Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| pathogenicity of hirschmanniella oryzae, h. spinicaudata, and h. imamuri on rice. | in greenhouse experiments hirschmanniella oryzae, h. imamuri, and h. spinicaudata depressed and delayed the tillering and flowering of rice, and suppressed root and shoot growth and grain yield. | 1979 | 19305545 |
| repetitive dna in three gramineae species with low dna content. | the genomes of three gramineae species, namely finger millet (eleusine coracana), pearl millet (pennisetum americanum) and rice (oryza sativa) are characterized by studying their dna denaturation-reassociation properties. the reassociation kinetics measurement of the sonicated dna (500--700 nucleotide pairs) indicate the presence of a heterogeneous, repetitive dna fraction accounting for 49--54% of the total dna in all three species. from the cot 1/2 value of the slow reassociating dna, the geno ... | 1980 | 7409756 |
| lipid molecular species composition of thylakoid membranes. | lipid molecular species compositions of chloroplast thylakoid membranes of mesophyll cells from spinacia oleracea, glycine max, oryza sativa and zea mays and of bundle sheath cells from zea mays have been quantitatively determined. no significant difference in the lipid molecular species composition was found among the five membrane sources. the predominant molecular species of monogalactosyldiacylglycerol was the 1-linolenoyl parallel to 2-linolenoyl species. the 1-linolenoyl parallel to 2-lino ... | 1980 | 7188732 |
| purification and characterization of acid phosphatase in aleurone particles of rice grains. | the major acid phosphatase (ec 3.1.3.2) associated with aleurone particles of rice grains (oryza sativa l. japonica cv. koshihikari) was purified to homogeneous state by polyacrylamide gel electrophoresis. its molecular weight was 72,000 when determined by gel filtration and 68,000 when found by polyacrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate and β-mercaptoethanol. the purified enzyme had a violet color and an absorption peak at 530 nm. triton x-100 and lyso ... | 1980 | 25385961 |
| polysomes and intracisternal accumulations in enucleate sieve elements of rice (oryza sativa l.). | polysomes in sieve elements of rice (oryza sativa l.) were studied with the electron microscope. the polysomes were found on the rough endoplasmic reticulum (er) present in immature sieve elements and also on the cisternae of aggregated er in the parietal layer of mature, enucleate sieve elements. in the immature sieve elements the er cisternae existed as narrow profiles while in the mature sieve elements the er cisternae were considerably dilated and contained a fibrillar material and, occasion ... | 1980 | 24306690 |
| potentiality of leaf sheath cells for regeneration of rice (oryza sativa l.) plants. | regeneration of rice plantlets (oryza sativa l.) from calli originated from leaf sheath cells was made possible. this was possible in tissues initially grown in media containing 2.4-d (2.4-dichlorophenoxyacetic acid) at low temperature and illumination. the slow growing tissues were subsequently subjected to growth conditions at an elevated temperature and higher illumination with addition of kinetin and iaa and without 2.4-d. the suitability of leaf sheath cells for protoplast technology is ind ... | 1980 | 24301016 |
| influence of streptomycin on alpha-amylase formation isolated endosperms & protein pattern in intact seedlings of rice oryza sativa l. | 1980 | 6159319 | |
| effect of fluoride on in vivo nitrate reduction in the rive leaves (oryza sativa l.). | fluoride had no effect on in vitro nitrate reductase activity in rice leaves, but in vivo activity was strongly inhibited. it is suggested that fluoride brings about this inhibition by adversely affecting the physiological nadh generating system required for in vivo nitrate reduction. | 1980 | 7191373 |
| effects of toxic concentrations of nickel on growth & metabolism of rice (oryza sativa l.) seedlings. | 1980 | 7399618 | |
| promotion of mesocotyl growth in etiolated rice seedlings by 4-ethoxy-1-(p-tolyl)-s-triazine-2,6(1h,3h)-dione. | 4-ethoxy-1-(p-tolyl)-s-triazine-2,6(1h,3h)-dione (ta) promoted mesocotyl growth in dark-grown rice (oryza sativa l.) seedlings. in cultivars of the japonica type ta alone showed a small promotive effect and ta+gibberellic acid(ga3) had a marked synergistic effect, while in other cultivars, mostly of the indica type, ta alone showed a great promotive effect and ta+ga3 had only an additive effect. in cv. nato, a typical representative of cultivars showing the second type of response, the concentra ... | 1980 | 24311174 |
| effects of blue and red light on unrolling of rice leaves. | unrolling of the second leaf of 8-day-old rice (oryza sativa l.) seedlings was promoted by weak blue light (b), but not by red light (r). the effect of b was counteracted by irradiation with r just before or after the b. the counteracting effect of r was reversed by subsequent irradiation with far-red light but not by b, even if b was applied for 10 h. the b was effective when the region 0.5-2 cm from the tip of the leaf was irradiated. these results indicate that in rice photoreceptors for blue ... | 1980 | 24311163 |
| mechanism of monocarpic senescence in rice. | during grain formation stage (90 to 110 days), the youngest flag leaf of rice (oryza sativa l. cv. jaya) remained metabolically most active (as indicated by cellular constituents and enzyme activities) and the third leaf the least active. at the grain development stage (110 to 120 days) the above pattern of age-related senescence of the flag leaf completely changed and it senesced at a faster rate than the second leaf which remained metabolically active even up to grain maturation time (120 to 1 ... | 1980 | 16661186 |
| diallel analysis of heading date in rice (oryza sativa l.). | the genetics of heading date was investigated in an 8×8 diallel set of crosses involving diverse rice cultivars. wr, vr graph analysis revealed the presence of a complementary type of non-allelic interaction which apparently affected the position and slope of the regression line such as if there were overdominance. omission of two interacting parents resulted in a 6×6 subset of diallel crosses from which, as observed in the wr, vr graph, the non-allelic interaction had disappeared and the regres ... | 1980 | 24302365 |
| amino acid sequence of cytochrome c from rice. | the amino acid sequence of cytochrome c purified from rice, oryza sativa l., was determined. the complete amino acid sequence of rice cytochrome c is as follows: ac-ala-8-ser-phe-ser-glu-ala-pro-pro-gly1-asn-pro-lys-ala-gly-glu-lys-ile-phe10-lys-thr-lys-cys-ala-glx-cys-his-thr-val20-asp-lys-gly-ala-gly-his-lys-glx-gly-pro30-asx-leu-asx-gly-leu-phe-gly-arg-glx-ser40-gly-thr-thr-pro-gly-tyr-ser-tyr-ser-thr50-ala-asp-lys-asn-met-ala-val-ile-trp-glx60-glx-asx-thr-leu-tyr-asp-tyr-leu-leu-asn70-pro-tm ... | 1980 | 6244261 |
| growth and cell wall changes in rice coleoptiles : fractionation of cell wall and changes in noncellulosic polysaccharides during intact growth. | a fractionation of non-cellulosic sugars of oryza sativa l. coleoptile cell walls was carried out and the composition of each fraction was studied during coleoptile growth.percentages of fractions extracted with boiling water and with oxalate (pectic substances) were almost constant throughout development. an increase in the k ii hemicellulosic fraction (extracted with 24% koh) content, and a decrease in the k i hemicellulosic fraction (extracted with 10% koh) were detected, when coleoptile grow ... | 1981 | 24258865 |
| mobilization of metabolites from leaves to grains as the cause of monocarpic senescence in rice. | the pattern of senescence was studied by following the changes in chlorophyll and protein in the leaves and by measuring (32)p retention and export from source to sink during development of the rice plant (oryza sativa l. cv. jaya) subjected to different manipulative treatments. with the advance of reproductive development, the chronological sequence of leaf senescence was changed, so that the flag and the third leaf senesced earlier than did the second leaf. in presence of the daughter shoot of ... | 1981 | 16662105 |
| rapid growth and apparent total nitrogen increases in rice and corn plants following applications of triacontanol. | triacontanol (tria) increased fresh and dry weight and total reducible nitrogen (total n) of rice (oryza sativa l.) seedlings within 40 minutes. increases in total n in the supernatants from homogenates of corn (zea mays l.) and rice leaves treated with tria for one minute before grinding occurred within 30 and 80 minutes, respectively. the source for the increase was investigated utilizing atmospheric substitution and enrichment and depletion studies with (15)n. the increase in total n in seedl ... | 1981 | 16662092 |
| ontogenetic variation of nitrogenase, nitrate reductase, and glutamine synthetase activities in oryza sativa. | the relationship between the rates of nitrogenase, nitrate reductase, and glutamine synthetase activities, and plant ontogeny in rice (oryza sativa l.), cultivar ;m9', grown in salt marsh sediment with and without nitrate treatment was studied. in both treatments, nitrogenase activity measured as the immediate linear rate of acetylene reduction by bacteria associated with the roots varied with plant age. in control plants, the nitrogenase activity developed during the vegetative stage, peaked du ... | 1981 | 16661987 |
| transport of assimilates in the developing caryopsis of rice (oryza sativa l.) : the pathways of water and assimilated carbon. | the movement of water in the dorsal region of the developing rice caryopsis was studied using solutions of the heavy metals lanthanum and uranium. in the electron microscope electron-opaque deposits were confined to the cell walls of the pigment strand indicating that this is the main route for the water which enters and leaves the caryopsis during grain filling. the pathway of assimilates into the developing caryopsis was examined using isolated caryopses which had taken up solutions of fluores ... | 1981 | 24301111 |
| heterotrophic n(2) fixation and distribution of newly fixed nitrogen in a rice-flooded soil system. | rice (oryza sativa l.) plants growing in pots of flooded soil were exposed to a (15)n(2)-enriched atmosphere for 3 to 13 days in a gas-tight chamber. the floodwater and soil surface were shaded with a black cloth to reduce the activity of phototrophic n(2)-fixing micro-organisms. the highest (15)n enrichments were consistently observed in the roots, although the total quantity of (15)n incorporated into the soil was much greater. the rate of (15)n incorporation into roots was much higher at the ... | 1981 | 16661887 |
| transport of assimilates in the developing caryopsis of rice (oryza sativa l.) : ultrastructure of the pericarp vascular bundle and its connections with the aleurone layer. | assimilates entering the developing rice caryopsis traverse a short-distance pathway between the terminal sieve elements of the pericarp vascular bundle and the aleurone layer. the ultrastructure of this pathway has been studied. sieve elements in the pericarp vascular bundle are smaller than their companion cells.the sieve elements show few connections with surrounding vascular parenchyma elements but are connected to companion cells by compound plasmodesmata. companion cells, in turn, are conn ... | 1981 | 24302210 |
| intracellular distribution of enzymes associated with nitrogen assimilation in roots. | the cellular distribution of enzymes involved in nitrogen assimilation: nitrate reductase (ec 1.6.6.2), nitrite reductase (ec 1.6.6.4), glutamine synthetase (ec 6.3.1.2), glutamate synthase (ec 2.6.1.53), and glutamate dehydrogenase (ec 1.4.1.3) has been studied in the roots of five plants: maize (zea mays l. hybrid w 64a x w 182e), rice (oryza sativa l. cv. delta), bean (phaseolus vulgaris l. cv. contender), pea (pisum sativum l. cv. demi-nain), and barley (hordeum vulgare l.). initially, cell ... | 1981 | 24302111 |
| [microbiological determination of choline in rice (oryza sativa)]. | 1981 | 6982631 | |
| comparing time course profiles of immediate acetylene reduction by grasses and legumes. | the time course profiles of c(2)h(2) reduction by intact scirpus olneyi (bulrush), oryza sativa (rice) and spartina alterniflora (cordgrass) with roots in atmospheres of n(2) and 30-day-old glycine max (soybean) in air were all immediately linear. this is the first report of immediately linear rates of c(2)h(2) reduction by grass roots removed from soil. the immediately linear profile of c(2)h(2) reduction by soil-free grass roots was achieved by preventing contact between the roots and air. roo ... | 1981 | 16345684 |
| active polypeptide fragments common to prokaryotic, eukaryotic, and mitochondrial dna polymerases. | with a procedure that allows the renaturation of the dna polymerase catalytic activity in situ after sds-polyacrylamide gel electrophoresis, we have compared the active polypeptides present in extracts from organisms covering a wide evolutionary range from prokaryotes to eukaryotes, namely: escherichia coli, oryza sativa, daucus carota , neurospora crassa, dictyostelium discoideum, saccharomyces cerevisiae, ceratitis capitata, leucophaea maderae , xenopus laevis, rat tissues and human lymphoblas ... | 1982 | 6765191 |
| glutamate synthase isoforms in rice: immunological studies of enzymes in green leaf, etiolated leaf, and root tissues. | rabbit antiserum was raised against ferredoxin-dependent glutamate synthase (ec 1.4.7.1) purified from green leaves of oryza sativa l. cv delta. ferredoxin-dependent glutamate synthase, detected in green leaf, etiolated leaf, and root tissues cross-reacted completely with the antiferredoxin glutamate synthase immunoglobulin g. in contrast, the immunoglobulin g did not cross-react with nadh-dependent (ec 1.4.1.14) and nadph-dependent (ec 1.4.1.13) glutamate synthases found in nonphotosynthetic et ... | 1982 | 16662583 |
| heterosis breeding in rice (oryza sativa l.). | studies conducted at the international rice research institute (irri) during 1980 and 1981 have shown up to 73% heterosis, 59% heterobeltiosis and 34% standard heterosis for yield in rice. the latter was estimated in comparison to commercial varieties: ir36 and ir42 (yield 4-5 t/ha in wet season trials and 7-8 t/ha in dry season trials). generally speaking, absolute yield was lower and extent of standard heterosis was higher in wet season than in dry season with some exception. yields up to 5.9 ... | 1982 | 24270875 |
| embryogenesis in callus derived from rice microspores. | differentiating calli derived from rice (oryza sativa l.) microspores were examined histologically. shoot and root meristems were observed to be arising by both organogenesis as well as embryogenesis. embryoid attachment to callus (as well as other embryoids) was at the scutellum adjacent to the mesocotyl and radicle. these observations could be interpreted as an indication of the totipotent plasticity of that tissue. | 1982 | 24257769 |
| occurrence of nitrate reductase inhibitor in rice plants. | nitrate reductase inhibitor is usually found in the roots of rice plants (oryza sativa l. cv mr7), but it was also produced in the shoots of aging plants. the inhibitor was inducible in the shoot of rice seedlings by dark, minus-nitrate or plus-ammonium treatments. there appears to be a general involvement of the inhibitor in the control of nitrate assimilation in the plant. | 1982 | 16662758 |
| biosynthesis of storage proteins in developing rice seeds. | sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (oryza sativa l. japonica cv koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the components of glutelin, the major storage protein in rice seed, appeared 5 days after flowering. a 26-kilodalton polypeptide, the globulin component, also appeared 5 days after flow ... | 1982 | 16662620 |
| light inhibition of the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene in leaves is mediated through carbon dioxide. | the mechanism of light-inhibited ethylene production in excised rice (oryza sativa l.) and tobacco (nicotiana tabacum l.) leaves was examined. in segments of rice leaves light substantially inhibited the endogenous ethylene production, but when co2 was added into the incubation flask, the rate of endogenous ethylene production in the light increased markedly, to a level which was even higher than that produced in the dark. carbon dioxide, however, had no appreciable effect of leaf segments incub ... | 1982 | 24271776 |
| mechanical properties of the rice panicle. | curvature, bending moment, and second moment of stem cross-sectional area were evaluated from photographic data and used to compute flexural rigidity and young's modulus in the panicle rachis of rice, oryza sativa l. ;m-101.' flexural rigidity c, and its components e, young's modulus, and i, the moment of inertia of the area about the neutral axis, were evaluated 1.5 cm (tip), 9.5 cm (mid), and 16.5 cm (base) from the tip of the panicle rachis. in dynes per square centimeter, c increases from 1. ... | 1982 | 16662516 |
| enzymic mechanism of starch breakdown in germinating rice seeds : 12. biosynthesis of alpha-amylase in relation to protein glycosylation. | the biosynthetic mechanism of alpha-amylase synthesis in germinating rice (oryza sativa l. cv. kimmazé) seeds has been studied both in vitro and in vivo. special attention has been focused on the glycosylation of the enzyme molecule. tunicamycin was found to inhibit glycosylation of alpha-amylase by 98% without significant inhibition of enzyme secretion. the inhibitory effect exerted by the antibiotic on glycosylation did not significantly alter enzyme activity.in an in vitro system using poly-( ... | 1982 | 16662436 |
| response of excised embryos of rice (oryza sativa l.) to x-rays. | the response of rice (oryza sativa l.) embryos to x-rays (m1 to m3) was studied. by means of irradiating excised embryos, both chlorophyll and macromutation were successfully induced in three genotypes of rice. however, differential responses in terms of mutation frequency, mutation spectrum and optimal levels of x-rays required for induction of mutation (chlorophyll as well as morphological) were found to exist between cultivars. in 'satika' and 'ashkhata', ld50 values and maximum induced seed ... | 1982 | 24270335 |
| senescence of rice leaves : vii. proline accumulation in senescing excised leaves. | proline content increased greatly in detached rice (oryza sativa cv. taichung native 1) leaves during senescence. there was a slight but significant increase in proline level after one day of incubation, and, subsequently, proline accumulated relatively rapidly. by 4 days after excision, the level of proline had increased 30- to 50-fold, which is similar to the level seen in the water-stressed detached rice leaves. it is unlikely that the proline accumulation in detached leaves is to be derived ... | 1982 | 16662400 |
| physiology of root-associated nitrogenase activity in oryza sativa. | an intact method for measuring immediately linear rates of acetylene reduction was used to investigate the relationship between temperature, ph, o(2) concentration, and light intensity with the rate of root-associated nitrogenase activity in rice (oryza sativa l.). nitrogenase activity varied over a temperature range of 10 to 50 degrees c and optimal rates of acetylene reduction were recorded at 35 degrees c. nitrogenase activity was also influenced by the ph of the liquid surrounding the roots ... | 1982 | 16662363 |
| glutamate synthase from rice leaves. | ferredoxin-dependent glutamate synthase (ec 1.4.7.1) from rice leaves (oryza sativa l. cv delta) was purified 206-fold with a final specific activity of 35.9 mumoles glutamate formed per min per milligram protein by a procedure including ammonium sulfate fractionation, deae-cellulose chromatography, sephacryl s-300 gel filtration, and ferredoxin-sepharose affinity chromatography. the purified enzyme yielded a single protein band on polyacrylamide gel electrophoresis. molecular weight of the nati ... | 1982 | 16662307 |
| rice protein-body formation: all types are initiated by dilation of the endoplasmic reticulum. | the ultrastructure of protein deposition in the starchy endosperm of developing rice (oryza sativa l.) grains was examined in conventionally fixed (glutaraldehyde and osmium tetroxide) tissues and also in thick sections (0.3 μm) of zinc iodide-osmium tetroxide post-fixed tissue. three types of previously characterised protein body were observed and it was shown that each type was initiated by dilations of the endoplasmic reticulum. crystalline type protein bodies were initiated by a ribosome-fre ... | 1982 | 24275981 |
| studies on protein characteristics in rice mutants. | twenty mutants isolated from "latisail", "jhingasail" and "pankaj" varieties of rice (oryza sativa l.) were screened for two aspects of nutritive quality, namely crude protein content and distribution pattern of protein in the endosperm. observations revealed a wide variation for both characters, and while there was no consistent association between protein content and test grain weight, which varied between varieties, a positive correlation between protein content and grain sterility was noted. ... | 1982 | 24271369 |
| genetic analysis of resistance to whitebacked planthopper in twenty-one varieties of rice, oryza sativa l. | the inheritance of resistance to whitebacked planthopper sogatella furcifera (horvath) was studied in 21 rice varieties. reactions of f1; f2 and f3 progenies of the crosses of 21 resistant varieties with the susceptible variety 'tn 1' revealed that a single dominant gene governs resistance in 'mushkan 41', 'santhi', 'siahnakidar 195', 'sm2-34', 'tirisurkh 251', 'zirijowaian 245', '18', '24a', '39', '76 s', '78', '180', '213 b', '267', '293', 'ci 6037-4', 'np97', 's39 jkw' and 'bansphul'. in vari ... | 1982 | 24271368 |
| lectin accumulation and synthesis in developing rice embryos. | rice (oryza sativa, l.) lectin is specifically synthesized during seed formation. it accumulates very rapidly between 8 and 16 days post anthesis and reaches its final content 20 days post anthesis. the rate of synthesis of this protein dramatically increases during the developmental phase coinciding with the rapid lectin accumulation. when the lectin accumulation stops, its rate of synthesis declines rapidly and eventually stops in fully mature embryos. it appears, therefore, that rice lectin i ... | 1983 | 24258184 |
| photosynthesis and ribulose 1,5-bisphosphate carboxylase in rice leaves: changes in photosynthesis and enzymes involved in carbon assimilation from leaf development through senescence. | changes in photosynthesis and the ribulose 1,5-bisphosphate (rubp) carboxylase level were examined in the 12th leaf blades of rice (oryza sativa l.) grown under different n levels. photosynthesis was determined using an open infrared gas analysis system. the level of rubp carboxylase was measured by rocket immunoelectrophoresis. these changes were followed with respect to changes in the activities of rubp carboxylase, ribulose 5-phosphate kinase, nadp-glyceraldehyde 3-phosphate dehydrogenase, an ... | 1983 | 16663318 |
| somaclonal genetics of rice, oryza sativa l. | the inheritance and variations of some traits of more than 2,000 somatic cell derived plants of rice (oryza sativa l.) were investigated in the second and third generations (t2 and t3) of regenerated plants (somaclones). the percentages of multiploids occurring in somaclones ranged from 0-13.3 in nine varieties (or hybrids) of 'hsien' (indica) group, but no multiploid was found in nine varieties (or hybrid) of 'keng' (japonica) group. a dwarf mutant with the height of only 20 cm was isolated in ... | 1983 | 24258482 |
| seed dormancy in red rice : iii. response to nitrite, nitrate, and ammonium ions. | sodium nitrite at 10 millimolar breaks dormancy of dehulled red rice (oryza sativa). while germination is light independent, low ph conditions (ph 3) are required for maximum response. water and buffer controls at ph 3 remain dormant. the response to nitrite occurs at 25 and 30 degrees c but is reduced at 20 degrees c, although nondormant seeds germinate readily at this temperature. the contact time for response to nitrite is less than 2 h at the start of imbibition. seeds imbibed first in water ... | 1983 | 16663224 |
| tryptophan enhancement of somatic embryogenesis in rice. | cereal embryos can produce two types of callus. one type, termed "embryogenic," consists of small meristematic-like cells and gives rise to many plants by somatic embryogenesis if placed on a suitable regeneration medium. the other is termed "nonembryogenic" and consists of long tubular cells which gives rise to few or no plants. high concentrations of tryptophan increased the formation of embryogenic callus in three rice cultivars (oryza sativa l. calrose 76, pokkali, and ir 36) but not in four ... | 1983 | 16663163 |
| cell-cycle dependency of radiosensitivity and mutagenesis in fertilized egg cells of rice, oryza sativa l. : 1. autoradiographic determination of the first dna synthetic phase. | to determine the time and duration of the first and second dna synthetic phases in fertilized egg cells and central cells of rice, a total of 753 ovules were sampled at 2 h intervals during the first 30 h after pollination and exposed to (3)h-thymidine for 2 h at 25 °c. autoradiographic observation of labeled nuclei was made for fertilized egg cells, as well as for central and antipodal cells. the first and second dna synthetic phases in fertilized egg cells were found 8-12 h and 21-25 h after p ... | 1983 | 24263540 |
| the role of ethylene in the growth response of submerged deep water rice. | we investigated the effect of partial submergence on internode elongation in a bangladesh variety of floating or deep water rice (oryza sativa l., cv. habiganj aman ii). in plants which were at least 21 days old, 7 days of submergence led to a 3- to 5-fold increase in internodal length. during submergence, the ethylene concentration in the internodes increased from about 0.02 to 1 microliters per liter. treatment of nonsubmerged plants with ethylene also stimulated internode elongation. when eth ... | 1983 | 16663022 |
| subcellular localization of rice leaf aryl acylamidase activity. | the intracellular localization of aryl acylamidase (aryl-acylamide amidohydrolase, ec 3.5.1.13) in rice (oryza sativa l. var starbonnet) leaves was investigated. the enzyme hydrolyzes and detoxifies the herbicide propanil (3,4-dichloropropionanilide) thereby accounting for immunity of the rice plant to herbicidal action. fractionation of mesophyll protoplasts by differential centrifugation yielded the highest specific activity of amidase in the crude mitochondrial fraction. further separation of ... | 1983 | 16662987 |
| adaptation of ribonucleic acid metabolism to anoxia in rice embryos. | rice (oryza sativa var. cigalon) is a plant which can adapt to very stringent anoxic conditions. it has previously been shown that during the adaptation period the energy charge can be used as a marker for metabolic activity. we have studied rna metabolism during this period and correlated it with changes in the energy charge. uptake of labeled precursor, utp-specific activity, and incorporation were measured. immediately after transfer to anaerobic conditions, the utp pool size is reduced and t ... | 1983 | 16662943 |
| alcohol dehydrogenase and an inactivator from rice seedlings. | alcohol dehydrogenase (adh) was measured in the various organs of rice seedlings (oryza sativa) growing in air. in extracts from ungerminated seeds, the adh is stable, but in extracts from seedlings more than 2 days old the enzyme initially present loses activity in a time- and temperature-dependent fashion, due to the presence of an inactivating component which increases with age in roots and shoots. the inactivation can be prevented completely by dithiothreitol, and when this is included in th ... | 1983 | 16662898 |
| enzymic mechanism of starch breakdown in germinating rice seeds : 15. immunochemical study on multiple forms of amylase. | the formation of multiple forms of amylases in germinating rice (oryza sativa l. cv kimmaze) grains was examined by means of isoelectric focusing, cross-immunoelectrophoresis, and rocket-line immunoelectrophoresis followed by a reaction of enzymic characterization by using beta-limit dextrin or starch as substrate. the constituents detected by isoelectric focusing were identified as three electrophoretically heterogeneous antigens. the major alpha-amylase bands a and b corresponded to a same ant ... | 1983 | 16662805 |
| alcohol dehydrogenase inactivator from rice seedlings : properties and intracellular location. | the alcohol dehydrogenase (adh) inactivator from aerobically grown rice (oryza sativa) coleoptiles was shown to be associated with membranes which were recovered in sucrose gradients at peak density 1.13 grams per cubic centimeter. when mg(2+) was included in the gradient, the inactivator was recovered at peak density 1.16 grams per cubic centimeter coinciding with the marker enzyme for endoplasmic reticulum, antimycin a-insensitive nadh cytochrome c reductase. adh was recovered exclusively in c ... | 1983 | 16662899 |
| [evaluation of nutritional potential of fish in diets based on beans (phaseolus vulgaris) and a cereal/corn (zea mays) and/or rice (oryza sativa)]. | p6e complementation between corn and fish, and rice and fish was evaluated for the purpose of establishing the required levels of each constituent in the mixture to obtain the optimum value in the biological evaluations for each diet. the optimum fish levels were around 10 and 6% for the corn and rice diets, respectively. complementary levels of fish in the bean: corn and bean:rice diets were evaluated in the same manner. the best values in the biological evaluation were obtained with only 2% of ... | 1983 | 6675550 |
| somatic embryogenesis and plant regeneration in an interspecific hybrid of oryza. | an embryogenic callus was obtained from immature panicle of an interspecific hybrid (oryza sativa x o. latifolia) f1. the medium consisted of he salts supplemented with 2,4-d, naa (each 2 mg/l), kinetin (3 mg/l), yeast extract (1360 mg/l) and casein hydrolyzate (300 mg/l). the callus was milk-white in colour compact and granulate in texture. various developmental stage of embryoid, such as globular, heart-shape, scutellum-shape and mature embryoid were observed in an embryogenic callus. plantlet ... | 1983 | 24258042 |
| inactivation of aphidicolin by plant cells. | plant cells are endowed with an aphidicolin inactivating activity. data on cultured cells show that the rate of inactivation depends on the cell type, daucus carota cells being the most effective among the other tested materials (oryza sativa and nicotiana plumbaginifolia). also germinating seedling of haplopappus gracilis and of citrullus vulgaris inactivate aphidicolin.inactivation, which may lead to unexpected results when a prolonged incubation with the drug is required, as in the case of th ... | 1983 | 24258125 |
| effect of sesbania rostrata on hirschmanniella oryzae in flooded rice. | microplot experiments on flooded soil infested with hirschmanniella oryzae were conducted to investigate the influence of the legum sesbania rostrata as a rotation crop with rice, oryza sativa l. cv. moroberekan. to avoid a green manure effect from s. rostrata, all aerial parts were removed at harvest. the dry weight of paddy, culms and leaves, and number of culms of rice following sesbania were 214%, 158%, and 121% greater, respectively, than those following rice. ripening of the paddy occurred ... | 1983 | 19295801 |
| physiology and bio - chemistry of germination of different types of seeds - iv - effect of certain chemicals on growth anddevelopment of cucumber, mungo, paddy, raddish and tomato plants. | the effect of 100 ppm solution of each of kinetin, adenine, uracil and thymine on the vegetative and reproductive growth of cucumis sativus, phaseolus mungo, oryza sativus, raphanus sativus and lycopersicum esculentum plants were studied. the rate of vegetative growth was found to be more in the cucumis sativus, raphanus sativus and lycopersicum esculentum plants treated with all the chemicals mentioned above over that of the controls. phaseolus mungo and oryza sativa plants shows almost the sam ... | 1984 | 22557413 |
| biological changes observed on rice and biological and genetic changes observed on tobacco [correction of tabacco] after space flight in the orbital station salyut-7 (biobloc iii experiment). | caryopses and isolated embryos from rice (oryza sativa l.) and tobacco seeds (nicotiana tabacum l. variety xanthi) were studied in the biobloc iii container aboard the soviet orbital space station salyut 7. the recovery from radiation damage under conditions of space flight was observed for rice caryopsis and embryos gamma irradiated (co 60, 50 grays) prior to launch. there was a large decrease in the percentage of germinating seeds from the tobacco strain tested when the seeds were exposed to h ... | 1984 | 11539650 |
| occurrence of aphelenchoides besseyi in louisiana rice seed and its interaction with sclerotium oryzae in selected cultivars. | aphelenchoides besseyi, the nematode causal agent of white-tip disease of rice, was recovered from 5.5% of 474 seed samples obtained from rice seed warehouses in louisiana. laboratory tests in which a. besseyi-infested rice seed was treated with phostoxin(r), a compound used for control of insects in stored grain, indicate that it also has nematicidal properties. in 18-week-duration greenhouse tests, populations of a. besseyi increased 4-5-fold on the cultivars saturn and melrose and 3-fold on n ... | 1984 | 19295876 |
| effect of submergence on translocation, starch content and amylolytic activity in deep-water rice. | submergence induces rapid internodal elongation in deep-water rice (oryza sativa l. cv. habiganj aman ii). we investigated the metabolic activities which help to support such fast growth. three days of submergence in water under continuous light led to the mobilization of 65% of the starch from those regions of rice internodes which had been formed prior to submergence. disappearance of starch was accompanied by a 70-fold enhancement of amylolytic activity. similar increases in amylolytic activi ... | 1984 | 24253273 |
| role of gibberellin in the growth response of submerged deep water rice. | we have shown previously that ethylene, which accumulates in the air spaces of submerged stem sections of rice (oryza sativa l. cv "habiganj aman ii"), is involved in regulating the growth response caused by submergence. the role of gibberellins in the submergence response was studied using tetcyclacis (tcy), a new plant growth retardant, which inhibits gibberellin biosynthesis. stem sections excised from plants that had been watered with a solution of 1 micromolar tcy for 7 to 10 days did not e ... | 1984 | 16663977 |
| genetic control of meiosis in rice, oryza sativa l. iii. effect of ds genes on genetic recombination. | the recombination frequency as influenced by five independent recessive ds genes was measured on three segments of different chromosomes of rice, oryza sativa l. each ds gene in the homozygous condition resulted in an almost equally reduced recombination frequency in the three segments. when the mean reduction in recombination frequency was related to the reduction of chiasma frequency, the five ds genes were divided into two types: in one type the reduction of chiasma frequency almost correspon ... | 1984 | 17246240 |
| cytochrome oxidase subunit ii gene of rice has an insertion sequence within the intron. | we have isolated and sequenced the cytochrome oxidase subunit ii gene from rice (oryza sativa l. var labelle). the overall structural organization of this gene is very similar to that of the maize gene. this gene contains an intron in a position identical to the intron in the maize gene. however, the intron in the rice gene is longer than that of the maize gene largely due to a 461 bp insertion sequence, which has inverted repeats at its termini and is flanked by direct repeats, characteristic o ... | 1984 | 6093039 |
| hydrolysis of conjugated gibberellins by β-glucosidases from dwarf rice (oryza sativa l. cv. «tan-ginbozu»). | dwarf rice (oryza sativa l. cv. «tan-ginbozu») is widely used for gibberellin conjugate bioassay. soluble and particulate fractions from seeds and seedlings of this variety showed hydrolytic activity toward [(3)h]ga-o-glc. the soluble fraction from mature seeds exhibited the highest [(3)h]ga-o-glc/pnp-β-glc hydrolysis ratio which decreased during the early germination phase. the soluble β-glucosidases from mature seeds were efficiently separated by cmsephadex c-50 column chromatography. it was f ... | 1984 | 23195047 |
| effect of submergence on the cell wall composition of deep-water rice internodes. | the cell wall composition of internodes of deep-water rice plants (oryza sativa l. cv habiganj aman ii) which were induced to grow rapidly by submergence in water was compared to that of nonsubmerged plants which grew slowly. no differences could be detected in cellulose, uronic acid, and lignin content expressed on a dry weight basis. cell wall preparations of rapidly growing, submerged internodes contained more hydroxyproline and had a higher hydration capacity than those of control internodes ... | 1984 | 16663779 |
| subcellular site of lectin synthesis in developing rice embryos. | embryos of developing rice (oryza sativa l. cv. koshihikari) caryopses which actively synthesize lectin were labelled with [s]cysteine for different times and newly synthesized rice lectin was isolated by affinity chromatography. gel filtration of embryo extracts on sepharose-4b indicated that a large portion of the labelled lectin was associated with the particulate fraction. experiments with detergent indicated that this lectin was sequestered within organelles. when extracts of pulse-labelled ... | 1984 | 16453545 |
| cell-cycle dependency of radiosensitivity and mutagenesis in fertilized egg cells of rice, oryza sativa l. : 2. x-ray sensitivity and mutation rate during a cell cycle. | in order to examine changes in survival and mutation rates during a cell cycle in higher plant, fertilized egg cells of rice were irradiated with x-rays at 2 h intervals for the first 36 h after pollination, i.e., at different phases of the first and second cell cycles. the most sensitive phase in lethality was late g1 to early s, followed by late g2 to m, which were more sensitive than the other phases. in both m1 and m2 generations, sterile plants appeared most frequently when fertilized egg c ... | 1984 | 24257637 |
| influence of osmotic adjustment on leaf rolling and tissue death in rice (oryza sativa l.). | osmotic adjustment, measured by the lowering of the osmotic potential at full turgor, and its influence on leaf rolling and leaf death was assessed in the lowland rice (oryza sativa l.) cultivar ir36 in both the greenhouse and field. the degree of osmotic adjustment varied with the degree and duration of stress, but was usually 0.5 to 0.6 megapascal (maximally 0.8 to 0.9 megapascal) under severe stress conditions. in leaves in which osmotic adjustment was 0.5 to 0.6 megapascal, leaf rolling and ... | 1984 | 16663622 |
| nonosmotic effects of polyethylene glycols upon sodium transport and sodium-potassium selectivity by rice roots. | addition of polyethylene glycol (peg) as an osmotic agent (at -230 kilopascals) dramatically lessened the toxicity of nacl (at 50 moles per cubic meter) to rice (oryza sativa l.) seedlings. this was explained by a reduction in the uptake of nacl. this reduction was much greater than could be accounted for by the lowered transpiration rate resulting from the solute potential changes due to the peg.low concentrations of peg (-33 kilopascals and less) had no effect upon transpiration rate but reduc ... | 1984 | 16663615 |
| primary trisomics of rice: origin, morphology, cytology and use in linkage mapping. | twelve primary trisomics of oryza sativa l. were isolated from the progenies of spontaneous triploids and were transferred by backcrossing to the genetic background of ir36, a widely grown high yielding rice variety. eleven trisomics can be identified morphologically from one another and from diploids. however, triplo 11 is difficult to distinguish from diploid sibs.-the extra chromosome of each trisomic was identified cytologically at pachytene stage of meiosis, and the chromosomes were numbere ... | 1984 | 17246212 |
| possible roles of calcium and calmodulin in the biosynthesis and secretion of alpha-amylase in rice seed scutellar epithelium. | the scutellar epithelial cells of rice (oryza sativa l. cv kimmazé) seeds actively secrete alpha-amylase in an early stage of germination. employing an in vivo system of freshly dissected scutellar tissues, effect of ca(2+) on the biosynthesis and the secretion of alpha-amylase have been studied. the maximum biosynthetic rate was saturated at about 0.5 mm external ca(2+) concentrations, whereas the secretion continued to increase to concentrations above 10 mm ca(2+). in the presence of 1 mm ca(2 ... | 1984 | 16663573 |
| abscisic acid promotes lectin biosynthesis in developing and germinating rice embryos. | immature rice (oryza sativa, l) embryos isolated about 12 days post anthesis are fully able to develop into young seedlings when cultured in vitro. concomitantly, they rapidly loose their lectin synthesis activity. abscisic acid added to the nutrient medium prevents precocious germination of the immature embryos and simultaneously strongly promotes lectin biosynthesis activity. similarly, abscisic acid keeps mature embryos grown in a nutrient medium in a dormant state and maintains their lectin ... | 1984 | 24253393 |
| the cellular basis of the elongation response in submerged deep-water rice. | the cellular basis of internode elongation was studied in intact deep-water rice plants (oryza sativa l. cv. "habiganj aman ii") and in isolated stem sections. in intact plants, growth was stimulated by submergence in water and by ethylene treatment. in isolated sections, growth was enhanced by submergence, by ethylene, and by exposure of the tissue to an atmosphere of 3% o2, 91% n2 and 6% co2 or 3% o2, 91% n2, 6% co2 and 1 μl l(-1) c2h4 (by vol.). under all these conditions, growth was localize ... | 1984 | 24258374 |
| regulation of growth in stem sections of deep-water rice. | submergence in water greatly stimulates internodal elongation in excised stem sections of deep-water rice (oryza sativa l. cv. "habiganj aman ii") and inhibits growth of leaf blades and leaf sheaths. the highest rates of internodal growth have been observed in continuous light. very little growth occurs in submerged sections kept in darkness or incubated under n2 in the light. the effect of submergence on the growth of deep-water rice is, at least in part, mediated by c2h4, which accumulates in ... | 1984 | 24258373 |
| identification and localization of sugar components of rice (oryza sativa l.) root cap mucilage. | this study is devoted to the neutral sugar analysis of rice root exudate and root cap mucilage.the results indicate that most of the carbohydrate in the exudate is released in soluble dialysable form, mainly as glucose. mucilage polymers represent only a minor fraction of the whole exudate containing glucose, galactose, xylose, arabinose, and minor amounts of fucose and mannose. in situ localization of some of these sugar residues is also reported. the strongest reaction detected is with fucose- ... | 1984 | 23195302 |
| cytogenetical analysis on aneuploids obtained from pollenclones of rice (oryza sativa l.). | rice aneuploids were obtained from 1,715 pollenclones with a mean frequency of 10.2% in anther culture (1983 to 1985). among the aneuploids obtained, the frequency of primary trisomics ranged from 5.4% to 6,7%, tetrasomics from 1.1% to 1.7% monosomics from 0.9% to 1.3%, nullisomics from 0.5% to 1% and double trisomics from 0.5% to 0.7%. the chromosome complements of those aneuploids were identified by pachytene analysis on the absolute length of the extra chromosomes. pollen clonal aneuploids sh ... | 1985 | 24247462 |
| photosynthesis and ribulose-1,5-bisphosphate carboxylase/oxygenase in rice leaves from emergence through senescence. quantitative analysis by carboxylation/oxygenation and regeneration of ribulose 1,5-bisphosphate. | changes in gas-exchange rates during the life span of the leaves of rice (oryza sativa l.) were analyzed quantitatively by measuring changes in the carboxylation/oxygenation and regeneration of ribulose 1,5-bisphosphate (rubp) at photon fluence rates of 2000 (saturating) and 500 (subsaturating) μmol quanta·m(-2)·s(-1) under ambient air conditions. the rubp levels were always higher than the active-site concentrations of rubp carboxylase (ec 4.1.1.39), irrespective of the irradiance supplied. ana ... | 1985 | 24241526 |
| genetic divergence among some maintainer and restorer lines in relation to hybrid breeding in rice (oryza sativa l.). | a representative group of 100 elite lines, 67 of which are restorers and 33 maintainers, from 68 crosses made at irri and 18 improved varieties from five countries were studied, using mahalonobis, d(2)-statistic and canonical analysis, to understand the nature and magnitude of divergence and to assess the importance of a set of quantitative characters related to yield in genetic differentiation. the 100 genotypes were grouped into 13 clusters. there were three single variety clusters and the num ... | 1985 | 24253127 |
| enzymic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase purified from rice leaves. | the enzymic properties of ribulose 1,5-bisphosphate (rubp) carboxylase/oxygenase purified from rice (oryza sativa l.) leaves were studied. rice rubpcarboxylase, activated by preincubation with co(2) and mg(2+) like other higher plant carboxylases, had an activation equilibrium constant (k(c)k(mg)) of 1.90 x 10(5) to 2.41 x 10(5) micromolar(2) (ph 8.2 and 25 degrees c). kinetic parameters of carboxylation and oxygenation catalyzed by the completely activated enzyme were examined at 25 degrees c a ... | 1985 | 16664401 |
| genetic variations in the hybrids of rice (oryza sativa) and sorghum (sorghum vulgare). | some of results from morphological and cytological observations and esterase-isozyme studies of a rice-sorghum hybrid are presented in this paper. there is a great diversity of morphological characters and some special characteristics in the progenies of the hybrids of rice with sorghum. the meiosis of pollen mother cells in the early generations of the hybrid was found to be abnormal. one main band coinciding with one found in sorghum but lacking in rice appeared in the majority of the hybrid l ... | 1985 | 24253065 |
| diallel analysis of submergence tolerance in rice, oryza sativa l. | the genetics of submergence tolerance in rice was studied in a 10 × 10 half-diallel cross set involving 10 lowland rice varieties, four of which were tolerant ('fr13a', 'fr43b', 'kurkaruppan', and 'goda heenati') and the remaining six were nontolerant ('rd19', 'ir42', 'ir17494-32-1', 'ir19672-24-3', 'jagannath', and 'cr1009'). estimates of genetic parameters following hayman's method showed significant additive and nonadditive gene action and the latter appeared to be solely due to dominance. na ... | 1985 | 24253055 |
| interaction of rice (oryza sativa) lectin with n-acetylglucosaminides. fluorescence studies. | the interaction of lectin isolated from rice (oryza sativa) embryos with n-acetylglucosaminides was studied by equilibrium dialysis and fluorescence. equilibrium dialysis with 4-methylumbelliferyl-(glcnac)2 showed that rice lectin (mr 38000) contains four equivalent saccharide-binding sites. addition of the n-acetylglucosaminides glcnac, (glcnac)2 and (glcnac)3 enhanced the intrinsic fluorescence of rice lectin and this was accompanied by a 10nm blue-shift of its maximum fluorescence with (glcna ... | 1985 | 4052018 |
| photosynthetic development of anaerobically grown rice (oryza sativa) after exposure to air. | during anaerobic germination, rice produces a coleoptile devoid of carotenoid and chlorophyll. further development and greening of the shoot occur upon exposure of the seedlings to air. in this study, a comparison was made between anaerobically (n(2)) germinated rice, greened upon exposure to air, and air/dark (a/d) germinated seedlings, greened upon exposure to light. after exposure to air, n(2)-grown seedlings had a 76-hour lag before net oxygen evolution occurred compared to a 6-hour lag for ... | 1985 | 16664275 |
| interrelationship of cultivated rices oryza sativa and o. glaberrima with wild o. perennis complex : analysis of fraction 1 protein and some repeated dna sequences. | phylogenetic relationship of the cultivated rices oryza sativa and o. glaberrima with the o. perennis complex, distributed on the three continents of asia, africa and america, and o. australiensis has been studied using fraction 1 protein and two repeated dna sequences as markers. fraction 1 protein isolated from the leaf tissue of accessions of different species was subjected to isoelectric focusing. all the species studied have similar nuclear-encoded small subunit polypeptides and chloroplast ... | 1985 | 24254178 |
| inheritance of callus formation ability in anther cultures of rice, oryza sativa l. | inheritance of ability to form callus in rice anther culture was studied using the diallel technique. anthers containing uninucleate microspores from two japonica cultivais ('minehikari' and 'taipei 309'), two indica cultivars ('mingolo' and 'suweon 290'), and 12 f1's of the diallel crosses involving these four parents were cultured on chaleffs r2 medium and evaluated for callus induction. the parents showed significant differences in anther callus formation, from 41.9% ('taipei 309') to 0% ('su ... | 1985 | 24254166 |
| effect of zinc deficiency on kinetics of ribulose-1,5-biphosphate carboxylase of oryza sativa l. and pennisetum americanum (l.) leeke. | 1985 | 4030003 | |
| selection and nuclear dna analysis of cell hybrids between daucus carota and oryza sativa. | in order to study the fate of the parental genomes in somatic cell hybrids between distantly related species, protoplasts from cultured cells of daucus carota and oryza sativa were fused. selective conditions resulted, exclusively, in the growth of hybrid colonies which combined the capacity to multiply of carrot cells with the natural resistance to a2ca of rice cells. a methodology for measuring the relative contribution of the parental cells to the hybrid nuclear genome has been worked out. th ... | 1985 | 23196174 |
| structural relationship among the rice glutelin polypeptides. | when the glutelin protein fraction of rice (oryza sativa l.) seeds was fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, three size classes of proteins, 51 kilodaltons (kd), 34 to 37 kd, and 21 to 22 kd, as well as a contaminating prolamine polypeptide of 14 kd were detected. antibodies were raised against these proteins and employed in studies to determine whether a precursor-product relationship existed among the glutelin components. antibodies of the 34 to 37 kd and 2 ... | 1986 | 16664896 |
| the effect of submergence, ethylene and gibberellin on polyamines and their biosynthetic enzymes in deepwater-rice internodes. | submergence and treatment with ethylene or gibberellic acid (ga3) stimulates rapid growth in internodes of deepwater rice (oryza sativa l. cv. "habiganj aman ii"). this growth is based on greatly enhanced rate of cell-division activity in the intercalary meristem (im) and on increased cell elongation. we chose polyamine biosynthesis as a biochemical marker for cell-division activity in the im of rice stems. upon submergence of the plant, the activity of s-adenosylmethionine decarboxylase (samdc; ... | 1986 | 24232756 |
| anatomical analysis of growth and developmental patterns in the internode of deepwater rice. | submergence of the stem induces rapid internodal elongation in deepwater rice (oryza sativa l. cv. "habiganj aman ii"). a comparative anatomical study of internodes isolated from airgrown and partially submerged rice plants was undertaken to localize and characterize regions of growth and differentiation in rice stems. longitudinal sections were examined by light and scanning-electron microscopy. based on cell-size analysis, three zones of internodal development were recognized: a zone of cell d ... | 1986 | 24232755 |
| immunochemical studies on the role of the golgi complex in protein-body formation in rice seeds. | antibodies raised against purified glutelins and prolamines were employed as probes to study the cellular routes by which these proteins are deposited into protein bodies of rice (oryza sativa l.) endosperm. three morphologically distinct protein bodies, large spherical, small spherical, and irregularly-shaped, were observed, in agreement with existing reports. immunocytochemical studies showed the presence of glutelins in the irregularly-shaped protein bodies while the prolamines were found in ... | 1986 | 24232753 |
| isolation and hypoglycemic activity of oryzarans a, b, c, and d: glycans of oryza sativa roots. | 1986 | 3562666 | |
| extent of heterosis in rice (oryza sativa l.) under cold stress conditions - yield and its components. | the nature and extent of heterosis for grain yield and its component characters was estimated in a seven-parent f1-diallel cross in rice under the cold stress conditions of palampur, representing sub-temperate climate of the mid hills of himachal pradesh. the highest heterotic effects for grain yield were observed in 'himalaya 1'/'phul patas 72' and 'china 988'/'himdhan' hybrids, which gave 60.36 and 32.48% heterosis, respectively, over mid parent, 38.76 and 26.86% heterosis, respectively, over ... | 1986 | 24240759 |
| regeneration of plants from long-term cultures of oryza sativa l. | root and embryo derived callus tissues of rice grown on sucrose alone as carbon source lost their ability to organise shoots by 75 and 100 days in culture respectively. along with 2% sucrose, either 3% sorbitol or 3% mannitol was found to be necessary in the growth medium for the callus to regenerate whole plants over a period of 1400 days without any decline in the shoot forming ability. our results indicated that incorporation of sorbitol or mannitol in the callus proliferating medium provides ... | 1986 | 24248307 |
| heat inactivation of starch synthase in wheat endosperm tissue. | the effect of temperature on accumulation of starch was studied in grain slices of wheat (triticum aestivum cv sun9e), taken 15 days after anthesis. as compared with pretreatment of such slices at 25 degrees c, pretreatment at 30 or 35 degrees c reduced the subsequent conversion of sucrose to starch. in contrast to rice (oryza sativa cv calrose), pretreatment of wheat soluble starch synthase in vitro at 30 degrees c or higher temperatures reduced its activity. in zymograms using nondenaturing po ... | 1986 | 16664836 |
| anther culture in rice: iv. the effect of abscisic acid on plant regeneration. | the effect of abscisic acid (aba) on plant regeneration in anther-derived calli of rice oryza sativa l. varieties taipei 177, taipei 309 and fujisaka 5 was studied.aba at concentrations up to 4×10(-6) m stimulated fresh weight increase in taipei 309 and fujisaka 5 while higher concentrations effected corresponding weight decreases in the three varieties tested. relatively high aba concentrations resulted in decreased callus size and production of more compact and whitish calli. aba increased the ... | 1986 | 24248053 |
| cytochemical studies of callus development from microspore in cultured anther of rice. | cytochemical studies of androgenic anthers of oryza sativa picked from the culture at 2 day intervals from 0 to 40 days have been carried out. glutaradehyde-oso4-fixed and plastic-embedded sections were stained with tbo, sbb and pas for acidic polymers, lipids and polysaccharides respectively. among the population only 4% of microspores, which accumulate abundant amorphous lipid in the first few days of culture, are androgenic. less than 30%, which have many lipid granules and some amorphous lip ... | 1986 | 24248049 |
| plant regeneration and initiation of cell suspensions from root-tip derived callus of oryza sativa l. (rice). | root-tip derived suspended callus of oryza sativa cv. thaipei showed the capacity for plant regeneration via organogenesis. cell cultures were induced in liquid murashige-skoog medium containing 2 mg/l 2.4-dichlorophenoxyacetic acid. dicamba or picloram were effective for induction of organogenesis. shoots and roots differentiated following subculture on medium lacking auxins but containing kinetin. at 1 and 4 mg/l dicamba and 1 mg/l picloram normal green plants were regenerated whereas with 7 m ... | 1986 | 24248041 |
| plant regeneration from protoplast-derived callus of rice (oryza sativa l.). | protoplasts isolated from cultured rice cells of an a-58 cytoplasmic male sterile line (a-58 ms)(oryza sativa l.) were used to investigate the regeneration of rice plants. a cultured cell line (t3) of a-58 ms with a high growth rate and dense cytoplasm was selected. about 10% of the protoplasts prepared from this established cell line plated in ry-2 (a new medium) formed colonies. the calli formed shoots and roots in the regeneration medium and developed into whole plants.protoplasts also were p ... | 1986 | 24248040 |
| genotypic variability for callus formation and plant regeneration in rice (oryza sativa l.). | sixty rice varieties (oryza sativa l.), belonging to three subspecies, japonica, indica and javanica (some japonicaxindica hybrids were included), were compared for their capacity for callus growth and plant regeneration. tissue cultures initiated from mature seeds on murashige and skoog's (1962) medium with 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-d) were transferred to a medium containing 0.02 mg/l 2,4-d and 10 mg/l kinetin, from which plantlets were regenerated. large variabilities in callu ... | 1986 | 24247763 |