Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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cyclic nucleotide-dependent regulation of agonist-induced calcium increases in mouse megakaryocytes. | 1. the regulatory effects of cyclic amp and cyclic gmp on adp- and thrombin-induced increases in [ca2+]i were studied in mouse bone marrow megakaryocytes. changes in [ca2+]i were continuously monitored in single fura-2-loaded cells using microspectrofluorometry, and cyclic nucleotides were directly introduced into the single cells using the whole-cell patch-clamp technique. 2. adp increased [ca2+]i in a concentration-dependent fashion, and its threshold concentration was in the order of 0.01 mic ... | 1992 | 1317440 |
structure and function of the cd16:zeta:gamma complex expressed on human natural-killer cells. | natural-killer (nk) cells are large granular lymphocytes involved in host defense against tumor cells and virally-infected cells. in addition to natural cytotoxicity, nk cells can effect antibody-dependent cytotoxicity mediated by cd16 (fc gamma riiia alpha). it has recently been shown that cd16 is associated with disulfide-linked dimers composed of 2 homologous sub-units, zeta and gamma. these transducing molecules are also associated with other multimeric cell-surface receptors such as the t-c ... | 1992 | 1428397 |
genetic mapping of the human tryptophan hydroxylase gene on chromosome 11, using an intronic conformational polymorphism. | the identification of polymorphic alleles at loci coding for functional genes is crucial for genetic association and linkage studies. since the tryptophan hydroxylase (tph) gene codes for the rate-limiting enzyme in the biosynthesis of the neurotransmitter serotonin, it would be advantageous to identify a polymorphism in this gene. by examining introns of the human tph gene by pcr amplification and analysis by the single-strand conformational polymorphism (sscp) technique, an sscp was revealed w ... | 1992 | 1463016 |
re: endoscopic injection of glutaraldehyde cross-linked bovine dermal collagen for correction of vesicoureteral reflux. | 1992 | 1472245 | |
masseter spasm in children and pigs. | 1992 | 1489378 | |
16s rrna phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. | bacterial endosymbionts of insects have long been implicated in the phenomenon of cytoplasmic incompatibility, in which certain crosses between symbiont-infected individuals lead to embryonic death or sex ratio distortion. the taxonomic position of these bacteria has, however, not been known with any certainty. similarly, the relatedness of the bacteria infecting various insect hosts has been unclear. the inability to grow these bacteria on defined cell-free medium has been the major factor unde ... | 1992 | 1557375 |
treatment with digestive agents reveals several glycoconjugates specifically associated with rat neuromuscular junction. | the lectins dba, wga, sba, con a, gs-1, lfa and pna were used to characterize the carbohydrate domains of the rat neuromuscular junction. dba stained only the synaptic domains of muscle surface. all other lectins stained the whole muscle surface but the intensity of staining was stronger at the synaptic regions. however, when sections were treated with several digestive agents prior to lectin application, the lectin staining pattern changed dramatically. collagenase-sensitive glcnac, mannose, si ... | 1992 | 1559843 |
protease composition of exocytosed human skin mast cell protease-proteoglycan complexes. tryptase resides in a complex distinct from chymase and carboxypeptidase. | we characterized the release and the protease composition of high m.w. complexes released from dispersed human skin mast cells, under conditions that did not disrupt the binding of proteases to proteoglycan. the net percent release ratio of tryptase to histamine, after anti-ige and calcium ionophore a23187 stimulation was higher than those for chymase or carboxypeptidase. this was explained by the greater cell association of carboxypeptidase and chymase, compared with tryptase, after mast cell d ... | 1992 | 1560203 |
protease composition of exocytosed human skin mast cell protease-proteoglycan complexes. tryptase resides in a complex distinct from chymase and carboxypeptidase. | we characterized the release and the protease composition of high m.w. complexes released from dispersed human skin mast cells, under conditions that did not disrupt the binding of proteases to proteoglycan. the net percent release ratio of tryptase to histamine, after anti-ige and calcium ionophore a23187 stimulation was higher than those for chymase or carboxypeptidase. this was explained by the greater cell association of carboxypeptidase and chymase, compared with tryptase, after mast cell d ... | 1992 | 1560203 |
long-term activation of protein kinase c by nicotine in bovine adrenal chromaffin cells. | previous results from our laboratory suggest that long-term treatment of primary cultured bovine adrenal medullary (bam) chromaffin cells with nicotine or phorbol 12-myristate 13-acetate, either of which directly activates protein kinase c (pkc), increases the mrna levels encoding catecholamine-synthesizing enzymes and proenkephalin. in the present study, we have examined the effects of nicotine on bam cell pkc activity with special emphasis on long-term effects. nicotine increased particulate p ... | 1992 | 1560224 |
long-term activation of protein kinase c by nicotine in bovine adrenal chromaffin cells. | previous results from our laboratory suggest that long-term treatment of primary cultured bovine adrenal medullary (bam) chromaffin cells with nicotine or phorbol 12-myristate 13-acetate, either of which directly activates protein kinase c (pkc), increases the mrna levels encoding catecholamine-synthesizing enzymes and proenkephalin. in the present study, we have examined the effects of nicotine on bam cell pkc activity with special emphasis on long-term effects. nicotine increased particulate p ... | 1992 | 1560224 |
[infection by human immunodeficiency virus and rheumatic diseases]. | 1992 | 1569794 | |
management of neurologic opportunistic disorders in human immunodeficiency virus infection. | 1992 | 1615237 | |
establishment of stable, cell-mediated immunity that makes "susceptible" mice resistant to leishmania major. | cell-mediated, but not antibody-mediated, immune responses protect humans against certain pathogens that produce chronic diseases such as leishmaniasis. effective vaccination against such pathogens must therefore produce an immunological "imprint" so that stable, cell-mediated immunity is induced in all individuals after natural infection. balb/c mice "innately susceptible" to leishmania major produce antibodies after substantial infection. in the present study, "susceptible" mice injected with ... | 1992 | 1636090 |
time course of cerebral blood flow and histological outcome after focal cerebral ischemia in rats. | the relation between time-dependent changes in cerebral blood flow and the appearance of infarction after focal cerebral ischemia is still a matter for debate. the aim of this study was to measure perfusion after simultaneous occlusions of the left middle cerebral artery and ipsilateral common carotid artery in rats and correlate it with the timing and distribution of histological changes. | 1992 | 1636189 |
a prokaryotic dnaa sequence in drosophila melanogaster: wolbachia infection and cytoplasmic incompatibility among laboratory strains. | using oligonucleotide primers derived from the aligned polypeptide sequences of several prokaryotic dnaa genes, we amplified from drosophila melanogaster dna a 557 bp fragment containing a single open reading frame. the predicted peptide sequence shows a significant similarity to previously characterized protein sequences that are encoded by the dnaa genes of several prokaryotes. the dnaa sequences are also detectable by pcr in dna from drosophila simulans and nasonia vitripennis flies which are ... | 1994 | 7894745 |
taxonomic position of the rickettsiae: current knowledge. | the term rickettsiae initially encompassed all intracellular bacteria. early rickettsial taxonomy was based on a comparison of a few phenotypic characteristics and recently, molecular studies brought new bases for rickettsial taxonomy. all rickettsial species studied so far belong to the alpha and gamma groups of the proteobacteria. ehrlichiae complex groups cowdria ruminantium, anaplasma marginale and wolbachia pipientis and the related parthenogenesis and cytoplasmic incompatibility bacteria, ... | 1994 | 8117465 |
evolution of incompatibility-inducing microbes and their hosts. | in many insect species, males infected with microbes related to wolbachia pipientis are "incompatible" with uninfected females. crosses between infected males and uninfected females produce significantly fewer adult progeny than the other three possible crosses. the incompatibility-inducing microbes are usually maternally transmitted. thus, incompatibility tends to confer a reproductive advantage on infected females in polymorphic populations, allowing these infections to spread. this paper anal ... | 1994 | 28568404 |
phylogenetic analysis of the genus rickettsia by 16s rdna sequencing. | rickettsiae are gram-negative bacteria which multiply only inside host cells and need arthropods either as reservoirs or as vectors. using the polymerase chain reaction and an automated laser fluorescent dna sequencer, we amplified and sequenced the 16s rrna (rdna) of all available bacteria of the genus rickettsia. r. tsutsugamushi remained close to the other bacteria of the genus rickettsia using this technique, contrary to previous conclusions based on the study of the sta-58 protein antigen. ... | 1995 | 8525055 |
characterization of the unlinked 16s rdna and 23s-5s rrna operon of wolbachia pipientis, a prokaryotic parasite of insect gonads. | the rrna-encoding genes (rdnas) have been cloned and characterized from wolbachia pipientis (wp), the gonadial bacteria-like parasite of the mosquito culex pipiens (cp) and the moth ephestia cautella (ec). in wp from both insect species the rdnas are organized in a way which appears to be very unusual. the rrnas are encoded by two unlinked transcription units, each present in a single copy per genome. one contains the 16s rdna only, while the other is an operon encoding both the 23s and 5s rdnas ... | 1995 | 7489921 |
wolbachia pipientis: bacterial density and unidirectional cytoplasmic incompatibility between infected populations of aedes albopictus. | unidirectional cytoplasmic incompatibility is seen when certain wolbachia-infected insect populations are crossed. two hypotheses might explain this phenomenon: superinfections with mutually incompatible strains of wolbachia producing incompatibility when crossed to individuals infected with only a single bacterial strain or, alternatively, a bacterial dosage model, with differences in wolbachia densities responsible for the incompatibility. a quantitative pcr assay was set up as a general metho ... | 1995 | 7498425 |
organization of wolbachia pipientis in the drosophila fertilized egg and embryo revealed by an anti-wolbachia monoclonal antibody. | cytoplasmic incompatibility (ci) in drosophila is related to the presence of wolbachia, an intracellular microorganism found in many species of insects. in order to study the intracellular localization of wolbachia in eggs and embryos, we have purified the bacteria from fly embryos and subsequently generated a monoclonal antibody (mab wol-1) specific for wolbachia. indirect immunofluorescence staining using wol-1 reveals that during mitosis, wolbachia are localized near spindle poles and centros ... | 1995 | 7547474 |
increased male fertility in tribolium confusum beetles after infection with the intracellular parasite wolbachia. | the cytoplasmically inherited microorganism wolbachia pipientis behaves like a sexually selected trait in its host, the flour beetle tribolium confusum, enhancing male fertility at the expense of female fecundity. here we show that infected females have fewer offspring than uninfected females but infected males have a large fertility advantage over uninfected males within multiply-inseminated infected or uninfected females. the male fertility effect accelerates the spread of the wolbachia throug ... | 1995 | 7800041 |
wolbachia pipientis: symbiont or parasite? | 1995 | 15275347 | |
an improved microinjection protocol for the transfer of wolbachia pipientis between infected and uninfected strains of the flour beetle tribolium confusum. | the lethality of halocarbon and other oils to hatching larvae of the flour beetle tribolium confusum limits existing microinjection protocols, because postinjection survivorship is only 5.6% of the eggs injected. we report the development of an oil-free protocol that improves survivorship fivefold. we used this protocol to transfect the cytoplasmic endosymbiont wolbachia pipientis from infected donor eggs to uninfected host eggs and observed reproductive incompatibility in 40% of the surviving, ... | 1996 | 8764685 |
life-history adaptation and reproductive isolation in a grasshopper hybrid zone. | patterns of life-history adaptation and reproductive isolation were investigated in the acridid grasshoppers melanoplus sanguinipes and m. devastator, which hybridize along an altitudinal gradient in the sierra nevada of california. melanoplus sanguinipes females crossed with m. devastator males produced eggs that were approximately half as viable as eggs from other crosses. diminished viability was not attributable either to infection by wolbachia pipientis or to failure of sperm transfer. when ... | 1996 | 28568927 |
in vitro cultivation of wolbachia pipientis in an aedes albopictus cell line. | a continuous cell line, aa23, was established from eggs of a strain of the asian tiger mosquito, aedes albopictus, naturally infected with the intracellular symbiont wolbachia pipientis. the resulting cell line was shown to be persistently infected with the bacterial endosymbiont. treatment with antibiotics cured the cells of the infection. in the course of establishing this cell line it was noticed that rflps in the pcr products of two wolbachia genes from the parental mosquitoes were fixed in ... | 1997 | 9013253 |
phylogeny and potential transmission routes of midgut-associated endosymbionts of tsetse (diptera:glossinidae). | many tsetse species (diptera: glossinidae) harbour two morphologically different intracellular endosymbiotic microorganisms associated with gut tissue: primary (p) and secondary (s) endosymbionts. the p-endosymbionts of tsetse (wigglesworthia glossinidia) are sequestered in specialized epithelial cells, bacteriocytes, which form a structure (bacteriome) in the anterior portion of the gut. phylogenetic characterization of p-endsymbionts from the three subgenera of genus glossina has shown that th ... | 1997 | 9099582 |
ultrastructural differentiation of the genogroups in the genus ehrlichia. | ultrastructural characteristics of 15 strains and isolates of ehrlichiae belonging to three genogroups, or clades of genetically related organisms united in the genera ehrlichia, cowdria, anaplasma, neorickettsia and a strain of wolbachia pipientis which represents a fourth genogroup in this cluster of species, were studied in continuous cell culture or in vivo: e. canis (oklahoma strain and vhe isolate), e. muris (as 145), e. chaffeensis (arkansas, 91he17 and sapulpa), human granulocytic ehrlic ... | 1998 | 9511829 |
overcoming cytoplasmic incompatibility in drosophila. | the endocellular microbe wolbachia pipientis infects a wide variety of invertebrate species, in which its presence is closely linked to a form of reproductive failure termed cytoplasmic incompatibility (ci). ci renders infected males unable to father offspring when mated to uninfected females. because ci can dramatically affect fitness in natural populations, mechanisms that abate ci can have equally large impacts on fitness. we have discovered that repeated copulation by wolbachia-infected male ... | 1998 | 9523438 |
phylogeny and pcr-based classification of wolbachia strains using wsp gene sequences. | wolbachia are a group of intracellular inherited bacteria that infect a wide range of arthropods. they are associated with a number of different reproductive phenotypes in their hosts, such as cytoplasmic incompatibility, parthenogenesis and feminization. while it is known that the bacterial strains responsible for these different host phenotypes form a single clade within the alpha-proteobacteria, until now it has not been possible to resolve the evolutionary relationships between different wol ... | 1998 | 9569669 |
cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont wolbachia pipientis. | the maternally inherited intracellular symbiont wolbachia pipientis is well known for inducing a variety of reproductive abnormalities in the diverse arthropod hosts it infects. it has been implicated in causing cytoplasmic incompatibility, parthenogenesis, and the feminization of genetic males in different hosts. the molecular mechanisms by which this fastidious intracellular bacterium causes these reproductive and developmental abnormalities have not yet been determined. in this paper, we repo ... | 1998 | 9573188 |
evolution of wolbachia pipientis transmission dynamics in insects. | wolbachia pipientis is an intracellular bacterial parasite of arthropods that enhances its transmission by manipulating host reproduction, most commonly by inducing cytoplasmic incompatibility. the discovery of isolates with modified cytoplasmic incompatibility phenotypes and others with novel virulence properties is an indication of the potential breadth of evolutionary strategies employed by wolbachia. | 1999 | 10390640 |
wolbachia pipientis: microbial manipulator of arthropod reproduction. | the alpha-proteobacterium wolbachia pipientis is a very common cytoplasmic symbiont of insects, crustaceans, mites, and filarial nematodes. to enhance its transmission, w. pipientis has evolved a large scale of host manipulations: parthenogenesis induction, feminization, and male killing. w. pipientis's most common effect is a crossing incompatibility between infected males and uninfected females. little is known about the genetics and biochemistry of these symbionts because of their fastidious ... | 1999 | 10547686 |
phylogenetic analysis of the 16s rdna of the cytoplasmic bacterium wolbachia from the novel host folsomia candida (hexapoda, collembola) and its implications for wolbachial taxonomy. | wolbachia pipientis are intracellular, transovarially inherited alpha-proteobacteria in invertebrates. four major wolbachia groups exist: a, b (contained in divergent arthropods), c and d (harbored by nematoda). by means of transmission electron microscopy, we observed wolbachia-like bacteria in a primitive insect, folsomia candida (hexapoda, collembola, isotomidae). 16s rdna analysis proved them to constitute a novel lineage, henceforth named group e, in the wolbachial phylogenetic tree. it sha ... | 1999 | 10556723 |
prenatal diagnosis of congenital adrenal hyperplasia. | 1999 | 91004 | |
[incidence and evaluation of the risk of coronary disease. prospective study in paris]. | the paris prospective study in an epidemiological study of 7,453 middle-aged men born in france, and initially free from ischaemic heart disease. the current mean follow-up time is 4 years. the mean annual incidence is 5.1 per 1000, which is about one half that found in similar american studies. this incidence is related to the cholesterol level, to the blood pressure, to cigarette consumption when the smoke is inhaled, to diabetes mellitus, and to major abnormalities on the electrocardiogram. t ... | 1999 | 407866 |
if i were a dean... | 2000 | 87567 | |
tissue distribution and prevalence of wolbachia infections in tsetse flies, glossina spp. | tsetse flies glossina spp. (diptera: glossinidae) harbor three different symbiotic microorganisms, one being an intracellular rickettsia of the genus wolbachia. this bacterium infects a wide range of arthropods, where it causes a variety of reproductive abnormalities, one of which is termed cytoplasmic incompatibility (ci) that, when expressed, results in embryonic death due to disruptions in fertilization events. we report here that in colonized flies, wolbachia infections can be detected in 10 ... | 2000 | 10759311 |
offsetting effects of wolbachia infection and heat shock on sperm production in drosophila simulans: analyses of fecundity, fertility and accessory gland proteins. | infection in drosophila simulans with the endocellular symbiont wolbachia pipientis results in egg lethality caused by failure to properly initiate diploid development (cytoplasmic incompatibility, ci). the relationship between wolbachia infection and reproductive factors influencing male fitness has not been well examined. here we compare infected and uninfected strains of d. simulans for (1) sperm production, (2) male fertility, and (3) the transfer and processing of two accessory gland protei ... | 2000 | 10790392 |
comparative genomics of mitochondrial dna in drosophila simulans. | the current study compares the nucleotide variation among 22 complete mitochondrial genomes of the three distinct drosophila simulans haplotypes with intron 1 of the alcohol dehydrogenase-related locus. this is the first study to investigate the sequence variation of multiple complete mitochondrial genomes within distinct mitochondrial haplotypes of a single species. patterns of variation suggest distinct forces are influencing the evolution of mitochondrial dna (mtdna) and autosomal dna in d. s ... | 2000 | 10903373 |
expression of cytoplasmic incompatibility in drosophila simulans and its impact on infection frequencies and distribution of wolbachia pipientis. | the aim of this study is to examine the expression of cytoplasmic incompatibility and investigate the distribution and population frequencies of wolbachia pipientis strains in drosophila simulans. nucleotide sequence data from 16s rdna and a wolbachia surface protein coding sequence and cytoplasmic incompatibility assays identify four distinct wolbachia strains: wha, wri, wma, and wau. the levels of cytoplasmic incompatibility between six lines carrying these strains of bacteria and three contro ... | 2000 | 11108593 |
occurrence of novel verrucomicrobial species, endosymbiotic and associated with parthenogenesis in xiphinema americanum-group species (nematoda, longidoridae). | numerous micro-organisms have been described as cytoplasmic symbionts of eukaryotes. many so-called obligate endosymbionts rely exclusively on maternal (vertical or transovarial) transmission to maintain themselves, rendering them dependent on the host sex ratio, which they would tend to manipulate to their own advantage. the latter phenomenon is often associated with the presence of wolbachia pipientis (alpha-proteobacteria) in arthropods and nematodes. a potentially similar situation was disco ... | 2000 | 11155997 |
distribution and diversity of wolbachia infections in southeast asian mosquitoes (diptera: culicidae). | wolbachia are a group of intracellular inherited bacteria that infect a wide range of arthropods. they are associated with a variety of reproductive alterations in their hosts, the best known being cytoplasmic incompatability. the wolbachia pipientis assemblage has been divided into two major groups (a and b) and 12 subgroups. we report herein the first systematic survey of wolbachia in mosquitoes, and the first survey classifying wolbachia infections by subgroup. wolbachia were detected in 28.1 ... | 2000 | 15535575 |
neonatal sacrococcygeal teratoma: multiimaging modality assessment. | neonatal sacrococcygeal teratoma (sct) is a rare and potentially malignant tumor. we report on four cases of neonatal sct, in which ultrasound (us), computed tomography (ct), and magnetic resonance imaging (mri) were used preoperatively to accurately establish the extent of the tumor and its relationship to the surrounding anatomic structures. this approach facilitates complete surgical resection and optimal outcome. | 2000 | 2193140 |
cine mr voiding cystourethrogram in adult normal males. | cine voiding cystourethrogram was performed in 15 normal subjects using t1-weighted turbo flash sequence. it showed the sequence of emptying of bladder, detrusor muscle contraction, opening of the sphincters, and various parts of urethra. it was found to be a good noninvasive test to study the dynamics of lower urinary tract without disturbing the state of the physiology. | 2000 | 1461085 |
histologic fixatives suitable for diagnostic light and electron microscopy. | the merits of formaldehyde, formaldehyde-glutaraldehyde combinations, and glutaraldehyde in phosphate buffers have been compared as fixatives that will give easy and satisfactory preservation of tissues for routine automated histologic processing and yet keep them suitable for electron microscopical studies after prolonged storage at room temperature. we recommend a combination of 4% commercial formaldehyde and 1% glutaraldehyde in a buffer of 176 mosm/liter. tissue sections should not exceed 3 ... | 2001 | 60092 |
the study of enzyme mechanisms by a combination of cosolvent, low-temperature and high-pressure techniques. | 2001 | 95127 | |
in vitro activity of antimicrobial agents against the endosymbiont wolbachia pipientis. | arthropod-transmitted (filarial) nematodes are important causes of disease in humans in tropical countries, yet no safe drug appropriate for mass delivery kills the adult worms. however, most filarial nematodes contain rickettsia-like bacteria of the genus wolbachia, and related bacteria also occur in insects. there is increasing evidence that these bacteria have significant functions in the biology of filarial nematodes. they are thus important targets in the search for antifilarial drugs and e ... | 2001 | 11328780 |
a newly discovered bacterium associated with parthenogenesis and a change in host selection behavior in parasitoid wasps. | the symbiotic bacterium wolbachia pipientis has been considered unique in its ability to cause multiple reproductive anomalies in its arthropod hosts. here we report that an undescribed bacterium is vertically transmitted and associated with thelytokous parthenogenetic reproduction in encarsia, a genus of parasitoid wasps. although wolbachia was found in only one of seven parthenogenetic encarsia populations examined, the "encarsia bacterium" (eb) was found in the other six. among seven sexually ... | 2001 | 11592990 |
wolbachia density and virulence attenuation after transfer into a novel host. | the factors that control replication rate of the intracellular bacterium wolbachia pipientis in its insect hosts are unknown and difficult to explore, given the complex interaction of symbiont and host genotypes. using a strain of wolbachia that is known to over-replicate and shorten the lifespan of its drosophila melanogaster host, we have tracked the evolution of replication control in both somatic and reproductive tissues in a novel host/wolbachia association. after transinfection (the transf ... | 2002 | 11880639 |
electron microscopic and molecular identification of wolbachia endosymbionts from onchocerca lupi: implications for therapy. | it was recently demonstrated that wolbachia intracellular bacteria (alpha 2 proteobacteria, rickettsiales) living in filarial nematodes are obligatory symbionts of their hosts. herein, we report the electron microscopic and 16s ribosomal dna-based (16s rdna) identification of the endobacteria harboring in onchocerca lupi. the worm nodules containing the nematodes were removed from three hungarian dogs naturally infected with o. lupi. wolbachia-like endobacteria were detected by electron microsco ... | 2002 | 11992713 |
a host parasite interaction rescues drosophila oogenesis defects. | the cytoplasmically inherited bacterium wolbachia pipientis is a widespread parasite of arthropods that manipulates the reproductive biology of its hosts, often to their detriment, in order to foster its own transmission through egg cytoplasm. here we report that infection by wolbachia restores fertility to drosophila melanogaster mutant females prevented from making eggs by protein-coding lesions in sex-lethal (sxl), the master regulator of sex determination. suppression of sterility by wolbach ... | 2002 | 12097909 |
host-symbiont conflicts: positive selection on an outer membrane protein of parasitic but not mutualistic rickettsiaceae. | the rickettsiaceae is a family of intracellular bacterial symbionts that includes both vertically transmitted parasites that spread by manipulating the reproduction of their host (wolbachia in arthropods) and horizontally transmitted parasites (represented by cowdria ruminantium), and mutualists (wolbachia pipientis in nematode worms). we have investigated the nature of natural selection acting on an outer membrane protein, the wsp gene in wolbachia and its homologue map1 in cowdria, thought lik ... | 2002 | 12140246 |
within- and between-population variation for wolbachia-induced reproductive incompatibility in a haplodiploid mite. | wolbachia pipientis is a bacterium that induces cytoplasmic incompatibility (ci), the phenomenon in which infected males are reproductively incompatible with uninfected females. ci spreads in a population of hosts because it reduces the fitness of uninfected females relative to infected females. ci encompasses two steps: modification (mod) of sperm of infected males and rescuing (resc) of these chromosomes by wolbachia in the egg. infections associated with ci have mod+ resa+ phenotypes. however ... | 2002 | 12206235 |
lithium and psoriasis. | 2002 | 122448 | |
a study of torsional and angular deflection of endodontic files and reamers. | 2003 | 296261 | |
[angiotomography of supratentorial brain tumors]. | 2003 | 207124 | |
dnaa gene sequences from wolbachia pipientis support subdivision into supergroups and provide no evidence for recombination in the lineages infecting nematodes. | wolbachia pipientis is an intracellular bacterial endosymbiont of arthropods and filarial nematodes. six main supergroups of w. pipientis have been described: supergroups a, b, e, and f encompass arthropod wolbachiae; supergroups c and d encompass nematode wolbachiae. the description of these six supergroups has been based on the analysis of only two genes (ftsz and 16s rdna) and before decisions are taken on the taxonomic status of the six supergroups, analysis of further genes is required. in ... | 2003 | 15270538 |
wolbachia distribution and cytoplasmic incompatibility during sperm development: the cyst as the basic cellular unit of ci expression. | the growth and distribution of the intracellular microbe wolbachia pipientis during spermatogenesis in several different host/symbiont genetic combinations in drosophila melanogaster and drosophila simulans is described. considerable intra- and inter-strain variation in wolbachia density and tissue distribution was observed. wolbachia were found inside spermatocytes and spermatids or within the somatic cyst cells surrounding the germ cells. some strains displayed both tissue distributions. high ... | 2003 | 12559491 |
differences in extent of genetic introgression between sympatric culex pipiens and culex quinquefasciatus (diptera: culicidae) in california and south africa. | comparisons of five morphological characters, 12 enzyme electrophoresis profiles, and wolbachia pipientis infection rates were used to characterize populations of members of the culex pipiens l. complex in california and south africa. in south africa, male phallosome dv/d ratio, male maxillary palp index, branching of siphonal seta 1a, the enzyme locus mdhp-1, and w. pipientis infection rates proved highly diagnostic for separating culex quinquefasciatus from cx. pipiens phenotypes. in johannesb ... | 2003 | 12597651 |
molecular detection of bartonella quintana, b. koehlerae, b. henselae, b. clarridgeiae, rickettsia felis, and wolbachia pipientis in cat fleas, france. | the prevalences of bartonella, rickettsia, and wolbachia were investigated in 309 cat fleas from france by polymerase chain reaction (pcr) assay and sequencing with primers derived from the glta gene for rickettsia, the its and pap31 genes for bartonella, and the 16s rrna gene for anaplasmataceae. positive pcr results were confirmed by using the lightcycler and specific primers for the rompb of rickettsia and glta of bartonella. r. felis was detected in 25 fleas (8.1%), w. pipientis, an insect s ... | 2003 | 12643829 |
wolbachia pipientis growth kinetics and susceptibilities to 13 antibiotics determined by immunofluorescence staining and real-time pcr. | wolbachia spp. are strict intracellular bacteria that infect a wide range of arthropods and filarial nematodes. filarial nematodes are important causes of human diseases. there is increasing evidence that wolbachia spp. influence important functions in the biology of the hosts, specifically, infertility. preliminary experiments with humans and animals have suggested that antibiotics with activity against wolbachia may help to treat filariasis. in this study, we determined using a real-time quant ... | 2003 | 12709338 |
rna polymerase beta-subunit-based phylogeny of ehrlichia spp., anaplasma spp., neorickettsia spp. and wolbachia pipientis. | sequence analysis of rpob, the gene encoding the beta-subunit of rna polymerase, was used in a phylogenetic investigation of nine species from the genera ehrlichia, neorickettsia, wolbachia and anaplasma. the complete nucleotide sequences obtained for anaplasma phagocytophilum (hge agent), ehrlichia chaffeensis, neorickettsia sennetsu, neorickettsia risticii, anaplasma marginale and wolbachia pipientis were amongst the longest rpob sequences in genbank and ranged from 4074 bp for n. sennetsu to ... | 2003 | 12710612 |
endosymbiotic bacterium wolbachia pipientis in synanthropic populations of the mosquito culex pipiens pipiens l. (diptera, culicidae). | 2003 | 12854423 | |
sperm chromatin remodelling and wolbachia-induced cytoplasmic incompatibility in drosophila. | wolbachia pipientis is an obligate bacterial endosymbiont, which has successfully invaded approximately 20% of all insect species by manipulating their normal developmental patterns. wolbachia-induced phenotypes include parthenogenesis, male killing, and, most notably, cytoplasmic incompatibility. in the future these phenotypes might be useful in controlling or modifying insect populations but this will depend on our understanding of the basic molecular processes underlying insect fertilization ... | 2003 | 12897857 |
the distribution and evolutionary history of wolbachia infection in native and introduced populations of the invasive argentine ant (linepithema humile). | wolbachia pipientis is a maternally transmitted bacterium that often alters the life history of its insect host to maximize transmission to subsequent generations. here we report on the frequency and distribution of wolbachia infection in a widespread invasive species, the argentine ant (linepithema humile). we screened 1175 individual argentine ants from 89 nests on five continents and several islands, including numerous locations in both the native (south american) and introduced ranges. we de ... | 2003 | 14629385 |
mitochondrial and wolbachia markers for the sandfly phlebotomus papatasi: little population differentiation between peridomestic sites and gerbil burrows in isfahan province, iran. | in iran, phlebotomus papatasi (scopoli) (diptera: psychodidae) is the main vector of leishmania major yakimoff & schokhor (kinetoplastida: trypanosomatidae), the causative agent of rural zoonotic cutaneous leishmaniasis. this sandfly is abundant both in villages and in the burrows of the main reservoir host, the gerbil rhombomys opimus (licht.) (rodentia: gerbillidae). populations of p. papatasi were sampled from the edges of villages in isfahan province, using cdc miniature light traps in perid ... | 2003 | 14651648 |
culture and phenotypic characterization of a wolbachia pipientis isolate. | the recent isolation of wolbachia pipientis in the continuous cell line aa23, established from eggs of a strain of the asian tiger mosquito aedes albopictus, allowed us to perform extensive characterization of the isolate. bacterial growth could be obtained in c6/36, another a. albopictus cell line, at 28 degrees c and in a human embryonic lung fibroblast monolayer at 28 and 37 degrees c, confirming that its host cell range is broader than was initially thought. the bacteria were best visualized ... | 2003 | 14662922 |
[interactions with the families of down's syndrome infants]. | 2003 | 161789 | |
jaundice. clinical pearls and perils revisited. | i recount some clinical "pearls and perils" to help reassess the contributions of abdominal ultrasound, cholangiography, needle biopsy of the liver, and laparoscopy. abdominal ultrasound demonstrates stones in the gallbladder in approximately 98% of cases, but in only 15% in the common bile duct, whereas computerized tomography scan reveals them in greater than or equal to 50%. on cholangiography a blood clot (in hemobilia) may closely resemble a common duct stone, as may spasm or tumor of the d ... | 2003 | 2958535 |
[transketolase activity as a parameter for the vitamin b1 provision in some dermatoses (herpes zoster, psoriasis, drug eruptions, excema, and urticaria) (author's transl)]. | 2003 | 153861 | |
[the control of renin secretion: experimental data and clinical implications]. | 2004 | 356832 | |
propranolol in acute myocardial infarction. | 2004 | 470741 | |
phylogenomics of the reproductive parasite wolbachia pipientis wmel: a streamlined genome overrun by mobile genetic elements. | the complete sequence of the 1,267,782 bp genome of wolbachia pipientis wmel, an obligate intracellular bacteria of drosophila melanogaster, has been determined. wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. analysis of the wmel genome, in particular phylogenomic comparisons with other intracellular bacteria, has reveale ... | 2004 | 15024419 |
wolbachia pipientis: intracellular infection and pathogenesis in drosophila. | wolbachia pipientis is a vertically transmitted, obligate intracellular symbiont of arthropods. the bacterium is best known for its ability to manipulate host reproductive biology where it can induce cytoplasmic incompatibility, parthenogenesis, feminization and male-killing. in addition to the various reproductive phenotypes it generates through interaction with host reproductive tissue it is also known to infect somatic tissues. however, relatively little is known about the consequences of inf ... | 2004 | 15036143 |
mapping the presence of wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution. | wolbachia pipientis is a bacterial endosymbiont associated with arthropods and filarial nematodes. in filarial nematodes, w. pipientis has been shown to play an important role in the biology of the host and in the immuno-pathology of filariasis. several species of filariae, including the most important parasites of humans and animals (e.g. onchocerca volvulus, wuchereria bancrofti and dirofilaria immitis) have been shown to harbour these bacteria. other filarial species, including an important r ... | 2004 | 15037105 |
the sex ratio distortion in the human head louse is conserved over time. | at the turn of the 19th century the first observations of a female-biased sex ratio in broods and populations of the head louse, pediculus humanus capitis, had been reported. a study by buxton in 1940 on the sex ratio of lice on prisoners in ceylon is still today the subject of reanalyses. this sex ratio distortion had been detected in ten different countries. in the last sixty years no new data have been collected, especially on scalp infestations under economically and socially more developed ... | 2004 | 15140268 |
wolbachia replication and host cell division in aedes albopictus. | wolbachia pipientis is an obligate intracellular endosymbiont of a range of arthropod species. the microbe is best known for its manipulations of host reproduction that include inducing cytoplasmic incompatibility, parthenogenesis, feminization, and male-killing. like other vertically transmitted intracellular symbionts, wolbachia's replication rate must not outpace that of its host cells if it is to remain benign. the mosquito aedes albopictus is naturally infected both singly and doubly with d ... | 2004 | 15297923 |
variable fitness effects of wolbachia infection in drosophila melanogaster. | maternally inherited wolbachia bacteria are extremely widespread among insects and their presence is usually associated with parasitic modifications of host fitness. wolbachia pipientis infects drosophila melanogaster populations from all continents, but their persistence in this species occurs despite any strong parasitic effects. here, we have investigated the symbiosis between wolbachia and d. melanogaster and found that wolbachia infection can have significant survival and fecundity effects. ... | 2004 | 15305172 |
wolbachia pipientis in australian spiders. | wolbachia pipientis is an endosymbiotic bacterium common to arthropods and filarial nematodes. this study presents the first survey and characterization of wolbachia pipientis that infect spiders. all spiders were collected from queensland, australia during 2002-2003 and screened for wolbachia infection using pcr approaches. the wolbachia strains present in the spiders are diverse, paraphyletic, and for the most part closely related to strains that infect insects. we have also identified several ... | 2004 | 15386106 |
wolbachia infection in the newly described ecuadorian sand flea, tunga trimamillata. | wolbachia pipientis is an intracellular endosymbiont producing reproductive alterations in its hosts. this bacterium have been reported in many arthropods and nematodes. by pcr amplification and sequencing of the 16s rdna and ftsz genes we have identified a wolbachia strain in the newly described sand-flea, tunga trimamillata. prevalence of this endosymbiont in the 26 individuals screened is equal to 35%. sympatric and allopatric specimens of the related species tunga penetrans were also analyse ... | 2004 | 15491544 |
genetic diversity of costa rican populations of the rice planthopper tagosodes orizicolus (homoptera: delphacidae). | tagosodes orizicolus (homoptera: delphacidae) is one of the main constraints of the rice production in the neotropics. this planthopper produces severe damages as a phloem feeder, causes mechanical injury during oviposition and vectors the rice hoja blanca virus (rhbv). the main objective of this study was to determine the genetic diversity of t. orizicolus populations from three rice growing regions of costa rica, using rapds. individuals from guanacaste, parrita, san carlos and cali-colombia, ... | 2004 | 17361572 |
diversity of bacteria associated with collembola - a cultivation-independent survey based on pcr-amplified 16s rrna genes. | the bacterial communities found in eight different soil-inhabiting microarthropod species of the class collembola (springtails) were analyzed by analysis of pcr-amplified 16s rrna genes obtained without cultivation from total dna. to characterize the bacteria associated with the parthenogenetically-reproducing folsomia candida, the almost complete 16s rrna genes were amplified with three universal primers, i.e., the forward primer f27 and the reverse primer r1492 or r1525. with the reverse prime ... | 2004 | 19712416 |
[outpatient care in uric acid disorders]. | in 35 patients with uricopathy (arthritis urica, essential hyperuricaemia) the serum uric acid values, consumption of alcohol, overweight, hypertension and liver enzyma under dietary and medicamentous therapy (allopurinol) were investigated. here during an observation lasting 12--48 months allopurinol proved as a well tolerable uricostatic drug which was sufficiently effective also with a considerable exogenic purine application. we only insufficiently succeeded in influencing overweight and alc ... | 2004 | 539007 |
[therapeutic endoscopy in the gastrointestinal tract]. | in selected chapters of the therapeutic endoscopy we tried to represent secure knowledge, actual tendencies and partly future prognoses. hereby completeness cannot be expected. however, it became clear that numerous physical principles became useful for the therapy with the endoscope. chemical techniques, such as the litholysis of gall-stones are only at present used in our field. with all scepticism against the own field of interest one may say that the operative therapeutic endoscopy, as it is ... | 2004 | 539002 |
[dynamics of the humoral immunity indices in children with phenylketonuria against a background of diet therapy and after its discontinuance]. | the paper concerns variations in some parameters of humoral immunity in children with phenylketonuria examined at varying time (in the presence of specific dietetics and after its discontinuation). it is concluded that in children over 5 years of age, the hyperphenylalaninemia newly occurring after discontinuation of dietetics does not exert any material effect on the function of the mature enough immune system. | 2004 | 516615 |
[alcohol intoxication and its differential diagnosis (author's transl)]. | acute alcohol intoxication causes a lot of diagnostic problems. every doctor should be well informed about its differential diagnostic aspects. carelessness concerning diagnosis can be detrimental to the patient and the physician as well. | 2004 | 532410 |
prescribing procrastination. | in his everyday work the family physician sees many patients whose problems have been diagnosed but for whom postponement of an active treatment plan is indicated. the physician must therefore prescribe procrastination in a carefully planned way. i describe some ideas and practical methods for doing this. | 2004 | 529244 |
acute viral respiratory infections in children. | 2004 | 506792 | |
[the late david kohn, m.d]. | 2004 | 321306 | |
a damaging error in australian drinking driver education programmes. | 2004 | 470742 | |
james r. atkinson (january 6, 1920 - february 12, 1978). | 2004 | 356827 | |
significance of epidemiology as viewed by a government scientist. | 2004 | 428572 | |
effects of nifedipine and diltiazem on myocardial ischemia in patients with severe stable angina pectoris treated with nitrates and beta-blockers. | in a randomized, cross-over, double-blind study, the effects of nifedipine were compared with those of diltiazem in 20 patients with severe stable angina pectoris and multivessel coronary artery disease treated with nitrates and beta-blockers. the comparison was performed by bicycle ergometry, clinical evaluation, and ambulatory 24-h ecg monitoring for 7-8 weeks. as compared with placebo, both nifedipine and diltiazem significantly reduced the daily number of anginal attacks and nitroglycerin co ... | 2005 | 1282586 |
[hyperthyroidism during pregnancy]. | the authors review the current knowledge of fetus and woman thyroid physiology during pregnancy. they analyze the abnormalities related to hyperthyroidism, and clinical and therapeutic aspects of thyrotoxicosis; in detail possible fetal complications. they suggest the practical management of thyrotoxicosis during pregnancy. | 2005 | 1287750 |
regulation of glycoconjugate expression on cloned mammalian cells. studies on the growth dependence of membrane-associated/blood group h angigen. | 2005 | 1253842 | |
post-cataract cryptococcal endophthalmitis. | 2005 | 1449785 | |
[hepatotoxicity of cyproterone acetate]. | 2005 | 1410910 | |
[repeated cesarean section. report of 100 cases]. | 2005 | 1175985 |