Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| immunization of hamsters with tlck-killed parasites induces protection against leishmania infection. | hamsters immunized with n-p-tosyl-l-lysine-chloromethyl ketone tlck-treated l. brasiliensis brasiliensis (lb) from culture, infected with lb amastigotes presented: a gradual increase in t and b cell responsiveness to mitogens by lymph node lymphocytes, and an increased response to concanavalin a with no changes for dextran sulphate and pokeweed mitogen in splenocytes. absence of parasites in lymph nodes after 6 weeks post-infection and a nodule 4 times smaller than that of infected control anima ... | 1986 | 2877549 |
| topical pentostam in an attempt to produce more rapid healing of skin ulcers due to leishmania braziliensis braziliensis. | 1986 | 2825253 | |
| leishmania donovani: cellular and humoral immune responses after primary and challenge infections in squirrel monkeys, saimiri sciureus. | cellular and humoral immune responses were studied in squirrel monkeys after primary and challenge infection with a khartoum strain (wr 378) of leishmania donovani. each of 7 squirrel monkeys, saimiri sciureus, was inoculated intravenously with 5 x 10(7) amastigotes/kg body weight, and one other monkey (control) was inoculated with uninfected hamster spleen homogenate. five infected monkeys recovered from visceral leishmaniasis and two infected monkeys died. three of the five squirrel monkeys wh ... | 1986 | 2940111 |
| do sugar residues contribute to the antigenic determinants responsible for protection and/or abolition of protection in leishmania-infected balb/c mice? | intravenous inoculation of irradiated virulent promastigotes of leishmania mexicana, or intradermal inoculation of avirulent temperature-sensitive clones of the same parasite, can protect balb/c mice from progressive disease when challenged with parental organisms. however, if animals are prechallenged s.c. with irradiated parental parasites before (or shortly after, i.e., within 10 days) any other immunization regime is used, the s.c. challenge effectively suppresses development of protective i ... | 1986 | 2424979 |
| comparative studies of leishmania soluble antigens by crossed-immunoelectrophoresis: demonstration of specific antigens to l. mexicana and l. braziliensis. | the antigenic relationship between two species of leishmania (l. mexicana amazonensis and l. braziliensis braziliensis) was analysed using crossed-immunoelectrophoresis with an intermediate gel. rabbit hyperimmune sera were prepared by subcutaneous inoculations of soluble antigens obtained from each species, emulsified in freund's adjuvant. the antisera were then tested by immunodiffusion and electrophoresis; soluble antigens obtained from promastigote forms were submitted to ultrasonication and ... | 1986 | 2425997 |
| antibodies to basement membrane protein nidogen in chagas' disease and american cutaneous leishmaniasis. | about 50 to 70% of sera from patients with american cutaneous leishmaniasis and chronic chagas' disease possessed antibodies which reacted in enzyme and radioimmunoassays with nidogen obtained from a tumor basement membrane. the antibodies were of the immunoglobulin m and g classes in acute american cutaneous leishmaniasis but mainly of the immunoglobulin g class in chronic chagas' disease. similar antibodies could not be detected in patients suffering from a variety of other infectious or infla ... | 1986 | 2429987 |
| characterization and quantitation of membrane antigens of new world leishmania species by using monoclonal antibodies in western blot and flow microfluorometric assays. | membrane-specific monoclonal antibodies generated against promastigotes of new world leishmania species were used in western blot, elisa, and flow microfluorometric assays to characterize their antigen specificity and to determine the external surface distribution of the reactive epitopes. three major membrane antigens of molecular weight 72 kd, 55 kd, and 42 kd were identified as well as a dominant antigen that migrated as a broad band on sds-page, corresponding to a molecular weight of 10-15 k ... | 1986 | 2432268 |
| identification and recovery of leishmania antigen displayed on the surface membrane of mouse peritoneal macrophages infected in vitro. | a murine monoclonal antibody (83l-2d3/a) to a dominant surface antigen of leishmania braziliensis panamensis (wrair-470) recognized a determinant expressed on the surface membrane of mouse peritoneal macrophages infected in vitro. this determinant, also demonstrable on the surface membrane of intracellular amastigotes, was not displayed by the macrophage until at least 6 hr post-infection. this delay in expression and the obvious negativity of all uninfected macrophages inherent to infected cult ... | 1986 | 3950404 |
| karyotype analysis of leishmania species and its use in classification and clinical diagnosis. | chromosomes of four species of leishmania represented by ten different geographic isolates were analyzed by pulsed field gradient gel electrophoresis (pfg) to assess chromosome stability in these parasitic protozoans. among different geographic isolates of the same subspecies, more than two-thirds of chromosomes had similar sizes, ethidium bromide staining intensities, and locations of alpha,beta-tubulin genes. however, among new world leishmania, members of different species or subspecies have ... | 1986 | 3961502 |
| [note on the relations between vectors of leishmaniasis and forest trees in french guiana]. | the study of relationships between phlebotomine sandflies and different species of trees, 0 to 1.5 m above ground, was carried out in the french guiana forest. we found that certain forest trees are more propitious than others to the development or maintenance of certain leishmaniasis vectors. lu. umbratilis, vector of leishmania braziliensis guyanensis, represents between 28 and 88% of samples collected on trunks. the association of lu. umbratilis and lu. rorotaensis represents between 58 and 9 ... | 1986 | 3813430 |
| [leishmania braziliensis vianna, 1911 s. st., in french guiana]. | 1986 | 3813437 | |
| disseminated cutaneous leishmaniasis in a field clinic in bahia, brazil: a report of eight cases. | eight bahian patients with cutaneous leishmaniasis who had 20 or more ulcerative lesions of short duration are described. of five identifications of isolated parasites, four were leishmania braziliensis braziliensis and one was l. mexicana amazonensis. all but one had positive montenegro tests initially, and all did after treatment. all had circulating anti-leishmanial antibodies and five responded well to glucantime therapy suggesting a functioning immune response. this is quite different to th ... | 1986 | 3806749 |
| biochemical identities and differences among leishmania species and subspecies. | an analysis was presented for identification of 20 species and subspecies of leishmania by cellulose acetate electrophoresis data from the enzymes glucose phosphate isomerase, mannose phosphate isomerase, and phosphogluconate dehydrogenase. most leishmania could be identified from data of these three enzymes. the cae data for 20 enzymes from over 300 new and old world isolates were combined, and an analysis of the data which included calculations of genetic identities and genetic distances was r ... | 1987 | 3812883 |
| [changes in immunologic and biochemical functions after a mission to a tropical forest]. | a biochemical and immunological survey of a selected military group has been performed, before and after a jungle raid. biochemical (total proteins, albumin, proteins electrophoresis, transferrin, haptoglobin, ceruloplasmin, orosomucoid, alpha-1 antitrypsin and alpha-2 macroglobin) and immunological (ig g., ig a., c 3c, c 4, specific antibodies against leishmania) analysis of sera have been studied as well as the level of cellular immunity (multitest scoring, macrophage cytotoxicity, interleukin ... | 1987 | 3431382 |
| correlation of sinefungin susceptibility and drug-affinity for protein carboxymethyltransferase activity in american leishmania species. | among promastigotes of 22 different american leishmania strains, a 5000-fold variation in sinefungin susceptibility was found, apparently independent of their taxonomic classification, although l. mexicana strains did tend to be more resistant than l. braziliensis. protein carboxymethyltransferase (ec 2.1.1.24) and glycine n-methyltransferase (ec 2.1.1.20) activities were not substantially different in sinefungin-susceptible and -resistant american leishmania strains. however, when [methyl-3h]me ... | 1987 | 3431568 |
| identification and distribution of new world leishmania species characterized by serodeme analysis using monoclonal antibodies. | five hundred thirty stocks of leishmania isolated from human and domestic and wild reservoir hosts, representing a wide geographic distribution of endemic foci of american cutaneous (acl) and visceral leishmaniases (avl) were characterized and identified at species and/or subspecies levels based on their reactivity to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. this study confirms and extends our preliminary results on the high specificity of some of these ... | 1987 | 3826486 |
| diagnosis of cutaneous and mucocutaneous leishmaniasis in colombia: a comparison of seven methods. | seven methods of diagnosing leishmaniasis were compared in 177 patients presenting with lesions of the skin (165) or mucosa (12) in tumaco and cali, colombia. the three methods of visualizing amastigotes in tissue samples (histological staining of tissue sections, impression smears of punch biopsies, and smears of dermal scraping from slits in the lesion margins) were less sensitive than the four leishmania isolation methods (aspiration of lesion border cultured in biphasic media, aspirate inocu ... | 1987 | 2437815 |
| leishmaniasis in brazil. xxv. sandfly vectors of leishmania in pará state, brazil. | leishmania of the braziliensis complex were isolated from various members of the squamiventris series sandflies, including psychodopygus chagasi (costa lima), p.s.maripaensis (ready et al.), p.s.squamiventris (lutz & neiva), and also p.ayrozai (barreto & coutinho) and lutzomyia umbratilis ward & fraiha. three different serodemes of le.b.guyanensis floch were isolated from lu.anduzei rozeboom, lu.umbratilis and lu.whitmani antunes & coutinho, simultaneously captured in the same area. unidentified ... | 1987 | 2979555 |
| oxidation of glucose, ribose, alanine, and glutamate by leishmania braziliensis panamensis. | the metabolism of [1-14c]- and [6-14c]glucose, [1-14c]ribose, [1-14c]- and [u-14c]alanine, and [1-14c]- and [5-14c]glutamate by the promastigotes of leishmania braziliensis panamensis was investigated in cells resuspended in hanks' balanced salt solution supplemented with ribose, alanine, or glutamate. the ratio of 14co2 produced from [1-14c]glucose to that from [6-14c]glucose ranged from about two to six, indicating appreciable carbon flow through the pentose phosphate pathway. a functional pen ... | 1987 | 2884307 |
| safety and efficacy of high-dose sodium stibogluconate therapy of american cutaneous leishmaniasis. | 40 patients with american cutaneous leishmaniasis caused primarily by leishmania braziliensis panamensis were treated with sodium stibogluconate in a double-blind, randomised controlled trial. nine weeks after starting treatment, all 19 patients treated with 20 mg sb/kg per day for twenty days were cured but 5 of 21 patients treated with 10 mg sb/kg per day for twenty days had persistent active disease (p less than 0.05). both treatment regimens were well tolerated and they were associated with ... | 1987 | 2885505 |
| leishmania spp.: agar plating as an alternative to limiting dilution and impression smears for the enumeration of viable parasites in tissue. | 1987 | 3803532 | |
| sporotrichoid leishmaniasis: report of a case in an american graduate student. | 1987 | 3680670 | |
| leishmaniasis in brazil. xxiv. natural flagellate infections of sandflies (diptera: psychodidae) in pará state, with particular reference to the rôle of psychodopygus wellcomei as the vector of leishmania braziliensis braziliensis in the serra dos carajás. | between july 1983 and december 1984 natural flagellate infections were found in 114 (1%) of 11,586 female phlebotomine sandflies (diptera: psychodidae) of 21 species. a further 1084 females of 17 other species were not infected. identification of the organisms on a number of occasions confirms the exclusive parasite/vector relationship of leishmania mexicana amazonensis/lutzomyia flaviscutellata and le. braziliensis braziliensis/psychodopygus "wellcomei". undescribed or unidentified leishmania s ... | 1987 | 3686628 |
| detection and enumeration of leishmania in sand flies using agar-based media. | an agar plating technique was used to determine the number of amastigotes ingested by lutzomyia longipalpis fed on papules on mesocricetus auratus caused by leishmania mexicana amazonensis and on lesions on mystromys albicaudatus caused by leishmania braziliensis panamensis. the technique involved homogenizing sand flies after bloodfeeding on the infected animals and spreading the homogenate over the surface of agar plates. a great variation in the number of amastigotes ingested by individual sa ... | 1987 | 3688307 |
| infectivity of leishmania braziliensis promastigotes is dependent on the increasing expression of a 65,000-dalton surface antigen. | sequential development of leishmania braziliensis promastigotes from a noninfective to an infective stage was demonstrated. the generation of infective forms was related to their growth cycle and restricted to stationary stage organisms. using immunofluorescence techniques, we have noticed that the binding of a monoclonal antibody (mab) against l. braziliensis (vd5/25) increased progressively as the promastigotes developed in culture and was maximal with the infective forms. this antigenic diffe ... | 1987 | 3782801 |
| isoenzyme studies on leishmania stocks from peru by ultrathin-layer isoelectrofocusing. | 17 leishmania stocks isolated from the andean and amazonean region of peru were compared isoenzymatically with reference stocks of new world leishmania subspecies by ultra-thin-layer isoelectric focusing. the enzymes tested were: non-specific esterase (nse), alcohol dehydrogenase (adh) (nadp+), glucosephosphate isomerase (gpi) and superoxide dismutases (sod). two enzymes, gpi and sod, gave a promising tool for differentiation of the majority of subspecies. these enzymes classify the stocks from ... | 1987 | 3602838 |
| experimental american leishmaniasis in the brazilian squirrel monkey (saimiri sciureus): lesions, hematology, cellular, and humoral immune responses. | unulcerated cutaneous lesions appeared and persisted in squirrel monkeys experimentally infected with leishmania braziliensis braziliensis or l. b. panamensis. peripheral blood mononuclear cell (pbmc) numbers increased following infection, and cultured pbmcs from infected monkeys proliferated in response to parasite antigens. the responses of pbmcs to mitogens were not suppressed in infected monkeys. elevated levels of leishmania-specific immunoglobulins m and g were also observed. thus, the squ ... | 1987 | 3612761 |
| american leishmania spp: formycin b treatment of cutaneous leishmaniasis in mice. | using foot-pad infection of female c57bl/6, dba/2j and nmri-ivic mice as an animal model for american cutaneous leishmaniasis (acl), we evaluated the inhibitory effect of formycin b (fob) on the infection produced by 7 different leishmania isolates. when treatment was initiated some days, or even some weeks, after infection a significant leishmanistatic effect was detected on mice infected with all leishmania isolates, which reached 30-55 weeks for some isolates. the optimal dose schedule was 1. ... | 1987 | 3614989 |
| identification of a major 72 kilodalton surface antigen in twelve isolates of leishmania braziliensis braziliensis. | the study of the surface antigens of leishmania braziliensis braziliensis revealed a great homogeneity among ten strains isolated from bolivia and two reference strains from brazil and belize. a 72 kda major protein, present in all l. b. braziliensis strains, was recognized by both cutaneous and mucocutaneous human sera, but was not recognized by kala-azar and chagasic sera. no cross-reactive antigens were found among strains of leishmania braziliensis guyanensis, leishmania braziliensis panamen ... | 1987 | 3627165 |
| isolations of leishmania braziliensis (kinetoplastida: trypanosomatidae) from cryopreserved colombian sand flies (diptera: psychodidae) | 1987 | 3669032 | |
| the effect of tunicamycin on leishmania brasiliensis. glycosylation and the cell surface components. | culture conditions of leishmania cells were developed to allow the study of the effect of tunicamycin (tm) on glycosylation and on the cell surface components. leishmania incorporate [14c]-mannose and [35s]-methionine in vitro. the incorporation of [14c]-mannose is linear for 150 min and is inhibited by tm (2 micrograms/ml) in a time dependent effect which reaches a plateau of 45% inhibition at 36 h. under the same experimental conditions [35s]-methionine incorporation into protein is slightly a ... | 1987 | 3670285 |
| epidemiology of american cutaneous leishmaniasis due to leishmania braziliensis braziliensis. | a five-year prospective study of cutaneous leishmaniasis in an endemic area of brazil revealed an annual incidence of disease of 8.1 per 1000 inhabitants and a prevalence of 14.9%. the disease fluctuated as a series of mini-epidemics. most disease occurred in individuals who were 10-30 years of age. mucosal disease occurred in 2.7% of patients with primary lesions and occurred a median of six years after this lesion. disease was more common in males, in those with either large or multiple antece ... | 1987 | 3598227 |
| leishmania braziliensis: protein, carbohydrate, and antigen differences between log phase and stationary phase promastigotes in vitro. | when leishmania species are grown in vitro, parasites from the stationary phase differ from those in log phase growth in being more infective and more resistant to complement and macrophage mediated killing. in the present study, log phase and stationary phase promastigotes of leishmania braziliensis panamensis were compared at the molecular level. differences in polypeptide and glycoprotein composition and antigenicity between log and stationary phase promastigotes of l. b. panamensis were dete ... | 1987 | 3582573 |
| antibody responses, as measured by the enzyme-linked immunosorbent assay (elisa), in owl monkeys experimentally infected with leishmania braziliensis panamensis. | 1987 | 3585642 | |
| in vitro reversible transformation of leishmania braziliensis panamensis between promastigote and ellipsoidal forms. | raising the temperature of a log-phase culture of leishmania braziliensis panamensis promastigotes from 26 degrees c to 34 degrees c resulted in formation of a culture containing 85% ellipsoidally shaped forms after 1.5 h. the temperature-induced ellipsoidal forms decreased in size but persisted in high proportion (85-95%) for at least 12 h at 34 degrees c. recovery from the ellipsoidal forms to a culture containing 85-95% promastigotes was observed after returning the temperature to 26 degrees ... | 1987 | 3585815 |
| oxidation of fatty acids by leishmania braziliensis panamensis. | heating cultures of leishmania braziliensis panamensis (grown at 26 degrees c) to 34 degrees c for 1.5-12 h transformed the cells to an ellipsoidally shaped form. the heat treatment caused an increase in the rate of oxidation of both medium and long chain fatty acids but decreased the rate of oxidation of [1-14c]glucose. the rate of fatty acid oxidation continued to increase for times as long as 20 h after returning the cultures to 26 degrees c. in both the promastigote and heat-induced ellipsoi ... | 1987 | 3585816 |
| serodiagnosis of new world leishmaniasis by using a genus-specific antigen in enzyme linked immunosorbent assays. | a genus-specific monoclonal antibody (83l-5g9), generated against promastigotes of leishmania braziliensis panamensis (wrair-470), has been used as a ligand in immunochromatography columns for the recovery and purification of the genus-specific antigen. when the purified polypeptide, actually a doublet comprised of a 58 kd and a 31 kd moiety, was used as the antigen in enzyme linked immunosorbent assays (elisa), it was reactive with 36 of 85 sera from leishmania patients and with 1 of 49 sera fr ... | 1987 | 3552391 |
| topical treatment of new and old world cutaneous leishmaniasis in experimental animals. | the effect of topical treatment with 15% paromomycin sulphate and 12% methylbenzethonium chloride in white soft paraffin on cutaneous leishmaniasis in balb/c mice was studied. the saudi arabian strain of leishmania major, although being the most virulent to the mice, showed the highest susceptibility to this treatment. after 10 days treatment, parasites were totally eliminated from the treated lesion and healing was complete. 3 american strains were also tested: l. mexicana amazonensis; l. brazi ... | 1987 | 3449988 |
| cutaneous leishmaniasis in bolivia. a study of 185 human cases from alto beni (la paz department). isolation and isoenzyme characterization of 26 strains of leishmania braziliensis braziliensis [corrected]. | a clinical, serological, parasitological and therapeutic study of cutaneous leishmaniasis was carried out in a low sub-andean area (250-800 metres) of the la paz department, bolivia. a team of seismic prospectors (350 workers) was surveyed for 12 months. of 200 suspected cases of cutaneous leishmaniasis, 185 were serologically or parasitologically confirmed (incidence 52.8%). those exposed to the greatest risk of infection were working in a virgin forest environment. leishmanial organisms were i ... | 1987 | 3449990 |
| isolation and characterization of calmodulin from leishmania braziliensis and leishmania mexicana. | 1987 | 3453576 | |
| products of leishmania braziliensis glucose catabolism: release of d-lactate and, under anaerobic conditions, glycerol. | leishmania braziliensis panamensis promastigotes were incubated with glucose as the sole carbon source. about one-fifth of the glucose consumed under aerobic conditions was oxidized to co2. nuclear magnetic resonance studies with [1-13c]glucose showed that the other products released were succinate, acetate, alanine, pyruvate, and lactate. under anaerobic conditions, lactate output increased, glycerol became a major product, and, surprisingly, glucose consumption decreased. enzymatic assays show ... | 1987 | 3478686 |
| dermal leishmaniasis in the amazon region of brazil: leishmania (viannaia) lainsoni sp.n., a new parasite from the state of pará. | 1987 | 3506634 | |
| human, canine and equine leishmaniasis caused by leishmania braziliensis braziliensis in an endemic area in the state of rio de janeiro. | 1987 | 3507562 | |
| cutaneous leishmaniasis in a horse (equus caballus) from endemic area in the state of espirito santo, brazil. | 1987 | 3507577 | |
| disseminated american cutaneous leishmaniasis in a patient with aids. | 1987 | 3507921 | |
| characterization of leishmania species from peru. | twenty-six isolates of leishmania parasites of peruvian origin were studied by isoenzyme electrophoresis of four marker enzymes (asat, alat, g6pd and gpi), kinetoplast dna hybridization and monoclonal antibody binding and compared with marker strains of the new world organisms l. b. braziliensis, l. b. guyanensis, l. m. mexicana and l. m. amazonensis. 12 of the isolates studied were of andean origin; 11 of these were isolated from patients with andean cutaneous leishmaniasis. the organisms origi ... | 1987 | 3445300 |
| leishmaniasis in brazil: xxiii. the identification of leishmania braziliensis braziliensis in wild-caught neotropical sandflies using monoclonal antibodies. | using the indirect immunofluorescence test natural flagellate infections of wild-caught sandflies, from the serra dos carajás region of pará state, brazil, were identified by sequentially staining smears made from the infected flies with monoclonal antibodies. with normal methods of isolation 30% of the infections were identifiable, but when monoclonal antibodies specific to leishmania braziliensis braziliensis were used a further 26% were identified. the staining of organisms in smears of natur ... | 1987 | 3445325 |
| spontaneous healing of cutaneous leishmania braziliensis braziliensis ulcers. | 1987 | 3445344 | |
| identification of the promastigote surface protease in seven species of leishmania. | twelve different strains of leishmania, including l. major, l. donovani, l. infantum, l. tropica, l. mexicana, l. amazonensis, l. braziliensis, and l. enriettii were examined for the presence of an ectoenzyme structurally and functionally related to the promastigote surface protease found in l. major lem 513. all strains examined possess a protease that is labelled by surface iodination of living promastigotes. the electrophoretic migrations of the labelled proteases are similar in all species s ... | 1987 | 3302702 |
| macrophage function in patients with american cutaneous leishmaniasis: in vitro cytotoxicity and interleukin-1 production. | monocyte-derived blood macrophages of untreated patients with leishmania braziliensis or leishmania mexicana amazonensis infections show anomalies in their nonspecific immune functions. their ability to kill hela cells or to produce interleukin-1 in vitro in response to lipopolysaccharide plus candida albicans is lower than controls indicating that acquired or innate macrophage deficiencies may be involved in the course of the disease. | 1987 | 3305852 |
| [advances and perspectives in american cutaneous leishmaniasis]. | the advances and perspectives in american (mucocutaneous) leishmaniasis are presented under the following topics: 1. epidemiology in latin america, particularly in brazil. 2. parameters for taxonomic identification of species and subspecies of the genus leishmania, which belong to l. mexicana and l. braziliensis complexes: biologic behaviour in hamster, in culture, in animal reservoir, clinical aspects, vectors, electronic microscopy, electrophoretic mobility of isoenzymes, radiorespirometry, dn ... | 1987 | 3309496 |
| [leishmaniasis of the genital organs]. | five cases of leishmaniasis in genital organs are exposed. the patients are males, farmers, with less than 30 years old (except one), residents of high leishmaniasis incidence zones. the majority of lesions were in penis, and of scabies ulcer type, destructive, painless and of slow evolution. in this cases generally, the clinic diagnosis was difficult to corroborate by the habitual laboratory methods and we insist that the principal differential diagnosis are the neoplastic process of the zone. ... | 1987 | 3309500 |
| generation of leishmania-specific short-term t-cell lines derived from peripheral blood of cured american cutaneous leishmaniasis patients. | the putative protective immune response in localized cutaneous leishmaniasis caused by leishmania braziliensis braziliensis (lbb) was analysed by generation of short term t-cell lines from peripheral blood lymphocytes (pbl) of three patients. all had had active skin lesions but were clinically cured at the time of study. pbl were stimulated in vitro with sonicated lbb promastigotes; blasts were isolated after 5 days and propagated for 9 to 16 days before being tested for proliferative capacity. ... | 1987 | 3314910 |
| [effects of clofazimine on leishmania promastigotes]. | 1987 | 3321231 | |
| the ribosomal gene spacer as a tool for the taxonomy of leishmania. | a recombinant dna ribosomal gene spacer of leishmania braziliensis y was used as probe to test different leishmania species. based on the similarity of their restriction patterns, three groups were distinguished with respect to international leishmania references: first a group with a similar restriction pattern to l. braziliensis y and the reference organism l. mexicana garnhami jap78; a second group with restriction patterns similar to the reference organism l. mexicana mexicana m379; and fina ... | 1987 | 3033494 |
| detection of leishmania parasites by dna in situ hybridization with non-radioactive probes. | in situ hybridization techniques develop rapidly into diagnostic tools of considerable value for detection of viruses and bacteria. here we report the application of this technique for the detection of leishmania parasites. biotin-labelled total promastigote dna was hybridized to cultured leishmania parasites and to blood and impression smears of infected mice. in promastigotes kinetoplasts were strongly stained, nuclei somewhat more diffuse. in amastigotes both nuclear and kinetoplast dna hybri ... | 1987 | 3035535 |
| a comparative study of d-lactate, l-lactate and glycerol formation by four species of leishmania and by trypanosoma lewisi and trypanosoma brucei gambiense. | leishmania braziliensis panamensis, l. donovani, l. major, and l. mexicana amazonensis promastigotes, trypanosoma lewisi bloodstream forms, and t. brucei gambiense procyclic forms were incubated with glucose as sole carbon source. all species consumed glucose more rapidly under aerobic than anaerobic conditions. all produced glycerol under anaerobic conditions, though the rate of glycerol production by t. lewisi was markedly lower than that by the other species. the four leishmania species produ ... | 1988 | 3054535 |
| growth curves of leishmania braziliensis braziliensis promastigotes and surface antigen expression before and after adaptation to schneider's drosophila medium as assessed by anti-leishmania human sera. | 1988 | 3059439 | |
| [observations on kala azar in jacobina, bahia. vi. investigations on sylvatic and commensal mammals]. | 1988 | 3068712 | |
| indirect immunofluorescence test in new world leishmaniasis: serological and clinical relationship. | the indirect immunofluorescence test (if) for anti-leishmania antibodies (igg and igm) was performed with sera from the following groups of individuals: 214 cutaneous leishmaniasis patients, 28 healthy subjects with positive montenegro's skin test (mst), 29 healthy subjects with negative mst and 16 visceral leishmaniasis patients. the first four groups came from a suburban area of rio de janeiro (jacarepaguá) where cutaneous leishmaniasis caused by leishmania braziliensis braziliensis is endemic ... | 1988 | 3078345 |
| leishmania braziliensis panamensis: increased infectivity resulting from heat shock. | promastigotes of leishmania braziliensis panamensis were subjected to a heat shock transformation yielding an amastigote-like stage. during the process of conversion, the heat-induced differentiating form displayed an increase in infectivity (as determined by lesion size) accompanied by a total protein composition unlike that of the promastigote and a morphology resembling that of the amastigote. these biological/functional changes may be related to an involvement of a heat shock response in the ... | 1988 | 3338542 |
| cutaneous leishmaniasis in guatemala: isoenzyme characterization of isolates from humans. | leishmania organisms cultivated from cutaneous lesions of humans in guatemala were characterized by cellulose acetate electrophoresis. six isolates had electrophoretic enzyme patterns identical to world health organization reference strains of leishmania braziliensis braziliensis, and 5 had patterns identical to reference strains of leishmania mexicana mexicana. | 1988 | 3341522 |
| the use of nonradioactive dna probes for the characterization of leishmania isolates from peru. | we describe conditions for dot blot dna hybridization studies using biotinylated kdna probes from leishmania. the sensitivity and specificity attained with biotinylated or 32p-labeled probes were equivalent. the lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with proteinase k. studies were performed with 112 leishmania isolates from andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the ... | 1988 | 3354765 |
| recovery of leishmania (viannia) braziliensis from inoculated hamsters. | leishmania (viannia) braziliensis has never been isolated from wild animals although it is apparently capable of inducing infections in man, dogs, and donkeys. an analysis of the standard hamster culture system for analyzing infectivity of leishmania sp. was undertaken. results indicate that for l. (v.) braziliensis, routine cultivation of aspirates taken from the inoculation sites of 1-mo-infected hamsters should be undertaken. moreover, in at least 1 of the 3 strains examined, isolation of the ... | 1988 | 3379536 |
| the identification of membrane glycoconjugates in leishmania species. | the membrane glycoconjugates of 8 different species of leishmania were compared by lectin blotting. five different lectins with various sugar specificities were examined: concanavalin a, lens culinaris, ricinus communis, soybean agglutinin, and peanut agglutinin. concanavalin a and lens culinaris reacted with every leishmania tested. the patterns observed for these 2 lectins, as well as the various species of parasites, were different. however, a common 41,000-52,000 and a 160,000-185,000 mr com ... | 1988 | 3397816 |
| genetic characterization of leishmania isolates at 37 enzyme loci. | 1988 | 3417374 | |
| d-lactate production by leishmania braziliensis through the glyoxalase pathway. | leishmania braziliensis promastigotes incubated anaerobically produce d-lactate from glucose, ribose, and methylglyoxal, but not from glycerol, alanine, or pyruvate, suggesting the presence of glyoxalases i and ii but the absence of d-lactate dehydrogenase. further support for this is shown by: (1) conversion of methylglyoxal to d-lactate in sonicates of promastigotes in the presence of reduced glutathione, (2) utilization of phenylglyoxal at rates comparable to methylglyoxal, (3) lack of utiliz ... | 1988 | 3130573 |
| isolation of leishmania from monkeys in the amazon region of brazil. | 1988 | 3142112 | |
| experimental american leishmaniasis and chagas' disease in the brazilian squirrel monkey: cross immunity and electrocardiographic studies of monkeys infected with leishmania braziliensis and trypanosoma cruzi. | 1988 | 3146554 | |
| differential growth requirements of several leishmania spp. in chemically defined culture media. | 17 strains of leishmania from 4 species: brasiliensis, mexicana, donovani and garnhami have been continually cultured at 26 degrees c, in the absence of proteins, in a medium containing salts, glucose, d-ribose, 2-deoxyribose, hemin, tricine, hepes, 34 amino acids and intermediates of amino acid metabolism, 23 vitamins, 6 nucleotides and tetrahydrofolic acid. a wide variation in growth requirements was observed among leishmaniae which permitted the preparation of different minimum culture media ... | 1988 | 2901198 |
| requirements of defined cultivation conditions for standard growth of leishmania promastigotes in vitro. | the growth characteristics of l. chagasi (mhom/br/79/li01) and l. braziliensis (mhom/br/72/1670), the causative agents of visceral and muco-cutaneous leishmaniases, respectively, were compared. inoculum size clearly influences the growth course of both leishmania species, whatever the culture medium used (serum-supplemented media: glsh or rpmi, and a chemically defined medium: litr9). cultures initiated with low concentrations failed to promote cell growth, while typical growth curves were obtai ... | 1988 | 2901211 |
| a tissue culture system for the growth of several species of leishmania: growth kinetics and drug sensitivities. | we have developed a simple in vitro method of infecting a continuous human macrophage cell line (u937) with promastigotes of several species of leishmania. these include l. braziliensis braziliensis, l. b. panamensis, l. donovani, l. mexicana mexicana, l. m. pifanoi, l. tropica, and l. major. the growth kinetics of these species are presented as well as drug sensitivity data. the u937 cell system can be used to determine drug efficacy and eliminates the need to use amastigotes from animal tissue ... | 1988 | 2833127 |
| the synergistic action of pyrazolopyrimidines and pentavalent antimony against leishmania donovani and l. braziliensis. | pyrazolopyrimidines, particularly allopurinol, allopurinol riboside, and other purine analogues, show promise as experimental therapeutic compounds for the treatment of leishmaniasis. the combination of these agents with pentostam may produce an improved therapeutic effect. we report here on strong synergistic activity between pyrazolopyrimidines and pentavalent antimonials in a human macrophage tissue culture system infected with leishmania donovani and l. braziliensis. | 1988 | 2845824 |
| effects of antimycotic azoles on growth and sterol biosynthesis of leishmania promastigotes. | promastigotes of 36 world health organization reference (and other) strains of 6 species and 10 subspecies of leishmania were cultured in the presence of 3 antimycotic azole drugs (ketoconazole, itraconazole, fluconazole) and their population growth determined. a representative of each subspecies was also analyzed for its sterol composition. for all strains the order of azole drug activity with respect to both growth and sterol biosynthesis inhibition was itraconazole greater than or equal to ke ... | 1988 | 2847043 |
| detection of leishmania within sand flies by kinetoplast dna hybridization. | a kinetoplast dna hybridization probe method was used to detect leishmania within sand flies and to distinguish it from the non-pathogenic flagellate, endotrypanum. eighty-one sand flies (74 lutzomyia umbratilis, 1 lu. anduzei, and 6 lu. shannoni) collected outside manaus, brazil were dissected. forty-four of these were found to be infected with flagellates, and 2 hybridized with a leishmania braziliensis probe. thirty-three of sixty-one flies reprobed with an endotrypanum probe were positive. | 1988 | 2848422 |
| [leishmaniasis caused by leishmania viannia braziliensis (lvb). a case of atypical course]. | 1988 | 2854901 | |
| primary agent for leishmaniasis. | 1988 | 2856321 | |
| leishmania braziliensis braziliensis isolated from akodon arviculoides captured in caratinga, minas gerais, brazil. | 1988 | 3176154 | |
| leishmaniasis in colombia. i. studies on the phlebotomine fauna associated with endemic foci in the pacific coast region. | studies on the phlebotomine fauna related to the leishmaniasis endemic foci of the colombian pacific coast were carried out in the municipalities of tumaco and buenaventura. in inguapí del guadual, tumaco, lutzomyia trapidoi and lu. gomezi were the predominant anthropophilic species; lu. panamensis and lu. hartmanni were less frequent. in bajo calima, buenaventura, lu. trapidoi represented over 94% of the anthropophilic sandflies. continuous sampling from 1800 to 0600 hours in inguapí del guadua ... | 1988 | 3177739 |
| acid phosphatase isoenzyme mapping in leishmania. | the profiles of acid phosphatase isoenzymes of several well defined species of the genus leishmania were compared. the profiles were generated after isoelectric focusing of parasite extracts in polyacrylamide and incubation of the gels with an appropriate substrate coupled to an azo dye. analysis of the zymograms showed that there is species-specificity of the acid phosphatase isoenzyme maps in leishmania. it was also demonstrated that different strains of the same species present identical patt ... | 1988 | 3191957 |
| behavior of leishmania braziliensis s.l. in golden hamsters: evolution of the infection under different experimental conditions. | reproducibility of leishmania braziliensis s.l. metastatic behavior in hamsters was studied for 9 isolates of l.b. panamensis and 2 of l.b. guyanensis with a previous record of metastasis. also, the influence of corticosteroids and trauma was evaluated. in the corticosteroid-treated group, metastases appeared earlier than in the nontreated group, and localization at the site of trauma was more frequent (4/9) than in the nontreated hamsters (1/5). nine of the 11 strains (82%) were capable of repr ... | 1988 | 3193329 |
| lr1: a candidate rna virus of leishmania. | although viruses are important biological agents and useful molecular tools, little is known about the viruses of parasites. we report here the discovery of a candidate for an rna virus in a kinetoplastid parasite. this potential virus, which we term lr1, is present in the promastigote form of the human pathogen leishmania braziliensis guyanensis cumc1-1a but not in 11 other stocks of leishmania that were examined nor in trypanosoma brucei. the candidate viral rna has a size of approximately 600 ... | 1988 | 3200841 |
| in vitro interaction of acantamoeba castellani with leishmania brasiliensis. | 1988 | 3208254 | |
| epidemiology of a natural focus of leishmania braziliensis in the pacific lowlands of colombia. iii. natural infections in wild mammals. | a natural infection of leishmania braziliensis panamensis in the edentate, choloepus hoffmanni is recorded from the pacific coast of columbia. | 1988 | 3232170 |
| epidemiology of a natural focus of leishmania braziliensis in the pacific lowlands of colombia. iv. observations on sandflies. | 1988 | 3232171 | |
| predominance of leishmania braziliensis braziliensis in the regions of três braços and corte de pedra, bahia, brazil. | 1988 | 3232172 | |
| cutaneous leishmaniasis from guatemala or yucatan due to leishmania braziliensis braziliensis. | 1988 | 3235257 | |
| low dose glucantime therapy in leishmania viannia braziliensis (lvb) infections. | 1988 | 3249826 | |
| [active antihelminitic alkaloids: active in vitro against leishmanic tropica the protozoa involved in leishmaniasis]. | leishmaniasis caused by protozoan leishmania ssp., is an endemic parasitic disease in central and south america. the chemotherapeutic agents against leishmania ssp. (pentavalent antimony compounds, pentamidine and amphothericine b) are toxic and expensive products. basing on the bolivian folk medicine, we tried to find new active principles. fourteen isoquinoline alkaloids, especially bisbenzylisoquinoline alkaloids extracted from annonaceae, berberidaceae, hernandiaceae and menispermaceae, demo ... | 1988 | 3253497 |
| identification and purification of a 72 kda antigen of leishmania braziliensis braziliensis present on the surface and in the cytoplasm of the promastigotes and its specific recognition by sera from mucocutaneous leishmaniasis patients. | 1988 | 3253506 | |
| epidemiological studies on american leishmaniasis in ceará state, brazil. molecular characterization of the leishmania isolates. | two methods of molecular characterization, using monoclonal antibodies and enzyme electrophoresis, were employed in the identification of 36 stocks of leishmania isolated from human and canine cases of american visceral (avl) and cutaneous (acl) leishmaniases in the northern part of ceará state, brazil. molecular homogeneous strains of leishmania donovani (chagasi) isolated from both human and canine hosts were detected in 14 municipalities. two more parasite species, l. braziliensis braziliensi ... | 1988 | 3256276 |
| quantification of leishmania-specific t cells in human american cutaneous leishmaniasis (leishmania braziliensis braziliensis) by limiting dilution analysis. | limiting dilution analysis was used to estimate the frequency of leishmania-specific t cells from the peripheral blood of 18 human cases of american cutaneous leishmaniasis (acl). sixteen patients had localized cutaneous leishmaniasis (lcl) and two were recovered lcl patients. in 10 patients with active disease and in two with healed lesions the leishmania-specific t cell frequencies ranged from 1/10(5) to 1/10(3). in six patients no proliferation was detected after 21 days of cell culture. this ... | 1988 | 3258203 |
| natural infection of lutzomyia ovallesi (diptera: psychodidae) with parasites of the leishmania braziliensis complex in a restricted focus of cutaneous leishmaniasis in northern venezuela. | 1988 | 3271937 | |
| an outbreak of american cutaneous leishmaniasis (leishmania braziliensis braziliensis) in a periurban area of rio de janeiro city, brazil: clinical and epidemiological studies. | from july 1984 to september 1986, 105 cases of american cutaneous leishmaniasis were studied in a locality closely situated to an urbanized area of the city of rio de janeiro, brazil. settlement in this area was established at least 20 years ago but the first cases were noted six months prior to the beginning of this study. cases were almost exclusively cutaneous and ulcerated, with one to six months of evolution. montenegro's skin tests were positive in all cases and anti-leishmania antibodies ... | 1988 | 3271941 |
| isolation of leishmania guyanensis from lesions of the nasal mucosa. | 1988 | 3271946 | |
| canine american cutaneous leishmaniasis: a clinical and immunological study in dogs naturally infected with leishmania braziliensis braziliensis in an endemic area of rio de janeiro, brazil. | clinical and immunological findings from 35 dogs infected with leishmania braziliensis braziliensis are described. the majority of the dogs had ulcerated single lesions on the ears. sera from all infected dogs showed detectable leishmania-induced antibodies using an indirect fluorescent antibody test. antimonial therapy resulted in prompt healing of the lesions in 80.9% of the animals followed by a significant reduction in the anti-leishmania antibody titers. however, treatment follow-up showed ... | 1988 | 3277465 |
| experimental canine mucocutaneous leishmaniasis (leishmania braziliensis braziliensis). | four mongrel dogs were intradermically inoculated with 3 x 10(6) leishmania braziliensis braziliensis promastigotes. three out of the four animals developed cutaneous lesions respectively 4, 7, and 8 months after. the fourth dog did not develop lesion at the inoculation site, but a mucosal ulcer was seen 16 months after the inoculum. clinical, histopathological, and serological findings were similar to what is found in natural canine infection as well as in the human disease. these results sugge ... | 1988 | 2687621 |
| retention of antimony in hair during leishmaniasis treatment. | 1989 | 2714007 | |
| morphology of leishmania braziliensis: changes during reversible heat-induced transformation from promastigote to an ellipsoidal form. | leishmania braziliensis, growing axenically at 26 c and transferred to 34 c, changes within 3 hr from the long slender motile promastigote form to an ellipsoidal form with a nonmotile flagellum. this transformation is reversible for heat treatments of up to 12 hr. in this study we show by light microscopic measurements that the cells decrease in length and increase in diameter at constant volume. quantitative morphometry of electron micrographs further demonstrates that: the distance between nuc ... | 1989 | 2723927 |
| metabolic interactions between glucose, glycerol, alanine and acetate in leishmania braziliensis panamensis promastigotes. | 13c-nuclear magnetic resonance (nmr) spectroscopy was used to investigate the products of glycerol and acetate metabolism released by leishmania braziliensis panamensis promastigotes and also to examine the interaction of each of these substrates with glucose or alanine. the nmr data were supplemented by measurements of the rates of oxygen consumption and substrate utilization, and of 14co2 production from 14c-labeled substrate. cells incubated with [2-13c]glycerol released acetate, succinate an ... | 1989 | 2724185 |