Publications

resistance to extinction after schedules of partial delay or partial reinforcement in rats with hippocampal lesions.	two experimental procedures were employed to establish the reason why hippocampal lesions apparently block the development of tolerance for aversive events in partial reinforcement experiments, but do not do so in partial punishment experiments. rats were trained to run in a straight alley following hippocampal lesions (hc), cortical control lesions (cc) or sham operations (so), and resistance to extinction was assessed following differing acquisition conditions. in experiment 1 a 4-8 min inter- ...	1985	4029302
amikacin therapy for pseudomonas cellulitis in an amazon parrot.	amikacin sulfate, an aminoglycoside antibiotic, was used successfully to treat a case of severe pseudomonas cellulitus in an amazon parrot. side effects of polyuria and polydipsia occurred, but resolved without treatment 3 weeks after the antibiotic regimen. amikacin sulfate was given at a dosage of 0.04 mg/g of body weight, im, twice a day, based on antimicrobial sensitivity results. amikacin sulfate is indicated for p aeruginosa cellulitis that is resistant to gentamicin sulfate. although it i ...	1985	4030478
interactions of nuclear proteins with dna, during sperm differentiation in the ram.	ram spermatid nuclei and caput epididymal sperm nuclei were prepared and treated with dtt under conditions avoiding proteolysis. whole-mount preparations for the electron microscope were made in the presence or absence of the detergent joy. the chromatin of the less mature, non-round spermatid nuclei displayed a nucleosomal organization that gradually disappeared at the time the histones leave the nuclei (elongating spermatids). digestion with micrococcal nuclease suggests that polynucleosome ar ...	1985	4042771
hyperbaric pressure of 51 atmospheres is without effect on the depression of oxygen uptake in kidney tissue culture produced by halothane.	although anesthetized animals are awakened when subjected to increased pressure, compression does not result in antagonism of all phenomena associated with these drugs. it has recently been demonstrated that halothane's inhibition of respiration of isolated rat liver mitochondria is not reversed by hydraulic compression to 51 atmospheres. in order to determine whether this phenomenon can be extrapolated to the whole cell, we have investigated the effect of hydraulic compression of intact renal c ...	1985	4046731
free radical species mediating the toxicity of ozone for cultured rat lung fibroblasts.	we have reported a model system with which to study factors influencing the toxicity of o3 for cultured rat lung cells. this continuing investigation was to determine the relative contributions of free radical species to the toxicity of o3 as measured by 51cr release and the use of free radical scavengers. toxicity from modes of o3 exposure favoring stable free radicals (stationary cultures or added o3-exposed medium) was prevented by the h2o2 scavenger, catalase. toxicity produced by exposing c ...	1985	4049433
[cell electrophoretic studies of cattle lymphocytes].		1985	4051667
interaction of ultraviolet laser light with the cornea.	the effect of pulsed ultraviolet (uv) laser light on the cornea depends on wavelength (photon energy), irradiance (photon flux), and pulse firing rate. at the available excimer laser wavelengths of 193, 249, 308, and 351 nanometers, the authors have varied the irradiance per pulse (10 to 2000 mj/cm2) as well a pulse frequency (1, 10, 25 hz) and determined the thresholds for coagulation and ablation of the corneal stroma. the latter ablative action creates a groove resembling an incision and was ...	1985	4055287
short-term, mixed-diet overfeeding in man: no evidence for "luxuskonsumption".	after 13 days of weight maintenance diet (13,720 +/- 620 kj/day, 40% fat, 15% protein, and 45% carbohydrate), five young men (71.3 +/- 7.1 kg, 181 +/- 8 cm; means +/- sd) were overfed for 9 days at 1.6 times their maintenance requirements (i.e., +8,010 kj/day). twenty-four-hour energy expenditure (24-h ee) and basal metabolic rate (bmr) were measured on three occasions, once after 10 days on the weight-maintenance diet and after 2 and 9 days of overfeeding. physical activity was monitored throug ...	1985	4061637
the suicide inactivation of ox liver short-chain acyl-coa dehydrogenase by propionyl-coa. formation of an fad adduct.	propionyl-coa gave an unexpectedly low turnover (0.03 s-1) and high km (153 microm) as a substrate for ox liver short-chain acyl-coa dehydrogenase (scad). on addition of an excess of propionyl-coa to scad the flavin a448 decreased to about 30% of its original value and the peak at 368 nm was replaced by one at 335 nm. the decrease in a448 exhibited first-order kinetics and correlated with a first-order decrease in the enzyme's catalytic activity to 22% of the initial value. the flavin, released ...	1985	4062874
pteridine formation during lectin-induced lymphocyte activation.	after iodine oxidation, biopterin, 6-hydroxymethylpterin, and 6-formylpterin were identified in mouse spleen lymphocytes by means of reverse-phase hplc, crithidia assay, and oxidative degradation. concanavalin a activation induces a 30-fold increase in the pteridine amounts; biopterin as well as the sum of the carbinol and the aldehyde attain levels of 6-8 x 10(-12) mol/10(6) cells. the most rapid increase occurs during the first 24 hr. thus, pteridine accumulation precedes the period of lymphoc ...	1985	4066775
[ultrastructural characteristics of the salivary glands of of the tick ixodes persulcatus. ii. type iii alveoli of the sucking female].	electron microscope observations were conducted to study changes in the cellular structure of iiid type alveoli of feeding females of i. persulcatus. within the first 1-2 days of feeding granulosecreting glandular cells "d" and "e" get free of secretory inclusions. after that cells "d" degenerate completely and cells "e" undergo morphological transformations and start to fulfil osmoregulatory functions. from the third day of feeding cytoplasm of cells "e" and basal intersticial cells form a comp ...	1985	4069743
[drug susceptibility of staphylococci isolated from clinical specimens].	eighty-eight strains of staphylococci were isolated from a variety of specimens collected from patients at our university hospital from june to august 1982. these strains were identified as staphylococcus aureus (86%), staphylococcus epidermidis (6.8%), staphylococcus haemolyticus (2.3%), staphylococcus capitis (2.3%), and staphylococcus simulans (1.1%). one strain could not be identified with certainty as a currently recognized species. a majority (70%) of the s. aureus strains was resistant to ...	1985	4089343
interferon in experimental autoimmune encephalomyelitis (eae): effects of exogeneous interferon on the antigen-enhanced adoptive transfer of eae.	the transfer of experimental autoimmune encephalomyelitis (eae) can be enhanced by incubating lymph node cells (lnc) from eae rats with myelin basic protein (mbp). addition of rat fibroblast interferon (rfifn) to cultures of eae-lnc just before the addition of mbp, resulted in the inhibition of the adoptive transfer of eae. both development of clinical signs, such as weight loss and hind limb paralysis, and histopathological lesions, lymphocytic perivascular infiltrates of the central nervous sy ...	1985	2579912
nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section.	the sciatic nerve was sectioned unilaterally in rats and nerve growth factor (ngf) applied locally to the nerve stump for the following 10-14 days using an indwelling osmotic pump. the aim of the experiment was to test whether ngf had any effect on the previously reported neurophysiological and neurochemical events that occur central to a peripheral nerve lesion. the method of application allowed the sciatic nerve on the other side to be used as a control. primary afferent depolarization fell, a ...	1985	2581648
mechanisms of resistance to chemicals in arthropod parasites of veterinary importance.	this review evaluates the significance, for ectoparasites, of the four resistance mechanisms that have emerged in arthropods as defence against chemicals used for their control. in particular the contrast of the relative importance of each mechanism in each of the major ectoparasitic pests of economic importance (sheep blowflies, mosquitoes and ticks) is discussed. use is made of the experience gained in attempting to overcome these mechanisms to assess the consequences of resistance, and the ch ...	1985	2863888
peroxisomal oxidases and suggestions for the mechanism of action of insulin and other hormones.		1985	2863924
[antiprogestational action of 5 alpha-dihydronorethisterone].		1985	2934946
maternal aging as the important etiological factor in human aneuploidy.		1985	2937399
recognition of staphylococcus saprophyticus in urine cultures by screening colonies for production of phosphatase.	phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. phosphatase-negative staphylococci, such as staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as staphylococcus epidermidis, by spot testing colonies on filter paper saturated with 1 n naoh. phosphatase-positive colonies turned pink within seconds, and phosphatase-negative colonies showed no color. none of 55 s. saprophyticus iso ...	1985	2984240
(taa)n within sequences flanking several intrachromosomal variant surface glycoprotein genes in trypanosoma brucei.	in trypanosoma brucei telomeric copies of intrachromosomal variant surface glycoprotein (vsg) genes are produced through a gene conversion mechanism and are expressed if the telomere is or becomes transcriptionally activated. we have analyzed a sequence that occurs 1 to 1.5 kb 5' to several intrachromosomal vsg genes. this flanking sequence has three distinct sections: a 5' section containing 5 to 116 taa or taa-like tandem repeats; a moderately conserved sequence with an alternating gt characte ...	1985	2987874
[hereditary sclerodactyly and syndactyly].	a 61-year-old man is described with sclerodactyly of the hands and syndactyly of the second and third toes. hereditary sclerodactyly is a rare condition, beginning in early youth with flexion contracture of the fingers. in this patient the skin of the fingers was sclerotic and thickened, and the dorsal skin of the hands was atrophic and dry. the condition did not progress nor did it show signs of raynaud's phenomenon. both feet showed syndactyly of the second and third toes. the family tree sugg ...	1985	2989220
reactivation of latent murine cytomegalovirus from kidney.	cytomegalovirus (cmv) is presumed to cause latent infection, but the sites of infection are incompletely known. we propose that latent murine cytomegalovirus is present in kidney and may be reactivated by explanation. immunosuppressive agents and allogeneic stimuli may enhance this process. balb/c mice were infected 11 to 14 months previously with 10(5) pfu of smith strain murine cytomegalovirus intraperitoneally. kidneys from 15 infected and nine uninfected mice were washed, minced into 1 to 2 ...	1985	3003444
a new partition thin-layer chromatographic method for steroid separations.	tlc plates with a 25 mu thick polyamide stationary phase were modified for the separation of neutral steroids by impregnation with propylene glycol. a mixture of tritiated 5 alpha-androstan-3 alpha,17 beta-diol, testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one and 4-androstene-3,17-dione was applied to the plate and developed in a toluene mobile phase to a height of 13.6 cm. this resulted in complete resolution of the 4 compounds as detected by a gas flow scanner or imaging analyzer. cuttin ...	1985	3835732
powerline frequency electric and magnetic fields: a pilot study of risk perception.	the perception of the potential risk arising from human exposure to 50/60 hz electric and magnetic fields was studied with a quasi-random sample of 116 well-educated, opinion leaders using the risk perception framework previously developed by slovic, fischhoff, and lichtenstein. these individuals rated exposure to fields from transmission lines and electric blankets on a variety of scales that have been found useful in characterizing people's risk attitudes and perceptions. these judgments allow ...	1985	3843677
[candida albicans endophthalmitis in a heroin addict].		1985	3879749
[analysis of cell surface antigens of gastric cancer and preliminary study of clinical application by using mouse monoclonal antibodies produced against nugc3].	four monoclonal antibodies (gc301, 302, 303, 304) produced by hybridomas obtained from mice immunized with nugc3 were analyzed by serological assay (mixed hemadsorption assay-mha) and immunoperoxidase technique. gc301(igg1) showed tumor restricted reactivity in serological analysis, but it reacted with fibrous interstitial tissues immunohistochemically. gc302(igg1) was serologically reactive with epithelial tumors such as gastric cancers, colon cancers and gynecological cancers, but not with bra ...	1985	3889584
quantitative analysis of in situ hybridization methods for the detection of actin gene expression.	we have implemented an efficient, quantitative approach for the optimization of in situ hybridization using double-stranded recombinant dna probes. the model system studied was actin mrna expression in chicken embryonic muscle cultures. actin and control (pbr322) probes were nick-translated with p32 labeled nucleotides, hybridized to cells grown on coverslips, and quantitated in a scintillation counter. cellular rna retention was monitored via the incorporation of h3-uridine into rna prior to ce ...	1985	3889842
quantitative analysis of in situ hybridization methods for the detection of actin gene expression.	we have implemented an efficient, quantitative approach for the optimization of in situ hybridization using double-stranded recombinant dna probes. the model system studied was actin mrna expression in chicken embryonic muscle cultures. actin and control (pbr322) probes were nick-translated with p32 labeled nucleotides, hybridized to cells grown on coverslips, and quantitated in a scintillation counter. cellular rna retention was monitored via the incorporation of h3-uridine into rna prior to ce ...	1985	3889842
[bacterial contamination of pump-collected breast milk].	breast feeding of premature, new-born babies is often difficult when they become sick and are transferred to a children's hospital. the mother's milk should be collected and provided to the child without a loss of quality or too high a bacterial count which could endanger his health. we collected 113 samples of milk using electric vacuum breast pumps and then determined the bacterial contamination. in most cases there was evidence of bacteria normally found on the skin although more than 10(5)/m ...	1985	3900703
[serology of mediterranean boutonneuse fever. kinetics of antibodies detected by 3 methods: indirect immunofluorescence, indirect hemagglutination and latex agglutination].	the purpose of this work was to determine the kinetics of antibodies in mediterranean spotted fever as determined by three serologic methods: indirect fluorescent antibody test, latex agglutination test and indirect hemagglutination test. no difference was noticed in the early kinetics but after 6 months, the latex agglutination test and the indirect hemagglutination test did not detect antibodies but the indirect fluorescent antibody test was still positive. this reaction is convenient for sero ...	1985	3903623
plasmid-mediated resistance to lincomycin by inactivation in staphylococcus haemolyticus.	staphylococcus haemolyticus bm4610 was resistant to high levels of lincomycin and susceptible to macrolides, clindamycin, and streptogramins. this resistance phenotype, not previously reported for a human clinical isolate, was due to inactivation of the antibiotic. the gene conferring resistance to lincomycin in strain bm4610 was carried by a 2.5-kilobase plasmid, pip855, which was cloned in escherichia coli. plasmid pip855 caused inactivation of both lincomycin and clindamycin in s. haemolyticu ...	1985	3907492
the sfia11 mutation prevents filamentation in a response to cell wall damage only in a reca+ genetic background.	n-alpha-palmitoyl-l-lysyl-l-lysine dihydrochloride ethyl ester (pll) at sublethal doses causes filamentous growth of e. coli strains except sfia mutants, which divide normally in its presence. pll does not elicit the sos responses as judged by lambda prophage induction, an increase of reca protein synthesis or induction of the sfia operon in a sfia::lacz fusion strain. thus, it appears that filamentation caused by pll is not an sos function and might be the result of membrane damage by pll, whic ...	1985	3911030
human pancreatic growth hormone (gh)-releasing hormone stimulates gh synthesis and release in infant rats. an in vivo study.	administration of human pancreatic gh-releasing factor 1-40 (hpgrf-40) at doses of 1, 10, 20, 100, and 500 ng/100 g bw sc induced in 10-day-old rats a clear-cut rise in plasma gh 15-min post-injection, although the effect was not dose-related and peak gh levels were already present after the lowest grf dose. in 25-day-old rats, hpgrf induced only a slight rise in plasma gh at the dose of 500 ng/100 g bw sc, whereas it was completely ineffective at the lower doses. in 5-day-old rats, hpgrf (20 ng ...	1985	3917905
comparison of bacteriostatic and bactericidal inhibitors of neisseria gonorrhoeae growth produced in vitro by urogenital staphylococci.	coagulase-negative staphylococci are known to produce bactericidal and bacteriostatic antigonococcal activities. out of 12 staphylococci, staphylococcus haemolyticus (four isolates) was identified as the principal source of bactericidal activity, whereas staphylococcus epidermidis (six isolates) was primarily responsible for producing bacteriostatic activity. a comparison of the bacteriostatic substance produced by s. epidermidis isolate 66 with the bactericidal substance produced by s. haemolyt ...	1985	3924654
the effect of estrogen treatment on plasma concentrations of steroid hormones, gonadotropins, prolactin and sex hormone-binding globulin in post-menopausal women.	twenty-one post-menopausal women on no other medication were treated with a low dose (0.625 mg/day) of conjugated equine estrogen (cee) for a mean (+/- sem) period of 2.6 +/- 0.2 mth (range 1.75-4.75). blood samples were collected before and at the completion of therapy, and alterations in the levels of prolactin (prl), follicle-stimulating hormone (fsh), luteinizing hormone (lh), sex hormone-binding globulin (shbg) and certain steroid hormones, including the free testosterone (t) index (t/shbg) ...	1985	3929023
the effect of estrogen treatment on plasma concentrations of steroid hormones, gonadotropins, prolactin and sex hormone-binding globulin in post-menopausal women.	twenty-one post-menopausal women on no other medication were treated with a low dose (0.625 mg/day) of conjugated equine estrogen (cee) for a mean (+/- sem) period of 2.6 +/- 0.2 mth (range 1.75-4.75). blood samples were collected before and at the completion of therapy, and alterations in the levels of prolactin (prl), follicle-stimulating hormone (fsh), luteinizing hormone (lh), sex hormone-binding globulin (shbg) and certain steroid hormones, including the free testosterone (t) index (t/shbg) ...	1985	3929023
prostaglandin-related bone resorption in cultured neonatal mouse calvaria: evaluation of biopotency of nonsteroidal anti-inflammatory drugs.	the objective of this study was the development of an assay based on suppression of endogenous prostaglandin synthesis in cultured neonatal mouse calvaria for evaluation of the biopotency of nonsteroidal anti-inflammatory drugs in bone. in preliminary trials, osteolytic activity due to spontaneous prostaglandin production over a 72 h culture period was found highly variable, and could not be stabilized by addition of the common precursor arachidonic acid to the culture medium. eventually, contin ...	1985	3936122
prostaglandin-related bone resorption in cultured neonatal mouse calvaria: evaluation of biopotency of nonsteroidal anti-inflammatory drugs.	the objective of this study was the development of an assay based on suppression of endogenous prostaglandin synthesis in cultured neonatal mouse calvaria for evaluation of the biopotency of nonsteroidal anti-inflammatory drugs in bone. in preliminary trials, osteolytic activity due to spontaneous prostaglandin production over a 72 h culture period was found highly variable, and could not be stabilized by addition of the common precursor arachidonic acid to the culture medium. eventually, contin ...	1985	3936122
periodontal treatment needs of 35-year-old citizens in oslo.	the periodontal condition was evaluated according to the pi and the periodontal treatment need system (ptns) in a random sample of 35-year-old citizens of oslo. the study demonstrated that periodontal disease was a major dental health problem despite the fact that 88.9% of the subjects visited a dentist regularly. no statistically significant differences were found in pi, ptns and ohi-s values among the different sexes and educational groups. although the majority of the subjects (77.8%) brushed ...	1985	271653
[studies on the uses of the electronic image evaluating device "densitron" for the evaluation of dental radiographs. iii. standardisation in densitometry].	the authors outline in two papers to what extent the interpretability of a radiograph is changed when converted by means of the densitron. they confined themselves to the interpretation of dental films and panoramic radiographs. for reasons of clinical interest, particular attention was paid in dental films on localized processes (such as dental caries), and in panoramic radiographs on possible bone diseases as a sequel or concomitant affection in case of renal insufficiency, diabetes mellitus a ...	1986	268703
identification of coagulase-negative staphylococci isolated from urinary tract infections.	coagulase-negative staphylococci isolated from urinary tract infections were identified using the api staph-ident system. organisms were excluded if there was no sign of pyuria or if normal urethral flora was present in significant amounts. while staphylococcus saprophyticus and staphylococcus epidermidis accounted for 81% of the isolates from females, 87% of isolates from males were s. epidermidis, staphylococcus warneri, or staphylococcus haemolyticus. the females fell into two main age groups ...	1986	3940428
value of the l-pyrrolidonyl-beta-naphthylamide hydrolysis test for identification of select gram-positive cocci.	a 4 hr test for hydrolysis of l-pyrrolidonyl-beta-naphthylamide (pyr) was evaluated for its use in identifying group a beta-hemolytic streptococci, group d enterococci, and staphylococcus haemolyticus. all of the group a streptococci and the enterococci tested were positive in the pyr test, whereas all of the nongroup a, b, or d beta-hemolytic streptococci, all of the viridans, and all of the streptococcus pneumoniae isolates tested were pyr negative. two strains of aerococcus species were pyr p ...	1986	3943290
in vitro studies on the reactivation by oximes of phosphylated acetylcholinesterase--ii. on the formation of o,o-diethyl phosphorylated ache and o-ethyl methylphosphonylated ache and their reactivation by ps2.	the in vitro reactivation profiles of o,o-diethyl phosphorylated ache and o-ethyl methyl phosphonylated ache by p2s (2-hydroxy iminomethyl-1-methyl-pyridinium methane sulphonate) have been determined. whilst reinhibition of the reactivated ache by phosphorylated oxime (pox) is not important in determining the reactivation profile of o,o-diethyl phosphorylated ache, reinhibition of the reactivated ache by phosphonylated oxime can, however, be important in determining the reactivation profile of o ...	1986	3954784
composition and antimicrobic resistance of skin flora in hospitalized and healthy adults.	the aerobic bacterial flora of the skin of the anterior nares, axilla, perineum, and toe web in a group of 37 patients hospitalized for at least 2 weeks was compared with the flora of 30 healthy adults. colony counts were significantly higher for flora of the perineum and toe web in both groups (p less than 0.05). patients had significantly higher carriage rates of proteus, pseudomonas, and candida spp. (p less than 0.01). staphylococcus haemolyticus was isolated more frequently from patients, a ...	1986	3958149
embryo research.		1986	3960103
bidirectional cardiovascular connections between ventrolateral medulla and nucleus of the solitary tract.	single-unit recording experiments were done in chloralose-anesthetized, paralyzed and artificially ventilated cats to identify neurons in ventrolateral medulla (vlm) that send efferent axons directly to the region of the nucleus of the solitary tract (nts) and receive cardiovascular afferent inputs from the carotid sinus (csn) and aortic depressor (adn) nerves and the nts. units in vlm were identified by antidromic excitation to stimulation of functionally and histologically verified sites in th ...	1986	3697701
demonstration of sensory innervation of rat tongue with anterogradely transported horseradish peroxidase.	wheat germ agglutinin-horseradish peroxidase injected into the petrosal ganglion in rats was transported to the posterior tongue in the glossopharyngeal nerve. labelled fibers innervated the ipsilateral foliate papilla only, but both ipsi-and contralateral sides of the single circumvallate papilla. somatosensory fibers in the posterior tongue also were labelled. label crossed transcellularly from the afferent fibers into taste bud cells. use of this method provides more detailed knowledge of sen ...	1986	3697712
homologous cholinergic efferents spared by partial fimbrial lesions contribute to the recovery of hippocampal cholinergic enzymes in adult rats.	cholinergic fibers spared by partial lesions of the fimbrial bundle contribute to the recovery of enzyme markers for hippocampal cholinergic terminals. this recovery, most likely representing structural restoration of cholinergic terminals lost to lesion-induced degeneration, is amplified by a 'conditioning' lesion. this model of functionally relevant postlesion reconstruction, by and of structures within the cns, is suitable to search for means to enhance homotypic collateral sprouting.	1986	3697713
improved methods for purification and depletion of monocytes from bovine peripheral blood mononuclear cells. functional evaluation of monocytes in responses to lectins.	we have compared different techniques for the enrichment and depletion of monocytes from bovine peripheral blood mononuclear cells. adherence to plasma-coated gelatin was the most efficient and reproducible method for enrichment of monocytes (80% monocytes), whereas depletion of peripheral blood mononuclear cells of monocytes (0.3% monocytes and less) was best achieved by defibrination of the blood from which the pbm were separated. in both instances, purity of the cell population could be impro ...	1986	3701072
lamina viii interneurones interposed in crossed reflex pathways in the cat.	the location of a group of interneurones projecting to contralateral motor nuclei has been established using retrograde transneuronal transport of horseradish peroxidase conjugated with wheat germ agglutinin (wga-hrp). after labelling the motoneurones of semitendinosus, medial gastrocnemius or quadriceps muscles, interneurones which were secondarily labelled were found in lamina viii and in the neighbouring narrow strip of lamina vii. they were found to be distributed from the 4th lumbar to the ...	1986	3701648
[lithotripsy by ultrashort pulsation. experimental study in renal lithiasis in the dog].	a lithotriptor delivering ultrashort shockwaves and designed for use under ultrasonographic guidance has recently been developed. immersion in water is unnecessary. the device was experimented in renal lithiasis in dogs. twenty-two animals were treated. in seven, who served as controls, the safety of shots delivered in particular to the lungs, liver, bones and kidneys was demonstrated. furthermore, no cardiac rhythm disorders were recorded. a human stone was implanted in 15 animals. the stone wa ...	1986	3707073
absence of role of endothelium in the response of isolated porcine coronary arteries to acetylcholine.	isolated precontracted arteries of various vascular beds relax in response to acetylcholine only if the endothelium is present. one explanation for this is that this drug stimulates the endothelial cells to release a vasodilator substance that in turn relaxes the underlying smooth muscle. to determine whether this mechanism is concerned also in the acetylcholine contraction of isolated porcine coronary arteries transverse strips of the extramural part of the left circumflex artery were used for ...	1986	3719611
antibodies to bacterial vaccines demonstrating specificity for human choriogonadotropin (hcg) and immunochemical detection of hcg-like factor in subcellular bacterial fractions.	investigations were done to determine whether vaccines prepared with chemically killed staphylococcus haemolyticus ru1 and streptococcus bovis av46 (bacteria that have been demonstrated to express human choriogonadotropin [hcg]-like material on their surface) elicited antibodies in rabbits with specificity for hcg determinants. in addition, the anatomical locus of the hcg-like factor was determined by separation of bacterial subcellular fractions. the results demonstrated that these bacterial va ...	1986	3721581
the sporogonic cycle of plasmodium reichenowi.	plasmodium reichenowi, a malarial parasite of the chimpanzee, was infective to anopheles freeborni, anopheles quadrimaculatus, anopheles stephensi, anopheles maculatus, anopheles dirus, and anopheles culicifacies mosquitoes. anopheles gambiae and anopheles albimanus were not infected. mean oocyst diameters of p. reichenowi were smaller than those of the other chimpanzee parasite, plasmodium schwetzi. sporozoites were present in the salivary glands of an. freeborni at 15 days when held at 25 to 2 ...	1986	3734994
analysis of factors involved in determining urinary bladder voiding cycle in urethan-anesthetized rats.	the mechanism(s) involved in determining the voiding cycle of the rat urinary bladder have been investigated in urethan-anesthetized animals. fluid emission is almost confined to that phase of the voiding cycle which is characterized by the presence of a series of high-frequency oscillations in intraluminal pressure (iphfo). during this phase the mean urethral flow rate reached a maximum and fluid was expelled in a stream-like fashion. the index obtained by multiplying the amplitude of iphfo by ...	1986	3740305
[salmonella agona infections in infants].		1986	3748670
ly164846 in vitro antimicrobial activity testing, including disk diffusion susceptibility tests using 30-microgram disks.	a new oral cephalosporin, ly164846 (eli lilly & co., indianapolis, ind.), was found to have a unique antimicrobial spectrum confined to methicillin-susceptible staphylococcus spp., streptococci (except enterococcus spp.), haemophilus influenzae, branhamella catarrhalis, and some anaerobes. cephalothin in vitro tests (30-microgram disks or dilution) can represent ly164846 for laboratory testing if comparable interpretive breakpoints are applied to strains within the spectrum of ly164846. organism ...	1986	3755018
a rapid assay for activity of phospholipase a2 using radioactive substrate.	a rapid method for the assay of phospholipase a2 has been developed using a radioactive substrate, l-alpha-dipalmitoyl-(2-[9,10(n)-3h]palmitoyl)-phosphatidylcholine. the substrate diluted with cold carrier (1 mm) is dissolved in 80% ethanol containing 25 mm sodium deoxycholate. the enzymatic reaction is performed in 1.0 ml 0.1 m glycine-naoh buffer, ph 9.0, containing 2 mumol cacl2, 10 micrograms bovine serum albumin, 2.5 mumol sodium deoxycholate, 0.01 unit (or less) phospholipase a2, and 40-10 ...	1986	3755302
linkage analysis of loci controlling blood groups and the rectovaginal constriction syndrome in jersey cattle.	the possibility of linkage between the recessive gene controlling the rectovaginal constriction (rvc) syndrome in jersey cattle and 13 loci controlling blood groups and polymorphic proteins was studied. no evidence of close to moderate linkage was found between the rvc locus and any of the systems a, b, c, f, l, s, z, r', hb, tf, am-1 and ca. no definite conclusion was possible with the m system.	1986	3767084
advances in contrast two-dimensional echocardiography.		1986	3768926
the effects of large doses of methylprednisolone on neurologic recovery and survival in the mongolian gerbil following three hours of unilateral carotid occlusion.	using a scoring system designed to assess the severity of neurologic deficit in gerbils during and after temporary unilateral carotid occlusion, the effects of large doses of methylprednisolone sodium succinate (mpss: solu-medrol sterile powder) on experimental stroke have been examined. by scoring gerbils hourly for torso curvature, circling, inability to walk, ptosis, barrel rolling, opisthotonus, generalized seizures, and loss of righting reflex, their level of neurologic deficit can be easil ...	1986	3768981
survival of adrenal gland implants in the neocortex of the rat: a morphological study.	the survival of adrenal gland implantation in the cerebral cortex of the rat was studied. in the present study, mature adrenal gland had survived after six months of implantation. no scar tissue was observed between the adrenal gland cortex and the host cerebral cortex. the implanted tissue showed some reorganization in its cortex and medulla. in the adrenal cortex there was an observable increase in connective tissue fibers and some degeneration of cells. in the medulla, again, both surviving a ...	1986	3780938
intra-nucleus accumbens amphetamine: dose-dependent effects on food intake.	the effects of microinjections into the nucleus accumbens (n.acc.) of 0.0, 2.0 or 8.0 micrograms of (+)-amphetamine sulphate (amph) on food intake and running wheel activity were examined. the 2.0 micrograms dose of amph produced increased food intake while 8.0 micrograms significantly decreased food intake. no effect was found on running wheel activity with the 2.0 micrograms dose, though 8.0 micrograms significantly increased the number of wheel revolutions with respect to the saline group. re ...	1986	3809216
[a limiting factor of glycolysis in bone and callus].	phosphofructokinase reaction was shown to be a key step of glycolysis after addition of various substrates to postmitochondrial fraction of bone and callus in vitro. administration of fructose-1,6-diphosphate into the animals with femur fracture within a first week of the fracture consolidation led to activation of glycolysis in regenerate as a result of which more mature callus was formed within 10 weeks.	1986	3811293
expression of agrobacterium tumefaciens t-dna gene 7 in xenopus laevis oocytes.	the expression of agrobacterium tumefaciens t-dna gene 7 was investigated in xenopus laevis oocytes. cloned dna injected into oocytes consisted of t-dna sequences derived from octopine type ti plasmid b6-806 and t-dna attached to plant dna sequences at the left junction in crown gall tumors. transcription initiation sites observed in oocytes were similar to those for transcript 7 in crown gall tumors. quantitative differences in transcription occurred depending on the flanking sequences of the i ...	1986	3814121
enhancement of hemopoietic recovery by indomethacin after sublethal whole-body gamma irradiation.	the effect of the non-steroid anti-inflammatory drug, indomethacin, a potent prostaglandin synthesis inhibitor, on the recovery of hemopoiesis was investigated in sublethally gamma irradiated mice. treatment with indomethacin after irradiation was found to increase the granulocyte and lymphocyte counts in peripheral blood. furthermore, an increased rate of the restitution of bone marrow cellularity and of the spleen weight was observed. using the method of 125iodo-deoxyuridine uptake in the sple ...	1986	3020886
effect of forphenicinol on the production of ia-positive macrophages in mice with or without l1210 leukemia and on the growth of l1210 in immunized mice.	oral administration of forphenicinol, s-2-(3-hydroxy-4-hydroxymethylphenyl)glycine, increased the production of ia-positive peritoneal macrophages in healthy mice. moreover, forphenicinol increased ia-positive macrophages in l1210-bearing mice. though forphenicinol was ineffective against mouse leukemia l1210 when administered alone, in combination with l1210 vaccine it prolonged the survival time of l1210-bearing mice and the increase in the number of ia-positive macrophages in peritoneal cavit ...	1986	3458691
etiopathogenesis of uric acid and ammonium urate uroliths in non-dalmatian dogs.	the etiopathogenesis of uric acid, sodium acid urate, and ammonium acid urate uroliths in non-dalmatian dogs appears to be a complex phenomenon. it may involve one or more pathologic and/or physiologic processes acting independently or in concert to increase urinary concentration of lithogenic substances that result in initiation, growth, and retention of urate uroliths. increased urine uric acid concentration and/or urinary excretion of uric acid appear to be primary predisposing factors in ura ...	1986	3518203
status of basic external human fertilization.		1986	3529121
pervasive developmental disorders and immunological tolerance.	a wide range of studies in man and other species suggest that early compromise of immunological tolerance (both maternal-fetal and self) may lead to severe and varied cognitive deficits. this article briefly reviews what is known of the genesis and maintenance of normal tolerance and current ideas on pathological deviances in tolerance. these ideas are discussed in relation to risk factor, family, twin, biochemical, anatomical, and immunological studies of pervasive developmental disorders (part ...	1986	3532100
in vitro susceptibilities of four species of coagulase-negative staphylococci.	the in vitro susceptibilities of 260 strains of coagulase-negative staphylococci to penicillin g, oxacillin, nafcillin, methicillin, cephalothin, and seven non-beta-lactam antimicrobial agents were determined and compared with the susceptibilities of 54 strains of staphylococcus aureus with known patterns of susceptibility. penicillin g susceptibility for s. aureus, staphylococcus epidermidis, staphylococcus haemolyticus, and staphylococcus hominis was readily determined by using beta-lactamase ...	1986	3539009
acetyl-homocysteine-thiolactone-induced increase of superoxide dismutase counteracts the effect of subdiabetogenic doses of streptozocin.	acetyl-homocysteine-thiolactone (cyt) is an organic thio compound that exerts free radical scavenger activity and increases superoxide dismutase (sod) activity. administration of 32 mg cyt/kg body wt/day/30 days in rats increased sod activity in erythrocytes by 126%, and in pancreatic islets by 202%. treatment affected only the cu-zn fraction of the enzyme. transmission electron microscope observations showed that the damage to the pancreatic beta cells induced by single or multiple subdiabetoge ...	1986	2937674
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.	in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...	1986	2942039
differential effects of recombinant human leukocyte interferons on cell surface antigen expression.	human leukocyte (alpha) interferon (ifn-alpha) is composed of a multigene family within which at least eight different species have been expressed in escherichia coli, isolated, and shown to exert a wide range of biological activities on different human target cells. in this study we utilized eight species of ifn-alpha (a, b, c, d, f, i, j, and k) and investigated their respective capabilities to alter the proliferation of a human breast carcinoma cell line (mcf-7). the antigens studied were all ...	1986	2944575
mechanisms of lymphocyte adhesion to human vascular endothelial cells in culture. t lymphocyte adhesion to endothelial cells through endothelial hla-dr antigens induced by gamma interferon.	the effects of interferons (ifns) on lymphocyte adhesion to cultured human vascular endothelial cells (ec) were investigated using an in vitro assay. endothelial cells obtained from umbilical vein were first cultured at a low density with a conditioned medium (cm) from 12-o-tetra decanoylphorbol 13-acetate-concanavalin a (tpa-con a) stimulated human peripheral blood lymphocytes (pbl), or with recombinant (r) gamma interferon (ifn-gamma) or r alpha interferon (ifn-alpha), and then were incubated ...	1986	3084562
air-1, a newly found locus on mouse chromosome 16 encoding a trans-acting activator factor for mhc class ii gene expression.	rj 2.2.5 is a human b cell line that has lost the capacity to express mhc class ii genes. the human class ii-positive phenotype is restored in somatic cell hybrids between rj 2.2.5 and mouse spleen cells. by karyotype and molecular studies of an informative family of hybrids we have now shown that the reexpression of human class ii gene products, as well as the maintenance of the mouse class ii-positive phenotype, correlates with the presence of mouse chromosome 16. thus, the existence on this m ...	1986	3088202
molecular characterization of ro(ssa) and la(ssb) proteins.	ro(ssa) and la(ssb) proteins were characterized using human spleen extract as antigen and monospecific anti-ro(ssa) and anti-la(ssb) sera. the antigens were partially purified by deae cellulose and sephacryl chromatography, and then were electrotransferred to nitrocellulose sheets after sds-page electrophoresis. the anti-ro(ssa) sera had reacted with a polypeptidic band of 58 kilodaltons (kda), meanwhile the la(ssb) antisera reacted with 2 polypeptidic bands of 42 and 40 kda. the molecular weigh ...	1986	3088274
effect(s) of lipopolysaccharide on lectin-induced t-cell activation.	the present study focuses on the effect of lipopolysaccharide (lps) on the cellular events leading to t-cell activation by concanavalin a (con a). interleukin 2 (il-2) production is much reduced in con a-stimulated cultures of spleen cells derived from lps-treated mice. this depressed il-2 synthesis is not related to the eventual activity of lps-activated suppressive b cells. rather, it reflects an ineffective collaboration between adherent cells and t lymphocytes. the low level of il-2 produced ...	1986	3091272
nucleotide sequence of gene lina encoding resistance to lincosamides in staphylococcus haemolyticus.	we have determined the nucleotide sequence of gene lina in plasmid pip855, which confers resistance to lincomycin by inactivating it in staphylococcus haemolyticus. the gene was defined by start and stop codons and an open reading frame of 483 bp corresponding to a product with an mr of 19020. the apparent size of the resistance-conferring protein was 21 kda, when a minicell extract was electrophoresed in the sodium dodecyl sulfate-polyacrylamide gels. full expression of lina was obtained both i ...	1986	3091456
biomonitoring of industrial dusts on animals. ii. bioindication on alveolar macrophages.	rats and rabbits were exposed through the respiratory system to industrial dusts (magnesite emissions, solid wastes from nickel refinery dump, cement emissions) at biomonitory stations or in experimental chamber. following exposure the animals were killed, the alveolar macrophages isolated and acid phosphatase and beta-glucuronidase estimated in the isolated cells. the activity of both enzymes was enhanced in the exposed animals in all cases. the enhancement was dependent on the length of exposu ...	1986	3647712
a protocol and guide for the in vivo rat hepatocyte dna-repair assay.	the in vivo rat hepatocyte dna-repair assay is a valuable tool in assessing the genotoxic activity of chemical agents. an advantage of the system is that it reflects the complex patterns of uptake, distribution, metabolism, detoxification and excretion that actually occur in the whole animal. this article provides a typical procedure and guidelines for conducting the rat in vivo hepatocyte dna-repair assay.	1987	3657828
effect of egg as a sole diet on hepatic lipid peroxidation and related defence system in the rat.		1987	3666776
short-term effects of two fusarium toxins, diacetoxyscirpenol and neosolaniol monoacetate, in male wistar rats.	this study compared the pathological and haematological effects of neosolaniol monoacetate in male wistar rats with those of diacetoxyscirpenol, a highly toxic trichothecene mycotoxin. both toxins caused statistically significant decreases in erythrocyte counts and increased the proportion of larger platelets compared to controls when dosed at 1 mg/kg body weight three times/wk for up to 5 wk. lesions caused by treatment with either toxin at this dosage level were mild and were seen--mainly afte ...	1987	3679023
practolol inhibits human skin fibroblast cell mat hydroxyproline accumulation.	despite being poorly absorbed practolol (n-4-2-hydroxy-3-(1-methyl-ethyl)-amino propoxy phenyl acetamine) inhibited the accumulation of cell mat hydroxyproline, a measure of collagen synthesis, by human skin fibroblasts (dt2ph) in vitro, (id50 0.8 x 10(-3) m). the degree of inhibition was dependent on the concentration of practolol used and the incubation time. neither preinitiation of collagen synthesis nor omitting ascorbic acid from the incubation medium modified this inhibitory action. in co ...	1987	3687592
na+-independent, pyridine nucleotide-linked efflux of ca2+ from preloaded rat heart mitochondria: induction by chlortetracycline.		1987	3689435
na+-independent, pyridine nucleotide-linked efflux of ca2+ from preloaded rat heart mitochondria: induction by chlortetracycline.		1987	3689435
[iron, manganese and cobalt levels in soil, pasture grass and bodies of young bulls after irrigation of the soil with waste water].	the effect of irrigation of meadows with the water of the river. ner on the levels of iron, manganese and cobalt in the soil and vegetation was studied. experiments were also carried out on 30 young bulls fed with the hay grown on these meadows. the levels of iron and cobalt were determined in the blood plasma, and the manganese level in the hair of the bulls. the experiments were carried out twice during 93 days. the animals were divided into 3 groups. group i and ii received the hay from the i ...	1987	3697343
human choriogonadotropin-like material in bacteria of different species: electron microscopy and immunocytochemical studies with monoclonal and polyclonal antibodies.	immunocytochemical studies using antisera to whole human choriogonadotropin (hcg), to its alpha- and beta-subunits and to the cooh-terminal peptide of hcg beta, and two monoclonal antibodies to hcg beta, demonstrated expression of hcg-like material, its individual subunits and/or fragments in nine bacterial strains. seven of these were isolated from patients with cancer and were definitely identified as streptococcus faecalis (three strains), staphylococcus haemolyticus (two strains) and staphyl ...	1987	3116165
[primary leiomyosarcoma of the pulmonary artery. apropos of a case].	a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ...	1987	3122693
in vitro analysis of t-cell-mediated immunity to intact eggs in murine experimental schistosoma japonicum infection.	to characterize the mechanisms of induction and regulation of the cell population involved in granuloma formation around eggs of schistosoma japonicum, we utilized a simple method of in vitro experiments. lyt1+2-t cells were essential for in vitro responses to the intact s. japonicum eggs, which were assumed to be comparable to in vivo granulomatous responses. t-cell responses seemed to be macrophage-dependent, and responding t cells produced il-2-like activities in the culture supernatant. sera ...	1987	3127618
quality control limits for teicoplanin susceptibility tests and confirmation of disk diffusion interpretive criteria.	for monitoring the performance of teicoplanin susceptibility tests, the following quality control limits are recommended: staphylococcus aureus atcc 29213, mic of 0.12 to 0.5 micrograms/ml; enterococcus faecalis atcc 29212, mic of 0.06 to 0.25 micrograms/ml; and s. aureus atcc 25923, zones 15 to 19 mm in diameter (30-micrograms disks). however, some lots of mueller-hinton agar provided unusually large zones of inhibition with both vancomycin and teicoplanin disks, and these lots should be exclud ...	1987	2958498
inhibition of adrenomedullary catecholamine release by propranolol isomers and clonidine involving mechanisms unrelated to adrenoceptors.	1 transmural electrical stimulation (10 hz, 40 v, 1 ms for 60s) increased total catecholamine secretion from perfused cat adrenal glands; this response was enhanced by neostigmine and inhibited by mecamylamine, suggesting that release of acetylcholine from splanchnic nerve terminals was stimulating nicotinic receptors and enhancing catecholamine secretion. 2 isoprenaline, (-)-propranolol and (+)-propranolol (10(-7)-10(-5)m) inhibited the electrically-evoked secretory response by 40-70%; similar ...	1987	2827826
keratinolytic activity of cutaneous and oral bacteria.	a test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (ph 7.2) with 1 mm dithiothreitol. the degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. several oral and cutaneous bacteria were investigated: bacteroides gingivalis, bacteroides intermedius, treponema dent ...	1987	2434427
comparison of the acute phase response of cultured morris hepatoma 7777 cells and of rat hepatocytes.	isolated morris hepatoma cells (line 7777) or adult rat hepatocytes were cultured for 3 days and daily production of four plasma proteins was estimated in the cell media by rocket immunoelectrophoresis with monospecific antisera. addition of cytokines from rat peritoneal macrophages to cultured hepatocytes or hepatoma cells augmented accumulation in the medium of two positive acute phase proteins: fibrinogen (fib) and cysteine proteinase inhibitor (cpi). at the same time synthesis of alpha-fetop ...	1987	2443154
genotoxicity studies with two antichagasic drugs.		1987	3553925
suppression of autoimmunity to rat male accessory glands: possible relevance to tumor-host responsiveness.		1987	3592057
polyunsaturated fatty acids augment free radical generation in tumor cells in vitro.	polyunsaturated fatty acids (pufas) have been shown to inhibit both normal and tumor cell growth in vitro. as pufas are known to induce a respiratory burst and free radical generation in polymorphonuclear leukocytes and since free radicals are toxic to cells, we investigated the effect of pufas on a measure of free radical generation (nitroblue tetrazolium reduction) in normal human fibroblasts and breast cancer cells in vitro. results suggested that linoleate (la), gamma-linolenate (gla), arach ...	1987	3593336
[lipid metabolism in daunomycin induced nephrotic rats (part iii)].		1987	3599547
albendazole therapy in alveolar hydatid disease: a report of favorable results in two patients after short-term therapy.	albendazole was administered preoperatively to two patients with active alveolar hydatid disease for 58 and 84 days. vesicles of larval echinococcus multilocularis obtained from surgical tissues were inoculated into red-backed voles for in vivo testing viability. no proliferation of the larval cestode had occurred when the animals were dissected three months post-inoculation. these findings suggest that short-term therapy with albendazole was effective in killing the larval cestode in these two ...	1987	3605498
[studies on the metabolic fate of 14c-rokitamycin. i. absorption, distribution, metabolism and excretion in rats].	blood concentrations of 14c-rokitamycin (14c-tms-19-q) reached their peaks at 1 hour after a single oral (200 mg/kg) administration to male and female rats, and they were 28.0 +/- 0.8 and 24.9 +/- 2.0 micrograms/ml, respectively. no significant differences were observed between male and female in auc values or maximum blood concentrations. the distribution of tms-19-q was good, and concentrations of 14c were high in liver, kidney, spleen, pancreas, adrenal, pituitary gland, thyroid, trachea, exo ...	1987	3613074