Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| outbreak of bronchiolitis obliterans associated with consumption of sauropus androgynus in taiwan. | in august, 1995, there was an outbreak in taiwan of rapidly progressive respiratory distress associated with consumption of uncooked sauropus androgynus, a vegetable with a claimed yet unconfirmed effect on weight control. we report on 23 patients with strikingly similar clinical presentations. | 1996 | 8676721 |
| removal of necrotic tissue with an ananain-based enzyme-debriding preparation. | an enzymatic debriding preparation was formulated with purified enzyme derived from a crude pineapple stem extract. the primary component of this preparation was the sulfhydryl protease ananain which represented >/=85% of the proteolytic activity. the remaining proteolytic activity in the preparation was contributed by a co-purifying homologous cysteine protease comosain. taken together these two proteases provided a protein purity of greater than 95% as judged by sodium dodecyl sulfate polyacry ... | 1996 | 17309694 |
| changes in plant-parasitic nematode populations in pineapple fields following inter-cycle cover crops. | the use of plant-covers oat (arena sativa l.), rhodesgrass (chloris gayana kunth), soybean (glycine max [l.] merr.), and marigold (tagetes patula l.) during pineapple inter-cycle planting periods was investigated at two sites (kunia and whitmore, oahu, hi) as a potential means to reduce population densities of rotylenchulus reniformis, helicotylenchus dihystera, and paratylenchus spp. clean fallow and fallow covered with pineapple-plant residues (mulch) were the controls without plant-cover. reg ... | 1996 | 19277173 |
| bromelain prevents secretion caused by vibrio cholerae and escherichia coli enterotoxins in rabbit ileum in vitro. | diarrhea is a major cause of illness and death in children and young animals. the aim of this study was to investigate the possible therapeutic effect of bromelain, a proteolytic extract obtained from pineapple stems on bacterial toxin and second-messenger agonist-induced intestinal secretion. | 1997 | 9207276 |
| shelf life study of some nigerian fruit juices inoculated with ascospores of neosartorya spp. | mango, orange and pineapple juices containing various concentrations of sucrose were dispensed in 100 ml volumes into 100 ml conical flasks with stoppers. each juice in a flask was inoculated with ascospores of one local isolate of either neosartorya fischeri, n. fischeri var. spinosa or n. quadricincta to a final concentration of 120-140 ascospores/ml of juice, and then pasteurized at 80 degrees c for 30 min. a set of 36 inoculated flasks with 12 controls was stored at 4-5 degrees c and two set ... | 1997 | 9477427 |
| evaluation of growth of neosartorya spp. on some nigerian fruit products. | growth of neosartorya fischeri, n. fischeri var. spinosa and n. quadricincta on inoculated fruit juice agars (fjas) consisting of 1% agar and juices from mango (mja), orange (oja) and pineapple (pja) containing various levels of added sucrose was studied in terms of the average number of colonies produced by their ascospores, time (days) required before colonies were formed and developed to confluent mycelial mat as well as the level of reproductive spores produced. the means of the number of co ... | 1997 | 9650724 |
| inhibition of the mutagenicity of 2-nitrofluorene, 3-nitrofluoranthene and 1-nitropyrene by vitamins, porphyrins and related compounds, and vegetable and fruit juices and solvent extracts. | when 21 vitamins including related compounds haemin, chlorophyllin, chlorophyll, biliverdin and bilirubin, as well as juices from five fruits and 25 vegetables and solvent extracts from the residues of fruits and vegetables were tested for their antimutagenic potencies with respect to mutagenicity induced by 2-nitrofluorene (2-nf), 3-nitrofluoranthene (3-nfa) and 1-nitropyrene(1-np) in salmonella typhimurium ta98 the following results were obtained. the tetracyclic nitroarenes 3-nfa and 1-np wer ... | 1997 | 9207899 |
| immunological cross-reactivity of the major allergen from perennial ryegrass (lolium perenne), lol p i, and the cysteine proteinase, bromelain. | antibodies prepared in rabbits against the major allergen from ryegrass (lolium perenne), lol p i, cross-reacted with the cysteine proteinase bromelain from pineapple and vice versa. deglycosylation of the proteins showed that the cross-reaction was based on recognition of the carbohydrate moiety of the allergen, but for bromelain the cross-reaction was most likely due to a combination of factors. the results indicate that the carbohydrate residues from these allergens play an important role in ... | 1997 | 9104799 |
| complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. | the amino acid sequences of ananain (ec3.4.22.31) and stem bromelain (3.4.22.32), two cysteine proteases from pineapple stem, are similar yet ananain and stem bromelain possess distinct specificities towards synthetic peptide substrates and different reactivities towards the cysteine protease inhibitors e-64 and chicken egg white cystatin. we present here the complete amino acid sequence of ananain and compare it with the reported sequences of pineapple stem bromelain, papain and chymopapain fro ... | 1997 | 9355753 |
| recent case histories of food product-metal container interactions using scanning electron microscopy-x-ray microanalysis. | scanning electron microscopy (sem) and x-ray microanalysis (eds) were used to investigate stress corrosion cracking (scc) in plain tinplate food cans, product discoloration, filiform corrosion, pitting/perforation corrosion, and loss of coating adhesion in enameled food cans. intergranular scc of uncoated tinplate occurred in canned pineapple juice. this is a rare occurrence in acid food products. the fracture developed in the can headspace only at the product line/metal interface by an interact ... | 1997 | 9353869 |
| intestinal absorption of undegraded proteins in men: presence of bromelain in plasma after oral intake. | the human adult intestinal epithelium has traditionally been described as nonpermeable to proteins. however, indirect evidence suggests that reduced absorption of undegraded proteins might take place under physiological conditions. using bromelain (an enzyme obtained from pineapple stems) as a model protein, we studied the extent of this mucosal permeation in 19 healthy men. the protein was detected in plasma by immunoassay and by its proteolytic activity after oral administration. the estimated ... | 1997 | 9252520 |
| identification and characterization of a pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple. | pantoea citrea, a member of the family enterobacteriaceae, causes pink disease of pineapple, whose symptom is characterized by the formation of pink to brown discolorations of the infected portions of the pineapple fruit cylinder upon canning. molecular genetic approaches were applied to elucidate the mechanism responsible for this fruit discoloration. a p. citrea mutant strain, cmc6, defective in its ability to cause pink disease and fruit discoloration, was generated by nitrosoguanidine mutage ... | 1997 | 8979341 |
| a shipyard outbreak of salmonellosis traced to contaminated fruits and vegetables. | a large outbreak of food poisoning occurred in september 1996 and involved at least 116 workers at a shipyard in jurong. salmonella weltevreden was isolated from the stool specimens of 24 hospitalised cases and three food handlers, giving a total of 27 bacteriologically confirmed cases (25 symptomatic and two asymptomatic) in this outbreak. based on a case-control analysis, a number of food items consumed on 23 and 24 september 1996 were implicated in the outbreak, viz. a vegetable dish (p < 0.0 ... | 1997 | 9494655 |
| the prevalence of latex allergy in children seen in a university hospital allergy clinic. | natural rubber latex allergy is responsible for a wide spectrum of clinical symptoms, ranging from rhinoconjunctivitis to severe anaphylaxis, in both adults and children. an association between allergy to latex and allergy to various fruits has been reported. this study investigated the prevalence and clinical significance of latex sensitization in children seen in a university hospital allergy clinic. a total of 453 consecutive children were screened in a 7-month period. a detailed clinical his ... | 1997 | 9062637 |
| [physico-chemical characterization of acerola (malpighia glabra l.) produced in maringá, paraná state, brazil]. | the acerola malpighia glabra l., originally from the antillas and north of south america, known by the people as cereja-das-antilhas or cereja-do-pará distinguish itself by its high content of vitamin c. the ripe and fresh acerola fruits utilized in experiments, were obtained from farmers of maringá region, paraná state, brazil. the fruits were hulled in steel sieve with 25 mesh and the bagasse (seeds and hull) discarded. these physico-chemical analysis were realized in the pulp: vitamin c, mois ... | 1997 | 9429646 |
| [cross-reactivity between bromelain and soluble fraction from wheat flour]. | it is revealed that patients allergic to a water-soluble fraction from wheat flour were sensitive to pineapple enzyme, bromelain. since bromelain has a high similarity to other sh-proteases such as papain, it may imply that the patients may recognize varieties of other sh-proteases as the epitope. | 1997 | 9436335 |
| pollination increases gibberellin levels in developing ovaries of seeded varieties of citrus. | reproductive and vegetative tissues of the seeded pineapple cultivars of sweet orange (citrus sinensis l.) contained the following c-13 hydroxylated gibberellins (gas): ga53, ga17, ga19, ga20, ga1, ga29, and ga8, as well as ga97, 3-epi-ga1, and several uncharacterized gas. the inclusion of 3-epi-ga1 as an endogenous substance was based on measurements of the isomerization rates of previously added [2h2]ga1. pollination enhanced amounts of ga19, ga20, ga29, and ga8 in developing ovaries. levels o ... | 1997 | 12223728 |
| "latex-fruit syndrome": frequency of cross-reacting ige antibodies. | an association between allergies to latex proteins and to various foods has been reported and confirmed by rast and immunoblotting inhibition. however, no significant data had been collected on the frequency of specific ige antibodies to fruits in these patients and the frequency of a history of fruit intolerance. serum samples of 136 patients with well-documented, clinically relevant, immediate-type hypersensitivity against latex proteins were analyzed for ige antibodies against a panel of diff ... | 1997 | 9188921 |
| purification and structural and kinetic characterization of the pyrophosphate:fructose-6-phosphate 1-phosphotransferase from the crassulacean acid metabolism plant, pineapple. | pyrphosphate-dependent phosphofructokinase (pfp) was purified to electrophoretic homogeneity from illuminated pineapple (ananas comosus) leaves. the purified enzyme consists of a single subunit of 61.5 kd that is immunologically related to the potato tuber pfp [beta] subunit. the native form of pfp likely consists of a homodimer of 97.2 kd, as determined by gel filtration. pfp's glycolytic activity was strongly dependent on ph, displaying a maximum at ph 7.7 to 7.9. gluconeogenic activity was re ... | 1997 | 12223643 |
| prevalence of and risk factors for latex sensitization in patients with spina bifida. | we determined the prevalence of and risk factors for latex sensitization in patients with spina bifida. | 1998 | 9783950 |
| retrotransposon-like sequences integrated into the genome of pineapple, ananas comosus. | retrotransposon-like sequences have been serendipitously detected in the genome of commercial pineapple, ananas comosus. the sequence from a 2.6 kb cloned fragment of this element had greatest similarity to the del1 lilium henryi retrotransposon and the gypsy/ty3 group of retroelements. the order of the genes from 5' to 3' was reverse transcriptase, ribonuclease h and integrase. the integrase domain contained the amino acid sequence motifs which have been associated with recognition of the long ... | 1998 | 9747853 |
| determination of soil-to-plant transfer factors of 137cs and 90sr in the tropical environment of bangladesh. | soil-to-plant transfer factors (tf) of 137cs and 90sr have been determined for different plants/crops, such as rice, beans, peanuts, pineapple, cabbage, tomato, spinach and grass. they were obtained from radioisotope experiments on plants grown in pots under outdoor ambient tropical conditions for three growing seasons (1994-1996). in the case of 137cs and concerning the above mentioned plants/crops, the average tfs were found to be 0.28, 0.25, 0.77, 0.19, 0.23, 0.28, 0.59 and 0.18, respectively ... | 1998 | 9728745 |
| the amino acid sequences of isoforms of the bromelain inhibitor from pineapple stem. | eight bromelain inhibitor (bi) isoform fractions were isolated from pineapple stem, and then submitted to conventional amino acid sequencing after performic acid oxidation and subsequent separation of the resulting light and heavy chains. the results revealed that all fractions exhibited microheterogeneity, containing at least two major components, but that all the isoinhibitors have a common double-chain structure (mr = ca. 5,700-5,900) with five disulfide bonds and similar amino acid sequences ... | 1998 | 9685742 |
| method for the quantitative determination of 1-naphthaleneacetic acid in spiked canned pineapple samples by micelle-stabilized room temperature phosphorescence. | this paper presents a convenient method for the determination of 1-naphthaleneacetic acid (naa) plant growth regulator in spiked canned pineapple samples by micellar-stabilized room temperature phosphorescence with sodium dodecyl sulfate as a micellar medium, thallium nitrate as an external heavy atom, and sodium sulfite as an oxygen scavenger. a multivariate optimization approach using the type of central composite blocked cube-star design was carried out. the analytical curve of naa gives a li ... | 1998 | 10554277 |
| isolation and characterization of two forms of an acidic bromelain stem proteinase. | two forms of an acidic bromelain proteinase isolated from crude bromelain, an extract from pineapple stem, were found by a two-step fplc purification procedure. the basic main components were removed by cation exchange chromatography and the breakthrough fraction was further resolved by anion exchange chromatography into 15 protein fractions, only two of which, called sba/a and sba/b, were proteolytically active. these components were characterized by electrospray mass spectroscopy (esms), isoel ... | 1998 | 9619588 |
| influence of growth medium on thermal resistance of pediococcus sp. nrrl b-2354 (formerly micrococcus freudenreichii) in liquid foods. | pediococcus sp. is a nonpathogenic heat-resistant spoilage organism that has been used as a test organism in milk pasteurization studies. these characteristics make this bacterium an attractive test organism to study the mode of bacterial thermal inactivation in a food pilot plant. we report here the effect of growth medium on the thermal d value of this organism in skim milk, whole liquid egg, 10% glucose solution, pineapple juice, apple juice, tomato juice, and water at 60 degrees c. thermal i ... | 1998 | 9709230 |
| polymerase chain reaction for rapid detection of campylobacter jejuni in artificially contaminated foods. | campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. these food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (pcr). specific detection of camp. jejuni, as indicated by a pcr product of 159 bp, was possible with all pre-cooked deli-sliced and raw poultry products tested. all vegetables tested were compatible with the pcr assay. cantaloupe, kiwi and pineapple tested positive while strawberries, watermelon, grapes and ... | 1998 | 9750320 |
| bromelain protects piglets from diarrhoea caused by oral challenge with k88 positive enterotoxigenic escherichia coli. | k88 positive enterotoxigenic escherichia coli (k88+ etec) is an important cause of diarrhoea in young piglets. k88+ etec pathogenesis relies on attachment to specific glycoprotein receptors located on the intestinal mucosa. proteolytic treatment of these receptors in vitro and in vivo prevents attachment of k88+ etec to piglet small intestines and may be of clinical use to prevent k88+ etec pathogenesis. | 1998 | 10189844 |
| inactivation of microorganisms with microwaves at reduced temperatures. | we developed a pilot-plant nonthermal flow process using microwave energy to inactivate microorganisms. the process consists of multiple passes through the microwave generator. each passed material goes to a receiving tank for subsequent passes. the flow rate was 0.96 to 1.26 kg/min and the dwell time per pass was 1.1 to 1.5 min. five passes were used. the microwave energy is instantaneously and simultaneously applied to the system, and thermal energy is removed by a cooling tube within the proc ... | 1998 | 9709231 |
| characterization of pucd5000 involved in pink disease color formation by pantoea citrea. | pantoea citrea, the causal agent of pink disease of pineapple, harbors a cryptic plasmid of 5229 bp. designated pucd5000. on the basis of nucleotide and amino acid sequence analyses, pucd5000 contains both replication and mobilization loci (bom and mobcabd) that are similar to those in plasmids psw100 and psw200 in pantoea stewartii and pec3 in erwinia carotovora subsp. carotovora. the survival of p. citrea on pineapple does not depend on pucd5000. however, full pink coloration development, whic ... | 1998 | 9735319 |
| [protein hydrolysate as a dietetic resource] | objective: the central goal of this paper was to study the application of beef meat and poultry (turkey and chicken) hydrolysates to the preparations used in our brazilian current feeding practices. methods: the various kinds of meat were hydrolyzed with fresh pineapple under similar conditions to those daily used at home. the selection of three types of preparation was dependent on whether their contents included starch or gelatin and liquid, like soup, mousse and fruit-shake. hydrolysate were ... | 1998 | 14685623 |
| transmission of pineapple mealybug wilt-associated virus by two species of mealybug (dysmicoccus spp.). | abstract closterovirus-like particles associated with mealybug wilt of pineapple were acquired and transmitted by the pink pineapple mealybug, dysmicoccus brevipes, and the gray pineapple mealybug, d. neobrevipes. mealybugs acquired pineapple mealybug wilt-associated virus (pmwav) from infected pineapple plants or detached leaves. the virus was detected in plants by tissue blot immunoassay and confirmed by immunosorbent electron microscopy. plants exposed to mealybugs reared on pmwav-free pineap ... | 1998 | 18944858 |
| investigation of the utility of pineapple juice and pineapple waste material as low-cost substrate for ethanol fermentation by zymomonas mobilis. | the utility of the juice of rotten or discarded pineapples and the waste material of the production of pineapple juice as low-cost substrates for ethanol production by zymomonas mobilis was investigated. z. mobilis atcc 10988 produced 59.0 g.l(-1) ethanol in undiluted pineapple juice without nutritional supplementation and without the regulation of the ph while 42.5 g.l(-1) ethanol was obtained using a 125 g.l(-1) sucrose medium supplemented with 10 g.l(-1) yeast extract and mineral salts. ethan ... | 1999 | 16232532 |
| the naturally occurring furanones: formation and function from pheromone to food. | three closely related 4-hydroxy-3(2h)-furanones have been found in a range of highly cooked foodstuffs where they are important flavour compounds with aroma threshold values as low as 20 micrograms kg-1 water (approximately 0.14 mumol l-1). the compounds are formed mainly as a result of the operation of the maillard reactions between sugars and amino acids during heating but one compound, 5-(or 2)-ethyl-2-(or 5)-methyl-4-hydroxy-3(2h)-furanone, appears in practice to be produced by yeast, probab ... | 1999 | 10466251 |
| antimutagenic effect of fruit and vegetable ethanolic extracts against n-nitrosamines evaluated by the ames test. | the inhibitory effect of nine fruit and vegetable ethanolic extracts against the mutagenicity of n-nitrosodimethylamine (ndma), n-nitrosopyrrolidine (npyr), n-nitrosodibutylamine (ndba), and n-nitrosopiperidine (npip) was evaluated by means of the ames test. licorice ethanolic extract was the only one that showed an inhibitory effect (ranging from moderate to strong) against mutagenicity of all n-nitrosamines tested. this ethanolic extract showed the greatest inhibition effect against npip (72%) ... | 1999 | 10552641 |
| intestinal absorption of calcium from foodstuffs as compared to a pharmaceutical preparation. | only few data are available on intestinal calcium absorption from foodstuffs and composite meals in humans. the aim of the study was to compare intraindividually the calcium absorption from milk and from a breakfast with that from a pharmaceutical calcium preparation of equal calcium content. in 8 healthy volunteers between 44 and 58 years of age, the intestinal calcium absorption was measured in randomized order applying the double isotope technique from: (1) 500ml of fresh milk (equivalent to ... | 1999 | 10902536 |
| comparative content of total polyphenols and dietary fiber in tropical fruits and persimmon. | recent studies have shown that dietary fiber and polyphenols of vegetables and fruits improve lipid metabolism and prevent the oxidation of low density lipoprotein cholesterol (ldl-c), which hinder the development of atherosclerosis. the goal of this study was to measure the total polyphenol and dietary fiber contents of some tropical fruits (i.e., pineapple, wax apple, rambutan, lichi, guava, and mango) and compare the results to the content of these substances in the better characterized persi ... | 1999 | 15539312 |
| [assessment of the impact of the biological rodenticide biorat in populations of rodents settled in several crops of the republic of costa rica]. | the impact of biological rodenticide biorat on populations of rodents settled in some crops was determined; these crops are highly infested with sigmodon hispidus, a very common vector in various central american countries. the trapping technique and the appraisal of damages allowed to calculate the index of infestation which ranged from 75 to 90 percent in three crops. twenty for to 36 kg of biorat were applied to 6 ha planted with cucumis sativus (cucumber), ipomoea batata (sweet potato) and a ... | 1999 | 10887586 |
| involvement of carbohydrate epitopes in the ige response of celery-allergic patients. | this study was performed to get further insights into antibody responses to cross-reactive carbohydrate determinants (ccd), including initial experiments to prove the biological activity of anti-ccd ige. earlier studies have shown that ige specific for ccd occurs in about 25% of celery-allergic patients. the clinical significance of these antibody specificities is doubtful. | 1999 | 10529586 |
| differentiation of fusarium subglutinans f. sp. pini by histone gene sequence data. | fusarium subglutinans f. sp. pini (= f. circinatum) is a pathogen of pine and is one of eight mating populations (i.e., biological species) in the gibberella fujikuroi species complex. this species complex includes f. thapsinum, f. moniliforme (= f. verticillioides), f. nygamai, and f. proliferatum, as well as f. subglutinans associated with sugarcane, maize, mango, and pineapple. differentiating these forms of f. subglutinans usually requires pathogenicity tests, which are often time-consuming ... | 1999 | 10427026 |
| effect on cell surface hydrophobicity and susceptibility of helicobacter pylori to medicinal plant extracts. | effects on aqueous extracts of medicinal plants on ten helicobacter pylori strains were studied by the salt aggregation test to determine the possibility to modulate their cell surface hydrophobicity and by an agar diffusion assay for detection of antimicrobial activity. it was established that aqueous extracts of bearberry and cowberry leaves enhance cell aggregation of all h. pylori strains tested by the salt aggregation test, and the extract of bearberry possessed a remarkable bacteriostatic ... | 1999 | 10079525 |
| bromelain proteases reduce human platelet aggregation in vitro, adhesion to bovine endothelial cells and thrombus formation in rat vessels in vivo. | the thiol protease, bromelain, an extract from pineapple stem, was suggested to have antithrombotic and anticoagulant activities in vivo. we studied the effects of bromelain on cell size distribution of isolated human platelets in vitro by coulter counter measurements. preincubation of platelets with bromelain (10 micrograms/ml) completely prevented the thrombin (0.2 u/ml) induced platelet aggregation. papain was less active in preventing platelet aggregation. in vitro, bromelain (0.1 microgram/ ... | 1999 | 10218125 |
| gdhb, a gene encoding a second quinoprotein glucose dehydrogenase in pantoea citrea, is required for pink disease of pineapple. | the pink disease of pineapple, caused by the bacterium pantoea citrea, is characterized by a dark coloration on fruit slices after canning. a glucose dehydrogenase (gdh) encoded by the gdha gene has been implicated in the colour formation activity of p. citrea. in this paper it has been shown that p. citrea contains a second, homologous gdh gene and its product, gdhb, represents the main source of gdh activity in this organism. unlike gdha, gdhb is constitutively expressed during the exponential ... | 1999 | 10376838 |
| investigation of an outbreak of gastroenteritis at a hospital for patients with learning difficulties. | eighty of the 460 patients and staff (attack rate 22%) in a long-stay hospital for patients with learning difficulties became ill in a general outbreak of gastrointestinal infection that followed a buffet style party on one ward. illness in the cohort of 47 resident patients, relations, and staff who attended that party was associated with having eaten ham, coleslaw, bread rolls, and cheese and pineapple on sticks. food from the hospital kitchen was supplemented by food brought in and prepared o ... | 1999 | 10462893 |
| specific natural isotope profile studied by isotope ratio mass spectrometry (snip-irms): (13)c/(12)c ratios of fructose, glucose, and sucrose for improved detection of sugar addition to pineapple juices and concentrates. | the delta(13)c values of fructose, glucose, and sucrose have been determined in authentic pineapple juices. the sugar fraction is separated from the organic acids by an anionic exchange process. then the individual components (fructose, glucose, and sucrose) are isolated on a preparative hplc device using a nh(2)-type column. it is demonstrated that no significant isotope fractionation occurs when close to 100% of material is recovered and when the hydrolysis of sucrose is avoided. the control o ... | 1999 | 10794628 |
| risk factors for latex allergy in patients with spina bifida and latex sensitization. | some subjects with spina bifida (sb) and latex sensitization (cutaneous and/or serum ige) can have clinical reactions, while others may have no symptoms after the exposure to latex products. | 1999 | 10231329 |
| [spherical phenyl-regenerated cellulose stationary phases used for hydrophobic chromatography to extract bramelain from crude stem bramelain]. | the low-pressure preparative hydrophobic chromatography packing materials have been successfully synthesized by grafting hydrophobic phenyl group to the macro-pore spherical regenerated cellulose resin (diameter 0.7-1.3 mm, pore size 4.3-15.7 microns, specific surface area 88 m2/g). the optimum adsorption-desorption conditions have been got by using 10 ml of this hydrophobic resin to deal with 100 ml of the crude stem bramelain: the mobile phase ph is around 5.0, concentration of (nh4)2so4 is 1. ... | 1999 | 12549169 |
| structural characterization of the n-linked oligosaccharides from tomato fruit. | the primary structures of the n-linked oligosaccharides from tomato fruit (lycopersicon esculentum) have been elucidated. for the isolation of the protein fraction, two procedures were employed alternatively: a low temperature acetone powder method and ammonium sulfate precipitation of the tomato extract. after peptic digestion, the glycopeptides were purified by cation-exchange chromatography; the oligosaccharides were released by n-glycosidase a and fluorescently labelled with 2-aminopyridine. ... | 1999 | 10365448 |
| incidence of patulin in fruits and fruit juices marketed in campinas, brazil. | one hundred and eleven samples of processed fruit juices (apple, grape, pineapple, papaya, guava, banana and mango) and 38 samples of sound fruits (apple, papaya, mango, pear and peach) produced and marketed in brazil, were analysed for patulin by hplc. only one out of 30 samples of apple juice was found positive at 17 micrograms/l. patulin was not detected in the other foodstuffs. it was found in 14 samples of spoiled fruit samples of apple (150-267 micrograms/kg), pear (134-245 micrograms/kg) ... | 1999 | 10435075 |
| role of carbohydrate moieties in ige binding to allergenic components of cupressus arizonica pollen extract. | a reduction of ige immunoreactivity after periodate-treatment has been previously reported for various glycoprotein allergens. | 1999 | 10457113 |
| bromelain, from pineapple stems, proteolytically blocks activation of extracellular regulated kinase-2 in t cells. | recently, it has emerged that extracellular proteases have specific regulatory roles in modulating immune responses. proteases may act as signaling molecules to activate the raf-1/extracellular regulated kinase (erk)-2 pathway to participate in mitogenesis, apoptosis, and cytokine production. most reports on the role of protease-mediated cell signaling, however, focus on their stimulatory effects. in this study, we show for the first time that extracellular proteases may also block signal transd ... | 1999 | 10452995 |
| routes of ethephon uptake in pineapple (ananas comosus) and reasons for failure of flower induction. | ethylene-releasing agents such as ethephon (2-chloroethylphosphonic acid) are used widely to induce flowering in pineapples (ananas comosus (l.) merrill). however, ethephon treatment is less reliable in summer, particularly if plants are treated on abnormally hot days. [(14)c]ethephon was used to follow uptake and translocation in leaf tissues. up to 30% of the ethephon entered the leaf within 4 h, and up to 60% by 24 h. uptake was dramatically modified by temperature, relative humidity, solutio ... | 1999 | 10688702 |
| combinatorial approach to flavor analysis. 2. olfactory investigation of a library of s-methyl thioesters and sensory evaluation of selected components. | the odor characteristics of individual components present in a library comprised of s-methyl thioesters were determined independently by two laboratories using similar but not identical techniques. the odor potency was assessed by values of best estimate-gc-lower amount detected by sniffing (be-gc-loads). for small and medium chain s-methyl thioesters, these values were found to increase from 6 ng for s-methyl thiobutanoate to 90 ng for s-methyl thiostearate. all assessors detected a "green", "f ... | 1999 | 10552644 |
| characterization of sinapyl derivatives in pineapple (ananas comosus [l.] merill) juice. | three previously unidentified phenolic compounds were found in pineapple (ananas comosus [l.] merill) juice in substantial concentrations and were isolated by semipreparative reverse phase hplc. the structures were elucidated from uv spectra, acid hydrolysis, and subsequent amino acid analysis, mass spectrometry, and two-dimensional nmr spectroscopy. the compounds are identified as s-sinapyl-l-cysteine, n-l-gamma-glutamyl-s-sinapyl-l-cysteine, and s-sinapylglutathione. | 1999 | 10552379 |
| purification and characterization of macrodontain i, a cysteine peptidase from unripe fruits of pseudananas macrodontes (morr.) harms (bromeliaceae). | a new papain-like cysteine peptidase isolated from fruits of pseudananas macrodontes (morr.) harms, a species closely related to pineapple (ananas comosus l.), has been purified and characterized. the enzyme, named macrodontain i, is the main proteolytic component present in fruit extracts and was purified by acetone fractionation followed by anion-exchange chromatography. separation was improved by selecting both an adequate ph value and a narrow saline gradient. optimum ph range (more than 90% ... | 2000 | 10686143 |
| food entrapped in papilla of vater: uncommon cause of vomiting. | a 20 month old girl was admitted for intractable vomiting over several days, with no other symptoms. family and personal history were not contributive. clinical and neurological examination, and routine blood tests and investigations (plain abdominal x ray, upper gastrointestinal tract contrast study, abdominal ultrasonography) were normal. the upper gastrointestinal endoscopy showed a mild antral gastritis and the second portion of duodenum was occupied by a tough, fibrous mass partially embedd ... | 2000 | 10833184 |
| blood glucose responses of diabetes mellitus type ii patients to some local fruits. | to determine the glucose responses of diabetes mellitus type ii subjects to fruits, four locally available fruits (containing 25 g of available carbohydrates per serving portion) of chico, mango, pineapple, and papaya were tested among ten type ii diabetic subjects, using wheat bread as the control. results of the in vivo test indicated that chico and mango had significantly lower (p ≤ 0.05) blood glucose areas compared to wheat bread. chico and mango also had a much lower glycemic index (gi), 5 ... | 2000 | 24394507 |
| high-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits. | a high-performance liquid chromatographic (hplc) procedure based on an isocratic elution with photodiode array detection has been developed for a simple and rapid determination of ellagic acid (ea) in fresh and processed fruits. the homogenized sample was refluxed with methanol and then the extract was refined using a solid-phase cartridge before hplc. we analyzed ea in 40 kinds of fresh fruits and 11 kinds of processed fruits by the developed method. ea was found in several berries, fueijoa, pi ... | 2000 | 11093643 |
| enzymatic synthesis of stable, odorless, and powdered furanone glucosides by sucrose phosphorylase. | sucrose phosphorylase from leuconostoc mesenteroides catalyzed transglucosylation from sucrose to 4-hydroxy-3(2h)-furanone derivatives. when 4-hydroxy-2,5-dimethyl-3(2h)-furanone (hdmf) and 2-ethyl-4-hydroxy-5-methyl-3(2h)-furanone or 5-ethyl-4-hydroxy-2-methyl-3(2h)-furanone (ehmf) were used as acceptors, their transfer ratios were more than 45%. in the case of glucosylation of hdmf, the major transfer product was identified as 2,5-dimethyl-3(2h)-furanone 4-o-alpha-d-glucopyranoside (dmf-g). in ... | 2000 | 10705458 |
| genetic and biochemical characterization of the pathway in pantoea citrea leading to pink disease of pineapple. | pink disease of pineapple, caused by pantoea citrea, is characterized by a dark coloration on fruit slices after autoclaving. this coloration is initiated by the oxidation of glucose to gluconate, which is followed by further oxidation of gluconate to as yet unknown chromogenic compounds. to elucidate the biochemical pathway leading to pink disease, we generated six coloration-defective mutants of p. citrea that were still able to oxidize glucose into gluconate. three mutants were found to be af ... | 2000 | 10735866 |
| natural endophytic occurrence of acetobacter diazotrophicus in pineapple plants. | the presence of endophytic acetobacter diazotrophicus was tested for pineapple plants (ananas comosus [l.] merr.) grown in the field. diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. phenotypic tests permitted the selection of presumptive nitrogen-fixing a. diazotrophicus isolates. restriction fragment length polymorphisms (rflps) of small subunit (ssu) rdna using total dna digested with endonuclease sphi and with endo ... | 2000 | 10790517 |
| occurrence and significance of cryptococcus neoformans in vegetables and fruits. | cryptococcus neoformans was isolated from three of 437 (0.6%) samples of a wide variety of vegetables collected from a number of markets in delhi. the vegetables yielding c. neoformans were tomato, lycopersicon esculentum (1/70), 'vegetable sponge', luffa cylindrica (1/56) and brinjal, solanum melongena (1/55). only solitary samples of these three vegetables were found to be positive. in vitro studies at 25 degrees c revealed that a reference c. neoformans isolate readily colonized, as did candi ... | 2000 | 15597680 |
| mutagenesis and kinetic studies of a plant cysteine proteinase with an unusual arrangement of acidic amino acids in and around the active site. | we report the cloning, overexpression, kinetic analysis, and modeling of the tertiary structure of an unusual plant cysteine proteinase. ananain (ec 3.4.22.31), from ananas comosus (pineapple) is distinguished from all other cysteine proteinases in the papain superfamily by having a unique combination of acidic amino acids. as well as lacking the acidic residue immediately preceding the active site histidine (position 158 in papain), it also lacks the extensive surface network of acidic residues ... | 2000 | 10998237 |
| continuous ethanol production from pineapple cannery waste using immobilized yeast cells. | the cells of saccharomyces cerevisiae atcc 24553, were immobilized in k-carrageenan and packed in a tapered glass column reactor for ethanol production from pineapple cannery waste at temperature 30 degrees c and ph 4.5. the maximum productivity was 42.8 g ethanol 1(-1) h(-1) at a dilution rate of 1.5 h(-1). the volumetric ethanol productivity of the immobilized cells was ca. 11.5 times higher than the free cells. the immobilized cell reactor was operated over a period of 87 days at a dilution r ... | 2000 | 10908799 |
| dietary serotonin and alcohol combined may provoke adverse physiological symptoms due to 5-hydroxytryptophol. | the urinary excretion products of serotonin (5-hydroxytryptamine, 5ht) are 5-hydroxyindole-3-acetic acid (5hiaa) and 5-hydroxytryptophol (5htol), and the ratio of 5htol to 5hiaa is normally very low (< 0.01 ) in man. intake of foods rich in 5ht (high amounts in banana, pineapple, and walnuts) induces a general increase in the output of 5ht metabolites, without affecting the 5htol/5hiaa ratio. in contrast, during metabolism of ethanol there is a shift in the catabolic pattern of 5ht, and the form ... | 2000 | 10968409 |
| evaluation of residual levels of benomyl, methyl parathion, diuron, and vamidothion in pineapple pulp and bagasse (smooth cayenne). | the objective of this research was to study the residual levels of benomyl, methyl parathion, diuron, and vamidothion in pineapple bagasse and pulp. benomyl (benlate), methyl parathion (folidol 600), diuron (krovar), and vamidothion (kilval 300) were applied pre-harvest to pineapples (smooth cayenne). after harvesting, the fruits were washed (100 ppm sodium hypochlorite) and the pulp was separated from the sub-products (peel, core, tops, and tails). the pulp was not submitted to any heat treatme ... | 2000 | 11087549 |
| [parasitic helminths from sigmodon hispidus (rodentia: cricetidae) from seasonal and evergreen habitats in costa rica]. | the helminthological fauna of the cotton rat, sigmodon hispidus in a tropical environment varies according to habitat and feeding behavior. six species of nematodes (longistriata adunca, trichostrongylus sigmodontis, strongyloides sigmodontis, litomosoides carinii, monodontus sp. and protospirura sp.) and two species of cestodes (hymenolepis diminuta and raillietina sp.) were found in rats from extensive dry lands in guanacaste where hot temperatures and heterogeneous diet are the rule. only two ... | 2000 | 11021315 |
| role of yersinia intermedia and pseudomonas putida in the development of a fruity off-flavour in pasteurized milk. | analysis by gas chromatography-mass spectrometry of pasteurized milk with a fruity (pineapple like) off odour and a sour, rancid and soapy taste indicated the presence of concentrations at microg/ml levels of ethyl butanoate, ethyl hexanoate, ethyl octanoate, ethyl decanoate, octanoic acid, decanoic acid and dodecanoic acid. the off-odour and taste were attributed to the presence of these compounds in the milk. microbiological examination confirmed that the milk was also contaminated with a seri ... | 2000 | 11131069 |
| molecular characterization of fusarium subglutinans associated with mango malformation. | abstract mango malformation is a serious disease of mangifera indica in tropical and subtropical regions of the world. this disease is caused by fusarium subglutinans, which is also associated with diseases on many other hosts, such as pineapple, pine, maize and sugarcane. the f. subglutinans strains associated with different hosts are virtually indistinguishable using morphological characters, but can be easily differentiated using histone and beta-tubulin gene sequencing, and some have subsequ ... | 2000 | 20572965 |
| prevalence and growth of pathogens on salad vegetables, fruits and sprouts. | a total of 120 samples, comprising different types of raw vegetables (seven), fruits (three) and sprouts (three) obtained from street vendors, were tested for aerobic plate count, coliform count and various food-borne pathogens. average aerobic plate counts for salad vegetables, fruits and sprouts were greater than 10(10) cfu/g and 10(9) cfu/g respectively. pathogens isolated were s. aureus, e. coli, enterobacter sp., klebsiella sp., s. typhi, serratia sp., providencia sp. and p. aeruginosa. the ... | 2001 | 11279816 |
| diuretic effects of selected thai indigenous medicinal plants in rats. | extracts of five indigenous thai medicinal having ethnomedical application in the treatment of dysuria were investigated for their diuretic activity. root extracts of ananas comosus and carica papaya, given orally to rats at a dose of 10 mg/kg, demonstrated significantly increased urine output (p < 0.01) which was 79 and 74%, respectively, of the effect of an equivalent dose of hydrochlorothiazide. both plant extracts gave similar profiles of urinary electrolyte excretion to that of the hydrochl ... | 2001 | 11297849 |
| ingestion of insoluble dietary fibre increased zinc and iron absorption and restored growth rate and zinc absorption suppressed by dietary phytate in rats. | we examined the effects of ingestion of five types of insoluble fibre on growth and zn absorption in rats fed a marginally zn-deficient diet (6.75 mg (0.103 mmol) zn/kg diet) with or without added sodium phytate (12.6 mmol/kg diet). the types of insoluble fibre tested were corn husks, watermelon skin, yam-bean root (pachyrhizus erosus) and pineapple core, and cellulose was used as a control (100 g/kg diet). body-weight gain in the cellulose groups was suppressed by 57 % by feeding phytate. body- ... | 2001 | 11591231 |
| comparison of culture media, simplate, and petrifilm for enumeration of yeasts and molds in food. | the efficacy of three culture media, dichloran rose bengal chloramphenicol (drbc), dichloran 18% glycerol agar (dg18), and potato dextrose agar (pda) supplemented with two antibiotics, were compared with the simplate and petrifilm techniques for mold and yeast enumeration. the following foods were analyzed: corn meal, wheat flour, cassava flour, bread crumbs, whole meal, sliced bread, ground peanuts, mozzarella cheese, grated parmesan cheese, cheese rolls, orange juice, pineapple pulp, pineapple ... | 2001 | 11601710 |
| small bowel review: normal physiology part 1. | in the past year there have been many advances in the area of small bowel physiology and pathology and therapy. in preparation for this review, over 1500 papers were assessed. the focus is on presenting clinically useful information for the practising gastroenterologist. selected important clinical learning points include the following: (1) glucose absorption mediated by sglt1 is controlled by mrna abundance, as well as by posttranscriptional processes including protein trafficking; (2) inducers ... | 2001 | 11768247 |
| evaluation of the antioxidant properties of mediterranean and tropical fruits compared with common food additives. | several mediterranean and tropical fruits have been analyzed in order to assess their antioxidant activity compared with that of common food additives (butylated hydroxyanisole [bha], butylated hydroxytoluene [bht] and propyl gallate). among mediterranean fruits, red grape and plum were more effective (p < 0.05) scavengers of peroxyl radicals than bha, bht, and propyl gallate. of the tropical fruits, banana was the most effective scavenger of peroxyl radicals. mediterranean and tropical fruits s ... | 2001 | 11770635 |
| bromelain reversibly inhibits invasive properties of glioma cells. | bromelain is an aqueous extract from pineapple stem that contains proteinases and exhibits pleiotropic therapeutic effects, i.e., antiedematous, antiinflammatory, antimetastatic, antithrombotic, and fibrinolytic activities. in this study, we tested bromelain's effects on glioma cells to assess whether bromelain could be a potential contributor to new antiinvasive strategies for gliomas. several complementary assays demonstrated that bromelain significantly and reversibly reduced glioma cell adhe ... | 2001 | 11774029 |
| an outbreak of campylobacter jejuni infections associated with food handler contamination: the use of pulsed-field gel electrophoresis. | in 1998, an outbreak of campylobacter jejuni infections occurred in kansas among persons attending a school luncheon; community cases were also reported. in a cohort study of luncheon attendees, 27 (17%) of 161 persons reported illness. consuming gravy (relative risk [rr], 4.2; 95% confidence interval [ci], 1.5-11.7) or pineapple (rr, 2.4; 95% ci, 1.0-5.7) was associated with illness. both foods were prepared in a kitchen that served 6 other schools where no illness was reported. a cafeteria wor ... | 2001 | 11078485 |
| nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2. | the genome of pineapple mealybug wilt-associated closterovirus-2 (pmwav-2) was cloned from double-stranded rna isolated from diseased pineapple and its sequence determined. the 3'-terminal 14861 nt of the single-stranded rna genome contains ten open reading frames (orfs) which, from 5' to 3', potentially encode a >204 kda polyprotein containing papain-like protease, methyltransferase and helicase domains (orf1a), a 65 kda rna-dependent rna polymerase (orf1b), a 5 kda hydrophobic protein (orf2), ... | 2001 | 11125151 |
| characterization of yeast strains for wine production: effect of fermentation variables on quality of wine produced. | sixteen yeast strains isolated from grapefruit (citrus paradis), orange (citrus sinensis) and pineapple (ananas comosus) were characterized using standard microbiological procedures. the species were identified as saccharomyces uvarum, s. cerevisiae, s. carlbergensis, and s. ellipsoideus. their abilities for wine production were tested by using sugar and ethanol tolerance tests. the best biochemically active strain, s. ellipsoideus, was used along with commercially available baker's yeast (s. ce ... | 2001 | 11732717 |
| antibody binding to venom carbohydrates is a frequent cause for double positivity to honeybee and yellow jacket venom in patients with stinging-insect allergy. | up to 50% of patients with stinging-insect allergy have double-positive rast results to honeybee and yellow jacket (yj) venom. true double sensitization and crossreactivity through venom hyaluronidases are considered main reasons for this multiple reactivity. | 2001 | 11742287 |
| 16s ribosomal dna characterization of nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril). | nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril) were characterized by amplified 16s ribosomal dna restriction analysis and 16s rrna sequence analysis. herbaspirillum seropedicae, herbaspirillum rubrisubalbicans, burkholderia brasilensis, and burkholderia tropicalis were identified. eight other types were placed in close proximity to these genera and other alpha and beta proteobacteria. | 2001 | 11319127 |
| a study on transfer factors of 60co and 65zn from soil to plants in the tropical environment of bangladesh. | soil to plant transfer factor (tf) of 60co and 65zn was determined from radioisotope experiments on plants grown in pots under outdoor ambient tropical conditions for three growing seasons (1995-1998). the tfs were obtained for different plants/crops such as, rice, bean, peanuts pineapple, cabbage, tomato, spinach and grass. the average tf values of 60co are found to be 0.087. 0.15, 0.12, 0.67, 0.28, 0.79, 1.03 and 0.34 respectively for the above mentioned plants/crops. in case of 65zn, the aver ... | 2001 | 11336414 |
| water quality of a reservoir as affected by agriculture in the east of thailand: a preliminary study. | a preliminary study on the water quality of a reservoir, affected by agriculture, in the east of thailand was conducted during 1996-1997. monitoring water quality of a reservoir is important because the sloping lands surrounding the reservoirs are mainly utilized for cultivating cash crops (pineapple, cassava, etc). a lot of fertilizers and agrochemicals were applied to soil and crops which can polluted the water. the results from the preliminary studied will be applied for the monitoring of the ... | 2001 | 11724479 |
| bromelain: biochemistry, pharmacology and medical use. | bromelain is a crude extract from the pineapple that contains, among other components, various closely related proteinases, demonstrating, in vitro and in vivo, antiedematous, antiinflammatory, antithrombotic and fibrinolytic activities. the active factors involved are biochemically characterized only in part. due to its efficacy after oral administration, its safety and lack of undesired side effects, bromelain has earned growing acceptance and compliance among patients as a phytotherapeutical ... | 2001 | 11577981 |
| comparison of two cysteine endopeptidases from pseudananas macrodontes (morr.) harms (bromeliaceae). | the properties of two cysteine peptidases (macrodontain i and ii) isolated from fruits of pseudananas macrodontes have been compared. the enzymes showed optimum ph ranges near neutrality and were inhibited by e-64 and other cysteine peptidase inhibitors. molecular masses were 23459 and 23703 kda, the isoelectric points were 6.1 and 5.9, and the km values were 13.4 and 8.9 microm (bz-phe-val-arg-amc) for macrodontain i and ii, respectively. n-alpha-cbz-l-amino acid p-nitrophenyl esters were teste ... | 2001 | 11517945 |
| quantification of l-ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis. | a standard curve for the quantification of l-ascorbic acid (l-aa) by capillary zone electrophoresis (cze) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, sunkist, and pineapple) and spinach were performed using d-isoascorbic acid (d-iaa) as an internal standard. the minimum detection limits (mdls) for l-aa and d-iaa were determined to be 1 and 2 microg/ml, respectively, at 265 nm. dehydroascorbic acid (dhaa) in fruits and spinach was quantified ... | 2001 | 11386660 |
| ferulic acid is esterified to glucuronoarabinoxylans in pineapple cell walls. | the ester-linkage of ferulic acid (mainly e) to polysaccharides in primary cell walls of pineapple fruit (ananas comosus) (bromeliaceae) was investigated by treating a cell-wall preparation with 'driselase' which contains a mixture of endo- and exo-glycanases, but no hydroxycinnamoyl esterase activity. the most abundant feruloyl oligosaccharide released was o-[5-o-(e-feruloyl)-alpha-l-arabinofuranosyl](1-->3)-o-beta-d-xylopyranosyl-(1-->4)-d-xylopyranose (faxx). this indicated that the ferulic a ... | 2001 | 11261585 |
| [rapid determination of ethephon residues in concentrated pineapple juice by head-space gas chromatography]. | the method developed is based on the special property that ethephon can be easily decomposed into ethene in alkaline solution by heating. certain amount of concentrated koh solution was added to the sample and heated at 70 degrees c. then, 1 ml of the gas above the sample was injected into a gas chromatograph by head-space sampler for the determination of the target compound with external standard quantitation method. the detection limit was 0.025 mg/kg and the fortified recoveries of ethephon i ... | 2001 | 12541822 |
| selection for aluminium tolerance in embryogenic lines of citrus sinensis l. and c. aurantium l. | the citrus cultivars 'amargo' and 'pineapple' produce embryogenic cultures on two types of sugars, galactose and lactose. two percent lactose is optimal for both cultivars; embryogenic callus is produced 8-10 weeks after inoculation. whereas 2% galactose is optimal for 'amargo', it is 3% for 'pineapple'. three types of embryos are recognized: whitish, greenish and heart shaped. in 8 to 10 week old cultures we do also observe a black spotted type of embryos. a high percentage of plant conversion ... | 2002 | 12491964 |
| preliminary comparison of bromelain and ibuprofen for delayed onset muscle soreness management. | the purpose of this study was to determine whether a common bromelain regimen or common ibuprofen regimen are effective in resolving pain and muscle dysfunction associated with delayed onset muscle soreness of the elbow flexors. | 2002 | 12466693 |
| pineapple juice as an agent for the digestion of fish prior to the harvesting of metacercariae. | the efficacy of crude pineapple juice obtained from ananus comosus in digesting fish for the harvesting of trematode metacercariae was investigated. no significant difference was found between the total number of metacercariae detected from fish (cirrhina jullieni) digested by acid pepsin and those digested by freshly prepared pineapple juice that was kept for 15 days at a temperature of either -4 degrees c or -75 degrees c. however, fewer metacercariae were found when using juice that had been ... | 2002 | 12971486 |
| movement of bromacil and hexazinone in soils of hawaiian pineapple fields. | bromacil and hexazinone have been heavily used to control weeds in the pineapple fields in central oahu, hawaii, usa, since 1970s and 1980s, respectively. the last application prior to this study was at a rate of 0.6 kg active ingredient (a.i.) ha(-1) for hexazinone and 1.8 or 3.4 kg (a.i.) ha(-1) for bromacil in the six study fields during june-october 1998. soils were collected from 0 to 1860 cm below the surface in january-may 1999 to survey the residue profiles of the two herbicides. stratop ... | 2002 | 12430654 |
| comparison of the nutrient content of fresh fruit juices vs commercial fruit juices. | to compare the types and quantities of carbohydrate, electrolytes, ph and osmolarity of fresh fruit juices and commercial fruit juices. | 2002 | 12403253 |
| 3d modeling and virtual endoscopy of the small bowel based on magnetic resonance imaging in patients with inflammatory bowel disease. | small bowel mri (mr imaging) is a new imaging modality that excellently depicts small intestine pathology in patients with inflammatory bowel disease. virtual endoscopy based on sectional imaging is a recently introduced technique to create endoscopy like views. the aim of this study was to evaluate the feasibility of virtual small bowel endoscopy based on mr imaging in patients with crohn disease. | 2002 | 12218449 |
| opalescent and cloudy fruit juices: formation and particle stability. | cloudy fruit juices, particularly from tropical fruit, are becoming a fast-growing part of the fruit juice sector. the classification of cloud as coarse and fine clouds by centrifugation and composition of cloud from apple, pineapple, orange, guava, and lemon juice are described. fine particulate is shown to be the true stable cloud and to contain considerable protein, carbohydrate, and lipid components. often, tannin is present as well. the fine cloud probably arises from cell membranes and app ... | 2002 | 12180775 |
| bromelain treatment alters leukocyte expression of cell surface molecules involved in cellular adhesion and activation. | bromelain is a natural proteinase preparation derived from pineapple stem that is marketed for oral use as a digestive aid and as an antiinflammatory agent. bromelain treatment in vitro has been previously shown to selectively remove certain cell surface molecules that may affect lymphocyte migration and activation. this study reports the effects of bromelain on a broad range of cell surface molecules and on lymphocytes, monocytes, and granulocytes under physiologically relevant conditions. in v ... | 2002 | 12165279 |
| identification and occurrence of the novel alkaloid pentahydroxypentyl-tetrahydro-beta-carboline-3-carboxylic acid as a tryptophan glycoconjugate in fruit juices and jams. | the novel carbohydrate-derived beta-carboline, 1-pentahydroxypentyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid, was identified in fruit- and vegetable-derived products such as juices, jams, and tomato sauces. this compound occurred as two diastereoisomers, a cis isomer (the major compound) and a trans isomer, ranging from undetectable amounts to 6.5 microg/g. grape, tomato, pineapple, and tropical juices exhibited the highest amount of this alkaloid (up to 3.8 mg/l), whereas apple, bana ... | 2002 | 12137498 |
| characterization of genomic sequence coding for bromelain inhibitors in pineapple and expression of its recombinant isoform. | bromelain inhibitor (bi) is a cysteine proteinase inhibitor isolated from pineapple stem (reddy, m. n., keim, p. s., heinrikson, r. l., and kézdy, f. j. (1975) j. biol. chem. 250, 1741-1750). it consists of eight isoinhibitors, and each isoinhibitor has a two-chain structure. in this study, the genomic dna has been cloned and found to encode a precursor protein with 246 amino acids (m(r) = approximately 27,500) containing three isoinhibitor domains (bi-iii, -vi, and -vii) that are 93% identical ... | 2002 | 12016215 |
| total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation. | epidemiological evidence links high intake of ascorbic acid (aa) and other antioxidant micronutrients to health promotion. it would be useful to know the overall, or 'total' antioxidant capacity of foods, to establish the contribution of aa to this, and to assess how this information may translate into dietary intakes to meet the new us daily reference intake for aa. in this study, the total antioxidant capacity, as the ferric reducing-antioxidant power (frap) value, and aa content of thirty-fou ... | 2002 | 11898770 |
| hydrogen isotopic profile in the characterization of sugars. influence of the metabolic pathway. | the site-specific natural hydrogen isotope ratios of plant metabolites determined by 2h nuclear magnetic resonance (snif-nmr method) can provide powerful criteria for inferring mechanistic and environmental effects on biosynthetic pathways. this work examines the potential of isotopic profiles for the main constituents of carbohydrates, glucose and fructose, to distinguish different photosynthetic pathways. an appropriate analytical strategy, involving three suitable isotopic probes, has been el ... | 2002 | 11879039 |