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serological studies of peptostreptococci using an indirect fluorescent antibody test.members of the genus peptostreptococcus are frequent isolates from periodontal lesions. a study was undertaken for determination of the serological relationships of oral and non-oral strains of several species of this genus. eighty-nine strains of peptostreptococci representing seven species were tested by means of an indirect fluorescent antibody technique (ifa), with rabbit antisera to peptostreptococcus anaerobius atcc 27337, peptostreptococcus micros vpi 2618-a, and peptostreptococcus produc ...19892685068
anaerobic bacteria in brain abscess, a review; observation of 18 consecutive cases.the aerobic and anaerobic bacterial flora of 18 consecutive cases of brain abscesses were studied. two cases were sterile. in five cases there was a single bacterial isolate. in five cases there was a single bacterial isolate the organism being staphylococcus aureus in two cases, bacteroides fragilis in two cases and peptostreptococcus anaerobius in one case. in the remaining eleven cases, more than one organism was isolated. a combination of anaerobe with an aerobe was observed in five cases an ...19892687167
adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine.a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ...19892770471
[antibody-dependent harmful effect of non-immune spleen cells in acute experimental tick-borne encephalitis in mice].the injection of nonprotective dilutions of immune serum and nonimmune spleen cells into mice infected with tick-borne encephalitis virus induced a sharply pronounced immunopathological effect: the mean survival time of the recipients decreased by 3.6 days in comparison with the control animals. this effect was not linked with the increased replication of the virus in the brain. the antibody-dependent damaging action of spleen cells could be reproduced by using the cells of both syngeneic and al ...19892786665
disease response to tobacco mosaic virus in transgenic tobacco plants that constitutively express the pathogenesis-related pr1b gene.correlation of the temporal and spacial pattern of induction of the pathogenesis-related (pr) genes pr1a, pr1b, and pr1c with viral infections in certain tobacco cultivars has implicated pr proteins in viral disease resistance. to test whether the pr1 proteins of tobacco are involved in viral resistance, transgenic nicotiana tabacum plants were constructed which constitutively express the pr1b gene. this protein was secreted from cells of transgenic plants and accumulated in the extracellular sp ...19892815592
polysaccharides from peptostreptococcus anaerobius and structure of the species-specific antigen.the cell-envelope antigens of peptostreptococcus anaerobius were extracted from intact cells by autoclave or alkaline treatment. the purified species-specific antigen (g) was identified among several polysaccharides obtained from the extracts by successive treatments with ribonuclease and pronase followed by ion-exchange and gel-filtration chromatography. g was investigated by 13c- and 31p-n.m.r. spectroscopy, titrimetry, elemental analysis, and gas-liquid chromatography. oxidation of g with nai ...19902076510
bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment.phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ...19902098335
a rapid diagnosis of anaerobic infection in the oro-maxillary region by gas-liquid chromatography.the relationship between volatile fatty acids (vfas) in pus and infecting bacterial species was examined in order to establish a rapid identification system for anaerobic microorganisms in purulent inflammation in the oro-maxillary region. vfas were detected by the direct injection of pus into gas-liquid chromatography (glc). bacterial examination was carried out by anaerobic culture using blood agar plates. the bacterial identification was carried out mainly according to the vpi manual. analysi ...19902131168
demonstration of the heterogeneity of the kappa-opioid receptors in guinea-pig cerebellum using selective and nonselective drugs.in guinea-pig cerebellum, saturation studies reveal that the nonselective opioid [3h]ethylketazocine has a binding capacity (r) of 6.79 pmol/g tissue which is similar to the sum of the individual r values of the mu-, delta- and kappa 1-selective opioids. conversely, the binding parameters of the nonselective opioid [3h]bremazocine are best-fitted to a two-site model (kd1 = 0.12 nm, r1 = 11.3 pmol/g tissue; kd2 = 6.03 nm, r2 = 9.09 pmol/g tissue) with an r total value of 20.3 pmol/g tissue which ...19902164939
[new antitubercular drugs]. 19902168041
transferable tet m in fusobacterium nucleatum.two tetracycline-resistant fusobacterium nucleatum strains were able to transfer, by conjugation, tetracycline resistance and the tet m determinant to recipient f. nucleatum, peptostreptococcus anaerobius, and enterococcus faecalis strains at a frequency of 10(-1) to 10(-8).19902178339
formation of amphetamine from its nitro analogue by anaerobic intestinal bacteria.1. microbial reduction of 1-phenyl-2-nitropropane 1 was carried out using 40 strains of intestinal anaerobic bacteria. among them, 12 strains (mitsuokella multiacidus, clostridium perfringens, c. innocuum, c. clostiriiforme, c. difficile, c. butyricum, c. sp., eubacterium limosum, e. aerofaciens, e. multiforme, peptostreptococcus anaerobius and p. productus) had the ability to reduce 1 to amphetamine 2 (0.1-1% yield). 2. clostridium species were more active than another intestinal anaerobic bact ...19902219956
use of modified norleucine-tyrosine broth in identification of peptostreptococcus anaerobius.gas-liquid chromatography was employed to analyze the volatile and nonvolatile acids produced in modified norleucine-tyrosine (mnt) broth by various gram-positive cocci. the mnt broth consists of 0.5% trypticase (bbl microbiology systems, cockeysville, md.), 0.5% yeast extract (difco laboratories, detroit, mich.), 0.2% l-norleucine, and 0.1% l-tyrosine. the microorganisms included reference strains and clinical isolates of peptostreptococcus spp. (p. anaerobius, p. asaccharolyticus, p. indolicus ...19902229394
[a clinical study on ceftazidime in the treatment of intractable respiratory infections].ceftazidime (caz) was administered to 51 patients (37 males, 14 females) with respiratory infections including severe cases, accompanied by various underlying respiratory diseases. the clinical efficacy and side effects of caz were investigated. the mean age and body weight of these 51 cases were 62.6 years and 48.0 kg, respectively. caz was administered by intravenous drip infusion (daily dose of 2-4 g) for a mean of 14.7 days to a mean total dose of 56.7 g. clinical efficacy rates were 64% (18 ...19902232147
isolation and identification of anaerobic bacteria from ovine foot rot in spain.a microbiological study was made of 125 merino sheep showing clinical signs of foot rot. a total of 435 strictly anaerobic strains were isolated, belonging to the following genera: bacteroides, peptostreptococcus, tissierella, fusobacterium, megasphaera, eubacterium, acidaminococcus, clostridium, peptococcus and propionibacterium. of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: bacteroides nodosus, b putredinis, b buccae, b ruminicola subspecies ...19902236925
obligately anaerobic bacterial species isolated from foot-rot lesions in goats.lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ...19902271912
the obligate and facultatively anaerobic bacterial flora of the normal feline gingival margin.samples from the gingival margins of 14 cats considered normal on clinical examination were cultured for facultative and obligate anaerobic bacteria. all mouths were free from any gingival marginal inflammation and tartar build-up; all cats were between 6 and 12 months of age. a mixed growth was obtained from all samples. the mean number of bacterial species per sample was 10.7 with a range of 7-16 isolates. of the 150 isolates processed, 109 (72.66%) were obligate anaerobes. of the facultativel ...19902353448
comparative efficacy of cefoperazone, cefoperazone plus sulbactam, ciprofloxacin, clindamycin, metronidazole, and penicillin g against anaerobic bacteria in an animal model.treatment efficacy of various antimicrobial regimens against anaerobes was studied in semipermeable chambers simulating a closed-space, locally neutropenic infection site in rabbits. bacteroides fragilis, bacteroides melaninogenicus, clostridium perfringens, and peptostreptococcus anaerobius were inoculated (at a mean of 5.3 log10 cfu/ml in prereduced pooled rabbit serum) into the chambers (one isolate per chamber) in triplicate. antimicrobial therapy consisted of cefoperazone, cefoperazone plus ...19901967626
[anaerobic bacteria and their role in recurrent tonsillitis].the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ...19901981606
abdominal ultrasonography in pediatrics. 1990171943
the differentiation of asaccharolytic anaerobic gram-positive cocci by protein electrophoresis.conventional biochemical and antibiotic sensitivity tests were used to allocate 87 clinical isolates of anaerobic gram-positive cocci to currently recognised species, in comparison with type and other authentic reference strains. whole-cell protein electrophoresis was then performed with extracts of each strain. allowing for difficulties of standardisation, it was possible to allocate most of the organisms to species-related groups on the basis of protein patterns. organisms identified conventio ...19912056518
thermogenic and hormonal responses to palatable protein and carbohydrate rich food.simultaneous variations of oxygen consumption, and plasma insulin and norepinephrine were measured during the postprandial cephalic and gastrointestinal phases of feeding in six human subjects following the ingestion of various nutrients. on alternative days the subjects were given foods (1280 kjoules) either rich in carbohydrates (sugar pie) or in proteins (fish). both nutrients produced an initial (0-40 min) enhanced thermogenesis and an early (2 min) cephalic insulin release. during that peri ...19911774019
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta).the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ...19911803013
[empyema due to an anaerobic streptococcus in childhood].anaerobic infections are quite rare in pediatric age, being that, they affect only neonates and immunodepressed patients. we think to be somewhat interesting to describe the case of our patient, a 9 year old boy, unaffected by any predisposing factor, came under our observation because of a severe respiratory distress. he showed evident clinical and radiological signs of pleural effusion in the right lung, together with a gas coil in the upper field and a left mediastinal shifting. a thoracentes ...19911806927
formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria.during the course of our studies on the metabolism of iridoid glycosides by human intestinal bacteria, we found that geniposide (1) and gardenoside (4) were transformed to new nitrogen-containing compounds, genipinine (3) and gardenine (6), respectively, along with the known aglycones. although the amounts of new metabolites were somewhat lower than those of the aglycones, they were quantitatively analyzed by means of liquid chromatography/mass spectrometry (lc/ms). of 25 strains of human intest ...19911818345
bidirectional morphological changes induced by dexamethasone in morris hepatoma cell lines mca-rh7777 and mca-rh8994: independence of fibronectin and its receptor.two morris hepatoma-derived cell lines, mca-rh7777 (7777) and mca-rh8994 (8994), exhibit different alterations in morphology upon exposure to glucocorticoid. after treatment with synthetic glucocorticoid dexamethasone (dex), 7777 cells show increased adhesiveness and more flattened shape, while dex-treated 8994 cells show decreased adhesiveness to substratum and exhibit a marked increase of round and detached cells. since fibronectin has been thought to play an important role in cell adhesivenes ...19911829041
human and health rights of palestinians in the occupied territories. essay review. 19911865855
association of antisense oligonucleotides with lipoproteins prolongs the plasma half-life and modifies the tissue distribution.in order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. a 16-mer oligonucleotide (odn) was derivatized at the 5' prime through a 32p phosphate spacer with cholesterol, yielding a 32p-labeled amphiphatic cholesteryl-oligonucleotide (cholodn). incubation of cholodn with low-density lipoprotein (ldl) for 2 hr at 37 degrees c resulted in the formation of a cholodn-ldl complex that migrate ...19911891360
protein-kinase-c-independent activation of arachidonate release and prostaglandin e2 formation in macrophages interacting with certain bacteria.certain bacterial species, of which we selected fusobacterium nucleatum, gardnerella vaginalis, peptostreptococcus anaerobius and propionibacterium acnes, were found to induce release of arachidonic acid in a dose- and time-dependent manner in mouse macrophages. the release of arachidonic acid showed a characteristic lag period of approximately 10 min and was accompanied by selective transformation into prostaglandin e2. bacteria killed by various methods caused a similar response, indicating th ...19911915341
tetracycline resistance in peptostreptococcus species.of 15 peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the tet k, tet m, or tet o determinant. a donor peptostreptococcus anaerobius strain carrying the three determinants could transfer tet m to p. anaerobius and fusobacterium nucleatum recipients but not to an enterococcus faecalis recipient, while neither tet k nor tet o was transferred.19911929341
new strategies in the development of thrombolytic agents. 19911954777
[treatment and monitoring of primary hypothyroidism].treatment of primary hypothyroidism requires a long-term hormonal therapy with levothyroxine which restores a normal tsh value. in a series of 192 patients followed up for at least 3 years we tried to evaluate the variability of maintenance dosage and to analyse the factors predictive of replacement dosage. the l-t4 dosage varied from one individual to the other, from 0.6 to 3.2 micrograms/kg. the mean maintenance dosage (118 +/- 38 micrograms/day) was similar in men and women weight for weight, ...19921287777
antibacterial efficacy of root canal medicaments.the antibacterial efficacy of four endodontic medicaments was studied in vitro. calcium hydroxide (calacept), camphorated parachlorophenol (cpcp), cresophene, and 2% iodine-potassium iodide (iki) were tested against four anaerobic microorganisms, streptococcus mutans, peptostreptococcus anaerobius, porphyromonus gingivalis and fusobacterium nucleatum, for 10 or 15 min. iki was effective only against f. nucleatum and p. gingivalis, and the others were effective against all four microorganisms.19921337555
detubularization-induced contractile response change of the ileum following ileocystoplasty.we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ...19921351953
periodontal bone loss in mice induced by different periodontopathic organisms.periodontal bone loss in mice orally inoculated with peptostreptococcus anaerobius, pept. magnus and actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice. six-to-8-week-old balb/c mice were inoculated with 1 x 10(5), 1 x 10(7) or 1 x 10(9) colony-forming units (c.f.u.) of bacteria in 50 microliters of medium. ten mice received each concentration of bacteria and 10 sham-inoculated mice acted as controls. five mice from each of the groups were killed 6 weeks after inoc ...19921353347
experimentally induced infection by oral anaerobic microorganisms in a mouse model.a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ...19921408362
experimental studies of survival of anaerobic bacteria at 4 degrees c and 22 degrees c in two different transport systems.the survival of anaerobic bacteria on swabs in two different transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart transport medium (mst), and a viscose swab in modified siff transport medium (btm) (bionor). the following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; fusobacterium necrophorum, bacteroides melaninogenicus, bacteroides inte ...19921472363
the relative lethality of intestinal bacteria for gnotobiotic rats with experimental intestinal strangulation.in eight experiments utilizing 28 animals each, germfree rats with and without ischemically or hemorrhagically strangulated closed loop intestinal segments were contaminated with either one of four common intestinal anaerobic bacteria, or a combination of two or three bacteria previously found to be innocuous in pure culture. the results showed that: a) in pure culture, bacteroides fragilis, bacteroides melaninogenicus, and peptostreptococcus anaerobius were innocuous; b) fusobacterium necrophor ...19921479304
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay.xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ...19921563644
[the role anaerobes in dermatitis digitalis et interdigitalis in cattle].altogether 52 tissue samples and swabs from the corium of the claw of diseased cattle were investigated. microbiological examinations revealed the presence of 11 strictly anaerobic bacterial strains that belonged to the species peptococcus asacharolyticus, pc. sacharolyticus, peptostreptococcus anaerobius, bacteroides asacharolyticus and b. melaninogenicus. the remaining isolates were classified as bacteroides, fusobacterium, streptococcus and clostridium. in the above-mentioned strains observat ...19938259638
the normal vaginal flora, h2o2-producing lactobacilli, and bacterial vaginosis in pregnant women.in this study of the vaginal flora of 171 pregnant women in labor at term, the flora was categorized as normal (lactobacillus predominant), intermediate, or representative of bacterial vaginosis (bv) on the basis of a vaginal smear. bv was diagnosed in 39 women (23%); the vaginal flora was classified as normal in 50% of cases and as intermediate in 27%. h2o2-producing lactobacilli were recovered from 5% of women with bv, 37% of those with an intermediate flora, and 61% of those with a normal flo ...19938324131
peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report.peritonsillar abscess is a potentially life-threatening complication of acute tonsillitis. on occasion, peritonsillar abscess can extend to neck spaces and/or to the mediastinum. we describe a case of a patient with a peritonsillar abscess that extended to the neck, producing bilateral retropharyngeal abscesses and myonecrosis of the strap muscles. culture of a specimen of the necrotic muscle yielded prevotella intermedia, prevotella buccae, lactobacillus catenaforme, another lactobacillus speci ...19938324135
[actinomycosis infection of the cerebellum].the case is presented of a 37-year-old male with a cerebellar abscess due to actinomyces israelii. the patient was admitted after dental surgery because of fever and symptoms indicative of raised, intracranial pressure. no abnormalities were detected in peripheral blood and cerebrospinal fluid. ct scan revealed a space-occupying lesion in the left cerebellar hemisphere with perifocal oedema and occlusive hydrocephalus. after emergency external ventriculostomy complete removal of the mass was ach ...19938333207
the influence of race and gender on periodontal microflora.previous studies have demonstrated that demographic characteristics of subject populations influence both the incidence of periodontal diseases and various aspects of host responses to periodontal bacteria. in this study we analyzed the components of the subgingival microflora from individuals with adult periodontitis, early onset periodontitis, gingivitis, and periodontal health as a function of gender and race (black and white). clinical categories were analyzed individually so that there were ...19938387107
phylogeny of the ammonia-producing ruminal bacteria peptostreptococcus anaerobius, clostridium sticklandii, and clostridium aminophilum sp. nov.in previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. three isolates, strains c, ft (t = type strain), and sr, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. on the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. in this study, the 16s rrna sequences of these isolat ...19938427801
intrapleural streptokinase in experimental empyema.intrapleural streptokinase has been used in multiloculated empyemas to enhance pleural space drainage, presumably by causing fibrinolysis of the interlocular septae. we evaluated the efficacy and safety of daily administration of 10,000 u intrapleural streptokinase or equal volumes of saline to enhance resolution of experimental empyema in the rabbit pleural space. seventy-two hours after intrapleural turpentine, 10(8) colony-forming units each of escherichia coli, peptostreptococcus anaerobius, ...19938466134
in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria.the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ...19938517721
an in-vitro study of the comparative viability on different swab types of simulated specimens of bacteria commonly present in oro-facial infections.an in-vitro study was undertaken to investigate the survival on bacteriological swabs of three potentially pathogenic organisms found in oro-facial infections, by using simulated clinical specimens incubated in the presence or absence of amies transport medium. standard inocula of pure cultures of streptococcus milleri, prevotella melaninogenica and peptostreptococcus anaerobius were soaked on to swabs and plated out on to solid media at various intervals up to 3 days, enabling an estimate of th ...19938099810
cytotoxicity versus antibacterial activity of some antiseptics in vitro.through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. a follow-up study was conducted to examine the germicidal effect of 0.05% naocl, 10 mg/ml metronidazole and supernatant of ca (oh)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. in vitro testi ...19938100851
electrogenic glutamine uptake by peptostreptococcus anaerobius and generation of a transmembrane potential.peptostreptococcus anaerobius converted glutamine stoichiometrically to ammonia and pyroglutamic acid, and the eadie-hofstee plot of glutamine transport was biphasic. high-affinity, sodium-dependent glutamine transport (affinity constant [kt] of 1.5 microm) could be driven by the chemical gradient of sodium, and more than 20 mm sodium was required for half-maximal velocity. high-affinity glutamine transport was not stimulated or inhibited by a membrane potential (delta psi). low-affinity glutami ...19948113169
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...19948123556
in vitro antibacterial activity of fk037, a new parenteral broad-spectrum cephalosporin, against recent clinical isolates in the fields of obstetrics and gynecology.the antibacterial activity of a new parenteral cephalosporin, fk037 was assessed against recent aerobic and anaerobic strains isolated from patients in the fields of obstetrics and gynecology during the period between january 1992 and june 1993. the mics of fk037 for 90% of the clinical isolates tested were 0.10 microgram/ml for escherichia coli and klebsiella pneumoniae, 0.20 microgram/ml for streptococcus agalactiae, 0.39 microgram/ml for gardnerella vaginalis, 0.78 microgram/ml for staphyloco ...19948205934
the gingival immune response to periodontal pathogens in juvenile periodontitis.a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ...19947870467
rapid diagnosis of anaerobic gram-positive cocci by salt tolerance.the maximum salt tolerance was 2.5% in cases of peptostreptococcus anaerobius and peptococcus prevotii, 3% in ruminococcus albus, 4% in acidaminococcus fermentans, peptococcus niger and megasphaera elsdenii and 5% in peptococcus magnus. the biochemical tests being largely inactive and time consuming the findings of salt tolerance if combined with those of morphological data provide clue to the rapid identification of gram-positive anaerobic cocci.19947963612
identification of five peptostreptococcus species isolated predominantly from the female genital tract by using the rapid id32a system.the rapid id32a kit (biomérieux vitek, inc., hazelwood, mo.) was evaluated for its ability to identify peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (virginia polytechnic institute), the current "gold standard" method. a total of 5 peptostreptococcus american type culture collection strains and 95 clinical isolates comprising peptostreptococcus anaerobius, p. asaccharolyticus, p. magnus, p. micros, and p. prevotii isolates were included for ...19948051260
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
the in vitro activity of trospectomycin against anaerobic bacteria.the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...19958667035
budgerigar breeders' hypersensitivity pneumonitis presenting as chronic bronchitis with purulent sputum.a 49-year-old woman complained of cough and a purulent sputum (about 40 ml/day). she kept budgerigars for a period of 30 years, she first noticed a productive cough 10 years previously. her chest roentgenogram revealed a diffuse reticulonodular pattern. she was diagnosed as having a chronic type of budgerigar breeder's lung associated with chronic bronchitis. peptostreptococcus anaerobius was cultured from her sputum, bronchoalveolar lavage fluid, as well as from droppings of her budgerigar. thi ...19957496084
antibacterial effects of some root end filling materials.in addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. this investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, super eba and a mineral trioxide aggregate on nine facultative bacteria streptococcus fecalis, streptococcus mitis, streptococcus mutans, streptococcus salivarius, lactobacillus species, staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, and esc ...19957595152
prosthetic-valve endocarditis caused by peptostreptococcus anaerobius. 19957620041
a numerical taxonomic study of the gram-positive anaerobic cocci.clinical (101) and collection (26) strains of gram-positive anaerobic cocci were examined in conventional tests and pyrolysis mass spectrometry (pms). numerical classifications based upon conventional test reaction patterns (ctrps) and pms showed 27 and 22 clusters, respectively. cross-tabulation of cluster membership in the two classifications showed excellent correlation, with the combined classifications showing clear groups corresponding to the currently recognised species peptostreptococcus ...19957629855
crystalline surface protein of peptostreptococcus anaerobius.the surface ultrastructure of three anaerobic gram-positive cocci frequently encountered in oral infections, peptostreptococcus micros, p. magnus and p. anaerobius, was studied. the type strains of p. micros (dsm 20468) and p. anaerobius (atcc 27337), several clinical isolates of both species and the type strain of p. magnus (dsm 20470) were included. thin-sectioned cells studied by electron microscopy revealed a homogeneous layer outside the peptidoglycan layer in p. anaerobius. in p. micros an ...19957773401
sinusitis and bronchial asthma in children.sinus disease has been assumed to exist in children with respiratory tract allergies. we consecutively evaluated 375 patients (245 male, 130 female) with childhood onset asthma, between 5 and 15 years of age (mean 7.8 years of age). abnormal waters radiographs were found in 205 patients (54.7%) which included mucosal wall thickening greater than 6 mm (67.3%), complete opacification (22.9%) and air-fluid levels (9.8%). the level of immunoglobulin (ig) e was found to be much higher in non-sinusiti ...19957778440
bacteriologic findings in tonsillitis and pericoronitis.bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ...19968816129
emerging resistance of anaerobic bacteria to antimicrobial agents in south korea.in previous studies, bacteroides fragilis group organisms isolated from korean patients were more frequently resistant to various antimicrobial agents, including clindamycin, than were isolates in other countries. a recent report of increased resistance of peptostreptococcus species prompted us to include such isolates in a study of antimicrobial susceptibility. anaerobes isolated in 1994 at a tertiary care hospital in seoul were tested by agar dilution method. none of the b. fragilis group orga ...19968953110
[role of 67 gallium citrate and 99mtc-hmpao-labelled neutrophils in the integrated diagnostic imaging in a case of peptostreptococcus anaerobius hepatic abscess]. 19968966261
an rrna approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination.ruminal amino acid degradation is a nutritionally wasteful process that produces excess ruminal ammonia. monensin inhibited the growth of monensin-sensitive, obligate amino acid-fermenting bacteria and decreased the ruminal ammonia concentrations of cattle. 16s rrna probes indicated that monensin inhibited the growth of peptostreptococcus anaerobius and clostridium sticklandii in the rumen. clostridium aminophilum was monensin sensitive in vitro, but c. aminophilum persisted in the rumen after m ...19968975611
inhibitory effects of metronidazole on anaerobic metabolism of phenylalanine and leucine by peptostreptococcus anaerobius.the metabolism of phenylalanine and leucine by peptostreptococcus anaerobius and the effects of metronidazole on this metabolism were studied. the cells metabolized both phenylalanine and leucine under strict anaerobic conditions and produced hydroxylated products (i.e. phenyllactate from phenylalanine and hydroxyisocaproate from leucine). other products were phenylpropionate, cinnamate and phenylacetate from phenylalanine; isocaproate, alpha-ketoisocaproate and isovalerate from leucine. metroni ...19979069531
bilophila wadsworthiain brain abscess: case report.a case of a patient with a 20-year history of chronic otitis media complicated by cholesteatoma and brain abscess is described. a ct scan with contrast material showed three abscess cavities in the right cerebellar hemisphere. a culture from a specimen of the cholesteatoma yielded a significant amount of growth of bilophila wadsworthia, bacteroides fragilis and prevotella oris and a moderate growth of alpha-streptococci and staphylococcus simulans. from the pus of the brain abscess we also isola ...199716887572
ammonia-hyperproducing bacteria from new zealand ruminants.pasture-grazed dairy cows, deer, and sheep were tested for the presence of ammonia-hyperproducing (hap) bacteria in roll tubes containing a medium in which tryptone and casamino acids were the sole nitrogen and energy sources. colonies able to grow on this medium represented 5.2, 1.3, and 11.6% of the total bacterial counts of dairy cows, deer, and sheep, respectively. a total of 14 morphologically distinct colonies were purified and studied further. restriction fragment length polymorphisms of ...19989572953
bacterial interference in the nasopharynx following antimicrobial therapy of acute otitis media.the effect on the nasopharyngeal bacterial flora of therapy for 10 days with co-amoxiclav or cefprozil was studied in 50 children with acute otitis media. before therapy, potential pathogens (streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis) were isolated in 14 (56%) of those treated with co-amoxiclav and 15 (60%) of those treated with cefprozil. following therapy, the reduction in the number of these pathogens was the same in the two groups. however, differences betwee ...19989598781
aminopeptidase activities in peptostreptococcus spp. are statistically correlated to gelatin hydrolysis.one hundred peptostreptococcus isolates from five species were assessed for their ability to hydrolyze gelatin. most peptostreptococcus magnus (95.8%) and peptostreptococcus micros isolates (79.0%) hydrolyzed gelatin in contrast to peptostreptococcus asaccharolyticus (8.0%), peptostreptococcus anaerobius (10.0%), and peptostreptococcus prevotii isolates (16.7%). gelatin hydrolysis in peptostreptococcus magnus and peptostreptococcus micros isolates correlated (r = 0.80; p = 0.0019) with more amin ...19989606913
development of an anaerobic bacterial leakage model.the majority of bacteria associated with infections of endodontic origin are strict anaerobes. the purpose of this study was to develop an endodontic microleakage model using strict anaerobic bacteria in a two-chamber system. nine species of anaerobic bacteria were tested for viability and detection by either turbidity or color change of the broth. a survey of ph chromogenic substrates revealed that bromcresol purple (ph 5.2 = yellow, ph 6.8 = purple) could be used as a chromogenic indicator to ...19989641124
endodontic pathogens stimulate monocyte chemoattractant protein-1 and interleukin-8 in mononuclear cells.microbial infection of the dental pulp leads to the recruitment of leukocytes and the formation of lesions of endodontic origin. the chemokines interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) are relatively specific chemoattractants for neutrophils and monocytes, respectively. in the present studies, peripheral blood mononuclear cells were stimulated by streptococcus mutants, porphyromonas endodontalis, and peptostreptococcus anaerobius, which are associated with lesions of e ...19989641137
experimental root canal infections in conventional and germ-free mice.a small animal model was evaluated to study the interrelationships between microorganisms after their implantation in root canals (inferior central incisors) using germ-free (gf) and conventional (cv) mice. the selected microorganisms were: porphyromonas endodontalis (atcc 35406), eubacterium lentum (atcc 25559), peptostreptococcus anaerobius (atcc 27337), fusobacterium nucleatum (atcc 10953), escherichia coli (atcc 25922), and enterococcus faecalis (atcc 4083). only p. anaerobius, e. coli, and ...19989693583
in-vitro antibiotic susceptibility and molecular analysis of anaerobic bacteria isolated in cape town, south africa.one hundred and twenty-three anaerobic isolates from cape town, south africa, were tested in vitro against cefoxitin, chloramphenicol, metronidazole, co-amoxiclav, benzylpenicillin and clindamycin. forty-five gram-positive organisms were tested against rp59500 (a streptogramin). resistance in the gram-positive isolates was as follows: of the clostridium perfringens isolates, 4% were resistant to benzylpenicillin and 4% to clindamycin; of the peptostreptococcus anaerobius isolates, 10% were resis ...19989738844
recovery of anaerobic bacteria from a post-traumatic nasal septal abscess. a report of two cases.this report presents 2 children (boys, 11 and 9 years old) who had post-traumatic nasal septal abscesses. cultures for aerobic and anaerobic bacteria revealed peptostreptococcuus magnus and a beta-lactamase-producing prevotella intermedia in the first patient, and peptostreptococcus anaerobius, streptococcus intermedius, and prevotella melaninogenica in the second patient. anaerobic bacteria may play a role in nasal septal abscesses.19989823846
a commensal symbiosis between prevotella bivia and peptostreptococcus anaerobius involves amino acids: potential significance to the pathogenesis of bacterial vaginosis.in both batch and continuous culture, peptostreptococcus anaerobius was able to grow in vaginal defined medium with prevotella bivia, but not in pure culture. growth of p. anaerobius was increased by 238% (p < 0.001) in peptone-supplemented vaginal defined medium conditioned by prior growth of p. bivia. analysis of p. bivia culture supernatants showed a net accumulation of amino acids and subsequent growth of p. anaerobius in the conditioned supernatants resulted generally in amino acid utilizat ...19989879923
total nose reconstruction: a single stage method. 1998105856
[the operative surgery of cancer of the tongue]. 1998106345
chloramphenicol and tetracycline usage in outpatient practice. 1998121377
[c-peptide, igg-insulin antibodies, island cell antibodies and immune response genes in diabetes mellitus]. 1998105488
a report on genetic registers. based on the report of the clinical genetics society working party. 1998106123
fatal leishmaniasis in renal-transport patient. 199988649
a.f.p. and congenital nephrosis finnish type. 199984930
effect on bone of withdrawal of oestrogen therapy. 199989300
new screening test for colorectal cancer developed in canada. 199989207
effect of light on the labeling of optic tectum gangliosides after an intraocular injection of n-[3h]acetylmannosamine. 199985450
[effect of the prolonged administration of chlormadinone acetate on platelet function and blood chemistry with older male persons (author's transl)]. 199991459
plasma 1,25(oh)2 vitamin d concentrations in nutritional osteomalacia. 199989299
[comparison of the cytomorphological classification with the cytochemical differentiation of acute lymphatic leukemia in children].in 100 children with acute lymphatic leukaemia the cytomorphological subclassification of the pathological cell type was made according to mathé and the french-american-british co-operative group (fab). in addition, all cases of leukaemia were differentiated according to their cytochemical type. lymphoblasts from 10 cases of leukaemia could be subclassified immunologically. from 71 children will all the survival rates of those cases of leukaemia subclassified cytomorphologically and the cytochem ...199991554
vitiligo patterns simulating autoimmune and rheumatic diseases.several variants of generalised vitiligo can be recognised by their cutaneous distribution. in some, certain anatomical regions--e.g., hands--are predominantly affected while the cutaneous depigmentation in other variants shows a similar anatomical distribution to that of the internal structures affected in some of the autoimmune and rheumatic diseases. five patients have been seen, and three described by others, with vitiligo affecting skin of eyelids and lower front of neck, reminiscent of the ...199989452
comparative activity of eighteen antimicrobial agents against anaerobic bacteria isolated in south africa.the in vitro activity of 18 antimicrobial agents was determined against 378 anaerobic bacteria isolated in bloemfontein, south africa, during 1996/97. against the gram-positive isolates, mics of penicillin and cefoxitin were >0.5 microg/ml and >16 microg/ml, respectively, for five and three strains of non-perfringens clostridium spp. seventeen peptostreptococcus anaerobius strains were resistant to penicillin (mic > or = 2 microg/ml). all gram-positive anaerobes tested except one peptostreptococ ...199910192714
coaggregation of candida dubliniensis with fusobacterium nucleatum.the binding of microorganisms to each other and oral surfaces contributes to the progression of microbial infections in the oral cavity. candida dubliniensis, a newly characterized species, has been identified in human immunodeficiency virus-seropositive patients and other immunocompromised individuals. c. dubliniensis phenotypically resembles candida albicans in many respects yet can be identified and differentiated as a unique candida species by phenotypic and genetic profiles. the purpose of ...199910203506
in vitro activity of gemifloxacin (sb 265805) against anaerobes.gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...199910471570
acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification.perinatally, and between menarche and menopause, increased levels of estrogen cause large amounts of glycogen to be deposited in the vaginal epithelium. during these times, the anaerobic metabolism of the glycogen, by the epithelial cells themselves and/or by vaginal flora, causes the vagina to become acidic (ph approximately 4). this study was designed to test whether the characteristics of acid production by vaginal flora in vitro can account for vaginal acidity. eight vaginal lactobacillus is ...199910496892
[the range of antagonistic effects of lactobacillus bacterial strains on etiologic agents of bacterial vaginosis].bacterial vaginosis is caused by uncontrolled sequential overgrowth of some anaerobic bacteria: gardnerella vaginalis, prevotella bivia, bacteroides spp., peptostreptococcus spp., mobiluncus sp. usually occurring in stable numbers in the bacterial flora of healthy women. on the other hand, different species of bacteria belonging to the genus lactobacillus, most frequently l. plantarum, l. rhamnosus and l. acidophilus, form a group of aerobic bacteria dominating in the same environment. the diver ...199910865430
features of severe periodontal disease in a teenager with chédiak-higashi syndrome.chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis.200010872965
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