Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| intestinal microflora in rats: isolation and characterization of strictly anaerobic bacteria requiring long-chain fatty acids. | three strains of strictly anaerobic bacteria, isolated from the cecal contents of rats, have strict requirements for long-chain fatty acids. the effect of exogenous fatty acids on the growth and fatty acid composition of the bacteria was examined. biohydrogenation of linoleic acid into octadecenoic acid was observed. these observations suggest that long-chain fatty acids in the intestine are factors in controlling the localization and the population levels of indigenous bacteria in vivo in rats. | 1976 | 1267446 |
| structural studies of the polysaccharide antigen of eubacterium saburreum, strain 49. | the polysaccharide antigen produced by eubacterium saburreum, strain l 49, is composed of d-glycero-d-galacto-heptose and a new sugar, tentatively identified as 6-deoxy-d-altro-heptose. it contains chains of alternating (1 leads to 3)- and (1 leads to 6)- linked beta-d-glycero-d-galacto-heptopyranosyl residues, the latter being substituted with 6-deoxy-alpha-heptofuranosyl groups at o-3. the polysaccharide further contains 0-acetyl groups, linked to o-7 of part of the heptosyl residues and to o- ... | 1976 | 1268882 |
| [antibacterial and bactericidal activity of tinidazole against anaerobic bacteria comparing with metronidazole (author's transl)]. | antibacterial activity of tinidazole (1-2-(ethylsulfonyl)-2-methyl-5-nitroimidazole) against anaerobic bacteria including peptococcus, peptostreptococcus, eubacterium, propionibacterium, bacteroides and fusobacterium was studied by agar dilution method comparing with metronidazole. in addition to this work, bactericidal effect of tinidazole and metronidazole against p. prevotii, b. fragilis ss. fragilis and f. varium was examined by quantitative culture method after incubation in gam broth conta ... | 1976 | 1271586 |
| bile acid biotransformation rates of selected gram-positive and gram-negative intestinal anaerobic bacteria. | 1976 | 1275920 | |
| treatment of anaerobic pulmonary infections; carbenicillin compared to clindamycin and gentamicin. | twenty-three patients with anaerobic infections of the lung were treated with either two antibiotics, clindamycin and gentamicin (11 patients) or with a single antibiotic, carbenicillin (12 patients). cultures were obtained prior to therapy, either by transtracheal needle aspiration (17 patients) or thoracocentesis (six patients). anaerobic bacteria were found in all. fifteen patients had aerobic and facultative bacteria in addition. the anerobic isolates were peptostreptococci (12), peptococci ... | 1976 | 1277892 |
| arginine, a growth-limiting factor for eubacterium lentum. | eubacterium lentum is a gram-positive, asaccharolytic, obligately anaerobic bacillus, which grows to a low turbidity (absorbancy at 650 nm = 0.05 to 0.1) in peptone-based medium. the addition of substrate amounts of arginine or citrulline dramatically increased growth (absorbancy at 650 nm =1.4). the presence of an arginine dihydrolase pathway was confirmed by measurement of the necessary enzymes and demonstration of the intermediate compounds. the production of adenosine 5'-triphosphate from th ... | 1976 | 182668 |
| aldolases of the lactic acid bacteria. demonstration of immunological relationships among eight genera of gram positive bacteria using an anti-pediococcal aldolase serum. | reciprocal qualitative and quantitative immunological experiments employing an anti-pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the streptococcus faecalis fructose diphosphate aldolase. the extent of immunological relatedness observed between the lactobacillus and pediococcus aldolases was markedly gses indicating that the pediococci share closer phylogenetic ties with th ... | 1976 | 65160 |
| faecal steroids and bacteria and large bowel cancer in hong kong by socio-economic groups. | in a study of three socio-economic groups in hong kong, the high income group had a high faecal concentration of bile acids, especially the dihydroxy bile acids, compared to the low income group. the faecal bile acids were also more highly degraded. the faecal flora contained more bacteroides and fewer eubacteria. very few of the clostridia able to dehydrogenate the steroid nucleus were isolated. an epidemiological study based on street blocks indicated that the high income group also have a hig ... | 1976 | 962996 |
| cytochrome spectrum of an obligate anaerobe, eubacterium lentum. | an obligately anaerobic bacterium, eubacterium lentum, was shown to contain cytochromes a, b, and c and a carbon monoxide-binding pigment. extracts of cells grown with hemin gave a typical absorption spectrum for cytochrome c with maxima at 424, 525, and 553 nm. extracts from cells grown in the absence of hemin also had an absorption peak corresponding to cytochrome b (562 nm) in their reduced versus oxidized spectrum. extraction of hemes and formation of pyridine hemochromes allowed quantitatio ... | 1976 | 176143 |
| evaluation of enrichment, storage, and age of blood agar medium in relation to its ability to support growth of anaerobic bacteria. | by measuring the colony size of a variety of anaerobic bacteria isolated from clinical specimens, an evaluation was made of the benefits derived from the addition of several enrichments to blood agar medium commonly used for the growth of anaerobes. similar methods were used to study the effects of various storage conditions and age of the medium. the results were compared with those obtained on freshly prepared and enriched blood agar plates as well as commercially available blood agar plates. ... | 1976 | 11226 |
| growth patterns of a wide spectrum of organisms encountered in clinical blood cultures using both hypertonic and isotonic media. | in an effort to determine how long a wide spectrum of organisms will survive in either an isotonic or a hypertonic blood culture system, all clinical blood culture flasks were subcultured on a daily basis for the first seven days of incubation and again on the fourteenth day. this subculture included all those flasks found to harbor organisms on previous subculture. organisms such as members of the enterobacteriaeceae and pseudomonadaceae, as well as staphylococcus aureus, bacteroides spp., euba ... | 1976 | 16535815 |
| effect of a high-beef diet on the fecal bacterial flora of humans. | ten human volunteers completed a 4-month diet series consisting of 1 month each of a control diet, a meatless diet, a high-beef diet, and the same control diet. fat and fiber contents were essentially the same in all four diets, but protein content was doubled during the high-beef diet. during the 4th week on each diet, three stool specimens collected from each volunteer were analyzed for chemical composition and content of facultative, aerobic, and anaerobic bacteria. the bacteriological data a ... | 1977 | 832279 |
| composition and antigenic properties of a surface polysaccharide isolated from eubacterium saburreum, strain l452. | 1977 | 65901 | |
| current aspects of gas gangrene, apropos of 47 cases collected over a 3-year period (1974-1976). | on the basis of 47 cases of gas gangrene collected over the three year period between 1974 and 1976, the authors review the circumstances surrounding its development, the clinical features and the prognosis of the disorder which remains grave despite a well-defined therapeutic protocol combining surgery, antibiotics and hyperbaric oxygen. there would appear to be a real resurgence of the disease at the present time. post-traumatic and surgical aetiologies predominate, giving rise to two types of ... | 1977 | 23023 |
| [fecal flora of man. v. communication: the fluctuation of the fecal flora of the healthy adult (author's transl)]. | seven fecal specimens from each of healthy persons, aged 25 to 42 years, during the period of 2 months were analyzed to obtain the knowledge on the day-to-day variation in the same person and the person-to-person variation. irrespective of individuals, bacteroidaceae, catenabacteria (eubacteria and strict anaerobic lactobacilli), peptostreptococci (anaerobic gram positive cocci) and bifidobacteria constituted the predominant flora. spirillaceae, megasphaerae and clostridia (except cl. perfringen ... | 1977 | 196456 |
| nad-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from eubacterium lentum atcc no. 25559. | eubacterium lentum (atcc no. 25559) was shown to contain 3alpha-and 12alpha-hydroxysteroid dehydrogenases both of which were nad-dependent and active against conjugated and unconjugated bile salts. in addition, the 3alpha-hydroxysteroid dehydrogenase was active against members of the androstan series containing a 3alpha-hydroxyl group regardless of the stereo-orientation of the 5-h-. no measurable activity against 7alpha-, 7beta-, 11beta-, or 17beta-hydroxyl groups was demonstrated. the growth o ... | 1977 | 201289 |
| isolation and characterization of human fecal bacteria capable of 21-dehydroxylating corticoids. | it has been known for a decade that human intestinal flora include organisms capable of 21-dehydroxylating corticoids. yet the identity of the organisms synthesizing 21-dehydroxylase has remained unknown. using diluted human feces, we determined the prevalence of colonies of 21-dehydroxylating organisms on a variety of media. isolation from the medium of colonies with the highest prevalence yielded an obligate anaerobe capable of 21-dehydroxylating deoxycorticosterone and tetrahydrodeoxycorticos ... | 1977 | 303887 |
| [polymicrobial septicemia caused by strictly anaerobic bacteria]. | 1977 | 322091 | |
| superoxide dismutase in anaerobic bacteria of clinical significance. | twenty-two anaerobic bacteria isolated from infected sites and normal fecal flora were assayed for superoxide dismutase (sod). the organisms were also classified according to their oxygen tolerance into aerotolerant, intermediate, and extremely oxygen-sensitive groups. there was a correlation between the enzyme level and the oxygen tolerance, in that the aerotolerant and intermediate organisms had sod, whereas the extremely oxygen-sensitive isolates had low or undetectable enzyme. among the oxyg ... | 1977 | 326669 |
| cholesterol-reducing bacterium from human feces. | an anaerobic, gram-positive diplobacillus that reduces cholesterol to coprostanol was isolated from human feces and rat cecal contents. the isolates closely resemble a cholesterol-reducing organism isolated by eyssen et al. (h. eyssen et al., eur. j. biochem. 36:412-421, 1973) from a rat's cecum. these organisms would not form colonies and were isolated and cultivated in an anaerobic medium containing homogenized pork brains (naturally high in cholesterol). these organisms require free or esteri ... | 1977 | 335969 |
| fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon. | a total of 154 strains from 22 species of bifidobacterium, peptostreptococcus, lactobacillus, ruminococcus, coprococcus, eubacterium, and fusobacterium, which are present in high concentrations in the human colon, were surveyed for their ability to ferment 21 different complex carbohydrates. plant polysaccharides, including amylose, amylopectin, pectin, polygalacturonate, xylan, laminarin, guar gum, locust bean gum, gum ghatti, gum arabic, and gum tragacanth, were fermented by some strains from ... | 1977 | 563214 |
| aanerobic isolates in hemovac lines. | seventeen of 68 patients (25 percent) who required the use of hemovac drainage system after major head and neck procedures had positive anaerobic islates. co-isolation of aerobic (94 percent) and other anaerobic (29 percent) microorganisms are common. there was an increased rate of psoitive anaerobic islates with procedures requiring exposure of mucosa. radiotherapy, except in doses greater than 6,800 rads, did not affect the culture rate. eight of 17 patients (47 percent) with positive anaerobi ... | 1977 | 831050 |
| isolation and identification of fecal bacteria from adult swine. | an examination of the fecal microflora of adult swine was made with regard to the efficiency of several roll tube media in enumeration and recovery of anaerobes, the effects of medium constituents on recovery, and the isolation and identification of the predominant kinds of bacteria. total number of organisms by microscopic bacterial counts varied among fecal samples from 4.48 x 10(10) to 7.40 x 10(10) bacteria/g (wet weight). comparison of different nonselective roll tube media indicated that a ... | 1977 | 836025 |
| quantitative bacteriology of the vaginal flora. | quantitative bacteriology was performed on vaginal secretions from healthy adult women. the analysis included a single sample from 17 college students and 35 samples from five volunteers collected at intervals of three to five days throughout the menstrual cycle. mean concentrations in all 52 specimens were 10(8.1) aerobic bacteria/g and 10(9.1) anaerobic bacteria/g. the rank of predominant organisms, according to rates of recovery in concentrations of greater than 10(5) colony-forming units/g, ... | 1977 | 894079 |
| structural studies of the polysaccharide antigen of eubacterium soburreum, strain l. 452. | 1977 | 912694 | |
| fecal microbial flora in seventh day adventist populations and control subjects. | a comparison of 13 vegetarian seventh day adventists with 14 nonvegetarian adventists revealed relatively few statistically significant differences in fecal flora. a separate study involved a comparison of vegetarian adventists (49 subjects), nonvegetarian adventists (45), and non-adventists on a conventional american diet (31) re: the incidence of the c. paraputrificum group in the fecal flora. the adventist groups had significantly fewer c. septicum and c. tertium isolates than the non-adventi ... | 1977 | 920638 |
| effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria. | the inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. the elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were hg2+ (20 microgram/ml), cu2+ (21 microgram/ml), cr6+ (70 microgram/ml), se4+ (73 microgram/ml), ni2+ (160 microgram/ml), cd2+ (175 microgram/ml), as3+ (304 microgram/ml) and as5+ (1610 microgram/ml). the elements tested t ... | 1978 | 565671 |
| nonsporing, anaerobic, gram-positive rods in saliva and the gingival crevice of humans. | quantitative and qualitative examination of anaerobically isolated flora of the gingival crevice and saliva was carried out. it was found that half the organisms were anaerobes and that there were twice as many gram-positive organisms as there were gram-negative ones. rods were predominant in the gingival crevice (60.5%) and cocci in saliva (69.1%). of the total organisms, nonsporing, gram-positive anaerobic rods accounted for 24% in the gingival crevice and 9.7% in saliva. these organisms were ... | 1978 | 646354 |
| bacteria isolated from the duodenum, ileum, and cecum of young chicks. | facultatively anaerobic and strictly anaerobic bacteria colonizing the intestinal tracts of 14-day-old chicks fed a corn-based diet were enumerated, isolated, and identified. colony counts from anaerobic roll tubes (rumen fluid medium) or aerobic plates (brain heart infusion agar) recovered from homogenates of the duodenum, upper and lower ileum, and cecum varied appreciably among samples from individual birds. anaerobic and aerobic counts from the duodenum and ileum were similar. anaerobic coun ... | 1978 | 646359 |
| methanogenesis from acetate: a nonmethanogenic bacterium from an anaerobic acetate enrichment. | a methanogenic acetate enrichment was initiated by inoculation of an acetate-mineral salts medium with domestic anaerobic digestor sludge and maintained by weekly transfer for 2 years. the enrichment culture contained a methanosarcina and several obligately anaerobic nonmethanogenic bacteria. these latter organisms formed varying degrees of association with the methanosarcina, ranging from the nutritionally fastidious gram-negative rod called the satellite bacterium to the nutritionally nonfasti ... | 1978 | 677881 |
| bacteremia due to bifidobacterium, eubacterium or lactobacillus; twenty-one cases and review of the literature. | twenty-one cases of bacteremia due to bifidobacterium, eubacterium and lactobacillus are described. transient bacteremic episodes with these organisms may follow trauma to the mouth, intestine, or vagina. the majority of the patients were female and most had an underlying condition that may have predisposed to bacteremia. ten of the patients died despite antibiotic treatment. | 1978 | 749356 |
| antagonism among the normal anaerobic bacteria of the mouse gastrointestinal tract determined by immunofluorescence. | strictly anaerobic bacteroides sp., eubacterium sp., and fusobacterium sp. were isolated from the cecum of a conventional mouse. an immunofluorescent method utilizing rabbit antisera specific for each of these three strains was developed to determine their population levels in the gastrointestinal tracts of gnotobiotic mice. population levels of these anaerobes in groups of gnotobiotic mice colonized with either bacteroides, eubacterium, or fusobacterium were compared with those of gnotobiotes c ... | 1978 | 354525 |
| a review. lessons from an animal model of intra-abdominal sepsis. | intra-abdominal sepsis that involves multiple aerobic and anaerobic bacteria derived from the colonic flora was studied in wistar rats to determine the relative roles of various microbial species. the rats challenged with pooled colonic contents showed a biphasic disease. initially, there was acute peritonitis, escherichia coli bacteremia, and high mortality. in rats that survived this acute peritonitis stage, intra-abdominal abscesses developed, and anaerobic bacteria were the preponderant orga ... | 1978 | 354591 |
| antimicrobial effect of amniotic fluid. | the antimicrobial effect of amniotic fluid (af) obtained during the first (af1) and second (af2) trimesters was compared with the third (af3) against anaerobic bacteria, such as bacteroides fragilis ss. fragilis (6 strains), eubacterium lentum (3 strains), and peptostreptococcus anaerobius (4 strains). escherichia coli (5 strains) served as a positive control. af1 supported the growth of all 4 anaerobes (except b fragilis for 4 hours) for the entire 24-hour period tested. af2 supported the growt ... | 1978 | 355963 |
| increase in the population of duodenal immunoglobulin a plasmocytes in axenic mice associated with different living or dead bacterial strains of intestinal origin. | various bacterial strains were tested for their ability to stimulate immunoglobulin a (iga) plasmocytes to populate the duodenal lamina propria in axenic mice. the mice were associated with the strains for at least 4 weeks. the strains inhabiting the conventional mouse intestine and belonging to the genera lactobacillus, streptococcus, eubacterium, actinobacillus, micrococcus, corynebacterium, and clostridium (including the extremely oxygen-sensitive ones) are only slightly or nonimmunogenic, wh ... | 1978 | 357289 |
| quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms. | the ability of three different commercial blood culture media--brain heart infusion broth (pfizer), thiol broth (difco), and prs-peptone broth (becton, dickinson & co.)--to support the growth of five different anaerobes is described. inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. in addition, each medium was evaluated for its ability to neutralize the antibact ... | 1978 | 24059 |
| 21-dehydroxylation of corticoids by anaerobic bacteria isolated from human fecal flora. | 1978 | 28451 | |
| anaerobic fecal bacteria of the baboon. | the predominant bacterial genera of baboon feces were enumerated and identified by established procedures. the predominant genera isolated were lactobacillus, eubacterium, streptococcus, and bacteroides. | 1978 | 103499 |
| a polysaccharide antigen from the gram-positive organism eubacterium saburreum containing dideoxyhexose as the immunodominant sugar. | a highly active surface antigen, reacting by precipitation and complement fixation, has been isolated from eubacterium saburreum strain l32. the antigen is a polysaccharide polymer built up of galactose, ribose and dideoxyhexose. the dideoxyhexose is the immunodominant sugar. | 1978 | 670929 |
| the number, physical organization and transcription of ribosomal rna cistrons in an archaebacterium: halobacterium halobium. | because it is now clear that archaebacteria may be as distinct from eubacteria as either group is from eukaryotic cells, and because a specifically archaebacterial ancestry has been proposed for the nuclear-cytoplasmic component of eukaryotic cells, we undertook to characterize, for the first time, the ribosomal rna cistrons of an archaebacterium (halobacterium halobium). we found these cistrons to be physically linked in the order 16s-23s-5s, and obtained evidence that they are also transcribed ... | 1979 | 514815 |
| reduction of aldosterone by anaerobic bacteria: origin of urinary 21-deoxy metabolites in man. | 1979 | 502556 | |
| characterization of a c21 neutral steroid hormone transforming enzyme, 21-dehydroxylase, in crude cell extracts of eubacterium lentum. | a strain of the obligate anaerobe, eubacterium lentum, isolated from human feces, catalyzes the 21-dehydroxylation of 11-deoxycorticosterone to progesterone. a quantitative radiochromatographic assay was developed to measure 21-dehydroxylase activity in cell extracts. maximum enzyme activity in cell extracts required both a reduced pyridine nucleotide and an oxidized flavin coenzyme. however, photochemically reduced flavin (fmnh2) could replace the requirement for nad(p)h plus oxidized flavin. n ... | 1979 | 38850 |
| the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy. | quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ... | 1979 | 118996 |
| [clinical experience of cefoxitin in the field of obstetrics and gynecology (author's transl)]. | cefoxitin was given to the 7 patients of infections in the field of obstetrics and gynecology, and the following results were obtained: 1) the clinical response was excellent in 2 patients, good in 4 and poor in 1 patient with the efficacy rate of 85.7%. out of the 4 patients resistant to the previous therapy with other antibiotics, 3 patients responded to cefoxitin, and all the 3 patients of anaerobic infections responded satisfactorily to cefoxitin. 2) microorganisms isolated were 2 strains ea ... | 1979 | 160956 |
| identification of bacteroides fragilis by indirect immunofluorescence. | rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ... | 1979 | 94651 |
| urease-producing species of intestinal anaerobes and their activities. | urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ... | 1979 | 36839 |
| bile salt 3 alpha- and 12 alpha-hydroxysteroid dehydrogenases from eubacterium lentum and related organisms. | thirty-two strains of eubacterium lentum and phenotypically similar anaerobic gram-positive bacilli were screened for intracellular bile salt 3alpha- and 12alpha-hydroxysteroid dehydrogenase (hsdhase) activities. these organisms were categorized into four groups: (a) those containing 12alpha-hsdhase only (10 strains), (b) those containing 3alpha- and 12alpha-hsdhase (13 strains), (c) those containing 3alpha-hsdhase only (2 strains), and (d) those devoid of any measurable hsdhase activity (7 stra ... | 1979 | 39496 |
| new markers for eubacterium lentum. | of 37 strains of eubacterium lentum and phenotypically similar organisms, 26 (70%) synthesized a corticoid 21-dehydroxylase and/or a 3 alpha-hydroxysteroid dehydrogenase. it appeared that the corticoid 3 alpha-hydroxysteroid dehydrogenase was identical to the bile acid 3 alpha-hydroxysteroid dehydrogenase. steroid-metabolizing enzymes were found both in e. lentum and in phenotypically similar organisms. e. lentum is characterized by nitrate reduction and enhanced growth in the presence of argini ... | 1979 | 314778 |
| susceptibility of new zealand isolates to anaerobic bacteria to tinidazole. | minimum inhibitory and minimum bactericidal concentrations (mic, mbc) of tinidazole for 80 new zealand isolates of anaerobic bacteria were determined. growth of 95 percent of the isolates was inhibited by 4 micrograms/ml or less of tinidazole. mbc values were the same as, or one dilution higher than, the mic. | 1979 | 290923 |
| immuno-electron microscopic localization of a choriogonadotropin-like antigen in cancer-associated bacteria. | the presence of choriogonadotropin-like material and its intimate association with the membranes of the wall of bacteria isolated from cancer patients, has been demonstrated by immuno-electron microscopy utilizing the indirect peroxidase-antiperoxidase-labeled antibody technique. the bacteria were an escherichia coli strain isolated from a carcinoma of colon, and atcc 25559 strain of eubacterium lentum, an anaerobic microorganism originally isolated from a rectal tumor. | 1979 | 384308 |
| the prevalence of anaerobic bacteria in suprapubic bladder aspirates obtained from pregnant women. | the incidence of asymptomatic bacteriuria due to aerobic, facultative anaerobic and strictly anaerobic bacteria during pregnancy was determined in 185 patients admitted for complications of pregnancy, using suprapubic bladder aspiration. anaerobic bacterial counts of 103 per ml urine or higher were detected in 6.4 per cent of the specimens. the incidence of anaerobic bacteriuria seems comparable to that of bacteriuria due to aerobic and facultative anaerobic micro organisms. in our group of pati ... | 1979 | 388942 |
| skin and soft tissue polymicrobial infections from intravenous abuse of drugs. | skin and soft tissue infections were studied in 21 seriously ill narcotic addicts who had been admitted to hospital. subcutaneous abscesses were present in 14 patients; cellulitis was noted in 3, pyomyositis in 2 and necrotizing fasciitis in 2. in four patients there was septicemia. infections in 14 patients (66.6 percent) were associated with anaerobic bacteria, which were the exclusive isolates in 6 patients. in seven patients (33.3 percent) isolates were exclusively aerobic bacteria and in ei ... | 1979 | 425502 |
| chemical structure of the polysaccharide antigen of eubacterium saburreum, strain o2. | the polysaccharide antigen produced by eubacterium saburreum, strain o2, is composed of (1 leads to 6)-linked beta-d-glycero-d-galacto-heptopyranosyl residues, all of which are substituted with 6-deoxy-alpha-d-altro-heptofuranosyl groups at o-3. | 1979 | 427834 |
| bacterial metabolism of corticoids with particular reference to the 21-dehydroxylation. | clostridium paraputrificum, an obligate anaerobe recovered from human feces, reduces the alpha,beta-unsaturated carbonyl of deoxycorticosterone, corticosterone, cortisone, and cortisol. the same steroids are 21-dehydroxylated by culture 116, recently isolated from human feces, and by a closely related organism, eubacterium lentum. the 21-dehydroxylase has no effect on hydroxyl groups at carbon atoms 11 and 17. | 1979 | 429305 |
| antimicrobial activity of antituberculosis agents against anaerobic bacteria. | anaerobic infections may coexist with tuberculosis, and can be mistaken for one another. the effect of therapy with antituberculosis chemotherapeutic agents against anaerobic bacteria (with the exception of rifampin) is unknown. we therefore examined the in vitro efficacy of certain commonly used antituberculosis agents (rifampin, isoniazid, and ethambutol) against 370 strains of anaerobic bacteria, including 86 isolates of bacteroides fragilis. rifampin at a concentration of 2 microgram/ml inhi ... | 1979 | 436485 |
| fecal bacteria in south african rural blacks and other population groups. | quantitative studies were performed on fecal flora of three population groups consuming different diets. twenty rural black south africans and 22 japanese, representing groups at low risk for carcinoma of the colon, were compared with 41 north americans from a high-risk population. specimens taken immediately after defecation were mixed and processed under anaerobic conditions. after the initial incubation, roll tubes were shipped to the united states for final identification. bacterioides and b ... | 1979 | 447500 |
| [short-term oral chemoprophylaxis before intestine surgery. quantitative determination of bacteria and fungi in stool specimens (author's transl)]. | the methods of quantitative analysis of aerobe and anaerobe microbes and fungi stool specimens are described. the results of the studies in health people are compared to the results in patients undergoing surgical treatment of intestinal tract. a group of these patients received neomycin and bacitracin orally as short-time chemoprophylaxis to diminish possible woundinfection and/or sepsis. after oral medication germs as bifidobacterium, bacteroides and clostridium (not cl. perfringens) are reduc ... | 1979 | 452770 |
| choriogonadotropin-like antigen in an anaerobic bacterium, eubacterium lentum, isolated from a rectal tumor. | using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive material in a strain of eubacterium lenthum that was originally isolated from a rectal tumor. in contrast, both immunohistochemical reactions were negative when applied to a strain of co ... | 1979 | 468380 |
| antibiotic treatment of splenic abscess. | 1979 | 475190 | |
| chlortetracycline and sulfonamide resistance of fecal bacteria in swine receiving medicated feed. | the fecal bacterial flora of swine receiving a ration supplemented with chlortetracycline, sulfamethazine, and penicillin was tested for resistance to chlortetracycline and sulfamethazine using anaerobic techniques and medium m-10. approximately 15.5 and 1.4% of the flora grew in the presence of 25 and 100 microgram of tetracycline/ml, respectively. higher numbers of bacteria grew in the presence of similar concentrations of sulfamethazine. thirty-five chlortetracycline-resistant isolates were t ... | 1979 | 476553 |
| plasmenylethanolamine: growth factor for cholesterol-reducing eubacterium. | a plasmalogen, plasmenylethanolamine, is required for in vitro growth of strains of eubacterium which convert cholesterol to coprostanol. plasmenylethanolamine was isolated from calf brain by selective saponification of lipid fractions separated by thin-layer or column chromatography. cholesterol-containing thioglycolate broth plus purified plasmenylethanolamine or its 2-lyso derivative supported growth of eubacterium atcc 21408 and a cholesterol-reducing eubacterium isolated from baboon feces. ... | 1979 | 479108 |
| aerobic and anaerobic bacterial flora in semen from fertile and infertile groups of men. | semen samples were collected by masturbation under asceptic conditions from men who had sired children within the past 6 mo (group a) and asymptomatic men attending an infertility clinic who had not sired children and whose wives were asymptomatic for infertility (group b). these 109 semen samples were analyzed and cultured for isolation of aerobic and anerobic organisms. overall, 68% of the specimens had positive bacterial cultures: 54% of the samples from group a were positive and 73% from gro ... | 1979 | 485650 |
| recovery of anaerobic bacteria from pediatric patients. a one-year experience. | during 1975, 115 speciments from the soft tissues, body fluids, and purulent sites in 105 pediatric ward and nursery patients were submitted to the clinical laboratory for anaerobic study, and 75% of the cultures were positive. in most instance specimens were collected in anaerobic transport tubes containing peptone yeast extract and glucose. in the laboratory, samples were processed by the anaerobic holding jar method. nineteen different anaerobic organisms were isolated; the average number of ... | 1979 | 495592 |
| comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline. | the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ... | 1979 | 543353 |
| the effect of l-alpha-amino-n-butyric acid on growth and production of extracellular isoleucine and valine by eubacterium ruminantium and a related rumen isolate. | two anaerobic rumen bacteria, eubacterium ruminantium and a closely related isolate, were studied to determine the effect of the valine antimetabolite alpha-aminobutyric acid on growth and production of extracellular isoleucine and valine in an amino acid free medium. in the absence of alpha-aminobutyrate, these organisms actively excreted valine during growth (90-195 microgram/ml) but only accumulated limited concentrations of isoleucine (3-7 microgram/ml) in the culture broth. growth of both o ... | 1979 | 575311 |
| changes in lactate-producing and lactate-utilizing bacteria in relation to ph in the rumen of sheep during stepwise adaptation to a high-concentrate diet. | changes in the numbers and types of lactate-producing and lactate-utilizing bacteria in the rumen of sheep were followed during stepwise adaptation from a low- to a high-concentrate diet. the mean numbers of bacteria increased after each change in diet when increasing amounts of maize grain were substituted for maize stover. a surge in number of amylolytic bacteria always preceded an increase in lactate-utilizing bacteria, and with the final diet containing 71% grain and molasses the two groups ... | 1979 | 16345431 |
| comparison of moxalactam (ly127935) and cefotaxime against anaerobic bacteria. | the in vitro activities of moxalactam (ly127935 [6059s]) and cefotaxime were compared with those of cefoxitin, cefamandole, cefuroxime, carbenicillin, and penicillin by agar dilution susceptibility testing of a variety of anaerobic bacteria. moxalactam proved to be the most active agent tested against bacteroides fragilis and other species of the b. fragilis group. moxalactam and cefotaxime showed activity similar to the other drugs against the remaining species of bacteroides, fusobacterium, ac ... | 1980 | 6249198 |
| inhibitory effect of some intestinal bacteria on liver tumorigenesis in gnotobiotic c3h/he male mice. | liver tumorigenesis in gnotobiotic c3h/he male mice was markedly promoted by association with a bacterial combination of escherichia coli, streptococcus faecalis, and clostridium paraputrificum. this study demonstrated that this promoting effect was suppressed by addition of certain intestinal bacteria, such as bifidobacterium longum, lactobacillus acidophilus, and eubacterium rectale. | 1980 | 6780184 |
| biochemical characterization of cholesterol-reducing eubacterium. | we characterized two isolates of cholesterol-reducing eubacterium by conducting conventional biochemical tests and by testing various sterols and glycerolipids as potential growth factors. in media containing cholesterol and plasmenylethanolamine, the tests for nitrate reduction, indole production, and gelatin and starch hydrolyses were negative, and no acid was produced from any of 22 carbohydrates. both isolates hydrolyzed esculin to esculetin, indicating beta-glycosidase activity. in addition ... | 1980 | 6779702 |
| characterization of a corticosteroid 21-dehydroxylase from the intestinal anaerobic bacterium, eubacterium lentum. | an oxygen-sensitive corticosteroid 21-dehydroxylase has been characterized in cell extracts of eubacterium lentum. the enzyme was highly specific for corticosteroids containing and alpha-ketol structure and required fmnh2 or reduced benzyl viologen for activity. the enzyme used deoxycorticosterone, deoxycortisol, dehydrocorticosterone, and corticosterone as substrates. substrate saturation kinetics using [3h]corticosterone yielded an apparent km of 7.35 microm and a vmax of 15.4 nmol (11 beta-[3 ... | 1980 | 6967506 |
| rapid gas-chromatographic method for identification of metabolic products of anaerobic bacteria. | the volatile fatty acids, alcohols, nonvolatile fatty acids, and ketones produced by anaerobes can be separated and identified by using a single type of gas-chromatographic column. a rapid and simple procedure is described. | 1980 | 6989858 |
| microorganisms associated with abscesses of sheep and goats in the south of iran. | a total of 86 abscesses (45 and 41) abscesses of sheep and goats, respectively) were examined for their causal agents; 44 of these abscesses were located subcutaneously, and the remaining 42 were in lungs, livers, intestines, and udders. a total of 23 different types of microorganisms were isolated from 78 abscesses; bacteria were not detected in the remaining eight abscesses. microorganisms isolated were: species of the genera corynebacterium, staphylococcus, and streptococcus and pasteurella, ... | 1980 | 6996545 |
| spurious hydrogen sulfide production by providencia and escherichia coli species. | hydrogen sulfide production was noted in two escherichia coli strands and one provaidenica alcalifaciens (proteus inconstans a) strain isolated from clinical stool specimens durin the summer of 1979. an investigation into this phenomenon revealed the predence of eubacterium lentum, an anaerobe, growing in synergism with the enterobacteriaceae and producing h2s. the implications of this association are discssed with reference to clinical microbiology laboratory practice. | 1980 | 7000823 |
| [saprophytic and opportunistic non spore-forming anaerobic microflora of the vagina (author's transl)]. | a microbiological survey has been carried out on 179 healthy, child-bearing aged, non-pregnant women, with the aim to evaluate the incidence of anaerobic non-spore forming bacteria in the normal vaginal flora. this group of bacteria has been isolated in 50.3% of women, with a clear prevalence of "anaerobic streptococci " and bacteroides, followed by fusobacterium and veillonella. no propionibacterium, eubacterium or bifidobacterium have been isolated. according to many authors the non-spore form ... | 1980 | 7018415 |
| do anaerobes cause urinary tract infection? | 1980 | 6101823 | |
| neutrophil chemotaxis induced by anaerobic bacteria isolated from necrotic dental pulps. | anaerobic bacteria belonging to the species bacteroides melaninogenicus, bacteroides asaccharolyticus, fusobacterium nucleatum, peptostreptococcus anaerobius and eubacterium alactolyticum, isolated from necrotic dental pulps, were tested for their capacity to induce neutrophil leukocyte chemotaxis. generation of chemotactic factors in serum by whole bacterial cells and chemotaxis induced by bacterial extracts were studied in vitro by the boyden technique. subcutaneously implanted wound chambers ... | 1980 | 6103573 |
| in vitro inhibition of neisseria gonorrhoeae growth by strict anaerobes. | thirty two anaerobic bacterial strains were screened for their in vitro inhibitory activity toward 21 neisseria gonorrhoeae strains, including two penicillin-resistant strains. from this screening, six anaerobic bacteria belonging to the genera bacteroides, peptostreptococcus, and eubacterium were selected for their large antigonococcal spectrum of activity. the inhibitory activity of these six strains appears to be specific to the gonococcus as a variety of anaerobes and aerobes were not genera ... | 1980 | 6105130 |
| the assay on a defined medium of the effects of beta-2-thienylalanine on the growth of anaerobic bacterial isolates from phenylketonuric patients. | faecal samples were taken from three diet-managed phenylketonuric children to determine effects of beta-2-thienylalanine (beta-2-t) on indigenous bacteria. from sample swabs, 127 anaerobes were identified and tested for beta-2-t inhibition on a phenylalanine (phe)-free medium, anaerobe inhibition test (ait) agar. of the isolates, 77.9% grew sufficiently to assay reactions on at least 25% of ait plates. using phe-containing columbia agar, 86.5% of the strains could be assayed. none of 28 bacteroi ... | 1980 | 7189815 |
| pleuropulmonary infection associated with eubacterium brachy, a new species of eubacterium. | a new species of eubacterium was isolated from a case of pleural effusion. a case history and description of the organism are given. | 1980 | 7276145 |
| anaerobic prostatic abscess. | 1980 | 6928548 | |
| a possible role of eubacterium and peptostreptococcus species in the aetiology of crohn's disease. | the faecal flora of patients with crohn's disease has been found to contain higher numbers of anaerobic gram-negative rods and gram-positive coccoid rods than the flora of healthy subjects. in a considerable percentage of sera from patients with crohn's disease, agglutinating antibodies were found against four strains of the coccoid rods, in contrast to sera from patients with ulcerative colitis, other diseases and healthy subjects. in the present study, coccoid strains c18, me46 and me47 were f ... | 1980 | 7235691 |
| utilization of ammonia nitrogen by intestinal bacteria isolated from pigs. | in a medium containing ammonia, proteose peptone, and cysteine as nitrogen sources, 17 of 24 bacteroidaceae strains, 3 of selenomonas strains, 1 of 7 curved rods, 3 of 7 spirochaetaceae strains, 8 of 20 eubacterium strains, 8 of 13 peptococcaceae strains, 3 of 4 clostridium strains, 19 of 20 enterobacteriaceae strains, and 1 of 8 streptococcus strains utilized ammonia nitrogen preferentially to proteose peptone nitrogen. to determine the ability of intestinal microbes to synthesize amino acids f ... | 1980 | 7356320 |
| characterization of a nadh:flavin oxidoreductase induced by cholic acid in a 7 alpha-dehydroxylating intestinal eubacterium species. | a nadh:flavin oxidoreductase was partially purified (seven-fold) from an intestinal eubacterium species v.p.i. 12708 using bio-gel a 0.5-m and deae-cellulose column chromatography. enzyme activity was measured spectrophotometrically at 340 nm under anaerobic conditions. a molecular weight of 260 000 was estimated by gel filtration chromatography. the partially purified enzyme preparation exhibited single displacement kinetics with respect to the substrates nadh and fad. the ph optimum under thes ... | 1980 | 7370273 |
| bile acid induction specificity of 7 alpha-dehydroxylase activity in an intestinal eubacterium species. | the addition of cholic acid to growing cultures of eubacterium species v.p.i. 12708 caused a 25 and 46-fold increase in 7 alpha-dehydroxylation activity using cell extracts or whole cell suspensions, respectively. bile acid conversion rates using either [14c]-cholic acid or [14c]-chenodeoxycholic acid as substrates increased at approximately the same rate when either cholic or chenodeoxycholic acid was added to growing cultures as inducer. the induction of 7 alpha-dehydroxylase activity was high ... | 1980 | 7376208 |
| effect of titanium (iii) citrate as reducing agent on growth of rumen bacteria. | we compared the growth of 10 strains of rumen bacteria in an anaerobic medium reduced with cysteine hydrochloride, dithiothreitol, or titanium (iii) citrate. the redox potential of medium reduced with cysteine hydrochloride was -167.8 mv; with dithiothreitol it was -175.8 mv; and with titanium(iii) citrate it was -302.4 mv at a concentration of 5 x 10(-4) m titanium and -403.9 mv at 2 x 10(-3) m titanium. maximum growth of the strains was generally lower with dithiothreitol or titanium(iii) citr ... | 1980 | 7406484 |
| isolation and identification of 3,6-dideoxy-3-(l-glyceroyl-amino)-d-glucose. a constituent of the antigenic polysaccharide of eubacterium saburreum, strain v5. | 3,6-dideoxy-3-(l-glyceroylamino)-d-glucose has been identified, for the first time, as a sugar component of the antigenic polysaccharide of eubacterium saburreum strain v5, principally by n.m.r. and mass spectrometry. | 1980 | 7407797 |
| biosynthesis of vitamin b12. experiments with the anaerobe eubacterium limosum and some labelled substrates. | 1. the anaerobic microorganism eubacterium limosum (dsm 20 402), grown in the presence of l-[methyl-14c]methionine, produces vitamin b12 not only labeled in the corrin ring, but also in the 5,6-dimethylbenzimidazole moiety. the vitamin b12 obtained in a similar experiment with l-[methyl-13c]methionine showed nine signals in the 13cv nmr spectrum. seven of these signals are due to the seven 'extra' methyl groups of the corrin ring, two signals originate from the two methyl groups of the 5,6-dimet ... | 1980 | 7408871 |
| surface vs core-tonsillar aerobic and anaerobic flora in recurrent tonsillitis. | specimens from both the surface and the core of tonsils from 23 children with recurrent tonsillitis were cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora were obtained from all patients. the predominant anaerobic isolates were bacteroides sp, fusobacterium nucleatum, anaerobic gram-positive cocci, and eubacterium sp. the predominant aerobic isolates were alpha-hemolytic streptococci, staphylococcus aureus, beta-hemolytic streptococci, and haemophilus sp. beta- ... | 1980 | 7411827 |
| structural studies of the eubacterium saburreum strain o 2 antigen. | 1980 | 7417964 | |
| role of alpha-tocopherolquinol in biohydrogenation. | 1980 | 7422159 | |
| isolation and characterization of fecal bacteria capable of 16 alpha-dehydroxylating corticoids. | for more than a decade it has been known that the fecal flora of humans and rats includes organisms capable of 16 alpha-dehydroxylating corticoids, but their identity has remained unknown. to isolate these organisms, mueller-hinton agar plates were seeded with fresh feces from proteus-free rats and incubated anaerobically. on an average, 1 of every 35 colonies consisted of organisms synthesizing 16 alpha-dehydroxylase. isolation of the individual colonies yielded two obligate anerobes, strains 1 ... | 1980 | 7425626 |
| agglutinins to bacteria in crohn's disease. | sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ... | 1980 | 7429299 |
| effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria. | the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ... | 1980 | 7431377 |
| 7 alpha-dehydroxylation of cholic acid by cell extracts of eubacterium species v.p.i. 12708. | nadh:flavin oxidoreductase and 7 alpha-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of eubacterium species v.p.i. 12708. assays of 7 alpha-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of nad+ plus fadh2. the ph optima of 7 alpha-dehydroxylase activity in whole cells and cell extracts were 7.0. the similar induction pattern of these two enzymes and the apparent cofactor requirements f ... | 1980 | 7435421 |
| fermentation of 1,2-o-iso-propylidene-d-glucofuranose ("monoacetone glucose") by anaerobic bacteria. | various species of clostridium, bacteroides, propionibacterium and eubacterium were incubated in a 1% solution of 1,2-o-iso-propylidene-d-glucofuranose in a peptone-yeast-extract (py) medium according to the vpi-technique. the volatile and non volatile acids were investigated by gas-liquid chromatography. all microorganisms showed a pattern of vfa different from that one produced by the glucose containing medium. in most microorganisms the formation of acetic acid was suppressed while the produc ... | 1980 | 7456850 |
| growth of cholesterol-reducing eubacterium on cholesterol-brain agar. | an agar medium containing 5% cholesterol has been developed for the isolation, enumeration, and characterization of cholesterol-reducing strains of eubacterium. | 1980 | 7458310 |
| faecal bacterial flora in cancer of the colon. | selective aerobic and anaerobic plate media were employed to isolate the predominant faecal flora of patients with cancer of the colon (cc), cancer with non-gastrointestinal involvement (ngi), and with non-malignant diseases (n). the cc and n groups did not differ significantly in either total aerobic or anaerobic counts. the cc group did have a significantly lower anaerobic/aerobic ratio compared with the n group (2.42 vs. 2.96, p less than 0.05). this was the result of a greater predominance o ... | 1980 | 7429334 |
| loss of tritium from coprostanone derived from [1,2(n)-3h]cholesterol or [7(n)-3h]cholesterol. | after oral administration of a mixture of [1,2(n)-3h]cholesterol and [4-14c]cholesterol to a baboon, fecal coprostanone had a 46% lower 3h/14c ratio than the dose administered. loss of 3h by enolization of the 3-ketone could account for the decrease in 3h/14c. if [7(n)-3h]cholesterol was administered instead of [1,2(n)-3h]cholesterol a 23% loss of 3h from coprostanone was found. procedures requiring measurement of 3h-coprostanone derived from [1,2(n)-3h]- or [7(n)-3h]cholesterol could be serious ... | 1980 | 6770018 |
| rapid tests for esculin hydrolysis by anaerobic bacteria. | esculin hydrolysis is one of the biochemical tests used in the identification of anaerobic microorganisms. the conventional method by use of growing microbial cells requires 24-48 hours of incubation. on the other hand, growth independent methods like the buffered esculin test, the spot test, and the pathotec strip test utilize the presence of constitutive enzymes and, therefore, yield results in 1-4 hours. a total of 817 anaerobic organisms were used in this study to determine the sensitivity a ... | 1981 | 7044307 |
| [effect of atrazine, linuron and 2, 4-d amine on various biological properties of a soil sample. i - field trial]. | herbicides have a considerable influence on soil microorganisms and soil biochemistry. these influences are likely to be reflected in soil fertility and plant growth. the effects of atrazine, linuron and 2,4-d amine were studied on soil microflora in a field trial with sorghum in río cuarto, argentina. atrazine and linuron were applied before sowing and before emergency, and 2-4,-d amine as post-emergence herbicide. dehydrogenase activity with ttc (tri-cl-phenyl-tetrazolium) as electron acceptor ... | 1981 | 7346888 |