is a frequently occur ... | 2004 | 15244512 |
| epidemiological survey of intestinal parasitic infections of diarrhoeal patients in nepal and lao pdr. | intestinal parasitological survey was conducted to clarify the distribution of intestinal parasites in nepal and lao peoples' democratic republic (lao pdr) from 2001 to 2003. a total of 401 diarrheal fecal specimens were collected from patients aged one to 75 years (250 from nepal and 151 from lao pdr). the specimens were examined using the formalin-ether sedimentation (fes) and sucrose centrifugal flotation (scf) techniques. nine species (3 nematoda, 1 cestoda, and 5 protozoa) of parasites were ... | 2004 | 15449645 |
| changing patterns in intestinal parasitic infections among southeast asian laborers in taiwan. | in order to determine whether there are changing patterns in intestinal parasitic infections among foreign laborers in north taiwan, one fecal specimen was obtained from each of 1,569 laborers and examined by the formalin-ethyl acetate sedimentation technique. the prevalence of intestinal parasitic infections was 8.2%. females (11.7%) had a significantly higher prevalence than males (6.9%), although there was no significant difference between filipinos (7.7%), thais (8.3%) and indonesians (12.5% ... | 2004 | 14586642 |
| opisthorchiasis-associated biliary stones: light and scanning electron microscopic study. | biliary stones are frequently encountered in areas endemic for opisthorchiasis in thailand. the present study was to describe the prevalence and pathogenesis of these stones. | 2004 | 15484308 |
| hepatobiliary changes, antibody response, and alteration of liver enzymes in hamsters re-infected with opisthorchis viverrini. | we investigated pathological changes, antibody response, and liver enzymes in hamsters re-infected with opisthorchis viverrini. group 1 received a single dose of 50 metacercariae; groups 2 and 3 were first dosed with of 30 metacercariae and re-infected with 20 more once or twice at three month intervals. inflammation and liver cell necrosis were observed on 3d (day 3) for group 3 and 7d for group 2 in comparison with 21d for group 1. pathological changes included peri-ductal fibrosis, bile duct ... | 2004 | 15491546 |
| mechanism of no-mediated oxidative and nitrative dna damage in hamsters infected with opisthorchis viverrini: a model of inflammation-mediated carcinogenesis. | inflammation mediated by infection is an important factor causing carcinogenesis. opisthorchis viverrini (ov) infection is a risk factor of cholangiocarcinoma (chca), probably through chronic inflammation. formation of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg), and expression of inducible nitric oxide synthase (inos) and heme oxygenase-1 (ho-1) were assessed in the liver of hamsters infected with ov. we newly produced specific anti-8-nitroguanine antibody without cross-rea ... | 2004 | 15491850 |
| increased cell proliferation of mouse fibroblast nih-3t3 in vitro induced by excretory/secretory product(s) from opisthorchis viverrini. | infection by opisthorchis viverrini is a strong risk factor for cholangiocarcinoma. however, the mechanism by which the parasite is involved in carcinogenesis is not clear. in addition to the direct damage of the bile duct epithelium via direct contact with o. viverrini, the excretory/secretory (es) product(s) released from the parasites may play important roles in this process. we therefore investigated the responses of a fibroblast cell line, nih-3t3, to es product(s) released from o. viverrin ... | 2004 | 15521634 |
| the identification of a clonorchis sinensis gene encoding an antigenic egg protein. | the cdna library of clonorchis sinensis was screened for genes encoding antigenic proteins by using sera from clonorchiasis patients. a gene of 888 bp encoding a 28-kda protein (cs28) was cloned and found to contain a high percentage of glycine (20%), tyrosine (11%), and lysine (11%). the amino acid sequence of cs28 showed 60% homology with the vitelline b precursor protein of opisthorchis viverrini and of 33% homology with the vitelline b1 and b2 proteins of fasciola hepatica. a strong positive ... | 2004 | 15616856 |
| molecular cloning and characterization of a glutathione s-transferase encoding gene from opisthorchis viverrini. | an adult stage opisthorchis viverrini cdna library was constructed and screened for abundant transcripts. one of the isolated cdnas was found by sequence comparison to encode a glutathione s-transferase (gst) and was further analyzed for rna expression, encoded protein function, tissue distribution and cross-reactivity of the encoded protein with other trematode protein counterparts. the cdna has a size of 893 bp and encodes a gst of 213 amino acids length (ov28gst). the most closely-related gst ... | 2004 | 15783135 |
| detection of paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method. | a polymerase chain reaction (pcr) procedure for the detection of paragonimus heterotremus eggs in stool samples was developed and compared with stoll's egg count method. the primers were designed on the basis of a previously constructed pph-13-specific dna probe, which produced an approximate 0.5-kb amplified product. this pcr method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 x 10(-4) ng of p. heterotremus genomic dna. the a ... | 2005 | 15856902 |
| opisthorchis viverrini antigen induces the expression of toll-like receptor 2 in macrophage raw cell line. | opisthorchis viverrini infection induces inflammation in and around the bile duct, leading to cholangiocarcinoma in humans. to examine the mechanism of o. viverrini-induced inflammatory response, we assessed the expression of toll-like receptors (tlrs) in raw 264.7 macrophage cell line treated with an extract of o. viverrini antigen. flow cytometry and immunocytochemistry showed that o. viverrini antigen induced the expression of tlr2 but not tlr4. western blotting and immunocytochemistry reveal ... | 2005 | 15862573 |
| prevalence of strongyloides in northern thailand and treatment with ivermectin vs albendazole. | the stools of 697 cases were examined by agar plate technique at tambon makam luang, sun pa tong district, chiang mai; there were strongyloides stercoralis 15.9%,opisthorchis viverrini 5.1%, intestinal fluke 0.1%. treatment with ivermectin 78 cases and albendazole 33 cases of strongyloidiasis gave cure rates at 98.7% and 78.7%, respectively. alkaline phosphatase in some patients were increased at mild level after treatment. side effects in ivermectin group were anorexia, nausia, diarrhea, diffus ... | 2005 | 15916052 |
| association of parasitic infections and cancers. | recent advances in the fields of molecular biology, epidemiology and infectious diseases have led to significant revelations to clarify the relationship between cancer and infective agents. this article reviews the relationship between parasitic infections and carcinogenesis and the possible mechanisms involved. few parasites, e.g., schistosoma haematobium and opisthorchis viverrini have been found to be strongly associated with bladder cancer and cholangiocarcinoma respectively. the evidence fo ... | 2005 | 15928434 |
| establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (kku-100). | to establish and characterize a new cholangiocarcinoma cell line from a patient living in the opisthorchis viverrini (o. viverrini) endemic area of northeast thailand. | 2005 | 15948244 |
| genetic and environmental determinants of risk for cholangiocarcinoma via opisthorchis viverrini in a densely infested area in nakhon phanom, northeast thailand. | infection with opisthorchis viverrini (ov) is associated with cholangiocarcinoma. ov is common in northeast thailand, but less than 10% of the inhabitants develop cholangiocarcinoma. animal experiments suggest that ov infection alone does not cause cholangiocarcinoma, and thus other environmental and genetic factors may play a role in causation. we conducted a population-based case-control study in which sex, age and place of residence were matched individually. polymorphisms of gstm1 and gstt1 ... | 2005 | 15957169 |
| freshwater mollusks of medical importance in kalasin province, northeast thailand. | a snail survey was performed in six districts around irrigation areas of lampao dam, in kalasin province. the survey caught a total of 5,479 live snails and classed them into five families, 12 genera and 15 species, of which 7 species are suspected of transmitting human parasitic diseases. the seven species were pila polita, pomacea canaliculata, filopaludina (s.) m. martensi, bithynia (digoniostoma) siamensis goniomphalos, melanoides tuberculata, radix rubiginosa, and indoplanorbis exustus. of ... | 2005 | 16124433 |
| application of dipstick dye immunoassay (ddia) kit for the diagnosis of schistosomiasis mekongi. | the dipstick dye immunoassay (ddia), developed in china for the detection of antibodies against schistosoma japonicum, relies on soluble egg antigen (sea) labelled with a colloidal dye. this assay is not only rapid, simple and inexpensive, but also particularly useful for screening in the field. in order to determine whether s. japonicum antigens are sufficiently cross-reactive to make the assay applicable for the diagnosis also of s. mekongi a ddia approach based on the s. japonicum sea was tri ... | 2005 | 16143289 |
| mixed infections with opisthorchis viverrini and intestinal flukes in residents of vientiane municipality and saravane province in laos. | faecal examinations for helminth eggs were performed on 1869 people from two riverside localities, vientiane municipality and saravane province, along the mekong river, laos. to obtain adult flukes, 42 people positive for small trematode eggs (opisthorchis viverrini, heterophyid, or lecithodendriid eggs) were treated with a 20-30 mg kg(-1) single dose of praziquantel and purged. diarrhoeic stools were then collected from 36 people (18 in each area) and searched for helminth parasites using stere ... | 2005 | 16153322 |
| a prospective study of diet, lifestyle, and genetic factors and the risk of cancer in khon kaen province, northeast thailand: description of the cohort. | cohort studies are the preferred design in observational epidemiology, but few involving the general population have been performed in asia, and most concern affluent urban populations. the khon kaen study has recruited about 25,000 subjects, aged mainly 35-64, from villages in the relatively underdeveloped north-east of thailand. all subjects underwent simple physical examination, completed an interviewer-administered questionnaire (including sections on lifestyle, habits, and diet) and donated ... | 2005 | 16235989 |