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clinical characteristics of melon (cucumis melo) allergy.although melon is a frequent allergy-eliciting fruit, allergic reactions to melon have rarely been reported.200314533664
identification of cucumisin (cuc m 1), a subtilisin-like endopeptidase, as the major allergen of melon fruit.allergenic components in melon extracts have not been described in spite of the fact that melon (cucumis melo) is a frequent allergy-eliciting fruit. the aim of this study was to evaluate allergenic components in melon extract and to report the identification of cucumisin as a major melon allergen.200312801320
identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed bac library. microcolinearity between cucumis melo and arabidopsis thaliana.a bacterial artificial chromosome (bac) library from the dihaploid melon line 'pit92' was constructed with a 6 times coverage of the haploid melon genome. a contig of four bacs around the mrgh63 resistance gene homologue fragment was created. the complete sequence of a 117-kb bac clone allowed to determine two clearly defined regions, the first one containing a cluster of three resistance gene homologues. separated by a retrotransposon, that contains large long terminal repeats, the second regio ...200312678558
melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation.abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ...200318943165
binary transformation systems based on 'shooter' mutants of agrobacterium tumefaciens: a simple, efficient and universal gene transfer technology that permits marker gene elimination.a simple transformation procedure with a positive selection scheme using the expression of the isopentenyl transferase ( ipt) gene of transfer dna (t-dna) 'shooter' mutants of agrobacterium tumefaciens was elaborated. after comparing several 'shooter' mutants we found that particular strains frequently produced phenotypically normal shoots after co-culturing with tobacco leaf explants. shoots selected for normal phenotype showed apical dominance and could be rooted with the same efficiency as no ...200312789522
aflp analysis of a worldwide collection of didymella bryoniae.didymella bryoniae (anamorph phoma cucurbitacearum) is an ascomycete that causes gummy stem blight, a foliar disease that occurs on cucurbits in greenhouses and fields throughout the world. in a previous study using rapd analysis, little genetic diversity was found among isolates of d. bryoniae from new york and south carolina, usa. here we report the use of amplified fragment length polymorphism (aflp) analysis to assess the genetic variation within a worldwide collection of d. bryoniae, 102 fi ...200312825498
recovery of surface bacteria from and surface sanitization of cantaloupes.practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. cantaloupes (cucumis melo l. var. reticulates) were immersed in an inoculum containing salmonella enterica serovar poona or pantoea agglomerans at ca. 10(4) to 10(5) cfu/ml. an efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. the method consisted of wa ...200314572217
biocontrol of listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin.the fresh-cut produce industry has been the fastest-growing portion of the food retail market during the past 10 years, providing consumers with convenient and nutritious food. however, fresh-cut fruits and vegetables raise food safety concerns, because exposed tissue may be colonized more easily by pathogenic bacteria than intact produce. this is due to the higher availability of nutrients on cut surfaces and the greater potential for contamination because of the increased amount of handling. w ...200312902237
powdery mildew (sphaerotheca fuliginea) resistance in melon is selectable at the haploid level.the major cause of powdery mildew in melons (cucumis melo l.) is the fungus sphaerotheca fuliginea. there are several cultivar- and season-specific races of this fungus. in order to control powdery mildew, it is important to introduce resistance to fungal infection into new cultivars during melon breeding. haploid breeding is a powerful tool for the production of pure lines. in this study, it was investigated whether powdery mildew resistance could be manifested at the haploid level from two dis ...200312598576
on-farm and postharvest processing sources of bacterial contamination to melon rinds.multistate and international foodborne illness outbreaks, particularly involving cantaloupe and often involving rare salmonella spp., have increased dramatically over the past 13 years. this study assessed the sources and extent of melon rind contamination in production fields and at processing and packing facilities. in the spring of 1999, cantaloupe (cucumis melo l. [reticulatus group] cv. cruiser) sampled from two sites in the rio grande river valley showed that postharvest-processed melon ri ...200312540185
functional and phylogenetic analyses of a conserved regulatory program in the phloem of minor veins.the minor-vein phloem of mature leaves is developmentally and physiologically distinct from the phloem in the rest of the vascular system. phloem loading of transport sugars occurs in the minor veins, and consistent with this, galactinol synthase is expressed in the minor veins of melon (cucumis melo) as part of the symplastic-loading mechanism that operates in this species. a galactinol synthase promoter from melon drives gene expression in the minor-vein companion cells of both transgenic toba ...200314526110
nucleotide sequences of two korean isolates of cucumber green mottle mosaic virus.the nucleotide sequences of the genomic rnas of cucumber green mottle mosaic virus korean watermelon isolate (cgmmv-kw) and korean oriental melon isolate (cgmmv-kom) were determined and compared to the sequences of other tobamoviruses including cgmmv strains w and sh. each cgmmv isolate had a genome of 6,424 nucleotides. each also had 60 and 176 nucleotides of 5' and 3' untranslated regions (utrs), respectively, and four open reading frames (orf1-4). orfs 1 to 4 encode proteins of 129, 186, 29, ...200314744034
sensory and quality analysis of different melon cultivars after prolonged storage.the purpose of this work was to evaluate the sensory and general quality of four different melon cultivars (cucumis melo l.) immediately after harvest and at the end of storage and marketing simulation. after 16 days of storage at 5 degrees c and additional 3 days at 20 degrees c, only cultivar 'c-8' had a poor general appearance due to significant low firmness and relatively high decay incidence compared to the cultivars '5080', 'ideal' and '7302'. the cultivar '7302' was found to have the high ...200314609087
ozone increases root respiration but decreases leaf co2 assimilation in cotton and melon.it is well established that exposure of plant foliage to tropospheric ozone (o3) inhibits photosynthetic gas exchange in leaves and the translocation of current photosynthate to sink tissues. it is less clear what impact o3-reduced source strength has on the physiological responses of sink tissue such as fine roots. the responses were investigated of carbon acquisition in leaves and carbon utilization in the respiration of fine roots, following chronic (weeks) and acute (hours) exposures to o3 i ...200312947052
evaluation of aroma compounds contributing to muskmelon flavor in porapak q extracts by aroma extract dilution analysis.the flavor of the miyabi variety of japanese muskmelon was extracted according to the porapak q column method (pqm) and evaluated by using aroma extract dilution analysis (aeda) method. the overall odor of the pqm extracts was perceived as having a natural muskmelon-like odor, suggesting that the pqm was able to extract volatile compounds in muskmelon fruit without degradation of original flavor. forty-six odorant compounds [kovats index (ki), 961 < or = ki < or = 2605] were found by gc-sniffing ...200312744676
identification of 1,8-dihydroxynaphthalene melanin in monosporascus cannonballus and the analysis of hexaketide and pentaketide compounds produced by wild-type and pigmented isolates of the fungus.monosporascus cannonballus causes root rot and vine decline in muskmelons and watermelons. wild types of this fungus often undergo degenerative changes that have been associated with yellow to brown pigmentation, hypovirulence, dsrna infection, and decreased production of perithecia. in this study, degenerate isolates that produced yellow to brown pigments and no perithecia were obtained from wild-type cultures that had been stored for extended periods of time. cultures of the degenerate isolate ...200415212456
isolation and identification of hexaketides from a pigmented monosporascus cannonballus isolate.monosporascus cannonballus causes severe production losses to muskmelon and watermelon in the united states and other countries. wild types of the fungus produce no pigments when grown on potato dextrose agar (pda). after long-term storage on soil/oat hull mix, however, some isolates of the fungus produce yellow to brown pigments and no perithecia when grown on pda. five colored metabolites from pigmented cultures of m. cannonballus isolate tx923038 have now been identified. two of these, monosp ...200415212455
identification of quantitative trait loci involved in fruit quality traits in melon (cucumis melo l.).two populations [an f(2) and a set of 77 double haploid lines (dhls)] developed from a cross between a 'piel de sapo' cultivar (ps) and the exotic korean accession pi 161375 were used to detect qtls involved in melon fruit quality traits: earliness (ea), fruit shape (fs), fruit weight (fw) and sugar content (ssc); and loci involved in the colour traits: external colour (ecol) and flesh colour (fc). high variation was found, showing transgressive segregations for all traits. the highest correlati ...200414576985
comparative mapping of zymv resistances in cucumber (cucumis sativus l.) and melon (cucumis melo l.).zucchini yellow mosaic virus (zymv) routinely causes significant losses in cucumber ( cucumis sativus l.) and melon ( cucumis melo l.). zymv resistances from the cucumber population 'tmg1' and the melon plant introduction (pi) 414723 show different modes of inheritance and their genetic relationships are unknown. we used molecular markers tightly linked to zymv resistances from cucumber and melon for comparative mapping. a 5-kb genomic region (ycz-5) cosegregating with the zym locus of cucumber ...200415340688
janibacter melonis sp. nov., isolated from abnormally spoiled oriental melon in korea.two gram-positive bacterial strains, cm2104(t) and cm2110, isolated from the inner part of abnormally spoiled oriental melon (cucumis melo) in korea, were subjected to a polyphasic taxonomic study. the cell-wall peptidoglycan of strains cm2104(t) and cm2110 contained meso-diaminopimelic acid as the diagnostic diamino acid. the predominant menaquinone was mk-8(h(4)). the major fatty acids detected in the two strains were iso-c(16 : 0), c(17 : 1)omega8c and c(18 : 1)omega9c or c(17 : 0). the dna g ...200415545420
cerebroside elicitor confers resistance to fusarium disease in various plant species.abstract in the rice blast fungus pathosystem, cerebroside, a compound categorized as a sphingolipid, was found in our previous study to be a non-racespecific elicitor, which elicits defense responses in rice. here we describe that cerebroside c is produced in diverse strains of fusarium oxysporum, a common soilborne agent of wilt disease affecting a wide range of plant species. in addition, some type of cerebroside elicitor involving cerebroside a, b, or c was detected in other soilborne phytop ...200418943100
induced systemic resistance and promotion of plant growth by bacillus spp.abstract elicitation of induced systemic resistance (isr) by plant-associated bacteria was initially demonstrated using pseudomonas spp. and other gram-negative bacteria. several reviews have summarized various aspects of the large volume of literature on pseudomonas spp. as elicitors of isr. fewer published accounts of isr by bacillus spp. are available, and we review this literature for the first time. published results are summarized showing that specific strains of the species b. amyloliquef ...200418944464
effect of hot water and hydrogen peroxide treatments on survival of salmonella and microbial quality of whole and fresh-cut cantaloupe.cantaloupe melon has been associated with outbreaks of salmonellosis. contamination might be introduced into the flesh from the rind by cutting or by contact of cut pieces with contaminated rinds. our objectives were to investigate the efficacy of hot water or hot 5% hydrogen peroxide treatments in reducing the population of native microflora and inoculated salmonella on cantaloupe rind and transfer to fresh-cut tissue during cutting. whole cantaloupes, inoculated with a cocktail of salmonella s ...200415035354
five independent loci each control monogenic resistance to gummy stem blight in melon ( cucumis melo l.).inheritance and segregation analysis demonstrated that five independent genes in melon confer monogenic resistance to foliar infection by the fungal pathogen didymella bryoniae, resulting in the disease known as gummy stem blight (gsb). in this study, two new monogenic sources of gsb resistance were characterized. resistance in cucumis melo pi 482398 was monogenic dominant based on segregation analysis of f(1), f(2) and backcross populations, while resistance in c. melo pi 482399 showed monogeni ...200415067389
phloem specific expression driven by wheat dwarf geminivirus v-sense promoter in transgenic dicotyledonous species.wheat dwarf geminivirus (wdv) is a single-stranded dna mastrevirus. the large intergenic region (lir) of wdv contains cis-acting elements essential for the replication of the genome as well as for the bidirectional transcription of virus genes. the lir was fused to the gus (uida) reporter gene and the wdv viral sense (v-sense) promoter activity derived from the stable integration of that promoter was analysed in transgenic dicot plants. various dicot species were tested, including nicotiana taba ...200415086824
molecular characterization of a melon necrotic spot virus strain that overcomes the resistance in melon and nonhost plants.resistance of melon (cucumis melo l.) to melon necrotic spot virus (mnsv) is inherited as a single recessive gene, denoted nsv. no mnsv isolates described to date (e.g., mnsv-malpha5), except for the mnsv-264 strain described here, are able to overcome the resistance conferred by nsv. analysis of protoplasts of susceptible (nsv/-) and resistant (nsv/nsv) melon cultivars inoculated with mnsv-264 or mnsv-malpha5 indicated that the resistance trait conferred by this gene is expressed at the single- ...200415195949
investigation of volatiles in charentais cantaloupe melons (cucumis melo var. cantalupensis). characterization of aroma constituents in some cultivars.volatile compounds of 15 charentais melon cultivars, known to exhibit differences in their ripening behaviors and in their storage lives (wild, mid, and long shelf life), were investigated. twenty-eight volatiles (11 esters, 8 sulfur compounds, 6 alcohols, and 3 carbonyl compounds) were isolated by direct dichloromethane extraction and analyzed by means of gc-ms and gc-fid. a considerable reduction in the aroma profile was observed for the long shelf life cultivars, in which total volatiles were ...200415237961
effect of hydrogen peroxide treatment on microbial quality and appearance of whole and fresh-cut melons contaminated with salmonella spp.the efficacy of hydrogen peroxide treatment on the inactivation of salmonella spp. inoculated on the external surface of cantaloupe and honeydew melon was investigated. salmonella was inoculated onto whole cantaloupe and honeydew melon to a final concentration of 4.65 log(10) cfu/cm(2) and 3.13 log(10) cfu/g, respectively. inoculated whole melons stored at 5 degrees c for up to 7 days were washed with water, 2.5% and 5% hydrogen peroxide at day 0 and 5. hydrogen peroxide (2.5% and 5%) treatments ...200415282126
optimizing concentration and timing of a phage spray application to reduce listeria monocytogenes on honeydew melon tissue.a phage cocktail was applied to honeydew melon pieces 1, 0.5, and 0 h before contamination with listeria monocytogenes strain lcdc 81-861 and 0.5, 1, 2, and 4 h after contamination. the phage application was most effective when applied 1, 0.5, or 0 h before contamination with l. monocytogenes, reducing pathogen populations by up to 6.8 log units after 7 days of storage. this indicates that under commercial conditions, if contamination occurs at the time of cutting, phage would have to be applied ...200415330534
[effects of exogenous gamma-aminobutyric acid on antioxidant enzyme activity and reactive oxygen content in muskmelon seedlings under nutrient solution hypoxia stress].the effects of exogenous gamma-aminobutyric acid (gaba) on antioxidant defense system and reactive oxygen species (ros) in seedlings of two varieties with different resistance to hypoxia stress were studied under hypoxia stress in a nutrient solution culture system. the results showed that gaba treatments enhanced the activities of antioxidant enzyme including sod (fig. 1), pod (fig. 2), cat (fig. 3), while the concentrations of h2o2 (fig. 4), o(-.)2 (fig. 5), mda (fig. 6) decreased under hypoxi ...200415643085
antioxidant and anti-inflammatory properties of a cucumis melo lc. extract rich in superoxide dismutase activity.the present study was conducted to evaluate in vitro and in vivo the antioxidant and anti-inflammatory properties of a cantaloupe melon (cucumis melo lc., cucurbitaceae) extract (cme) selected for its high superoxide dismutase activity. peritoneal macrophages were pre-activated in vitro with 300 iu of interferon-gamma (ifn-gamma) and were then challenged in culture with iggl/anti-igg1 immune complexes (igg1ic) in presence of various cme extracts. the subsequent production of free radicals (super ...200415261965
molecular characterization and expression studies during melon fruit development and ripening of l-galactono-1,4-lactone dehydrogenase.the last step of ascorbic acid (aa) biosynthesis is catalysed by the enzyme l-galactono-1,4-lactone dehydrogenase (galldh, ec 1.3.2.3), located on the inner mitochondrial membrane. the enzyme converts l-galactono-1,4-lactone to ascorbic acid (aa). in this work, the cloning and characterization of a galldh full-length cdna from melon (cucumis melo l.) are described. melon genomic dna southern analysis indicated that cmgalldh was encoded by a single gene. cmgalldh mrna accumulation was detected in ...200415234991
cloning of genes differentially expressed during the initial stage of fruit development in melon (cucumis melo cv. reticulatus).we have cloned genes involved in the initial stage of fruit development in the melon by suppression subtractive hybridization. a cdna library of unfertilized ovules was subtracted from that of fruit 9 days after pollination (dap); 10 of the 40 selected cdna clones were identified by reverse northern analysis as genes differentially expressed in fruit at 9 dap. seven of the ten genes were homologous to genes of known function; two were related to genes with unknown functions, and one was novel. w ...200415179036
promotion of 5-aminolevulinic acid on photosynthesis of melon (cucumis melo) seedlings under low light and chilling stress conditions.when melon seedlings (cucumis melo l. ximiya no. 1) were cultured in a growth chamber with about 150 micro mol m(-2) s(-1) photon flux density, the leaf photosynthetic ability reduced dramatically as leaf position decreased from the top. the application of 5-aminolevulinic acid (ala) solutions significantly increased the net photosynthetic rate (p(n)) as well as apparent quantum yield (aqy), carboxylation efficiency (ce) and stomata conductance (g(s)). after irrigation with 10 ml of ala solution ...200415153193
development of microsatellite markers from an enriched genomic library for genetic analysis of melon (cucumis melo l.).despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (cucumis melo l.) and cucurbit species. the development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs. genomic microsatellite enriched libraries can be an eff ...200415149552
peroxidase activity associated with suberization processes of the muskmelon (cucumis melo) rind.the rind of fruits of muskmelon (cucumis melo l. var. reticulatus) contains a network of suberized tissue referred to as the 'netting', and peroxidase (ec 1.11.1.7) activity is necessary to the polymerization of the aromatic domain of suberin. peroxidase activity increased dramatically during the early stages of melon fruit netting, and in fruits exhibiting incomplete netting, peroxidase activity was significantly higher in netted than in non-netted regions of the same fruit. moreover, analysis ...200415086828
pre-extraction preparation (fresh, frozen, freeze-dried, or acetone powdered) and long-term storage of fruit and vegetable tissues: effects on antioxidant enzyme activity.activities of the antioxidant enzymes ascorbate peroxidase, catalase, dehydroascorbate reductase, glutathione reductase, guaiacol peroxidase, monodehydroascorbate reductase, and superoxide dismutase were assayed in honeydew (cucumis melo l.) fruit and spinach (spinacia oleracea l.) leaves either as fresh, frozen to -80 degrees c, frozen in liquid nitrogen, freeze-dried, or acetone powder, representing the various ways tissues are treated prior to enzyme extraction. treated tissues were analyzed ...200415080616
single-nucleotide polymorphisms detected in expressed sequence tags of melon (cucumis melo l.).a search was performed for single-nucleotide polymorphisms (snp) and short insertions-deletions (indels) in 34 melon (cucumis melo l.) expressed sequence tag (est) fragments between two distantly related melon genotypes, a group inodorus 'piel de sapo' market class breeding line t111 and the korean accession pi 161375. in total, we studied 15 kb of melon sequence. the average frequency of snps between the two genotypes was one every 441 bp. one indel was also found every 1666 bp. seventy-five pe ...200415060588
development of a genomic library of near isogenic lines (nils) in melon (cucumis melo l.) from the exotic accession pi161375.a doubled haploid line (dhl) population of melon derived from a cross between the korean cultivar "songwhan charmi" accession pi161375 (sc), included in the horticultural group conomon, and the spanish cultivar "piel de sapo" (ps), included in the horticultural group inodorus, was used to develop a collection of near isogenic lines (nils). these parental lines represent very different melon cultivar groups, with important differences at fruit, plant, disease response and molecular level. this cr ...200516208502
analysis of the melon genome in regions encompassing tir-nbs-lrr resistance genes.plant genomes contain numerous genes (r-genes) that play a role in initiating defence measures against their particular pathogens. defence mechanisms controlled by r-genes have been the focus of extensive research over the past several years. the majority of the r-genes described so far belong to a super-family of genes (150-600 members) that encode proteins with a nucleotide binding site (nbs), some leucine-rich repeats (lrr) and an n-terminal domain that shows similarity to the toll and interl ...200515902490
simple-sequence repeat markers used in merging linkage maps of melon (cucumis melo l.).a set of 118 simple sequence repeat (ssr) markers has been developed in melon from two different sources: genomic libraries (gssr) and expressed sequence-tag (est) databases (est-ssr). forty-nine percent of the markers showed polymorphism between the 'piel de sapo' (ps) and pi161375 melon genotypes used as parents for the mapping populations. similar polymorphism levels were found in gssr (51.2%) and est-ssr (45.5%). two populations, f2 and a set of double haploid lines (dhls), developed from th ...200515700148
the melon fruit fly, bactrocera cucurbitae: a review of its biology and management.the melon fruit fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae) is distributed widely in temperate, tropical, and sub-tropical regions of the world. it has been reported to damage 81 host plants and is a major pest of cucurbitaceous vegetables, particularly the bitter gourd (momordica charantia), muskmelon (cucumis melo), snap melon (c. melo var. momordica), and snake gourd (trichosanthes anguina). the extent of losses vary between 30 to 100%, depending on the cucurbit species and ...200517119622
use of hydrogen peroxide in combination with nisin, sodium lactate and citric acid for reducing transfer of bacterial pathogens from whole melon surfaces to fresh-cut pieces.hydrogen peroxide (2.5%) alone or hydrogen peroxide (1%) in combination with nisin (25 microg/ml), sodium lactate (1%), and citric acid (0.5%) (hplnc) were investigated as potential sanitizers for reducing escherichia coli o157:h7 or listeria monocytogenes populations on whole cantaloupe and honeydew melons. whole cantaloupes inoculated with e. coli o157:h7 and l. monocytogenes at 5.27 and 4.07 log10 cfu/cm2, respectively, and whole honeydew melons inoculated with e. coli o157:h7 and l. monocyto ...200516043249
reduction of diabetes-induced renal oxidative stress by a cantaloupe melon extract/gliadin biopolymers, oxykine, in mice.oxidative stress is implicated as an important mechanism by which diabetes causes nephropathy. oxykine is the cantaloupe melon extract rich in vegetal superoxide dismutase covered by polymeric films of wheat matrix gliadin. in this study, we examined whether chronic oral administration of oxykine could prevent the progression of diabetic nephropathy induced by oxidative stress using preclinical rodent model of type 2 diabetes. we used female db/db mice and their non-diabetic db/m littermates. th ...200516179750
functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. identification of the crucial role of a threonine residue for enzyme activity*.volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases (aat). we demonstrate here that, in charentais melon (cucumis melo var. cantalupensis), aat are encoded by a gene family of at least four members with amino acid identity ranging from 84% (cm-aat1/cm-aat2) and 58% (cm-aat1/cm-aat3) to only 22% (cm-aat1/cm-aat4). all encoded proteins, except cm-aat2, were enzymatically active upon expression in yeast and show different ...200516247561
atp bioluminescence assay for estimation of microbial populations of fresh-cut melon.estimation of microbial numbers in foods by conventional microbiological techniques takes days, so there is a need for faster methods that can give results in minutes. research was undertaken to investigate the use of bioluminescent atp determination and a firefly luciferase assay to estimate the initial population of aerobic mesophilic bacteria on fresh-cut melons immediately after preparation and during storage at 5 or 15 degrees c for up to 12 days. populations of aerobic mesophilic bacteria ...200516300083
inheritance of resistance to watermelon mosaic virus in cucumis melo that impairs virus accumulation, symptom expression, and aphid transmission.abstract the cucumis melo accession tgr-1551 was found to be resistant to watermelon mosaic virus (wmv, genus potyvirus, family potyviridae). the resistance resulted in a drastic and significant reduction of virus titer and infected plants were asymptomatic or exhibited mild disease symptoms. the same gene or closely linked genes restricted virus accumulation and ameliorated symptom expression. no effect was observed on virus accumulation in inoculated leaves, which suggested that the initial ph ...200518943018
molecular markers linked to papaya ring spot virus resistance and fusarium race 2 resistance in melon.in melon, the fom-1 gene confers monogenic resistance against the soil-borne fungus fusarium oxysporum f. sp. melonis, races 0 and 2, while the closely linked prv gene specifies resistance against the papaya ring spot virus. markers linked to these resistance (r) genes were identified using two recombinant inbred line populations, derived from crosses between cucumis melo vedrantais and c. melo pi 161375, and between c. melo vedrantais and c. melo pi 414723, respectively. using bulked segregant ...200515551034
a physical map covering the nsv locus that confers resistance to melon necrotic spot virus in melon (cucumis melo l.).melon necrotic spot virus (mnsv) is a member of the genus carmovirus, which produces severe yield losses in melon and cucumber crops. the nsv gene is the only known natural source of resistance against mnsv in melon, and confers protection against all widespread strains of this virus. nsv has been previously mapped in melon linkage group 11, in a region spanning 5.9 cm, saturated with rapd and aflp markers. to identify the nsv gene by positional cloning, we started construction of a high-resolut ...200516052354
reducing salmonella on cantaloupes and honeydew melons using wash practices applicable to postharvest handling, foodservice, and consumer preparation.washing conditions that included a soak or brush scrub were evaluated for removal of salmonella from the surface of smooth (honeydew) or complex (cantaloupe) melon rinds. melon rinds were spot-inoculated onto a 2.5 cm2 area of rind (squares) with approximately 6.0 log(10) cfu/square of an avirulent nalidixic acid-resistant strain of salmonella typhimurium. melons were washed by immersion in 1500 ml of water or 200 ppm total chlorine and allowed to soak or were scrubbed over the entire melon surf ...200515718029
strain-specific and recessive qtls involved in the control of partial resistance to fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon.fusarium oxysporum f. sp. melonis (fom) causes serious economic losses in melon (cucumis melo l.). two dominant resistance genes have been identified, fom-1 and fom-2, which provide resistance to races 0 and 2 and races 0 and 1, respectively, however fom race 1.2 overcomes these resistance genes. a partial resistance to fom race 1.2 that has been found in some far east accessions is under polygenic control. a genetic map of melon was constructed to tag fom race 1.2 resistance with dna markers on ...200515834544
bacteremia caused by janibacter melonis.we report a case of bacteremia caused by janibacter melonis, a recently described aerobic actinomycete originally isolated from a spoiled oriental melon. our patient's blood culture isolate was identified by partial 16s rrna gene sequencing. this is the first report of the recovery of janibacter species from humans.200516000500
methylene blue staining method for identification of opisthorchis viverrini egg.methylene blue staining method was used to distinguish o. viverrini eggs from haplorchis taichui and prosthodendrium molenkampi eggs. all eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. the distinct musk-melon-like texture of the o. viverini eggshell surface and the thread-like texture of h. taichui eggshell surface were recognized, while p. molenkampi eggs showed a smooth eggshell. we also evaluated the ...200516438191
impact of melon accessions resistant to aphids on the demographic potential of silverleaf whitefly.bemisia tabaci (gennadius) and aphis gossypii glover are devastating melon, cucumis melo l., pests. the geographic areas where they occur overlap, and the same chemicals are used to control both of them. therefore, to reduce pesticide use, it would be necessary to breed melon lines that simultaneously express a resistance to both insects. female survival; the time when reproduction starts, peaks, and ends; the number of female offspring at the reproductive peak; and total reproduction (s) were d ...200515889749
effects of application strategies of fumigant and nonfumigant nematicides on cantaloupe grown in deep sand soils in florida.a 2-year study was conducted in which three treatment tactics of oxamyl (at planting application, application every 2 weeks, and rescue applications, as determined by crop symptoms) were compared to fumigant treatments with methyl bromide, 1,3-dichloropropene (1,3-d), and 1,3-d plus chloropicrin for management of meloidogyne spp. in 2002, treatments that included 1,3-d produced higher yields as determined both by number and weight of marketable fruit. all treatment tactics relying solely on oxam ...200519262874
use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita.root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ...200519262873
the perisperm-endosperm envelope in cucumis: structure, proton diffusion and cell wall hydrolysing activity.and aims the envelope surrounding the embryo in cucurbit seed, which consists of a single layer of live endosperm cells covered by lipid- and callose-rich layers, is reported to show semi-permeability and also to act as the primary barrier to radicle emergence. structure, development and permeability of the envelope and activity of cell wall hydrolases during germination of cucumber and muskmelon seeds were investigated.200516043437
the wide distribution of endornaviruses, large double-stranded rna replicons with plasmid-like properties.the international committee on taxonomy of viruses (ictv) recently accepted endornavirus as a new genus of plant dsrna virus. we have determined the partial nucleotide sequences of the rna-dependent rna polymerase regions from the large dsrnas (about 14 kbp) isolated from barley (hordeum vulgare), kidney bean (phaseolus vulgaris), melon (cucumis melo), bottle gourd (lagenaria siceraria), malabar spinach (basella alba), seagrass (zostera marina), and the fungus helicobasidium mompa. phylogenetic ...200616341944
immunolocalization of a class iii chitinase in two muskmelon cultivars reacting differently to fusarium oxysporum f. sp. melonis.fusarium oxysporum f. sp. melonis is a highly specialized fungus that attacks the root system of melon (cucumis melo l.). in this work the presence of a class iii chitinase was examined by immunological techniques in the root and stem base of a susceptible (cv. galia) and a resistant (cv. bredor) melon during the infection process. by immunolocalization it was not possible to detect the constitutive presence of class iii chitinase in any of the cultivars. however, the immunolabelling appeared in ...200616360800
transformation of a muskmelon 'galia' hybrid parental line (cucumis melo l. var. reticulatus ser.) with an antisense acc oxidase gene.'galia' muskmelon (cucumis melo l. var. reticulatus ser.) has been recalcitrant to transformation by agrobacterium tumefaciens. transformation of the 'galia' male parental line, 'krymka', with an acc oxidase (cmaco-1) gene in antisense orientation is described herein. explants were transformed using a. tumefaciens strain abi, which contained a vector pcmaco1-as plasmid, bearing an antisense gene of cmaco-1 and the cp4 syn gene (glyphosate-tolerance). both cmaco-1 and cp4 syn genes were assessed ...200616362302
cloning and expression analysis of a udp-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants.the cucurbitaceae translocate a significant portion of their photosynthate as raffinose and stachyose, which are galactosyl derivatives of sucrose. these are initially hydrolyzed by alpha-galactosidase to yield free galactose (gal) and, accordingly, gal metabolism is an important pathway in cucurbitaceae sink tissue. we report here on a novel plant-specific enzyme responsible for the nucleotide activation of phosphorylated gal and the subsequent entry of gal into sink metabolism. the enzyme was ...200616829585
two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics.alcohol dehydrogenases (adh) participate in the biosynthetic pathway of aroma volatiles in fruit by interconverting aldehydes to alcohols and providing substrates for the formation of esters. two highly divergent adh genes (15% identity at the amino acid level) of cantaloupe charentais melon (cucumis melo var. cantalupensis) have been isolated. cm-adh1 belongs to the medium-chain zinc-binding type of adhs and is highly similar to all adh genes expressed in fruit isolated so far. cm-adh2 belongs ...200616897483
hydroperoxide lyases (cyp74c and cyp74b) catalyze the homolytic isomerization of fatty acid hydroperoxides into hemiacetals.the conversion of linoleic acid 9-hydroperoxide (9-hpod) by recombinant melon (cucumis melo l.) hydroperoxide lyase (hpl, cyp74c subfamily) was studied. short (5 s-1 min) incubations at 0 degrees c followed by rapid extraction and trimethylsilylation made it possible to trap a new unstable (t(1/2) <30 s) product, i.e. the hemiacetal (1'e,3'z)-9-hydroxy-9-(1',3'-nonadienyloxy)-nonanoic acid. identification was performed by gc-ms analysis and substantiated by the formation of trimethylsilyl 9-trim ...200617049304
functional characterization of cmccd1, a carotenoid cleavage dioxygenase from melon.carotenoids are nutritionally important tetraterpenoid pigments that upon oxidative cleavage give rise to apocarotenoid (norisoprene) aroma volatiles. beta-carotene is the predominant pigment in orange-fleshed melon (cucumis melo l.) varieties, reaching levels of up to 50 microg/gfw. pale green and white cultivars have much lower levels (0-10 microg/gfw). in parallel, beta-ionone, the 9,10 cleavage product of beta-carotene, is present (12-33ng/gfw) in orange-fleshed melon varieties that accumula ...200616563447
pcr identification of salmonella: potential contamination sources from production and postharvest handling of cantaloupes.salmonella is one of the most frequently reported etiological agents in outbreaks of foodborne diseases associated with the consumption of cantaloupes. sensitive and reliable methods for detecting and identifying foodborne microorganisms are needed. the pcr can be used to amplify specific dna fragments and thus to detect and identify pathogenic bacteria. in this study, a pcr method was used to evaluate the incidence of salmonella at cantaloupe production, harvest, and packaging steps, and the re ...200616786866
volatile compounds in the skin and pulp of queen anne's pocket melon.the quantitative distribution of volatile compounds in the skin and pulp of queen anne's pocket melon [cucumis melo var. dudaim (l.) naudin] has been investigated. volatile compounds were extracted by liquid-liquid microextraction (llme) using chloroform and analyzed by gc-fid and gc-ms. sixty volatiles, including 20 esters, 15 alcohols, 7 lactones, 7 aldehydes and ketones, 6 sulfur compounds, and 5 c(6) compounds, have been identified. among them, 38 were reported for the first time in pocket m ...200617032026
comparative analysis of plant finds from early roman graves in ilok (cuccium) and sćitarjevo (andautonia), croatia--a contribution to understanding burial rites in southern pannonia.a comparative archaeobotanical analysis of the plant remains from the early roman incineration graves in ilok and sćitarjevo shows the existence of a complex burial ritual, but at the same time enables a better understanding of the agriculture and trade of the 1st/early 2nd century ad in southern pannonia. most of the cereals found (hordeum vulgare, panicum miliaceum, triticum monococcum, t. dicoccon, t. aestivum i t. cf. spelta), the legumes (lens culinaris, vicia ervilia) and the fruit contrib ...200616848163
subcellular localization and membrane topology of the melon ethylene receptor cmers1.ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. to better understand their biochemical functions, we investigated the membrane topology and subcellular localization of cmers1, a melon (cucumis melo) ethylene receptor that has three putative transmembrane domains at the n terminus. analyses using membrane fractionation and green fluorescent protein imaging approaches indicate that ...200616617090
purification and characterization of a serine protease from cucumis trigonus roxburghi.kachri fruit, cucumis trigonus roxburghi, contains high protease activity and has been used as meat tenderizer in the indian subcontinent. a 67 kda serine protease from kachri fruit was purified by deae-sepharose and cm-sepharose chromatography, whose optimum activity was at ph 11 and 70 degrees c. its activity was strongly inhibited by pmsf, but not by edta, pepstatin, or cysteine protease inhibitors. the substrate specificity of the purified protease towards synthetic peptides was comparable t ...200616603211
development of loop-mediated isothermal amplification (lamp)-based snp markers for shelf-life in melon (cucumis melo l.).in this study, lamp markers linked to shelf-life in melon (cucumis melo l.) were developed by converting a cleaved amplified polymorphic sequences (caps) marker (c2). the caps-pcr fragments from the long-shelf-life melon (o-3) and short-shelf-life melon (nat-2) were cloned and sequenced to construct lamp primers. a single nucleotide polymorphism (snp) was identified between o-3 and nat-2. lamp primers were designed to detect the snp. in the lamp reaction to detect long-shelf-life melon, the turb ...200617132894
[anthelmintic properties of cucumis melo seeds]. 200613294200
molecular phylogeny of cucumis species as revealed by consensus chloroplast ssr marker length and sequence variation.to investigate phylogenetic relationships in the genus cucumis, 9 consensus chloroplast simple sequence repeat (ccssr) primer pairs (ccssr3, 9, 11, 13, 14, 17, 20, 21, and 23) were employed for dna fragment length variation and 5 amplified fragments, ccssr4, 12, 13, 19, and 20, were sequenced using total dna from 13 accessions representing 7 african cucumis species (x = 12), 3 cucumis melo l. (x = 12) accessions, 2 cucumis sativus l. (x = 7) accessions, and 1 cucumis hystrix chakr. (x = 12) acce ...200616604104
the cucurbit images (1515-1518) of the villa farnesina, rome.the gorgeous frescoes organized by the master renaissance painter raphael sanzio (1483-1520) and illustrating the heavenly adventures of cupid and psyche were painted between 1515 and 1518 to decorate the roman villa (now known as the villa farnesina) of the wealthy sienese banker agostino chigi (1466-1520). surrounding these paintings are festoons of fruits, vegetables and flowers painted by giovanni martini da udine (1487-1564), which include over 170 species of plants. a deconstruction and co ...200616314340
differential expression of the ascorbate oxidase multigene family during fruit development and in response to stress.ascorbate oxidase (ao, ec 1.10.3.3) is a member of the multicopper oxidases family. it catalyzes the oxidation of ascorbic acid (aa) to dehydroascorbic acid (dha) via monodehydroascorbate (mdha), with the concomitant reduction of molecular oxygen to water. in melon (cucumis melo), ascorbate oxidase is encoded by a multigene family comprising at least four genes. here, we present the detailed characterization of two melon ao genes, cmao1 and cmao4. gene-specific expression studies of the ao gene ...200717021803
gel electrophoresis of polyphenol oxidase with instant identification by in situ blotting.polyphenol oxidase (ppo) or tyrosinase is an important and ubiquitous enzyme responsible for browning in plants and melanization in animals. the molecular size of the plant ppo is varied among the species and its activity can be enhanced by a variety of anionic detergents. in the present study, we developed a simple method for the first-step identification of ppo in fruit and vegetable extracts. first, 3mm chromatographic paper was immersed in 0.5% (w/v) catechol solution as an immobilized ppo s ...200716973425
herbal medicines as diuretics: a review of the scientific evidence.there is increasing interest in the health and wellness benefits of herbs and botanicals. this is with good reason as they might offer a natural safeguard against the development of certain conditions and be a putative treatment for some diseases. one such area may be the lowering of blood pressure in those where it is elevated (i.e., hypertension). one class of clinical medicines used to lower blood pressure are known as diuretics and work by increasing the excretion of urine from the body as w ...200717804183
melogen: an est database for melon functional genomics.melon (cucumis melo l.) is one of the most important fleshy fruits for fresh consumption. despite this, few genomic resources exist for this species. to facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ests) from eight normalized cdna libraries from different tissues in di ...200717767721
correlating volatile compounds, sensory attributes, and quality parameters in stored fresh-cut cantaloupe.changes in post-cutting volatiles, quality, and sensory attributes during fresh-cut storage (4 degrees c) of cantaloupe (cucumis melo l. var. reticulatus, naudin, cv. 'sol real') harvested at four distinct maturities (1/4-, 1/2-, 3/4-, and full-slip) were investigated after 0, 2, 5, 7, 9, 12, and 14 days in a 2-year study. increased fruity and sweet taste attributes were negatively correlated with percent acetates, aromatic acetates, and total aromatic compounds, and positively correlated with p ...200717944532
phylogenetics of cucumis (cucurbitaceae): cucumber (c. sativus) belongs in an asian/australian clade far from melon (c. melo).melon, cucumis melo, and cucumber, c. sativus, are among the most widely cultivated crops worldwide. cucumis, as traditionally conceived, is geographically centered in africa, with c. sativus and c. hystrix thought to be the only cucumis species in asia. this taxonomy forms the basis for all ongoing cucumis breeding and genomics efforts. we tested relationships among cucumis and related genera based on dna sequences from chloroplast gene, intron, and spacer regions (rbcl, matk, rpl20-rps12, trnl ...200717425784
detection of qtl for yield-related traits using recombinant inbred lines derived from exotic and elite us western shipping melon germplasm.the inheritance of yield-related traits in melon (cucumis melo l.; 2n = 2x = 24) is poorly understood, and the mapping of quantitative trait loci (qtl) for such traits has not been reported. therefore, a set of 81 recombinant inbred lines (ril) was developed from a cross between the monoecious, highly branched line usda 846-1 and a standard vining, andromonoecious cultivar, 'top mark'. the ril, parental lines, and three control cultivars ('esteem', 'sol dorado', and 'hales best jumbo') were grow ...200717294163
characterization of a regional population of fusarium oxysporum f. sp. niveum by race, cross pathogenicity, and vegetative compatibility.abstract eighty-eight isolates of fusarium oxysporum f. sp. niveum, collected from wilted watermelon plants and infested soil in maryland and dela-ware, were characterized by cross pathogenicity to muskmelon, race, and vegetative compatibility. four isolates (4.5%) were moderately pathogenic to >/=2 of 18 muskmelon cultivars in a greenhouse test, and one representative isolate also was slightly pathogenic in field microplots. the four isolates all were designated as race 2, and were in vegetativ ...200718943287
monitoring the incidence and causes of diseases potentially transmitted by food in australia: annual report of the ozfoodnet network, 2006.in 2006, ozfoodnet sites reported 24,598 notifications of seven diseases or conditions that are commonly transmitted by food, representing an increase of 2.5% over the mean of the previous 5 years. the most frequently notified aetiological agents were campylobacter (15,492 notifications) and salmonella (8,331 notifications). salmonella notifications increased in 2006 by 5.2% when compared to historical reports. the most common salmonella serotype notified in australia during 2006 was salmonella ...200718268875
microbial survey of selected ontario-grown fresh fruits and vegetables.recent produce-related outbreaks have been receiving heightened media coverage, which has increased public concern toward the safety of fresh fruits and vegetables. in response, the microbial contamination of ontario-grown fresh fruits and vegetables was evaluated by the ontario ministry of agriculture, food and rural affairs during the summer of 2004. prior to this survey, information specific to the microbial contamination of ontario-produced fruits and vegetables was limited. this nonregulato ...200718095445
induction of a small heat shock protein and its functional roles in nicotiana plants in the defense response against ralstonia solanacearum.in tobacco (nicotiana tabacum), ralstonia solanacearum oe1-1 (rsoe1-1) is pathogenic, whereas r. solanacearum 8107 (rs8107) is nonpathogenic and induces the hypersensitive response (hr). to elucidate the molecular mechanisms of plant-r. solanacearum interactions, we used differential display to isolate a cdna fragment, a6, regulated in tobacco by inoculation with rsoe1-1. the deduced amino acid sequence predicted from full-length a6-cdna showed similarity to small heat shock proteins from arabid ...200717965181
effect of time before storage and storage temperature on survival of salmonella inoculated on fresh-cut melons.the effects of a waiting period at room temperature ( approximately 22 degrees c) before refrigerating fresh-cut watermelon, cantaloupe and honeydew pieces contaminated with salmonella on survival of the inoculated pathogen were investigated. whole cantaloupes, honeydew melons and watermelons were washed with water, and fresh-cut pieces from individual melons were prepared and inoculated with a five strain cocktail of salmonella at 10(5)cfu/ml. populations of aerobic mesophilic bacteria, yeast a ...200717188207
effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping.we previously reported in healthy volunteers that a cantaloupe melon extract chemically combined with wheat gliadin (melon extract/gliadin) and containing sod, catalase and residual glutathione peroxidase (gpx), protected against dna strand-break damage induced by hyperbaric oxygen (hbo), a well-established model of dna damage resulting from oxidative stress. aortic cross-clamping is a typical example of ischemia/reperfusion injury-related oxidative stress, and therefore we investigated whether ...200717237934
effect of hand wash agents on controlling the transmission of pathogenic bacteria from hands to food.the goals of this study were to evaluate the effectiveness of two hand wash regimens in reducing transient bacteria on the skin following a single hand wash and the subsequent transfer of the bacteria to a ready-to-eat food item, freshly cut cantaloupe melon. the number of bacteria recovered from hands and the quantity transferred to the melon were significantly less following the use of an antibacterial soap compared with plain soap. the antimicrobial soap achieved > 3-log reductions versus esc ...200718095447
ethylene regulation of fruit softening and cell wall disassembly in charentais melon.cell wall disassembly in ripening fruit is highly complex, involving the dismantling of multiple polysaccharide networks by diverse families of wall-modifying proteins. while it has been reported in several species that multiple members of each such family are expressed in the same fruit tissue, it is not clear whether this reflects functional redundancy, with protein isozymes from a single enzyme class performing similar roles and contributing equally to wall degradation, or whether they have d ...200717308329
one-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation.a simple one-step method for the purification of recombinant his-tagged profilin from the bacterial cell lysate is reported. noting the greater ease with which hexahistidine-tagged proteins can be metalprecipitated compared with unwanted protein impurities, we investigated the effect of lysis-buffer additives and optimization of other conditions to recover selectively desired proteins in a one-step metal precipitation without using biopolymers. purification of the his-tagged melon (cucumis melo) ...200717313372
mutator-like elements identified in melon, arabidopsis and rice contain ulp1 protease domains.the transposon mutator was first identified in maize, and is one of the most active mobile elements in plants. the arabidopsis thaliana genome contains at least 200 mutator-like elements (mules), which contain the mutator-like transposase gene, and often additional genes. we have detected a novel type of mules in melon (cumule), which, besides the transposase, contains two ubiquitin-like specific protease-like sequences (ulp1). this element is not present in the observed location in some melon c ...200717136348
the cucurbits of mediterranean antiquity: identification of taxa from ancient images and descriptions.a critical analysis was made of cucurbit descriptions in dioscorides' de materia medica, columella's de re rustica and pliny's historia naturalis, works on medicine, agriculture and natural science of the 1st century ce, as well as the mishna and tosefta, compilations of rabbinic law derived from the same time period together with cucurbit images dating from antiquity including paintings, mosaics and sculpture. the goal was to identify taxonomically the mediterranean cucurbits at the time of the ...200717932073
[bee (apis mellifera, hymenoptera: apoidea) visitation to cantaloupe cucumis melo (cucurvitaceae) flowers in panama].flower visits by bees were observed in a melon cultivated field of san lorenzo district, in chiriqui, panama, from january 6 to february 19, 2002, from 6:30 am to 4:30 pm we recorded the duration of each foraging event and the type of resource collected. flower visits were mostly for nectar collection (approximately 75 %). pollen foraging was concentrated in the first hours of the morning and ended by 11:00 am the mean collection time was similar for both food resources, but was different betwee ...200719069779
an alternative sampling technique for cucumber beetles (coleoptera: chrysomelidae) and diurnal beetle activity on muskmelon.field studies were conducted to investigate the effectiveness of yellow sticky traps as an alternative sampling technique for striped, acalymma vittatum (f.), and spotted, diabrotica undecimpunctata howardi barber, cucumber beetles (coleoptera: chrysomelidae) and the diurnal beetle activity on muskmelon, cucumis melo l., near vincennes, in, in 2003 and 2004. the experimental design included six replications of seven 20-m-long rows each of muskmelon with 1.5 m between rows. on each sampling date, ...200717598544
[effects of air temperature, solar radiation and soil water on dry matter accumulation and allocation of greenhouse muskmelon seedlings and related simulation models].with different sowing dates and irrigation upper limits, the effects of air temperature, solar radiation and soil water on the dry matter accumulation and allocation of greenhouse muskmelon seedlings were studied, with related simulation models established. the results showed that the dry matter accumulation and allocation of the seedlings had correlations with the changes of effective accumulative temperature, accumulative solar radiation, and irrigation upper limits at different seasons in a y ...200718333445
[effects of moisture and light intensity on ecophysiological characteristics of muskmelon seedlings].the muskmelon variety 'xiholuotuo' was used as a test material in order to study the combined effects of substrate moisture and light intensity on growth, development and ecophysiological characteristics of muskmelon seedings in greenhouse. the results showed that the different substrate moisture and light intensity remarkably influenced the growth and development of muskmelon seedlings. with reduction of substrate moisture, chl a, chl b and chl a + b increased, while relative water content (rwc ...200718260450
dielectric properties of honeydew melons and correlation with quality.dielectric properties of three honeydew melon cultivars, grown and harvested to provide a range of maturities, were measured with an open-ended coaxial-line probe and impedance analyzer over the frequency range from 10 mhz to 1.8 ghz. probe measurements were made on the external surface of the melons and also on tissue samples from the edible internal tissue. moisture content and soluble solids content (ssc) were measured for internal tissue samples, and ssc (sweetness) was used as the quality f ...200718161422
[effects of potassium level on photosynthetic characteristics and chloroplast submicroscopic structure of muskmelon leaves].with soilless culture in greenhouse, this paper studied the effects of three potassium (k) levels (120, 240, and 360 mg x l(-1)) on the photosynthetic characteristics and chloroplast submicroscopic structure of muskmelon cv. 'tiantian no. 1' leaves. the results showed that at low (120 mg x l(-1)) and high (360 mg x l(-1)) k levels, the net photosynthetic rate (pn) decreased, chloroplast lamellar structure disordered, and lamella number decreased. no significant effects were observed on co2 compe ...200717650859
determining nitrogen requirements of aceros and buceros hornbills.over 2 months, seven feeding trials were conducted at st. catherines island, ga, to quantify protein intake and utilization in captive mature nonreproducing aceros (n=3 spp.) and buceros (n=2 spp.) hornbills were fed homogeneous isocaloric diets. a mixture of bird of paradise pellets, grapes, and raisins was offered to the birds as grape-sized balls, supplemented with diced cantaloupe melon to maintain hydration. to vary the protein level (range 10.8-22.6%) within the diets, different amounts of ...200819360624
the molecular and biochemical basis for varietal variation in sesquiterpene content in melon (cucumis melo l.) rinds.a combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. sesquiterpenes were generally absent in non-climacteric varieties. two climacteric melon varieties, the green-fleshed 'noy yizre'el', and the orange-fleshed 'dulce' were further examined. in 'noy yizre ...200818264780
antisense suppression of an acid invertase gene (mai1) in muskmelon alters plant growth and fruit development.to unravel the roles of soluble acid invertase in muskmelon (cucumis melo l.), its activity in transgenic muskmelon plants was reduced by an antisense approach. for this purpose, a 1038 bp cdna fragment of muskmelon soluble acid invertase was expressed in antisense orientation behind the 35s promoter of the cauliflower mosaic virus. the phenotype of the antisense plants clearly differed from that of control plants. the transgenic plant leaves were markedly smaller, and the stems were obviously t ...200818641398
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