Publications

molecular profiles of human melanoma-associated antigens.	melanoma-associated antigens (maa) shed into spent culture medium of intrinsically radiolabeled melanoma cells react specifically with monoclonal and polyclonal anti-melanoma xenoantiserums and are represented by two glycoproteins with molecular weights of 240,000 (240k) and 94,000 (94k): 240k is present only on melanoma cells whereas 94k is also found on carcinoma cells and on fetal melanocytes. both 240k and 94k have been obtained radiochemically pure by utilizing cellulose ion-exchange and an ...	1981	7461145
galactose oxidase labeling of membrane proteins from human brain white matter.	the use of galactose oxidase (ec 1.1.3.9) and tritiated sodium borohydride for labeling of membrane glycoproteins, described by gahmberg and hakomori, has previously been applied to the study of myelin glycoproteins of experimental animals. rat brain myelin glycoproteins have been studied by sequential lectin affinity of chromatography and recently the lectin-binding capacity of rat central nervous system myelin glycoproteins has been characterized. complex heterogeneity of the glycoprotein patt ...	1981	7470863
the amino-acid sequence of the alpha subunit of the mitogenic lectin from vicia sativa.	the complete amino acid sequence of the alpha chain of the mitogenic lectin from vicia sativa has been determined. the polypeptide was digested by trypsin and chymotrypsin. the fragments were isolated and most of them were sequenced by automatic solid-phase edman degradation. a total sequence of 52 amino acid residues was obtained which is homologous to the alpha subunits of the lectins from pisum sativum, lens culinaris and vicia faba. the central part (residues 13-32) in particular is complete ...	1981	7202414
functional and biochemical characteristics of human "null" lymphoid cells.	human peripheral null cells (non-t and non-b lymphocytes) were isolated by removing b lymphocytes and monocytes on nylon-wool columns and by e rosette-forming cells depletion. the null cell population contained less than 1% of sig-bearing cells, cells with complement receptors (eac+), and e mouse rosette-forming cells, but was contaminated by about 5% of t lymphocytes and 10% of monocytes. high affinity fc receptors were present on 12% of the null cells. no intracytoplasmic immunoglobulins (cigs ...	1981	7222212
the structure of the lentil (lens culinaris) lectin. amino acid sequence determination and prediction of the secondary structure.	the subunit structure and complete amino acid sequence of the lectin extracted from lens culinaris (lcl) seeds was determined. in previous studies, the primary structure of the alpha-chain (mr = 5,710) was shown to be homologous to the alpha-chain of the lectin from pisum sativum, the vicia cracca glucose-specific lectin, and a region in the middle of the concanavalin a sequence (residues 70-121). the complete amino acid sequence of the beta-chain (mr = 17,572) has been determined from 11 trypti ...	1981	7240155
the carbohydrate-binding specificity of pea and lentil lectins. fucose is an important determinant.	the carbohydrate-binding specificities of pea lectin and lentil lectin have been determined by testing the ability of radioactively labeled glycopeptides to bind to columns of pea lectin-sepharose and lentil lectin-sepharose. the presence of a fucose residue attached to the asparagine-linked n-acetylglucosamine residue of the test glycopeptide was essential for high affinity binding to both pea and lentil lectin-sepharose but not to concanavalin a-sepharose. in addition to fucose, 2 alpha-mannos ...	1981	7240233
concanavalin-a binding to acetylcholine receptors: identification of two forms of receptor in denervated rat muscle.	the interaction of the nicotinic acetylcholine receptor from denervated rat muscle (achr, solubilized in triton x-100) and concanavalin-a (con-a) was studied by soluble con-a competing with the binding of achr to agarose immobilized con-a, by immuno-precipitation of con-a-achr complexes and by inhibition of alpha-bungarotoxin binding to achr. results showed that con-a bound to 85% of all achr molecules at multiple cooperative binding sites. con-a caused a partial inhibition of toxin binding to a ...	1981	7241383
specificity of twelve lectins towards oligosaccharides and glycopeptides related to n-glycosylproteins.	glycopeptides and oligosaccharides of either the n-acetyllactosaminic or the oligomannosidic type derived from glycoproteins containing the n-glycosylamine linkage were used to define the specificity of different lectins (concanavalin a, lens culinaris agglutinin, vicia faba agglutinin, pisum sativum agglutinin, ricinus communis agglutinins, soybean agglutinin, wheat germ agglutinin, solanum tuberosum agglutinin, datura stramonium agglutinin, lotus tetragonolobus agglutinin, ulex europeus agglut ...	1981	7262089
the carbohydrate moiety of band 3 glycoprotein of human erythrocyte membranes. structures of lower molecular weight oligosaccharides.	band 3 glycoprotein purified from human blood group a erythrocyte membranes was subjected to hydrazinolysis to release carbohydrate moieties. the neutral oligosaccharide fraction, which constituted more than 85% of the total carbohydrates released, was fractionated by gel chromatography on a column of sephadex g-50 into two fractions, i.e. higher molecular weight and lower molecular weight fractions. from the lower molecular weight fraction, three oligosaccharides were obtained by chromatographi ...	1981	7287720
purification of measles virus glycoproteins and their integration into artificial lipid membranes.	we report a simple method for the isolation of the measles virus glycoproteins, and their subsequent incorporation into artificial lipid bilayers. the two viral glycoproteins, ha and f, were isolated in preparative amounts from disrupted purified virus by lentil lectin affinity chromatography. the proteins were reconstituted into single bilayer lipid vesicles by: (i) exchanging the non-dialysable detergent nonidet p40 (np40) for a dialysable one, octylglucoside, while the proteins were immobiliz ...	1981	7288404
purification of measles virus h polypeptide and of f polypeptide.	measles virus was disrupted by tween 80 and ether and subjected to isoelectric focusing in granular gel. the two surface envelope polypeptides, the one showing haemagglutinating activity (h) and the one making up the structural basis of the haemolytic and fusion activity (f) banded together at ph 5.2. the two envelope polypeptides were also isolated together after adsorption to a lentil-lectin column. separation of the two polypeptides was performed by gel filtration on sephadex g-150 in the pre ...	1981	7295039
purification and characterization of measles virus haemagglutinin protein g.	the 79 000 mol. wt. measles virion membrane glycoprotein g has been isolated from purified measles virus. ultracentrifugation of 2% triton x-100-treated measles virus produced a soluble supernatant fraction containing both g and f, the other external viral membrane protein. lentil lectin-sepharose and sephacryl s-300 column chromatography of this fraction gave a pure preparation of g protein. sucrose density-gradient centrifugation and sds-polyacrylamide gel electrophoresis revealed that g was i ...	1981	7299372
lectin cytochemistry of carbohydrates on cell membranes of rat cerebellum.	wheat germ agglutinin (wga), ricinis communis agglutinin (rca) and lens culinaris (lc) lectins have been used to characterize carbohydrates of neuronal membrane systems in rat and chick cerebellum. wga and rca both label golgi membrane cisternae on the side of the membrane facing the cisternal space but they do not label other elements of the granular and agranular endoplasmic reticulum (er). wga labels the plasma membrane generally and wga binding sites are concentrated within the synaptic clef ...	1981	7310443
characterization of surface antigens of trichinella spiralis infective larvae.	infective larvae of trichinella spiralis were surface-labelled with radioactive iodine, and the products were characterized biochemically and immunochemically. the labelled material was restricted to two basic subunits: a lentil lectin-adherent glycoprotein (gp), mol. wt 47k, and a lentil lectin-nonadherent protein fraction (p), mol. wt 55k. both of these form homologous dimers through as yet unspecified covalent bonds to yield gp90 and p105. gp is further polymerized into higher molecular weigh ...	1981	7322614
regulatory role of ige-binding factors from rat t lymphocytes. v. formation of ige-potentiating factor by t lymphocytes from rats treated with bordetella pertussis vaccine.	an i.p. injection of bordetella pertussis vaccine (bp) into rats induced the formation of soluble factors that had affinity for ige (ige-binding factors). the factor was detected in the serum of bp-treated animals 5 to 7 days after the treatment. their circulating lymphocytes as well as spleen cells spontaneously released ige-binding factors in the serum of bp-treated rats and those released from their circulating lymphocytes had affinity for lentil lectin, and the ability to selectively potenti ...	1981	6970222
lymphocytes bearing fc receptors for ige. v. effect of tunicamycin on the formation of ige-potentiating factor and ige-suppressive factor by con a-activated lymphocytes.	attempts were made to induce the formation of soluble factors having affinity for ige (ige-binding factors) by activated t cells. normal rat mesenteric lymph node (mln) cells were cultured in 1 microgram/ml con a for 48 hr or in 10 microgram/ml con a for 72 hr, and con a-activated lymphocytes were incubated with rat ige. it was found that ige induced an increase in the proportion of fc epsilon r(+) cells in the con a-activated cells and formation of ige-binding factors. however, the nature of ig ...	1981	6970224
lymphocytes bearing fc receptors for ige. vi. suppressive effect of glucocorticoids on the expression of fc epsilon receptors and glycosylation of ige-binding factors.	incubation of rat lymphocytes with homologous ige induced an increase in fc epsilon r(+) lymphocytes and the formation of ige-binding factors. pretreatment of rat lymphocytes with 1 to 5 microm dexamethasone, however, prevented the ige-induced expression of fc epsilon r on both b and t lymphocytes. upon incubation with ige, t cells activated with 10 micrograms/ml con a produced ige-potentiating factors that had affinity for lentil lectin and selectively enhanced the ige response. pretreatment of ...	1981	6972964
analysis of human t-cell antigens by one- and two-dimensional polyacrylamide gel electrophoresis.	one- and two-dimensional polyacrylamide gel electrophoresis (page) was performed on immunoprecipitates formed between anti-human t-cell xeno-antiserum (ats) and cell-surface glycoproteins of human lymphocytes, that had been radioiodinated by lactoperoxidase and purified on a lentil lectin-coupled sepharose 4b column. in some experiments, the cells were 3h-labeled by periodate-tritiated borohydride. ats that was absorbed with b cells recognized a number of cell-surface antigens expressed preferen ...	1981	6974300
skin allograft survival in lentil lectin-treated mice and rats.		1981	7022800
characterization of a plasma membrane-associated plasminogen activator on thymocytes.	plasma membranes isolated from normal thymocytes of hamster and rats were found to exhibit neutral protease activity toward 125i-labeled casein. the plasma membrane-associated proteases were completely inhibited by the serine protease inhibitors, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride and p-nitrophenyl-p-guanidinobenzoate, partially inhibited by soybean trypsin inhibitor and antipain, but were only weakly inhibited by l-1-tosylamino-2-phenylethyl chloromethyl ketone. the plas ...	1981	6793069
further characterization of the insulin receptor glycosidic moiety in rat adipocytes.	simultaneous or sequential treatment of rat adipocytes with neuraminidase plus beta-galactosidase decreased insulin binding by 43%. no modification was observed with either enzyme individually. alpha-mannosidase enhanced insulin binding (38%), whereas beta-n-acetylglucosaminidase and alpha-l-fucosidase were ineffective. lectins that interact with galactose (ricinus communis i, rcai), mannose, lens culinaris agglutinin (lca), concanavalin a (con a) or n-acetylglucosamine (wheat-germ agglutinin, w ...	1981	6793421
developmental stage-specific changes in lectin binding to mouse cerebellar cells in vitro.	eleven fluorescein isothiocyanate-conjugated (fitc) lectins, each with distinct carbohydrate-binding properties, were used to assess cell surface glycoconjugates of embryonic and early postnatal cerebellar cells in vitro. fluorescence staining of embryonic day 13 (e13) cerebellar cells with fitc ricinus communis agglutinin diminished markedly between 24 and 72 hr in vitro. no staining of postnatal day 0 (p0) or postnatal day 7 (p7) cells was observed with fitc ricinus communis agglutinin. a simi ...	1981	6793697
isolation, purification, and partial characterization of platelet membrane glycoproteins iib and iiia.	platelet membrane glycoproteins iib and iiia were isolated and purified from human platelet membranes using lentil lectin affinity chromatography and electrophoretic elution from sodium dodecyl sulfate-polyacrylamide gels. two-dimensional immunoelectrophoresis of a mixture of the purified proteins against monospecific antisera showed antigenic uniqueness of the separate polypeptides. computerized analysis of autoradiographs of two-dimensional tryptic 125i peptide maps revealed that the two glyco ...	1981	6450761
regulation of ige response by ige binding factors.	t lymphocytes from rats infected with nippostrongylus brasiliensis (nb) release a soluble factor with affinity for ige that selectively potentiates the ige response to an unrelated antigen. the factor is derived from fc omega receptors on t cells, binds to surface ige on precursors of ige-forming cells, and enhances their differentiation. the factor had a molecular weight between 10,000 to 20,000 and an affinity for lentil lectin. t cells from nb-infected rats formed another ige-binding factor u ...	1981	6453729
induction of human antigen-specific suppressor factors in vitro.	based on methods used for the in vitro induction of antigen-specific suppressor cells in the mouse, we have cultured ficoll-isopaque-separated human blood cells with high dose of antigen (100 microgram/ml) in marbrook culture vessels for 4 days. the resulting cells, when further recultured for 24 hr with a low dose of antigen (1 microgram/ml), released into the supernatant material, termed 'suppressor factor', which inhibited, in an antigen-specific manner, the antibody response of mouse spleen ...	1981	6169475
the qa-1 alloantigens. i. identification and molecular weight characterization of glycoproteins controlled by the qa-1a and qa-1b alleles.	splenocytes from the qa-tla congenic strain pairs, a and a-tlab or b6 and b6-tlaa, were biosynthetically labeled with 3h-amino acids or cell surface labeled with 125i. membrane proteins were solubilized with detergent and chromatographed on lentil lectin-sepharose, and the resulting adherent pools were immunoprecipitated with antisera specific for determinants controlled by the qa-1a and qa-1b alleles, qa-1.1 and qa-1.2, respectively. polyacrylamide gel electrophoresis analysis of immunoprecipit ...	1981	6170678
the biochemical characterization of a cell surface antigen associated with acute lymphoblastic leukemia and lymphocyte precursors.	the acute lymphoblastic leukemia- (all) associated membrane antigen is a single glycosylated polypeptide of approximate m.w. of 100,000 (gp100), containing no intrachain disulfide linkages. approximately 50% of gp100 will bind to lentil lectin, whereas 100% will bind to the lectin from ricinus communis. both lentil-binding and lentil nonbinding forms of the antigen appear to be identical by 2-dimensional isoelectric focusing/sds polyacrylamide gel electrophoresis and peptide mapping. carbohydrat ...	1981	6163827
separation and comparison of human tl-like antigens and hla(a, b, c) antigens expressed on cultured t cells.	beta 2-microglobulin-bound t-cell membrane components containing both human tl-like antigens and hla(a, b, c) antigens were partially purified from renex 30-solubilized membrane material of cells of a human t-cell-type leukemia cell line, hpb-all. the radioiodinated preparation was subjected to limited papain digestion; the hla(a, b, c) antigens split, whereas a large portion of the human tl-like antigens remained intact. the antigen molecules were recovered by lentil-lectin affinity chromatogra ...	1981	6164635
antigen-specific t lymphocyte clones. ii. purification and biological characterization of an antigen-specific suppressive protein synthesized by cloned t cells.	we have generated an antigen-specific t suppressor clone that synthesizes 70,000-mol wt peptides that have antigen-specific-binding activity. although these data also indicated that antigen-binding peptides completely inhibited the in vitro primary response to a complex antigen, suppression might reflect the combined biologic activities of many different 70-mol wt polypeptides or polypeptides associated with the 70,000-mol wt material by noncovalent interactions. the protein responsible for anti ...	1981	6166714
biochemical characterization including amino acid and carbohydrate compositions of canine and human brain thy-1 antigen.	human and canine brain thy-1 antigens were solubilized in deoxycholate and antigen activity was followed both by conventional absorbed anti-brain xenosera of proven specificity and by mouse monoclonal antibodies to canine and human thy-1. it is shown that greater than 80% of thy-1 activity in the dog and man binds to lentil lectin, that the mobility on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of canine and human thy-1 is identical with that of rat thy-1 and that the stokes radi ...	1981	6119980
production and characterization of monoclonal antibody to a melanoma specific glycoprotein.	an immunogen consisting of a 4m urea extract derived from human melanoma cells (m14), that was devoid of hla-a,b,c, hla-dr antigens and fibronectin was adsorbed to lens culinaris lectin-sepharose 4b and used to immunize mice for production of monoclonal antibody to a melanoma-specific glycoprotein. screening for hybridomas secreting antibodies to melanoma associated antigens was facilitated by use of a solid phase target antigen of chemically defined medium of melanoma cells (cdm). use of these ...	1981	6208119
fractionation and characterization of allergens extracted from eggs of schistosoma japonicum.	allergenic components were partially purified from the crude extract of eggs of schistosoma japonicum by gel filtration on sephadex g-200 and ion-exchange chromatography on deae-sephadex a-50. allergenic activity was assayed by the prausnitz-küstner type skin test with infected mouse sera and radioallergosorbent test with human ser. s. japonicum egg allergens (jeal) showed single and three allergenic peaks, by isoelectrofocusing (pi = 4.8) and polyacrylamide gel electrophoresis, respectively. th ...	1981	6257614
characterization of human platelet surface and intracellular membranes isolated by free flow electrophoresis.	high voltage free flow electrophoresis has been applied to the separation of human platelet membranes. after short treatment with neuraminidase at the whole cell level, three membrane vesicle subpopulations have been isolated. using a surface label (125i-labeled lens culinaris lectin), the marker enzyme nadh-cytochrome c reductase, and lipid analysis, two of the fractions have been identified as of surface origin and the other consists of intracellular membrane elements. the distribution of aden ...	1981	6260785
glycoprotein characteristics of the sodium channel saxitoxin-binding component from mammalian sarcolemma.	the saxitoxin-binding component of the excitable membrane sodium channel exhibits glycoprotein characteristics as evidenced by its specific interaction with various agarose-immobilized lectins. the detergent-solubilized saxitoxin-binding component interacts quantitatively with immobilized wheat germ agglutinin and concanavalin a and fractionally with immobilized lens culinaris hemagglutinin and ricinus communis agglutinin. these lectins preferentially bind n-acetylglucosamine and sialic acid (wh ...	1981	6268157
extracellular folate deaminase of dictyostelium discoideum.	folate deaminase released from cells of dictyostelium discoideum is heterogeneous with respect to molecular weight and stability at 60 degrees c. the most heat-stable component isoelectrofocuses in a broad band at approx. ph 6. the km value of this component for folate is approx. 7 x 10(-7)m and mr approx. 40 000. the major portion if not all of the deaminase binds to immobilized concanavalin a and lentil lectin. extracellular folate deaminase has a ph-optimum of approx. ph 6.0. this is higher t ...	1981	6271253
alkaline phosphodiesterase i and alkaline phosphatase i in plasma membranes of herpes simplex virus type 1 transformed hamster cells.	plasma membrane extracts from herpes simplex virus type 1 transformed hamster embryo fibroblasts were chromatographed on lens culinaris lectin coupled to sepharose (lch-sepharose) and analysed by dodecyl sulphate polyacrylamide gel electrophoresis. coomassie blue-staining revealed two major protein bands with apparent molecular weights of 125 000 and of about 75 000-90 000. in plasma membranes isolated from these tumor cells prior labeled with [3h]fucose or [3h]glucosamine these bands contained ...	1982	6277377
lectin affinity chromatography of sindbis and rous sarcoma virus glycopeptides and oligosaccharides.	glycopeptides and endogly cosidase-digested oligosaccharides from [3h]mannose-labeled rous sarcoma virus and sinbis virus have been fractionated by lentil lectin-sepharose and concanavalin a-agarose affinity chromatography and subsequently analyzed by biogel p-4 gel filtration. only a specific subset of the con a-bound asparaginly-oligosaccharides from he two viruses was also bound to lentil lectin, and this freaction apparently represented fucose-containing, diantennary acidic-type structures ( ...	1982	6286708
vil-a1, a monoclonal antibody reactive with common acute lymphatic leukemia cells.	the vil-a1 monoclonal antibody raised against reh cells reacts with common acute lymphatic leukemia (call) cells but not with normal or malignant b or t lymphocytes. it also shows no binding to normal or malignant myeloid, monocytic or erythroid cells, nor does it react with thrombocytes. the antibody is of igm class and lyses call cells very efficiently in the presence of rabbit but not human complement. immunoprecipitation experiments followed by sds-polyacrylamide gel electrophoresis under re ...	1982	6193374
carbohydrate complexity of the mouse thymocyte thy-1 glycoprotein as demonstrated by lectin affinity and isoelectric focusing.	the thy-1 glycoprotein of mouse thymocytes was analysed with regard to the properties of its carbohydrate part. of the different lectins tested, partial binding of thy-1 from membrane extracts was found to lens culinaris agglutinin (approximately 50%) and wheat germ agglutinin (approximately 25%). in contrast, concanavalin a bound all thy-1. the results indicate that there is considerable microheterogeneity in the oligosaccaride chains within the thy-1 population. thy-1 was purified from thymocy ...	1982	6121705
purification and chemical characterisation of membrane glycoproteins from rat thymocytes and brain, recognised by monoclonal antibody mrc ox 2.	the mrc ox 2 monoclonal antibody recognises antigens present on rat thymocytes, brain, follicular dendritic cells in lymphoid organs, vascular endothelium, some smooth muscle and b-lymphocytes. the ox 2 antigens recognised by this antibody were purified from brain and thymus, by solubilisation with sodium deoxycholate, affinity chromatography with mrc ox 2 antibody and gel filtration. the purified brain and thymocyte ox 2 antigens were glycoproteins with apparent mr 41000 and 47000 respectively ...	1982	6129975
horseradish peroxidase (hrp) conjugates of cholera toxin and lectins are more sensitive retrogradely transported markers than free hrp.	horseradish peroxidase (hrp) conjugates of 8 different lectins (wheat germ agglutinin, ricinus communis i and ii, peanut agglutinin, lens culinaris, soybean agglutinin, limulus polyhemus, ulex europaeus i) and cholera toxin (ct) or free hrp (fhrp) were individually injected into the submandibular gland (smg) or anterior chamber (ac) of the eye and the retrogradely labeled neurons in the superior cervical ganglion (scg) were quantitated. the effect of using 3 different cross-linking reagents (glu ...	1982	6173093
identification of an oncofetal antigen (gp90) on murine b16 melanoma cells.	a xenoantiserum to murine b16 melanoma cells was developed by immunizing rabbits with cultured b16 melanoma cells. this antiserum could, after extensive absorption with normal c57bl/6 mouse tissues, react with syngeneic (b16) and allogeneic (hp) melanomas as well as with other murine neoplasms, including syngeneic 75s adenocarcinoma, allogeneic myeloma and leukemic t cell lines. the antiserum also cross-reacted with syngeneic fetal fibroblasts and with an allogeneic fetal fibroblast cell line (s ...	1982	6889531
electroimmunochemical analysis of plasma membrane vesicles from saccharomyces cerevisiae.	plasma membrane vesicles of saccharomyces cerevisiae were extracted with 1% (w/v) triton x-100 and the solubilized proteins examined by crossed immunoelectrophoresis using rabbit antibodies against the vesicles. solubilization was shown to be nonselective and 23 immunoprecipitates were observed reproducibly. four glycoproteins were identified by interaction with concanavalin a and lentil lectin, either immobilized on agarose beads in an intermediate gel or incorporated in the free form in the fi ...	1982	6749264
the pathogenic antigen of heymann nephritis is a membrane glycoprotein of the renal proximal tubule brush border.	purified brush border fractions prepared from rat kidneys were solubilized in detergent, iodinated, and subjected to immunoprecipitation to identify the pathogenic antigen present in brush border membranes that is responsible for the production of heymann nephritis (hn). purified igg prepared from the sera of rabbits or rats immunized with a crude cortical preparation, known as fx1a, precipitated multiple peptides, whereas igg eluted from glomeruli of rats with active or passive hn specifically ...	1982	6752952
carbohydrate determinants involved in both the binding and action of insulin in rat adipocytes.	the insulin receptor apparent affinity was markedly decreased in fat cells treated with lectins specific either for d-galactose (ricinus communis agglutinin i, rcai), d-mannose (concanavalin a, con a, lens culinaris agglutinin, lca) or n-acetyl-d-glucosamine (wheat germ agglutinin, wga), as indicated by a rightward shift of the binding competition curves and almost lineared scatchard plots. limulus polyphemus agglutinin (lpa), specific for sialic acid, was ineffective. all lectins enhanced 2-deo ...	1982	6759201
properties of a surface antigen expressed on activated human thymus-derived lymphocytes.	we have identified previously a quantitatively minor membrane protein (p28) with an apparent reduced m.w. of 28,000, which is biosynthetically labeled in activated human lymphocytes. rabbit antisera with activity directed against p28 (alpha-atc) were prepared and p28 was identified by immunoprecipitation in np-40 extracts of activated, extrinsically labeled lymphocytes. p28 was not expressed in appreciable amounts by unstimulated t cells, stimulated or unstimulated b cells, null cells, or adhere ...	1982	6801123
multiple molecular forms of rat brain enkephalinase.	rat brain enkephalinase has been partially purified by ion exchange chromatography, chromatofocusing, and affinity chromatography on immobilized lectins. ion exchange chromatography resolved two principle forms of enkephalinase designated a1 and a2. both enkephalinase a1 and a2 are bound to immobilized lentil lectin while chromatography on immobilized wheat germ lectin resolved each of the principle forms into two subforms, a1, 1, a1, 2, a2, 1, and a2, 2. all four enkephalinase forms have simila ...	1982	6813615
lectins as inducers of interferon-gamma production in human lymphocytes: lentil lectin is highly efficient.	several of many tested plant lectins induced interferon (ifn) production in cultures of human peripheral blood mononuclear leucocytes (pbl). the mannose-binding lectin obtained from lens culinaris (lcl) was a particularly efficient inducer of trypsin-sensitive antiviral activity, which qualified as ifn-gamma because it was 90-95% destroyed by ph 2 treatment but not neutralized by anti-ifn-gamma antibodies. however, such antibodies neutralized the residual 5-10% ph 2-resistant ifn, which therefor ...	1982	6815785
nonspecific and cooperative binding of lectins to microorganisms.	binding of lectins to microbial cell walls was investigated by fluorimetric titration and scatchard plot. data were correlated with agglutinability. concanavalin a and lectins of wheat germ, soybean, pea, lentil, and peanut were tested against escherichia coli. micrococcus luteus, lactobacillus plantarum, and bacillus subtilis. in cases where binding occurred, it was either nonspecific or positively cooperative. agglutination was observed only in those combinations of lectin and microorganism th ...	1982	6817352
flow cytometric analysis of lectin binding to human peripheral blood lymphocytes.	the binding of fluorescein-conjugated lentil lectin and concanavalin a to the surface membrane of human peripheral blood lymphocytes was studied by flow cytometry. the lymphocytes bound 3-fold more lentil lectin molecules compared with concanavalin a molecules and lentil lectin binding approached saturation at a much lower concentration than did that of concanavalin a. lentil lectin identified two groups of lymphocytes: a low-binding t-cell fraction and a high-binding b-cell-enriched fraction. c ...	1982	6818949
identification and purification of a mr 75,000 cell surface human melanoma-associated antigen.	surface macromolecules on cultured human melanoma cells wee radioiodinated by a lactoperoxidase method. macromolecules shed into culture medium were collected, concentrated, and fractionated on sepharose 6b and lentil lectin-sepharose. radioactivity associated with macromolecules was assayed by precipitation with trichloracetic acid, and that associated with melanoma-associated antigens (maa) was assayed by specific immunoprecipitation with xenogeneic anti-melanoma serum. following the last step ...	1982	7042082
synergistic effect of lentil lectin and antithymocyte serum on rat skin allograft survival.		1982	7046168
a cautionary note on the use of lentil lectin affinity chromatography in the presence of sodium deoxycholate.	when affinity chromatography with lentil lectin (lch)-sepharose was carried out in 0.5% (w/v) sodium deoxycholate (doc), 10 mm tris, ph 8.2, lch consistently appeared in the 0.1 m alpha-methyl-mannoside (alpha-meman)-eluted fraction. eluted lch was recovered in both the control and specific immunoprecipitates with antisera directed against components of the glycoprotein fraction, suggesting that at least a portion of the lch retained the capacity to interact with protein-bound carbohydrate. we s ...	1982	7057043
purification and characterization of a mannose/glucose-specific lectin from vicia cracca.	the seeds of vicia cracca are known to contain two lectins: an n-acetylgalactosamine-binding lectin, which reacts specifically with human erythrocytes of blood group a, and a mannose/glucose-binding lectin unspecific towards human blood groups. the second lectin is the object of the present work. purification was achieved by affinity chromatography on sephadex g-100. the native lectin has a molecular weight of 44,000. it is composed of two small (mr 5,700) and two large subunits (mr 17,500). bin ...	1982	7060560
red cell membrane protein anomalies in congenital dyserythropoietic anaemia, type ii (hemp as).	in all of six cases of congenital dyserythropoietic anaemia, type ii (hempas), gel electrophoresis in the presence of sds revealed abnormally rapid migration of the preponderant integral membrane protein, band 3. after proteolysis of intact cells, the remaining part of the band 3, comprising the intramembrane segment and the cytoplasmic domain, migrated electrophoretically as a single band, identical in mobility to that from normal cells treated in the same manner. the anomaly thus resides in th ...	1982	7066206
biosynthesis of gp160, the major trypsin-sensitive surface glycoprotein of macrophages.	the biosynthetic origin was investigated of gp160, the trypsin- and plasmin-sensitive surface glycoprotein of caseinate-elicited macrophages from guinea pigs. biosynthesized surface components were analyzed by reacting [35s]methionine-labeled macrophages with trinitrobenzene sulfonic acid and purifying trinitrophenyl-substituted surface proteins by immunoprecipitation. identification of gp160 was based on its molecular weight and unique trypsin sensitivity. in addition, [35s]methionine-labeled s ...	1982	7076683
lectin-reactive components in white matter membranes from normal and multiple sclerosis brains.	polypeptides derived from human white matter membranes reacted with the radioiodinated lectins concanavalin a, lens culinaris phytohemagglutinin, ricinus communis agglutinin and wheat germ agglutinin after electrophoresis in polyacrylamide pore gradient gels. the molecular weights of these lectin-reactive bands were estimated by comparison with radioiodinated protein standards by using the linear relationship between log of the molecular weight and log of the gel concentration reached by the pro ...	1982	7077325
isolation and characterization of plasma-membrane glycoproteins from pig epidermis.	1. non-desmosomal plasma membranes enriched in plasma-membrane marker enzymes and in metabolically labelled glycoproteins were isolated on a large scale from up to 500g of pig ear skin slices. sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and periodic acid/schiff staining revealed the presence of four major glycosylated components in the apparent molecular-weight range 150000-80000. 2. a large proportion of the marker enzymes, the d-[(3)h]glucosamine-labelled glycoproteins and the p ...	1982	7082290
intercellular adhesion of rat hepatocytes. identification of a cell surface glycoprotein involved in the initial adhesion process.	a cell surface molecule involved in intercellular adhesion between rat hepatocytes in vitro has been identified. papain-solubilized plasma membrane components were fractionated; the ability of the solubilized components to neutralize the inhibition of cell aggregation, caused by multispecific antibodies directed against the cell surface, was used to follow the purification. the purified components were used to immunize rabbits. the resulting antibodies effectively inhibited hepatocyte aggregatio ...	1982	7085603
lectin-binding characteristics of human natural killer cells.	human natural killer (nk) cells separated initially by density centrifugation of lymphocytes (e+) forming rosettes with sheep red blood cells (srbc), were further fractionated on gradients of bovine serum albumin (bsa). low density fractions contained effector cells which displayed high cytotoxicity against the nk-sensitive erythroleukaemic cell line, k562. these low density cells, which expressed receptors for fc and the monoclonal antibody okmi, showed enhanced cytotoxicity when treated with l ...	1982	7095832
purification of a native membrane-associated adenovirus tumor antigen.	a 15,000-dalton protein was purified from hela cells infected with adenovirus type 2. proteins solubilized from a membrane fraction of lytically infected cells was used as the starting material for purification. subsequent purification steps involved lentil-lectin, phosphocellulose, hydroxyapatite, deae-cellulose, and aminohexyl-sepharose chromatographies. a monospecific antiserum, raised against the purified protein, immunoprecipitated a 15,000-dalton protein encoded in early-region e1b (e1b/15 ...	1982	7097863
fractionation and identification of pig lymph node plasma membrane glycoproteins.	plasma membrane vesicles purified from pig mesenteric lymph node tissue were solubilized in 1% (w/v) sodium deoxycholate and the fractionation of both membrane proteins and glycoproteins assessed by gel filtration on aca 34. milligram quantities of the glycoprotein fraction, consisting of 12 distinct bands on sds-page ranging in apparent mol. wt from 12,000 to 250,000, were purified by affinity chromatography on lentil lectin-sepharose. identified among these by specific immunoprecipitation were ...	1982	7099163
human neutrophils endocytose multivalent ligands from the surface of schistosomula of schistosoma mansoni before membrane fusion.	human buffy coat cells adhering to schistosomula of schistosoma mansoni that were preincubated in fluorochrome-conjugated concanavalin a (con a), wheat germ agglutinin, lentil lectin, or purified igg from a hyperimmunized rabbit, were examined by fluorescence and transmission electron microscopy and by freeze-fracture. all four fluorochrome-conjugated multivalent ligands were homogeneously distributed on the parasite surface after preincubation. within 1-3 h after the addition of cells, large ar ...	1982	7107703
comparative analysis of procedures used to isolate variant antigen from trypanosoma brucei rhodesiense.	comparisons made among various procedures leading to the isolation of variant antigen from trypanosoma brucei rhodesiense bloodstream trypomastigotes. as a means of parasite disruption, freeze-thawing solubilized 36% more variant antigen than did sonication. protease inhibitors were important additions to the suspension prior to cellular disruption. if trypanosomal extracts were frozen for at least 1 wk prior to chromatographic isolation of variant antigen, recovery of the antigen was reduced by ...	1982	7119984
non-covalent interactions result in aggregation of surface antigens of the parasitic nematode trichinella spiralis.	surface antigens of three stages of the nematode worm trichinella spiralis has been labelled with iodine and examined by sodium dodecyl sulphate (sds)/polyacrylamide-gel electrophoresis under reducing and non-reducing conditions. a variety of interactions were defined: the infective larva surface antigens formed a spectrum of aggregates from 50kda to greater than 1000kda from subunits of 47kda and 90kda; in the adult worms of 60kda complex arose fron interaction between two dissimilar molecules ...	1982	7126193
tryptic peptide map analysis of the major human blood platelet membrane glycoproteins separated by two-dimensional polyacrylamide gel electrophoresis.	washed platelets were surface-labelled by lactoperoxidase catalyzed iodination and either the platelets or membranes were solubilized in detergent and applied to a wheat germ agglutinin-sepharose column and a lens culinaris lectin sepharose column coupled sequentially. the glycoproteins eluted from the lectin columns were separated by two-dimensional gel electrophoresis. alternatively, labelled whole platelets or membranes were solubilized and then directly separated by two-dimensional polyacry ...	1982	7126562
use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa.	paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. these were either isolated or purified by affinity column chromatography (ulex europeus, triticum vulgare, glycine max, and arachis hypogatea lectins) or commercial preparations (lotus tetragonolobus, canavalia ensiformis, helix pomatia, phaseolus vulgaris, and lens culinaris lectins) and conjugated to peroxidase (except for concanavalin a). these labeled lect ...	1982	7130674
similarity of acetylcholine receptors of denervated, innervated and embryonic chicken muscles. 1. molecular species and their purification.	acetylcholine receptors were purified to homogeneity from chicken embryonic, adult innervated and denervated muscles, by bio-specific chromatographies using immobilised alpha-neurotoxin and lentil lectin. a minimum specific activity for the pure receptor was estimated to be 6000 nmol alpha-toxin binding sites/g protein. for analysis, the receptors were radio-iodinated or tritiated to high specific radioactivity with succinimidyl-[2,3-3h]propionate. all of the iodinated protein present in the pur ...	1982	7140738
circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols.	the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ...	1982	7150429
cell kinetics and radiosensitivity of human lymphocytes stimulated by phytohemagglutinin, wistaria floribunda or lentil lectin.	the cell kinetics and the radiosensitivities of human lymphocytes (four donors) exposed to 200 rads of x-rays and stimulated with phytohemagglutinin (pha), wistaria floribunda (wfa) or lens culinaris (lch-a) extracts have been compared after cultivation times from 42 up to 54 h. all these mitogens are considered activating primarily t lymphocytes. pha is a tetrameric molecule, whereas wfa as well as lch-a are dimeric structures having only two reactive sites. pha displays a higher mitogenic acti ...	1982	7182059
biochemical and immunologic heterogeneity of ia glycoproteins isolated from a chronic lymphocytic leukemia.	ia glycoproteins have been isolated from human chronic lymphocytic leukemic cells (cll) by lens culinaris chromatography and by filtration on aca-34 ultrogel. ia antigenic activity, measured by inhibition of the cellular radioimmunoassay, was separated by gel filtration into 2 fractions, peak i and peak ii. monoclonal antibodies, produced against peak ii glycoproteins, appear to recognize different antigenic determinants of ia molecules. monoclonal antibody 18a4 reacted with ia molecules of peak ...	1982	6976373
formation of rat ige-binding factors by rat-mouse t cell hybridomas.	mesenteric lymph node (mln) cells of rats infected with nippostrongylus brasiliensis (nb) were obtained 8 days after infection and were incubated overnight with rat ige for the formation of ige-binding factors. these cells were then fused with bw 5147 mouse t lymphoma cells. two hybrid clones (a4 and b6) obtained by the fusion formed ige-binding factors upon incubation with ige. it was found that 1-hr incubation with 3 micrograms/ml of rat ige was sufficient to induce hybridoma cells to form ige ...	1982	6979575
characterization of a 75,000 mol. wt glycoprotein synthesized by guinea-pig t-lymphocytes: a possible homologue of lyt-1 antigen.	a xenoantiserum raised in rabbits by immunization with strain 13 guinea-pig antigen-activated t-lymphocytes was previously found to recognize a non-immunoglobulin, 75,000 mol. wt glycoprotein synthesized by guinea-pig t-cells. this protein, p75, has been further characterized to determine its biochemical properties and its expression by various cell types. p75 was found to be a single-chain protein which could be bound by the lectin lens culinaris hemagglutinin. it has an apparent mol. wt slight ...	1982	6984489
expression of cell surface lectins on activated human lymphoid cells.	a cell surface lectin found on activated human lymphoid cells has been identified and characterized using membrane glycoprotein micelles as probes. these micelles, which are large, water-soluble aggregates, are composed of glycoproteins isolated from detergent-solubilized membranes of human b lymphoblastoid cell lines by lens culinaris hemagglutinin affinity chromatography. the micelles have an average apparent molecular weight of 4 x 10(6) estimated by gel filtration and range in diameter from ...	1982	6214410
detection, isolation and characterization of cell free hla a and b antigens from human amniotic fluid.	cell-free hla a and b antigens, paternal and maternal, have been detected in human amniotic fluids obtained from women at 16-18 weeks' gestation. fractionation of amniotic fluid samples on sephadex showed that hla a and b antigen activity was mainly in the 40,000 to 70,000 mol. wt. fractions. lentil lectin sepharose 4b affinity chromatography of 30,000 to 70,000 sephadex molecular weight fraction of amniotic fluids isolated proteins which gave 2 bands, m and n, on discontinuous polyacrylamide ge ...	1982	6959379
structural studies of purified pig lymph node plasma membrane: association of cytoskeletal compounds with the plasma membrane and the effect of detergent solubilization.	the reproducibility of preparation, stability at 4 degrees c, and detergent solubilization characteristics of plasma membrane vesicles purified from domestic pig mesenteric lymph node tissue have been examined. it was found that 2% (w/v) nonidet p-40 solubilized 50-60% and 2% (w/v) sodium deoxycholate solubilized 60-70% of the total membrane protein. as judged by 125i-labelled lentil lectin staining of the sodium dodecyl sulfate--polyacrylamide gel electrophoresis patterns, 2% (w/v) nonidet p-40 ...	1982	7199963
affinity chromatographic purification of lentil lectin using immobilized yeast cells.	a model system for evaluating macroporous supports containing immobilized whole cells in affinity chromatographic applications is described. whole cells were immobilized in a polyacrylamide network in a two-step polymerization process. the affinity system discussed consists of immobilized cells ofsaccharomyces cervisiae in the purification of lentil lectin fromlens culinaris.	1982	24234090
cell surface carbohydrates in crithidia deanei: influence of the endosymbiote.	the cell surface of the symbiote-containing and symbiote-free strains of crithidia deanei were characterized comparatively by using 22 highly purified lectins with specificities for n-acetyl glucosamine, n-acetyl galactosamine, galactose, mannose-like residues, fucose and sialic acid. the specificity of the cell surface carbohydrate in both strains were analyzed by agglutination and lectin-binding assays. c. deanei with or without endosymbiote was specifically agglutinated by lectins from tritic ...	1982	7067702
biochemical characterization of the tetrodotoxin binding protein from electrophorus electricus.	biochemical properties of a detergent-solubilized tetrodotoxin binding component from electrophorus electricus have been examined and compared with those found for the membrane-bound protein. the toxin binding component was solubilized with high efficiency by a variety of nonionic detergents and with lower efficiency by sodium cholate and deoxycholate. detergent-solubilized preparations bound tetrodotoxin and saxitoxin tightly and specifically, and this binding was observed to be rapidly and irr ...	1982	6295460
analysis of murine ia antigen glycosylation by lectin affinity chromatography. i-ak alpha chain subspecies and beta chains are each glycosylated differently.	murine ia antigens consist of two glycosylated polypeptide chains, the alpha chain and the beta chain. we have used lectin affinity chromatography to confirm previous work in our laboratory that three distinct, differentially glycosylated i-ak alpha chains (alpha 1, alpha 2, and alpha 3) exist and to compare the carbohydrate of the alpha chain with that of the beta chain. glycopeptides derived from pronase digestion of [3h]mannose-labeled i-ak alpha 1, alpha 2, alpha 3, and beta chains were sequ ...	1982	6956576
association of membrane and cytoplasmic proteins with the cytoskeleton in blood platelets.	the association of membrane and cytoplasmic proteins with the cytoskeleton of resting and activated platelets was studied. glycoproteins were identified by labeling with 125i-labeled lectins (concanavalin a, wheat germ agglutinin, and lens culinaris). polypeptides, which are embedded in the lipid bilayer, have been identified by their photolabeling with the lipid-soluble reagent 5-[125i]iodonaphthyl 1-azide (125ina). cytoplasmic proteins were identified by their photolabeling with the intracellu ...	1982	7200800
studies on the mechanism of lectin-dependent t cell-mediated cytolysis: use of lens culinaris hemagglutinin a to define the role of lectin.		1982	6980578
lectin affinity chromatography of glycopeptides and oligosaccharides from normal and lectin-resistant chinese-hamster ovary cells.	the [3h]mannose-labelled glycopeptides from two lectin-resistant lines of chinese-hamster ovary cells were fractionated by chromatography on lentil lectin-sepharose and concanavalin a-agarose columns and subsequently analysed by gel filtration in comparison with the glycopeptides of the parental cell line. essentially all of the [3h]mannose-labelled asparaginyl-oligosaccharides from the 'single-mutant' cells selected for resistance to phytohaemagglutinin and the 'double-mutant' cells selected fo ...	1982	6890813
[fractionation of lymphocytes using affinity chromatography with 9 lectins].	lymphocyte subclasses from normal peripheral blood have been fractionated by affinity chromatography with lectins. concanavalin a (con a), lens culinaris lectin (lc), pisum sativum lectin (ps), phaseolus vulgaris lectin (pha), dolichos biflours lectin (db), glicine max lectin (sba), ricinus communis lectin (rca ii), tetragonolobus purpureus lectin (tp) and triticum vulgaris lectin (wga), were coupled to sepharose 6mb, and lymphocytes labelled with 125i were eluted through the chromatographic col ...	1983	6675094
tumor-associated changes in plasma samples revealed by two-dimensional macromolecular mapping and selective lectin binding.	two-dimensional electrophoretic analysis of plasma samples from el-4 lymphoma-bearing c57 black mice revealed five 75 kd protein species in contrast with the presence of only two comparable components of similar migration in plasma from control animals. in contrast, no comparable alterations were observed in a comparison of plasma samples from l1210 tumor-bearing dba mice and the corresponding plasma from animals immune-suppressed with antilymphocytic serum or in control plasma from dba control ...	1983	6667450
purification and characterization of two proteolytic enzymes in the cotyledons of germinating lentils.	two proteases, one peptidehydrolase and one aminopeptidase, have been purified to homogeneity from cotyledons of germinating seeds of lens culinaris med. peptidehydrolase has an apparent molecular weight of 89,000 and an isoelectric point of 4.7. peptidehydrolase activity was not affected by metal chelators but it was affected by n-bromosuccinimide, phenylmethylsulfonyl fluoride and n-ethylmaleimide, suggesting the presence of tryptophan and serine residues together with free--sh groups in its a ...	1983	6361931
changes in nitrogen fractions and proteolytic activities in the cotyledons of germinating lentils.	changes in ninhydrin positive material, free amino acids and protein content during germination of seeds of lens culinaris med. have been studied. ninhydrin positive material and free amino acids reached their highest concentration at the fifth day of germination. total protein which represents 21% of the total dry weight of the lentil cotyledons, suffers a degradation of only 24% in seven days of germination; in the same period of time, reserve proteins underwent a degradation of 69%, legumin b ...	1983	6361930
cell-surface changes during mitogenic stimulation of lymphocytes assessed by the binding of wheat-germ agglutinin and other plant lectins.	lymphocytes from murine lymph node, cultured in the presence of an optimally mitogenic dose of phytohaemagglutinin, were stained with fluoresceinated lectins and analysed by flow cytometry. a marked increase in the ability of lymphocytes to bind wheat-germ agglutinin was observed that is particularly pronounced for the blast cells, reaching a maximum at about 40 h, when they are 5.5-times brighter than cells at zero time. the corresponding intensification of the small cells is 2-fold. much small ...	1983	6688188
t lymphocyte-mediated cytolysis. iii. delineation of mechanisms whereby mitogenic and non-mitogenic lectins mediate lymphocyte-target interaction.	we have investigated the mechanism(s) by which mitogenic (concanavalin a [con a], phytohaemagglutinin [pha] and lens culinaris agglutinin [lca]) and non-mitogenic (soybean agglutinin, peanut agglutinin, wheat-germ agglutinin and pokeweed mitogen) lectins mediate, non-specifically, lectin-dependent lymphocytotoxicity (ldcc). we show that non-mitogenic lectins are ineffective mediators of ldcc, due to their inability to mediate effective binding of effector cytotoxic t cells (ec) and target cells ...	1983	6603417
crystallization and preliminary x-ray diffraction studies of the mitogenic lentil (lens culinaris) lectin.		1983	6854638
characterization of a glycoprotein alpha-galactosidase from lentil seeds (lens culinaris).	alpha-galactosidase (alpha-d-galactoside galactohydrolase, ec 3.2.1.22), an enzyme responsible for mobilizing the raffinose family of oligosaccharides in legume seeds, has been isolated from lentils (lens culinaris) and purified about 4,000-fold. the sephadex gel filtration profile showed the presence of two forms of the enzyme, alpha-galactosidase i with an apparent mr = 160,000 and alpha-galactosidase ii of mr = 40,000. enzyme ii readily aggregates to form i when any attempt is made to concent ...	1983	6296089
comparison of amniotic fluid alpha-fetoprotein reactivity to lens culinaris agglutinin and concanavalin a in crossed-affinity immunoelectrophoresis: ancillary tests in the prenatal diagnosis of severe fetal malformations.	crossed-affinity immunoelectrophoresis of alpha-fetoprotein in amniotic fluid from 135 normal and 39 abnormal pregnancies (mainly neural-tube defects) was performed with a lectin, agglutinin from lens culinaris. results were compared with findings reported for another lectin, concanavalin a. three fractions of alpha-fetoprotein were obtained by reaction with lens culinaris agglutinin. the most weakly reactive fraction correlated strongly with the concanavalin a nonreactive fraction. with both le ...	1983	6184183
lectin binding patterns in diffuse large cell lymphoma.	the staining reaction of a panel of lectins in paraffin embedded lymph node specimens of diffuse large cell lymphoma was studied in relation to survival. in 47 of 49 patients, varying degrees of lectin binding were observed with ricinus communis agglutinin (rca), crude extract of arachis hypogaea (c-pna), concanavalin ensiformis a (con a), triticum vulgaris a (wga) and phaseolus vulgaris a (pha). binding was either absent or only minimal with pisum sativum a (psa) and lens culinaris a (lca). two ...	1983	6194878
histochemical studies on lectin binding in reactive lymphoid tissues.	using the avidin-biotin-labeled peroxidase complex (abc) method, the staining reaction of a panel of 12 biotin-labeled lectins was studied in formalin-fixed, paraffin-embedded reactive lymph nodes and tonsils. varying degrees of lectin binding were observed in lymphoid cells and macrophage-histiocytes with concanavalin ensiformis (con a), lens culinaris (lca), phaseolus vulgaris (pha), pisum sativum (psa), ricinus communis (rca), and triticum vulgaris (wga) agglutinins, but no evidence of bindin ...	1983	6827084
induction of polymorphonuclear leukocyte-mediated cytolysis by wheat germ agglutinin and antitumor antibody.	the role of polymorphonuclear leukocytes (pmns) as effector cells in tumor lysis was investigated in vitro. pmns were obtained from the peritoneal cavity of c3h/he mice injected ip with 2 ml of 12% casein sodium. these pmns could lyse murine mm46 tumor cells in the presence of the plant lectin, wheat germ agglutinin (lectin-dependent pmn-mediated cytolysis). pmns alone did not cause cytolysis. pmns obtained 6 hr after casein injection were effective against tumor target cells but those obtained ...	1983	6862146
competition among rhizobium leguminosarum strains for nodulation of lentils (lens esculenta).	thirty-one cultures of rhizobium leguminosarum were screened for effectiveness (c(2)h(2) reduction) on lentils (lens esculenta). fluorescent antibodies prepared against three of the most effective strains (hawaii 5-0, nitragin 92a3, and nitragin 128a12) exhibited a high degree of strain specificity; the antibodies reacted strongly with their homologous rhizobia in culture and with bacteroids in nodules. they did not cross-react with one another, and only weakly with 5 of the 47 other r. legumino ...	1983	16346257
molecular characterization of the human red cell rho(d) antigen.	human red cells of rh blood groups -d-/-d- ('super-d'), -/- (rhnull) and normal rho(d)+ cells were radioactively surface-labeled using the lactoperoxidase 125i method. polyacrylamide gel electrophoresis in the presence of sds followed by fluorography showed a strong enrichment of a polypeptide with an apparent mol. wt. of 28,0000-33,000 in the 125i-labeled -d-/-d- membranes. this polypeptide was specifically immune precipitated with anti-rho(d) antiserum. treatment of intact cells with trypsin o ...	1983	11894930
ige-binding factors from mouse t lymphocytes. i. formation of ige-binding factors by stimulation with homologous ige and interferon.	incubation of normal mouse spleen cells with homologous ige resulted in the formation of soluble factors that inhibited rosette formation of mouse fc epsilon r+ cells with ige-coated ox erythrocytes. the soluble factors could be absorbed with mouse or rat ige coupled to sepharose and recovered from the beads by acid elution. however, the factors had no affinity for either human ige or mouse igg. the ige-binding factors were derived from t cells. production of the factors required lyt1+ t cells a ...	1983	6217246
[possibilities of obtaining, by passage on plantlets, reproduction forms (porospores) in strains of alternaria (dematiaceous fungi) isolated from human cutaneous lesions and having lost the possibility of fructifying. preliminary results].	strains of alternaria (dematiaceous hyphomycetes) isolated from lesions in men or animals generally lose quickly their ability to fructify (porospores forming) after their isolation and keeping up on artificial culture mediums classicaly used in medical diagnostic laboratories. the authors, from the fact that alternaria are fungi which are normally parasites of living plants, put, on plantlets from germinating corn seeds (graminaceous plant) and lentil seeds (papilionaceous plant), two strains o ...	1983	6224543
modulation of the biologic activities of ige-binding factors. iii. switching of a t cell hybrid clone from the formation of ige-suppressive factor to the formation of ige-potentiating factor.	incubation of rat-mouse t cell hybridoma cells, 23b6, with rat immunoglobulin e (ige) results in the formation of the 15,000-dalton ige-suppressive factor and the 30,000-dalton ige-binding factor, which has neither potentiating activity nor suppressive activity on the ige response. another t cell hybridoma, 23a4 cells, produces the 30,000-dalton "inactive" ige-binding factor upon incubation with ige. both the 15,000-dalton ige-suppressive factor and the 30,000-dalton ige-binding factor lacked af ...	1983	6224847