Publications

effects of testosterone, estrogen, and dihydrotestosterone upon aggressive and sexual behavior of female rats.	groups of female tmd rats were treated either with estradiol benzoate (eb), dihydrotestosterone propionate (dhtp), testosterone propionate (tp), eb + dhtp (eb/dhtp), or with oil. these groups of females were tested for social aggression and for masculine and feminine sexual behavior. in addition, patterns of masculine and feminine sexual responses during the aggressive encounters, were investigated. tp-treated females of the same strain were used as opponents in the tests for aggression. in acco ...	1986	3793023
effect of 1% sodium hyaluronate (healon) on a nonregenerating (feline) corneal endothelium.	a series of experiments were performed to investigate the effect of 1% sodium hyaluronate (healon) on the nonregenerating corneal endothelium of the cat. aqueous humor replacement with 1% sodium hyaluronate resulted in mild, transient elevations of intraocular pressure compared to eyes that were injected with balanced salt solution. sodium hyaluronate 1% protected the feline endothelium against cell loss incurred by contact with hyaluronate-coated intraocular lenses compared to endothelial conta ...	1986	3531081
effect of 1% sodium hyaluronate (healon) on a nonregenerating (feline) corneal endothelium.	a series of experiments were performed to investigate the effect of 1% sodium hyaluronate (healon) on the nonregenerating corneal endothelium of the cat. aqueous humor replacement with 1% sodium hyaluronate resulted in mild, transient elevations of intraocular pressure compared to eyes that were injected with balanced salt solution. sodium hyaluronate 1% protected the feline endothelium against cell loss incurred by contact with hyaluronate-coated intraocular lenses compared to endothelial conta ...	1986	3531081
tumor angiogenesis inhibition by prostaglandin synthetase inhibitors.	the release of prostaglandins from tumor cells seems to play an important role in tumor angiogenesis, in which platelet-derived factors may be also included. administration of prostaglandin synthetase inhibitors reduces the growth of both experimental and human malignant tumors. one explanation may be reduced tumor vascularization, as observed in microangiographic studies of experimental transplantable tumors. a similar effect was observed after induced thrombocytopenia. a number of angiogenesis ...	1986	2423014
hepatic tumors in the pediatric age group: a distinctive clinicopathologic spectrum.		1986	215962
public hearings on national health insurance and dentistry's part in them.		1986	1075457
pulp protection in restorative procedures.		1986	1075483
relationship of myocardial metabolism and coronary flow: dependence on extracellular calcium.	the hypothesis that extracellular [ca2+] ([ca2+]e) influences the transmission of vasoregulatory information from the heart to coronary smooth muscle was examined. the isolated, retrogradely perfused rat heart was used to evaluate vascular reactivity to transient infusion of either 0.88 mm amytal (amobarbital), hypoxia (95% n2-5% co2), or 12 microm adenosine in the presence and absence of 2.5 microm verapamil. amytal alone enhanced coronary flow by 78% and lactate efflux by 10-fold, whereas it d ...	1987	2446512
complementation of anti-cea and anti-tag-72 monoclonal antibodies in reactivity to human gastric adenocarcinomas.	monoclonal antibody (mab) b72.3 and mab col-4 are reactive with the high-molecular-weight (mr greater than 10(6] tumor-associated glycoprotein (tag)-72, and the mr 180,000 carcino-embryonic antigen (cea), respectively. antibody competition radioimmunoassays (rias) using 125i-mab b72.3 or 125i-col-4 have demonstrated that each mab also recognizes a distinct antigenic determinant. solid-phase rias using mabs b72.3 and col-4, however, demonstrated similar reactivity for each mab with gastric carcin ...	1987	2447021
onset of steroidogenesis and differentiation of functional lh receptors in rat fetal testicular cultures.	the present study was performed to investigate in vitro the onset of steroidogenesis and the responsiveness to lh in rat fetal testes. the male gonads explanted on days 12.5, 13.5, and 14.5 of gestation in m199 produced testosterone from 15.5 days as is the case in vivo dcamp (1 mm) induced an anticipated steroidogenesis on day 14.5 with secretions of testosterone (0.026 +/- 0.003 ng/gonad/24 h) and progesterone (0.078 +/- 0.005 ng/gonad/24 h), whereas lh (100 ng/ml) has no effect. antiandrogens ...	1987	2823733
light-induced charge separation in photosystem i at low temperature is not influenced by vitamin k-1.	the photoreduction of iron-sulfur centers was studied at low temperature in photosystem i particles from spinach and the cyanobacterium synechocystis 6803, which contain various amounts of vitamin k-1 (recently tentatively identified as the acceptor a1). the irreversible charge separation that was progressively induced at low temperature between p-700 and fa (or fb) by successive laser flashes was studied at 15 k. its maximum amount after a large number of flashes was shown to be fairly independ ...	1987	2823891
initiation codons in mammalian mitochondria: differences in genetic code in the organelle.	the bovine mitochondrial gene products nd2 and nd4, components of nadh dehydrogenase, have been purified from a chloroform/methanol extract of mitochondrial membranes, and the human mitochondrial gene products nd2 and cytochrome b have been obtained by similar procedures. they have been identified by comparison of their amino-terminal protein sequences with those predicted from dna sequences of bovine and human mitochondrial dna. all of the proteins have methionine as their amino-terminal residu ...	1987	2964865
dallas discogram description. a new classification of ct/discography in low-back disorders.	a new classification method for ct/discography was developed. the dallas discogram description (ddd) related five separate categories of information. degeneration and annular disruption were regarded as separate phenomena. additionally, provoked pain response, contrast volume, and miscellaneous information were recorded. discogram findings of 59 patients with low-back and/or leg pain were graded according to the new method and compared with standard methods using routine anteroposterior and late ...	1987	2954226
tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells.	when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ...	1987	3440028
clostridium difficile, sulphasalazine, and ulcerative colitis.	clostridium difficile has been implicated in the relapse of ulcerative colitis. controversy exists over this role and its relationship to sulphasalazine exposure. sixty two of 77 patients with a documented relapse of ulcerative colitis were investigated for the presence of clostridium difficile, or its toxin, prior to hospitalization. there was a low incidence of detection which was related to antibiotic exposure (2/62). sampling during the treatment period showed that the occurrence of clostrid ...	1987	2897683
coronary hemodynamics and myocardial metabolism of lactate, free fatty acids, glucose, and ketones in patients with septic shock.	to investigate disturbances in the coronary circulation and myocardial metabolism during septic shock, we examined coronary sinus blood flow and myocardial substrate extraction in 40 patients with septic shock and 13 control patients. patients with coronary artery disease were excluded from this study. the global hemodynamic pattern of the septic patients was characterized by a lower stroke volume, despite an elevated cardiac index. coronary sinus blood flow was high (187 +/- 47 vs 130 +/- 21 ml ...	1987	3815765
brain l-glutamate decarboxylase: purification and subunit structure.	glutamate decarboxylase (gdcase; l-glutamate-1-carboxy-lyase, ec 4.1.1.15) was purified from whole rat brain approximately equal to 1300-fold to apparent homogeneity with a specific activity of 2.4 units per mg of protein by a combination of column chromatographies on deae-cellulose, hydroxylapatite, and gel filtration, and preparative nondenaturing polyacrylamide gel electrophoresis. the purified preparation contained a single protein band that comigrated with gdcase activity in three diverse a ...	1987	3468504
comparative biodistributions and rates of catabolism of radiolabelled anti-cea monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization.	the rate of catabolism of a radiolabelled monoclonal anti-cea antibody has been compared with that of control igg1 in control nude mice and in mice with cea producing xenografts and antigen negative xenografts. the rate of catabolism of antibody, but not of igg, was increased 3-4 fold in mice with xenografts localizing the antibody, but not in mice with antigen negative tumours. there was no evidence of immune complex formation and/or clearance of antibody from the serum of xenografted mice and ...	1987	3478194
mutagenicity studies by co-cultivation of bronchoalveolar cells and blood lymphocytes with v79 chinese hamster cells.	human bronchoalveolar cells, consisting of approximately 85% pulmonary alveolar macrophages (pams), and peripheral blood lymphocytes isolated from healthy volunteers were investigated for their ability to metabolize 7,8-diol of benzo[a]pyrene (b(a)p). the mutagenicity of reactive metabolites was analyzed by employing a co-cultivation system using v79 chinese hamster cells for the detection of mutations. the metabolic activity of the human cells was compared to pams isolated from rabbits. the num ...	1987	3574323
postpropranolol vasodilation in adrenalectomized glucocorticoid hypertensive rats.	acute beta-adrenoreceptor blockade results in an enhanced blood pressure-lowering effect in glucocorticoid hypertensive rats in the absence of the adrenals. to evaluate the possible mechanism of this enhanced blood pressure-lowering effect, systemic and regional hemodynamics were determined by the radioactive microsphere technique before and after propranolol administration in bilaterally adrenalectomized (ax) and sham-operated (sh) glucocorticoid hypertensive rats. propranolol decreased mean bl ...	1987	3591970
changes in physiological properties of rabbit oviduct by ovarian steroids.	effects of estrogen and progesterone on electrical and mechanical properties of longitudinal smooth muscle cells of the rabbit fallopian tubes were investigated using microelectrode and isometric tension recording methods. in tissues from the estrogen-treated rabbits, there was a decrease in frequency of spontaneous contractions, whereas an increase was seen in tissues from the progesterone-treated rabbits. the membrane potential of smooth muscle cells of the fallopian tube was -58.5 mv. followi ...	1987	3591978
[role of the glossopharyngeal nerve in carotid sinus syncope. experimental evidence in man].		1987	3594498
[role of the glossopharyngeal nerve in carotid sinus syncope. experimental evidence in man].		1987	3594498
effect of altrenogest and lutalyse on parturition control, plasma progesterone, unconjugated estrogen and 13,14-dihydro-15-keto-prostaglandin f2 alpha in sows.	to investigate control of parturition time, 154 sows farrowing 220 litters at three locations were treated with altrenogest and lutalyse (pg). the four treatment groups were: 1) no treatment (control group); 2) an im injection of 15 mg of pg at 1000 on d 111, 112 or 113 of gestation (d 0 = first day of estrus and gestation); 3) altrenogest (20 mg x sow-1 x d-1) fed twice daily for 4 d starting on d 109, 110 or 111; and 4) altrenogest and an injection of pg at 1000 on the day after the last feedi ...	1987	3610870
predation on rhodnius prolixus (hemiptera: reduviidae) by the spider theridion rufipes (araneida: theridiidae).		1987	3546692
calcium-promoted translocation of protein kinase c to synaptic membranes: relation to the phosphorylation of an endogenous substrate (protein f1) involved in synaptic plasticity.	the translocation of protein kinase c between membrane and cytosol has been implicated in several cellular processes (kraft and anderson, 1983; wooten and wrenn, 1984; akers et al., 1985, 1986; hirota et al., 1985; wolf et al., 1986). we desired to identify potential trigger mechanisms underlying the translocation of protein kinase c activity to neural membranes following the synaptic plasticity observed after long-term potentiation (ltp; akers et al., 1986). takai et al. (1979) have suggested a ...	1987	3121805
expression of ras proto-oncogene proteins in normal human tissues.	the expression of ras proto-oncogenes in normal human tissues was studied by immunohistochemical staining and by immunoblotting using monoclonal antibodies. we detected p21ras protein in almost every fetal and adult tissue, but the level varied significantly among cell types. in some cell lineages, immature cells capable of proliferation contain more p21ras than do mature cells. by contrast, certain fully differentiated cells, such as neurons and the epithelial cells of endocrine glands, express ...	1987	3125507
production and characterization of rat monoclonal antibodies with an apparent specificity for certain lung cancers and their potential clinical application.	this paper describes two rat monoclonal antibodies (mabs) (1a6 and 2h7, both igg2a) directed against a human adenocarcinoma of the lung (a549). enzyme-linked immunosorbent assay (elisa) and immunocytochemistry (avidin-biotin-complex method) as the screening of the hybridoma cells revealed that 1a6 and 2h7 mab mainly reacted with adenocarcinoma of the lung, breast and pancreas, and 2h7 mab also recognized large cell carcinoma, epithelia of the lung, trachea, tongue or small cell carcinoma of the ...	1987	3131562
is the horse lung well designed?		1987	3504758
the biochemical basis of niridazole toxicity.		1987	3509430
partial purification and characterization of camp-dependent protein kinase from fasciola hepatica.	three cyclic amp (camp)-dependent protein kinases, designated a1, a2, and b, were isolated from the liver fluke fasciola hepatica using phenyl-sepharose and deae-cellulose chromatography. these enzymes differed with respect to activation by camp and their molecular weights. the half-maximal activation constant for camp-dependent protein kinases a1 and b was 20 nm, while that of a2 was about five-fold higher (110 nm). the estimated molecular weights for camp-dependent protein kinases a1 and a2 (b ...	1987	3037368
[results and prospects for the study of endemic goiter in kazakhstan].	the results of many-year-old research into endemic goiter epidemiology in kazakhstan were summed up. methods for the assessment of potential goiter risk were worked out. multipurpose expedition investigations including mass screenings of the population and animals in all climatic and geographic zones of the republic, an analysis of the concentration of iodine and some other trace elements in the environment and the level of their consumption by man, resulted in compiling a variety of medicogeogr ...	1987	2950511
long-term follow-up of lower lumbar fusion patients.	to determine the long-term effects of lower lumbar fusion, 94 subjects were catalogued from medical records. they had a lumbar arthrodesis at the third lumbar level or below and their operations were performed before 1964. twenty-four were not located and 8 were deceased. sixty-two subjects (72% of available sample) completed a telephone interview; 52 subjects completed a comprehensive low back questionnaire; and 33 subjects returned for physical examination, flexion-extension lateral lumbar spi ...	1987	2954220
redifferentiation and subsequent dedifferentiation of the livers of roosters resulting from acute estrogen challenges.	1. histologic and metabolic changes take place in livers of rooster receiving challenges consisting of acute doses of estrone. 2. during initial growth and redifferentiation livers rapidly increase in size by division of hepatocytes within most lobules, changing from cordlike to acinar configurations. 3. no new lobules appear and degeneration of some cells within lobules takes place even as cell divisions predominate. 4. cells within lobules assume secretory features. 5. vitellogenins, very low ...	1987	2887384
presence of d-aspartate oxidase in rat liver and mouse tissues.	rat liver d-aspartate oxidase activity, which had been reported to be undetectable, was found to be well detectable in dialyzed liver homogenate. the requirements of the enzyme for activity and its sensitivity to inhibitors were identical with the known properties of the enzyme from other sources. we also demonstrated for the first time the presence of the enzyme activity in mouse tissues and some other rat tissues using dialyzed tissue homogenates.	1988	2896518
effects of almitrine on respiration in unanesthetized newborn rabbits.	we previously demonstrated that almitrine, a peripheral chemoreceptor stimulant, increased tidal volume (vt), expired minute ventilation (ve), and respiratory frequency (f) and decreased inspiratory (ti) and expiratory time (te) in sleeping adult cats. we now hypothesized that almitrine would induce an increase in ventilation in a young animal model. respiration was studied by the barometric method in 11 unanesthetized new zealand white rabbit pups between 3 and 6 days of age. recordings were ma ...	1988	2897355
use of restriction fragment length polymorphisms (rflps) to distinguish between nematodes of pathogenic significance.	the availability of restriction fragment length polymorphisms (rflps) would be useful for studying the extent of diversity among morphologically indistinguishable populations of filarial parasites. such polymorphisms may be useful in correlating various physiological and clinical differences with parasite heterogeneity. in order to identify such rflps, we isolated dna from microfilaria of 6 filarial species (acanthocheilonema viteae, brugia malayi, brugia pahangi, dirofilaria immitis, litomosoid ...	1988	2897655
arylsulfatase a from human tissues contains an endo-beta-n-acetylglucosaminidase f-resistant oligosaccharide.	homogeneous arylsulfatase a from human placenta, liver and urine contains two nonidentical subunits of 59 and 54 kda. the two subunits are immunologically identical. the relative amount of low molecular weight subunits is only 20-30% of the total enzyme protein. treatment of the enzyme under various conditions with endo-beta-n-acetylglucosaminidase f results in a decrease in the apparent molecular weight of both subunits by 1-2 kda. a value that corresponds to the loss of a single n-linked oligo ...	1988	2907838
stimulation of growth hormone secretion by central administration of atrial natriuretic polypeptide in the rat.	intracerebroventricular (icv) injection of alpha-human atrial natriuretic polypeptide (alpha hanp) or alpha-rat anp (0.6 and 3 nmol/rat) elicited an increase in plasma gh levels both in conscious freely moving rats and in urethane-anesthetized rats when given at the trough of spontaneous gh secretion. antiserum specific for rat grf did not affect the plasma gh increase induced by icv injection of alpha hanp. intracerebroventricular injection of alpha anp (3 nmol/rat) failed to stimulate gh secre ...	1988	2966063
ultrasound in emergency veterinary medicine.		1988	3051243
[expression of erwinia chrysanthemi ena49 uvr gene in escherichia coli k12 cells].	the uvra gene of erwinia chrysanthemi ena49 similar to uvra gene of escherichia coli k12 has been cloned in vivo in escherichia coli ab1886 uvra6 cells using the plasmid pulb113 (rp4mini mu). the presence of pulb113 carrying uvra gene of erwinia in escherichia coli k12 uvra- cells resulted in suppression of this mutation while uvrb and uvrc are not suppressed by this locus. the genetic control of excision repair of uv-damage in erwinia chrysanthemi ena49 is concluded to be similar to the one in ...	1988	3057355
correlation between increase in ia-bearing macrophages and induction of t cell-dependent antitumor activity by lactobacillus casei in mice.	when lactobacillus casei yit 9018 (lc 9018) or corynebacterium parvum, known to be immunomodulators possessing antitumor activity, were injected i.p. into balb/c mice, peritoneal exudate macrophage ia antigen detected by indirect immunofluorescence method was expressed on their cell surface, but it was not expressed following the injection of 10% proteose peptone, an inflammatory agent, or lactobacillus fermentum yit 0159 (lf 0159), which have no antitumor activity. the percentage and absolute n ...	1988	3133111
coamplification of the hst-1 and int-2 genes in human cancers.	the hst-1 gene, previously designated the hst gene, was identified as a transforming gene by transfection assays of genomic dnas from various types of human cancers. we analyzed for alterations of the hst-1 gene in 18 bladder cancers, 23 renal cell carcinomas and 5 esophageal cancers. although rearrangement of the gene was not detected in any of these samples, amplification of the hst-1 gene was found in 4 samples of tumors, including an invasive bladder cancer, both primary esophageal cancer an ...	1988	3133332
platelet-activating factor and the cardiovascular system: involvement in cardiac anaphylaxis.		1988	3136455
laryngeal reflex mechanism during deglutition--observation of subglottal pressure and afferent discharge.	in this investigation, particular attention was paid to elucidate the laryngeal reflex mechanism of protective closure and the sensory function of the larynx during deglutition. for this purpose, three different experimental procedures were adopted: (1) subglottal pressure of felines was measured during deglutition using a pressure transducer; (2) subglottal pressure of human beings was measured during deglutition using a pressure transducer; and (3) afferent discharges from superior and recurre ...	1988	3147439
ascorbic acid and the eye lens.	exposure of mice to hyperbaric oxygen leads to an inhibition of the mitotic activity in the germinative epithelium of the lens. this is followed by an eventual development of cataracts. cataracts have also been observed in human beings treated with hyperbaric oxygen for different afflictions. the lens damage and cataract formation appears to be due to in situ generation of active radicals and other active species of oxygen. these oxygen derivatives may also contribute to the multifactorial proce ...	1988	3186190
clinical parameters of donkeys before and after trypanosoma vivax infection.		1988	3201022
[qualitative and quantitative parameters of the growth of the skeleton of the extremities of embryos and early fetuses in man].	one hundred and ten human embryos 6-9 weeks of the intrauterine development have been investigated. sequence of anatomical differentiation of the skeleton in the composition of the upper and lower extremities has been stated. quantitative changes of the parameters of each anlage at successive stages of the development are analyzed. equations of regression are obtained, owing to them it is possible to determine age of human embryos and early fetuses in dependence on size of separate fragments of ...	1988	3207399
the human pattern of gyrification in the cerebral cortex.	the degree of cortical folding found in adult human brains has been analyzed using a gyrification index (gi). this parameter permits the description of a mean value for the whole brain, but also a local specific analysis of different brain regions. correlation analyses of the gi with age, body weight, body length, brain weight and volume of the prosencephalon and the cortex show no significant results. gi values do not differ significantly between male and female brains, right and left hemispher ...	1988	3232854
use of haemophilus polysaccharide vaccine in a rural clinic setting.		1988	3258942
correlation with 3h-mpp+ binding sites by debrisoquin and some analogues in mouse brain.		1988	3264919
structural organization of the mouse mitochondrial malate dehydrogenase gene.	structural organization of the mouse mitochondrial malate dehydrogenase (ec 1.1.1.37) gene was determined by analyzing a genomic dna fragment isolated from a cosmid library. the gene is 12,000 base-pairs long and contains nine exons interrupted by eight introns of various sizes. the 5' and 3'-flanking regions, and the exact sizes and boundaries of the exon blocks including the transcription-initiation sites were determined. in the 5'-flanking region, there is neither a tata box nor a caat box. i ...	1988	3379635
binding affinity of serum immunoglobulin g to cardiolipin and other phospholipids in patients with systemic lupus erythematosus and syphilis.	qualitative and quantitative assays for human antibodies to cardiolipin and other phospholipids were used in tests for these reactions in sera from patients with systemic lupus erythematosus (sle) and syphilis. of 22 sle serum samples tested by the qualitative assay, 8 showed positive staining to cardiolipin, phosphatidic acid, and/or phosphatidylserine. all 47 syphilitic sera reacted with these three phospholipids. the apparent affinity of anticardiolipin binding was estimated by normalizing ab ...	1988	3384475
species-specific differences in the toxicity of puromycin towards cultured human and chinese hamster cells.	the toxicity of the protein synthesis inhibitor puromycin towards a number of human and chinese hamster cell lines has been examined. in comparison to cells of human origin, chinese hamster cells exhibited about 25-fold higher resistance towards puromycin. these differences appeared to be species related as all the cell lines from any one species showed similar sensitivity towards puromycin. the incorporation of [3h]leucine in the hamster cell lines was accordingly found to be more resistant to ...	1988	3391265
specific neuro-radiological diagnosis of herpes encephalitis in an animal model.	the potential of utilizing a radio-labelled derivative of the antiviral drug (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu) for the specific, non-invasive, in vivo diagnosis of herpes simplex virus encephalitis (hsve) was investigated in a rat model of the disease. following pharmacological disruption of the blood brain barrier radiolabelled ivdu was administered by intra-carotid injection. brain radioactivity was compared between control and infected animals via gamma camera scintigraphy. after cle ...	1988	3415476
[cytoarchitectonics of the tracheobronchial lymph nodes in rats exposed to carbon disulfide].	cytoarchitectonics and rearrangement of cellular composition in functionally different zones of the rat tracheobronchial lymph nodes have been studied at a short effect of the industrial carbon disulfide vapour at a maximally admissible concentration (mac) (1 mg/m3 and 10 mg/m3), as well as after the effect of mac of carbon disulfide in the inhaled air for 4, 7, 14 days. the inhalation of the carbon disulfide vapours results in certain changes of the cytoarchitectonics of the lymph nodes studied ...	1988	3415483
restriction fragment length polymorphisms detected by anonymous dna probes mapped to defined intervals of human chromosome 16.	three anonymous dna probes ach207, ach224, and ach202, isolated from a flow-purified chromosome 16 library and mapped to defined intervals of human chromosome 16, detected restriction fragment length polymorphisms (rflps). the rflps were of simple two allele types. the ach207 (d16s4) probe detected a taqi and an mspi rflp with polymorphism information content (pic) values of 0.30 and 0.27; the ach224 (d16s5) probe detected an rsai rflp, pic value of 0.34; and the ach202 (d16s4) probe detected an ...	1988	2900214
structure-activity analysis of antagonism of the feedback inhibition of thymidine kinase.	the effects of a variety of 5-, 5'-, and 3'-substituted deoxyuridine derivatives on the cytoplasmic thymidine kinase (ec 2.7.1.21) purified from a human colon carcinoma cell line, hct 116, were determined. of particular interest was elucidation of the structural features important for antagonism of the feedback inhibition of thymidine kinase exerted by thymidine triphosphate. substitutions at the 5-position altered the potency of the 5'-modified compounds. the replacement of the 5-hydrogen with ...	1988	3355601
group a beta-hemolytic streptococci as a cause of bacteremia in children.	the clinical manifestations of patients with group a beta-hemolytic streptococcal (gas) bacteremia presenting to an urban children's hospital were reviewed. group a beta-hemolytic streptococci were isolated from blood cultures from 17 children over a three-year period. systemic illnesses that may have predisposed these children to gas bacteremia were identified in seven patients: preceding varicella infection (four patients) or malignant neoplasm/immunosuppressive therapy (three patients). possi ...	1988	3293424
total and specific ige levels in human hydatid disease determined by enzyme-immunoassay. i. preoperative levels.	the levels of total ige and specific ige in preoperative sera from 29 adult patients with surgically confirmed hydatidosis were studied by means of enzyme-immunoassay. sera from 88 healthy donors were likewise studied. findings showed that in the majority of the hydatidosis cases there was an increase of specific ige and total ige although individual variance was significant. determination of total ige as the only serological diagnostic test is not valid, since 27.5% of patients showed a total i ...	1988	3289333
[determination of salmonella typhimurium antigens in the excreta of children with salmonellosis using the passive hemagglutination reaction].	1,390 samples of different excreta obtained from salmonellosis patients have been tested for the presence of s. typhimurium o- and h-antigens. s. typhimurium antigens, detected with the use of antibody diagnostica, have been found to occur more frequently than s. typhimurium cells. particulate o- and h-antigens capable of agglutinating antibody diagnostica are excreted differently with saliva and urine. salmonella antigens are best detected in feces in the passive hemagglutination test with the ...	1988	3057776
[determination of salmonella typhimurium antigens in the excreta of children with salmonellosis using the passive hemagglutination reaction].	1,390 samples of different excreta obtained from salmonellosis patients have been tested for the presence of s. typhimurium o- and h-antigens. s. typhimurium antigens, detected with the use of antibody diagnostica, have been found to occur more frequently than s. typhimurium cells. particulate o- and h-antigens capable of agglutinating antibody diagnostica are excreted differently with saliva and urine. salmonella antigens are best detected in feces in the passive hemagglutination test with the ...	1988	3057776
the effects of acetylcholine on response properties of cat somatosensory cortical neurons.	1. two-hundred thirty-three single neurons were isolated and studied in somatosensory cortex of cats anesthetized with pentobarbital sodium or urethane. two-hundred and three were studied during iontophoretic administration of acetylcholine (ach), 173 during administration of glutamate, and 24 during administration of atropine. 2. fifty-six percent of the 218 neurons tested responded to somatic stimuli. another 21% did so during glutamate administration. in 11 cases ach iontophoresis uncovered a ...	1988	2897434
retinoid-binding proteins in human oral mucosa.	vitamin a affects epithelial differentiation and growth via at least 3 different binding proteins. the specific delivery of extracellular retinol to target cells is performed by plasma retinol-binding protein (rbp) while cellular retinol- and retinoic acid-binding proteins (crbp and crabp) are implicated in the cellular action of the 2 natural retinoids. we have studied the levels of these 3 binding proteins by gel filtration and polyacrylamide electrophoresis in human oral mucosa, epithelium an ...	1988	2840487
smoking-related alterations in epidermal growth factor and insulin receptors in human placenta.	studies characterized insulin and egf receptors in human placental tissue from smokers and nonsmokers. specific binding of 125i-labeled insulin and egf to placental membranes was not different for nonsmokers compared with smokers. egf and insulin receptor kinases were further studied using a wheat germ agglutinin-purified preparation of solubilized placental membrane proteins. in extracts from the nonsmoker group, egf stimulated the active phosphorylation of mr 170,000 and 140,000 protein bands, ...	1988	2843746
synthesis and characterization of a radiolabelled derivative of the phencyclidine/n-methyl-d-aspartate receptor ligand (+) mk-801 with high specific radioactivity.	a [3h]-labelled derivative of the drug (+)mk-801 with a high specific radioactivity was synthesized by first preparing a tribromo derivative of (+)mk-801 followed by catalytic reduction in the presence of [3h]-gas and subsequent purification of the radioactive product by reversed-phase high performance liquid chromatography (rp-hplc). this resulted in pure (+) [3h]mk-801 with a specific radioactivity of 97 ci/mmol. the (+) [3h]mk-801 was shown to interact with high affinity and selectivity with ...	1988	2845205
naloxone attenuation of sham feeding is modified by manipulation of sucrose concentration.	the time course of sucrose (5, 10 and 20%; w/v) sham feeding was monitored in one hour tests. intake levels increased as a function of concentration. naloxone (1.25 mg/kg, ip) attenuated the sham feeding of 10% sucrose solution in gastric fistulated rats, without affecting initial intake rates. furthermore, after naloxone the intake pattern of 10% sucrose was identical to that for 5% sucrose in untreated rats. in a second test, substitution of 10% sucrose by a 20% solution after 15 min of sham f ...	1988	2853384
mechanism of action of galanin on myenteric neurons.	1. conventional intracellular recording methods were used to investigate the mechanism of action of galanin on electrical behavior of ah/type 2 myenteric neurons in the guinea pig small intestine. 2. the overall action of galanin was inhibitory and consisted of membrane hyperpolarization, decreased input resistance, and suppression of excitability. 3. the action of galanin was on the somatic membrane. there were no effects on spike initiation or propagation velocity in the processes. 4. the reve ...	1988	2459321
hyperstriatal projections to primary visual relays in pigeons: electrophysiological studies.		1988	210884
reconstitution of hormone-sensitive adenylate cyclase activity with resolved components of the enzyme.	adenylate cyclase can be resolved into at least two proteins, a thermolabile, n-ethylmaleimide-sensitive component and a second protein (or proteins) that is more stable to either of these treatments. neither component by itself catalyzes the formation of cyclic amp using mgatp as substrate. however, mixture of the two reconstitutes mgatp-dependent fluoride- and guanyl-5'-yl imidodiphosphate (gpp(nh)p)-stimulatable adenylate cyclase activity. the more stable component can be resolved from the fi ...	1989	210183
neurotological diagnosis of vestibular disorders in children.	the most marked findings in a group of 66 children were a spontaneous nystagmus presenting in 29% of the cases, and an abnormal caloric reaction in 40%. for qualitative and quantitative assessments of the positional test and caloric stimulation, at our university ent department at erlangen we use the positiogram and frequency calorigram described here, which are particularly suitable for follow-up observations and for the purpose of providing an expert medical opinion.	1989	317958
alterations of hepatic sinusoids in liver cirrhosis: their involvement in the pathogenesis of portal hypertension.		1989	2519023
subtype-specific increase in g-protein alpha-subunit mrna by interleukin 1 beta.	the guanine nucleotide regulatory proteins (g-proteins) which are substrates for adp-ribosylation by pertussis toxin (alpha i-1, alpha i-2, alpha i-3 and alpha o) transduce a variety of hormonal signals. endothelial cells express mrna for three alpha i subtypes although the level of alpha i-1 mrna is very low. interleukin 1 beta (il 1 beta), a pleiotropic inflammatory mediator which stimulates a complex series of responses in human endothelial cells leading to increased coagulation and platelet ...	1989	2525485
[pulmonary changes in aids].	we reviewed retrospectively the clinical records of 28 patients with aids staged group iv according to cdc-criteria. among these, 19 had pulmonary disease: most of them (n = 17) had pneumocystis carinii pneumonia (pcp). 12/17 patients with proven pcp displayed typical x-ray findings with diffuse perihilar interstitial infiltration sparing lung periphery. 3/17 had atypical features and 2 normal chest x-ray findings. these data are important to identify patients with pulmonary complications of aid ...	1989	2538872
leishmania donovani: isolation and characterization of sodium stibogluconate (pentostam)-resistant cell lines.	leishmania donovani promastigotes were generated by virtue of their resistance to incrementing concentrations of sodium stibogluconate (pentostam) under completely defined growth conditions. the pent0400 and pent03200 cell lines were isolated after prolonged exposure to 0.4 mg/ml and 3.2 mg/ml pentostam (sb concentration), respectively. whereas the effective concentration of pentostam which inhibited the growth of wild type cells by 50% (ec50 value) was 0.1-0.15 mg/ml, the ec50 values for the pe ...	1989	2546793
characterization of the rela1 mutation and a comparison of rela1 with new rela null alleles in escherichia coli.	the most widely studied "relaxed" mutant of the rela locus, the rela1 allele, is shown here to consist of an is2 insertion between the 85th and 86th codons of the otherwise wild-type rela structural gene, which normally encodes a 743-amino acid (84 kda) protein. the rela protein is a ribosome-dependent atp:gtp (gdp) pyrophosphoryltransferase that is activated during the stringent response to amino acid starvation and thereby occasions the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp). ...	1989	2556396
antisecretory effects of somatostatin and vasopressin in the rat colon descendens in vitro.	the effects of two hormones, vasopressin and somatostatin (som), on ion secretion in rat colon descendens were compared. three modes for induction of epithelial secretion were used: neuronally mediated secretion due to electric field stimulation (efs), ca2+-dependent secretion elicited by carbachol, and camp-dependent secretion evoked either by a receptor-mediated mechanism elicited by vasoactive intestinal peptide (vip) or by a direct activation of the adenylate cyclase by means of forskolin. s ...	1989	2566191
the effect of pinealectomy and jejunal loop diversion on rat small bowel crypt cell kinetics.	previously, it was found that diversion of an isolated loop of jejunum into the colon was associated with a significantly diminished crypt cell proliferation rate in the isolated loop, probably principally because of the diminished amount of nutrient available to the diverted mucosa. it has also been shown previously that removal of the pineal gland is associated with a considerable increase in the jejunal crypt cell mitotic rate. in the present investigation it was found that following pinealec ...	1989	2570492
the corporate medical department and aids.	hiv infection in the workplace reflects the dimensions and distribution of the problem in the community at large. despite this, accommodation of ill employees has been the general pattern and disruptions of any kind the exception. corporate medical departments can be important participants in the clinical and social response to aids. recognition of the illness itself, collaboration in the provision of health services, referral when necessary for treatment and counselling, assistance with medical ...	1989	2597819
alanine is a major end product of metabolism by giardia lamblia: a proton nuclear magnetic resonance study.	1h-nmr spectroscopy was used to monitor the major metabolic end products released by giardia lamblia when maintained anaerobically in culture in diamond's tyi-s-33 medium. spectra were acquired for the cell-free medium and the resonances of metabolites utilised and produced during cell growth identified by the addition of pure compounds and by difference spectroscopy. the major metabolites produced by the parasite were alanine, ethanol and acetate, with increases in concentrations in the media a ...	1989	2615787
biopharmaceutical evaluation of sustained-release ethylcellulose microcapsules containing amoxicillin using beagle dogs.	a beta-lactam antibiotic, amoxicillin, was microencapsulated with ethylcellulose using a solvent evaporation process in liquid paraffin containing sorbitan tristearate as a dispersing agent, and the microcapsules obtained showed first-order drug release. usage of the previous log-log relationship of cefadroxil between in vitro dissolution half-lives (t50) and the experimental release rate constants (k'r) of the drug in vivo, and the nomogram for the design of satisfactory sustained-release prepa ...	1989	2632091
biopharmaceutical evaluation of sustained-release ethylcellulose microcapsules containing amoxicillin using beagle dogs.	a beta-lactam antibiotic, amoxicillin, was microencapsulated with ethylcellulose using a solvent evaporation process in liquid paraffin containing sorbitan tristearate as a dispersing agent, and the microcapsules obtained showed first-order drug release. usage of the previous log-log relationship of cefadroxil between in vitro dissolution half-lives (t50) and the experimental release rate constants (k'r) of the drug in vivo, and the nomogram for the design of satisfactory sustained-release prepa ...	1989	2632091
the protein components of the 12-nanometer microfibrils of elastic and nonelastic tissues.	a procedure has been developed which is much more specific for the solubilization of the elastin-associated microfibrils from fetal bovine nuchal ligament using treatment with reductive saline in place of reductive guanidine hydrochloride buffer. when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, reductive saline extracts were shown to contain only five major protein bands with mrs of 340,000, 78,000, 70,000, 31,000, and 25,000. the 31-kda species was identified immunolo ...	1989	2647740
heterologous signal peptide processing in fusion interferon synthesis by engineered l-forms of proteus mirabilis.	a recombinant dna proteus mirabilis l-form expression system, lvi (pjs127), was used to synthesize human fusion interferon alpha 1 (f-ifn-alpha 1). in the expression plasmid used, the complete coding sequence of ifn-alpha 1 was linked to the streptococcal spea promoter and the 5' end of the spea structural gene including its signal sequence coding region. lvi (pjs127) was capable of complete secretion into the culture medium of biologically active f-ifn-alpha 1 whose identity was confirmed by im ...	1989	2661322
impaired in-vitro proliferation of hemopoietic precursors in hiv-1-infected subjects.	patients with acquired immunodeficiency syndrome (aids) and persistent lymphadenopathy syndrome (las) display significant hematological abnormalities of one or more cell lineages. in order to understand the pathophysiologic mechanisms leading to these abnormalities we studied the proliferation capacity of pluripotent and committed hemopoietic precursors using in-vitro colony assays. anemia, leukopenia and thrombopenia were relatively frequent findings in hiv-infected subjects irrespectively of t ...	1989	2668646
impaired in-vitro proliferation of hemopoietic precursors in hiv-1-infected subjects.	patients with acquired immunodeficiency syndrome (aids) and persistent lymphadenopathy syndrome (las) display significant hematological abnormalities of one or more cell lineages. in order to understand the pathophysiologic mechanisms leading to these abnormalities we studied the proliferation capacity of pluripotent and committed hemopoietic precursors using in-vitro colony assays. anemia, leukopenia and thrombopenia were relatively frequent findings in hiv-infected subjects irrespectively of t ...	1989	2668646
[synthetic cephalosporins. v. synthesis and antibacterial activity of 3-alkylthio-7 beta-[(z)-2-(2-aminothiazol-4-yl)-2-(o-substituted oxyimino)acetamido]cephalosporins and related compounds].	3-alkylthio-7 beta-[(z)-2-(2-aminothiazol-4-yl)-2-(o-substituted oxyimino)acetamido]cephalosporins (6 and 7) and the 3-methoxy analogues (10) were prepared by coupling diphenylmethyl 7-amino-3-alkylthio-3-cephem-4-carboxylate (1 and 2) or diphenylmethyl 7-amino-3-methoxy-3-cephem-4-carboxylate with (z)-2-(2-tritylaminothiazol-4-yl)-2-(o-substituted oxyimino)acetic acid (4), followed by deprotection and subjected to examination of antibacterial activities. the pivaloyloxymethyl esters (8 and 9) o ...	1989	2698417
current management of salivary gland tumors. part 2.	the authors base their treatment of salivary gland malignancies on the size of the primary and the histopathologic diagnosis. group 1 includes smaller tumors in the t1 and t2 classification with cell types that are associated with slow growth. a parotidectomy is usually sufficient therapy for tumors in this group. group 2 contains t1 and 2 tumors with more aggressive behavior. total parotidectomy is indicated here, with postoperative radiotherapy. t3 tumors and patients with nodal metastasis or ...	1989	2701414
a preliminary survey of parasitic infections and nutritional status among school children in riyadh, saudi arabia.	a total of 320 school children in the model institute of the capital in riyadh, were clinically and parasitologically examined. thirty-five (10.94%) had intestinal parasites. entamoeba coli (61.91%) was found more frequently among the commensals, while giardia lamblia (28.57%) was the most common pathogenic parasite. all urines were parasite free. the low infection rate with gardia could be explained by the good physical and nutritional status and better sanitary and living conditions.	1989	2708849
relationship of serum ferritin and iron concentrations and serum total iron-binding capacity to nonheme iron stores in dogs.	the relationships of various iron-related analytes were evaluated in 95 dogs. liver and spleen nonheme iron content was determined coulometrically on acid-digested tissue specimens. serum iron concentration and total iron-binding capacity also were measured coulometrically, whereas serum ferritin concentration was measured by elisa. significant (p less than 0.0002) correlation was found between serum ferritin concentration and nonheme iron stores. significant correlation was not found between no ...	1989	2719382
immunocytochemical localization of adrenodoxin in bovine adrenal cortex by protein a-gold technique.	to investigate the intracellular localization of the enzymes that are involved in steroid hormone synthesis, an immunocytochemical study of the distribution of adrenodoxin in cells of the bovine adrenal cortex was carried out by the post-embedding immunostaining method and by immuno-cryoultramicrotomy in combination with the protein a-gold technique. gold particles were seen on the matrix and the inner membrane of all the mitochondria examined, which have typical vesicular or tubulo-vesicular cr ...	1989	2737927
san people (bushmen) from the central kalahari desert found free of antibodies to hiv-1 and hiv-2.		1989	2754614
critical discussion of carcinogenic risk policy.		1989	2756169
simultaneous measurement of cytosolic free calcium concentration and cell circumference during contraction, both in a single rat cardiomuscular cell, by digital imaging microscopy with indo-1.	we have developed a novel system using digital imaging microscopy with indo-1 to measure the cytosolic free calcium concentration [( ca2+]i). the method is particularly suitable for measuring the rapid change in [ca2+]i in relation to the cell motion. with this system, we made the first successful simultaneous measurement of [ca2+]i and cell circumference during contraction in an electrically stimulated single rat ventricular myocyte. it was found that the level of [ca2+]i was elevated during co ...	1989	2764946
decreased 3 alpha-androstanediol glucuronide levels in plasma and random urines in male pseudohermaphroditism caused by 5 alpha-reductase deficiency.	concentrations of 3 alpha-diol glucuronide (3 alpha-diol g) in plasma and/or random urine samples were determined in seven subjects with familial male pseudohermaphroditism (fmp) due to 5 alpha-reductase deficiency (5 alpha-rd). all subjects were natives of an isolated turkish village with a high incidence of consanguineous marriage. a specific and sensitive antibody to 3 alpha-diol was used for radioimmunoassay of 3 alpha-diol g after hydrolysis and chromatographic purification. the mean plasma ...	1989	2770532
protease nexin-1 complexes and inhibits t cell serine proteinase-1.	the t cell serine proteinase-1 (tsp-1) which most probably is involved in cell killing by cytotoxic t cells is inhibited by protease nexin-1 (pn-1), an extravascular serine protease inhibitor. the inhibition is irreversible and correlates with formation of sds-stable complexes between the two proteins. two distinct species of complexes (91 and 122 kda) are observed upon sds-page analysis of the reacted proteins, indicating that pn-1 is capable of complexing and inhibiting both subunits of the ho ...	1989	2786418
prevention of rectal cancer induced by n-nitrosobis(2-oxopropyl)-amine by exogenous testosterone in mrc rats.	rectal carcinogenicity of n-nitrosobis(2-oxopropyl)amine (bop) in male mrc wistar rats was shown to be inhibited by exogenous testosterone (t) when the hormone was given during, but not after, administration of the carcinogen. this effect was independent of the dose and frequency of bop, which was given either weekly for 20 weeks orally or daily for 3 days subcutaneously. since, except for prostatic cancer, the incidence and the patterns of other bop-induced tumors were not altered by t, this ho ...	1989	2805225
prevention of rectal cancer induced by n-nitrosobis(2-oxopropyl)-amine by exogenous testosterone in mrc rats.	rectal carcinogenicity of n-nitrosobis(2-oxopropyl)amine (bop) in male mrc wistar rats was shown to be inhibited by exogenous testosterone (t) when the hormone was given during, but not after, administration of the carcinogen. this effect was independent of the dose and frequency of bop, which was given either weekly for 20 weeks orally or daily for 3 days subcutaneously. since, except for prostatic cancer, the incidence and the patterns of other bop-induced tumors were not altered by t, this ho ...	1989	2805225
the relation between hospital experience and in-hospital mortality for patients with aids-related pcp.	there is marked debate by physicians and policymakers regarding the creation of regionalized acquired immunodeficiency syndrome (aids) centers. a central issue is whether outcomes of care, particularly mortality, differ as a function of hospital experience with patients with aids. we evaluated the experience of 257 patients with aids and pneumocystis carinii pneumonia treated at 15 california hospitals between october 1986 and october 1987. an overall 15.2% in-hospital mortality rate was observe ...	1989	2785607
purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity.	the central role of cysteine conjugate beta-lyase (beta-lyase) in the bioactivation of nephrotoxic halogenated hydrocarbons and the possibility of human exposure to these chemicals has focused interest on the beta-lyase from human kidney. human kidney tissue was collected as surgical waste material, and subcellular fractions were isolated by differential centrifugation. human beta-lyase activity, determined with s-(2-benzothiazolyl)-l-cysteine (btc) as the substrate, was present in the cytosolic ...	1990	2139845