Publications

physiology and pathophysiology of the colonic circulation.	there is growing evidence that colonic blood flow is controlled by both intrinsic and extrinsic factors. the existence of intrinsic vascular control mechanisms is evidenced by pressure-flow (and oxygen uptake) autoregulation, reactive hyperemia, vascular responses to acute venous hypertension, and a functional hyperemia. although myogenic factors have long been considered to be solely responsible for the intrinsic ability of the colon to regulate its blood flow, recent developments indicate that ...	1986	3536216
epidermal growth factor stimulates (pro-)insulin biosynthesis and 3h-thymidine incorporation in isolated pancreatic rat islets.		1986	3546050
drug metabolism in cirrhosis. selective changes in cytochrome p-450 isozymes in the choline-deficient rat model.	the effect of a choline-deficient diet on microsomal cytochrome p-450 and mixed-function oxidase (mfo) activity was investigated in relation to the development of nutritional cirrhosis. in rats that received the choline-deficient diet for 28 weeks cirrhosis was evident macroscopically and histologically; control rats that received an identical diet supplemented with choline had normal livers. microsomal cytochrome p-450 and cytochrome b5 were reduced in cirrhotic liver to 50% of control levels. ...	1986	3718530
enhancement of affinity to receptors in the esterified glucocorticoid, hydrocortisone 17-butyrate 21-propionate (hbp), in the rat liver.	to investigate the affinity of glucocorticoid (gc) to its receptor, the binding of [3h]hydrocortisone 17-butyrate 21-propionate ([3h]hbp) and [3h]dexamethasone ([3h]dex) in rat liver was analyzed kinetically. scatchard analysis of [3h]hydrocortisone ([3h]hc) binding yielded a curvilinear plot with upward concavity with high and low affinities. the dissociation constant (kd) value of high affinity was 1.9 nm and of low affinity 68.7 nm. a scatchard plot of [3h]hbp binding showed a straight line w ...	1986	3718538
characterization of the subclinical phase of ehrlichiosis in dogs.	prevalence of subclinical ehrlichia canis infection in a mississippi kennel was 53%. most of the dogs probably had been infected for 4 or 5 years. the subclinical phase of the infection was characterized by high antibody titers to e canis (9 of 10 dogs with titers of 1:5, 120), hyperglobulinemia (9 of 10 dogs), thrombocytopenia (5 of 10), absolute lymphocytosis (4 of 10), and absolute neutropenia (3 of 10). the dogs had normal pcv, serum albumin concentrations, and urine protein excretion. findi ...	1986	3733499
nickel binding to the c-terminal tryptic fragment of a peptide from human kidney.	in kidney the nickel ion exists primarily as soluble cytoplasmic complexes. we have recently identified a major component of these complexes in the human kidney as a ni(ii) complex of a low molecular weight anionic peptide (templeton, d.m. and sarkar, b. (1985) biochem. j. 230, 35-42). we have now purified a small amount of this peptide to homogeneity and developed an hplc technique to study its metal-binding properties on sub-nanomole quantities. we are able to demonstrate a binding stoichiomet ...	1986	3768425
a comparison of the effects of climbing fiber deafferentation in adult and weanling rats.	the climbing fiber input to the cerebellar cortex was destroyed using both electrolytic and chemical (3-acetylpyridine) lesions. the long-term effects of climbing fiber deafferentation on the ansiform lobule of weanling and adult rats were examined at both the light and electron microscopic levels. image analysis of golgi-impregnated purkinje cells indicated a significantly lower number of smooth branches and spiny branchlets following climbing fiber deafferentation of both adult and weanling ra ...	1986	3768690
deficient expression of mhc class ii antigens in some cases of human b cell leukaemia.	cells from the peripheral blood of patients with b cell chronic lymphocytic leukaemia (b-cll) and acute lymphoblastic leukaemia (all) were examined for the expression of mhc class ii antigens, using a number of monoclonal antibodies (moab) including l243 (anti-dr) and tu22 (anti-dq). there was wide variation in expression of mhc class ii antigens in cll, both from patient to patient and among cells from the same individual. in a number of subjects a significant proportion of the cells had detect ...	1986	3486060
monoclonal gammopathy associated with naturally occurring canine ehrlichiosis.	clinical, hematologic, and immunologic findings for 14 dogs with ehrlichia canis monoclonal gammopathy were studied retrospectively. epistaxis, anemia, thrombocytopenia, hypoalbuminemia, hypergammaglobulinemia, and proteinuria were documented in the majority of these dogs. the serum protein electrophoresis pattern was characterized by a distinct narrow-base monoclonal spike, by a broad-base monoclonal spike, or by a monoclonal spike superimposed on a polyclonal gammopathy. the monoclonal spike d ...	1987	3506617
fatal disseminated cryptococcosis and concurrent ehrlichiosis in a dog.	laboratory findings in an adult bull terrier presented with a history of anorexia and weight loss included the following: severe anaemia, leukocytosis, neutrophilia, lymphopaenia, thrombocytopaenia, ehrlichia canis morulae in monocytes, hypergammaglo-bulinaemia, a bleeding tendency, icterus and proteinuria. in addition, a high haemobartonella canis parasitaemia, non-encapsulated yeasts on urinalysis and a localised demodex canis infestation were present. treatment for ehrlichiosis was initiated ...	1987	3508465
use of plasmapheresis and chemotherapy for treatment of monoclonal gammopathy associated with ehrlichia canis infection in a dog.	monoclonal gammopathy associated with ehrlichia canis infection was diagnosed in a german shepherd dog. the dog was treated by use of chemotherapy and tetracycline and by plasmapheresis. the dog tolerated plasmapheresis and long-term drug therapy well, and clinical signs resolved over a 90-day period. the monoclonal gammopathy resolved after treatment, but specific antibody to e canis indicated suppression followed by a rebound to the initial high titer.	1987	3583884
unexplained febrile illnesses after exposure to ticks. infection with an ehrlichia?	the ehrlichia are tick-borne rickettsial organisms that cause disease in animals throughout the world but that have been previously recognized as human pathogens only in asia. we have identified six patients with serological evidence of recent infection with an ehrlichia: a fourfold or greater rise or fall in titer to ehrlichia canis. all of the patients reported recent tick bites. rigors, myalgia, headache, nausea, and anorexia were each reported by five patients. fever was present in all patie ...	1987	3586228
distribution of primary afferent fibres from the forelimb of the rat to the upper cervical spinal cord in relation to the location of spinothalamic neuron populations.	half the monosynaptic spinothalamic neurons in the rat spinal cord lie in the first 4 cervical segments, predominantly in the internal basilar column and ventral horn. the present study shows that both these regions receive primary afferents from the forelimb. the superficial dorsal horn receives few, and the region of the lateral cervical nucleus no primary afferents from the brachial plexus. we suggest that internal basilar column cells may represent localised relays concerned with the limbs, ...	1987	3587743
morphological changes in haemocytes of the host, pseudaletia separata, parasitized by microplitis mediator or apanteles kariyai.	the granular cells of the host pseudaletia separata were associated with the initial recognition of foreign materials. when the host was parasitized by microplitis mediator, the filopodial elongation of the haemocytes was strongly suppressed. an inhibition of filopodial elongation was also observed following injection of the calyx and venom fluids of m. mediator. the reduction in the encapsulation capacity of parasitized hosts was confirmed by the experimental injection of sephadex particles and ...	1987	3595946
determination of ivermectin in human plasma and milk by high-performance liquid chromatography with fluorescence detection.		1987	3597637
pathology of brain parenchyma in meningeal carcinomatosis; immunohistochemical study with astroprotein (gfap) and tubulin.	effects of leptomeningeal tumor on the brain parenchyma was studied by the immunohistochemical method with astroprotein (gfap) and tubulin in a rat model of meningeal carcinomatosis. thickening of subpial glial lining (external glial layer) and hypertrophy of subpial astrocytes, detected by the antiserum to gfap, was the early sign of parenchymal involvement. the glial lining was continuous as far as the tumor cells were confined to the subarachnoid space, however, penetration of tumor cells int ...	1987	3598623
human infection with ehrlichia canis.		1987	3627212
[accumulation of messenger rna of the atrial natriuretic factor in the rat left ventricle at the compensatory hypertrophy stage in pressure overload].	the atria produce several peptides that have natriuretic and vasoactive properties, collectively called atrial natriuretic factor. all these peptides share a single messenger ribonucleic acid, the amount of which greatly increases in the rat left ventricle when the latter is submitted to chronic volume overload. using the molecular hybridization technique and a desoxyribonucleic acid probe complementary to the atrial natriuretic factor messenger ribonucleic acid, we now report that a very import ...	1987	2957027
[heterogeneity of purinergic p2 receptors at the level of the caudal artery of the rat and the saphenous vein of the dog].	phentolamine (10(-5) m) and an inhibitor of the lipoxygenase pathway, nordihydroguaiaretic acid (n. d. g. a.; 8 10(-6) m) antagonized the atp induced contraction but not antagonized the utp induced contraction on both rat tail artery and dog saphenous vein. we conclude that the receptors to atp are distinct from receptors to utp and that the p2 purinoceptors are an heterogeneous group.	1987	2957036
[free serotonin levels in injured skin in the early pre- and postmortem periods].		1987	3433317
relationship between htlv-iii neutralizing antibody and clinical status of pediatric acquired immunodeficiency syndrome (aids) and aids-related complex cases.	to investigate a possible protective role of htlv-iii neutralizing antibodies in individuals exposed to the virus, sera of children with acquired immunodeficiency syndrome or acquired immunodeficiency syndrome-related complex were analyzed for neutralizability of htlv-iiib infectivity. twelve pediatric patients (nine acquired immunodeficiency syndrome, three acquired immunodeficiency syndrome-related complex) were clinically stable and had survived more than 2 yr postonset. their predominant cli ...	1987	3474582
clonorchiasis: an update.	the ability of clonorchis sinensis to cause severe hepatobiliary disease is illustrated by the case we have reported, in which biliary obstruction and hepatic fibrosis resulted from the pathologic process associated with chronic clonorchiasis. immunodiagnostic tests are currently limited, and diagnosis, as illustrated by our case, relies on identification of the fluke, usually as the adult or egg stage in clinical specimens. praziquantel has revolutionized therapy of this disease, but surgical d ...	1987	3299730
cytotoxic escherichia coli.		1987	3305326
catecholamine effects on pulmonary blood vessels in strangulation.	guinea pigs were killed by strangulation to investigate the vasomotor of pulmonary congestion by asphyxia. the noradrenaline uptake by the endothelial cells of the pulmonary arteries and capillaries was observed by fluorescence histochemistry, peroxidase-anti-peroxidase (pap) immunocytochemistry, and autoradiography. by radioassay, the volumes of noradrenaline uptake by the pulmonary arteries and capillaries in the strangulation group were at a significantly higher level than in the control grou ...	1987	3321752
chronic cardiac toxicity after inhalation of 1,1,1-trichloroethane.	two patients showed evidence of chronic cardiac toxicity after repeated exposure to 1,1,1-trichloroethane. in both cases there was circumstantial evidence of a deterioration after routine anaesthetic use of the related compound halothane. an adolescent boy who sniffed trichloroethane presented with multiple ventricular arrhythmias during tonsillectomy. follow up showed mild chronic left ventricular impairment. a 54 year old man had repeated industrial exposure to trichloroethane and deteriorated ...	1987	3105712
chronic cardiac toxicity after inhalation of 1,1,1-trichloroethane.	two patients showed evidence of chronic cardiac toxicity after repeated exposure to 1,1,1-trichloroethane. in both cases there was circumstantial evidence of a deterioration after routine anaesthetic use of the related compound halothane. an adolescent boy who sniffed trichloroethane presented with multiple ventricular arrhythmias during tonsillectomy. follow up showed mild chronic left ventricular impairment. a 54 year old man had repeated industrial exposure to trichloroethane and deteriorated ...	1987	3105712
antibiotic sensitivity of pasteurella haemolytica isolated by means of a fibreoptic endoscope from cases of pneumonic pasteurellosis in cattle.	bacterial isolations from tracheal and bronchial washes obtained with the aid of a fibreoptic endoscope were carried out over a 7 month period in a feedlot on calves suffering from acute pneumonic pasteurellosis. pasteurella haemolytica and pseudomonas aeruginosa represented the majority of isolates. antibiotic sensitivities of the pasteurella isolates are reported on.	1987	3127766
human infection with ehrlichia canis, a leukocytic rickettsia.		1987	3029590
reinfection of guinea pigs with herpes simplex virus type 2: failure to establish a second latent ganglionic infection.	guinea pigs were infected with herpes simplex virus type 2 (hsv 2) and at various times thereafter reinfected at a different site with the same or a different strain of hsv 2. viruses were isolated from infected tissues either from the site of primary or secondary infection or from regional ganglia. viral isolates were characterised by dna fingerprinting using restriction endonucleases capable of differentiating between the virus strains used. the second infection resulted in a second site of pe ...	1987	2825951
atrial natriuretic factor: physiologic actions and implications in congestive heart failure.	atrial natriuretic factor (anf) represents a newly recognized hormone of cardiac origin. this peptide is synthesized by the myocardial cells of both atria and released by atrial stretch. the hormone promotes sodium and water excretion by the kidney, inhibits the renin-angiotensin-aldosterone system, and reduces systemic arterial pressure. specific receptors for anf are present in the kidney, adrenal glands, vascular smooth muscle, platelets and central nervous system. congestive heart failure is ...	1987	2978996
two mutant alleles of the insulin receptor gene in a patient with extreme insulin resistance.	insulin receptor complementary dna has been cloned from an insulin-resistant patient with leprechaunism whose receptors exhibited multiple abnormalities in insulin binding. the patient is a compound heterozygote, having inherited two different mutant alleles of the insulin receptor gene. one allele contains a missense mutation encoding the substitution of glutamic acid for lysine at position 460 in the alpha subunit of the receptor. the second allele has a nonsense mutation causing premature cha ...	1988	2834824
two mutant alleles of the insulin receptor gene in a patient with extreme insulin resistance.	insulin receptor complementary dna has been cloned from an insulin-resistant patient with leprechaunism whose receptors exhibited multiple abnormalities in insulin binding. the patient is a compound heterozygote, having inherited two different mutant alleles of the insulin receptor gene. one allele contains a missense mutation encoding the substitution of glutamic acid for lysine at position 460 in the alpha subunit of the receptor. the second allele has a nonsense mutation causing premature cha ...	1988	2834824
effect of dietary fiber on the metabolizable energy of human diets.	two diets consisting of natural foods that differed in fiber content were fed to 12 men for 6 wk each in a crossover design. diet 1 contained 37 g total dietary fiber (tdf) from fruits and vegetables and diet 2 contained 16 g tdf from fruit and vegetable juices in a 2800-kcal menu. seven-day food, urine and fecal composites were made and analyzed for their gross energy, fat, protein and carbohydrate content during the fourth and fifth weeks of each dietary consumption period. the metabolizable e ...	1988	2843615
striatal grafts in rats with unilateral neostriatal lesions--i. ultrastructural evidence of afferent synaptic inputs from the host nigrostriatal pathway.	tyrosine hydroxylase immunocytochemistry, in combination with golgi impregnation, has been used to study the dopaminergic afferent input to striatal suspension grafts implanted into the previously ibotenic acid-lesioned striatum in adult recipient rats. the rats were perfused for combined light- and electron microscopy at 10-11 months after transplantation, at the end of a series of behavioural experiments and a study of in vivo gaba release, reported in the two accompanying papers. a tyrosine h ...	1988	2898109
mutational analysis of domain i of pseudomonas exotoxin. mutations in domain i of pseudomonas exotoxin which reduce cell binding and animal toxicity.	pseudomonas exotoxin (pe) is a single polypeptide chain that contains 613 amino acids and is arranged into three structural domains. domain i is responsible for cell recognition, ii for translocation of pe across membranes and iii for adp ribosylation of elongation factor 2. treatment of pe with reagents that react with lysine residues has been shown to lead to a reduction in cytotoxic activity apparently due to a modification of domain i (pirker, r., fitzgerald, d. j. p., hamilton, t. c., ozols ...	1988	2901411
childhood infection caused by ehrlichia canis or a closely related organism.		1988	3050859
molecular implications of ph (+) myelodysplastic syndrome.	we report a case of 62-year-old japanese male with a myelodysplastic syndrome (mds) with a philadelphia (ph) chromosome. cytogenetic analysis revealed the bone marrow cells to contain a ph chromosome due to t(?;11;22) (?;q11;q11), as well as -5, -7, +8, -12 and an extra ph, in addition to cells with a normal karyotype. molecular analysis using breakpoint cluster region probes (5' bcr and 3' bcr) did not detect a rearrangement within the bcr dna sequences, indicating that the breakpoint at 22q11 ...	1988	3066168
estrogenic control of spermidine/spermine n1-acetyltransferase activity in rat uterus.		1988	3076330
effect of thyroid status and paraventricular area lesions on the release of thyrotropin-releasing hormone and catecholamines into hypophysial portal blood.	trh is a potent stimulator of pituitary tsh release, but its function in the physiological regulation of thyroid activity is still controversial. the purpose of the present study was to investigate trh and catecholamine secretion into hypophysial portal blood of hypothyroid and hyperthyroid rats, and in rats bearing paraventricular area lesions. male rats were made hypothyroid with methimazole (0.05% in drinking water) or hyperthyroid by daily injections with t4 (10 micrograms/100 g bw). untreat ...	1988	3133200
antibodies to ehrlichia canis, ehrlichia platys, and spotted fever group rickettsiae in louisiana dogs.	antibodies to ehrlichia canis, ehrlichia platys, and spotted fever group (sfg) rickettsiae were detected by indirect immunofluorescence in sera from 27 ill individually owned thrombocytopenic dogs (platelet concentrations less than 200,000 platelets/microliters) and 59 healthy kenneled dogs located in southern louisiana. platelet concentrations less than 100,000 platelets/microliters were detected in 63% of ill thrombocytopenic dogs and 6.8% of healthy kennel dogs. one ill thrombocytopenic dog h ...	1988	3146636
nerve growth factor-like activity in the rat pituitary intermediate lobe.	nerve growth factor (ngf)-like immunoreactivity was demonstrated in the rat pituitary intermediate lobe by indirect immunofluorescence method using antisera specific to beta-ngf isolated from adult male mouse submaxillary salivary gland. co-culture of frozen or fresh intermediate lobes with newborn rat superior cervical ganglion resulted in marked fiber growth from the ganglion, which was totally inhibited by ngf antiserum, suggesting the presence in situ and secretion in vitro of biologically a ...	1988	3217050
inhibition of the proliferative response of human b lymphocytes to b cell growth factor by transforming growth factor-beta.	the effects of transforming growth factor-beta (tgf-beta) on the proliferative response of human b cells to the low molecular weight b cell growth factor (bcgf) have been investigated in this study. it was found that tgf-beta, at picomolar concentrations, strongly inhibited the bcgf-induced proliferation of anti-mu chain or staphylococcus aureus cowan i-activated human b cells and also of a bcgf-dependent cell line derived from a human lymphocytic nodular lymphoma. this inhibitory effect was det ...	1988	3257917
transformation of uv-hypersensitive chinese hamster ovary cell mutants with uv-irradiated plasmids.	transfection of uv-hypersensitive, dna repair-deficient chinese hamster ovary (cho) cell lines and parental, repair-proficient cho cells with uv-irradiated phaprt-1 or psv2gpt plasmids resulted in different responses by recipient cell lines to uv damage in transfected dna. unlike results that have been reported for human cells, uv irradiation of transfecting dna did not stimulate the genetic transformation of cho recipient cells. in repair-deficient cho cells, proportionally fewer transformants ...	1988	3257945
diphacinone toxicity, von willebrand's disease, and ehrlichia canis in a dog.		1988	3258688
ehrlichiosis: a cause of bone marrow hypoplasia in humans.	infection with ehrlichia canis should be suspected in patients with fever, headache, malaise, leukopenia, thrombocytopenia, and a history of recent exposure to ticks. the cytopenia is caused by bone marrow hypoplasia which may be severe. the disease may be confused with spotless rocky mountain spotted fever but can be differentiated from this infection serologically with acute and convalescent sea. in humans, recovery has occurred with and without antibiotic therapy. however, prompt antibiotic t ...	1988	3369436
fatal respiratory disease in nilgiri tahr: possibly malignant catarrhal fever.	nilgiri tahr (hemitragus hylocrius) are native to india and are a rare zoo exhibit. this report describes an acute respiratory disease in tahr that caused the death of 15 of 16 animals in an extensive exhibit of about 35 acres where they were housed together with a variety of other exotic species of ruminants. the deaths occurred in two separate outbreaks and were associated with losses from malignant catarrhal fever in other ruminants in the exhibit. the most prominent clinical sign was severe ...	1988	3370557
relative importance of cardiac output and arterial pressure in determining myocardial oxygen consumption and coronary blood flow.	oxygen consumption and coronary blood flow were measured in anesthetized dogs while cardiac work was altered by changing arterial pressure or by opening an aorta to left atrial shunt. when oxygen consumption during pressure work was compared with that during flow work, at a constant heart rate, it was found that flow work increased oxygen consumption as much, or more, than did increasing pressure work. coronary blood flow, but not a-v oxygen difference, was correlated with oxygen consumption. th ...	1988	3370762
occupational factors and the incidence of cancer of the bladder in canada.	during 1979-82, a case-control study of occupational factors and urinary bladder cancer was conducted in edmonton, calgary, toronto, and kingston, canada. a total of 826 histologically verified cases of cancer were individually matched by sex, age, and area of residence to 792 randomly selected population controls. subjects were specifically asked about employment in several industries thought relevant to risk of bladder cancer. information was also obtained on lifelong occupational history, wit ...	1988	3395572
7b2, a new protein secreted by human functionless pituitary tumours, in vitro.	a novel pituitary protein, 7b2, of approximately 180 amino acids has been suggested to colocalise with lh in the pituitary gonadotropes. increased secretion of lh is known to occur in functionless pituitary tumours. we have therefore measured 7b2 immunoreactive equivalents in the 24-h medium of explant pituitary cultures prepared from 17 functionless, 20 somatotropic, 16 prl secreting and 8 corticotropic adenomas. a synthetic fragment corresponding to amino acids 23-39 of 7b2 was used to raise a ...	1988	3400405
stimulation of growth hormone secretion by central administration of atrial natriuretic polypeptide in the rat.	intracerebroventricular (icv) injection of alpha-human atrial natriuretic polypeptide (alpha hanp) or alpha-rat anp (0.6 and 3 nmol/rat) elicited an increase in plasma gh levels both in conscious freely moving rats and in urethane-anesthetized rats when given at the trough of spontaneous gh secretion. antiserum specific for rat grf did not affect the plasma gh increase induced by icv injection of alpha hanp. intracerebroventricular injection of alpha anp (3 nmol/rat) failed to stimulate gh secre ...	1988	2966063
duration of ischemia is vital for collateral development: repeated brief coronary artery occlusions in conscious dogs.	the effects of two types of repeated transient coronary artery occlusions on collateral development were examined in chronically instrumented, conscious dogs. a 2-minute coronary occlusion at 32-minute intervals (group 1, n = 11) or a 15-second occlusion at 4-minute intervals (group 2, n = 7) were repeated day and night without interruption. in both groups, the total duration of coronary occlusions each day was the same (90 minutes). before and after repetitive occlusions of either group, effect ...	1989	2912600
an outbreak of ehrlichiosis in members of an army reserve unit exposed to ticks.	an outbreak of unexplained illness occurred in members of an army reserve unit after field training in an area of new jersey endemic for lyme disease. nine (12%) of the 74 who attended the exercise had serological evidence of ehrlichia infection, defined as a single rise in titer of antibody to ehrlichia canis greater than or equal to 1:160 four weeks after training. two reservists with early serum samples had documented seroconversion, defined by a four-fold or greater increase in titer of anti ...	1989	2915168
glucocorticoid receptor activation in isolated perfused rat hearts.	the formation of unactivated and activated glucocorticoid receptor complexes was studied in intact, isolated, perfused rat hearts in the presence of [3h]triamcinolone acetonide. receptor activation, as quantified by the dna-cellulose-binding assay, began to increase within 30 s of perfusion and reached a final steady-state level (t 1/2 = 4.6 min) with 46% of the steroid-receptor complexes bound to dna-cellulose. with the use of a linear potassium phosphate (kp) gradient (5-400 mm), unactivated r ...	1989	2919654
a serological survey of ehrlichia canis, ehrlichia equi, rickettsia rickettsii, and borrelia burgdorferi in dogs in oklahoma.	serum samples from 259 dogs were tested for antibodies to ehrlichia canis, ehrlichia equi, rickettsia rickettsii, and borrelia burgdorferi using the indirect fluorescent antibody test. the sera were obtained from submissions to the oklahoma animal disease diagnostic laboratory during a 14-month period from june 1986 through july 1987. the rate for positive antibody titers to e. canis was 53%, to e. equi was 33%, to r. rickettsii was 38%, and to b. burgdorferi was 18%. higher percentages of sera ...	1989	2518693
mechanisms and kinetics for platelet and neutrophil localization in immune complex nephritis.	we have previously reported that both neutrophils (pmns) and platelets mediate proteinuria in a model of subendothelial immune complex (ic) nephritis (gn) in the rat. in order to understand the interaction of pmns and platelets in this model, we quantitated the uptake of 111in-labelled platelets in glomeruli and correlated this with the number of pmns observed histologically at 10 and 30 minutes, 1, 4 and 24 hours following induction of gn. platelet accumulation was biphasic with a major peak at ...	1989	2533296
transport of nutrients into the renal brush border membrane vesicles as marker in evaluating the role of antipili antibodies in modulation of ascending pyelonephritis in rats.	the uptake of d-glucose, l-aspartate, l-lysine and l-proline was investigated in renal brush border membrane (bbm) vesicles prepared from control, infected or passively-immunized-infected rats. except l-aspartate, a progressive decrease in the uptake of these nutrients in both infected and immunized-infected groups during the course of infection was observed, but the changes were less apparent in immunized-infected rats than in non-immunized ones. the uptake of l-aspartate was increased in vesic ...	1989	2566554
new cd4(+) cell line susceptible to infection by hiv-1.	infection of a newly described human t lymphoid cell line, cem-cl10, with three different variants of hiv-1 resulted in cytopathic effects followed by cell lysis. following primary lytic infection, proviral dna could not be detected by southern blot analysis in the outgrowth of the surviving cem-cl 10 cells 60 days after initial exposure to hiv-1. these surviving cells could be further grown as a separate line, derived from cem-cl10, and were found to be resistant to subsequent infection by hiv- ...	1989	2570823
different gamma-glutamyl transpeptidase mrnas are expressed in human liver and kidney.	in human, the two subunits of gamma-glutamyl transpeptidase (ggt) arise from a common precursor encoded by a multigene family. until now, a single specific coding sequence for this precursor (type i) has been identified in human placenta and liver. in the present study, we have isolated from a human kidney cdna library, a ggt specific clone (0.8 kb). the sequence of which (type ii) i) covers the carboxy terminal part of the ggt precursor, ii) exhibits 22 point mutations and a 30 bp deletion as c ...	1989	2573352
focus of human parasitism by the brown dog tick, rhipicephalus sanguineus (acari: ixodidae).	throughout most of its range, the brown dog tick, rhipicephalus sanguineus (latreille), prefers dogs as a host, but human bites occasionally occur in the mediterranean region and central america; historically, this species has rarely bitten humans in the united states. a focus of 15 human bite cases by this tick is reported from four air force bases located within 200 miles of each other in north central texas and southwestern oklahoma. the sudden appearance of numerous documented bite cases ind ...	1989	2585458
ehrlichiosis in a dog with seizures and nonregenerative anemia.	ehrlichia canis infection was diagnosed in a dog with a history of seizures and nonregenerative anemia. serologic titer to e canis was greater than 1:100. evaluation of csf revealed a high cell count, high protein concentration, and a positive pandy test result. several mononuclear leukocytes in the csf contained e canis morulae. central nervous system lesions are commonly found on postmortem examination of animals with ehrlichiosis, although clinical reports of neurologic signs attributable to ...	1989	2599963
ehrlichia canis: a tick-borne rickettsial-like infection in humans living in the southeastern united states.	during the past two years, sporadic cases of a rickettsial-like illness were reported in humans living in the southeastern united states. the illness was serologically similar to ehrlichia canis infections in dogs. it resembled spotless rocky mountain spotted fever but was differentiated from this infection serologically with acute and convalescent sera showing increasing titers to ehrlichia canis. e. canis infection should be suspected in patients with fever, headache, malaise, myalgia, gastroi ...	1989	2643878
foreign nations, international organizations, and their impact on health conditions in nicaragua since 1979.	in july 1979, a coalition of social forces in nicaragua, under the leadership of the sandinistas, toppled the discredited 43-year somoza dictatorship. in addition to revolutionary nicaragua's own substantial efforts, since 1979 international forces and developments have had profound impacts on the nation's ambitious social programs. this article investigates the impact of foreign nations and international organizations on nicaragua's health conditions since 1979. given or pledged assistance, for ...	1989	2666319
ehrlichiosis--"spotless spotted fever".	ehrlichia canis, a canine rickettsia, produces an acute infection in humans characterized by thrombocytopenia, leukopenia, and anemia. patients should be closely questioned and examined for tick bites. rash is not prominent, leading to the interesting if somewhat inaccurate label of "spotless spotted fever." the diagnosis is made by clinical presentation, absence of positive lyme disease or rocky mounted spotted fever (rmsf) serology, and positive e. canis titers. tetracyclines are preferred tre ...	1989	2668039
[tumor metastasis and growth factors].	massive investigations are being carried out on growth factors. growth factors are an important determinant for the formation of successive tumor metastasis at target organs. we review the findings, including our data, of growth factors in relation to tumor metastasis.	1989	2679395
induction of acantholysis in organ explant culture by penicillamine and captopril.	pemphigus is an autoimmune disease proved to be mediated by igg autoantibodies. skin lesions clinically and histologically identical to pemphigus may occur in patients receiving penicillamine and captopril, but some of these patients lack circulating or tissue-bound autoantibodies. therefore, we examined the ability of these drugs to produce acantholysis directly in organ explant culture. human skin explants were prepared from split-thickness graft skin from adults and from neonatal foreskins. e ...	1989	2679399
placental transport of calcium and phosphorus in pigs.	the role of vitamin d in regulating placental transport of calcium and phosphate in pigs was studied using the "hannover pig strain" which suffers from pseudo-vitamin d-deficiency rickets, type i. sows and fetuses of normal phenotype (heterozygotes) and of homozygote phenotype which suffered from clinical symptoms of rickets were used. the homozygote animals are devoid of renal 25-cholecalciferol-1-hydroxylase. in the rachitic sows which normally depend on treatment with pharmacological doses of ...	1989	2681666
[clinico-morphological characteristics of extrahepatic pathology caused by the hepatitis b virus].	the current literature and the authors data on the clinics and morphology of the extrahepatic (visceral) pathology provoked by the hepatitis virus are analysed.	1989	2698128
[initial experiences with a monoclonal antibody to the progesterone receptor complex].	today, the immuncytochemical demonstration of estrogen receptors can be regarded as established. recently, a monoclonal antibody (mab) against the progesterone receptor-complex became commercially available. the antibody mi 60-10, developed by milgrom and co-workers, is murine and principally directed against the progesterone receptor of the rabbit, but it also attacks an "epitop" of the human receptor. the western-blot analysis shows the antibody having several specific bands against t-47-d cel ...	1989	2703136
lack of co-transmission of rickettsia conorii and ehrlichia canis in human beings in the south of france.	ehrlichia canis is the agent of canine ehrlichiosis, commonly known as canine tropical pancytopenia. this canine disease is frequent in our area, as is mediterranean spotted fever in humans. recently a few cases of human ehrlichiosis have been described in the usa. the supposed vector of both diseases is rhipicephalus sanguineus. in order to evaluate the co-transmission of these two diseases, we selected 498 human sera positive for r. conorii and tested them for ehrlichia canis. none of them wer ...	1989	2707389
brain biocompatibility of a biodegradable, controlled-release polymer in rats.	we report the biocompatibility in the rat brain of a controlled-release, biodegradable polymer, the polyanhydride poly-[bis(p-carboxyphenoxy)propane-sebacic acid] copolymer (pcpp-sa) in a 20:80 formulation. the biodegradable polyanhydride can be used for drug delivery directly into the brain, circumventing the difficulties posed by the blood-brain barrier and avoiding the consequences of having to administer toxic doses systematically to reach therapeutic doses in the central nervous system. the ...	1989	2708412
infection with ehrlichia canis in a child.		1989	2715894
aseptic meningitis associated with ehrlichia canis infection.		1989	2726324
pharmacokinetic and metabolic studies of high-dose busulphan in adults.	the pharmacokinetics of high-dose busulphan was studied in adult patients with acute myeloblastic leukaemia after oral doses of 1 mg.kg-1 every 6 h for 4 days. the mean steady-state plasma concentration was 1080 ng/ml-1 during the treatment. individual steady-state concentrations after the last dose on average were 32% lower than those predicted from total auc measurements following the first dose. mean elimination half-life in plasma was 2.3 h after the last dose and 3.4 h after the first dose ...	1989	2753072
ehrlichia canis infection in a child.		1989	2771561
the development of analytical models for human trypanosomiasis.	the idea of biological equilibria is presented and applied first to the case of single species populations. difference and differential descriptions of population changes over time are compared and contrasted. the complications that arise when describing interactions between species are then illustrated for the case of vector-borne diseases, such as malaria. a simple model for malaria is explored and later extended to the case of the african trypanosomiases where more than one vertebrate host sp ...	1989	2802828
human ehrlichiosis: prospective active surveillance in febrile hospitalized patients.	between 1 april 1987 and 30 september 1988 prospective active surveillance of human ehrlichiosis was conducted among febrile patients hospitalized in southeast georgia. if disease of the immune system, bacterial infection, pneumonia, or surgical illness was documented by the end of the first full day of hospitalization, the patient was excluded. of 75 patients enrolled in the study, 8 (10.7%) had a fourfold rise or fall in titer to ehrlichia canis (case-patients). the rate was 5.3 cases/100,000 ...	1989	2809255
similarities and differences in the regulation of hepatic cytochrome p-450 enzymes by diabetes and fasting in male rats.	the effects of streptozotocin-induced diabetes and fasting on hepatic cytochrome p-450 enzymes in sexually mature male rats were studied by immunochemical techniques and enzyme assays. the level of cytochrome p-450ac (an acetone/ethanol inducible form), 65 pmol/mg microsomal protein in control rats, increased 4- to 5-fold in diabetic rats and 3- and 5-fold in fasting rats. in contrast, p-450 ut-a (a male specific form) decreased drastically from 295 pmol/mg in the control group to about 10% of t ...	1989	2818618
immunohistologic demonstration of ehrlichia canis.		1990	2165753
ehrlichiosis--a disease of animals and humans.	ehrlichiae are one of several kinds of obligate intracellular bacteria. taxonomically, they are grouped with rickettsiae, but they can be distinguished by their unique tropism for circulating leukocytes. ehrlichia canis causes a pancytopenia in dogs that becomes chronic if untreated. certain breeds develop severe infections, characterized by fever, anorexia, dramatic weight loss, marked pancytopenia, anemia, peripheral edema, and hemorrhage. ehrlichia risticii, a recently discovered species, is ...	1990	2181027
diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity.	human ehrlichiosis, an acute febrile illness caused by ehrlichia canis or a closely related rickettsial organism, was first identified in 1986. from 1986 through 1988, sera from 85 patients demonstrated a fourfold rise or fall in antibody titer to e. canis. seven (22%) of 32 patients initially tested during the first week after onset of illness. 17 (68%) of 25 tested during the second week, and all 18 tested during the third week had titers that exceeded the minimum positive titer of greater tha ...	1990	2192013
changes of ngf level in mouse hypothalamus following intermale aggressive behaviour: biological and immunohistochemical evidence.	nerve growth factor (ngf) immunoreactivity was detected in the hypothalamus of adult male mice. ngf-immunoreactive cell bodies were examined through consecutive brain sections, and it was found that most of the ngf-positive cells were located in the dorsomedial and mediolateral portions of the hypothalamus. tissue culture bioassays showed that hypothalamic extract elicits neurite outgrowth from both chick sensory ganglia and rat superior cervical ganglia neurons, and that these effects are inhib ...	1990	2202329
epidemiologic, clinical, and laboratory findings of human ehrlichiosis in the united states, 1988.	in 1988, the centers for disease control and the oklahoma state department of health identified 40 patients who had a fourfold or greater change in antibody titer in response to ehrlichia canis. the median age of these patients was 42 years, 83% were male, 76% became ill between may and july, and 92% reported recent exposures to ticks. patients resided in or were exposed to ticks in 14 states, including five where ehrlichiosis had not been reported before 1988. thirty-four patients (85%) were ho ...	1990	2214103
the occurrence of soluble and membrane-bound non-specific lipid transfer protein (sterol carrier protein 2) in rat tissues.	the occurrence and subcellular distribution of the non-specific lipid transfer protein (nsl-tp; sterol carrier protein 2) in rat tissues was investigated by the immunoblotting technique using the affinity purified antibody against rat liver nsl-tp. highest levels of the protein were found in the homogenates of liver, lung and adrenals, whereas it could hardly be detected in brain. in other tissues (i.e., testis, kidney, heart and intestine) the protein was present at intermediate concentrations. ...	1990	2223868
the occurrence of soluble and membrane-bound non-specific lipid transfer protein (sterol carrier protein 2) in rat tissues.	the occurrence and subcellular distribution of the non-specific lipid transfer protein (nsl-tp; sterol carrier protein 2) in rat tissues was investigated by the immunoblotting technique using the affinity purified antibody against rat liver nsl-tp. highest levels of the protein were found in the homogenates of liver, lung and adrenals, whereas it could hardly be detected in brain. in other tissues (i.e., testis, kidney, heart and intestine) the protein was present at intermediate concentrations. ...	1990	2223868
molecular cloning of ehrlichia risticii and development of a gene probe for the diagnosis of potomac horse fever.	a gene bank of ehrlichia risticii was constructed in plasmid vector puc13. five clones representing discrete regions of the e. risticii genome were tested for their ability to hybridize specifically to e. risticii dna. none of the clones cross-hybridized with ehrlichia equi dna, whereas four of these clones cross-hybridized with ehrlichia canis and ehrlichia sennetsu dnas. however, one clone carrying a 1-kilobase hindiii fragment of e. risticii dna failed to cross-react with the genomes of e. se ...	1990	2229378
failure to transmit ehrlichia canis (rickettsiales: ehrlichieae) with otobius megnini (acari: argasidae).	an ear tick, otobius megnini (dugès) recovered from a child who had serologic evidence of ehrlichiosis, was examined for ehrlichia species microscopically and by inoculation into a susceptible dog; no evidence of infection was found in the tick. experimental transmission of e. canis by laboratory-reared o. megnini was attempted; neither transstadial nor transovarial transmission occurred.	1990	2231616
concurrent babesiosis and ehrlichiosis in the dog: blood smear examination supplemented by the indirect fluorescent antibody test, using cowdria ruminantium as antigen.	giemsa-stained, peripheral blood smears of 67 dogs, showing clinical signs typical of babesiosis or reminiscent of concurrent babesiosis and ehrlichiosis, were examined for the presence of babesia canis and ehrlichia canis. since cowdria ruminantium cross-reacts with ehrlichia, the sera of these dogs were also subjected to the indirect fluorescent antibody (ifa) test in which c. ruminantium was used as antigen. fifty-five per cent of these dogs had mixed infections of b. canis and e. canis, as j ...	1990	2234860
halophilic proteins and the influence of solvent on protein stabilization.	competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment.	1990	2238041
halophilic proteins and the influence of solvent on protein stabilization.	competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment.	1990	2238041
recombinant dna probe detecting eperythrozoon suis in swine blood.	a genomic library to eperythrozoon suis dna was constructed in lambda gt11, and from this library, e suis clone ksu-2 was identified as a potential diagnostic probe. in hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the ksu-2 probe hybridized strongly with purified e suis organisms and blood samples from splenectomized swine that were parasitized with e suis. however, the probe under stringent conditions did not give radiographic indi ...	1990	2240802
acute upper respiratory tract viral illness and influenza immunization in homes for the elderly.	occupants of 482 long-stay and 33 short-stay beds in 11 leicester city council homes for the elderly were studied during a 30-week period from september 1988 to march 1989 to determine the incidence, aetiology, morbidity, and mortality of acute upper respiratory tract viral infections and the use of influenza vaccine. influenza immunization rates by home ranged from 15.4 to 90% (mean 45%). there were no differences in the distribution of medical conditions by home. the highest immunization rates ...	1990	2249724
stimulatory effects of prostaglandin d2 analogues on adenylate cyclase in rabbit iris-ciliary body membrane fractions.	effects of prostaglandin (pg) d2 analogues on the adenylate cyclase activity in membrane fractions of the iris-ciliary body complex were studied. pgd2 dose-dependently activated the adenylate cyclase with a maximal activity increase of about 60%. the concentration required to cause a half-maximal stimulation (ec50) was about 5 x 10(-7) m. the stimulatory effect of pgd2 was totally dependent on gtp with ec50 for gtp at about 10(-7) m. the rank order of potency of pgd2 analogues for stimulating th ...	1990	2249731
canine ehrlichiosis in connecticut.	the first case of canine ehrlichiosis in connecticut is reported. a female brittany spaniel from milford presented with lethargy, anorexia, fever, petechiae, splenomegaly, thrombocytopenia, anemia, elevated serum alkaline phosphatase, lymphopenia, and hypoalbuminemia. serologic analysis revealed antibodies to ehrlichia canis (titer, 1:2,560). this documents a more northern geographic distribution in the united states for this infectious agent than had previously been suspected.	1990	2312682
epidemiologic and clinical characteristics of persons with serologic evidence of e. canis infection.	the results of a serosurvey of oklahomans for the presence of antibody to ehrlichia canis is reported. paired serum specimens, from patients lacking the serologic criteria for diagnosis of rocky mountain spotted fever (rmsf), were tested. a four-fold increase in e. canis-ifa antibody was found in 16/144 (11 percent) of these paired serum samples. patients with serologic evidence of e. canis infection had a mean age of 34 years, 69 percent were male, and 63 percent lived in a town less than 10,00 ...	1990	2316766
plasma and urinary levels of carnitine in different experimental models of hyperammonemia and the effect of sodium benzoate treatment.	the effect of hyperammonemia on plasma and urinary levels of carnitine was studied in different groups of +/y (normal) and spf/y (chronically hyperammonemic) mice. experimental models of acute and subacute hyperammonemia were prepared in +/y and spf/y mice by the use of ammonium acetate ip injections and arginine-free diets, respectively. in acute hyperammonemia, the plasma levels of both free and acylcarnitines increased significantly whereas acyl/free carnitine ratio was decreased, indicating ...	1990	2346672
the insulin-like growth factor i (igf-i) gene is expressed in chick embryos during early organogenesis.	a definition of the role of igf-i in differentiation and development requires a detailed understanding of its expression and tissue-specific regulation in embryogenesis. standard techniques for analysis of igf-i gene expression are not sufficiently sensitive for studies in early embryos. we have used the highly sensitive polymerase chain reaction (pcr) to study igf-i gene expression in whole chick embryos from the late blastula stage (e0 = laying) through the end of organogenesis (day 8), and in ...	1990	2387267
is androgen-dependent aromatase activity sexually differentiated in the rat and dove preoptic area?	aromatase activity is higher in the male than in the female anterior hypothalamic-preoptic area (poa) in both the avian and the rodent adult brain. this sex difference is abolished after castration of the male and restored by androgen treatment. gonadectomy has no effect on poa aromatase in the female. the aim of this study was to find out whether sex dimorphism in adult poa aromatase is only due to a sex difference in circulating gonadal hormones or dependent upon sexual differentiation of the ...	1990	2394992
"synaptic" ribbons in the pineal gland of the horse.	previous studies on the ultrastructure of the horse pineal gland did not report the presence of "synaptic" ribbons, functionally enigmatic pinealocyte organelles regularly occurring in other mammalian species. the aim of the present study was to reinvestigate the horse pinealocyte in this respect. careful investigations here reported allowed detection of "synaptic" ribbons in the pinealocytes of all the examined animals, although in a relatively low number (5.4 +/- 2.0 per 20,000 microns 2 of pi ...	1990	2395075
structural probing of human lutropin using antibodies raised against synthetic peptides constructed by classical and multiple antigen peptide system approaches.	antibodies were elicited against a synthetic peptide which encompassed two different regions of the human lutropin beta-subunit (hlh-beta). these antibodies were raised against either the peptide which was assembled using a conventional approach and conjugated to the tetanus toxoid, or with the peptide assembled using the multiple antigen peptide system approach. automated simultaneous synthesis of the two forms of the immunizing peptide was successfully achieved. animal injected with the peptid ...	1990	1694262
experimental transmission of granulocytic ehrlichial organisms in dogs.	canine granulocytic ehrlichial organisms were transmitted from an infected dog from missouri to two male, 10-month-old dogs by an intravenous injection of whole blood. physical or behavioral abnormalities were not detected during the 98 days of evaluation other than a mild pyrexia from day 18 to 20. ehrlichial morulae were found in blood granulocytes of dog 1 from day 13 to 44 and of dog 2 from day 14 to 34 with the peak rickettsemia occurring on day 16 for both dogs. by day 21 after inoculaiuon ...	1990	12684945