Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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physiology of sporeforming bacteria associated with insects: minimal nutritional requirements for growth, sporulation, and parasporal crystal formation of bacillus thuringiensis. | a defined medium is described in which 18 strains of bacillus thuringiensis representing the 12 established serotypes grow, sporulate, and produce a parasporal crystal. this minimal medium contains glucose and salts supplemented with either aspartate, glutamate, or citrate. these organic acids are required and cannot be replaced by vitamin mixtures or succinate even though succinate is taken up at a rate similar to that of aspartate, glutamate, and citrate. | 1974 | 4844274 |
physiology of sporeforming bacteria associated with insects: radiorespirometric survey of carbohydrate metabolism in the 12 serotypes of bacillus thuringiensis. | radiorespirometry was used to compare the primary pathways of glucose catabolism in 18 strains of bacillus thuringiensis representing the 12 established serotypes. every strain utilizes the embden-meyerhof-parnas pathway almost exclusively; pentose-phosphate pathway participation is minor. the embden-meyerhof-parnas pathway predominates regardless of whether the cells were grown in a minimal medium or one containing yeast extract. the results indicate that the absolute requirement for citrate an ... | 1974 | 4844275 |
sporulation of bacillus thuringiensis without concurrent derepression of the tricarboxylic acid cycle. | bacillus thuringiensis sporulates in a glucose-glutamate medium without concurrent derepression of the tricarboxylic acid cycle. glutamate appears to regulate tricarboxylic acid cycle activity as well as to influence spore heat resistance and production of dipicolinic acid. | 1974 | 4587611 |
heterotrophic nature of the cell-free protein-synthesizing system from the strict chemolithotroph, thiobacillus thiooxidans. | a cell-free protein-synthesizing system prepared from the strict chemolithotroph, thiobacillus thiooxidans, was similar to that of heterotrophs. the poly-u directed system had a temperature optimum of 37 c, but in the presence of spermidine (3 mm) the optimum shifted to 45 c. although growth of the chemolithotroph occurs only in acid conditions, the ph optimum for the cell-free system was ph 7.2. the endogenous-directed activity in the presence or absence of spermidine was maximal at ph 7.8. spe ... | 1974 | 4590488 |
bioassay of a nuclear-polyhedrosis virus and bacillus thuringiensis against the american bollworm, heliothis armigera, in botswana. | 1974 | 4597764 | |
the fine structure of bacillus finitimus and bacillus thuringiensis spores with special reference to the location of crystal antigen. | 1974 | 4615125 | |
structure of two phages of bacillus thuringiensis and b. cereus. | 1974 | 4132606 | |
postflight analysis of bacillus thuringiensis organisms exposed to spaceflight conditions on apollo 16. | 1974 | 4154705 | |
study of the sporulation of bacillus thuringiensis var. thuringiensis. | during the submerged cultivation of bacillus thuringiensis var. thuringiensis in 300- and 3000-liter fermentors, lysis occurred at the end of the exponential phase of growth. new vegetative cells were subsequently formed which usually sporulated. at time of lysis, the amount of soluble sugar was 1-12 g/liter, ph value dropped to 5.3-5.8 from the original ph 6.8 and started to rise after all the cells had lysed. the proteolytic activity was low during the lysis and increased as the sporulation co ... | 1975 | 1076 |
interrelationship between the intestinal microflora of lackey moth, brown-tail moth and the entomopathogenic bacterium bacillus thuringiensis. | the interrelationship between the intestine microflora of lackey moth (malacosoma neustria l.), brown-tail moth (nygmia phaeorrhoea l.) and entomopathogenic bacteria bacillus thuringiensis (berl.), bac. thuringiensis var. galleriea, and bac. thuringiensis kr3 was studied in vitro. various organisms of the intestine microflora of lackey moth display bacteriostatic action towards bac. thuringiensis var. galleriea and bac. thuringiensis kr3 which is not typical of the microflora of brown-tail moth ... | 1975 | 2842 |
production of lecithinase by bacillus thuringiensis. | the activity of lecithaniase was determined in 24 strains of bacillus thuringiensis on four growth media. the highest accumulation of lecithinase was found on the hottinger medium containing 0.5% of glucose and 0.56% of sodium bicarbonate. lecithinase appears at the logarithmic growth phase, and its activity is maximal after 10 hours of growth (at the beginning of the stationary phase). biosynthesis and accumulation of lecithinase occur at ph 6.0 to 9.0. lecithinase was purified by salting out w ... | 1975 | 2844 |
methods for assessing field persistence of bacillus thuringiensis spores. | 1975 | 1090681 | |
temperature-dependent inhibition of polyphenylalanine formation by the exotoxin of bacillus thuringiensis. | 1975 | 1093882 | |
polyamine and magnesium contents and polypeptide synthesis as a function of cell growth. | 1975 | 1096886 | |
dependency of spermidine stimulation of polypeptide synthesis on the uracil content of messenger ribonucleic acid. | 1975 | 1106408 | |
effect of ultraviolet and gamma rays on the activity of delta-endotoxin protein crystals of bacillus thuringiensis. | 1975 | 1112999 | |
lipid metabolism during bacterial growth, sporulation, and germination: kinetics of fatty acid and macromolecular synthesis during spore germination and outgrowth of bacillus thuringiensis. | the timing and kinetics of fatty acid synthesis are delineated for bacillus thuringiensis spore germination and outgrowth by analyzing [u-14c]acetate and [2-3h]glycerol incorporation into chloroform-methanol-extractable and trichloroacetic acid-precipitable lipids. in addition to measurement of pulsed and continuous labeling of fatty acids, monitoring the incorporation of radioactive phenylalanine, thymidine, and uridine from the onset of germination through first cell division provides a profil ... | 1975 | 1116987 |
effect of tree species on the coverage and field persistence of bacillus thuringiensis spores. | 1975 | 1117166 | |
photoprotection against inactivation of bacillus thuringiensis spores by ultraviolet rays. | 1975 | 1117170 | |
enhancement of immune response by the proteinaceous crystal of bacillus thuringiensis var thuringiensis. | 1975 | 1120067 | |
effect of the exotoxin of bacillus thuringiensis on the biosynthesis and maturation of mouse liver nuclear rna. | the effect of the exotoxin of bacillus thuringiensis on the in vivo incorporation of [14-c] orotic acid into mouse liver nuclear rrna and low molecular weight rna was studied. the following results were obtained. 1. the exotoxin does not inhibit the synthesis of 45 s pre-rrna, but causes a breakdown of these molecules. 2. the exotoxin inhibits the conversion of 38 s pre-rrna into 32 s and 21 s. 3. the exotoxin inhibits the labelling of nuclear 5 s rna, whereas the labelling of 4.6 s pre-trna is ... | 1975 | 1125318 |
production of molybdenum-coordinating compound by bacillus thuringiensis. | bacillus thuringiensis (atcc 10792) produces a molybdenum reactive compound (given the trivial name chelin) during growth on iron-deficient medium. this compound accumulates in the culture medium in direct relation to the amount of l-arginine added and reaches a maximum concentration 24 to 48 h after the stationary phase of growth. chelin absorbs light in the ultraviolet region with absorption maxima at 315 and 248 nm and minima at 284 and 240 nm. chelin reacts with na2moo4, but not with mo2o4(h ... | 1975 | 1126915 |
safety of bacillus thuringiensis for earthworms. | 1975 | 1151099 | |
[identification of bacteriophages and study of the properties of phage-resistant mutants of bacillus thuringiensis var. galleriae]. | 1975 | 1156618 | |
evaluation of beta-exotoxin of bacillus thuringiensis berliner for control of flies in chicken manure. | 1975 | 1159722 | |
[larval development of hymenoptera parasites apanteles glomeratus l. and phanerotoma flavitestacea f. in caterpillars infected by bacillus thuringiensis berliner]. | 1975 | 1163945 | |
[pathogenic activity of bacillus thuringiensis on glossina (diptera-muscidae). study on glossina tachinoides in the republic of chad]. | 1975 | 1167198 | |
bacteria as insect pathogens. | 1975 | 1180511 | |
gamma-aminobutyric acid pathway and modified tricarboxylic acid cycle activity during growth and sporulation of bacillus thuringiensis. | enzymatic analyses of bacillus thuringiensis extracts suggest that a modified krebs tricarboxylic acid cycle (without alpha-ketoglutarate dehydrogenase) can operate during sporulation in conjunction with the glyoxylic acid cycle and the gamma-aminobutyric acid pathway. | 1975 | 1180554 |
polyadenylation of rna in vitro in isolated chromatin and nuclei. | poly(a) is added post-transcriptionally to rna transcribed in vitro by endogenous form b dna-dependent rna polymerase bound to the template in isolated nuclei or chromatin. it is also added to processed fragments of the products from alpha-amanitin-resistant rna polymerases a and/or c. the poly(a) segments are of similar size to those found in nuclear rna pulse-labelled in vivo and are added onto the 3' terminus of rna chains (whether pre-existing, completed during the incubation in vitro or cre ... | 1975 | 1183434 |
studies of the infection of the cockroaches (blattella germanica[l.]) with bac. thuringensis. | the results of an experimental infection of the cockroaches blattella germanica (l.) with some trade preparations containing bac. thuringensis spores are presented. biological studies supported by bacteriological and histological examinations of the excised intestines of the above insects have shown that the microorganisms studied are pathogenic for the cockroaches. this effect might be eventually applied as a biological method of eradication of the cockroaches in such environments, where chemic ... | 1975 | 1189795 |
lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched- and normal-chain fatty acids during spore germination and outgrowth of bacillus thuringiensis. | the biosynthesis of individual branched- and normal-chain fatty acids during bacillus thuringiensis spore germination and outgrowth was studied by comparing pulsed and continuous labeling of these fatty acids with [u-14c]acetate. the relative specific activity of each fatty acid varies with time as the cell progresses through outgrowth. however, fatty acid synthesis does occur in two distinct phases. upon germination, acetate is incorporated only into the iso-isomers i-c13, i-c14, and i-c16; no ... | 1975 | 1194236 |
sporeless mutants of bacillus thuringiensis. ii. mutants derived from var. thuringiensis and var. sotto. | three sporeless mutants of bacillus thuringiensis, 2 derived from var. thuringiensis and 1 from var. sotto were selected after mutagenic treatment. they were completely lacking in ability to form spores, yet maintained intact insecticidal activity. | 1975 | 1204774 |
action of bacillus thuringiensis preparation against larch bud moth, zeiraphera diniana (gn.), enhanced by beta-exotoxin and ddt. | addition of either ddt or the supernatant of a centrifuged liquid culture of bacillus thuringiensis, serotype h1, containing beta-exotoxin, enhanced the action of the bacterial preparation thuricide 90 t against larvae of the larch pest zeiraphera diniana, increasing mortality from 53% to more than 80%. since ddt alone produced 57% mortality, its combined action corresponds to independent action. the preparation of beta-exotoxin, on the other hand, had only little effect alone but synergized the ... | 1975 | 1204775 |
separation of spores and parasporal crystals of bacillus thuringiensis in gradients of certain x-ray contrasting agents. | spores and parasporal crystals of bacillus thuringiensis can be separated at moderate centrifugation speeds (10,000 to 12,000 rpm) in gradients of renografin or sodium diatrizoate. | 1975 | 1211935 |
[specific phages used for differentiation of the entomopathogenic bacterium bacillus thuringiensis]. | the sensitivity to specific phages and morphological, physiological, and antigenic properties were compared among several strains of bacillus thuringiensis isolated from insects inhabiting various geographical zones. all 43 cultures assigned to bac. thuringiensis var. sotto and 198 among 170 cultures classed as bac. thuringiensis var. dendrolimus were found to belong to bac. thuringiensis var. dendrolimus. none of these cultures was resistant to its specific phage. the same was true of 22 studie ... | 1975 | 1214610 |
observations on the nature of resistance in spodoptera litura (f.) (noctuidae: lepidoptera) to infection by bacillus thuringiensis berliner. | 1975 | 1218923 | |
[the role of spores and crystals of bacillus thuringiensis berliner in the infection of laspeyresia pomonella l. (tortricidae) (author's transl)]. | susceptibility of laspeyresia pomonella to the crystal toxin of bacillus thuringiensis is demonstrated by forced and free ingestion of purified preparations. the effect of spores alone is weak and cannot be expressed by a relationship between dose and mortality. crystals and spores exhibit better activity when they are associated. | 1975 | 1227376 |
[toxicity of various strains of bacillus thuringiensis isolated from dead larvae of prays olease bernard]. | 1975 | 1234620 | |
[bacillus thuringiensis berliner mutants resistant to various antibiotics and showing alterations of sporulation]. | mutants resistant to oxytetracyclin, erythromycin and neomycin but not to streptomycin, often shown, in the absence of antibiotic, alterations in sporulation: slight or pronounced temperature-sensitive character between 30 and 37 degrees c, slight thermoresistance of refractive spores formed at 30 degrees c, oligosporogenic character at 30 degrees c. | 1975 | 806389 |
an insect toxin from spores of bacillus thuringiensis and bacillus cereus. | spores of bacillus thuringiensis contain a toxin active against lepidopterous larvae. this toxin can be solubilized by extraction with reagents which dissolve the protein crystal of b thuringiensis. it is inactivated by crystal-specific antiserum. spores of bacillus cereus contain a similar toxin although the specific activity is much lower than the spores of b. thuringiensis. the b. cereus toxin contains a single major polypeptidecomponent. toxic activity can be solubliized from spores of both ... | 1975 | 806656 |
lipid metabolism during bacterial growth, sporulation, and germination: an obligate nutritional requirement in bacillus thuringiensis for compounds that stimulate fatty acid synthesis. | the regulation of fatty acid biosynthesis by compounds that are required for growth of bacillus thuringiensis was investigated using an vivo assay developed to measure fatty acid synthesis in germinating spores. a minimal glucose-ammonium-salts medium does not support growth even though previous radiorespirometric studies have shown b. thuringiensis to possess intact tricarboxylic acid and embden-meyerhof-parnas pathways. abundant growth does occur, however, when this medium is supplemented with ... | 1975 | 807563 |
[the effect and fate of bacillus thuringiensis in mice inoculated intraperitoneally]. | 1975 | 808356 | |
[elimination of the thermostable exotoxin from b. thuringiensis after ingestion in the mouse]. | when ingested by mice, the thermostable exotoxin is quickly eliminated in the "faeces", without fixation on the liver, the heart, the spleen or the kidneys. by quantitative study with a radio-active tritium-labelled exotoxin, this elimination is shown to be almost complete in 24 hours. | 1975 | 809165 |
[the mycotoxin sensitivity of several bacillus thuringiensis (berliner) strains sensitive and resistant to aflatoxin b1]. | antimicrobial activity of pure preparations of seven mycotoxins, coumarin and dicoumarin, was studied against various strains of bacillus thuringiensis (berliner). the acquisition of resistance to aflatoxin b1, by a new strain designated "stable variant", obtained in the presence of mycotoxin lethal dose, is very specific. however, a relation seems to exist between aflatoxin b1 susceptibility and sensitivity to compounds which possess a double furan ring. in vitro, coumarin exhibits an inhibitor ... | 1975 | 809186 |
[inhibition of cytotoxic effects of b1 aflatoxin towards the bacterial cell by coumarin. role of other interfering factors]. | the coumarin has with some limits a repressive effect on the antibacterial power of aflatowin b1. it behaves towards cells of bacillus thuringiensis (berliner) treated by the myocotoxic, like a curative agent. therefore, the reaction of microbial cells on the cytotoxic effect of aflatoxin b1 depends on the aeration of the culture and chiefly on the "inoculum effect". | 1975 | 811410 |
[a bacillus thuringiensis berliner mutant resistant to oxytetracycline, with temperature-sensitive sporulation]. | sporulation of an oxytetracycline-resistant mutant is blocked at 37 degrees c and delayed at 30 degrees c; shift up and shift down (30-37 degrees c) and assays of some extra- and intracellular enzymes show that an early event (stage ii) is concerned. | 1975 | 813848 |
inhibition of adenyl cyclase by an exotoxin of bacillus thuringiensis. | 1975 | 163006 | |
metalloprotease from bacillus thuringiensis. | bacillus thuringiensis var. kurstaki was shown to produce an extracellular, metal chelator-sensitive protease during the early stages of sporulation. protease production in nutrient broth was dependent upon supplementation with mn2+ or ca2. the addition of ca24 was required for enzyme stabilization... | 1975 | 241290 |
determination of exotoxin in bacillus thuringiensis cells. | the presence of exotoxin in bacillus thuringiensis was demonstrated and its quantity in the cells determined. the concentration of exotoxin in the producing microorganism is approximately half the concentration of atp. exotoxin is produced at such a rate that the cell excretes 1/5 to 1/4 of its exotoxin content into the medium per minute. | 1975 | 123390 |
interaction of bacillus thuringiensis and nuclear polyhedrosis virus in spodoptera exigua. | 1975 | 773648 | |
coding capacity of the transcript products synthesized in vitro by the rna polymerases from bacillus thuringiensis. | 1976 | 779781 | |
translation of a stable mrna fraction from sporulating cells of bacillus thuringiensis in a cell-free system from escherichia coli. structural homologies between the native crystal protein and the products synthesized in vitro. | stable messenger rnas from sporulating cells of bacillus thuringiensis were translated in vitro in cell free system from e. coli. the mrna fraction used was extracted from cells treated at t4 of sporulation time with rifampicin for 10 minutes at 30degreesc. this stable-mrna enriched fraction directed the synthesis of polypeptides showing a size distribution up to 40,000 daltons. structural homologies between the in vitro products labeled with [3h]-valine and the native crystal protein labeled wi ... | 1976 | 782551 |
does cyclic amp have a role in the pathogenesis of fever in the rabbit? | 1976 | 180436 | |
electron microscope study of sporulation and parasporal crystal formation in bacillus thuringiensis. | a comprehensive ultrastructural analysis of sporulation and parasporal crystal development is described for bacillus thuringiensis. the insecticidal crystal of b. thuringiensis is initiated at the start of engulfment and is nearly complete by the time the exosporium forms. the crystal and a heretofore unobserved ovoid inclusion develop without any clear association with the forespore septum, exosporium, or mesosomes. these observations contradict previous hypotheses that the crystal is synthesiz ... | 1976 | 182671 |
fine structure of the baccilus thuringiensis spore. | the thin-sectioned spore of bacillus thuringiensis resembles that of bacillus cereus in fine structure. planar inclusions occur between the exosporium and spore coat and are structured differently from the parasporal crystal outside the exosporium. | 1976 | 185959 |
the effect of an inhibitor of adenylate cyclase on the development of pyrogen, prostaglandin and cyclic amp fevers in the rabbit. | an exotoxin of bacillus thuringiensis known to inhibit adenylate cyclase in vitro has been used to investigate the role of cyclic amp in the pathogenesis of fever in the rabbit. intra-hypothalamic microinjections of the exotoxin are non-pyrogenic and significantly attenuate the hyperthermia caused by intrahypothalamic microinjections of both bacterial pyrogen (endotoxin) and prostaglandin e1. the hyperthermia produced by dibutyrl cyclic amp is not affected by the exotoxin. these results support ... | 1976 | 189286 |
[ultrastructure of bacteriophages specific for bacillus thuringiensis var. galleriae]. | fine structure of the phage specific for bacillus thuringiensis var. galleriae was studied. the head of the phage is of the elongated hexagonal shape, 600 x 360 a. the tail is 400 x 40 a large and has a thickening in the basal part (70 x 120 a). a so-called "collar" structure is found at the site where the head joins the tail. this a disc-shaped plate with a diameter of 120 a and a hole in the centre; 12 fillaments are attached to it radially and terminated with tines. it is possible that the st ... | 1976 | 979688 |
bacillus thuringiensis enzyme-digested delta endotoxin: effect on cultured insect cells. | cells from three insect cell lines responded to the enzyme-digested delta endotoxin of bacillus thuringiensis with swelling, lysis, and vesicle formation. sufficient toxin was taken up in 1 minute to cause half-maximal cell damage. cytoxic activity was neutralized by specific antiserum to the endotoxin. | 1976 | 982053 |
mode of action of a purified antitumor protein from the proteinaceous crystal of baccillus thuringiensis subsp. thuringiensis on yoshida ascites sarcoma cells. | a purified antitumor protein from the proteinaceous crystal of bacillus thuringiensis subsp. thuringiensis inhibits the growth of yoshida ascites sarcoma both in vivo and in vitro. exogenous respiration of the tumor cells was unaffected by the protein at a concentration as high as 500 mug/ml. the antitumor protein inhibits the uptake and incorporation of labeled precursors into macromolecules. however, the ratio of incorporation over uptake is not affected by the protein. further, the protein br ... | 1976 | 984772 |
commercial formulations of bacillus thuringiensis for control of indian meal moth. | doses of four commercial formulations and one experimental formulation of bacillus thuringiensis berliner were mixed with the diet used to rear colonies of the indian meal moth plodia interpunctella (hübner). indian meal moth eggs were introduced to the treated diet, and the resultant adult emergence was tabulated. the experimental formulations ranked as follows in efficacy in controlling the indian meal moth: dipel (50% lethal concentration [lc50], 25 mg/kg) greater than bactospeine wp (lc50, 1 ... | 1976 | 984828 |
[pathological changes in fleas caused by the action of bacillus thuringiensis exotoxin]. | pathogenic effect of the crystalline exotoxine of bacillus thuringiensis on the larvae of fleas was investigated. deformities and changes in the functions of organs and tissues caused by the exotoxin in larvae and imago developed from the latter result in the premature death of the individuals (to 100%) or in the sterility of the remaining fleas. | 1976 | 986601 |
[analytical investigations of the delta-endotoxin of bacillus thuriginiensis (author's transl)]. | biochemical properties of the crystalline delta-endotoxin of bacillus thuringiensis, such as stability and solubility in different solvents, were investigated. the dissolved compounds were characterized by gel-electrophoresis and gel-filtration. covalent and non-covalent bonds are responsible for the crystallisation of the protein molecules. the solubilisation of crystals with non-enzymatic solvents led to high molecular weight products (mw greater than or equal to 800,000) and to components wi ... | 1976 | 989654 |
the exotoxin of bacillus thuringiensis: a new c-mitotic agent. | thuringiensin a, an exotoxin from bacillus thuringiensis, a constituent of the microbial insectide thuricide has been found to inhibit mitotic spindle, condense and scatter chromosomes. it may therefore be a promizing tool in future cell biological studies. | 1976 | 991999 |
antitumor immunity against yoshida ascites sarcoma after treatment with the proteinaceous crystal of bacillus thuringiensis var. thuringiensis. | 1976 | 992771 | |
evidence for two immune inhibitors from bacillus thuringiensis interfering with the humoral defense system of saturniid pupae. | mutants of bacillus thuringiensis lacking either beta-exotoxin or gamma-endotoxin were compared for their virulence using pupae of a giant silk moth. known doses of viable log-phase bacteria were injected, and the response was followed as the number of viable bacteria in the hemolymph. the results obtained imply that, in the system used, neither the beta-exotoxin nor the gamma-endotoxin and the sporeforming ability are of importance for virulence. results with sterile culture filtrate from b. th ... | 1976 | 992874 |
[electron microscopic study of intracellular growth of phage specific for bacillus thuringiensis var. galleriae]. | ultrathin sections of bacillus thuringiensis var. galleriae infected by a specific phage were studied by electron microscopy. stages of the phage reproduction within the cell, from adsorption of phage particles to liberation of the phage from the cell, have been revealed. growth of the phage follows that of dna-containing phages. the yield of the phage is very high. | 1976 | 1004276 |
[relationship between exoprotease activity, spore and crystal formation and virulence of bacteria of the group bacillus thuringiensis]. | experiments were carried out to investigate the activity, spore and crystal formation and virulence of bacillus thuringiensis. bacteria of this group showed different exoprotease activity. strains of one serotype differed in the level and time of occurrence of peak enzyme activity. exoprotease activity of the bacteria cannot be indicative of their toxicity because it did not correlate with their virulence. exoprotease activity can be used to characterize bacteria during their classification and ... | 1976 | 1026937 |
bacterial membrane transport of beta-exotoxin, an anti-metabolite of rna synthesis. | 1976 | 815825 | |
response of bacillus thuringiensis to bacteriophage cp-51. | bacteriophage cp-51, a transducing phage of bacillus cereus was able to replicate on all eight varieties of bacillus thuringiensis tested. three general plaque types were observed on each strain although one type predominated on each strain. the plaque size was uniform for each strain regardless of plaque type. the bacterial strain used as source of the phage had no effect on plaque type or size found on any host strain. cp-51 was stable in infected spores of b. thuringiensis var. kurstaki for a ... | 1976 | 816441 |
[role of phagocytosis and soluble antibacterial factors in experimental immunization of locusta migratoria]. | last instar larvae of locusta migratoria can be protected ("vaccinated") against lethal doses of bacillus thuringiensis by previous injections of low doses of this pathogen. if iron saccharate is injected in to larvae prior to the administration of the "vaccinating" dose of bacillus thuringiensis, no antibacterial protection can be induced. injection of iron saccharate in to "vaccinated" larvae does not interfere with the induced protection; such larvae resist lethal doses. | 1976 | 821627 |
[characteristics of bacillus thuringiensis berliner mutants, resistant to tetracycline, having retained or lost the ability to sporulate]. | quantitative analysis of extracellular protease, nadh-oxydase and glucose-deshydrogenase show that, from two mutants selected by their oxytetracycline resistance, one sporulates better than the parent strain, the other, which sporulates at 10(-5), has a stable extracellular protease activity and appears to be blocked at stage o-i. | 1976 | 821636 |
[biochemical determination of b. thuringiensis thermostabile, exotoxin, using the inhibition of bacterial rna polymerases]. | this method can determine amounts of exotoxin near 1 mug. used on the autoclaved filtrates of cultures of b. thuringiensis var. thuringiensis (serotype 1 type-strain), it shows an excretion rate of 250 to 300 mug per millilitre. the results on different exotoxin-producer strains give a perfect parallelism with the results obtained by bio-assays on insects, but the biochemical method is more accurate and reproducible. this biochemical technique allows us, for the first time, to place the exotoxin ... | 1976 | 822953 |
[the effect of repeated amputations on planarian regeneration in the presence of the heat-stable toxin from bacillus thuringiensis]. | the exotoxin of bacillus thuringiensis, which is an inhibitor of rna synthesis, inhibits planarian regeneration. planarians which have been cut twice at the same level are able to regenerate after the second section in the presence of the toxin. this indicates that the first amputation stimulates a synthesis of stable rnas which thus are available for regeneration at the moment of the second section. | 1976 | 825282 |
[physicochemical characteristics of dna and the amino acid composition of bacillus phages]. | from five bacillus phages nucleic acids were isolated and identified as double-stranded dna of the at-type. their melting point was 85-86.5 degrees c, molecular weight 23.4-43.7x10(6) daltons. the content of dna in phage particles was 39.9-44.0%, the content of protein 55.7-56.1%. the examination of the amino acid composition in phages 125/50 and 617 revealed a low level of serine and the lack of methionine, while the remaining amino acid composition of bacillus phages was close to that of e. co ... | 1976 | 827122 |
effects of sesquiterpene lactones on the growth of bacillus thuringiensis. | two sesquiterpene lactones, hymenovin and tenulin, were tested for their effect on growth of two strains of bacillus thuringiensis. growth of both strains was 98% inhibited by 0.6 mg of tenulin per ml, but only 15 to 20% was inhibited by the same level of hymenovin. hymenovin appeared to have a mutagenic effect on b. thuringiensis cultures resulting in production of several variant strains. some of the variant strains had lost their ability to form spores and crystals. hymenovin also induced b. ... | 1976 | 1259409 |
dormant spores of bacillus thuringiensis contain an inhibition of rna polymerase. | 1976 | 1259762 | |
[antibacterial effect of various mycotoxins and fungal metabolites against bacillus thuringiensis (berliner) strains sensitive or resistant to aflatoxin b1]. | antimicrobial activity of pure preparations of mycotoxins and fungal metabolites was studied against strains of bacillus thuringiensis (berliner). two resistant strains, called stable-variant, were isolated after treatment with high concentrations of aflatoxin b1. these strains were then resistant also towards compounds with a double furan system (aflatoxins b1, b2, g1, g2, and sterigmatocystin). | 1976 | 1277008 |
chitinase of bacillus thuringiensis. | strains of bacillus thuringiensis were shown to hydrolyse various forms of chitin around growing colonies on a solid medium. in conditions of submerged cultivation on a medium containing demineralized crab shells, bac. thuringiensis var. caucasicus inmi arm. 837 manifests the chitinolytic activity at the beginning of the stationary growth phase. the activity of chitinase which is of a constitutive nature increases when the bacterium is cultivated at ph 7.2. the maximum rate of hydrolysis of coll ... | 1976 | 13274 |
application of bathurin and borax in the biological control of monomorium pharaonis in housing estates. | of two apartment houses infested with ants of the species monomorium pharaonis, one was treated with a biological, the other with a chemical substance. in the first case we used bathurin in combination with borax at a concentration of 1.3% for both substances. in the first week, borax was added to the bait (minced beef), within the next three weeks, the bait was mixed with bathurin. the procedure was repeated 5 times. after this period, we achieved complete control of the ants on the infested pr ... | 1976 | 62524 |
proteins of bacillus thuringiensis delta-endotoxin crystals. | pure crystals (at least 99% purification) of sigma-endotoxin were isolated from bac. thuringiensis var. galleriae. the complete dissolution of crystals might be achieved by the increase of ph up to 12 and higher or by a combined action of s = s-reducing and denaturing agents. electrophoresis of the solubilized crystal proteins in 5% polyacrylamide gels containing 0,1% sodium dodecyl sulfate and 8 m urea reveals two major bands corresponding to molecular weights of 120000--140000 (65%) and 65000 ... | 1977 | 21000 |
[construction of partial denaturation charts for dna with random base distribution]. | by measuring dna temperature transition profiles the evidence was obtained that dna of phages tg9 and ob infecting bacillus thuringiensis and brevibacterium flavum respectively have a random base distribution. after alkaline treatment in the presence of 10 per cent formaldehyde at room temperature partially denatured molecules of phage tg9 and ob dna's with different degrees of denaturation were obtained. using electron microscopy the maps of distribution of melted regions along these dna's were ... | 1977 | 36554 |
[electron microscopic study of the cells of variants of bacillus thuringiensis var. galleriae forming colonies with altered morphology]. | colonial-morphological variants of bacillus thuringiensis var. galleriae were studied by electron microscopy. the s variant was found to be asporogenous, whereas the r variant produced mature spores. morphological modifications in the cell were followed in the course of sporulation. spores were shown to have thin filamentous and tubular protrusions which had not been so far described in crystalliferous bacteria. preparations from colonies of the r variant contained peculiar structures which rese ... | 1977 | 600105 |
[antibacterial properties of aflatoxin b1: cytotoxic effects on bacilus thuringiensis (berliner)]. | in a sensitive strain of bacillus thuringiensis (berliner), aflatoxin b1 inhibited growth with a dose above 5.0 microgram/ml. with subinhibitory levels (0.5 - 5.0 microgram/ml) physiological damage (decreased growth rate) and cellular alteration (filamentous cells) were noted. with these doses the mycotoxin disturbs various metabolisms : dna synthesis (gradual blockage of the specific bacteriophage multiplication and dispersion of nuclear apparatus in giant cells); protein synthesis (decrease of ... | 1977 | 613933 |
[physico-chemical properties of several phages of bacillus thuringiensis]. | a study was made of biological and physico-chemical properties of phages of bac. thuringiensis as well as of a number of parameters of nucleic acids isolated from these phages. the phages contain double-stranded dna. molecular weights of dna from three phages--tg9, tg10 and tg13 have been determined by two independent methods: by measuring the contour length of dna, from the sedimentation constant and for dna of phage tg10 also by endonuclease ecri hydrolysis. these methods gave similar results. ... | 1977 | 618332 |
membrane fractions from the outer layers of spores of bacillus thuringiensis with toxicity to lepidopterous larvae. | two membrane fractions, f1 and f2, have been purified from the outer layers of spores of bacillus thuringiensis. both fractions contain 6-7% cysteine and appear to be similar in composition. amino acids account for about 75% of the dry weight, carbohydrate for about 2% and lipids for about 25%. the fractions are both toxic to pieris brassicae and the toxicity is inactivated by antiserum to the toxic crystal of bacillus thuringiensis. the fractions can be distinguished by examination under the el ... | 1977 | 402265 |
[characteristics of phages of spore-forming bacteria isolated from the soil]. | phages lyzing spore forming bacteria were isolated from soil, and their biological properties and fine structure were studied. the spectrum of lytic activity was determined as well as parameters of the intracellular phage growth. the burst size of the phage varies from 8 to 725 particles per infected cell, the latent period lasts 25-100 min for various phages. according to the data of electron microscopy, the phages are divided into three morphological groups. the phages tg7, ar13 and bpp10 have ... | 1977 | 408587 |
[morphologic alterations induced by aflatoxin b1 in bacillus thuringiensis (berliner)]. | significative ultrastructural alterations shown in aflatoxin b1, treated cells of bacillus thuringiensis are: increase of number of mesosomes which are hypertrophied, decrease of ribosomes and formation of imperfect cell septa. | 1977 | 409554 |
[mitogenic action of the thermostable exotoxin of bacillus thuringiensis]. | 1977 | 410525 | |
[mutants of bacillus thuringiensis with altered sporulation: morphologic responses to several treatments applied in the exponential phase]. | during log-phase of growth, wild strain and and oxytetracycline-resistant mutants with altered sporulation exhibit distinct responses to some treatments, autolysis, osmotic pressure, ultraviolet irradiation and bacteriophage infection. | 1977 | 411590 |
cytogenetic hazards from agricultural chemicals. i. a preliminary study on the responses of root meristems to exotoxin from bacillus thuringiensis a constituent of a microbial insecticide, thuricide. | it reported for the first time that the exotoxin, thuringiensin a, from bacillus thuringiensis, a component of the insecticide thuricide, inhibits spindle and cytokinesis and induces micronuclei, chromocentric nuclei and minor deviations in spindle activity. the binucleate cells also undergo mitosis yielding biprophases and bimetaphases. spindle seems to have been inhibited even in bimetaphase. microtubular systems and chromosomes are implicated as the primary targets. most effects resemble thos ... | 1977 | 840238 |
characterization of the entomocidal parasporal crystal of bacillus thuringiensis. | the parasporal crystalline protoxin of bacillus thuringiensis contains a single glycoprotein subunit that has a molecular weight of approximately 1.2 x 10(5). the carbohydrate consists of glucose (3.8%) and mannose (1.8%). at alkaline ph, the proendotoxin is apparently solubilized and activated by an autolytic mechanism involving an inherent sulfhydryl protease that renders the protoxin insecticidal. activation generates protons, degraded polypeptides, sulfhydryl group reactivity, proteolytic ac ... | 1977 | 853031 |
bioassay for homogeneous parasporal crystal of bacillus thuringiensis using the tobacco hornworm, manduca sexta. | a method for determining the toxicity of bacillus thuringiensis subsp. kurstaki parasporal crystal to the tabocco hornworm, manduca sexta, is described. the use of both mortality and weight loss data have provided a highly sensitive and reproducible bioassay that can be used to compare relative toxicities of crystals from other subspecies as well as toxic components contained therein. | 1977 | 869534 |
[effect of aflatoxin b1 on the enzymatic activities of bacillus thuringiensis (berliner)]. | at 20 degrees c, aflatoxin b1, at a sublethal dose, decreases the activity of alkaline phosphatase (ec 3.1.3.1), alpha-glucosidase (ec 3.2.1.20), esterase (ec 3.1.1.1), chymotrypsin (ec 3.4.21.1), leucine aminopeptidase (ec 3.4.11.1), and phosphoamidase (ec 3.9.1.1) biosynthesis in bacillus thuringiensis (berliner). in contrast, at 41 degrees c no significant decrease was observed. at this temperature, the mycotoxin is not destroyed or metabolized and bacterial cells are resistant to the toxin. | 1977 | 884628 |
[isolation and preliminary characteristics of the extrachromosomal elements of bacillus thuringiensis dna]. | centrifugation of bacillus thuringiensis 351 dna in cscl-ethidium bromide density gradient has revealed, besides the main band of chromosomal dna (p = 1.56 g/cm2), some additional bands with higher density, which usually correspond cicle superhelix dnas, are revealed. 6 discrete bands are observed under electrophoresis of total fraction of minor dna bands, which suggests, that the preparation contains cicle dnas of different size. the treatment with dnase results in the appearance of 3 bands cor ... | 1977 | 892434 |
[first results of the antibacterial defense reactions of locusta migratoria larva and imago]. | 1977 | 900783 | |
[polylysogeny in bacillus thuringiensis var. galleriae cultures]. | the culture of bacillus thuringiensis var. galleriae was shown to be polylysogenic. as has been found earlier, the culture has a phage with the original structure of particles whose tail possesses a special substructure called a "collar". the phage was described in detail as a phage of bac. thuringiensis 1-97 with a "collar". further studies of the culture of bac. thuringiensis var. galleriae 1-97 has shown that it also contains another phage which differs sharply from the first one in the morph ... | 1977 | 909472 |
characterization of an intracellular protease isolated from bacillus thuringiensis sporulating cells and able to modify homologous rna polymerase. | 1977 | 923560 | |
absence of beta-exotoxin in thuricide preparations. | the biological insecticide thuricide is produced from b. thuringiensis, berliner, var. kurstaki (serotype 3a, 3b), a bacterial strain which does not synthesize exotoxin. thus, our product is devoid of any c-mitotic or mutagenic potentiality such as is to be found in exotoxin. | 1977 | 923744 |
evaluation of bacillus thuringiensis as a biocide of blackfly larvae (diptera: simuliidae). | 1977 | 925365 |