Publications

cell-mediated immune response to virulent and attenuated anaplasma marginale administered to cattle in live and inactivated forms.	the leukocyte migration-inhibition test (lmit) and lymphocyte transformation of blood leukocytes from cattle exposed to virulent, attenuated, and inactivated preparations of anaplasma marginale served as indices of cell-mediated immunity in bovine anaplasmosis. the lmit response increased markedly in cattle vaccinated with attenuated a marginale or injected with virulent a marginale. cattle inoculated with inactivated anaplasma developed a transient, low-level lmit response. the response to anti ...	1977	842914
finite purification of anaplasma marginale: serologic inactivity of the anaplasma body.	anaplasma bodies finitely purified by affinity chromatography to eliminate all traces of erythrocytic stromata were not agglutinated by serum from calves having acute anaplasmosis. the stromata which were associated with the anaplasma bodies were also finitely purified and agglutinated with such serum. the evidence indicates that the antibody in anaplasmosis that is detected by agglutination tests is directed against the erythrocytic stromata and not against the finitely purified anaplasma bodie ...	1977	879562
cell-mediated immunity related to challenge exposure of cattle inoculated with virulent and attenuated strains of anaplasma marginale.	cattle which were given virulent anaplasma marginale as a premunization procedure or were vaccinated with live attenuated or killed anaplasma preparations were challenge inoculated with virulent a marginale. exposure of cattle to virulent or attenuated a marginale, which induced a strong and lasting cell-mediated response as measured by the leukocyte migration-inhibition test, protected against the development of high parasitemia, anemia, and clinical signs of disease after challenge inoculation ...	1977	911085
survival of aegyptianella pullorum, anaplasma marginale and various parasitic protozoa following prolonged storage in liquid nitrogen.	various protozoa species were examined using in vivo and in vitro methods to determine their ability to survive prolonged periods of storage in liquid nitrogen. the following protozoan species were successfully recovered after they had been cryopreserved for a period over 10 years: trypanosoma lewisi, t. cruzi, t. congolense, t. brucei, t. rhodesiense, t. gambiense, t. evansi, t. equinum, t. equiperdum, leishmania donovani, plasmodium berghei, p. praecox (relictum), babesia rodhaini and b. canis ...	1977	919686
influence of dexamethasone on the recrudescence of anaplasma marginale in splenectomized calves.	dexamethasone was administered at the dose rate of 0.2 mg/kg of body weight to 11 splenectomized anaplasma-carrier calves (groups 1 and 3) on monday, wednesday, and friday for 3 weeks. observations were made on these calves and on 7 nontreated, comparable calves (group 2) to determine the influence of treatment on carrier infections. dexamethasone treatment was associated in every instance with an exacerbation of the anaplasma parasitemia and a decrease in packed red cell volume. the episode of ...	1977	921027
infectivity of anaplasma marginale after ingestion by potential insect vectors.	anaplasma marginale-infected erythrocytes retained viability and infectivity for at least 3 days after ingestion by hippelates pusio loew and up to 2 days after ingestion by species of tabanidae. the organism does not appear to have a developmental sequence in the insect vector.	1977	931144
bovine anaplasmosis transmission studies conducted under controlled natural exposure in a dermacentor andersoni = (venustus) indigenous area of eastern oregon.	in anaplasma marginale transmission studies conducted on the high semi-arid range of eastern oregon during the 1974 and 1975 vector season, a marginale-susceptible calves (principals) were maintained on 2 raised tick-proof platforms. anaplasmosis-susceptible control calves of approximately the same age and latent-infected cows grazed the area surrounding the platforms. one latent-infected steer spent the entire 1975 vector season on a platform with the principals. the 28 principals did not devel ...	1977	557939
the effect of natural exposure to anaplasma and babesia infections on native calves in an endemic area of colombia.	the age at which native calves first became infected with anaplasma marginale and babesia bigemina and the effect of the infections as characterised by anaemia was determined by the biweekly examination of newly born calves on four ranches located in an endemic area of anaplasmosis and babesiosis on the north coast of colombia. all of the calves examined became infected with a. marginale at 4-24 weeks of age and with b. bigemina at 2-34 weeks of age. the mean age at first infection with both hae ...	1977	562010
experimentally induced anaplasmosis in neonatal isohemolytic anemia-recovered calves.	anaplasma marginale infections were induced in 3 calves previously affected with neonatal immunohemolytic anemia (nia). similar infections were induced in 6 splenectomized and 7 intact calves. the response to infection by 3 calves (nia recovered) closely resembled infections seen in splenectomized calves, being markedly more severe than similar infections in intact calves. spleens from 3 (nia recovered) calves after splenectomy were about one-tenth normal size. marked recrudescing anaplasmal inf ...	1977	563204
iatrogenic transmission of anaplasma marginale in beef cattle.		1977	586056
methods of immunoprophylaxis against bovine anaplasmosis with emphasis on use of the attenuated anaplasma marginale vaccine.		1977	596296
[study of cytoplasmic rna in tissues of the rat, regenerating liver and in liver of rats with hepatoma pc-1].	cytoplasmic rna of the rat regenerating liver and liver of rats with hepatoma pc-1 is separated into five fractions on the column of kieselgur with methylated albumin (mak). studies in the nucleotide composition showed that certain fractions of cytoplasm rna are ribosomal and messenger rna. the highest amount of radioactive precursor is incorporated into messenger rna of the rat regenerating liver and liver of the rats with hepatoma pc-1 which is eluated from the column by 0.2% solution of sodiu ...	1977	201068
social organization and food resources availability in primates: a socio-bioenergetic analysis of diet and disease hypotheses.	data obtained during a field study of two species of nonhuman primates (alouatta villosa and ateles geoffroyi) living in the tikal national park in guatemala are used to suggest an answer to the question: to what extent is the existence of a particular form of social organization (group size, structure, and composition) an indication of the amount of energy in the form of food resources available to animals in a particular habitat? seven researchers working in teams spent 2,318 hours in the fiel ...	1977	403771
resistance to anaplasmosis after elimination of latent anaplasma marginale infections.	ten 30-month-old cattle (group 2) were inoculated with anaplasma marginale 6 months after the latent a marginale infection was eliminated by oral chlortetracycline therapy. in addition, 4 cattle (group 1) with no record of infection and 4 cattle (group 3) of equivalent age previously vaccinated with a killed antigen were similarly inoculated. moderate to severe clinical signs of acute anaplasmosis occurred in the group 1 (previously uninfected) cattle, whereas only 1 of the chlortetracycline-tre ...	1977	557302
locomotion of human lymphoid cells. i. effect of culture and con a on t and non-t lymphocytes.		1977	303147
electrophoresis of lysozyme into microscoccus-containing agarose gel: quantitative and analytical applications.	electrophoresis of lysozyme into agarose gel containing micrococcus lysodeikticus causes lysis of the microorganism, allowing the development of two methods, one for quantitation ("lyso-rocket electrophoresis") and the other for electrophoretic characterization ("crossed lyso-electrophoresis") of lysozyme. the lyso-rocket technique provides an alternative to the method currently used for quantitative assay. by the use of crossed lyso-electrophoresis we have provided evidence, for the first time, ...	1977	265197
evidence for the maintenance of motoneurone properties by muscle activity.	1. electrophysiological properties of soleus motoneurones in adult cats were examined with intracellular electrodes following alterations of activity of the soleus muscle induced by transection of the thoracic spinal cord or by conduction block of the muscle nerve with tetrodotoxin (ttx) cuffs. attempts were also made to maintain muscle activity by daily stimulation of the maintain muscle activity by daily stimulation of the peripheral nerve. 2. within 8 days after transection of the thoracic co ...	1978	279668
[characteristics of sepiapterin reductase from tawa and yoshida sarcomas (author's transl)].		1978	228781
cyclic thrombocytopenia induced by a rickettsia-like agent in dogs.	hematologic manifestations and the ultrastructure of a platelet-specific microorganism isolated from a dog in florida were studied. the agent was readily transmitted experimentally to adult dogs by intravenous inoculation with infected blood. parasitemias and concomitant thrombocytopenias were cyclic in that both recurred within relatively constant periods of one to two weeks following experimental infections. hemorrhage was not a manifestation of the disease even though thrombocytopenias were s ...	1978	627738
efficacy of attenuated anaplasma marginale vaccine under laboratory and field conditions in colombia.	four-month-old holstein-friesian calves were inoculated with 3 different doses (1, 2, and 3 ml) of attenuated anaplasma marginale vaccine. vaccinated calves showed mild anaplasma parasitemia, slight decrease in packed cell volume, low serologic conversion, and no clinical illness. an artificial challenge exposure of vaccinated and unvaccinated calves with virulent colombian a marginale showed that the vaccine provided protection against clinical signs of the disease, including parasitemia and an ...	1978	629456
fluorescein diacetate staining of anaplasma marginale as a possible measure of viability.	fluorescein diacetate viability-staining technique was applied to anaplasma marginale. marginal bodies took up the stain and became fluorescent. when infected red blood cells were incubated at 55 c, the number of fluorescent anaplasma decreased at a rate similar to the known loss of viability.	1978	629472
serologic and hematologic response of rabbits to anaplasma marginale-infected bovine erythrocytes.	new zeland white rabbits inoculated with anaplasma marginale-infected bovine erythrocytes produced specific humoral antibodies to this parasite. specific antibody production as measured by the card and complement-fixation (cf) tests was maximal in 7 to 21 days after inoculation; however, none of the rabbits became infected. geometric mean cf titers of rabbits inoculated with rbc from cattle with acute anaplasmosis were significantly higher (p less than 0.001) than those of rabbits given rbc from ...	1978	646203
attempted transmission of anaplasma marginale by haemaphysalis longicornis.		1978	655986
anaplasmosis: a regional serologic survey and oral antibiotic therapy in infected herds.	a test and treatment program was conducted in northern idaho and southeastern washington to detect bovine reactors to anaplasma marginale and to achieve anaplasmosis-free status in the herds tested. of 3,920 cattle tested, 214 (5.5%) were reactors; 110 of the reactors (from 7 herds) were tested after being treated orally for 45 days with chlortetracycline or oxytetracycline at a calculated daily dosage of 11 mg/kg of body weight, and all were seronegative. two reactors refused to eat the medicat ...	1978	659312
methods to improve the health of cattle in the tropics: immunisation and chemoprophylaxis against haemoparasitic infections.	a study of methods to improve the health of native cattle in tropical areas of colombia showed an advantage using immunisation techniques against haemoparasitic infections in comparison with other control methods. the control of anaplasmosis and babesiosis by immunisation of cattle with fully virulent anaplasma marginale, babesia argentina and b. bigemina is feasible in tropical cattle when the postimmunisation reaction is controlled by appropriate drug therapy. chemoprophylaxis was found less e ...	1978	664017
survey of the incidence of anaplasmosis among nigerian zebu trade cattle.	sera samples from 573 nigerian zebu trade cattle were evaluated by the rapid card agglutination test for antibodies to anaplasma marginale between may and july 1977. the results showed 34 per cent reactors as against 66 per cent negatives. the above results showed a significant level of exposure to the infection among the fulani cattle managed under a husbandry system in which there is no provision for tick control. the significance of this level of exposure to anaplasma marginale in relation to ...	1978	664019
bovine abortion associated with anaplasma marginale.	during the period from june 1974 to june 1975, five bovine fetuses between seven to nine months old were received for necropsy from four different counties of the s. paulo state, brazil. all of them were from brucellosis-free herds. necropsy revealed slight liver enlargement generally accompanied by capsular petechial hemorrhages. enlargement and congestion of the spleen, epicardial and endocardial petechiae were present in three fetuses and one of them had lungs with some hemorrhagic lobules. c ...	1978	667708
immunization against anaplasmosis and babesiosis. part ii. evaluation of cryo-preserved vaccines using different doses and routes of inoculation.	anaplasma marginale, babesia argentina and babesia bigemina infected blood used as vaccines for immunization trials in valle del cauca, were preserved with 4 molar dimethyl-sulfoxide (4m dmso) and stored in liquid nitrogen (-196 degrees c). the effectivity of the vaccines was determined in 87 healthy calves utilizing serial 10-fold dilutions. the effects of dose, inoculation routes, time and temperature were determined. the minimum infective dose for a. marginale was 10(-3) (2 x 10(6)) when 2 ml ...	1978	675843
elimination of the carrier state of bovine anaplasmosis with a long-acting oxytetracycline.	a long-acting formulation of oxytetracycline (l-200) was injected at a dose level of 20 mg/kg intramuscularly into 12 yearling cattle that had been experimentally inoculated with anaplasma marginale 64 days previously. four cattle were treated twice, 4 were treated 3 times, and 4 were treated 4 times. all injections were given once ever 7 days. previous work (unpublished) with this formulation has revealed prolonged blood values following a single injection. the blood (80 ml) of each calf was in ...	1978	677530
a study of the epidemiology of anaplasma marginale infections of cattle in southern queensland: clinical disease and the prevalence of complement fixing antibodies.		1978	687261
some effects of reduced energy intake on the development of anaplasmosis in bos indicus cross steers.	some effects of the plane of nutrition on the development of anaplasmosis in brahman cross steers were investigated. batches of 39 and 30 brahman cross steers, aged approximately 27 months were each divided by stratified randomisation into 4 groups of similar mean pcv and body weight. two similar experiments, designated a and b were conducted. groups 1 and 2 were fed a ration of lucerne chaff at the rate of 1 m cal me/80 kg live weight/day for 8 weeks aimed to reduce body weight by approximately ...	1978	687262
experimental bovine anaplasmosis: clinico-pathological and nutritional studies.	groups of brahman-cross steers maintained on two nutritional planes were infected intravenously with a stabilate of anaplasma marginale. in general, animals on the higher plane of nutrition were more severely affected. fever was the first clinical sign of anaplasmosis but, like anaemia, was absent in the mildest cases. when present anaemia appeared two to three weeks after infection. there was a corresponding increase in erythrocyte sedimentation rates when read after 24 h but not at 1 h. the ha ...	1978	705052
methods to improve the health of cattle in the tropics: conclusions and economic appraisal.	the results of the immune responses of immunised and chemoprophylactically treated calves to tick-borne (boophilus microplus) challenge indicate that the system of immunisation was effective in protecting cattle against anaplasma marginale, babesia argentina (bovis), and b. bigemina. however, chemoprophylaxis was effective only against babesia spp. but not against a. marginale. both methods showed a substantial advantage over no control system when using native cattle breeds in a zone endemic fo ...	1978	705891
epidemiology of cattle anaplasmosis in colombia: i. prevalence and distribution of agglutinating antibodies.	a survey to detect antibodies against anaplasma marginale, using the card test, was conducted on serum samples collected from cattle of cordoba department and north-eastern region of the department of antioquia in the republic of colombia. the samples were from native cattle of spanish descent (bos taurus), zebu (bos indicus) and various zebu-native crosses. the age range was between three and eight year olds. the results revealed that 90.3% of the samples were positive. all breeds showed a high ...	1978	705897
serum levels of conglutinin, complement, and immunoconglutinin in cattle infected with anaplasma marginale.	sera from 10 splenectomized yearling cattle given massive doses of anaplasma marginale (peracute cases) were assayed for conglutinin and complement. sera from 5 nonsplenectomized yearling cattle given smaller doses (acute cases) were assayed for conglutinin, immunoconglutinin, and complement. in the peracute cases, conglutinating activity disappeared within 6 days after the cattle inoculated. in the acute cases, conglutining began to disappear at 3 weeks after inoculation and had disappeared at ...	1978	727576
synthesis of dna and protein by anaplasma marginale in bovine erythrocytes during short-term culture.	bovine erythrocytes infected with anaplasma marginale were cultured for 1 to 5 days in a co2 incubation chamber, pulse-labeled with [3h]thymidine and [14c]methionine, lysed, and fractionated by differential centrifugation and continuous density gradient centrifugation in renografin. anaplasma and associated fragments of stroma formed two distinct bands in the dense region of the gradient. electron microscopic examination of pelleted material from the bands from cells cultured for 1 day revealed ...	1978	730374
liquid nitrogen storage of anaplasma marginale complement-fixation antigen by a multiple small aliquot technique.	anaplasma marginale complement fixation test antigens were frozen as drops in liquid nitrogen using an easily constructed apparatus. economy in the use of antigen was achieved as the frozen drops were retrieved from storage in any required volume. the potency of the antigen was unaffected by the freezing and thawing technique. the technique is useful for storing other biological reagents.	1978	749095
amicarbalide: a therapeutic agent for anaplasmosis.	when administered subcutaneously in 2 equal daily doses at a total dosage rate of 20 mg/kg, amicarbalide was found to be an effective agent for controlling acute infections of anaplasma marginale and a. centrale in intact and splenectomized cattle. attempts to sterilize patent and latent anaplasma infections, however, were unsuccessful. at total dosage rates of 40 mg/kg and higher, amicarbalide exhibited potent hepato- and nephrotoxic tendencies.	1978	750960
ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet.	the localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. after potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. the localization of calcium on the membrane of the surface conn ...	1978	96043
comparisons of the complement-fixation, indirect fluorescent antibody, and card agglutination tests for the diagnosis of bovine anaplasmosis.	results of complement-fixation (cf), indirect fluorescent antibody (ifa), and card agglutination (ct) tests were statistically compared, using 380 serum samples obtained from 140 cattle which were disease-free or naturally or experimentally infected with anaplasma marginale of colombian origin. the ifa test was significantly the most sensitive for detection of amimals infected with anaplasmosis (97%); the ct test and the cf test were less so (84% and 79%, respectively). however, the most efficie ...	1978	358874
a comparison of 4 serological tests in the detection of humoral antibodies to anaplasmosis in cattle.	a capillary agglutination (ca), a complement fixation (cf), a plate agglutination (pt) and an indirect fluorescent antibody (ifa) test to detect humoral antibodies to anaplasma marginale are described. serums from 3, 4 or 5 groups of cattle were used to examine the efficiency of the tests. agreement between all 4 tests was 86.6%. agreement between pairs of tests was greater. the cf test was the most sensitive while the pt test was the least sensitive. however the pt could be carried out very rap ...	1978	363118
bronchial spasmolytic action of terbutaline and fenoterol compared with that of ipratropium bromide in the anaesthetized dog.	continuous recordings of heart rate, respiration rate, minute volume and bronchial resistance were made over a period of altogether three hours in spontaneously breathing dogs anaesthetized with chloralose. at hourly intervals, before and 1 and 2 hr after the administration of bronchodilators, bronchial spasm was induced by inhalation of an acetylcholine solution. two beta2-stimulants, terbutaline and fenoterol, were administered by inhalation in doses of 0.5, 0.16 and 0.05 mg/kg and 0.1, 0.3 an ...	1978	152096
endemic pneumocystis carinii in a marmoset colony.		1978	96156
comparison of kinetic and end-point diffusion methods for quantitating human serum immunoglobulins.	proficiency testing results were used to compare the kinetic and end-point versions of the single radial immunodiffusion method for quantitating human serum immunoglobulins. statistical analysis of the results indicated that the results were not normally distributed but that the log normal distribution gives the best fit of any of the well-known frequency distributions. consequently, statistical analysis of immunoglobulin results must be log transformed before parametric statistical tests can be ...	1978	97309
purification of myelin carbonic anhydrase.		1978	97371
effects of pelleting and sterilization of diet on 2 strains of rabbit coccidia.	both autoclaving and irradiation at levels currently used for other laboratory animal diets appear to be satisfactory for the elimination of 2 of the most robust and pathogenic species of rabbit coccidia. pelleting by the usual methods, even at 68 degrees c, was found to be unsatisfactory in eliminating all oocysts from heavily infected diet.	1978	97471
comutagenicity, competitive enzyme substrates, and in vitro carcinogenicity assays.		1978	97523
[aspects of the biochemistry of the urine in a newborn infant with "typical" valinuria and the effect of ingestion of valine on amino acid transport at the level of the brain in animals].		1978	97690
induction of arylhydrocarbon hydroxylase and udp glucuronosyl transferase by pcb in hepatic cell cultures.	the purpose of the investigation was to derive a hepatic cell line to be used in induction studies of drug metabolizing enzymes. two pure cell lines were isolated from primary liver cell cultures, one with an epithelial-like appearance, the other with a fibroblast-like appearance. the specific activities of arylhydrocarbon hydroxylase (ahh) and udp glucuronosyl transferase (udpgt) were greater in hepatocyte cultures than in primary cultures or in fibroblast cultures. pcb enhanced the activity of ...	1978	98133
[investigations of the incidence of neisseria gonorrhoeae on the pharyngeal mucosa (author's transl)].		1978	98235
[immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria (author's transl)].	an immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. more than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. its sensitivity compared well to the ifa test, although the antibodies revealed are quite different. a constnat precipitin line appeared, against 56 degrees c labile antigens, in sera of subjects infected by p. falciparum, and up to 8 o ...	1978	98748
guinea pig immunoglobulin light chain isotypes. i. separation of kappa and lambda chains and the identification of three isotypes of the lambda chain constant homology region.	the separation of intact kappa chain and two fragments comprising lambda (lambda) chain from immunoglobulin light chain pools isolated from strain 13 guinea pigs is achieved by cyanogen bromide digestion and gel filtration before and after reductive clevage of disulfide bonds. the smaller lambda chain fragment derives from the original carboxyl-terminus of lambda chain. the partial sequence of component tryptic and thermolytic peptides of this thirty-nine residue fragment allowed its complete se ...	1978	103729
congenital defects due to hyperthermia.		1978	104560
ophthalmic features of chromosome deletion 4p- (wolf-hirschhorn syndrome).	by using the giemsa banding technique we identified three patients with chromosome deletion 4p-. all had anterior segment anomalies, exotropia, blepharoptosis, antimongoloid palpebral fissures, hypertelorism, and disk abnormalities. one patient (case 1) had rieger's anomaly. some clinical features in patients with 4p- are similar to those in patients with chromosome deletion 5p-, cri-du-chat syndrome, although 4p- individuals do not have the distinctive cry. the ocular features which distinguish ...	1978	104624
specific labelling of replicating spp1 dna: analysis of viral dna synthesis and identification of phage dna-genes.	specific labelling of replicating bacteriophage spp1 dna can be achieved by infection at nonpermissive temperature of a b. subtilis strain carrying the initation mutation dnab ts134. under these conditions host dna synthesis is reduced by 90 to 95%. this technique was used to identify cistrons of spp1 involved in phage dna synthesis and to define intermediates in spp1 replication.	1978	105242
vaginal discharge and sexually transmitted diseases.		1978	108216
lay consultation by women with a lump in the breast.		1978	1277622
electron microscopic study of bacillus subtilis protoplast fusion.	when protoplasts derived from sporulating cells of bacillus subtilis were fused by exposure to polyethylene glycol (peg) and fixed immediately thereafter, protoplasts with two enclosed prespores could be seen by electron microscope. the number of fusion events was greatly increased, and multiply fused protoplasts appeared, when the peg-treated suspension was diluted in hypertonic broth and reincubated before fixation. this post-peg incubation effect is taken to indicate a fusion mechanism of two ...	1979	108247
thyrotropin-releasing hormone is not required for thyrotropin secretion in the perinatal rat.	to determine the role of thyrotropin-releasing hormone (trh) in the regulation of thyroid-stimulating hormone (tsh) secretion in the perinatal period, a physiological approach of neutralizing circulating trh in the fetal and early neonatal rat was employed. trh-antiserum (trh-as) raised in rabbits and administered daily to low iodine-propylthiouracil (lid-ptu)-fed pregnant rats from days 12 to 19 of gestation markedly impaired the rise in serum tsh to lid-ptu when compared with normal rabbit ser ...	1979	108290
experimental nasopharyngitis and pneumonia caused by chlamydia trachomatis in infant baboons: histopathologic comparison with a case in a human infant.	three infant male baboons were inoculated with a strain of chlamydia trachomatis isolated from a human infant with pneumonitis. one baboon, inoculated by intratracheal, nasopharyngeal, and oropharyngeal seeding, had rales, radiographic evidence of pneumonia, persistent nasopharyngeal c. trachomatis infection, and a four-fold rise in titer of antibody. at sacrifice 24 days after inoculation, nasopharynx, trachea, airways, and lung yielded c. trachomatis, and epithelial inclusions were seen by lig ...	1979	108341
3'-terminal nucleotide sequence of alfalfa mosaic virus rna 4.	the sequence of the 3'-terminal 91 nucleotides of alfalfa mosaic virus rna 4, the messenger for the viral coat protein, has been elucidated. a fragment containing the 3' terminus of the rna was obtained by mild digestion with rnase t1. the primary structure of the fragment was deduced by labeling it in vitro at its 5' terminus and application of rna sequencing techniques. the sequence is completely extracistronic and is believed to contain the binding sites for the viral coat protein and replica ...	1979	108677
myeloma proteins from nzb and balb/c mice: structural and functional differences.	structural and functional analyses of myeloma immunoglobulins from inbred balb/c mice and humans have provided important insights into the structure of the antibody molecule and the expression and evolution of antibody genes. one important question concerning these analyses is whether the myeloma process selects, in a nonrandom manner, the lymphocytes to be transformed. the availability of myeloma tumors in a second inbred strain of mouse, nzb, permits us to approach this question. in this respe ...	1979	108679
an explosive outbreak of influenza caused by a/ussr/77-like virus on a united states naval ship.		1979	119926
streptomycin sensitivity of ribosomes isolated from a streptomycin-producing streptomyces griseus.	the streptomycin sensitivity of ribosomes derived from a streptomycin-producing streptomyces griseus was examined in a polyuridylic acid directed 14c-phenylalanine incorporating system. in order to get reproducible results it is essential to use cell-free extracts which do not inactivate streptomycin. this condition can be fulfilled by the combination of washed ribosomes of the streptomycin-producing strain and the 110 000 g supernatant of the streptomycin-nonproducing variant of s. griseus, bec ...	1979	120678
effect of quantity and quality of igg antibodies on blocking of allergenic histamine release in vitro.	in a study of the protective role of igg antibodies in allergen injection therapy, serum antibodies to timothy grass allergens were raised in rabbits and tested for inhibition of histamine release from basophils of grass pollen-sensitive human subjects. rabbit sera were not inhibitory until late in the immune response when large amounts of relatively low affinity igg antibodies were detected by primary binding. inhibition of histamine release by late sera required preincubation of allergen and a ...	1979	84798
[current status of chemotherapy in malignant head and neck neoplasms (proceedings)].		1979	84806
fatal adenovirus infection with misleading positive serology for infectious mononucleosis.	symptoms and serological tests in a 19-year-old man with pneumonia indicated that he had infectious mononucleosis. adenovirus type 7 was demonstrated in the lung by electron microscopy and was cultured from the liver, lung, lymph-nodes, and kidney.	1979	84950
the patterns and distribution of euchrysine-binding grains in nonstimulated human peripheral t and non-t lymphocytes.		1979	86386
binding studies with antibodies having phosphorylcholine specificity and fragments derived from their homologous streptococcus pneumoniae type 27 capsular polysaccharide.	the capsular polysaccharide from streptococcus pneumoniae type 27 (s-27) has been fragmented into oligosaccharides by controlled degradations. binding of these derived small ligands with heterogeneous rabbit anti-s-27 antibody of selected phosphorylcholine affinity indicates that a major immunodeterminant of s-27 is the phosphorylcholine moiety, but that these anti-pc. antibodies are also capable of binding to the polysaccharide backbone from which the p.c. has been removed.	1979	87460
guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations.		1979	88292
studies on mononuclear phagocyte progenitor cells: morphology of cells and colonies in liquid culture of mouse bone marrow.		1979	88518
use of antibodies for studying the sidedness of membrane components.		1979	88659
the influenza virus: antigenic composition and immune response.	the architecture and chemical composition of the influenza virus particle is described with particular reference to the protein constituents and their genetic control. the dominant role in infection of the surface proteins - haemagglutinins and neuraminidases - acting as antigens and undergoing variation in time known as antigenic drift and shift is explained. the immuno-diffusion technique has illuminated the interrelationships of the haemagglutinins of influenza a viruses recovered over long p ...	1979	88726
reverse transcriptase-associated rnase h. part iv. pyrophosphate does not inhibit rnase h activity of amv dna polymerase.		1979	93479
amylase levels in the tissues and body fluids of the domestic cat (felis catus).	1. the amylase activities of serum, liver, pancreas, submaxillary and parotid glands, saliva, duodenum, colon, lung, heart, spleen, kidney and skeletal muscle of the domestic cat, were determined. 2. as in most mammals, the highest amylase content was found in the pancreas. levels in saliva and salivary glands were very low. 3. amylase activities of the serum, liver and pancreas have an optimum ph 7.0-7.5. 4. amylases of serum, liver and pancreas were inactivated by removal of chloride and these ...	1979	95689
serial prolactin and thyrotropin responses to thyrotropin-releasing hormone throughout normal human pregnancy.		1979	107178
interference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin.	although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. we investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. carrier-free 125i-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free fractions were separated. we demonstrated that diiodotyrosyl analogs bind avidly to serum proteins. because ...	1979	108034
seasonal variation and the influence of body temperature on plasma concentrations and binding of thyroxine and triiodothyronine in the woodchuck.	woodchuck plasma was collected during four seasons of the year and assayed for total and dialyzable (free) t4 and t3 and for rt3. plasma concentrations of total and free t4 and t3 were higher in the spring (t4, 5.4 +/- 0.6 microgram/dl; free t4, 3.0 +/- 0.4 ng/dl; t3, 202 +/- 22 ng/dl; free t3, 0.51 +/- 0.04 ng/dl) and lower in the prehibernatory fattening period in summer (t4, 2.3 +/- 1.0 microgram/dl; free t4, 1.2 +/- 0.5 ng/dl; t3, 45 +/- 27 ng/dl; free t3, 0.16 +/- 0.10 ng/dl) and fall (t4, ...	1979	108089
immunobiologic factors in the pathogenesis of renal infection.		1979	161989
human very low density lipoproteins stimulate triglyceride synthesis in rat adipose cells.		1979	219401
transformation by reticuloendotheliosis virus: development of a focus assay and isolation of a nontransforming virus.		1979	219596
serum angiotensin-converting enzyme, transcobalamin and lysozyme in normal and lepromatous armadillos.		1979	229221
comparison of the peroxidatic activity of cytochrome p-450 with other hemoproteins and model compounds.	the h2o2 dependent catalysis of cytochrome p-450 was compared with the catalytic mechanism of horse radish peroxidase, methemoglobin and iron protoporphyrin complexes. a relatively stable intermediate being comparable to compound i of horse radish peroxidase is formed in the case of iron porphyrin complexes, methemoglobin and probably cytochrome p-450. in the case of peroxidase compound ii is the more stable intermediate. this could be the reason for the different catalytic properties of peroxid ...	1979	229683
in vitro cultivation of anaplasma marginale: growth pattern and morphologic appearance.	anaplasma marginale propagated in vitro showed an increasing rate of replication in a sequence of three experiments. the changes in the percentage of parasitized erythrocytes and identification of the organism in culture were monitored by the giemsa-staining and the direct fluorescent antibody techniques. the ultrastructure of the organism in culture also was determined. the percentage of parasitized erythrocytes increased more than three times in the first experiment during a period of 8 days, ...	1979	230766
quantitative estimation of leishmanial antibody titers by enzyme-linked immunosorbent assay.	the response to immunization against leishmania braziliensis in rabbits was followed by measurement of antibody by enzyme-linked immunosorbent assay (elisa), passive hemagglutination, complement fixation, and countercurrent immunoelectrophoresis. passive hemagglutination and complement-fixation titers were interpreted according to standard methods, whereas elisa exact titers were derived by the equation y = a-b log x, which describes the straight line that results when absorbances are plotted ag ...	1979	387886
epizootiology and control of anaplasmosis in south africa.	the history of bovine anaplasmosis, or tick-born gallsickness, since the discovery of anaplasma marginale by sir arnold theiler is briefly reviewed. the development of the anaplasma centrale vaccine by theiler, up to the composition of the present vaccine issued by onderstepoort in which the original isolate is still passaged, is discussed in detail. recent transmission studies at onderstepoort have shown that 5 tick species are capable of transmitting anaplasmosis intrastadially, and intrastadi ...	1979	399978
characterization of the inclusion limiting membrane of anaplasma marginale by immunoferritin labeling.	purified anti-erythrocytic membrane antibody (pama) was prepared from rabbit anti-bovine erythrocyte serum by an adsorption and elution technique, utilizing bovine erythrocytes. lysed and washed anaplasma-infected erythrocytes were incubated with pama or control reagents. specimens were then subjected to immunoferritin labeling with ferritin antiglobulin conjugate. upon examination by electron microscopy, specimens incubated with pama showed heavy ferritin labeling of erythrocytic membranes and ...	1979	475129
evaluation of a new long-acting oxytetracycline formulation against anaplasmosis in colombian cattle.	a long-acting injectable (terramycin/la) forulated to contain 200 mg/ml of oxytetracycline was tested and compared to a commercially available oxytetracycline injectable (emicina) containing 50 mg/ml in the treatments of experimentally induced anaplasma marginale infection in colombian cattle. group 1, consisting of 10 infected calves, served as non-treated controls. ten infected calves (group 2) were treated with two doses of emicina, each at 10 mg/kg, intramuscularly (im), and another ten infe ...	1979	483386
evaluation of the anaplasmosis rapid card agglutination test for detecting experimentally-infected elk.	anaplasma marginale was experimentally transmitted from cattle to elk to cattle. six intact adult elk (cervus canadensis canadensis) inoculated with freshly collected heparinized blood from cattle chronically infected with a. marginale became asymptomatic carriers. although the elk did not develop clinical or hematologic evidence of infection, they became seropositive by the serum(srca) and plasma rapid card agglutination (prca) tests. blood from the experimentally-infected elk produced disease ...	1979	501840
in vitro cultivation of anaplasma marginale: invasion of and development in noninfected erythrocytes.	ovine erythrocytes infected with attenuated anaplasma marginale organisms were cultured in a suspension of normal ovine erythrocytes and normal bovine erythrocytes for 42 days. in each system, the organism showed an initial period of rapid growth followed by a gradual decrease in the percentage of parasitized erythrocytes accompanied by cyclic peaks. the percentage of infection of ovine erythrocytes were not different when normal ovine or bovine erythrocytes were added to the cultures. in vitro ...	1979	525898
electron microscope studies of anaplasma marginale in an aedes albopictus culture system.	anaplasma marginale was seen by electron microscopy within the cytoplasm of aedes albopictus cells in culture. anaplasma marginale was seen in free bovine rbc as well as in rbc phagocytized by ae albopictus cells. some a marginale organisms, both intracellular and extracellular, remained intact for as long as 60 days in culture, and anaplasma inclusion appendages were seen in free-lysed rbc and in lysed rbc phagocytized by ae albopictus cells. although some a marginale organisms had deteriorated ...	1979	525907
evaluation of the anaplasmosis rapid card agglutination test for detecting experimentally infected elk.	anaplasmosis was experimentally transmitted from cattle to elk to cattle. six non-splenectomized adult elk (cervus canadensis canadensis) that were inoculated with freshly collected heparinized blood from cattle chronically infected with anaplasma marginale became asymptomatic carriers. although the exposed elk did not develop clinical or hematologic evidence of infection they become seropositive by the serum and plasma rapid card agglutination (rca) tests. blood from the experimentally infected ...	1979	527345
reticulo-rumen motility in the chronically vagotomized sheep [proceedings].		1979	529098
voltage-clamp studies on the canine purkinje fibre [proceedings].		1979	529143
some observations on the effects of magnesium on the isolated perfused rat mesentery [proceedings].		1979	529150
[the experimental transmission of anaplasma marginale by the brown dog tick rhipicephalus sanguineus in australia].		1979	539952
[preparation and comparative testing of antigens for the immunodiagnosis of anaplasmosis in cattle].	three antigen types were produced by ultra-sound treatment of the initial material (anaplasma marginale theiler infected blood) and its subsequent sedimentional centrifugation from 10 000 to 100 000 g. their specificity and susceptibility to the reaction complement binding (rcb) and to the capillary agglutination (ca) serological tests was comparatively checked up. antigens a and b were very active in rcb, while antigen b--in the ca reaction. by these qualities they meet the requirements and are ...	1979	545841
the use of a short and a long acting oxytetracycline for the treatment of anaplasma marginale in splenectomized calves.	twenty splenectomized afrikander/simmentaler cross animals were used. eight of these were naturally infected with anaplasma marginale while 12 were infected artificially. when the packed cell volume was reduced to between 24% and 20% during the post splenectomy relapses or primary infections, six animals were treated with a single injection of 20 mg/kg of long acting oxytetracycline (la) and seven animals were treated on two successive days with 10 mg/kg of short acting oxytetracycline (sa). the ...	1979	551196
comparative efficacy of drugs in bovine anaplasmosis.	calves experimentally infected with anaplasma marginale were subjected to chemotherapeutic treatment in groups, each group receiving one drug only. observations were made on clinical signs, haematological changes, parasitaemias and levels of antibodies as measured by the complement fixation test. the most effective treatment was with imidocarb; other drugs which were apparently effective were steclin, terramycin and aureomycin in that order, while chloroquin diphosphate and camoquin gave little ...	1979	552672
effect of combined administration of furosemide and aspirin on urinary urate excretion in man.	furosemide (20 mg) was administered intravenously to 7 healthy volunteers, before and after 4 days of oral administration of aspirin in uricosuric dosage (1 g daily). aspirin prevented the decrease in urinary urate excretion induced by furosemide, without interfering with its natriuretic action. this finding is suggested to be the result of the opposite and independent effects of the two drugs on tubular reabsorption of urate.	1979	547103