Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| isolation and characterization of 5s rna-protein complexes from bacillus stearothermophilus and escherichia coli ribosomes. | 1972 | 4567807 | |
| specific recognition of non-initiator regions in rna bacteriophage messengers by ribosomes of bacillus stearothermophilus. | 1973 | 4570382 | |
| mode of action of n-tosyl-l-phenylalanylchloromethane on the elongation protein-synthesizing s 3 factor from bacillus stearothermophilus. | 1973 | 4570973 | |
| survival of microorganisms in laundered polyester-cotton sheeting. | the effects of wash-water temperature, cold-water or regular detergent, wash-cycle design, drying, and drying temperature on survival of four microorganisms on polyester-cotton sheeting were examined. escherichia coli t3 bacteriophage survived washing at 24, 35, 46, and 57 c, but not at 68 c. serratia marcescens survived only the lowest three wash temperatures. levels of residual staphylococcus aureus were diminished at the highest two wash temperatures, but survival was substantial even at 68 c ... | 1973 | 4572894 |
| inhibition of spore outgrowth and vegetative growth of bacillus stearothermophilus by dipicolinate. | spores of bacillus stearothermophilus germinated in the presence of sodium dipicolinate (dpa) but did not elongate after the emergence stage of outgrowth. vegetative growth also was inhibited by na(2)dpa. | 1973 | 4574703 |
| [demonstration of "free spores" of bacillus stearothermophilus through synchronous sporulation with subsequent endotrophic lysis of sporangia]. | 1973 | 4581213 | |
| discriminatory ribosome rebinding of isolated regions of protein synthesis initiation from the ribonucleic acid of bacteriophage r17. | to determine whether bacterial ribosomes recognize a distinguishing feature in the immediate vicinity of actual initiator codons or are directed to these sites through involvement of other portion(s) of the mrna molecule, the interaction between ribosomes and defined (32)p-labeled initiator fragments from r17 rna was studied. when incubated with mixtures of the three sites, ribosomes from bacillus stearothermophilus (which initiate only the a protein on intact phage rna) are able to select out t ... | 1973 | 4582190 |
| the amino acid sequence of the n-terminal region of some 30s ribosomal proteins from escherichia coli and bacillus stearothermophilus: homologies in ribosomal proteins. | 1973 | 4583836 | |
| a study of the thermal stability of ribosomes and biologically active subribosomal particles. | 1. the ability of escherichia coli ribosomes to function in poly(u)-directed protein synthesis was measured at elevated temperatures by using thermostable supernatant factors from bacillus stearothermophilus. the amount of polyphenylalanine synthesized at 55 degrees c was about the same as at 37 degrees c, but the rate of synthesis was increased approximately fivefold. at 60 degrees c the activity of the ribosomes was halved. 2. e. coli ribosomes can sustain the loss of approx. 10% of the double ... | 1973 | 4584137 |
| study of conditions for the inhibitory effect of n-tosyl-l-phenylalanylchloromethane on protein synthesis and the possibility of existence of different forms of the elongation factor s3 in bacillus stearothermophilus. | 1973 | 4587077 | |
| correlation between 30s ribosomal proteins of bacillus stearothermophilus and escherichia coli. | 1973 | 4589348 | |
| specific modification of arginyl-transfer ribonucleic-acid synthetase from bacillus stearothermophilus as a result of substrate binding. | 1973 | 4589541 | |
| proceedings: regulation of gluconeogenesis in bacillus stearothermophilus. | 1973 | 4604960 | |
| identification of chloramphenicol-binding protein in escherichia coli ribosomes by affinity labeling. | monoiodoamphenicol, a synthetic analogue of chloramphenicol, has been shown by competition experiments with chloramphenicol and lincomycin to bind at the same site of 70s ribosomes as chloramphenicol. at - 2 degrees it forms a 1:1 complex with 70s ribosomes having a value of k (7.5 x 10(4) m(-1)) that is one order of magnitude lower than that of chloramphenicol. at 37 degrees , monoiodoamphenicol irreversibly inhibits the protein-synthesizing activity of e. coli ribosomes. it is shown that the a ... | 1973 | 4599619 |
| use of bacillus stearothermophilus as a biological indicator. | 1973 | 4686286 | |
| differential heat stabilities of bacillus amylase. | seventeen cultures representing eight strains of bacillus coagulans and twelve cultures representing eight strains of bacillus stearothermophilus did not yield a culture that would produce amylase at both 37 and 55 c. | 1973 | 4688668 |
| the effect of growth temperatures on the in vivo ribose methylation of bacillus stearothermophilus transfer rna. | 1973 | 4689778 | |
| restriction and modification of bacteriophage in bacillus stearothermophilus. | host-controlled restriction and modification of tp-1c phage and infectious phage dna occurs in bacillus stearothermophilus and is subject to control by tp-8 or tp-12 prophage. | 1973 | 4700514 |
| test objects containing bacillus stearothermophilus spores for the monitoring of antimicrobial treatment in steam autoclaves. results obtained with commercial preparations and with the authors' own test objects. | 1973 | 4702494 | |
| d-alanine carboxypeptidases of bacillus stearothermophilus: solubilisation of particulate enzymes and mechanism of action of penicillin. | 1973 | 4710761 | |
| mode of action of n-tosyl-l-phenylalanylchloromethane on the elongation protein-synthesizing s 3 factor from bacillus stearothermophilus. | 1973 | 4711185 | |
| metabolism of phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin of bacillus stearothermophilus. | the total phospholipid content of bacillus stearothermophilus was constant during exponential growth, increased during the transition from the exponential to stationary phase of growth, and then slowly increased during the stationary phase. the first increase was a result of an increase in phosphatidylethanolamine; the second was a result of an increase in cardiolipin. cessation of aeration of an exponentially growing culture or suspension in a nongrowth medium resulted in an immediate reduction ... | 1973 | 4712568 |
| preferred sites of digestion of a ribonuclease from enterobacter sp. in the sequence analysis of bacillus stearothermophilus 5s ribonucleic acid. | 1973 | 4719125 | |
| metabolism of phospholipids in bacillus stearothermophilus. | 1973 | 4756899 | |
| isolation and characterization of a thermophilic glucokinase from bacillus stearothermophilus. | 1973 | 4763322 | |
| 6-phosphogluconate dehydrogenase from bacillus stearothermophilus. | 1973 | 4772689 | |
| [determination of reaction kinetic data on the vapor inactivation of bacillus stearothermophilus spores, endotrophically influenced by bivalent cations]. | 1973 | 4774658 | |
| mode of action of the macrolide-type antibiotic, chlorothricin. kinetic study of the inhibition of pyruvate carboxylase from bacillus stearothermophilus. | 1973 | 4775066 | |
| letter: crystallization and preliminary x-ray diffraction studies on tyrosyl-transfer rna synthetase from bacillus stearothermophilus. | 1973 | 4784896 | |
| effect of copper on the growth of bacillus stearothermophilus. | 1973 | 4801279 | |
| association of ribosomal subunits. 3. properties of the 30 s-50 s couples produced in vitro by the association factor from bacillus stearothermophilus. | 1973 | 4633533 | |
| the function of the two subunits of thermophilic aminopeptidase i. | the thermophilic high-molecular-weight aminopeptidase i (ec 3.4.11.1) from bacillus stearothermophilus is composed of 12 subunits of two different types (alpha,beta) which can combine in various ratios. only one type of subunit (alpha) is needed for the degradation of neutral peptides, but dipeptides having amino-terminal aspartic or gultamic acid are substantially hydrolyzed only by enzyme containing the other subunit (beta) as well. asp-gly inhibits the enzymatic hydrolysis of glutamic acid 1- ... | 1973 | 4521203 |
| isolation of different thermophilic enzymes from bacillus stearothermophilus. | 1973 | 4354752 | |
| atpase and gtpase activities associated with a specific 5s rna-protein complex. | an rna-protein complex consisting of 5s rna and two ribosomal proteins, b-l5 and b-l22, was isolated from bacillus stearothermophilus ribosomes and found to be active in gtp hydrolysis. this activity was not influenced by elongation factor g. further analysis of this complex showed that it was also able to hydrolyze atp. inhibition studies revealed that atp was a noncompetitive inhibitor of gtp and that gtp was also a noncompetitive inhibitor of atp, indicating that two different enzymatic sites ... | 1973 | 4355369 |
| glyceraldehyde 3-phosphate dehydrogenase of bacillus stearothermophilus. kinetics and physicochemical studies. | 1973 | 4358785 | |
| functional correspondence between 30s ribosomal proteins of escherichia coli and bacillus stearothermophilus. | 30s ribosomal proteins from bacillus stearothermophilus (b. proteins) have been fractionated and characterized with respect to their ability to replace various e. coli 30s proteins (e. proteins) in the e. coli 30s ribosome reconstitution system. the functional counterparts of all the e. proteins, except s1, s6, s9, and s13, have been tested. in all cases, b. proteins can substitute for e. proteins. several purified b. proteins are chemically different from their functionally homologous e. protei ... | 1973 | 4197630 |
| additional antibiotic inhibitors of peptidoglycan synthesis. | diumycin, janiemycin, nisin, and subtilin inhibited peptidoglycan synthesis catalyzed by particulate enzyme systems from bacillus stearothermophilus and escherichia coli. all of these, except for nisin, also induced accumulation of the lipid intermediate in peptidoglycan synthesis. concentrations required for 50% inhibition of peptidoglycan synthesis were less than 0.1 mug/ml for diumycin and in the range of 10 to 100 mug/ml for janiemycin, nisin, and subtilin in both organisms. the discrepancy ... | 1973 | 4202341 |
| interactions of arginyl- and isoleucyl-trna ligases from bacillus stearothermophilus with their substrates. | 1973 | 4128302 | |
| membranes of bacillus stearothermophilus: factors affecting protoplast stability and thermostability of alkaline phosphatase and reduced nicotinamide adenine dinucleotide oxidase. | protoplasts of bacillus stearothermophilus nca 1503-4r are resistant to osmotic rupture and are not sensitive to mechanical manipulation. protoplast stability is maintained by divalent cations. the thermostability of protoplasts is enhanced when the cells are grown at elevated temperatures. the membrane content of the cell and the protein-to-lipid ratio of the membrane increases as the growth temperature is increased. the membrane-bound nicotinamide adenine dinucleotide (reduced form) oxidase sy ... | 1973 | 4145864 |
| release of dipicolinic acid and calcium and activation of bacillus stearothermophilus spores as a function of time, temperature and ph. | 1973 | 4146587 | |
| isolation of mutants of bacillus stearothermophilus blocked in catabolic function. | 1973 | 4149949 | |
| arginyl-trna synthetase from bacillus stearothermophilus: heat inactivation and substrate induced protection. | 1973 | 11946943 | |
| release of the dipicolinic acid (dpa) from spores of bacillus stearothermophilus, b. megaterium and b. anthracis in presence of dyes. | 1974 | 4151214 | |
| on the conformation of nadp-dependent isocitrate dehydrogenase from bacillus stearothermophilus. | 1974 | 4151887 | |
| glutamine synthetase of bacillus stearothermophilus. i. purification and basic properties. | 1974 | 4152179 | |
| glutamine synthetase of bacillus stearothermophilus. ii. regulation and thermostability. | 1974 | 4152245 | |
| purification and properties of glutamine synthetase from bacillus stearothermophilus. | 1974 | 4152611 | |
| conformational change with temperature and thermostability of glutamine synthetase from bacillus stearothermophilus. | 1974 | 4153301 | |
| separation of transfer rna's from bacillus subtilis, bacillus stearothermophilus and chicken liver by polyacrylamide gel electrophoresis. | 1974 | 4209781 | |
| purification and characterization of the thermophilic d-alanine carboxypeptidase from membranes of bacillus stearothermophilus. | 1974 | 4211099 | |
| binding of (14c)penicillin g to the membrane-bound and the purified d-alanine carboxypeptidases from bacillus stearothermophilus and bacillus subtilis and its release. | 1974 | 4214007 | |
| characterization of a bacteriophage-induced, host-specific lytic enzyme from lysates of bacillus stearothermophilus infected with bacteriophage tp-8. | phage tp-8 lysates of bacillus stearothermophilus 4s or 4s(8) contain lytic activity exhibiting two ph optima, one at ph 6.5 and the other at ph 7.5. using a variety of fractionation procedures, the two lytic activities could not be separated. at ph 7.5 the lytic enzyme is an endopeptidase which hydrolyzes the l-alanyl-d-glutamyl linkage in the peptide subunits of the cell wall peptidoglycan and at ph 6.5 it exhibits n-acetylmuramidase activity. endopeptidase activity is inhibited by nacl and ne ... | 1974 | 4218232 |
| a homogeneous, thermostable deoxythymidine kinase from bacillus stearothermophilus. | 1974 | 4371810 | |
| correlation between thermal death and membrane fluidity in bacillus stearothermophilus. | paramagnetic resonance spectra of spin labels partitioned into spheroplast membranes of bacillus stearothermophilus indicate lateral lipid phase separations. cells adjust their lipid composition in response to temperature changes so that the same change of state in membrane phospholipids is achieved at the respective growth temperature. a temperature-sensitive mutant that fails to change its lipid composition above a certain temperature can survive only up to the higher temperature boundary for ... | 1974 | 4372606 |
| thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus: ii. kinetic and immunokinetic analysis. | 1974 | 4376605 | |
| purification and metal ion activation of an aminopeptidase (aminopeptidase ii) from bacillus stearothermophilus. | 1974 | 4407342 | |
| bacillus stearothermophilus in soils of iceland. | bacillus stearothermophilus was found in icelandic soils. of the 33 strains studied, only four grew at 25 c. temperatures as high as 27 c in bare soil at a depth of 1 to 1.5 cm were measured at the collection site. however, to demonstrate growth the cultures had to be incubated for 3 weeks, and soil temperatures remained at 27 c only for shorter periods of time. | 1974 | 4421871 |
| altered 30s ribosomal proteins in streptomycin resistant, dependent and independent mutants of bacillus stearothermophilus. | 1974 | 4427590 | |
| teichoic acid synthesis in bacillus stearothermophilus. | 1. particulate enzyme preparations obtained from bacillus stearothermophilus b65 by digestion with lysozyme were shown to catalyse teichoic acid synthesis. with cdp-glycerol as sole substrate the preparations synthesized 1,3-poly(glycerol phosphate). it was characterized by alkaline hydrolysis, by glucosylation to the alkali-stable 2-glucosyl-1,3-poly(glycerol phosphate) with excess of udp-glucose and a bacillus subtilis marburg enzyme system, by degradation of this latter product with 60%hf and ... | 1974 | 4429546 |
| production of a new thermostable neutral alpha-galactosidase from a strain of bacillus stearothermophilus. | 1974 | 4429799 | |
| phosphoglycerate kinase of bacillus stearothermophilus. | 1974 | 4436288 | |
| relationship of soil constituents to spore counts and heat resistance of bacillus stearothermophilus. | 1974 | 4441977 | |
| thermophilic aminopeptidase from bacillus stearothermophilus. iv. | 1974 | 4455642 | |
| [heat resistance of bacillus stearothermophilus spores depending on their dipicolinic acid and calcium concentration]. | 1974 | 4458258 | |
| the thermal resistance of bacillus stearothermophilus spores. the effects of temperature and ph of the heating medium. | 1974 | 4462138 | |
| [heat resistance of dryed bacillus stearothermophilus spores during storage at different temperatures and different relative humidities (author's transl)]. | 1974 | 4479585 | |
| metabolic differences in bacillus stearothermophilus grown at 55 degrees c and 37 degrees c. | 1974 | 4815911 | |
| amidation and cross-linking of the enzymatically synthesized peptidoglycan of bacillus stearothermophilus. | 1974 | 4822500 | |
| purification and properties of esterase from bacillus stearothermophilus. | 1974 | 4831626 | |
| tyrosyl transfer ribonucleic acid synthetase from bacillus stearothermophilus. preparation and properties of the crystallizable enzyme. | 1974 | 4831635 | |
| thermoadaptation of enzymes in thermophilic and mesophilic cultures of bacillus stearothermophilus and bacillus caldotenax. | 1974 | 4847049 | |
| reconstitution of 50 s ribosomal subunits from bacillus stearothermophilus. | 1974 | 4854050 | |
| [heat activation of bacillus stearothermophilus spores endotrophically treated with bivalent cations]. | 1974 | 4600359 | |
| incorrect aminoacylations involving trnas or valyl-trna synthetase from bacillus stearothermophilus. | 1974 | 4604598 | |
| a stringent factor activity associated with the 50 s ribosomal subunit of bacillus stearothermophilus. | 1974 | 4606153 | |
| procaryotic ribosomal proteins: n-terminal sequence homologies and structural correspondence of 30 s ribosomal proteins from escherichia coli and bacillus stearothermophilus. | 1974 | 4607606 | |
| a comparison of purified valyl-transfer ribonucleic acid synthetase from bacillus stearothermophilus and from escherichia coli. | the purification of valyl-trna synthetase from bacillus stearothermophilus is described. the protein was greater than 90% homogeneous on polyacrylamide-gel electrophoresis after more than 850-fold purification. it has a molecular weight of 110000, and no evidence was found for the presence of subunit structure. the properties of the purified enzyme were compared with those of purified valyl-trna synthetase from escherichia coli. the thermal stability, ph-stability and dependence of activity on t ... | 1974 | 4614793 |
| the fine-structural properties of the amino-terminal region of the 50s ribosomal "a" protein from bacillus stearothermophilus. | 1974 | 4619940 | |
| germination of spores of bacillus stearothermophilus induced by analogues of dipicolinate di-anion. | the structural specificity required for induction of germination of spores of bacillus stearothermophilus by analogues of dipicolinate in buffer at ph 5.5 is similar to that found previously with spores of bacillus megaterium and calcium chelate salts at ph 8. 4h-pyran-2,6-dicarboxylate, but no other analogue tested, is as effective as dipicolinate di-anion. | 1974 | 4591954 |
| amino-terminal sequences of some escherichia coli 30s ribosomal proteins and functionally corresponding bacillus stearothermophilus ribosomal proteins. | amino-terminal sequences of five purified escherichia coli 30s ribosomal proteins (s4, s9, s10, s16, and s20) were compared with those of their functionally corresponding bacillus stearothermophilus ribosomal proteins identified previously by the reconstitution technique. an automatic edman degradation method was used for sequence determinations. the sequence of the first 30 residues is presented, except that only the first 25 residues are shown for the s20 pair. substantial (40 to 70%) sequence ... | 1974 | 4595196 |
| cistron specificity of 30s ribosomes heterologously reconstituted with components from escherichia coli and bacillus stearothermophilus. | 1974 | 4597073 | |
| degradation of penicillin g to phenylacetylglycine by d-alanine carboxypeptidase from bacillus stearothermophilus. | d-alanine carboxypeptidase from bacillus stearothermophilus is a membrane-bound enzyme which is inhibited by covalent interaction with penicillin g. the penicilloyl enzyme spontaneously reactivates and simultaneously releases a penicillin g degradation product; 0.2 mumol of the latter was isolated after incubation of 4.2 mumol of [8-14c]penicillin g with 10 g of membrane protein. it was identified as phenylacetylglycine by chromatographic techniques, infrared spectroscopy, and mass spectrometry. ... | 1975 | 1059132 |
| purification and characterization of inorganic pyrophosphatase from bacillus stearothermophilus. | inorganic pyrophosphatase ec 3.6.1.1 was purified from bacillus stearothermophilus to a homogeneous state both ultracentrifugally and electrophoretically. ultracentrifugal analysis revealed that the molecular weight of the enzyme is 122,000 and the sedimentation coefficient (s0.34%/20, w) is 5.2s. the enzyme molecule in 0.1% sodium dodecylsulfate solution containing 1 mm 2-mercaptoethanol had an estimated molecular weight of 70,000 on the basis of sds-polyacrylamide gel electrophoresis results, ... | 1975 | 5398 |
| the modification of sulfhydryl groups of glutamine synthetase from bacillus stearothermophilus with 5, 5'-dithiobis(2-nitrobenzoic acid). | the sh groups of glutamine synthetase ec 6.3.1.2 from bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of sh groups in the molecule as well as the effect of the modification on the enzyme activity. three sh groups per subunit were detected after complete denaturation of the enzyme with 6 m urea, one of which was essential for the enzyme activity in view of its reactivity with 5, 5'-dithiobis(2-nitrobenzoic acid) on addition of ... | 1975 | 5420 |
| the primary structure of trna phe from bacillus stearothermophilus and its analogy with other trna's from different origins. | 1975 | 58588 | |
| isoleucyl-trna synthetase from bacillus stearothermophilus: specificity of the aminoacylation reaction for aminoacid and trna. | 1975 | 58626 | |
| evidence of the involvement of a 50s ribosomal protein in several active sites. | the functional role of the bacillus stearothermophilus 50s ribosomal protein b-l3 (probably homologous to the escherichia coli protein l2) was examined by chemical modification. the complex b-l3-23s rna was photooxidized in the presence of rose bengal and the modified protein incorporated by reconstitution into 50s ribosomal subunits containing all other unmodified components. particles containing photooxidized b-l3 are defective in several functional assays, including (1) poly(u)-directed poly( ... | 1975 | 52 |
| ligand binding and enzymic catalysis coupled through subunits in tyrosyl-trna synthetase. | the interaction of the tyrosyl-trna synthetase from bacillus stearothermophilus with its substrates in the aminoacyl adenylation reaction has been studied by stopped-flow fluorescence. the observed changes have been assigned to their chemical and physical processes by comparison with equilibrium dialysis, pyrophosphate exchange kinetics and rapid quenching and sampling techniques to give the rate constants for ligand binding, the formation of tyrosyl adenylate, and the reverse reaction. the stoi ... | 1975 | 162826 |
| the binding of nicotinamide-adenine dimucleotide to glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. | the binding of nad+ to glyceraldehyde 3-phosphate dehydrogenase (ec 1.2.1.12) from bacillus stearothermophilus has been studied by measurement of protein fluorescence quenching. slight negative co-operativity was observed in the binding of the third and fourth coenzyme molecules to the tetrameric enzyme. the first two coenzyme molecules were tightly bound. in this respect the enzyme resembles that from sturgeon muscle rather than that from yeast. | 1975 | 175789 |
| a chemical approach to the fine structure of biomolecular complexes: the amino terminal region of the 50s ribosomal "a" protein from bacillus stearothermophilus. | an experimental approach and methodology are described for determining the reactive properties and ionization constants of individual functional groups of proteins within biomolecular complexes. the ionization constants and reactivities of the methionyl-l amino terminus and the lysyl-3 residue of the alanine rich 50s ribosomal "a" protein from bacillus stearothermophilus have been determined by an extension of the competitive labeling technique used by h. kaplan, k. j. stevenson, and b. s. hartl ... | 1975 | 234242 |
| production and purification of the thermophilic bacteriophage tp-84. | a new procedure for production and purification of the thermophilic bacteriophage tp-84 in high yields is described. cultures of bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a ph of 6.5, were infected with the phage in a 100-liter fermentor. addition of magnesium chloride (0.01 m) and a temperature of 58-c were essential for maximal phage production. phage (5 times 1011 infective particles/ml) was precipitate ... | 1975 | 234714 |
| glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. | 1975 | 236448 | |
| glucose-6-phosphate isomerase from bacillus stearothermophilus. | 1975 | 236463 | |
| isolation and characterization of isocitrate lyase and malate synthase from bacillus stearothermophilus. | 1975 | 236948 | |
| pyruvate carboxylase from bacillus stearothermophilus: molecular size, biotin content and subunit constitution. | 1975 | 236949 | |
| mycotoxin bioassay, using bacillus stearothermophilus. | spores of bacillus stearothermophilus in standardized spore strips are pretreated with solutions of the mycotoxins aflatoxin b1, patulin, rubratoxin b, and diacetoxyscirpenol and subsequently incubated in a nutrient solution containing bromocresol purple as ph indicator. after 16.5 hr of inclubation the color of the indicator medium inoculated with untreated spore strips of b. stearothermophilus changes from purple to yellow; no color change occurs in the indicator medium inoculated with spore s ... | 1975 | 237876 |
| effect of ph on sporulation of bacillus stearothermophilus. | an improved broth medium was developed for high growth yields of bacillus subtilis var. niger ncib 8649, bacillus cereus ncib 9373, and bacillus stearothermophilus ncib 8919 and atcc 7953. sporulation was abundant (1.1 times 10-8 b. subtilis var. niger and 9.2 times 10-7 b. cereus per ml) at an initial ph of 7.0. sporulation of both strains of b. stearothermophilus took place (1.9 times 10-7 and 2.4 times 10-7/ml, respectively) in this medium when initial ph values of 7.7 to 8.7 were used. | 1975 | 238470 |
| synthesis of polyglycerol phosphate by bacillus stearothermophilus. | a particulate enzyme preparation from bacillus stearothermophilus synthesized 1,3-poly(glycerol phosphage) from cdpglycerol at an optimum ph of 8.0 and the reaction was stimulated by divalent cations. km for cdpglycerol was 0.18 mm. the synthesis was inhibited by cmp, cdp, and ctp and by concentrations of cdp-glycerol above 0.49 mm. the reaction was irreversible, the product had an average chain length of 8 glycerol units. about two thirds of the polymers were synthesized in entirety while the r ... | 1975 | 238960 |
| biochemical changes during sporulation of bacillus stearothermophilus. | the process of sporulation was studied in bacillus stearothermophilus. a medium is described that supports good growth and sporulation of the organism. in this medium, which contains glucose, salts, and amino acids, acetate starts to accumulate before any of the glucose is catabolized. enzymes of the tricarboxylic acid cycle are present at all times during growth and sporulation and are found in dormant spores. as the glucose in the culture is consumed, acetate rapidly increases and the ph of th ... | 1975 | 240496 |
| affinity chromatography on immobilised nucleotides. some applications to the purification of thermophilic dehydrogenases and kinases. | the effect of ph and temperature on the capacity and binding of bacillus stearothermophilus, alcohol dehydrogenase and phosphofructokinase to n6-(6-aminohexyl)-5'-amp-sepharose has been examined. specific elution from the substituted amp-sepharose was examined using a variety of cofactors, fragments of cofactors and substrates. a purification scheme for each enzyme on the substituted amp-sepharose using nucleotides and gradients of ph and salt is presented. interestingly, elevated temperature in ... | 1975 | 240692 |