Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| structures of two cell wall-associated polysaccharides of a streptococcus mitis biovar 1 strain. a unique teichoic acid-like polysaccharide and the group o antigen which is a c-polysaccharide in common with pneumococci. | the cell wall of streptococcus mitis biovar 1 strain sk137 contains the c-polysaccharide known as the common antigen of a closely related species streptococcus pneumoniae, and a teichoic acid-like polysaccharide with a unique structure. the two polysaccharides are different entities and could be partially separated by gel chromatography. the structures of the two polysaccharides were determined by chemical methods and by nmr spectroscopy. the teichoic acid-like polymer has a heptasaccharide phos ... | 2000 | 11106426 |
| the galu gene of streptococcus pneumoniae that codes for a udp-glucose pyrophosphorylase is highly polymorphic and suitable for molecular typing and phylogenetic studies. | the enzyme utp-glucose-1-phosphate uridylyltransferase (udp-glucose pyrophosphorylase, udpg:pp) is synthesized by practically all organisms, although prokaryotic udpg:pps are evolutionarily unrelated to the eukaryotic counterparts. the primary structure of prokaryotic udpg:pps is well conserved, although little information exists on the polymorphism of the genes coding for these enzymes. it has been reported that the galu gene encoding the streptococcus pneumoniae udpg:pp is absolutely required ... | 2000 | 11137293 |
| the use of immunoliposomes for specific delivery of antimicrobial agents to oral bacteria immobilized on polystyrene. | antibacterial immunoliposomes have been prepared using covalently bound antibody, raised to the cell surface of the bacterium streptococcus oralis (s. oralis), and incorporating the bactericides chlorhexidine and triclosan. a regrowth assay, in which the ability of a bacterial biofilm immobilised on polystyrene to grow after exposure to a test solution, was undertaken to study the action of the antibacterial immunoliposomes. the antibacterial anti-oralis immunoliposomes show enhanced growth inhi ... | 2000 | 11261879 |
| a comparative pilot study of the effects of a dentifrice containing green tea bioflavonoids, sanguinarine or triclosan on oral bacterial biofilm formation. | the aim of the present study was to evaluate the effect of a green tea bioflavonoid/zinc ascorbate-containing dentifrice (natural antioxidant toothpaste) on bacterial plaque biofilm accumulation. plaque biofilms were formed and observed on saliva-conditioned synthetic hydroxyapatite disks (ha) over 4 days. two test treatments were performed daily over a 4-day experimental period for a total of 8 treatments. four test dentifrices were evaluated: a 0.1% bioflavonoid/0.2% zinc ascorbate; a non-acti ... | 2000 | 11460284 |
| adhesion in vitro of oral streptococci to porcelain, composite resin cement and human enamel. | we investigated the initial adhesion in vitro of oral streptococci to porcelain inlays. four strains of streptococci, streptococcus mitis nctc12261, streptococcus oralis atcc9811, streptococcus sanguis atcc10556 and streptococcus sobrinus omz176, were used in this study. the disc specimens were made of porcelain, composite resin cement and human enamel. these specimens, with or without a saliva-coat, were immersed in a suspension of each streptococci strain at 37 degrees c for 1 hr, and the numb ... | 2000 | 11816366 |
| in-vitro activity of synercid and related drugs against streptococcus oralis isolated from septicaemia and endocarditis cases. | the increase in resistance to gram positive organisms and seriousness of infective endocarditis, makes it necessary to look for an alternate treatment. | 2000 | 24019702 |
| binding of oral streptococci to human fibrinogen. | the interaction of oral streptococci with human fibrinogen was investigated. streptococcus gordonii was chosen as a representative species to study the binding to fibrinogen. s. gordonii dl1 adhered to immobilized fibrinogen and bovine serum albumin. binding to immobilized fibrinogen was saturable, concentration and temperature dependent. the binding of s. gordonii dl1 to fibrinogen was inhibited by anti-fibrinogen antibody. heating of the bacteria for 1 h at 95 degrees c resulted in 90% inhibit ... | 2001 | 11240861 |
| altered antigenicity in periodontitis patients and decreased adhesion of porphyromonas gingivalis by environmental temperature stress. | periodontopathogenic bacteria survive various environmental changes during the progression of periodontal disease. alterations in metabolism and protein expression will have to take place to adapt their physiological functions to environmental stress. we examined the effects of an elevation of 2 degrees c in temperature on the adhesive ability and antigenicity of porphyromonas gingivalis. elevation of growth temperature of p. gingivalis from 37 degrees c to 39 degrees c remarkably suppressed the ... | 2001 | 11240867 |
| co-adhesion and removal of adhering bacteria from salivary pellicles by three different modes of brushing. | this study compares removal of pairs of co-adhering and non-co-adhering oral actinomyces and streptococci by hand, electric and sonic brushing from salivary pellicles. in addition, re-deposition of a co-adhering and non-co-adhering streptococcal strain to brushed pellicles was studied. first, actinomyces were allowed to adhere to a pellicle in a flow chamber, after which streptococci suspended in saliva were perfused through the chamber at 33 c. pellicles with adhering bacterial pairs were brush ... | 2001 | 11695753 |
| dysregulation of monocyte oxidative burst in streptococcal endocarditis. | streptococcal subacute endocarditis is characterized by low-grade systemic inflammation. although structural cardiac defects are pivotal, phagocytic cells, i.e. monocytes and neutrophils, are involved in the induction and the course of bacterial endocarditis. decreased production of reactive oxygen metabolites was described in long-lasting infections. we hypothesized that the oxidative burst of phagocytes induced by the infecting organism is defective in patients with streptococcal endocarditis. | 2001 | 11737229 |
| electric field induced desorption of bacteria from a conditioning film covered substratum. | desorption of three oral bacterial strains from a salivary conditioning film on an indium tin oxide electrode during application of a positive (bacterial adhesion to the anode) or a negative electric current was studied in a parallel plate flow chamber. bacterial adhesion was from a flowing suspension of high ionic strength, after which the bacterial suspension was replaced by a low ionic strength solution without bacteria and currents ranging from -800 to +800 microa were applied. streptococcus ... | 2001 | 11745167 |
| infection and mucosal injury in cancer treatment. | the oral and gastrointestinal mucosa acts as an important mechanical barrier that prevents local or systemic invasion by microorganisms. cytotoxic chemotherapy-induced mucosal injury (mi) of oral cavity and intestinal epithelium occurs in many patients treated for malignancy. compromise of the mucosal barrier can contribute to local invasion by colonizing microorganisms and, subsequently, to systemic infection. historically, gram-negative bacteremia has been the most problematic bacterial infect ... | 2001 | 11694563 |
| salivary concentrations of urea released from a chewing gum containing urea and how these affect the urea content of gel-stabilized plaques and their ph after exposure to sucrose. | the objectives were to: (1) determine the salivary concentrations of urea during 20 min chewing of a sugar-free gum containing 30 mg of urea; (2) measure the degree to which this urea would diffuse into a gel-stabilized plaque; (3) study the effect of the urea on the fall and subsequent rise in ph (stephan curve) on exposure to 10% sucrose for 1 min; (4) model the measurements 2 and 3 mathematically. in point 1, the salivary urea concentration of the 12 subjects peaked at 47 mmol/l in the first ... | 2001 | 11641570 |
| identification of the psaa gene, coding for pneumococcal surface adhesin a, in viridans group streptococci other than streptococcus pneumoniae. | the gene encoding the pneumococcal surface adhesin a (psaa) protein has been identified in three different viridans group streptococcal species. comparative studies of the psaa gene identified in different pneumococcal isolates by sequencing pcr products showed a high degree of conservation among these strains. psaa is encoded by an open reading frame of 930 bp. the analysis of this fragment in streptococcus mitis, streptococcus oralis, and streptococcus anginosus strains revealed a sequence ide ... | 2001 | 11527799 |
| tts, a processive beta-glucosyltransferase of streptococcus pneumoniae, directs the synthesis of the branched type 37 capsular polysaccharide in pneumococcus and other gram-positive species. | the type 37 capsule of streptococcus pneumoniae is a homopolysaccharide built up from repeating units of [beta-d-glcp-(1-->2)]-beta-d-glcp-(1-->3). the elements governing the expression of the tts gene, coding for the glucosyltransferase involved in the synthesis of the type 37 pneumococcal capsular polysaccharide, have been studied. primer extension analysis and functional tests demonstrated the presence of four new transcriptional start points upstream of the previously reported tts promoter ( ... | 2001 | 11264282 |
| nationwide german multicenter study on the prevalence of antibiotic resistance in streptococcal blood isolates from neutropenic patients and comparative in vitro activities of quinupristin-dalfopristin and eight other antimicrobials. | in a prospective multicenter study (1996 to 1999), 156 episodes of bacteremic streptococcal infections of neutropenic patients were evaluated. streptococcus oralis (26.3%), s. pneumoniae (26.3%), s. agalactiae (11.5%), s. mitis (9%), and s. pyogenes (5.8%) were the predominant species. four strains (2.6%) were found to be intermediately resistant to penicillin. one strain (0.6%) was found to be highly resistant to penicillin (mic, 8 mg/liter). reduced susceptibility to penicillin was detected am ... | 2001 | 11326015 |
| investigation of bacteremia after orthodontic banding and debanding following chlorhexidine mouth wash application. | this study investigates the prevalence of bacteremia after orthodontic banding and debanding, following the application of a 0.2% chlorhexidine gluconate mouthwash. the banding and debanding groups were each composed of 40 young adult patients. in the banding group, patients were asked to rinse their mouth with chlorhexidine gluconate for 60 seconds just prior to fitting of the bands. in the debanding group, they were asked to use the mouthwash immediately before removal of bands and brackets. i ... | 2001 | 11407771 |
| mosaic genes and mosaic chromosomes: intra- and interspecies genomic variation of streptococcus pneumoniae. | streptococcus pneumoniae remains a major causative agent of serious human diseases. the worldwide increase of antibiotic resistant strains revealed the importance of horizontal gene transfer in this pathogen, a scenario that results in the modulation of the species-specific gene pool. we investigated genomic variation in 20 s. pneumoniae isolates representing major antibiotic-resistant clones and 10 different capsular serotypes. variation was scored as decreased hybridization signals visualized ... | 2001 | 11254610 |
| utilization and acid production of beta-galactosyllactose by oral streptococci and human dental plaque. | beta-galactosyllactose is a trisaccharide containing the beta-galactosidic linkage at the nonreducing end. the purpose of this study was to determine whether certain oral streptococci could utilize four kinds of beta-galactosyllactoses. three of four beta-galactosyllactoses were unable to support growth of the oral streptococci and to be a substrate for producing acid from the cell suspensions and dental plaque. 4'-beta-galactosyllactose supported growth of streptococcus sanguis atcc 35105, atcc ... | 2001 | 11169141 |
| spatial arrangements and associative behavior of species in an in vitro oral biofilm model. | the spatial arrangements and associative behavior of actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis strains in an in vitro model of supragingival plaque were determined. using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distribution of the five strains were assessed during biofilm formation. the volume-derived cell numbers of each strain correlated w ... | 2001 | 11229930 |
| mannosidase production by viridans group streptococci. | the production of mannosidase activity by all currently recognized species of human viridans group streptococci was determined using an assay in which bacterial growth was dependent on the degradation of the high-mannose-type glycans of rnase b and subsequent utilization of released mannose. rnase b is an excellent substrate for the demonstration of mannosidase activity since it is a glycoprotein with a single glycosylation site which is occupied by high-mannose-type glycoforms containing five t ... | 2001 | 11230417 |
| validation of an in vitro biofilm model of supragingival plaque. | the study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. we describe a model of supragingival plaque containing actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to al ... | 2001 | 11269730 |
| comparative in-vitro activity of trovafloxacin and other related drugs against isolates of streptococcus oralis. | the in-vitro activity of trovafloxacin a new quinolone was compared with that of ciprofloxacin, erythromycin, various b-lactam and other appropriate antibiotics such as vancomycin, teicoplanin and clindamycin against 60 isolates of s. oralis. | 2001 | 11307110 |
| the effects of surface roughness and type of denture acrylic on biofilm formation by streptococcus oralis in a constant depth film fermentor. | to investigate the effects of surface roughness and type of denture acrylic on the early development of a streptococcus oralis biofilm in a constant depth film fermentor (cdff). | 2001 | 11442713 |
| analysis of bacterial detachment from substratum surfaces by the passage of air-liquid interfaces. | a theoretical analysis of the detachment of bacteria adhering to substratum surfaces upon the passage of an air-liquid interface is given, together with experimental results for bacterial detachment in the absence and presence of a conditioning film on different substratum surfaces. bacteria (streptococcus sobrinus hg1025, streptococcus oralis j22, actinomyces naeslundii t14v-j1, bacteroides fragilis 793e, and pseudomonas aeruginosa 974k) were first allowed to adhere to hydrophilic glass and hyd ... | 2001 | 11375160 |
| effects of chlorhexidine digluconate and hydrogen peroxide on porphyromonas gingivalis hemin binding and coaggregation with oral streptococci. | porphyromonas gingivalis, a gram-negative anaerobe, is one of the major causative agents of periodontal disease. in this study, the effects of chlorhexidine digluconate and hydrogen peroxide on the hemin binding of p. gingivalis and coaggregation of this bacterium with oral streptococci were examined. the pretreatment of p. gingivalis w50 and 381 with chlorhexidine digluconate and hydrogen peroxide increased the hemin binding of these bacteria. the hemin binding of p. gingivalis was increased by ... | 2001 | 11383630 |
| altered protein expression of streptococcus oralis cultured at low ph revealed by two-dimensional gel electrophoresis. | streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. to identify proteins that are differentially expressed by s. oralis growing under conditions of low ph, soluble cellular proteins extracted from bacteria grown in batch culture at ph 5.2 or 7.0 were analyzed by two-dimensional (2-d) gel electrophoresis. thirty-nine proteins had alter ... | 2001 | 11472910 |
| high proportion of pharyngeal carriers of commensal streptococci resistant to erythromycin in spanish adults. | the presence of erythromycin-resistant (err) commensal streptococci in the throat of 110 healthy subjects and 87 patients with pharyngitis was investigated. the resistance determinants were studied by pcr using the primers for mef and erm genes, followed by hybridization and sequencing analysis. overall, 94.4% of the subjects carried one or more err strains in their pharynx. a total of 253 err strains was studied: 127 (50.2%) showed constitutive or inducible resistance to clindamycin (mls(b) phe ... | 2001 | 11481292 |
| mutualism versus independence: strategies of mixed-species oral biofilms in vitro using saliva as the sole nutrient source. | during initial dental plaque formation, the ability of a species to grow when others cannot would be advantageous, and enhanced growth through interspecies and intergeneric cooperation could be critical. these characteristics were investigated in three coaggregating early colonizers of the tooth surface (streptococcus gordonii dl1, streptococcus oralis 34, and actinomyces naeslundii t14v). area coverage and cell cluster size measurements showed that attachment of a. naeslundii and of s. gordonii ... | 2001 | 11500457 |
| transfer of tn916-like elements in microcosm dental plaques. | microcosm dental plaques were grown from an inoculum of human saliva in a constant-depth film fermentor. the inoculum contained four tetracycline-resistant streptococcal species, each of which contained a tn916-like element. this element was shown to transfer to other streptococci both in filter-mating experiments and within the biofilms in the fermentor. | 2001 | 11557498 |
| pcr and sequencing of independent genetic targets for the diagnosis of culture negative bacterial endocarditis. | molecular methods utilizing broad-range primers for 16s rdna pcr and sequencing have been widely evaluated for their utility in culture negative diagnostic bacteriology. difficulties in determining the incidence of false positive pcr results, especially in the absence of an equally sensitive confirmatory method however, have prevented wide clinical use of this sensitive technology. here we report two cases of culture-negative endocarditis, in which pcr using 16s rdna broad-range primers generate ... | 2001 | 11576785 |
| phosphorylcholine-dependent cross-reactivity between dental plaque bacteria and oxidized low-density lipoproteins. | antibodies reactive with phosphorylcholine (pc) are ubiquitous in human sera, but the antigens stimulating their production and their function are not clear. previous studies have shown that a significant proportion of dental plaque bacteria contain pc as determined by reactivity with pc-specific mouse myeloma proteins and monoclonal antibodies. additionally, serum antibody concentrations of immunoglobulin (igg) g anti-pc are higher in sera of individuals who have experienced periodontal attachm ... | 2001 | 11598029 |
| cariogenic potential of lactosylfructoside as determined by acidogenicity of oral streptococci in vitro and human dental plaque in situ. | the cariogenic potential of lactosylfructoside [o-beta-d-galactopyranosyl-(1-->4)-o-alpha-d-glucopyranosyl-(1<-->2)-beta-d-fructofuranoside] was estimated by experiments on oral streptococci in vitro and human dental plaque in situ. lactosylfructoside was unable to support growth of the strains of streptococcus mutans and s. sobrinus used in this study. however, it was able to support growth of strains of s. sanguis, s. mitis and s. oralis. acid was produced rapidly by cell suspensions of s. ora ... | 2001 | 11641569 |
| the predominant microflora of nursing caries lesions. | the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ... | 2001 | 11799279 |
| low cerebrospinal fluid beta-amyloid 42 in patients with acute bacterial meningitis and normalization after treatment. | csf-a beta 42 may be a marker of alzheimer's disease (ad). a decreased level of csf-a beta 42 is consistently found in ad and has been suggested to be related to the deposition of amyloid plaques in the brain. however, low csf-a beta 42 levels have also been found in disorders devoid of plaques, for instance creutzfeldt-jakob disease. to examine if the level of a beta 42 in csf is related to inflammatory processes, we studied csf-a beta 42 levels in eight patients with acute purulent bacterial m ... | 2001 | 11698140 |
| clinical picture: bacterial translocation in neutropenic sepsis. | 2001 | 11741622 | |
| poking holes in pneumococci. | 2002 | 11827801 | |
| [clinical and bacteriological features of six cases with intracranial abscess in childhood]. | from october 1988 to march 2001, 5 patients with 6 episodes of intracranial abscesses were admitted to chiba-children's hospital. average age when they were admitted was 10 years and 1 month. initial clinical symptoms were fever in 5 cases, and headache in 1 case. it took 21 days from the appearance of the initial symptoms to diagnose the intracranial abscess. four out of 5 patients had underlying diseases that were prone to cause intracranial abscess. two patients of these were cyanotic congeni ... | 2002 | 11905002 |
| clinical and microbiological studies of periodontal disease in sjögren syndrome patients. | little is known about the periodontal status of patients with sjögren's syndrome (ss), a chronic inflammatory autoimmune disease characterized by xerophthalmia and xerostomia. the aim of the present study was to evaluate whether the periodontal status of ss patients, in terms of clinical and microbiological parameters, differs from systemically healthy age- and gender-matched controls. | 2002 | 11908470 |
| repressed respiration of oral streptococci grown in biofilms. | the respiratory activities of oral streptococci grown in biofilms were found to be markedly repressed compared with those of cells from aerobic culture, or for streptococcus mutans gs-5, even for those grown in static culture. respiration rates generally reflected levels of nadh oxidase activities in cell extracts. superoxide dismutase levels were somewhat reduced in biofilm cells. however, sensitivities to oxidative damage caused by h2o2, t-butylhydroperoxide, or 8-hydroxyquinoline were not gre ... | 2002 | 11910496 |
| effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms. | antimicrobial effects of fluoride in vivo remain contentious. previous studies suggested that 1 mm naf reduced acid production from glucose, and prevented the enrichment of bacteria associated with caries in a chemostat model. the present study examines the effects of a lower fluoride concentration (0.53 mm, 10 ppm naf) in both biofilm and planktonic microbial communities. nine oral species were grown at ph 7.0 and pulsed on 10 successive days with glucose; bacterial metabolism was allowed to re ... | 2002 | 12037363 |
| identification of viridans streptococcal species causing bacterial meningitis in adults in taiwan. | in order to determine the species responsible for viridans-group streptococcal meningitis in adults in taiwan, seven cases of culture-proven meningitis due to these organisms were studied. the cases were all identified at the chang gung memorial hospital-kaohsiung over a period of 3 years, and they accounted for 9% (7/78) of all cases of culture-proven bacterial meningitis occurring in adults during this period. of the seven viridans streptococci isolates recovered, streptococcus constellatus ac ... | 2002 | 12072926 |
| selection of dairy bacterial strains as probiotics for oral health. | the aim of the present study was to select bacterial strains with potential properties as oral probiotics, namely for the prevention of dental caries. we examined 23 dairy microorganisms, out of which we identified two streptococcus thermophilus and two lactcoccus lactis strains that were able to adhere to saliva-coated hydroxyapatite beads to the same extent as streptococcus sobrinus omz176. two of them, strep. thermophilus ncc1561 and lactoc. lactis ssp. lactis ncc2211, were further successful ... | 2002 | 12120707 |
| interference of nontypeable haemophilus influenzae and moraxella catarrhalis by streptococcus oralis in adenoid organ culture: a possible strategy for the treatment of the otitis-prone child. | the role of viridans group streptococci (streptococcus oralis) in the prevention of colonization with nontypeable haemophilus influenzae and moraxella catarrhalis was investigated in an adenoid organ culture system. the adenoids from 100 patients who were undergoing adenoidectomy for either hypertrophy or recurrent otitis media were used. streptococcus oralis parker uniformly inhibited colonization with nontypeable h. influenzae or m. catarrhalis over a 24-hour period of incubation in adenoid or ... | 2002 | 12184590 |
| groesl sequence determination, phylogenetic analysis, and species differentiation for viridans group streptococci. | the full-length sequences of the groesl genes (also known as cpn10/60) of streptococcus anginosus, streptococcus constellatus, streptococcus gordonii, and streptococcus sanguis and the near full-length sequence of the groesl genes of streptococcus intermedius, streptococcus bovis, streptococcus mitis, streptococcus mutans, streptococcus oralis, and streptococcus salivarius were determined. the lengths of the groes genes from the 10 species listed above ranged from 282 to 288 bp, and the full-len ... | 2002 | 12202549 |
| native valve endocarditis due to streptococcus vestibularis and streptococcus oralis. | viridans streptococci are the commonest cause of native valve infective endocarditis (ie). the taxonomy of this group is evolving allowing new disease associations to be made. streptococcus vestibularis is a recently described member of the viridans group, first isolated from the vestibular mucosa of the human oral cavity. it has rarely been associated with human disease. streptococcus oralis, another member of the viridans group resident in the human oral cavity is a well known cause of ie and ... | 2002 | 12217730 |
| [35-year-old patient with oligoarthritis, bacteremia and aortic insufficiency]. | 2002 | 12219689 | |
| increased proliferative response of peripheral blood mononuclear cells and t cells to streptococcus mutans and glucosyltransferase d antigens in the exacerbation stage of recurrent aphthous ulcerations. | cytotoxic t lymphocyte-induced lysis of virus-infected oral epithelial cells has been shown to be a cause of early ulcer formation in recurrent aphthous ulcerations (rau). to test whether bacteria and their associated antigens are involved in the disease process of rau, the proliferative response (pr) to different streptococcal species in peripheral blood mononuclear cells (pbmc) and t cells isolated from rau patients at the exacerbation stage was determined. | 2002 | 12440086 |
| [chronic recurrent multifocal osteomyelitis of the mandible. a case report]. | chronic recurrent multifocal osteomyelitis (crmo) is a disorder rarely localized to the lower jaw. case report: a fourteen-year-old boy complained of a swollen of his lower jaw. after a ct scan, a bone biopsy was performed and yielded s. oralis against which an adapted intravenous antibiotherapy was administered without efficacy. the absence of malignant process and the revelation of an other focus of fixation at the tc bone scan localized on humerus called to mind the diagnosis of crmo. conclus ... | 2002 | 12536107 |
| [construction of the pyruvate oxidase gene deficiency variant strain of streptococcus oralis]. | the purpose of this study is to construct a pyruvate oxidase gene deficiency variant strain of streptococcus oralis (s. oralis). | 2002 | 12593207 |
| the relationship between odontogenic bacteraemia and orthodontic treatment procedures. | the purpose of this research was to estimate the prevalence and intensity of bacteraemia associated with orthodontic treatment procedures. the four procedures investigated were: an upper alginate impression, separator placement, band placement, and adjustment of an archwire on a fixed appliance. eighty-one children undergoing general anaesthesia (ga) for dento-alveolar surgery related to their orthodontic treatment were randomly allocated to the impression or separator group. a further 61 childr ... | 2002 | 12143093 |
| varidans streptococci in the oral flora of the patients at risk for infective endocarditis: species and penicillin susceptibilities. | this study examined viridans streptococci (sv) in oral flora of patients at risk of infective endocarditis (ie) by obtaining 57 isolates from gum-tooth margin swabs of 3 groups of patients. penicillin minimal inhibitory concentrations (mics) were determined by e test. group 1 was from 30 patients with prosthetic heart valves who did not receive antimicrobial agents within 3 months prior to recruitment. group 2 consisted of 21 patients with known rheumatic heart diseases who regularly received pe ... | 2002 | 12296416 |
| influence of dentifrices and dietary components in saliva on wettability of pellicle-coated enamel in vitro and in vivo. | in vitro salivary pellicles were found to be less hydrophobic by water contact angles than clinically formed pellicles. in this study, water contact angles were measured on enamel coated with pellicles adsorbed from reconstituted human whole saliva (rhws) and after exposure to dentifrices or dietary components. in addition, adhesion of streptococcus oralis j22 to pellicles formed from rhws with minor amounts of milk added and after exposure to dentifrices was studied. exposure of rhws-pellicles ... | 2002 | 12507216 |
| mitral valve endocarditis caused by streptococcus oralis occurring after upper gastrointestinal endoscopy. | 2002 | 12190201 | |
| bacterial detachment from salivary conditioning films by dentifrice supernates. | this study compared the detachment by supernates of nine different dentifrices of four oral bacterial strains adhering to a salivary pellicle in a parallel plate flow chamber. ultra-thin bovine enamel slabs were coated for 1.5 h with human whole saliva. following buffer rinsing, a bacterial suspension of streptococcus oralis, streptococcus sanguis, streptococcus mutans or actinomyces naeslundii was perfused through the flow chamber at a shear rate of 30 s-1 for four hours, and the number of adhe ... | 2002 | 11507932 |
| effect of acidic ph on expression of surface-associated proteins of streptococcus oralis. | streptococcus oralis, a member of the mitis group of oral streptococci, is implicated in the pathogenesis of infective endocarditis and is the predominant aciduric non-mutans-group streptococcus in dental plaque. we undertook to identify the most abundant surface-associated proteins of s. oralis and to investigate changes in protein expression when the organism was grown under acidic culture conditions. surface-associated proteins were extracted from cells grown in batch culture, separated by tw ... | 2003 | 12957916 |
| the antibacterial properties of solid supported liposomes on streptococcus oralis biofilms. | a novel system for the delivery of drugs to bacterial biofilms has been developed. the system is based on the use of anionic and cationic liposomes as drug carriers adsorbed on the surface of zinc citrate particles. the adsorption process results in the formation of solid supported vesicles (ssvs) which aids the stabilisation of the liposomes. anionic liposomes have been prepared by incorporation of phosphatidylinositol (pi) into dipalmitoylphosphatidylcholine (dppc) liposomes and cationic lipos ... | 2003 | 12711168 |
| enthalpy of interaction between coaggregating and non-coaggregating oral bacterial pairs--a microcalorimetric study. | bacterial adhesion and coaggregation are involved in the development of oral biofilms, called dental plaque. although various techniques have already been used to study different aspects of these bacterial interactions, microcalorimetry has not yet been applied. this paper describes how isothermal reaction calorimetry can be employed to determine the enthalpy of coaggregation between two oral bacterial pairs. for most biological processes, the enthalpy tends to reach a minimum value, reflecting ... | 2003 | 14500015 |
| the acid-tolerant microbiota associated with plaque from initial caries and healthy tooth surfaces. | the intent of this study was to compare the inherent acid tolerance of bacteria in samples of dental plaque from tooth sites in subjects with and without initial caries. plaque was collected from approximal surfaces showing early enamel caries and from healthy tooth surfaces in the same subjects, as well as from enamel surfaces of caries-free individuals. in addition to plating on blood agar, the plaque samples were plated directly on non-selective solid agar medium buffered to ph 7.0, 6.0, 5.5, ... | 2003 | 14571116 |
| non-contact removal of coadhering and non-coadhering bacterial pairs from pellicle surfaces by sonic brushing and de novo adhesion. | coadhesion between oral microbial pairs is an established factor in the spatiotemporal development and prevalence of mixed-species communities in early dental plaque in vivo. this study compares removal and de novo adhesion of pairs of coadhering and non-coadhering oral actinomyces and streptococci by sonic brushing on salivary pellicles in a non-contact mode as a function of the distance between the brush and the pellicle surface in vitro. first, actinomycetes were adhered to a pellicle surface ... | 2003 | 14632680 |
| [endocarditis and splenic abscesses]. | 2003 | 12666319 | |
| in vitro activities of garenoxacin (bms-284756) against streptococcus pneumoniae, viridans group streptococci, and enterococcus faecalis compared to those of six other quinolones. | the activity of garenoxacin, a new quinolone, was determined in comparison with other quinolones against different strains of s. pneumoniae, viridans group streptococci (vgs), and enterococcus faecalis. strains were quinolone-susceptible clinical isolates and quinolone-resistant strains with defined mechanisms of resistance obtained from either clinical isolates or derivatives of s. pneumoniae r6. clinical quinolone-susceptible strains of s. pneumoniae, vgs and e. faecalis showed garenoxacin mic ... | 2003 | 14576115 |
| effect of the environment on genotypic diversity of actinomyces naeslundii and streptococcus oralis in the oral biofilm. | the genotypic diversity of actinomyces naeslundii genospecies 2 (424 isolates) and streptococcus oralis (446 isolates) strains isolated from two sound approximal sites in all subjects who were either caries active (seven subjects) or caries free (seven subjects) was investigated by using the repetitive extragenic palindromic pcr. the plaque from the caries-active subjects harbored significantly greater proportions of mutans streptococci and lactobacilli and a smaller proportion of a. naeslundii ... | 2003 | 14602602 |
| [dna sequence analysis for the promoter of pyruvate oxidase gene from streptococcus oralis]. | to elucidate the molecular structure of pyruvate oxidase gene promoter. | 2003 | 14650985 |
| quantitative evaluation of bacteria adherent to polyelectrolyte hema-based hydrogels. | the use of adhesive poly(hema)-based hydrogels is standard practice in dental restorative procedures. microorganisms, which potentially can cause oral pathologies, may colonize these polymers. in the present work, bacterial adhesion to polymers prepared with 2-hydroxyethyl methacrylate (hema) and to different molar ratios of 2-acrylamido-2-methylpropane-sulfonic acid (amps) and/or to 2-methacryloyloxyethyl-tri-methyl-ammonium chloride (metac) co-monomers were tested. a colorimetric assay system ... | 2003 | 14517857 |
| quantitative detection of moraxella catarrhalis in nasopharyngeal secretions by real-time pcr. | the recognition of moraxella catarrhalis as an important cause of respiratory tract infections has been protracted, mainly because it is a frequent commensal organism of the upper respiratory tract and the diagnostic sensitivity of blood or pleural fluid culture is low. given that the amount of m. catarrhalis bacteria in the upper respiratory tract may change during infection, quantification of these bacteria in nasopharyngeal secretions (npss) by real-time pcr may offer a suitable diagnostic ap ... | 2003 | 12682118 |
| classification of oral streptococci by two-dimensional gel electrophoresis with direct activity stain for glycosyltransferases. | thirty eight strains of oral streptococci were divided into six types by two-dimensional gel electrophoresis (2-de) followed by glycosyltransferase (glt) activity stain: type 1, streptococcus mutans; type 2, streptococcus rattus; type 3, streptococcus sobrinus and streptococcus downei; type 4, streptococcus cricetus; type 5, streptococcus salivarius; and type 6, streptococcus sanguis, streptococcus oralis and streptococcus gordonii. in types 1, 2 and 5, two glucosyltransferases synthesizing wate ... | 2003 | 12753469 |
| coaggregation-mediated interactions of streptococci and actinomyces detected in initial human dental plaque. | streptococci and actinomyces that initiate colonization of the tooth surface frequently coaggregate with each other as well as with other oral bacteria. these observations have led to the hypothesis that interbacterial adhesion influences spatiotemporal development of plaque. to assess the role of such interactions in oral biofilm formation in vivo, antibodies directed against bacterial surface components that mediate coaggregation interactions were used as direct immunofluorescent probes in con ... | 2003 | 12754239 |
| glucan-binding proteins of the oral streptococci. | the synthesis of extracellular glucan is an integral component of the sucrose-dependent colonization of tooth surfaces by species of the mutans streptococci. in investigators' attempts to understand the mechanisms of plaque biofilm development, several glucan-binding proteins (gbps) have been discovered. some of these, the glucosyltransferases, catalyze the synthesis of glucan, whereas others, designated only as glucan-binding proteins, have affinities for different forms of glucan and contribut ... | 2003 | 12764072 |
| linezolid resistance since 2001: sentry antimicrobial surveillance program. | the oxazolidinone class of antimicrobials has demonstrated remarkable activity against gram-positive cocci. linezolid has proven to be a first-line therapeutic option for vancomycin-resistant strains. linezolid clinical trial results and subsequent published case reports cite rare resistance emerging in patients receiving prolonged therapy. | 2003 | 12773059 |
| subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult sudanese population using miswak or toothbrush regularly. | little information is available on the effect of miswak use on gingival microbiota. we assessed levels of 28 oral bacteria in subgingival plaque of adult sudanese miswak (n = 38) and toothbrush users (n = 36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. a pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. whole genomic dna p ... | 2003 | 12790510 |
| molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. | chronic maxillary sinusitis is a chronic inflammatory condition in which the role of microbial infection remains undefined. bacteria have been isolated from chronically inflamed sinuses; however, their role in the chronicity of inflammation is unknown. the objective of this study was to determine whether bacteria are present in clinical samples from chronic maxillary sinusitis and to assess the diversity of the flora present. washes and/or tissue samples from endoscopic sinus surgery on 11 patie ... | 2003 | 12808082 |
| viridans group streptococci are donors in horizontal transfer of topoisomerase iv genes to streptococcus pneumoniae. | a total of 46 ciprofloxacin-resistant (cip(r)) streptococcus pneumoniae strains were isolated from 1991 to 2001 at the hospital of bellvitge. five of these strains showed unexpectedly high rates of nucleotide variations in the quinolone resistance-determining regions (qrdrs) of their parc, pare, and gyra genes. the nucleotide sequence of the full-length parc, pare, and gyra genes of one of these isolates revealed a mosaic structure compatible with an interspecific recombination origin. southern ... | 2003 | 12821449 |
| [oral streptococci: factors and mechanisms of pathogenicity (part ii)]. | 2003 | 15341321 | |
| [oral streptococci: implications in infectious pathology]. | 2003 | 15341322 | |
| amino acid sequence requirements in the hinge of human immunoglobulin a1 (iga1) for cleavage by streptococcal iga1 proteases. | the amino acid sequence requirements in the hinge of human immunoglobulin a1 (iga1) for cleavage by iga1 proteases of different species of streptococcus were investigated. recombinant iga1 antibodies were generated with point mutations at proline 227 and threonine 228, the residues lying on either side of the peptide bond at which all streptococcal iga1 proteases cleave wild-type human iga1. the amino acid substitutions produced no major effect upon the structure of the mutant iga1 antibodies or ... | 2003 | 12595464 |
| septic discitis as a complication of infective endocarditis caused by streptococcus oralis. | 2003 | 12610830 | |
| mass transport of macromolecules within an in vitro model of supragingival plaque. | the aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. polyspecies biofilms containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, streptococcus sobrinus, veillonella dispar, and candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. subsequent examinatio ... | 2003 | 12620862 |
| prevalence of csh-like fibrillar surface proteins among mitis group oral streptococci. | the prevalence of csh-like fibrillar surface proteins among oral streptococci was investigated by elisa and by immunoelectron microscopy using antiserum raised to recombinant fragments of csha of streptococcus gordonii dl1. the majority of s. gordonii, streptococcus sanguis and streptococcus oralis strains tested elaborated short (ca. 50-80 nm long) surface fibrils and reacted with antiserum to the amino acid repeat region of csha, demonstrating the widespread nature of csh-like proteins among t ... | 2003 | 12654102 |
| gingivitis and toothbrushes: potential roles in viridans streptococcal bacteraemia. | we report a case of streptococcus oralis bacteraemia in a paediatric neutropenic patient with acute myeloid leukaemia whose predominant form of oral compromise was severe gingivitis, rather than mucositis. by phenotypic and genotypic analyses, the strain of s. oralis from blood culture was indistinguishable from an isolate from his mouth, suggesting that gingivitis may have provided a portal of entry for viridans streptococci into the bloodstream. to improve the patient's oral and dental hygiene ... | 2003 | 12504614 |
| gram-positive mera gene in gram-negative oral and urine bacteria. | clinical mercury resistant (hg(r)) gram-negative bacteria carrying gram-positive mercury reductase (mera)-like genes were characterized using dna-dna hybridization, pcr and sequencing. a pcr assay was developed which discriminated between the mera genes related to staphylococcus and those related to the bacillus/streptococcus mera genes by the difference in size of the pcr product. dna sequence analysis correlated with the pcr assay. the mera genes from acinetobacter junii, enterobacter cloacae ... | 2004 | 15358427 |
| denaturing high-performance liquid chromatography detection of ribosomal mutations conferring macrolide resistance in gram-positive cocci. | mutations in genes coding for l4 (rpld) or l22 (rplv) ribosomal proteins or in 23s rrna (rrl gene) are reported as a cause of macrolide resistance in streptococci and staphylococci. this study was aimed at evaluating a denaturing high-performance liquid chromatography (dhplc) technique as a rapid mutation screening method. portions of these genes were amplified by pcr from total dna of 48 strains of streptococcus pneumoniae (n = 22), staphylococcus aureus (n = 16), streptococcus pyogenes (n = 6) ... | 2004 | 14693554 |
| multiplex fish analysis of a six-species bacterial biofilm. | established procedures use different and seemingly incompatible experimental protocols for fluorescent in situ hybridization (fish) with gram-negative and gram-positive bacteria. the aim of this study was to develop a procedure, based on fish and confocal laser scanning microscopy (clsm), for the analysis of the spatial organization of in vitro biofilms containing both gram-negative and gram-positive oral bacteria. biofilms composed of the six oral species actinomyces naeslundii, candida albican ... | 2004 | 14706749 |
| a new in vivo interdental sampling method comparing a daily flossing regime versus a manual brush control. | the impact of flossing the interdental space is typically assessed by visual methods of questionable reliability, such as clinician observations and evaluations for scoring of plaque on visual tooth surfaces. the objective of this study was to develop and validate a method to measure interproximal quantities of dental plaque, thereby permitting quantitative evaluations of between-teeth cleaning and biofilm removal. | 2004 | 15688960 |
| oral streptococcal glyceraldehyde-3-phosphate dehydrogenase mediates interaction with porphyromonas gingivalis fimbriae. | interaction of porphyromonas gingivalis with plaque-forming bacteria is necessary for its colonization in periodontal pockets. participation of streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase (gapdh) and p. gingivalis fimbriae in this interaction has been reported. in this investigation, the contribution of various oral streptococcal gapdhs to interaction with p. gingivalis fimbriae was examined. streptococcal cell surface gapdh activity was measured by incubation of a constant num ... | 2004 | 15488735 |
| characterisation of viridans group streptococci with different levels of tet(m)-mediated tetracycline resistance. | streptococcus oralis 264-3, streptococcus mitis 254-1 and s. mitis 264-1, isolated from the oral cavities of two children were each found to carry the tet(m) gene but exhibited different degrees of reduced susceptibility to tetracycline (tetracycline mics of 2, 8 and 64 mg/l, respectively). the aim of this study was to determine the molecular basis for the different levels of tetracycline resistance (tc(r)) observed. escherichia coli hb101 carrying the cloned tet(m) genes exhibited similar level ... | 2004 | 15519474 |
| selection for high-level resistance by chronic triclosan exposure is not universal. | to investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. | 2004 | 15117935 |
| in vitro modeling of dental water line contamination and decontamination. | the contamination of dental unit water lines (duwl) is an emerging concern in dentistry. the aim of this study was to use an in vitro duwl to model microbial contamination and evaluate the decontamination efficacy of tetraacetylethylenediamine (taed) solutions. a duwl biofilm model used to simulate clinical conditions was used to generate a range of biofilms in duwl. three distinct biofilms were generated: (1) biofilm from water, (2) biofilm from a mix of water + contaminating human commensal ba ... | 2004 | 15183886 |
| [bacterial contamination of bony particles from the bone collection trap]. | intraoral bony defects can be filled with bony particles that are collected in a titanium filter while drilling. the rinsing liquid is contaminated with blood and saliva which implies that the bony particles are also contaminated with bacteria. the aim of this study was to determine quantitatively and qualitatively the degree of this contamination. over a period of three months bony particles were collected from 50 patients undergoing surgery. the bony particles were scraped off the filter, resu ... | 2004 | 15185482 |
| mercuric resistance genes in gram-positive oral bacteria. | mercury-resistant bacteria isolated from the oral cavities of children carried one of two types of mera gene that appear to have evolved from a common ancestor. streptococcus oralis, streptococcus mitis and a few other species had mera genes that were very similar to mera of bacillus cereus strain rc607. unlike the b. cereus rc607 mera gene, however, the streptococcal mera genes were not carried on tn5084-like transposons. instead, comparisons with microbial genomic sequences suggest the mera ge ... | 2004 | 15251199 |
| characterization of binding of streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase to porphyromonas gingivalis major fimbriae. | binding of streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase (gapdh) to porphyromonas gingivalis fimbriae was characterized via a biomolecular interaction analysis system. the interaction was specific, and the association constant value was 4.34 x 10(7) m(-1), suggesting that s. oralis gapdh functions as a dominant receptor for p. gingivalis and contributes to p. gingivalis colonization. | 2004 | 15322048 |
| autolysin-targeted lightcycler assay including internal process control for detection of streptococcus pneumoniae dna in clinical samples. | the development and clinical evaluation of a lightcycler pcr assay, including an internal process control (ipc), to detect the streptococcus pneumoniae autolysin gene in clinical samples is reported. the assay was developed to provide a second target for use in conjunction with existing pneumolysin pcr assays to increase the reliability of non-culture pcr diagnosis of pneumococcal infection. primers amplify a 173 bp fragment of the autolysin gene (lyta), which is detected by fluorescence-labelle ... | 2004 | 14970243 |
| glyceraldehyde-3-phosphate dehydrogenase of streptococcus oralis functions as a coadhesin for porphyromonas gingivalis major fimbriae. | cohesive interactions between porphyromonas gingivalis and plaque-forming bacteria, such as streptococcus oralis, are considered to play an important role in the colonization of p. gingivalis in periodontal sites. although p. gingivalis fimbriae have been reported to mediate coaggregation with s. oralis, the s. oralis molecule involved has not been identified. we identified the coadhesin of s. oralis atcc 9811 and purified it by affinity column chromatography. we found that the molecular mass of ... | 2004 | 14977937 |
| in vitro streptococcal adherence on prosthetic and implant materials. interactions with physicochemical surface properties. | the aim of this study was to evaluate streptococcal adherence to eight currently used prosthetic and implant materials, and enamel samples, after a salivary coating and to investigate the influence of substrata surface free energy (sfe) and its polar and non-polar components, as well as bacterial surface characteristics, on bacterial adherence. our results indicate a moderate hydrophobic character of saliva-coated surfaces and a pronounced basic character of the polar component of sfe values, ex ... | 2004 | 15009598 |
| pcr detection and identification of oral streptococci in saliva samples using gtf genes. | oral streptococci are major constituents of dental plaque, and their prevalence is implicated in various pathologies. therefore, accurate identification of oral streptococci would be valuable for studies of cariogenic plaque and for diagnostic use in infective endocarditis. many oral streptococci possess glucosyltransferase enzymes that synthesize glucan, which is an obligate component of dental plaque. we established a rapid and precise method to identify oral streptococci by pcr using the spec ... | 2004 | 15023429 |
| potential utility of a peptide deformylase inhibitor (nvp pdf-713) against oxazolidinone-resistant or streptogramin-resistant gram-positive organism isolates. | to evaluate the potency of a novel peptide deformylase inhibitor, nvp pdf-713, against gram-positive organisms having resistances to linezolid or quinupristin/dalfopristin. | 2004 | 15056649 |
| unique variations of pbp2b sequences in penicillin-nonsusceptible streptococcus pneumoniae isolates from korea. | pbp2b gene alterations were analyzed in 102 clinical isolates of streptococcus pneumoniae (30 penicillin susceptible, 23 intermediate, and 49 resistant) from korea. on the basis of pbp2b amino acid sequences, penicillin-nonsusceptible isolates of s. pneumoniae belonged to six groups, and 76% of the isolates in groups i to iv showed the same divergent block of amino acid alterations. thirteen isolates (group ii) also possessed a divergent block that was identical to that of streptococcus oralis. ... | 2004 | 15071038 |
| efficacy of garenoxacin in treatment of experimental endocarditis due to staphylococcus aureus or viridans group streptococci. | the activity of garenoxacin was investigated in rats with experimental endocarditis due to staphylococci and viridans group streptococci (vgs). the staphylococci tested comprised one ciprofloxacin-susceptible and methicillin-susceptible staphylococcus aureus (mssa) isolate (isolate 1112), one ciprofloxacin-susceptible but methicillin-resistant s. aureus (mrsa) isolate (isolate p8), and one ciprofloxacin-resistant mutant (grla) of p8 (isolate p8-4). the vgs tested comprised one penicillin-suscept ... | 2004 | 14693523 |
| influence of weight on removal of co-adhering bacteria from salivary pellicles by different modes of brushing. | this study compared removal of pairs of co-adhering and non-co-adhering oral actinomyces and streptococci from salivary pellicles by manual, rotating/oscillating electric and sonic toothbrushes, applying weights up to 240 g. first, actinomyces were allowed to adhere to a pellicle in a parallel plate flow chamber, after which streptococci suspended in saliva were perfused through the chamber at 33 degrees c. on average, 34-39% of the adhering bacteria were adhering as single organisms. for co-adh ... | 2004 | 14767163 |
| the influence of biosurfactants released by s. mitis bms on the adhesion of pioneer strains and cariogenic bacteria. | the influence of streptococcus mitis bms biosurfactants on the adhesion of eight pioneer and four cariogenic oral bacterial strains was, for a first screening, examined in a microtiter plate assay. the adhesion to pellicle-coated wells of three cariogenic strains was inhibited >70% by the biosurfactants, while only one pioneer strain showed >70% reduction. the reduction for the other strains did not exceed 50%. subsequently, adhesion of streptococcus mutans atcc 25175 and streptococcus sobrinus ... | 2004 | 15788225 |